Rh5BG039800

(2R)-phospho-3-sulfolactate synthase (ComA)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0007662
Physical Location & Seq
Forward (+)
2882437 .. 2890203
7767 bp
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UTR
Exon/CDS
Intron
Rh5BG039800.1

Sequence Viewer

Length: 648 bp
ATGTCGGAGTACCGATGGAAGACGTTCCACGAGAACGAAGATATTTTCGAGTCGATGGGCAACATTGTGTATGGGCTGAAGTTTGCTGGAGGTTCGCATAGCTTGATGCCAAAGCCGTTTGTGAAAGAAGTGATTGAGGTGGTTCACCAACATGACGTGTATGTAAGCACTGATGCTTGGGCTAAGAATTTGCTTCGAAAAGGCCCTTCGGCTTTTAAGGAGTATGTGGAGGAGTGTAAGAGCTTGGGATTTGACACAATTGAGCTGAATGTGGGATCTCTCGGAATGCCTGAGGAAACGCTTCTGAGACTTGTGCGCTTGATTAAGAGCGGTGGCTTGAAAGTGAAGCCTCTGTTTGAAGTCAAGGTTAACGAGTCTGACATTCCTATTGGTGATAGAGCGTTTGGAGCCTATGTTCGTCCCGAGACCAAGATCATCTGGTATGCTATGCACTCTTTCCTGAAACAGTTTCGGAACTTTTTTGATAATTGTTTTGCTGATGAGCAACAGATACTGCAATGGCAGACGAAATCCGAACCCCCTTCTTCGGTACATGATCAAAGACGATCTGCCAATTACAAGCCGAATATTTGGAAGTATGATTTCCTTGAATCTCTTAACAGCAAATTTGATGTGAGTTCTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ComA

Protein Analysis

215

Amino Acids

24.91

Weight (kDa)

6.1

Isoelectric Point (pI)

