MD05G1335600.v1.1

Anthocyanidin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010753
Physical Location & Seq
Reverse (-)
45822010 .. 45824874
2865 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1335600.v1.1.491

Sequence Viewer

Length: 1020 bp
ATGGCCACCCAACAACCCATCTCAAAGAAGACGGCTTGCGTCGTCGGCGGCACCGGGTTCGTGGCGTCTCTGCTGGTCAAGCTGCTGCTCCAGAAGGGCTACGCCGTCAGAACCACCGTCAGAGACCCAGACAATCACAAGAAGGTCTCCCACCTCACATCACTACAAGAGTTGGGTGAGCTAGAGATTTTAGCAGGAGATCTGACTGATGAAGGGAGCTTCGATGCTCCGATAGCAGGTTGTGATCTTGTTTTCCATGTTGCAACCCCTGTCAACTTTGCCTCAGAGGACCCGGAGAACGACATGATCAAACCGGCAATCCAAGGGGTACTAAACGTTCTGAAATCGTGTGTGAAGGCCAAAACAGTTAAGCGTGTTGTTTTGACATCATCAGCTGCTACAGTTTCGATCAATACACTTGAGGGAACAGGTTTGGTCATGGACGAAAAAGATTGGAGTGATTTGGAGTTCTTGACTACTGTAAAGCCACCCACTTGGGGGTACCCTGCCTCCAAGACACTAGCTGAGAAGACAGCTTGGAAATTCGCCGAAGAGAACAATATTGATCTCATCACTGTGATCCCTTCTCTTATGGCTGGTCCTTCTCTCACTCCAGACGTCCCCAGCAGTATCGGCCTTGCCATGTCTTTAATCACAGGAAATGATTTCCTCATAAATATGGCCTTGAAAGGTATGCAAATGCTATCAGGTTCGATATCCATTTCACATGTGGAGGATGTCTGCCGGGCGCATATATTTTTGGCTGAGAAAGAATCTGCTTCTGGTCGGTACATTTGCTGTGCTGCCAACACCAGCGTTCCTGAGCTTGCTCGGTTCCTCAACAAAAGATACCCCCAGTACAAAGTCCCCACTGAGTTTGGAGATTTTCCGTCCAAGGCCAAATTGATCATCTCTTCGGAGAAGCTTATCAAGGAGGGGTTCGATTTTAAGTATGGCATCGAAGAAATATATGACCAAACTGTGGAGTACTTCAAGGCCAAGGGGTTGCTGCAGAACTAG

Protein Analysis

340

Amino Acids

36.87

Weight (kDa)

