MD10G1311100.v1.1

Anthocyanidin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010753
Physical Location & Seq
Reverse (-)
39618540 .. 39622618
4079 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1311100.v1.1.491

Sequence Viewer

Length: 1020 bp
ATGGCCACCCAACAACCCATCTCAAAGAAGACGGCTTGCGTCGTCGGCGGAACCGGGTTCATGGCGTCTCTGCTGGTCAAGCTACTGCTCCAGAAGGGCTACGCCGTCAGAACCACCGTCAGAGACCCTGACAACCACAAGAAGGTCTCCCACCTCACAGCACTACAAGAGTTGGGTGAGCTAGAGATTTTGGCAGGAGATCTAACTGATGAAGGGAGCTTCGATGCCCCAATAGCAGGCTGTGATCTTGTTTTCCATGTTGCAACCCCTGTCAACTTTGCCTCACAGGACCCGGAGAATGATATGATCAAACCAGCAATCCAAGGGGTACTAAATGTTCTGAAGTCGTGCGTGAAAGCCAAAACAGTTAAGCGCGTTGTTTTGACATCATCAGCTGCTACAGTTTCGATCAATACACTTGAAGGAACAGGTTTGGTCGTGGATGAAAAGGATTGGAGTGATTTGGAGTTCTTGACTAATGTAAAGCCACCCACTTGGGGGTACCCTGCCTCCAAGACACTAGCTGAGAAGACAGCTTGGAAATTTGCCGAAGAAAACAACATTGATCTAATCACTGTGATCCCTTCTCTTATGGCCGGTCCTTCTCTCACTCCAGACGTTCCCAGCAGTATCGGCCTTGCCATGGCTTTAATCACAGGAGATGATTTCCTCATAAATATGGCCCTGAAAGGTATGCAAATGTTATCAGGTTCGATATCCATTGCACATGTGGAGGACGTCTGCCGGGCTCATATATTTTTGGCTGAGAAAGAATCTGCATCTGGTCGGTACATTTGCTGTGCTGCCAACACCGGCGTTCCTGAGCTTGCTAAGTTCCTCAACAAAAGATACCCCCAGTACAAAGTCCCCACTGAGTTTGGAGATTTTCCGTCTGAGGCCAAACTGATCATCTCTTCAGAGAAGCTTATCAAGGAGGGGTTCGATTTTAAGTATGGCATCGAAGAAATATATGACCAAACTGTGGAGTACTTCAAGGCAAAGGGGCTGCTGCAGAACTAG

Protein Analysis

340

Amino Acids

36.78

Weight (kDa)

5.29

Isoelectric Point (pI)

