FvH4_3g02960

(2R)-phospho-3-sulfolactate synthase (ComA)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0007662
Physical Location & Seq
Forward (+)
1571146 .. 1573912
2767 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g02960.t1

Sequence Viewer

Length: 846 bp
ATGTCGGCGTACAGATGGAAGACTTTCCACGAGAACGAGGATCGGCCGGAGAAGCCTCGCCGCTACGGCGTCACAGAAATGAGAGGCCCCGATCACTCTCTCTTAAGCCAAAACTTCCTTCAGGATATTTTCGAGTCGATGGGGGACATTGTGGATGGGCTGAGATTTGCTGGAGGTTCACATAGCTTGATGCCGAAGTCGTTTGTGAAAGAAGTGATTGATGAGGCTCACAAGCATGACGTGTATGTAAGCACCGGTAATTGGGCTGAGAATTTGCTCCGAAAAGGCCCTTCGGCTTTCAAAGAGTATGTGGAGGAGTGTAAGAGCTTGGGATTTGACACAATCGAGCTGAATGTGGGGTCGCTGGGAGTGCCGGAGGAAACGCTGCTGAGACTTGTGAGGTTGATTAAGAGCGGCGGATTGAGAGTGAAGCCTTCGTTTGCGGTTAAGGTTAACAAGTCTGACATTCCTGTTGGTGATAGAGCATTTGGAGCTTATGTGGTGCCGAGACCAAGGTCGTCTGATGTTGATCTGTTGATTAGAAGGGCTGAGAGATGTTTAGAGGCTGGGGCTGACATGATAATGATTGAGGCTGATGATGTCTGTAAACAAGCTGATTCAATGCGGGCAGACATAATTGCAAAGGTCATTGGGCGCCTTGGTGTGGAGAAGACCATGTTTGAAGCATCGAATCCAAGAACCTCAGAGTGGTTCATCCAGCATTATGGTCCAAGGGTCAATCTATTTGTGGATCACTCTCATGTGATGGACCTGGAGTGCCTTCGGGGACGTAACTTAGGTAGAAACCATGCATCTGTCTTGGGCTCCTCATATTTTCTGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ComA

Protein Analysis

282

Amino Acids

31.66

Weight (kDa)

6.52

Isoelectric Point (pI)

