Rorug04G0412500

Anthocyanidin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0010753
Physical Location & Seq
Reverse (-)
57457908 .. 57472513
14606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0412500.1

Sequence Viewer

Length: 3678 bp
ATGGCACTGGCAGTTTCAGCTGGCATGTGTTTCGGTGGAAATGGATTGAGAAGTGCAATAGCTTTTGAAGCTGAGAAGGGATATCGGAATGCATTGGGTAGCAAAATGAGGTTCAGTACTTTTCTCCTTTCCAAAGTATTGAAATTATGTTCTCGATCAAAGCATAACTTTGCTAAGAGTATTTTGGAGGAGGCAGATAGTTATGGCATAGCAAGCATCTCAGATCGGTCAAAGTTGCTTAACAAGGTATCTGTTTTGATGGGTTATGATGGTCTTCATGATTTAATTGAGAATGAAAGAGCTGAAAAGCATTATGGTATGAATTTTAAAGATGCAATGGATGATTTTGATGCCTCCTTTGTCTGCAAGAGATTTCCATCAATCATTTTGGGTGATTCCCCTCCGGTGGAATTGTATGATGGGACTGCTAATTTCTTTGAAAAAATGACACTTTTAACAACTCAAAGTCCTGAAGGATTTCCTTCTGGCTCTACGGTTGAAGAGCAGGAGGGAGATTATCTCTATGAAACAGGGGATTCTCTGTATCCTGCGTTTCCAAGTGAAGAAACTTCCTTGTTAACAGATGATCAGTCCACGACTTTGGCTACTGAGAGATACTCATATCAACCAGTTCCTGTGGAGGAATCTTCTAACAAAGTTTCGCATAAATCACAAAAGAATGTGCCTGATGAGGTGTTCCTGGACAATTCTATCAGTTGTATACCTGGAATAAGTAAGAAGCGTCTCAGTCAGCTAGAAAATTGTGGCTTTCATACGGTGCGGAAATTGCTGCACCACTTTCCTCGGACCTATGCTGATTTACAGAATGCTCAAATTGACATAGATGATGGACAGTACTTGATTTTCATTGGAAAGATATTAAAATCAAGGGGAATCAAAGCTGGTACTTCTTTTTCAATTGTTGAGGTTGTCGTGGGTTGTGAAATTGCTGACAATAAAATAACCGAGCATAAGGATGATTCTACCGATTGTAGAAGGAAGACAATATATTTGCATCTGAAGAAATTTTTTCGTGGTACACGTTTTACTTCTCTACCGTTTCTTAGAATAGTTGAGAAGAAGCACAGAGAGGGAGACTTTGTATGTGTTAGTGGTAAGGTGAGGATAATGCCTGCCAAAGATCACTATGAAATGAGAGAATATAATATTGATGTCCTTAAAGATGAAAATGACTTGTCTTTTCATGCAAAAGGGAGGCCATATCCTATATACCCTTCAAAAGGAGGCTTAAATCCTGACGTTTTGAGAGATATCATTGCAAGAGTTGTACAAGTTTTACCTATCAATATTGATCCTATTCCTAAAAGAATCACTCTGGAATTTGGGCTGCTCAGTCTTCATGATGCTTATACTGAGATTCACCAACCGAAGAGTATGAGTGAAGCTGATTTGGCTCGGAAGAGGCTAATATTTGATGAGTTCTTTTACCTTCAGCTAGCGCGCTTATACCAAATGCTTGAAGGGCTTGGTACACAAATAGAGAAAGATGGATTGCTAAATAAGTATAGGAAACCTGAATCAAGTGCTGCTTATATGGAAGACTGGTCCAGTCTTACCAAGAAGTTTCTAAAAGCTCTTCCATATGCCCTTACGGGTAGTCAGCTCACTGCTGTTTCAGAAATTATCTGGGATCTCAGGCAACCAGTTCCAATGAATCGGCTTTTGCAGGGTGATGTTGGCTGTGGTAAAACTGTAGTTGCTTTTCTAGCATGCATGGAGGTAATGGGCTCTGGATACCAGGCAGCTTTCATGGTTCCTACTGAGTTGCTTGCCGTCCAGCATTACGAGCACTTGAAGAATTTGCTTGAAACTATGGAGGATGTTGAGTGCAAACCTTCTATTGCCTTACTAACAGGATCAACCCCATCAAAACAATCACGGATGATTCAAAAGAGTCTCCAGACTGGAGAGATATCAATGGTCATTGGAACGACTAGTTTAATAGCTGACCGTGTGGAGTTCTCTGCATTACGGATTGCTGTGGTGGATGAACAACATCGTTTTGGTGTCATCCAGAGAGGACGGTTTAACAGTAAGCTATACTATACCTCCATAATATCTAACATGCTAGCCACAAACTCAGATGGGACCTCCAAAGGTGAAAAGCATATGGCTCCTCATGTTCTTGCCATGTCAGCTACTCCGATCCCAAGGACGCTTGCTCTGGCTTTATATGGGGATATGTCATTAACACAAATAACAGACTTGCCACCAGGAAGAATACCAGTGGAGACATTCATTATTCAAGGAAATGAAAATGGGTATGAGGATGCATATGAGATGATGCTTGATGAGTTAAAAGAAGGAGGCAAAGTGTATCTAGTGTATCCAGTCATTGAACAATCTGAGCAGCTGCCTCAACTCCGTGCAGCTTCTGCGGATTTTGAAGCTATATCTCATAGGTTTCAGGGCTACAGCTGTGGATTGTTGCATGGAAAAATGAAGAGTGATGAGAAAGATGAAGCACTGAGAAAGTTTAGGTCTGGAGAAACAAATATACTTCTTGCCACACAAGTAATTGAGATTGGTGTTGATGTCCCAGATGCATCTATGATGGTTGTTATGAATGCTGAGAGGTTTGGGATTGCTCAGTTACATCAGCTTAGAGGACGAGTTGGACGAGGTGTGAGAAAGTCTAAGTGTATACTGTTAGCATCTTCTGAGAGTAGCCTACCACGGCTGAAGGTCCTTGGGAAATCATCAGATGGGTTTTACTTGGCGAATATGGACCTTCTTTTACGTGGACCTGGTAACTTGCTTGGCAAGAAACAGTCGGGACATCTTCCAGAGTTTCCTATTGCAAGATTGGAAATAGATGGCAATATCTTACAAGAAGCACACCTTGCTGCTCTGAAAATTTTAGGTGATTCTCATGATTTGGAGCAATTCCCGCTGCTCAAAGCAGAGCTTAGCATGAGACAGCCTCTGTCTATTCTGGGTACCATATTTTTTGTACATCAAAGGTTCACTAGCTTTGATGTTTCTGTGCAATTCTTCGTGATGCTAGCTAGCTTGATCAAGCGGGTATCTATGGCGAGCTTGGAGTTGACTATGTCTGTTCCTGGCTTCTCTTCCTACCCATCTCTTCCTTCCTCTGTGAGGAACTTGGACATAAACCAAGTACCATTGGCACAAAGACCAGATCAAGAAGAAATACTGGAGGACGAAGAAGATAGCAGCAACGGGTCGGGCGGCCCGCCCCGGAAGAAGCTCCGTCTCACCAAGGAACAGTCCCGTCTTCTCGAAGAGAGCTTCAGACAACACCACACCTTAAACCCCAGGCAGAAAGAGGCTTTGGCTTTGCAGTTGAAGCTGAGGCCACGCCAAGTTGAGGTGTGGTTTCAAAATCGTAGGGCCAGGAGCAAGCTGAAGCAAACAGAGATGGAATGCGAGTATTTGAAGAGATGGTTCGGATCACTGACTGAACAGAATAGGAGGCTGCAGAGGGAGGTCGAAGAGCTGAGGGCCATGAAAGTTGGTCCACCAACTGTGATTTCCCCCCATACGTGCGAGCCTCTTCCGGCATCCACCCTCACTATGTGCCCTCGTTGCGAGCGAGTCACCACCACGTCCACAACCACCGGCCCTACAAAGACAAAAATCTCGGCCGCTGCCAACACCGCCTTGTCCTCTAAAGTGGCGACGCCCGCCCTCTAA

Protein Analysis

1225

Amino Acids

137.16

Weight (kDa)

7.16

Isoelectric Point (pI)

47.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 540 - 722 4.7e-07 Type III restriction enzyme, res subunit
DEAD PF00270 559 - 726 5e-19 DEAD/DEAH box helicase
Helicase_C PF00271 768 - 881 1.3e-19 Helicase conserved C-terminal domain
