Rh5BG040200

Anthocyanidin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0010753
Physical Location & Seq
Forward (+)
2901914 .. 2902953
1040 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG040200.1

Sequence Viewer

Length: 549 bp
ATGGCCACCCCCCAACCCATCGTCTCAAACAAGACTGCTTGTGTGATCGGCGGCACCGGCTTCGTGGCGTCTCTGCTGGTCAAGCTCTTGCTAGAGAAGGGCTATGTCGTCAAAACCACTGCTAGAGACCCTGACAATCTGAAGAAGATCTCCCACCTCACAGCACTACAAGAGTTGGGAAAGCTGACTATTTTTCGTGGTGATTTAACCGATGAAGGGAGCTTCGATGCTGCTATAGCAGGTTCTGATCTTGTTTTCCATGTTGCCACACCAGTCAACTTTGGCTCACCGGACCCGGAGAATGACATGATCAAGCCAGGAGTCCAAGGAGTACTAAATGTTCTGAAATCATGTGTGAAAGCAAAAACAGTTAAGCGAGTTGTTTTAACATCATCAGCAGCTGCAGTAACTGTCAATACTCTTAGTGGCACAGGCTTGATTGCGGACGAAAATGATTGGTCTGATGTTGAGTTCTTGACCACTGCCAAGCCACCTACTTGGGTAAATCACCAAATTTTTTTAGTTTATAAGAGTAATTGTCTTGTGTAA

Protein Analysis

182

Amino Acids

19.37

Weight (kDa)

6.82

Isoelectric Point (pI)