54.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ComA PF02679 10 - 127 4e-30 (2R)-phospho-3-sulfolactate synthase (ComA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 330
AciI CCGC 1 cut(s) 330
AclWI GGATC 1 cut(s) 283
AcsI RAATTY 2 cut(s) 187, 626
AcuI CTGAAG 1 cut(s) 98
AfaI GTAC 2 cut(s) 11, 552
AfiI CCNNNNNNNGG 1 cut(s) 547
AflIII ACRYGT 1 cut(s) 156
AgsI TTSAA 3 cut(s) 340, 359, 611
AjiI CACGTC 1 cut(s) 157
AloI GAACNNNNNNTCC 2 cut(s) 399, 431
AluBI AGCT 3 cut(s) 102, 243, 265
AluI AGCT 3 cut(s) 102, 243, 265
Alw26I GTCTC 2 cut(s) 301, 419
AlwI GGATC 1 cut(s) 283
AlwNI CAGNNNCTG 1 cut(s) 514
Ama87I CYCGRG 1 cut(s) 422
AoxI GGCC 1 cut(s) 202
ApoI RAATTY 2 cut(s) 187, 626
Asp700I GAANNNNTTC 2 cut(s) 23, 300
AspLEI GCGC 1 cut(s) 318
AspS9I GGNCC 1 cut(s) 203
AsuHPI GGTGA 2 cut(s) 137, 404
AsuII TTCGAA 1 cut(s) 196
AvaI CYCGRG 1 cut(s) 422
AxyI CCTNAGG 1 cut(s) 291
BauI CACGAG 1 cut(s) 29
BbsI GAAGAC 1 cut(s) 26
BccI CCATC 2 cut(s) 9, 49
BceAI ACGGC 1 cut(s) 100
BclI TGATCA 1 cut(s) 556
BcoDI GTCTC 2 cut(s) 301, 419
BmeT110I CYCGRG 1 cut(s) 422
BmgBI CACGTC 1 cut(s) 157
BmgT120I GGNCC 1 cut(s) 203
BmiI GGNNCC 1 cut(s) 409
BmsI GCATC 2 cut(s) 96, 163
BpiI GAAGAC 1 cut(s) 26
BpmI CTGGAG 1 cut(s) 108
Bpu14I TTCGAA 1 cut(s) 196
BsaI GGTCTC 1 cut(s) 419
BsaXI ACNNNNNCTCC 2 cut(s) 399, 429
Bsc4I CCNNNNNNNGG 1 cut(s) 547
Bse21I CCTNAGG 1 cut(s) 291
Bse3DI GCAATG 1 cut(s) 524
BseLI CCNNNNNNNGG 1 cut(s) 547
BseMI GCAATG 1 cut(s) 524
BseMII CTCAG 2 cut(s) 282, 296
BseRI GAGGAG 1 cut(s) 245
BshFI GGCC 1 cut(s) 204
BsiHKCI CYCGRG 1 cut(s) 422
BslFI GGGAC 1 cut(s) 405
BslI CCNNNNNNNGG 1 cut(s) 547
BsmAI GTCTC 2 cut(s) 301, 419
BsmFI GGGAC 1 cut(s) 405
BsmI GAATGC 1 cut(s) 291
BsnI GGCC 1 cut(s) 204
Bso31I GGTCTC 1 cut(s) 419
BsoBI CYCGRG 1 cut(s) 422
Bsp119I TTCGAA 1 cut(s) 196
Bsp143I GATC 4 cut(s) 275, 432, 556, 566
BspACI CCGC 1 cut(s) 330
BspANI GGCC 1 cut(s) 204
BspCNI CTCAG 2 cut(s) 283, 297
BspLI GGNNCC 1 cut(s) 409
BspPI GGATC 1 cut(s) 283
BspT104I TTCGAA 1 cut(s) 196
BspTNI GGTCTC 1 cut(s) 419
BsrBI CCGCTC 1 cut(s) 330
BsrDI GCAATG 1 cut(s) 524
BssMI GATC 4 cut(s) 275, 432, 556, 566
BssSI CACGAG 1 cut(s) 29
Bst2BI CACGAG 1 cut(s) 29
Bst4CI ACNGT 1 cut(s) 468
BstBI TTCGAA 1 cut(s) 196
BstDEI CTNAG 3 cut(s) 183, 291, 305
BstHHI GCGC 1 cut(s) 318
BstKTI GATC 4 cut(s) 278, 435, 559, 569
BstMAI GTCTC 2 cut(s) 301, 419
BstMBI GATC 4 cut(s) 275, 432, 556, 566
BstMWI GCNNNNNNNGC 1 cut(s) 407
BstV2I GAAGAC 1 cut(s) 26
BstX2I RGATCY 1 cut(s) 275
BstYI RGATCY 1 cut(s) 275
Bsu36I CCTNAGG 1 cut(s) 291
BsuRI GGCC 1 cut(s) 204
BtrI CACGTC 1 cut(s) 157
BtsIMutI CAGTG 1 cut(s) 168
CaiI CAGNNNCTG 1 cut(s) 514
CfoI GCGC 1 cut(s) 318
Cfr13I GGNCC 1 cut(s) 203
Csp6I GTAC 2 cut(s) 10, 551
CviAII CATG 2 cut(s) 152, 554
CviQI GTAC 2 cut(s) 10, 551
DdeI CTNAG 3 cut(s) 183, 291, 305
DpnI GATC 4 cut(s) 277, 434, 558, 568
DpnII GATC 4 cut(s) 275, 432, 556, 566