38.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ComA PF02679 14 - 265 3.6e-73 (2R)-phospho-3-sulfolactate synthase (ComA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 844
AccB1I GGYRCC 2 cut(s) 502, 654
AccBSI CCGCTC 1 cut(s) 414
AciI CCGC 5 cut(s) 61, 414, 417, 443, 625
AclWI GGATC 2 cut(s) 48, 759
AcoI YGGCCR 1 cut(s) 44
AcsI RAATTY 1 cut(s) 271
AcuI CTGAAG 1 cut(s) 104
AcyI GRCGYC 2 cut(s) 69, 655
AfaI GTAC 1 cut(s) 11
AfiI CCNNNNNNNGG 3 cut(s) 261, 664, 708
AflII CTTAAG 1 cut(s) 103
AflIII ACRYGT 1 cut(s) 240
AgeI ACCGGT 1 cut(s) 254
AgsI TTSAA 3 cut(s) 301, 621, 683
AjiI CACGTC 1 cut(s) 241
AjnI CCWGG 1 cut(s) 771
AjuI GAANNNNNNNTTGG 2 cut(s) 102, 134
AluBI AGCT 5 cut(s) 186, 327, 349, 494, 614
AluI AGCT 5 cut(s) 186, 327, 349, 494, 614
Alw26I GTCTC 2 cut(s) 385, 502
AlwI GGATC 2 cut(s) 48, 759
AoxI GGCC 3 cut(s) 44, 85, 286
ApeKI GCWGC 1 cut(s) 385
ApoI RAATTY 1 cut(s) 271
AsiGI ACCGGT 1 cut(s) 254
Asp700I GAANNNNTTC 1 cut(s) 23
AspLEI GCGC 1 cut(s) 657
AspS9I GGNCC 4 cut(s) 86, 287, 728, 769
AsuHPI GGTGA 1 cut(s) 488
AvaII GGWCC 2 cut(s) 728, 769
BanI GGYRCC 2 cut(s) 502, 654
BanII GRGCYC 1 cut(s) 827
BauI CACGAG 1 cut(s) 29
BbsI GAAGAC 2 cut(s) 26, 677
BbvI GCAGC 1 cut(s) 372
BccI CCATC 4 cut(s) 9, 133, 149, 760
BceAI ACGGC 1 cut(s) 82
BciT130I CCWGG 1 cut(s) 773
BcoDI GTCTC 2 cut(s) 385, 502
BfoI RGCGCY 1 cut(s) 658
BfrI CTTAAG 1 cut(s) 103
BglI GCCNNNNNGGC 1 cut(s) 66
BisI GCNGC 3 cut(s) 61, 386, 415
BlsI GCNGC 3 cut(s) 62, 387, 416
Bme1390I CCNGG 1 cut(s) 773
Bme18I GGWCC 2 cut(s) 728, 769
BmgBI CACGTC 1 cut(s) 241
BmgT120I GGNCC 4 cut(s) 86, 287, 728, 769
BmiI GGNNCC 4 cut(s) 88, 504, 656, 826
BmrFI CCNGG 1 cut(s) 773
BmsI GCATC 3 cut(s) 180, 695, 821
BoxI GACNNNNGTC 1 cut(s) 514
BpiI GAAGAC 2 cut(s) 26, 677
BpmI CTGGAG 2 cut(s) 192, 794
BsaBI GATNNNNATC 1 cut(s) 528
BsaHI GRCGYC 2 cut(s) 69, 655
BsaI GGTCTC 1 cut(s) 502
BsaJI CCNNGG 3 cut(s) 512, 658, 731
BsaWI WCCGGW 1 cut(s) 254
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bsc4I CCNNNNNNNGG 3 cut(s) 261, 664, 708
Bse118I RCCGGY 1 cut(s) 254
Bse8I GATNNNNATC 1 cut(s) 528
BseBI CCWGG 1 cut(s) 773
BseDI CCNNGG 3 cut(s) 512, 658, 731
BseGI GGATG 2 cut(s) 160, 714
BseJI GATNNNNATC 1 cut(s) 528
BseLI CCNNNNNNNGG 3 cut(s) 261, 664, 708
BseMII CTCAG 5 cut(s) 152, 258, 380, 540, 717
BseRI GAGGAG 2 cut(s) 329, 817
BseX3I CGGCCG 1 cut(s) 44
BseXI GCAGC 1 cut(s) 372
BseYI CCCAGC 2 cut(s) 364, 566
Bsh1285I CGRYCG 1 cut(s) 47
BshFI GGCC 3 cut(s) 46, 87, 288
BshNI GGYRCC 2 cut(s) 502, 654
BshTI ACCGGT 1 cut(s) 254
BsiEI CGRYCG 1 cut(s) 47
BsiSI CCGG 3 cut(s) 47, 255, 374
BslFI GGGAC 2 cut(s) 158, 801
BslI CCNNNNNNNGG 3 cut(s) 261, 664, 708
BsmAI GTCTC 2 cut(s) 385, 502
BsmFI GGGAC 2 cut(s) 158, 801
BsnI GGCC 3 cut(s) 46, 87, 288
Bso31I GGTCTC 1 cut(s) 502
Bsp1286I GDGCHC 1 cut(s) 827
Bsp143I GATC 4 cut(s) 40, 91, 529, 751
BspACI CCGC 5 cut(s) 61, 414, 417, 443, 625
BspANI GGCC 3 cut(s) 46, 87, 288
BspCNI CTCAG 5 cut(s) 153, 259, 381, 541, 716