RecG_dom3_C PF19833 908 - 965 5e-08 ATP-dependent DNA helicase RecG, domain 3, C-terminal
Homeodomain PF00046 1075 - 1129 1.6e-14 Homeodomain
HALZ PF02183 1131 - 1165 4.1e-09 Homeobox associated leucine zipper
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 2960
AccB1I GGYRCC 1 cut(s) 2960
AccI GTMKAC 2 cut(s) 721, 2665
AccII CGCG 1 cut(s) 1462
AciI CCGC 9 cut(s) 781, 2399, 2912, 3043, 3213, 3218, 3630, 3642, 3669
AclWI GGATC 5 cut(s) 1307, 1659, 1885, 2161, 3442
AcoI YGGCCR 1 cut(s) 3627
AcsI RAATTY 5 cut(s) 322, 1025, 1340, 1819, 2877
AcuI CTGAAG 6 cut(s) 492, 1040, 1436, 2723, 3259, 3410
AcyI GRCGYC 1 cut(s) 3665
AdeI CACNNNGTG 1 cut(s) 3561
AfaI GTAC 9 cut(s) 118, 857, 907, 1039, 1290, 1492, 2962, 2976, 3144
AfiI CCNNNNNNNGG 4 cut(s) 406, 1241, 3120, 3352
AflIII ACRYGT 1 cut(s) 1040
AhlI ACTAGT 1 cut(s) 1955
AjiI CACGTC 1 cut(s) 3591
AjnI CCWGG 8 cut(s) 699, 724, 1758, 2233, 2767, 3082, 3299, 3377
AjuI GAANNNNNNNTTGG 4 cut(s) 3299, 3331, 3499, 3531
Alw21I GWGCWC 1 cut(s) 1812
Alw26I GTCTC 6 cut(s) 749, 1089, 1922, 2246, 2932, 3242
AlwI GGATC 5 cut(s) 1307, 1659, 1885, 2161, 3442
AlwNI CAGNNNCTG 3 cut(s) 635, 2396, 2947
AoxI GGCC 7 cut(s) 1217, 3214, 3338, 3375, 3486, 3604, 3627
ApoI RAATTY 5 cut(s) 322, 1025, 1340, 1819, 2877
ArsI GACNNNNNNTTYG 2 cut(s) 994, 1026
Asp700I GAANNNNTTC 1 cut(s) 477
Asp718I GGTACC 1 cut(s) 2960
AspLEI GCGC 2 cut(s) 1462, 1464
AsuC2I CCSGG 1 cut(s) 3223
AsuHPI GGTGA 8 cut(s) 404, 1132, 1373, 1703, 2132, 2897, 3232, 3574
AsuNHI GCTAGC 4 cut(s) 1456, 2089, 3025, 3029
AvaII GGWCC 7 cut(s) 807, 1566, 2109, 2707, 2749, 2765, 3500
BaeGI GKGCMC 1 cut(s) 3566
BanI GGYRCC 1 cut(s) 2960
BanII GRGCYC 1 cut(s) 1751
BbsI GAAGAC 5 cut(s) 266, 1007, 1349, 1566, 3251
Bbv12I GWGCWC 1 cut(s) 1812
BbvCI CCTCAGC 2 cut(s) 3335, 3482
BceAI ACGGC 2 cut(s) 1778, 2714
BcgI CGANNNNNNTGC 2 cut(s) 13, 47
BciT130I CCWGG 8 cut(s) 701, 726, 1760, 2235, 2769, 3084, 3301, 3379
BciVI GTATCC 3 cut(s) 555, 1748, 2358
BclI TGATCA 2 cut(s) 586, 3036
BcnI CCSGG 1 cut(s) 3223
BcoDI GTCTC 6 cut(s) 749, 1089, 1922, 2246, 2932, 3242
BcuI ACTAGT 1 cut(s) 1955
