Rh5BG040000

(2R)-phospho-3-sulfolactate synthase (ComA)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0007662
Physical Location & Seq
Forward (+)
2894071 .. 2896301
2231 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG040000.1

Sequence Viewer

Length: 858 bp
ATGTCGGCGTACCGATGGAAGTCCTTCCACGAGAACGAAGACCGGCCGGAGAAGCCTCGCCGGTACGGCGTCACAGAAATGAGAGGCCCCAATCACACTCTCTTGACCCAAAACGTCCTTCAGGATATTTTCGAGTCGATGGGGGACATTGTGGATGGGCTGAAGTTTGCTGGAGGTTCGCATAGCTTGATGCCGAAGTCGTTTGTTAAAGAGGTGATTGATGTGGCTCACCAACATAACGTGTATGTAAGCACTGGTGATTGGGCTGAGAATTTACTTCGAAAAGGCCCTTCGGCTTTTAAAGAGTATGTGGAGGAGTGTAAGAGCTTGGGATTTGACACAATTGAGCTGAATGTGGGATCTCTCGGAATGCCTGAGGAAACGCTTCTGAGACTTGTGCGCTTGATTAAGAGCGGTGGCTTGAAGGTGAAGCCTCTGTTTGAAGTCAAGGTTAACGAGTCTGACATTCCTATTGGTGATAGAGCGTTCGGAGCCTATGTCGTCCCGAGACCAAGATCATCTGAATTTGTTGAAGATGTTGATCTGTTGATTAGAAGGGCTGAGAGATGCTTAGAAGCTGGGGCTGACATGATAATGATCGAGGCTGATGATGTCTGTAAACAGGCTGATTCAATGCGGCCAGACATAATTGCAAAGGTCATTGGGCGCCTTGGTGTCGAGAAGACCATGTTTGAAGCATCAAATCCAAGAACCTCGGAGTGGTTCATCCAGCATTATGGTCCAAGGGTCAATCTGTTTGTGGACCACTCTCATGTGATGGACCTGGAGTGCCTCCGGGGACGCAACTTAGGTAGAAACCATGCTTCTGTGTTGGGCTCCTCGTATTTTCTGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ComA

Protein Analysis

285

Amino Acids

32.2

Weight (kDa)

5.89

Isoelectric Point (pI)