BspLI GGNNCC 4 cut(s) 88, 504, 656, 826
BspPI GGATC 2 cut(s) 48, 759
BspT107I GGYRCC 2 cut(s) 502, 654
BspTI CTTAAG 1 cut(s) 103
BspTNI GGTCTC 1 cut(s) 502
BsrBI CCGCTC 1 cut(s) 414
BsrFI RCCGGY 1 cut(s) 254
BssAI RCCGGY 1 cut(s) 254
BssECI CCNNGG 3 cut(s) 512, 658, 731
BssMI GATC 4 cut(s) 40, 91, 529, 751
BssNI GRCGYC 2 cut(s) 69, 655
BssSI CACGAG 1 cut(s) 29
BssT1I CCWWGG 3 cut(s) 512, 658, 731
Bst2BI CACGAG 1 cut(s) 29
Bst2UI CCWGG 1 cut(s) 773
BstACI GRCGYC 2 cut(s) 69, 655
BstAFI CTTAAG 1 cut(s) 103
BstC8I GCNNGC 1 cut(s) 627
BstDEI CTNAG 6 cut(s) 161, 267, 389, 549, 703, 796
BstF5I GGATG 2 cut(s) 160, 714
BstH2I RGCGCY 1 cut(s) 658
BstHHI GCGC 1 cut(s) 657
BstKTI GATC 4 cut(s) 43, 94, 532, 754
BstMAI GTCTC 2 cut(s) 385, 502
BstMBI GATC 4 cut(s) 40, 91, 529, 751
BstMCI CGRYCG 1 cut(s) 47
BstMWI GCNNNNNNNGC 4 cut(s) 52, 66, 370, 491
BstNI CCWGG 1 cut(s) 773
BstPAI GACNNNNGTC 1 cut(s) 514
BstSCI CCNGG 1 cut(s) 771
BstV1I GCAGC 1 cut(s) 372
BstV2I GAAGAC 2 cut(s) 26, 677
BstXI CCANNNNNNTGG 1 cut(s) 725
BstZI CGGCCG 1 cut(s) 44
BsuRI GGCC 3 cut(s) 46, 87, 288
BtrI CACGTC 1 cut(s) 241
BtsCI GGATG 2 cut(s) 160, 714
Cac8I GCNNGC 1 cut(s) 627
CfoI GCGC 1 cut(s) 657
Cfr10I RCCGGY 1 cut(s) 254
Cfr13I GGNCC 4 cut(s) 86, 287, 728, 769
CseI GACGC 1 cut(s) 58
Csp6I GTAC 1 cut(s) 10
CspAI ACCGGT 1 cut(s) 254
CviAII CATG 5 cut(s) 236, 577, 676, 761, 809
CviQI GTAC 1 cut(s) 10
DdeI CTNAG 6 cut(s) 161, 267, 389, 549, 703, 796
DinI GGCGCC 1 cut(s) 656
DpnI GATC 4 cut(s) 42, 93, 531, 753
DpnII GATC 4 cut(s) 40, 91, 529, 751
EaeI YGGCCR 1 cut(s) 44
EagI CGGCCG 1 cut(s) 44
EciI GGCGGA 1 cut(s) 432
EclXI CGGCCG 1 cut(s) 44
Eco130I CCWWGG 3 cut(s) 512, 658, 731
Eco24I GRGCYC 1 cut(s) 827
Eco31I GGTCTC 1 cut(s) 502
Eco47I GGWCC 2 cut(s) 728, 769
Eco52I CGGCCG 1 cut(s) 44
Eco57I CTGAAG 1 cut(s) 104
EcoO109I RGGNCCY 2 cut(s) 86, 287
EcoRII CCWGG 1 cut(s) 771
EcoT14I CCWWGG 3 cut(s) 512, 658, 731
EcoT22I ATGCAT 1 cut(s) 814
EcoT38I GRGCYC 1 cut(s) 827
EgeI GGCGCC 1 cut(s) 656
EheI GGCGCC 1 cut(s) 656
ErhI CCWWGG 3 cut(s) 512, 658, 731
FaeI CATG 5 cut(s) 239, 580, 679, 764, 812
FaqI GGGAC 2 cut(s) 158, 801
FatI CATG 5 cut(s) 235, 576, 675, 760, 808
FauI CCCGC 1 cut(s) 618
Fnu4HI GCNGC 3 cut(s) 61, 386, 415
FokI GGATG 2 cut(s) 167, 701
FriOI GRGCYC 1 cut(s) 827
Fsp4HI GCNGC 3 cut(s) 61, 386, 415
GlaI GCGC 1 cut(s) 656
GluI GCNGC 3 cut(s) 61, 386, 415
GsaI CCCAGC 2 cut(s) 368, 570
GsuI CTGGAG 2 cut(s) 192, 794
HaeII RGCGCY 1 cut(s) 658
HaeIII GGCC 3 cut(s) 46, 87, 288
HapII CCGG 3 cut(s) 47, 255, 374
HgaI GACGC 1 cut(s) 58
HhaI GCGC 1 cut(s) 657
Hin1I GRCGYC 2 cut(s) 69, 655
Hin1II CATG 5 cut(s) 239, 580, 679, 764, 812
Hin6I GCGC 1 cut(s) 655
HinP1I GCGC 1 cut(s) 655
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HinfI GANTC 3 cut(s) 134, 617, 691
HpaI GTTAAC 1 cut(s) 454
HpaII CCGG 3 cut(s) 47, 255, 374
HphI GGTGA 1 cut(s) 488
Hpy166II GTNNAC 3 cut(s) 179, 454, 608
Hpy188I TCNGA 4 cut(s) 281, 463, 523, 706
Hpy188III TCNNGA 1 cut(s) 122