BfaI CTAG 9 cut(s) 755, 1457, 1727, 1956, 2090, 2342, 2991, 3026, 3030
BfmI CTRYAG 3 cut(s) 1713, 2434, 3461
BfuI GTATCC 3 cut(s) 555, 1748, 2358
BlpI GCTNAGC 1 cut(s) 2930
BmcAI AGTACT 2 cut(s) 118, 857
Bme1390I CCNGG 9 cut(s) 701, 726, 1760, 2235, 2769, 3084, 3223, 3301, 3379
Bme18I GGWCC 7 cut(s) 807, 1566, 2109, 2707, 2749, 2765, 3500
BmgBI CACGTC 1 cut(s) 3591
BmiI GGNNCC 4 cut(s) 1776, 2110, 2136, 2962
BmrFI CCNGG 9 cut(s) 701, 726, 1760, 2235, 2769, 3084, 3223, 3301, 3379
BmtI GCTAGC 4 cut(s) 1460, 2093, 3029, 3033
BpiI GAAGAC 5 cut(s) 266, 1007, 1349, 1566, 3251
BplI GAGNNNNNCTC 6 cut(s) 504, 536, 602, 634, 2929, 2961
BpmI CTGGAG 4 cut(s) 1904, 1947, 2526, 3200
Bpu10I CCTNAGC 2 cut(s) 3335, 3482
Bpu1102I GCTNAGC 1 cut(s) 2930
BpuMI CCSGG 1 cut(s) 3223
BsaAI YACGTR 2 cut(s) 2762, 3528
BsaBI GATNNNNATC 1 cut(s) 376
BsaHI GRCGYC 1 cut(s) 3665
BsaJI CCNNGG 7 cut(s) 803, 2171, 2696, 2710, 3221, 3243, 3299
BsaWI WCCGGW 1 cut(s) 403
BsaXI ACNNNNNCTCC 6 cut(s) 1086, 1116, 2054, 2084, 2319, 2349
Bsc4I CCNNNNNNNGG 4 cut(s) 406, 1241, 3120, 3352
Bse118I RCCGGY 1 cut(s) 3602
Bse1I ACTGG 9 cut(s) 12, 629, 1568, 1569, 1664, 1930, 2246, 2351, 3183
Bse3DI GCAATG 2 cut(s) 342, 1275
Bse8I GATNNNNATC 1 cut(s) 376
BseBI CCWGG 8 cut(s) 701, 726, 1760, 2235, 2769, 3084, 3301, 3379
BseDI CCNNGG 7 cut(s) 803, 2171, 2696, 2710, 3221, 3243, 3299
BseGI GGATG 8 cut(s) 346, 982, 1846, 1908, 2014, 2031, 2296, 3545
BseJI GATNNNNATC 1 cut(s) 376
BseLI CCNNNNNNNGG 4 cut(s) 406, 1241, 3120, 3352
BseMI GCAATG 2 cut(s) 342, 1275
BseNI ACTGG 9 cut(s) 12, 629, 1568, 1569, 1664, 1930, 2246, 2351, 3183
BsePI GCGCGC 1 cut(s) 1460
BseRI GAGGAG 2 cut(s) 203, 2127
BseSI GKGCMC 1 cut(s) 3566
BseX3I CGGCCG 1 cut(s) 3627
BsgI GTGCAG 2 cut(s) 776, 2409
Bsh1236I CGCG 1 cut(s) 1462
Bsh1285I CGRYCG 1 cut(s) 3630
BshFI GGCC 7 cut(s) 1219, 3216, 3340, 3377, 3488, 3606, 3629
BshNI GGYRCC 1 cut(s) 2960
BsiEI CGRYCG 1 cut(s) 3630
BsiHKAI GWGCWC 1 cut(s) 1812
BsiSI CCGG 4 cut(s) 404, 3223, 3542, 3603
BslFI GGGAC 5 cut(s) 436, 2122, 2543, 2811, 3238
BslI CCNNNNNNNGG 4 cut(s) 406, 1241, 3120, 3352