37.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ComA PF02679 14 - 269 8.5e-75 (2R)-phospho-3-sulfolactate synthase (ComA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 856
AccB1I GGYRCC 1 cut(s) 666
AccBSI CCGCTC 1 cut(s) 414
AciI CCGC 2 cut(s) 414, 637
AclWI GGATC 1 cut(s) 367
AcoI YGGCCR 2 cut(s) 44, 638
AcsI RAATTY 2 cut(s) 271, 524
AcuI CTGAAG 2 cut(s) 104, 182
AcyI GRCGYC 2 cut(s) 69, 667
AfaI GTAC 2 cut(s) 11, 65
AfiI CCNNNNNNNGG 1 cut(s) 720
AflIII ACRYGT 1 cut(s) 240
AgsI TTSAA 5 cut(s) 424, 443, 533, 633, 695
AjnI CCWGG 1 cut(s) 783
AjuI GAANNNNNNNTTGG 2 cut(s) 102, 134
AluBI AGCT 4 cut(s) 186, 327, 349, 578
AluI AGCT 4 cut(s) 186, 327, 349, 578
Alw26I GTCTC 2 cut(s) 385, 502
AlwI GGATC 1 cut(s) 367
Ama87I CYCGRG 1 cut(s) 505
AoxI GGCC 4 cut(s) 44, 85, 286, 638
ApoI RAATTY 2 cut(s) 271, 524
Asp700I GAANNNNTTC 2 cut(s) 23, 384
AspLEI GCGC 2 cut(s) 402, 669
AspS9I GGNCC 5 cut(s) 86, 287, 740, 763, 781
AsuC2I CCSGG 1 cut(s) 797
AsuHPI GGTGA 5 cut(s) 221, 226, 269, 439, 488
AsuII TTCGAA 1 cut(s) 280
AvaI CYCGRG 1 cut(s) 505
AvaII GGWCC 3 cut(s) 740, 763, 781
AxyI CCTNAGG 1 cut(s) 375
BanI GGYRCC 1 cut(s) 666
BanII GRGCYC 1 cut(s) 839
BauI CACGAG 1 cut(s) 29
BbsI GAAGAC 2 cut(s) 45, 689
BccI CCATC 4 cut(s) 9, 133, 149, 772
BceAI ACGGC 1 cut(s) 82
BciT130I CCWGG 1 cut(s) 785
BcnI CCSGG 1 cut(s) 797
BcoDI GTCTC 2 cut(s) 385, 502
BfoI RGCGCY 1 cut(s) 670
BglI GCCNNNNNGGC 1 cut(s) 66
BisI GCNGC 1 cut(s) 638
BlsI GCNGC 1 cut(s) 639
Bme1390I CCNGG 2 cut(s) 785, 797
Bme18I GGWCC 3 cut(s) 740, 763, 781
BmeT110I CYCGRG 1 cut(s) 505
BmgT120I GGNCC 5 cut(s) 86, 287, 740, 763, 781
BmiI GGNNCC 4 cut(s) 88, 493, 668, 838
BmrFI CCNGG 2 cut(s) 785, 797
BmsI GCATC 3 cut(s) 180, 557, 707
BpiI GAAGAC 2 cut(s) 45, 689
BpmI CTGGAG 2 cut(s) 192, 806
Bpu14I TTCGAA 1 cut(s) 280
BpuMI CCSGG 1 cut(s) 797
BsaBI GATNNNNATC 2 cut(s) 540, 596
BsaHI GRCGYC 2 cut(s) 69, 667
BsaI GGTCTC 1 cut(s) 502
BsaJI CCNNGG 4 cut(s) 670, 714, 743, 796
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bsc4I CCNNNNNNNGG 1 cut(s) 720
Bse118I RCCGGY 2 cut(s) 42, 60
Bse1I ACTGG 1 cut(s) 259
Bse21I CCTNAGG 1 cut(s) 375
Bse8I GATNNNNATC 2 cut(s) 540, 596
BseBI CCWGG 1 cut(s) 785
BseDI CCNNGG 4 cut(s) 670, 714, 743, 796
BseGI GGATG 2 cut(s) 160, 726