5.52

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 9 - 130 4.3e-11 NmrA-like family
Epimerase PF01370 13 - 260 1.3e-26 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 14 - 182 6.6e-18 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 14 - 275 6.1e-13 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 15 - 231 6.2e-14 Male sterility protein
NAD_binding_10 PF13460 16 - 134 3.7e-12 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 621
Acc36I ACCTGC 1 cut(s) 227
Acc65I GGTACC 1 cut(s) 501
AccB1I GGYRCC 2 cut(s) 50, 501
AccB7I CCANNNNNTGG 1 cut(s) 982
AciI CCGC 1 cut(s) 48
AclI AACGTT 1 cut(s) 336
AclWI GGATC 1 cut(s) 574
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 542
AcyI GRCGYC 2 cut(s) 65, 618
AfaI GTAC 5 cut(s) 330, 503, 791, 860, 989
AfiI CCNNNNNNNGG 4 cut(s) 236, 497, 498, 982
AflIII ACRYGT 1 cut(s) 727
AgsI TTSAA 2 cut(s) 688, 994
AluBI AGCT 8 cut(s) 82, 181, 219, 395, 524, 536, 826, 925
AluI AGCT 8 cut(s) 82, 181, 219, 395, 524, 536, 826, 925
Alw26I GTCTC 3 cut(s) 72, 117, 151
AlwI GGATC 1 cut(s) 574
AoxI GGCC 6 cut(s) 3, 357, 634, 681, 897, 996
ApeKI GCWGC 5 cut(s) 82, 85, 395, 803, 1009
ApoI RAATTY 1 cut(s) 542
Asp718I GGTACC 1 cut(s) 501
AspLEI GCGC 1 cut(s) 751
AspS9I GGNCC 2 cut(s) 289, 599
AsuC2I CCSGG 3 cut(s) 55, 293, 746
AsuHPI GGTGA 1 cut(s) 188
AvaII GGWCC 2 cut(s) 289, 599
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 50, 501
BbsI GAAGAC 2 cut(s) 35, 536
BbvI GCAGC 5 cut(s) 69, 72, 382, 790, 996
BccI CCATC 1 cut(s) 26
BceAI ACGGC 2 cut(s) 48, 89
BcgI CGANNNNNNTGC 2 cut(s) 40, 74
BclI TGATCA 2 cut(s) 306, 906
BcnI CCSGG 3 cut(s) 55, 293, 746
BcoDI GTCTC 3 cut(s) 72, 117, 151
BfaI CTAG 3 cut(s) 182, 521, 1018
BfmI CTRYAG 2 cut(s) 399, 1010
BfuAI ACCTGC 1 cut(s) 227
BglII AGATCT 1 cut(s) 199
BisI GCNGC 6 cut(s) 49, 83, 86, 396, 804, 1010
BlsI GCNGC 6 cut(s) 50, 84, 87, 397, 805, 1011
BmcAI AGTACT 1 cut(s) 989
Bme1390I CCNGG 3 cut(s) 55, 293, 746
Bme18I GGWCC 2 cut(s) 289, 599
BmgT120I GGNCC 2 cut(s) 289, 599
BmiI GGNNCC 4 cut(s) 52, 291, 503, 836
BmrFI CCNGG 3 cut(s) 55, 293, 746
BmrI ACTGGG 1 cut(s) 850
BmsI GCATC 2 cut(s) 214, 966
BmuI ACTGGG 1 cut(s) 850
BpiI GAAGAC 2 cut(s) 35, 536
BpmI CTGGAG 2 cut(s) 74, 597
Bpu10I CCTNAGC 1 cut(s) 822
BpuEI CTTGAG 1 cut(s) 440
BpuMI CCSGG 3 cut(s) 55, 293, 746
BsaHI GRCGYC 2 cut(s) 65, 618
BsaI GGTCTC 2 cut(s) 117, 151
BsaJI CCNNGG 3 cut(s) 322, 894, 999
BsaXI ACNNNNNCTCC 2 cut(s) 494, 524