BsmAI GTCTC 6 cut(s) 749, 1089, 1922, 2246, 2932, 3242
BsmBI CGTCTC 2 cut(s) 749, 3242
BsmFI GGGAC 5 cut(s) 436, 2122, 2543, 2811, 3238
BsmI GAATGC 4 cut(s) 94, 832, 2593, 3413
BsnI GGCC 7 cut(s) 1219, 3216, 3340, 3377, 3488, 3606, 3629
Bsp1286I GDGCHC 3 cut(s) 1751, 1812, 3566
Bsp1407I TGTACA 2 cut(s) 1288, 2974
Bsp1720I GCTNAGC 1 cut(s) 2930
BspACI CCGC 9 cut(s) 781, 2399, 2912, 3043, 3213, 3218, 3630, 3642, 3669
BspANI GGCC 7 cut(s) 1219, 3216, 3340, 3377, 3488, 3606, 3629
BspFNI CGCG 1 cut(s) 1462
BspHI TCATGA 3 cut(s) 277, 1360, 2893
BspLI GGNNCC 4 cut(s) 1776, 2110, 2136, 2962
BspMAI CTGCAG 1 cut(s) 3465
BspOI GCTAGC 4 cut(s) 1460, 2093, 3029, 3033
BspPI GGATC 5 cut(s) 1307, 1659, 1885, 2161, 3442
BspQI GCTCTTC 3 cut(s) 495, 1602, 3471
BspT107I GGYRCC 1 cut(s) 2960
BsrDI GCAATG 2 cut(s) 342, 1275
BsrFI RCCGGY 1 cut(s) 3602
BsrGI TGTACA 2 cut(s) 1288, 2974
BsrI ACTGG 9 cut(s) 12, 629, 1568, 1569, 1664, 1930, 2246, 2351, 3183
BssAI RCCGGY 1 cut(s) 3602
BssECI CCNNGG 7 cut(s) 803, 2171, 2696, 2710, 3221, 3243, 3299
BssHII GCGCGC 1 cut(s) 1460
BssNAI GTATAC 2 cut(s) 722, 2666
BssNI GRCGYC 1 cut(s) 3665
BssT1I CCWWGG 3 cut(s) 2171, 2710, 3243
Bst1107I GTATAC 2 cut(s) 722, 2666
Bst2UI CCWGG 8 cut(s) 701, 726, 1760, 2235, 2769, 3084, 3301, 3379
BstACI GRCGYC 1 cut(s) 3665
BstAPI GCANNNNNTGC 2 cut(s) 2396, 2864
BstAUI TGTACA 2 cut(s) 1288, 2974
BstBAI YACGTR 2 cut(s) 2762, 3528
BstDSI CCRYGG 1 cut(s) 2696
BstENI CCTNNNNNAGG 2 cut(s) 1239, 3118
BstF5I GGATG 8 cut(s) 346, 982, 1846, 1908, 2014, 2031, 2296, 3545
BstFNI CGCG 1 cut(s) 1462
BstHHI GCGC 2 cut(s) 1462, 1464
BstMAI GTCTC 6 cut(s) 749, 1089, 1922, 2246, 2932, 3242
BstMCI CGRYCG 1 cut(s) 3630
BstNI CCWGG 8 cut(s) 701, 726, 1760, 2235, 2769, 3084, 3301, 3379
BstNSI RCATGY 3 cut(s) 28, 1734, 2089
BstSCI CCNGG 9 cut(s) 699, 724, 1758, 2233, 2767, 3082, 3221, 3299, 3377
BstSFI CTRYAG 3 cut(s) 1713, 2434, 3461
BstSLI GKGCMC 1 cut(s) 3566
BstUI CGCG 1 cut(s) 1462
BstV2I GAAGAC 5 cut(s) 266, 1007, 1349, 1566, 3251
BstX2I RGATCY 1 cut(s) 1651
BstXI CCANNNNNNTGG 1 cut(s) 601
BstYI RGATCY 1 cut(s) 1651
BstZ17I GTATAC 2 cut(s) 722, 2666
BstZI CGGCCG 1 cut(s) 3627