34.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 9 - 130 5.2e-11 NmrA-like family
Epimerase PF01370 13 - 260 1.8e-26 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 14 - 182 2.1e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 14 - 219 9.2e-12 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 15 - 212 4.8e-14 Male sterility protein
NAD_binding_10 PF13460 16 - 134 1.3e-11 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 741
Acc65I GGTACC 1 cut(s) 501
AccB1I GGYRCC 1 cut(s) 501
AccB7I CCANNNNNTGG 1 cut(s) 982
AccII CGCG 1 cut(s) 375
AciI CCGC 1 cut(s) 48
AclWI GGATC 1 cut(s) 574
AcoI YGGCCR 2 cut(s) 3, 594
AcsI RAATTY 1 cut(s) 542
AcuI CTGAAG 2 cut(s) 362, 900
AcyI GRCGYC 2 cut(s) 65, 738
AfaI GTAC 5 cut(s) 330, 503, 791, 860, 989
AfiI CCNNNNNNNGG 6 cut(s) 142, 236, 497, 498, 643, 982
AflIII ACRYGT 1 cut(s) 727
AgsI TTSAA 2 cut(s) 422, 994
AluBI AGCT 8 cut(s) 82, 181, 219, 395, 524, 536, 826, 925
AluI AGCT 8 cut(s) 82, 181, 219, 395, 524, 536, 826, 925
Alw26I GTCTC 3 cut(s) 72, 117, 151
AlwI GGATC 1 cut(s) 574
AoxI GGCC 5 cut(s) 3, 594, 634, 681, 897
ApeKI GCWGC 4 cut(s) 395, 803, 1006, 1009
ApoI RAATTY 1 cut(s) 542
Asp718I GGTACC 1 cut(s) 501
AspLEI GCGC 1 cut(s) 375
AspS9I GGNCC 3 cut(s) 289, 599, 682
AsuC2I CCSGG 3 cut(s) 55, 293, 746
AsuHPI GGTGA 1 cut(s) 188
AvaII GGWCC 2 cut(s) 289, 599
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 501
BanII GRGCYC 1 cut(s) 751
BbsI GAAGAC 2 cut(s) 35, 536
BbvI GCAGC 4 cut(s) 382, 790, 993, 996
BccI CCATC 1 cut(s) 26
BceAI ACGGC 2 cut(s) 48, 89
BclI TGATCA 2 cut(s) 306, 906
BcnI CCSGG 3 cut(s) 55, 293, 746
BcoDI GTCTC 3 cut(s) 72, 117, 151
BfaI CTAG 3 cut(s) 182, 521, 1018
BfmI CTRYAG 2 cut(s) 399, 1010
BglII AGATCT 1 cut(s) 199
BisI GCNGC 4 cut(s) 396, 804, 1007, 1010
BlsI GCNGC 4 cut(s) 397, 805, 1008, 1011
BmcAI AGTACT 1 cut(s) 989
Bme1390I CCNGG 3 cut(s) 55, 293, 746
Bme18I GGWCC 2 cut(s) 289, 599
BmgT120I GGNCC 3 cut(s) 289, 599, 682
BmiI GGNNCC 3 cut(s) 52, 291, 503
BmrFI CCNGG 3 cut(s) 55, 293, 746
BmrI ACTGGG 1 cut(s) 850
BmsI GCATC 3 cut(s) 214, 788, 966
BmuI ACTGGG 1 cut(s) 850
BpiI GAAGAC 2 cut(s) 35, 536
BpmI CTGGAG 2 cut(s) 74, 597
Bpu10I CCTNAGC 1 cut(s) 822
BpuMI CCSGG 3 cut(s) 55, 293, 746
BsaHI GRCGYC 2 cut(s) 65, 738
BsaI GGTCTC 2 cut(s) 117, 151
BsaJI CCNNGG 2 cut(s) 322, 642
BsaXI ACNNNNNCTCC 2 cut(s) 494, 524
Bsc4I CCNNNNNNNGG 6 cut(s) 142, 236, 497, 498, 643, 982
Bse118I RCCGGY 2 cut(s) 596, 812
Bse1I ACTGG 1 cut(s) 856
Bse3DI GCAATG 1 cut(s) 720