BseJI GATNNNNATC 2 cut(s) 540, 596
BseLI CCNNNNNNNGG 1 cut(s) 720
BseMII CTCAG 4 cut(s) 258, 366, 380, 552
BseNI ACTGG 1 cut(s) 259
BseRI GAGGAG 2 cut(s) 329, 829
BseX3I CGGCCG 1 cut(s) 44
BseYI CCCAGC 1 cut(s) 578
Bsh1285I CGRYCG 1 cut(s) 47
BshFI GGCC 4 cut(s) 46, 87, 288, 640
BshNI GGYRCC 1 cut(s) 666
BsiEI CGRYCG 1 cut(s) 47
BsiHKCI CYCGRG 1 cut(s) 505
BsiSI CCGG 4 cut(s) 43, 47, 61, 796
BslFI GGGAC 3 cut(s) 158, 488, 813
BslI CCNNNNNNNGG 1 cut(s) 720
BsmAI GTCTC 2 cut(s) 385, 502
BsmFI GGGAC 3 cut(s) 158, 488, 813
BsmI GAATGC 1 cut(s) 375
BsnI GGCC 4 cut(s) 46, 87, 288, 640
Bso31I GGTCTC 1 cut(s) 502
BsoBI CYCGRG 1 cut(s) 505
Bsp119I TTCGAA 1 cut(s) 280
Bsp1286I GDGCHC 1 cut(s) 839
Bsp143I GATC 4 cut(s) 359, 515, 541, 597
BspACI CCGC 2 cut(s) 414, 637
BspANI GGCC 4 cut(s) 46, 87, 288, 640
BspCNI CTCAG 4 cut(s) 259, 367, 381, 553
BspLI GGNNCC 4 cut(s) 88, 493, 668, 838
BspPI GGATC 1 cut(s) 367
BspT104I TTCGAA 1 cut(s) 280
BspT107I GGYRCC 1 cut(s) 666
BspTNI GGTCTC 1 cut(s) 502
BsrBI CCGCTC 1 cut(s) 414
BsrFI RCCGGY 2 cut(s) 42, 60
BsrI ACTGG 1 cut(s) 259
BssAI RCCGGY 2 cut(s) 42, 60
BssECI CCNNGG 4 cut(s) 670, 714, 743, 796
BssMI GATC 4 cut(s) 359, 515, 541, 597
BssNI GRCGYC 2 cut(s) 69, 667
BssSI CACGAG 1 cut(s) 29
BssT1I CCWWGG 2 cut(s) 670, 743
Bst2BI CACGAG 1 cut(s) 29
Bst2UI CCWGG 1 cut(s) 785
BstACI GRCGYC 2 cut(s) 69, 667
BstBI TTCGAA 1 cut(s) 280
BstDEI CTNAG 6 cut(s) 267, 375, 389, 561, 571, 808
BstF5I GGATG 2 cut(s) 160, 726
BstH2I RGCGCY 1 cut(s) 670
BstHHI GCGC 2 cut(s) 402, 669
BstKTI GATC 4 cut(s) 362, 518, 544, 600
BstMAI GTCTC 2 cut(s) 385, 502
BstMBI GATC 4 cut(s) 359, 515, 541, 597
BstMCI CGRYCG 1 cut(s) 47
BstMWI GCNNNNNNNGC 3 cut(s) 52, 66, 491
BstNI CCWGG 1 cut(s) 785
BstSCI CCNGG 2 cut(s) 783, 795
BstV2I GAAGAC 2 cut(s) 45, 689
BstX2I RGATCY 1 cut(s) 359
BstXI CCANNNNNNTGG 1 cut(s) 737
BstYI RGATCY 1 cut(s) 359
BstZI CGGCCG 1 cut(s) 44
Bsu36I CCTNAGG 1 cut(s) 375
BsuRI GGCC 4 cut(s) 46, 87, 288, 640
BtsCI GGATG 2 cut(s) 160, 726
BtsIMutI CAGTG 1 cut(s) 252
CfoI GCGC 2 cut(s) 402, 669
Cfr10I RCCGGY 2 cut(s) 42, 60
Cfr13I GGNCC 5 cut(s) 86, 287, 740, 763, 781
CseI GACGC 2 cut(s) 58, 810
Csp6I GTAC 2 cut(s) 10, 64
CviAII CATG 4 cut(s) 589, 688, 773, 821
CviQI GTAC 2 cut(s) 10, 64
DdeI CTNAG 6 cut(s) 267, 375, 389, 561, 571, 808
