Rh5DG039400

(2R)-phospho-3-sulfolactate synthase (ComA)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0007662
Physical Location & Seq
Forward (+)
2875891 .. 2876735
845 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG039400.1

Sequence Viewer

Length: 456 bp
ATGTCGGAGTACCGATGGAAGACGTTCCACGAGAACGAAGATATTTTCGAGTCGATGGGCAACATTGTGTATGGGCTGAAGTTTGCTGGAGGTTCGCATAACTTGATGCCAAAGCCGTTTGTGAAAGAAGTGATTGATGTGGTTCACCAACATGACGTGTATGTAAGCACTGATGATTGGACTAAGAATTTGCTTCGAAAAGGCCCTTCGGCTTTTAAGGAGTATGTGGAGGAGTGTAAGAGCTTGGGATTTGACACAATTGAGCTGAATGTGGGATCTCTCGGAGTGCCTGAGGAAACGCTTCTGAGACTTGTGCGCTTGATTAAGAGCGGTGGCTTGAAAGTGAAGCCTCTGTTTGAAGTCAAGGTTAACGAGTCTGACATTCCTATTGGTGATAGAGCGTTTGGAGCCTATGTCGTCCCGAGACCAAGATCATCTGGACGTACGTTTGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ComA

Protein Analysis

151

Amino Acids

17.06

Weight (kDa)

6.43

Isoelectric Point (pI)