BsuI GTATCC 3 cut(s) 555, 1748, 2358
BsuRI GGCC 7 cut(s) 1219, 3216, 3340, 3377, 3488, 3606, 3629
BtgI CCRYGG 1 cut(s) 2696
BtrI CACGTC 1 cut(s) 3591
BtsCI GGATG 8 cut(s) 346, 982, 1846, 1908, 2014, 2031, 2296, 3545
BtsI GCAGTG 1 cut(s) 1626
BtsIMutI CAGTG 5 cut(s) 5, 1626, 2253, 2486, 3437
CaiI CAGNNNCTG 3 cut(s) 635, 2396, 2947
CciI TCATGA 3 cut(s) 277, 1360, 2893
CfoI GCGC 2 cut(s) 1462, 1464
Cfr10I RCCGGY 1 cut(s) 3602
CseI GACGC 3 cut(s) 731, 2185, 3673
CsiI ACCWGGT 1 cut(s) 2767
Csp6I GTAC 9 cut(s) 117, 856, 906, 1038, 1289, 1491, 2961, 2975, 3143
CviQI GTAC 9 cut(s) 117, 856, 906, 1038, 1289, 1491, 2961, 2975, 3143
DraI TTTAAA 1 cut(s) 328
DraIII CACNNNGTG 1 cut(s) 3561
EaeI YGGCCR 1 cut(s) 3627
EagI CGGCCG 1 cut(s) 3627
EclXI CGGCCG 1 cut(s) 3627
Eco130I CCWWGG 3 cut(s) 2171, 2710, 3243
Eco24I GRGCYC 1 cut(s) 1751
Eco32I GATATC 3 cut(s) 83, 1273, 1935
Eco47I GGWCC 7 cut(s) 807, 1566, 2109, 2707, 2749, 2765, 3500
Eco52I CGGCCG 1 cut(s) 3627
Eco57I CTGAAG 6 cut(s) 492, 1040, 1436, 2723, 3259, 3410
EcoNI CCTNNNNNAGG 2 cut(s) 1239, 3118
EcoO109I RGGNCCY 2 cut(s) 2109, 2707
EcoRII CCWGG 8 cut(s) 699, 724, 1758, 2233, 2767, 3082, 3299, 3377
EcoRV GATATC 3 cut(s) 83, 1273, 1935
EcoT14I CCWWGG 3 cut(s) 2171, 2710, 3243
EcoT22I ATGCAT 4 cut(s) 94, 1736, 2296, 2569
EcoT38I GRGCYC 1 cut(s) 1751
ErhI CCWWGG 3 cut(s) 2171, 2710, 3243
Esp3I CGTCTC 2 cut(s) 749, 3242
FalI AAGNNNNNCTT 8 cut(s) 152, 184, 1534, 1566, 2847, 2879, 2913, 2945
FaqI GGGAC 5 cut(s) 436, 2122, 2543, 2811, 3238
FauI CCCGC 4 cut(s) 2919, 3036, 3225, 3676
FauNDI CATATG 3 cut(s) 1603, 2130, 2296
FbaI TGATCA 2 cut(s) 586, 3036
FblI GTMKAC 2 cut(s) 721, 2665
FokI GGATG 8 cut(s) 353, 989, 1853, 1915, 2018, 2021, 2303, 3532
FriOI GRGCYC 1 cut(s) 1751
FspBI CTAG 9 cut(s) 755, 1457, 1727, 1956, 2090, 2342, 2991, 3026, 3030
GlaI GCGC 2 cut(s) 1461, 1463
GsuI CTGGAG 4 cut(s) 1904, 1947, 2526, 3200
HaeIII GGCC 7 cut(s) 1219, 3216, 3340, 3377, 3488, 3606, 3629
HapII CCGG 4 cut(s) 404, 3223, 3542, 3603
HgaI GACGC 3 cut(s) 731, 2185, 3673
HhaI GCGC 2 cut(s) 1462, 1464
Hin1I GRCGYC 1 cut(s) 3665
Hin6I GCGC 2 cut(s) 1460, 1462