Bsc4I CCNNNNNNNGG 4 cut(s) 236, 497, 498, 982
Bse118I RCCGGY 1 cut(s) 313
Bse1I ACTGG 1 cut(s) 856
BseDI CCNNGG 3 cut(s) 322, 894, 999
BseGI GGATG 1 cut(s) 742
BseLI CCNNNNNNNGG 4 cut(s) 236, 497, 498, 982
BseMII CTCAG 5 cut(s) 297, 516, 756, 813, 864
BseNI ACTGG 1 cut(s) 856
BseXI GCAGC 5 cut(s) 69, 72, 382, 790, 996
BseYI CCCAGC 1 cut(s) 623
BshFI GGCC 6 cut(s) 5, 359, 636, 683, 899, 998
BshNI GGYRCC 2 cut(s) 50, 501
BsiSI CCGG 4 cut(s) 54, 293, 314, 745
BslFI GGGAC 2 cut(s) 605, 851
BslI CCNNNNNNNGG 4 cut(s) 236, 497, 498, 982
BsmAI GTCTC 3 cut(s) 72, 117, 151
BsmBI CGTCTC 1 cut(s) 72
BsmFI GGGAC 2 cut(s) 605, 851
BsnI GGCC 6 cut(s) 5, 359, 636, 683, 899, 998
Bso31I GGTCTC 2 cut(s) 117, 151
Bsp143I GATC 7 cut(s) 199, 244, 306, 408, 565, 579, 906
BspACI CCGC 1 cut(s) 48
BspANI GGCC 6 cut(s) 5, 359, 636, 683, 899, 998
BspCNI CTCAG 5 cut(s) 296, 517, 757, 814, 865
BspLI GGNNCC 4 cut(s) 52, 291, 503, 836
BspMAI CTGCAG 1 cut(s) 1014
BspMI ACCTGC 1 cut(s) 227
BspPI GGATC 1 cut(s) 574
BspT107I GGYRCC 2 cut(s) 50, 501
BspTNI GGTCTC 2 cut(s) 117, 151
BsrFI RCCGGY 1 cut(s) 313
BsrI ACTGG 1 cut(s) 856
BssAI RCCGGY 1 cut(s) 313
BssECI CCNNGG 3 cut(s) 322, 894, 999
BssMI GATC 7 cut(s) 199, 244, 306, 408, 565, 579, 906
BssNI GRCGYC 2 cut(s) 65, 618
BssT1I CCWWGG 3 cut(s) 322, 894, 999
Bst4CI ACNGT 6 cut(s) 118, 367, 403, 481, 577, 982
Bst6I CTCTTC 2 cut(s) 546, 919
BstACI GRCGYC 2 cut(s) 65, 618
BstC8I GCNNGC 2 cut(s) 37, 828
BstDEI CTNAG 5 cut(s) 283, 525, 765, 822, 873
BstF5I GGATG 1 cut(s) 742
BstHHI GCGC 1 cut(s) 751
BstKTI GATC 7 cut(s) 202, 247, 309, 411, 568, 582, 909
BstMAI GTCTC 3 cut(s) 72, 117, 151
BstMBI GATC 7 cut(s) 199, 244, 306, 408, 565, 579, 906
BstMWI GCNNNNNNNGC 4 cut(s) 45, 79, 233, 633
BstNSI RCATGY 1 cut(s) 731
BstSCI CCNGG 3 cut(s) 53, 291, 744
BstSFI CTRYAG 2 cut(s) 399, 1010
BstV1I GCAGC 5 cut(s) 69, 72, 382, 790, 996
BstV2I GAAGAC 2 cut(s) 35, 536
BstX2I RGATCY 1 cut(s) 199
BstXI CCANNNNNNTGG 1 cut(s) 495
BstYI RGATCY 1 cut(s) 199
BsuRI GGCC 6 cut(s) 5, 359, 636, 683, 899, 998
BtsCI GGATG 1 cut(s) 742
BtsIMutI CAGTG 2 cut(s) 573, 870
BveI ACCTGC 1 cut(s) 227
Cac8I GCNNGC 2 cut(s) 37, 828
CfoI GCGC 1 cut(s) 751
Cfr10I RCCGGY 1 cut(s) 313
Cfr13I GGNCC 2 cut(s) 289, 599
CseI GACGC 2 cut(s) 28, 54
Csp6I GTAC 5 cut(s) 329, 502, 790, 859, 988
CspCI CAANNNNNGTGG 2 cut(s) 859, 894
CviAII CATG 5 cut(s) 257, 304, 439, 643, 728
CviQI GTAC 5 cut(s) 329, 502, 790, 859, 988
DdeI CTNAG 5 cut(s) 283, 525, 765, 822, 873