DinI GGCGCC 1 cut(s) 668
DpnI GATC 4 cut(s) 361, 517, 543, 599
DpnII GATC 4 cut(s) 359, 515, 541, 597
DraI TTTAAA 1 cut(s) 301
EaeI YGGCCR 2 cut(s) 44, 638
EagI CGGCCG 1 cut(s) 44
EclXI CGGCCG 1 cut(s) 44
Eco130I CCWWGG 2 cut(s) 670, 743
Eco24I GRGCYC 1 cut(s) 839
Eco31I GGTCTC 1 cut(s) 502
Eco47I GGWCC 3 cut(s) 740, 763, 781
Eco52I CGGCCG 1 cut(s) 44
Eco57I CTGAAG 2 cut(s) 104, 182
Eco81I CCTNAGG 1 cut(s) 375
Eco88I CYCGRG 1 cut(s) 505
EcoO109I RGGNCCY 2 cut(s) 86, 287
EcoRII CCWGG 1 cut(s) 783
EcoT14I CCWWGG 2 cut(s) 670, 743
EcoT38I GRGCYC 1 cut(s) 839
EgeI GGCGCC 1 cut(s) 668
EheI GGCGCC 1 cut(s) 668
ErhI CCWWGG 2 cut(s) 670, 743
FaeI CATG 4 cut(s) 592, 691, 776, 824
FaqI GGGAC 3 cut(s) 158, 488, 813
FatI CATG 4 cut(s) 588, 687, 772, 820
Fnu4HI GCNGC 1 cut(s) 638
FokI GGATG 2 cut(s) 167, 713
FriOI GRGCYC 1 cut(s) 839
Fsp4HI GCNGC 1 cut(s) 638
GlaI GCGC 2 cut(s) 401, 668
GluI GCNGC 1 cut(s) 638
GsaI CCCAGC 1 cut(s) 582
GsuI CTGGAG 2 cut(s) 192, 806
HaeII RGCGCY 1 cut(s) 670
HaeIII GGCC 4 cut(s) 46, 87, 288, 640
HapII CCGG 4 cut(s) 43, 47, 61, 796
HgaI GACGC 2 cut(s) 58, 810
HhaI GCGC 2 cut(s) 402, 669
Hin1I GRCGYC 2 cut(s) 69, 667
Hin1II CATG 4 cut(s) 592, 691, 776, 824
Hin6I GCGC 2 cut(s) 400, 667
HinP1I GCGC 2 cut(s) 400, 667
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HinfI GANTC 3 cut(s) 134, 458, 629
HpaI GTTAAC 1 cut(s) 454
HpaII CCGG 4 cut(s) 43, 47, 61, 796
HphI GGTGA 5 cut(s) 221, 226, 269, 439, 488
Hpy166II GTNNAC 3 cut(s) 454, 620, 763
Hpy188I TCNGA 6 cut(s) 368, 390, 463, 491, 523, 718
Hpy188III TCNNGA 4 cut(s) 103, 122, 505, 679
Hpy8I GTNNAC 3 cut(s) 454, 620, 763
HpyAV CCTTC 5 cut(s) 34, 128, 300, 418, 549
HpyCH4IV ACGT 2 cut(s) 114, 240
HpyCH4V TGCA 1 cut(s) 653
HpyF10VI GCNNNNNNNGC 3 cut(s) 52, 66, 491
HpyF3I CTNAG 6 cut(s) 267, 375, 389, 561, 571, 808
HpySE526I ACGT 2 cut(s) 114, 240
Hsp92I GRCGYC 2 cut(s) 69, 667
Hsp92II CATG 4 cut(s) 592, 691, 776, 824
HspAI GCGC 2 cut(s) 400, 667
KasI GGCGCC 1 cut(s) 666
KspAI GTTAAC 1 cut(s) 454
Kzo9I GATC 4 cut(s) 359, 515, 541, 597
LmnI GCTCC 2 cut(s) 491, 842
LweI GCATC 3 cut(s) 180, 557, 707
MaeII ACGT 2 cut(s) 114, 240
MaeIII GTNAC 1 cut(s) 70
MalI GATC 4 cut(s) 361, 517, 543, 599
MbiI CCGCTC 1 cut(s) 414
MboI GATC 4 cut(s) 359, 515, 541, 597