Eco31I GGTCTC 1 cut(s) 419
Eco57I CTGAAG 1 cut(s) 98
Eco81I CCTNAGG 1 cut(s) 291
Eco88I CYCGRG 1 cut(s) 422
EcoO109I RGGNCCY 1 cut(s) 203
FaeI CATG 2 cut(s) 155, 557
FaqI GGGAC 1 cut(s) 405
FatI CATG 2 cut(s) 151, 553
FbaI TGATCA 1 cut(s) 556
GlaI GCGC 1 cut(s) 317
GsuI CTGGAG 1 cut(s) 108
HaeIII GGCC 1 cut(s) 204
HhaI GCGC 1 cut(s) 318
Hin1II CATG 2 cut(s) 155, 557
Hin6I GCGC 1 cut(s) 316
HinP1I GCGC 1 cut(s) 316
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HinfI GANTC 3 cut(s) 50, 374, 611
HpaI GTTAAC 1 cut(s) 370
HphI GGTGA 2 cut(s) 137, 404
Hpy166II GTNNAC 2 cut(s) 145, 370
Hpy188I TCNGA 7 cut(s) 7, 284, 306, 379, 474, 535, 643
Hpy188III TCNNGA 2 cut(s) 422, 460
Hpy8I GTNNAC 2 cut(s) 145, 370
HpyAV CCTTC 2 cut(s) 216, 552
HpyCH4III ACNGT 1 cut(s) 468
HpyCH4IV ACGT 2 cut(s) 23, 156
HpyCH4V TGCA 2 cut(s) 451, 517
HpyF10VI GCNNNNNNNGC 1 cut(s) 407
HpyF3I CTNAG 3 cut(s) 183, 291, 305
HpySE526I ACGT 2 cut(s) 23, 156
Hsp92II CATG 2 cut(s) 155, 557
HspAI GCGC 1 cut(s) 316
Ksp22I TGATCA 1 cut(s) 556
KspAI GTTAAC 1 cut(s) 370
Kzo9I GATC 4 cut(s) 275, 432, 556, 566
LmnI GCTCC 1 cut(s) 407
LpnPI CCDG 4 cut(s) 72, 303, 424, 473
LweI GCATC 2 cut(s) 96, 163
MaeII ACGT 2 cut(s) 23, 156
MalI GATC 4 cut(s) 277, 434, 558, 568
MbiI CCGCTC 1 cut(s) 330
MboI GATC 4 cut(s) 275, 432, 556, 566
MboII GAAGA 3 cut(s) 31, 50, 537
MfeI CAATTG 1 cut(s) 258
MflI RGATCY 1 cut(s) 275
MluCI AATT 5 cut(s) 187, 258, 487, 574, 626
MlyI GAGTC 2 cut(s) 59, 383
MnlI CCTC 5 cut(s) 83, 130, 223, 286, 360
MroXI GAANNNNTTC 2 cut(s) 23, 300
MseI TTAA 4 cut(s) 216, 324, 369, 618
MslI CAYNNNNRTG 1 cut(s) 150
MunI CAATTG 1 cut(s) 258
Mva1269I GAATGC 1 cut(s) 291
MwoI GCNNNNNNNGC 1 cut(s) 407
NdeII GATC 4 cut(s) 275, 432, 556, 566
NlaIII CATG 2 cut(s) 155, 557
NlaIV GGNNCC 1 cut(s) 409
NspV TTCGAA 1 cut(s) 196
PctI GAATGC 1 cut(s) 291
PdmI GAANNNNTTC 2 cut(s) 23, 300
PfeI GAWTC 1 cut(s) 611
PleI GAGTC 2 cut(s) 58, 382
PpsI GAGTC 2 cut(s) 58, 382
PspN4I GGNNCC 1 cut(s) 409
PspPI GGNCC 1 cut(s) 203
PstNI CAGNNNCTG 1 cut(s) 514
PsuI RGATCY 1 cut(s) 275
RsaI GTAC 2 cut(s) 11, 552
RsaNI GTAC 2 cut(s) 10, 551
RseI CAYNNNNRTG 1 cut(s) 150
SaqAI TTAA 4 cut(s) 216, 324, 369, 618
Sau3AI GATC 4 cut(s) 275, 432, 556, 566
Sau96I GGNCC 1 cut(s) 203
SchI GAGTC 2 cut(s) 59, 383
SetI ASST 8 cut(s) 26, 94, 104, 141, 159, 245, 267, 369
SfaNI GCATC 2 cut(s) 96, 163
SfuI TTCGAA 1 cut(s) 196
SmiMI CAYNNNNRTG 1 cut(s) 150
Sse9I AATT 5 cut(s) 187, 258, 487, 574, 626
SsiI CCGC 1 cut(s) 330
SspI AATATT 1 cut(s) 589
TaaI ACNGT 1 cut(s) 468
TaiI ACGT 2 cut(s) 26, 159
TaqI TCGA 3 cut(s) 48, 53, 196
TasI AATT 5 cut(s) 187, 258, 487, 574, 626
TfiI GAWTC 1 cut(s) 611
Tru1I TTAA 4 cut(s) 216, 324, 369, 618
Tru9I TTAA 4 cut(s) 216, 324, 369, 618
TscAI CASTG 1 cut(s) 175
TspRI CASTG 1 cut(s) 175
XapI RAATTY 2 cut(s) 187, 626
XmnI GAANNNNTTC 2 cut(s) 23, 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.