DpnI GATC 7 cut(s) 201, 246, 308, 410, 567, 581, 908
DpnII GATC 7 cut(s) 199, 244, 306, 408, 565, 579, 906
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 546, 919
EarI CTCTTC 2 cut(s) 546, 919
Eco130I CCWWGG 3 cut(s) 322, 894, 999
Eco31I GGTCTC 2 cut(s) 117, 151
Eco32I GATATC 1 cut(s) 717
Eco47I GGWCC 2 cut(s) 289, 599
EcoO109I RGGNCCY 1 cut(s) 289
EcoRV GATATC 1 cut(s) 717
EcoT14I CCWWGG 3 cut(s) 322, 894, 999
ErhI CCWWGG 3 cut(s) 322, 894, 999
Esp3I CGTCTC 1 cut(s) 72
FaeI CATG 5 cut(s) 260, 307, 442, 646, 731
FaqI GGGAC 2 cut(s) 605, 851
FatI CATG 5 cut(s) 256, 303, 438, 642, 727
FbaI TGATCA 2 cut(s) 306, 906
Fnu4HI GCNGC 6 cut(s) 49, 83, 86, 396, 804, 1010
FokI GGATG 1 cut(s) 749
Fsp4HI GCNGC 6 cut(s) 49, 83, 86, 396, 804, 1010
FspBI CTAG 3 cut(s) 182, 521, 1018
GlaI GCGC 1 cut(s) 750
GluI GCNGC 6 cut(s) 49, 83, 86, 396, 804, 1010
GsaI CCCAGC 1 cut(s) 627
GsuI CTGGAG 2 cut(s) 74, 597
HaeIII GGCC 6 cut(s) 5, 359, 636, 683, 899, 998
HapII CCGG 4 cut(s) 54, 293, 314, 745
HgaI GACGC 2 cut(s) 28, 54
HhaI GCGC 1 cut(s) 751
Hin1I GRCGYC 2 cut(s) 65, 618
Hin1II CATG 5 cut(s) 260, 307, 442, 646, 731
Hin6I GCGC 1 cut(s) 749
HinP1I GCGC 1 cut(s) 749
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 923
HinfI GANTC 1 cut(s) 773
HpaII CCGG 4 cut(s) 54, 293, 314, 745
HphI GGTGA 1 cut(s) 188
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 7 cut(s) 110, 122, 204, 231, 286, 342, 919
Hpy188III TCNNGA 4 cut(s) 91, 472, 614, 821
Hpy8I GTNNAC 1 cut(s) 274
Hpy99I CGWCG 2 cut(s) 44, 47
HpyAV CCTTC 6 cut(s) 88, 136, 206, 349, 594, 612
HpyCH4III ACNGT 6 cut(s) 118, 367, 403, 481, 577, 982
HpyCH4IV ACGT 2 cut(s) 336, 618
HpyCH4V TGCA 3 cut(s) 263, 697, 1012
HpyF10VI GCNNNNNNNGC 4 cut(s) 45, 79, 233, 633
HpyF3I CTNAG 5 cut(s) 283, 525, 765, 822, 873
HpySE526I ACGT 2 cut(s) 336, 618
Hsp92I GRCGYC 2 cut(s) 65, 618
Hsp92II CATG 5 cut(s) 260, 307, 442, 646, 731
HspAI GCGC 1 cut(s) 749
KpnI GGTACC 1 cut(s) 505
Ksp22I TGATCA 2 cut(s) 306, 906
Kzo9I GATC 7 cut(s) 199, 244, 306, 408, 565, 579, 906
LmnI GCTCC 3 cut(s) 93, 216, 232
Lsp1109I GCAGC 5 cut(s) 69, 72, 382, 790, 996
LweI GCATC 2 cut(s) 214, 966
MaeI CTAG 3 cut(s) 182, 521, 1018
MaeII ACGT 2 cut(s) 336, 618
MalI GATC 7 cut(s) 201, 246, 308, 410, 567, 581, 908
MboI GATC 7 cut(s) 199, 244, 306, 408, 565, 579, 906
MboII GAAGA 5 cut(s) 40, 541, 563, 906, 974
MflI RGATCY 1 cut(s) 199
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 542, 902