BseDI CCNNGG 2 cut(s) 322, 642
BseGI GGATG 1 cut(s) 448
BseLI CCNNNNNNNGG 6 cut(s) 142, 236, 497, 498, 643, 982
BseMI GCAATG 1 cut(s) 720
BseMII CTCAG 5 cut(s) 516, 756, 813, 864, 885
BseNI ACTGG 1 cut(s) 856
BseXI GCAGC 4 cut(s) 382, 790, 993, 996
BseYI CCCAGC 1 cut(s) 623
Bsh1236I CGCG 1 cut(s) 375
BshFI GGCC 5 cut(s) 5, 596, 636, 683, 899
BshNI GGYRCC 1 cut(s) 501
BsiSI CCGG 5 cut(s) 54, 293, 597, 745, 813
BslFI GGGAC 1 cut(s) 851
BslI CCNNNNNNNGG 6 cut(s) 142, 236, 497, 498, 643, 982
BsmAI GTCTC 3 cut(s) 72, 117, 151
BsmBI CGTCTC 1 cut(s) 72
BsmFI GGGAC 1 cut(s) 851
BsnI GGCC 5 cut(s) 5, 596, 636, 683, 899
Bso31I GGTCTC 2 cut(s) 117, 151
Bsp1286I GDGCHC 1 cut(s) 751
Bsp143I GATC 7 cut(s) 199, 244, 306, 408, 565, 579, 906
Bsp19I CCATGG 1 cut(s) 642
BspACI CCGC 1 cut(s) 48
BspANI GGCC 5 cut(s) 5, 596, 636, 683, 899
BspCNI CTCAG 5 cut(s) 517, 757, 814, 865, 886
BspFNI CGCG 1 cut(s) 375
BspLI GGNNCC 3 cut(s) 52, 291, 503
BspMAI CTGCAG 1 cut(s) 1014
BspPI GGATC 1 cut(s) 574
BspT107I GGYRCC 1 cut(s) 501
BspTNI GGTCTC 2 cut(s) 117, 151
BsrDI GCAATG 1 cut(s) 720
BsrFI RCCGGY 2 cut(s) 596, 812
BsrI ACTGG 1 cut(s) 856
BssAI RCCGGY 2 cut(s) 596, 812
BssECI CCNNGG 2 cut(s) 322, 642
BssMI GATC 7 cut(s) 199, 244, 306, 408, 565, 579, 906
BssNI GRCGYC 2 cut(s) 65, 738
BssT1I CCWWGG 2 cut(s) 322, 642
Bst4CI ACNGT 5 cut(s) 118, 367, 403, 577, 982
Bst6I CTCTTC 1 cut(s) 919
BstACI GRCGYC 2 cut(s) 65, 738
BstC8I GCNNGC 3 cut(s) 37, 238, 828
BstDEI CTNAG 6 cut(s) 525, 765, 822, 831, 873, 894
BstDSI CCRYGG 1 cut(s) 642
BstF5I GGATG 1 cut(s) 448
BstFNI CGCG 1 cut(s) 375
BstHHI GCGC 1 cut(s) 375
BstKTI GATC 7 cut(s) 202, 247, 309, 411, 568, 582, 909
BstMAI GTCTC 3 cut(s) 72, 117, 151
BstMBI GATC 7 cut(s) 199, 244, 306, 408, 565, 579, 906
BstMWI GCNNNNNNNGC 4 cut(s) 45, 79, 233, 633
BstNSI RCATGY 1 cut(s) 731
BstSCI CCNGG 3 cut(s) 53, 291, 744
BstSFI CTRYAG 2 cut(s) 399, 1010
BstUI CGCG 1 cut(s) 375
BstV1I GCAGC 4 cut(s) 382, 790, 993, 996
BstV2I GAAGAC 2 cut(s) 35, 536
BstX2I RGATCY 1 cut(s) 199
BstXI CCANNNNNNTGG 1 cut(s) 495
BstYI RGATCY 1 cut(s) 199
BsuRI GGCC 5 cut(s) 5, 596, 636, 683, 899
BtgI CCRYGG 1 cut(s) 642
BtsCI GGATG 1 cut(s) 448
BtsIMutI CAGTG 2 cut(s) 573, 870
Cac8I GCNNGC 3 cut(s) 37, 238, 828
CfoI GCGC 1 cut(s) 375
Cfr10I RCCGGY 2 cut(s) 596, 812
Cfr13I GGNCC 3 cut(s) 289, 599, 682
CseI GACGC 2 cut(s) 28, 54
Csp6I GTAC 5 cut(s) 329, 502, 790, 859, 988
CspCI CAANNNNNGTGG 2 cut(s) 859, 894
CviAII CATG 4 cut(s) 61, 257, 643, 728
CviQI GTAC 5 cut(s) 329, 502, 790, 859, 988