MboII GAAGA 3 cut(s) 50, 545, 694
MfeI CAATTG 1 cut(s) 342
MflI RGATCY 1 cut(s) 359
MhlI GDGCHC 1 cut(s) 839
MluCI AATT 4 cut(s) 271, 342, 524, 648
Mly113I GGCGCC 1 cut(s) 667
MlyI GAGTC 2 cut(s) 143, 467
MroXI GAANNNNTTC 2 cut(s) 23, 384
MseI TTAA 4 cut(s) 207, 300, 408, 453
MslI CAYNNNNRTG 3 cut(s) 77, 593, 771
MspI CCGG 4 cut(s) 43, 47, 61, 796
MspR9I CCNGG 2 cut(s) 785, 797
MunI CAATTG 1 cut(s) 342
Mva1269I GAATGC 1 cut(s) 375
MvaI CCWGG 1 cut(s) 785
MwoI GCNNNNNNNGC 3 cut(s) 52, 66, 491
NarI GGCGCC 1 cut(s) 667
NciI CCSGG 1 cut(s) 797
NdeII GATC 4 cut(s) 359, 515, 541, 597
NlaIII CATG 4 cut(s) 592, 691, 776, 824
NlaIV GGNNCC 4 cut(s) 88, 493, 668, 838
NmuCI GTSAC 1 cut(s) 70
NspV TTCGAA 1 cut(s) 280
PctI GAATGC 1 cut(s) 375
PdmI GAANNNNTTC 2 cut(s) 23, 384
PfeI GAWTC 1 cut(s) 629
PkrI GCNGC 1 cut(s) 639
PleI GAGTC 2 cut(s) 142, 466
PluTI GGCGCC 1 cut(s) 670
PpsI GAGTC 2 cut(s) 142, 466
PsiI TTATAA 1 cut(s) 856
Psp6I CCWGG 1 cut(s) 783
PspFI CCCAGC 1 cut(s) 578
PspGI CCWGG 1 cut(s) 783
PspN4I GGNNCC 4 cut(s) 88, 493, 668, 838
PspPI GGNCC 5 cut(s) 86, 287, 740, 763, 781
PsuI RGATCY 1 cut(s) 359
RsaI GTAC 2 cut(s) 11, 65
RsaNI GTAC 2 cut(s) 10, 64
RseI CAYNNNNRTG 3 cut(s) 77, 593, 771
SaqAI TTAA 4 cut(s) 207, 300, 408, 453
SatI GCNGC 1 cut(s) 638
Sau3AI GATC 4 cut(s) 359, 515, 541, 597
Sau96I GGNCC 5 cut(s) 86, 287, 740, 763, 781
SchI GAGTC 2 cut(s) 143, 467
ScrFI CCNGG 2 cut(s) 785, 797
SduI GDGCHC 1 cut(s) 839
SfaNI GCATC 3 cut(s) 180, 557, 707
SfoI GGCGCC 1 cut(s) 668
SfuI TTCGAA 1 cut(s) 280
SinI GGWCC 3 cut(s) 740, 763, 781
SmiMI CAYNNNNRTG 3 cut(s) 77, 593, 771
Sse9I AATT 4 cut(s) 271, 342, 524, 648
SsiI CCGC 2 cut(s) 414, 637
SspDI GGCGCC 1 cut(s) 666
StyD4I CCNGG 2 cut(s) 783, 795
StyI CCWWGG 2 cut(s) 670, 743
TaiI ACGT 2 cut(s) 117, 243
TaqI TCGA 5 cut(s) 132, 137, 280, 600, 678
TasI AATT 4 cut(s) 271, 342, 524, 648
TauI GCSGC 1 cut(s) 640
TfiI GAWTC 1 cut(s) 629
Tru1I TTAA 4 cut(s) 207, 300, 408, 453
Tru9I TTAA 4 cut(s) 207, 300, 408, 453
TscAI CASTG 1 cut(s) 259
TseFI GTSAC 1 cut(s) 70
Tsp45I GTSAC 1 cut(s) 70
TspDTI ATGAA 1 cut(s) 715
TspRI CASTG 1 cut(s) 259
VpaK11BI GGWCC 3 cut(s) 740, 763, 781
XapI RAATTY 2 cut(s) 271, 524
XmnI GAANNNNTTC 2 cut(s) 23, 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.