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 9 cut(s) 164, 280, 292, 415, 520, 680, 727, 848, 928
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 369, 650, 948
MslI CAYNNNNRTG 2 cut(s) 575, 677
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 395
MspI CCGG 4 cut(s) 54, 293, 314, 745
MspR9I CCNGG 3 cut(s) 55, 293, 746
MwoI GCNNNNNNNGC 4 cut(s) 45, 79, 233, 633
NciI CCSGG 3 cut(s) 55, 293, 746
NdeII GATC 7 cut(s) 199, 244, 306, 408, 565, 579, 906
NlaIII CATG 5 cut(s) 260, 307, 442, 646, 731
NlaIV GGNNCC 4 cut(s) 52, 291, 503, 836
NspI RCATGY 1 cut(s) 731
PciI ACATGT 1 cut(s) 727
PfeI GAWTC 1 cut(s) 773
PflMI CCANNNNNTGG 1 cut(s) 982
PkrI GCNGC 6 cut(s) 50, 84, 87, 397, 805, 1011
PpuMI RGGWCCY 1 cut(s) 289
PscI ACATGT 1 cut(s) 727
Psp1406I AACGTT 1 cut(s) 336
Psp5II RGGWCCY 1 cut(s) 289
PspFI CCCAGC 1 cut(s) 623
PspN4I GGNNCC 4 cut(s) 52, 291, 503, 836
PspPI GGNCC 2 cut(s) 289, 599
PspPPI RGGWCCY 1 cut(s) 289
PsrI GAACNNNNNNTAC 2 cut(s) 321, 353
PstI CTGCAG 1 cut(s) 1014
PsuI RGATCY 1 cut(s) 199
PvuII CAGCTG 1 cut(s) 395
RsaI GTAC 5 cut(s) 330, 503, 791, 860, 989
RsaNI GTAC 5 cut(s) 329, 502, 790, 859, 988
RseI CAYNNNNRTG 2 cut(s) 575, 677
SaqAI TTAA 3 cut(s) 369, 650, 948
SatI GCNGC 6 cut(s) 49, 83, 86, 396, 804, 1010
Sau3AI GATC 7 cut(s) 199, 244, 306, 408, 565, 579, 906
Sau96I GGNCC 2 cut(s) 289, 599
ScaI AGTACT 1 cut(s) 989
ScrFI CCNGG 3 cut(s) 55, 293, 746
SfaNI GCATC 2 cut(s) 214, 966
SfcI CTRYAG 2 cut(s) 399, 1010
SinI GGWCC 2 cut(s) 289, 599
SmiMI CAYNNNNRTG 2 cut(s) 575, 677
SmlI CTYRAG 1 cut(s) 419
SmoI CTYRAG 1 cut(s) 419
Sse9I AATT 2 cut(s) 542, 902
SsiI CCGC 1 cut(s) 48
SspI AATATT 1 cut(s) 562
SspMI CTAG 3 cut(s) 182, 521, 1018
StyD4I CCNGG 3 cut(s) 53, 291, 744
StyI CCWWGG 3 cut(s) 322, 894, 999
TaaI ACNGT 6 cut(s) 118, 367, 403, 481, 577, 982
TaiI ACGT 2 cut(s) 339, 621
TaqI TCGA 5 cut(s) 222, 407, 713, 942, 960
TasI AATT 2 cut(s) 542, 902
TatI WGTACW 2 cut(s) 858, 987
TauI GCSGC 1 cut(s) 51
TfiI GAWTC 1 cut(s) 773
Tru1I TTAA 3 cut(s) 369, 650, 948
Tru9I TTAA 3 cut(s) 369, 650, 948
TscAI CASTG 2 cut(s) 580, 877
TseI GCWGC 5 cut(s) 82, 85, 395, 803, 1009
TspDTI ATGAA 1 cut(s) 225
TspGWI ACGGA 1 cut(s) 879
TspRI CASTG 2 cut(s) 580, 877
Van91I CCANNNNNTGG 1 cut(s) 982
VpaK11BI GGWCC 2 cut(s) 289, 599
XapI RAATTY 1 cut(s) 542
XceI RCATGY 1 cut(s) 731
XcmI CCANNNNNNNNNTGG 1 cut(s) 727
XspI CTAG 3 cut(s) 182, 521, 1018
ZraI GACGTC 1 cut(s) 619
ZrmI AGTACT 1 cut(s) 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.