HinP1I GCGC 2 cut(s) 1460, 1462
HincII GTYRAC 2 cut(s) 579, 3069
HindII GTYRAC 2 cut(s) 579, 3069
HpaI GTTAAC 1 cut(s) 579
HpaII CCGG 4 cut(s) 404, 3223, 3542, 3603
HphI GGTGA 8 cut(s) 404, 1132, 1373, 1703, 2132, 2897, 3232, 3574
Hpy99I CGWCG 1 cut(s) 3667
HpyCH4IV ACGT 5 cut(s) 1042, 1260, 2761, 3527, 3590
HpySE526I ACGT 5 cut(s) 1042, 1260, 2761, 3527, 3590
Hsp92I GRCGYC 1 cut(s) 3665
HspAI GCGC 2 cut(s) 1460, 1462
KpnI GGTACC 1 cut(s) 2964
Ksp22I TGATCA 2 cut(s) 586, 3036
KspAI GTTAAC 1 cut(s) 579
LguI GCTCTTC 3 cut(s) 495, 1602, 3471
LmnI GCTCC 4 cut(s) 2140, 2902, 3237, 3381
MabI ACCWGGT 1 cut(s) 2767
MaeI CTAG 9 cut(s) 755, 1457, 1727, 1956, 2090, 2342, 2991, 3026, 3030
MaeII ACGT 5 cut(s) 1042, 1260, 2761, 3527, 3590
MaeIII GTNAC 3 cut(s) 2613, 2771, 3580
MfeI CAATTG 1 cut(s) 918
MflI RGATCY 1 cut(s) 1651
MhlI GDGCHC 3 cut(s) 1751, 1812, 3566
MlyI GAGTC 2 cut(s) 1924, 3588
MmeI TCCRAC 1 cut(s) 2617
Mph1103I ATGCAT 4 cut(s) 94, 1736, 2296, 2569
MroXI GAANNNNTTC 1 cut(s) 477
MslI CAYNNNNRTG 1 cut(s) 975
MspA1I CMGCKG 5 cut(s) 20, 2374, 2439, 2914, 3632
MspI CCGG 4 cut(s) 404, 3223, 3542, 3603
MspR9I CCNGG 9 cut(s) 701, 726, 1760, 2235, 2769, 3084, 3223, 3301, 3379
MunI CAATTG 1 cut(s) 918
Mva1269I GAATGC 4 cut(s) 94, 832, 2593, 3413
MvaI CCWGG 8 cut(s) 701, 726, 1760, 2235, 2769, 3084, 3301, 3379
MvnI CGCG 1 cut(s) 1462
NciI CCSGG 1 cut(s) 3223
NdeI CATATG 3 cut(s) 1603, 2130, 2296
NheI GCTAGC 4 cut(s) 1456, 2089, 3025, 3029
NlaIV GGNNCC 4 cut(s) 1776, 2110, 2136, 2962
NmeAIII GCCGAG 1 cut(s) 3605
NmuCI GTSAC 1 cut(s) 3580
NsiI ATGCAT 4 cut(s) 94, 1736, 2296, 2569
NspI RCATGY 3 cut(s) 28, 1734, 2089
PaeI GCATGC 1 cut(s) 1734
PagI TCATGA 3 cut(s) 277, 1360, 2893
PauI GCGCGC 1 cut(s) 1460
PciSI GCTCTTC 3 cut(s) 495, 1602, 3471
PcsI WCGNNNNNNNCGW 3 cut(s) 1039, 2638, 3574
PctI GAATGC 4 cut(s) 94, 832, 2593, 3413
PdmI GAANNNNTTC 1 cut(s) 477
PflFI GACNNNGTC 1 cut(s) 3073
PfoI TCCNGGA 1 cut(s) 699
PleI GAGTC 2 cut(s) 1923, 3587
PpsI GAGTC 2 cut(s) 1923, 3587
Ppu21I YACGTR 2 cut(s) 2762, 3528
PpuMI RGGWCCY 2 cut(s) 2109, 2707
Psp5II RGGWCCY 2 cut(s) 2109, 2707