43.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ComA PF02679 10 - 127 3.1e-30 (2R)-phospho-3-sulfolactate synthase (ComA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 330
AciI CCGC 1 cut(s) 330
AclWI GGATC 1 cut(s) 283
AcsI RAATTY 1 cut(s) 187
AcuI CTGAAG 1 cut(s) 98
AfaI GTAC 2 cut(s) 11, 445
AflIII ACRYGT 1 cut(s) 156
AgsI TTSAA 2 cut(s) 340, 359
AjiI CACGTC 1 cut(s) 157
AluBI AGCT 2 cut(s) 243, 265
AluI AGCT 2 cut(s) 243, 265
Alw26I GTCTC 2 cut(s) 301, 418
AlwI GGATC 1 cut(s) 283
Ama87I CYCGRG 1 cut(s) 421
AoxI GGCC 1 cut(s) 202
ApoI RAATTY 1 cut(s) 187
ArsI GACNNNNNNTTYG 2 cut(s) 172, 204
Asp700I GAANNNNTTC 2 cut(s) 23, 300
AspLEI GCGC 1 cut(s) 318
AspS9I GGNCC 1 cut(s) 203
AsuHPI GGTGA 2 cut(s) 137, 404
AsuII TTCGAA 1 cut(s) 196
AvaI CYCGRG 1 cut(s) 421
AxyI CCTNAGG 1 cut(s) 291
BauI CACGAG 1 cut(s) 29
BbsI GAAGAC 1 cut(s) 26
BccI CCATC 2 cut(s) 9, 49
BceAI ACGGC 1 cut(s) 100
BcoDI GTCTC 2 cut(s) 301, 418
BmeT110I CYCGRG 1 cut(s) 421
BmgBI CACGTC 1 cut(s) 157
BmgT120I GGNCC 1 cut(s) 203
BmiI GGNNCC 1 cut(s) 409
BmsI GCATC 1 cut(s) 96
BpiI GAAGAC 1 cut(s) 26
BpmI CTGGAG 1 cut(s) 108
Bpu14I TTCGAA 1 cut(s) 196
BsaI GGTCTC 1 cut(s) 418
BsaXI ACNNNNNCTCC 2 cut(s) 399, 429
Bse21I CCTNAGG 1 cut(s) 291
BseMII CTCAG 2 cut(s) 282, 296
BseRI GAGGAG 1 cut(s) 245
BshFI GGCC 1 cut(s) 204
BsiHKCI CYCGRG 1 cut(s) 421
BsiWI CGTACG 1 cut(s) 443
BslFI GGGAC 1 cut(s) 404
BsmAI GTCTC 2 cut(s) 301, 418
BsmFI GGGAC 1 cut(s) 404
BsnI GGCC 1 cut(s) 204
Bso31I GGTCTC 1 cut(s) 418
BsoBI CYCGRG 1 cut(s) 421
Bsp119I TTCGAA 1 cut(s) 196
Bsp143I GATC 2 cut(s) 275, 431
BspACI CCGC 1 cut(s) 330
BspANI GGCC 1 cut(s) 204
BspCNI CTCAG 2 cut(s) 283, 297
BspLI GGNNCC 1 cut(s) 409
BspPI GGATC 1 cut(s) 283
BspT104I TTCGAA 1 cut(s) 196
BspTNI GGTCTC 1 cut(s) 418
BsrBI CCGCTC 1 cut(s) 330
BssMI GATC 2 cut(s) 275, 431
BssSI CACGAG 1 cut(s) 29
Bst2BI CACGAG 1 cut(s) 29
BstBI TTCGAA 1 cut(s) 196
BstDEI CTNAG 3 cut(s) 183, 291, 305
BstHHI GCGC 1 cut(s) 318
BstKTI GATC 2 cut(s) 278, 434
BstMAI GTCTC 2 cut(s) 301, 418
BstMBI GATC 2 cut(s) 275, 431
BstMWI GCNNNNNNNGC 1 cut(s) 407
BstV2I GAAGAC 1 cut(s) 26
BstX2I RGATCY 1 cut(s) 275
BstYI RGATCY 1 cut(s) 275
Bsu36I CCTNAGG 1 cut(s) 291
BsuRI GGCC 1 cut(s) 204
BtrI CACGTC 1 cut(s) 157
BtsIMutI CAGTG 1 cut(s) 168
CfoI GCGC 1 cut(s) 318
Cfr13I GGNCC 1 cut(s) 203
Csp6I GTAC 2 cut(s) 10, 444
CviAII CATG 1 cut(s) 152
CviJI RGCY 9 cut(s) 76, 115, 204, 212, 243, 265, 336, 349, 410
CviKI_1 RGCY 9 cut(s) 76, 115, 204, 212, 243, 265, 336, 349, 410
CviQI GTAC 2 cut(s) 10, 444
DdeI CTNAG 3 cut(s) 183, 291, 305
DpnI GATC 2 cut(s) 277, 433
DpnII GATC 2 cut(s) 275, 431
Eco31I GGTCTC 1 cut(s) 418
Eco57I CTGAAG 1 cut(s) 98
Eco81I CCTNAGG 1 cut(s) 291
Eco88I CYCGRG 1 cut(s) 421
EcoO109I RGGNCCY 1 cut(s) 203
FaeI CATG 1 cut(s) 155
FaiI YATR 6 cut(s) 72, 99, 153, 162, 225, 414
FaqI GGGAC 1 cut(s) 404
FatI CATG 1 cut(s) 151
GlaI GCGC 1 cut(s) 317
GsuI CTGGAG 1 cut(s) 108
HaeIII GGCC 1 cut(s) 204
HhaI GCGC 1 cut(s) 318
Hin1II CATG 1 cut(s) 155
Hin6I GCGC 1 cut(s) 316
HinP1I GCGC 1 cut(s) 316
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HinfI GANTC 2 cut(s) 50, 374
HpaI GTTAAC 1 cut(s) 370
HphI GGTGA 2 cut(s) 137, 404
Hpy166II GTNNAC 2 cut(s) 145, 370
Hpy188I TCNGA 4 cut(s) 7, 284, 306, 379
Hpy188III TCNNGA 2 cut(s) 421, 438
Hpy8I GTNNAC 2 cut(s) 145, 370
HpyAV CCTTC 1 cut(s) 216
HpyCH4IV ACGT 4 cut(s) 23, 156, 442, 446
HpyF10VI GCNNNNNNNGC 1 cut(s) 407
HpyF3I CTNAG 3 cut(s) 183, 291, 305
HpySE526I ACGT 4 cut(s) 23, 156, 442, 446
Hsp92II CATG 1 cut(s) 155
HspAI GCGC 1 cut(s) 316
KspAI GTTAAC 1 cut(s) 370
Kzo9I GATC 2 cut(s) 275, 431
LmnI GCTCC 1 cut(s) 407
LpnPI CCDG 3 cut(s) 72, 303, 423
LweI GCATC 1 cut(s) 96
MaeII ACGT 4 cut(s) 23, 156, 442, 446
MalI GATC 2 cut(s) 277, 433
MbiI CCGCTC 1 cut(s) 330
MboI GATC 2 cut(s) 275, 431
MboII GAAGA 2 cut(s) 31, 50
MfeI CAATTG 1 cut(s) 258
MflI RGATCY 1 cut(s) 275
MluCI AATT 2 cut(s) 187, 258
MlyI GAGTC 2 cut(s) 59, 383
MnlI CCTC 4 cut(s) 83, 223, 286, 360
MroXI GAANNNNTTC 2 cut(s) 23, 300
MseI TTAA 3 cut(s) 216, 324, 369
MslI CAYNNNNRTG 1 cut(s) 150
MunI CAATTG 1 cut(s) 258
MwoI GCNNNNNNNGC 1 cut(s) 407
NdeII GATC 2 cut(s) 275, 431
NlaIII CATG 1 cut(s) 155
NlaIV GGNNCC 1 cut(s) 409
NspV TTCGAA 1 cut(s) 196
PdmI GAANNNNTTC 2 cut(s) 23, 300
Pfl23II CGTACG 1 cut(s) 443
PleI GAGTC 2 cut(s) 58, 382
PpsI GAGTC 2 cut(s) 58, 382
PspLI CGTACG 1 cut(s) 443
PspN4I GGNNCC 1 cut(s) 409
PspPI GGNCC 1 cut(s) 203
PsuI RGATCY 1 cut(s) 275
RsaI GTAC 2 cut(s) 11, 445
RsaNI GTAC 2 cut(s) 10, 444
RseI CAYNNNNRTG 1 cut(s) 150
SaqAI TTAA 3 cut(s) 216, 324, 369
Sau3AI GATC 2 cut(s) 275, 431
Sau96I GGNCC 1 cut(s) 203
SchI GAGTC 2 cut(s) 59, 383
SetI ASST 8 cut(s) 26, 94, 159, 245, 267, 369, 445, 449
SfaNI GCATC 1 cut(s) 96
SfuI TTCGAA 1 cut(s) 196
SmiMI CAYNNNNRTG 1 cut(s) 150
Sse9I AATT 2 cut(s) 187, 258
SsiI CCGC 1 cut(s) 330
TaiI ACGT 4 cut(s) 26, 159, 445, 449
TaqI TCGA 3 cut(s) 48, 53, 196
TasI AATT 2 cut(s) 187, 258
Tru1I TTAA 3 cut(s) 216, 324, 369
Tru9I TTAA 3 cut(s) 216, 324, 369
TscAI CASTG 1 cut(s) 175
TspRI CASTG 1 cut(s) 175
XapI RAATTY 1 cut(s) 187
XmnI GAANNNNTTC 2 cut(s) 23, 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.