Hpy8I GTNNAC 3 cut(s) 179, 454, 608
HpyAV CCTTC 5 cut(s) 128, 300, 444, 537, 791
HpyCH4IV ACGT 2 cut(s) 240, 790
HpyCH4V TGCA 2 cut(s) 641, 812
HpyF10VI GCNNNNNNNGC 4 cut(s) 52, 66, 370, 491
HpyF3I CTNAG 6 cut(s) 161, 267, 389, 549, 703, 796
HpySE526I ACGT 2 cut(s) 240, 790
Hsp92I GRCGYC 2 cut(s) 69, 655
Hsp92II CATG 5 cut(s) 239, 580, 679, 764, 812
HspAI GCGC 1 cut(s) 655
KasI GGCGCC 1 cut(s) 654
KspAI GTTAAC 1 cut(s) 454
Kzo9I GATC 4 cut(s) 40, 91, 529, 751
LmnI GCTCC 3 cut(s) 282, 491, 830
Lsp1109I GCAGC 1 cut(s) 372
LweI GCATC 3 cut(s) 180, 695, 821
MaeII ACGT 2 cut(s) 240, 790
MaeIII GTNAC 2 cut(s) 70, 791
MalI GATC 4 cut(s) 42, 93, 531, 753
MbiI CCGCTC 1 cut(s) 414
MboI GATC 4 cut(s) 40, 91, 529, 751
MboII GAAGA 2 cut(s) 31, 682
MhlI GDGCHC 1 cut(s) 827
MluCI AATT 3 cut(s) 259, 271, 636
Mly113I GGCGCC 1 cut(s) 655
MlyI GAGTC 1 cut(s) 143
Mph1103I ATGCAT 1 cut(s) 814
MroXI GAANNNNTTC 1 cut(s) 23
MseI TTAA 4 cut(s) 104, 408, 447, 453
MslI CAYNNNNRTG 4 cut(s) 77, 234, 581, 759
MspCI CTTAAG 1 cut(s) 103
MspI CCGG 3 cut(s) 47, 255, 374
MspR9I CCNGG 1 cut(s) 773
MvaI CCWGG 1 cut(s) 773
MwoI GCNNNNNNNGC 4 cut(s) 52, 66, 370, 491
NarI GGCGCC 1 cut(s) 655
NdeII GATC 4 cut(s) 40, 91, 529, 751
NlaIII CATG 5 cut(s) 239, 580, 679, 764, 812
NlaIV GGNNCC 4 cut(s) 88, 504, 656, 826
NmeAIII GCCGAG 1 cut(s) 531
NmuCI GTSAC 1 cut(s) 70
NsiI ATGCAT 1 cut(s) 814
PdmI GAANNNNTTC 1 cut(s) 23
PfeI GAWTC 2 cut(s) 617, 691
PinAI ACCGGT 1 cut(s) 254
PkrI GCNGC 3 cut(s) 62, 387, 416
PleI GAGTC 1 cut(s) 142
PluTI GGCGCC 1 cut(s) 658
PpsI GAGTC 1 cut(s) 142
PshAI GACNNNNGTC 1 cut(s) 514
PsiI TTATAA 1 cut(s) 844
Psp6I CCWGG 1 cut(s) 771
PspFI CCCAGC 2 cut(s) 364, 566
PspGI CCWGG 1 cut(s) 771
PspN4I GGNNCC 4 cut(s) 88, 504, 656, 826
PspPI GGNCC 4 cut(s) 86, 287, 728, 769
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
RseI CAYNNNNRTG 4 cut(s) 77, 234, 581, 759
SaqAI TTAA 4 cut(s) 104, 408, 447, 453
SatI GCNGC 3 cut(s) 61, 386, 415
Sau3AI GATC 4 cut(s) 40, 91, 529, 751
Sau96I GGNCC 4 cut(s) 86, 287, 728, 769
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 1 cut(s) 773
SduI GDGCHC 1 cut(s) 827
SfaNI GCATC 3 cut(s) 180, 695, 821
SfoI GGCGCC 1 cut(s) 656
SinI GGWCC 2 cut(s) 728, 769
SmiMI CAYNNNNRTG 4 cut(s) 77, 234, 581, 759
SmlI CTYRAG 1 cut(s) 103
SmoI CTYRAG 1 cut(s) 103
Sse9I AATT 3 cut(s) 259, 271, 636
SsiI CCGC 5 cut(s) 61, 414, 417, 443, 625
SspDI GGCGCC 1 cut(s) 654
StyD4I CCNGG 1 cut(s) 771
StyI CCWWGG 3 cut(s) 512, 658, 731
TaiI ACGT 2 cut(s) 243, 793
TaqI TCGA 4 cut(s) 132, 137, 345, 689
TasI AATT 3 cut(s) 259, 271, 636
TauI GCSGC 2 cut(s) 63, 417
TfiI GAWTC 2 cut(s) 617, 691
Tru1I TTAA 4 cut(s) 104, 408, 447, 453
Tru9I TTAA 4 cut(s) 104, 408, 447, 453
TseFI GTSAC 1 cut(s) 70
TseI GCWGC 1 cut(s) 385
Tsp45I GTSAC 1 cut(s) 70
TspDTI ATGAA 1 cut(s) 703
Vha464I CTTAAG 1 cut(s) 103
VpaK11BI GGWCC 2 cut(s) 728, 769
XapI RAATTY 1 cut(s) 271
XmnI GAANNNNTTC 1 cut(s) 23
Zsp2I ATGCAT 1 cut(s) 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.