Psp6I CCWGG 8 cut(s) 699, 724, 1758, 2233, 2767, 3082, 3299, 3377
PspGI CCWGG 8 cut(s) 699, 724, 1758, 2233, 2767, 3082, 3299, 3377
PspN4I GGNNCC 4 cut(s) 1776, 2110, 2136, 2962
PspPPI RGGWCCY 2 cut(s) 2109, 2707
PstI CTGCAG 1 cut(s) 3465
PstNI CAGNNNCTG 3 cut(s) 635, 2396, 2947
PsuI RGATCY 1 cut(s) 1651
PsyI GACNNNGTC 1 cut(s) 3073
PteI GCGCGC 1 cut(s) 1460
PvuII CAGCTG 3 cut(s) 20, 2374, 2439
RsaI GTAC 9 cut(s) 118, 857, 907, 1039, 1290, 1492, 2962, 2976, 3144
RsaNI GTAC 9 cut(s) 117, 856, 906, 1038, 1289, 1491, 2961, 2975, 3143
RseI CAYNNNNRTG 1 cut(s) 975
SapI GCTCTTC 3 cut(s) 495, 1602, 3471
ScaI AGTACT 2 cut(s) 118, 857
SchI GAGTC 2 cut(s) 1924, 3588
ScrFI CCNGG 9 cut(s) 701, 726, 1760, 2235, 2769, 3084, 3223, 3301, 3379
SduI GDGCHC 3 cut(s) 1751, 1812, 3566
SexAI ACCWGGT 1 cut(s) 2767
SfcI CTRYAG 3 cut(s) 1713, 2434, 3461
SinI GGWCC 7 cut(s) 807, 1566, 2109, 2707, 2749, 2765, 3500
SmiMI CAYNNNNRTG 1 cut(s) 975
SpeI ACTAGT 1 cut(s) 1955
SphI GCATGC 1 cut(s) 1734
SsiI CCGC 9 cut(s) 781, 2399, 2912, 3043, 3213, 3218, 3630, 3642, 3669
SspI AATATT 3 cut(s) 1168, 1309, 1431
SspMI CTAG 9 cut(s) 755, 1457, 1727, 1956, 2090, 2342, 2991, 3026, 3030
StyD4I CCNGG 9 cut(s) 699, 724, 1758, 2233, 2767, 3082, 3221, 3299, 3377
StyI CCWWGG 3 cut(s) 2171, 2710, 3243
TaiI ACGT 5 cut(s) 1045, 1263, 2764, 3530, 3593
TaqI TCGA 3 cut(s) 154, 3264, 3474
TaqII GACCGA 1 cut(s) 216
TatI WGTACW 4 cut(s) 116, 855, 1288, 2974
TauI GCSGC 2 cut(s) 3216, 3632
TscAI CASTG 5 cut(s) 12, 1633, 2253, 2493, 3444
TseFI GTSAC 1 cut(s) 3580
Tsp45I GTSAC 1 cut(s) 3580
TspGWI ACGGA 4 cut(s) 1915, 2008, 2375, 3224
TspRI CASTG 5 cut(s) 12, 1633, 2253, 2493, 3444
Tth111I GACNNNGTC 1 cut(s) 3073
VpaK11BI GGWCC 7 cut(s) 807, 1566, 2109, 2707, 2749, 2765, 3500
XagI CCTNNNNNAGG 2 cut(s) 1239, 3118
XapI RAATTY 5 cut(s) 322, 1025, 1340, 1819, 2877
XceI RCATGY 3 cut(s) 28, 1734, 2089
XmiI GTMKAC 2 cut(s) 721, 2665
XmnI GAANNNNTTC 1 cut(s) 477
XspI CTAG 9 cut(s) 755, 1457, 1727, 1956, 2090, 2342, 2991, 3026, 3030
ZrmI AGTACT 2 cut(s) 118, 857
Zsp2I ATGCAT 4 cut(s) 94, 1736, 2296, 2569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.