DdeI CTNAG 6 cut(s) 525, 765, 822, 831, 873, 894
DpnI GATC 7 cut(s) 201, 246, 308, 410, 567, 581, 908
DpnII GATC 7 cut(s) 199, 244, 306, 408, 565, 579, 906
EaeI YGGCCR 2 cut(s) 3, 594
Eam1104I CTCTTC 1 cut(s) 919
EarI CTCTTC 1 cut(s) 919
EciI GGCGGA 1 cut(s) 63
Eco130I CCWWGG 2 cut(s) 322, 642
Eco24I GRGCYC 1 cut(s) 751
Eco31I GGTCTC 2 cut(s) 117, 151
Eco32I GATATC 1 cut(s) 717
Eco47I GGWCC 2 cut(s) 289, 599
Eco57I CTGAAG 2 cut(s) 362, 900
EcoO109I RGGNCCY 1 cut(s) 289
EcoRV GATATC 1 cut(s) 717
EcoT14I CCWWGG 2 cut(s) 322, 642
EcoT38I GRGCYC 1 cut(s) 751
ErhI CCWWGG 2 cut(s) 322, 642
Esp3I CGTCTC 1 cut(s) 72
FaeI CATG 4 cut(s) 64, 260, 646, 731
FaqI GGGAC 1 cut(s) 851
FatI CATG 4 cut(s) 60, 256, 642, 727
FbaI TGATCA 2 cut(s) 306, 906
Fnu4HI GCNGC 4 cut(s) 396, 804, 1007, 1010
FokI GGATG 1 cut(s) 455
FriOI GRGCYC 1 cut(s) 751
Fsp4HI GCNGC 4 cut(s) 396, 804, 1007, 1010
FspBI CTAG 3 cut(s) 182, 521, 1018
GlaI GCGC 1 cut(s) 374
GluI GCNGC 4 cut(s) 396, 804, 1007, 1010
GsaI CCCAGC 1 cut(s) 627
GsuI CTGGAG 2 cut(s) 74, 597
HaeIII GGCC 5 cut(s) 5, 596, 636, 683, 899
HapII CCGG 5 cut(s) 54, 293, 597, 745, 813
HgaI GACGC 2 cut(s) 28, 54
HhaI GCGC 1 cut(s) 375
Hin1I GRCGYC 2 cut(s) 65, 738
Hin1II CATG 4 cut(s) 64, 260, 646, 731
Hin6I GCGC 1 cut(s) 373
HinP1I GCGC 1 cut(s) 373
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 923
HinfI GANTC 1 cut(s) 773
HpaII CCGG 5 cut(s) 54, 293, 597, 745, 813
HphI GGTGA 1 cut(s) 188
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 5 cut(s) 110, 122, 342, 895, 919
Hpy188III TCNNGA 4 cut(s) 91, 472, 614, 821
Hpy8I GTNNAC 1 cut(s) 274
Hpy99I CGWCG 2 cut(s) 44, 47
HpyAV CCTTC 6 cut(s) 88, 136, 206, 416, 594, 612
HpyCH4III ACNGT 5 cut(s) 118, 367, 403, 577, 982
HpyCH4IV ACGT 2 cut(s) 618, 738
HpyCH4V TGCA 5 cut(s) 263, 697, 725, 779, 1012
HpyF10VI GCNNNNNNNGC 4 cut(s) 45, 79, 233, 633
HpyF3I CTNAG 6 cut(s) 525, 765, 822, 831, 873, 894
HpySE526I ACGT 2 cut(s) 618, 738
Hsp92I GRCGYC 2 cut(s) 65, 738
Hsp92II CATG 4 cut(s) 64, 260, 646, 731
HspAI GCGC 1 cut(s) 373
KpnI GGTACC 1 cut(s) 505
Ksp22I TGATCA 2 cut(s) 306, 906
Kzo9I GATC 7 cut(s) 199, 244, 306, 408, 565, 579, 906
LmnI GCTCC 2 cut(s) 93, 216
Lsp1109I GCAGC 4 cut(s) 382, 790, 993, 996
LweI GCATC 3 cut(s) 214, 788, 966
MaeI CTAG 3 cut(s) 182, 521, 1018
MaeII ACGT 2 cut(s) 618, 738
MalI GATC 7 cut(s) 201, 246, 308, 410, 567, 581, 908
MboI GATC 7 cut(s) 199, 244, 306, 408, 565, 579, 906
MboII GAAGA 5 cut(s) 40, 541, 563, 906, 974
MflI RGATCY 1 cut(s) 199
MhlI GDGCHC 1 cut(s) 751
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 542
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 8 cut(s) 164, 292, 520, 680, 727, 848, 889, 928
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 369, 650, 948
MslI CAYNNNNRTG 1 cut(s) 677
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 395
MspI CCGG 5 cut(s) 54, 293, 597, 745, 813
MspR9I CCNGG 3 cut(s) 55, 293, 746
MvnI CGCG 1 cut(s) 375
MwoI GCNNNNNNNGC 4 cut(s) 45, 79, 233, 633
NciI CCSGG 3 cut(s) 55, 293, 746
NcoI CCATGG 1 cut(s) 642
NdeII GATC 7 cut(s) 199, 244, 306, 408, 565, 579, 906
NlaIII CATG 4 cut(s) 64, 260, 646, 731
NlaIV GGNNCC 3 cut(s) 52, 291, 503
NspI RCATGY 1 cut(s) 731
PciI ACATGT 1 cut(s) 727
PfeI GAWTC 1 cut(s) 773
PflMI CCANNNNNTGG 1 cut(s) 982
PkrI GCNGC 4 cut(s) 397, 805, 1008, 1011
PpuMI RGGWCCY 1 cut(s) 289
PscI ACATGT 1 cut(s) 727
Psp5II RGGWCCY 1 cut(s) 289
PspFI CCCAGC 1 cut(s) 623
PspN4I GGNNCC 3 cut(s) 52, 291, 503
PspPI GGNCC 3 cut(s) 289, 599, 682
PspPPI RGGWCCY 1 cut(s) 289
PsrI GAACNNNNNNTAC 2 cut(s) 321, 353
PstI CTGCAG 1 cut(s) 1014
PsuI RGATCY 1 cut(s) 199
PvuII CAGCTG 1 cut(s) 395
RsaI GTAC 5 cut(s) 330, 503, 791, 860, 989
RsaNI GTAC 5 cut(s) 329, 502, 790, 859, 988
RseI CAYNNNNRTG 1 cut(s) 677
SaqAI TTAA 3 cut(s) 369, 650, 948
SatI GCNGC 4 cut(s) 396, 804, 1007, 1010
Sau3AI GATC 7 cut(s) 199, 244, 306, 408, 565, 579, 906
Sau96I GGNCC 3 cut(s) 289, 599, 682
ScaI AGTACT 1 cut(s) 989
ScrFI CCNGG 3 cut(s) 55, 293, 746
SduI GDGCHC 1 cut(s) 751
SfaNI GCATC 3 cut(s) 214, 788, 966
SfcI CTRYAG 2 cut(s) 399, 1010
SgrAI CRCCGGYG 1 cut(s) 812
SinI GGWCC 2 cut(s) 289, 599
SmiMI CAYNNNNRTG 1 cut(s) 677
Sse9I AATT 1 cut(s) 542
SsiI CCGC 1 cut(s) 48
SspMI CTAG 3 cut(s) 182, 521, 1018
StyD4I CCNGG 3 cut(s) 53, 291, 744
StyI CCWWGG 2 cut(s) 322, 642
TaaI ACNGT 5 cut(s) 118, 367, 403, 577, 982
TaiI ACGT 2 cut(s) 621, 741
TaqI TCGA 5 cut(s) 222, 407, 713, 942, 960
TasI AATT 1 cut(s) 542
TatI WGTACW 2 cut(s) 858, 987
TfiI GAWTC 1 cut(s) 773
Tru1I TTAA 3 cut(s) 369, 650, 948
Tru9I TTAA 3 cut(s) 369, 650, 948
TscAI CASTG 2 cut(s) 580, 877
TseI GCWGC 4 cut(s) 395, 803, 1006, 1009
TspDTI ATGAA 3 cut(s) 49, 225, 459
TspGWI ACGGA 1 cut(s) 879
TspRI CASTG 2 cut(s) 580, 877
Van91I CCANNNNNTGG 1 cut(s) 982
VpaK11BI GGWCC 2 cut(s) 289, 599
XapI RAATTY 1 cut(s) 542
XceI RCATGY 1 cut(s) 731
XcmI CCANNNNNNNNNTGG 1 cut(s) 727
XspI CTAG 3 cut(s) 182, 521, 1018
ZraI GACGTC 1 cut(s) 739
ZrmI AGTACT 1 cut(s) 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.