17.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 10 - 129 1.5e-10 NmrA-like family
Epimerase PF01370 14 - 135 3.5e-15 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 15 - 151 2.8e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 15 - 133 7e-11 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 17 - 136 1.7e-13 NAD(P)H-binding
NAD_binding_4 PF07993 17 - 137 1.1e-07 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 528
Acc36I ACCTGC 1 cut(s) 230
AccB1I GGYRCC 1 cut(s) 53
AciI CCGC 2 cut(s) 51, 443
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 513
AcuI CTGAAG 1 cut(s) 161
AcyI GRCGYC 1 cut(s) 68
AfaI GTAC 1 cut(s) 333
AfiI CCNNNNNNNGG 1 cut(s) 216
AjnI CCWGG 1 cut(s) 316
AluBI AGCT 4 cut(s) 85, 184, 222, 401
AluI AGCT 4 cut(s) 85, 184, 222, 401
Alw26I GTCTC 3 cut(s) 28, 75, 120
AlwNI CAGNNNCTG 3 cut(s) 245, 401, 410
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 3 cut(s) 230, 398, 401
ApoI RAATTY 1 cut(s) 513
AspS9I GGNCC 1 cut(s) 292
AsuC2I CCSGG 1 cut(s) 296
AsuHPI GGTGA 3 cut(s) 212, 279, 500
AvaII GGWCC 1 cut(s) 292
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 53
BbvI GCAGC 3 cut(s) 217, 388, 410
BccI CCATC 1 cut(s) 26
BcgI CGANNNNNNTGC 2 cut(s) 43, 77
BciT130I CCWGG 1 cut(s) 318
BclI TGATCA 1 cut(s) 309
BcnI CCSGG 1 cut(s) 296
BcoDI GTCTC 3 cut(s) 28, 75, 120
BfaI CTAG 2 cut(s) 92, 123
BfmI CTRYAG 2 cut(s) 234, 402
BfuAI ACCTGC 1 cut(s) 230
BglII AGATCT 1 cut(s) 147
BisI GCNGC 4 cut(s) 52, 231, 399, 402
BlsI GCNGC 4 cut(s) 53, 232, 400, 403
BmcAI AGTACT 1 cut(s) 333
Bme1390I CCNGG 2 cut(s) 296, 318
Bme18I GGWCC 1 cut(s) 292
BmgT120I GGNCC 1 cut(s) 292
BmiI GGNNCC 2 cut(s) 55, 294
BmrFI CCNGG 2 cut(s) 296, 318
BmsI GCATC 1 cut(s) 217
BpuMI CCSGG 1 cut(s) 296
BsaHI GRCGYC 1 cut(s) 68
BsaI GGTCTC 1 cut(s) 120
BsaJI CCNNGG 1 cut(s) 325
BsaWI WCCGGW 1 cut(s) 289
Bsc4I CCNNNNNNNGG 1 cut(s) 216
Bse118I RCCGGY 1 cut(s) 56
Bse1I ACTGG 1 cut(s) 272
BseBI CCWGG 1 cut(s) 318
BseDI CCNNGG 1 cut(s) 325
BseLI CCNNNNNNNGG 1 cut(s) 216
BseNI ACTGG 1 cut(s) 272
BseXI GCAGC 3 cut(s) 217, 388, 410
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 53
BsiSI CCGG 3 cut(s) 57, 290, 296
BslI CCNNNNNNNGG 1 cut(s) 216
BsmAI GTCTC 3 cut(s) 28, 75, 120
BsmBI CGTCTC 2 cut(s) 28, 75
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 120
Bsp143I GATC 4 cut(s) 45, 147, 247, 309
BspACI CCGC 2 cut(s) 51, 443
BspANI GGCC 1 cut(s) 5
BspLI GGNNCC 2 cut(s) 55, 294
BspMAI CTGCAG 1 cut(s) 406
BspMI ACCTGC 1 cut(s) 230
BspT107I GGYRCC 1 cut(s) 53
BspTNI GGTCTC 1 cut(s) 120
BsrFI RCCGGY 1 cut(s) 56
BsrI ACTGG 1 cut(s) 272
BssAI RCCGGY 1 cut(s) 56
BssECI CCNNGG 1 cut(s) 325
BssMI GATC 4 cut(s) 45, 147, 247, 309
BssNI GRCGYC 1 cut(s) 68
BssT1I CCWWGG 1 cut(s) 325
Bst2UI CCWGG 1 cut(s) 318
Bst4CI ACNGT 2 cut(s) 370, 412
BstACI GRCGYC 1 cut(s) 68
BstDEI CTNAG 1 cut(s) 422
BstKTI GATC 4 cut(s) 48, 150, 250, 312
BstMAI GTCTC 3 cut(s) 28, 75, 120
BstMBI GATC 4 cut(s) 45, 147, 247, 309
BstMWI GCNNNNNNNGC 3 cut(s) 57, 82, 236
BstNI CCWGG 1 cut(s) 318
BstSCI CCNGG 2 cut(s) 294, 316
BstSFI CTRYAG 2 cut(s) 234, 402
BstV1I GCAGC 3 cut(s) 217, 388, 410
BstX2I RGATCY 1 cut(s) 147
BstXI CCANNNNNNTGG 1 cut(s) 498
BstYI RGATCY 1 cut(s) 147
BsuRI GGCC 1 cut(s) 5
BtsI GCAGTG 2 cut(s) 117, 480
BtsIMutI CAGTG 2 cut(s) 117, 480
BveI ACCTGC 1 cut(s) 230
CaiI CAGNNNCTG 3 cut(s) 245, 401, 410
Cfr10I RCCGGY 1 cut(s) 56
Cfr13I GGNCC 1 cut(s) 292
CseI GACGC 1 cut(s) 57
Csp6I GTAC 1 cut(s) 332
CviAII CATG 3 cut(s) 260, 307, 351
CviQI GTAC 1 cut(s) 332
DdeI CTNAG 1 cut(s) 422
DpnI GATC 4 cut(s) 47, 149, 249, 311
DpnII GATC 4 cut(s) 45, 147, 247, 309
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 325
Eco31I GGTCTC 1 cut(s) 120
Eco47I GGWCC 1 cut(s) 292
Eco57I CTGAAG 1 cut(s) 161
EcoRII CCWGG 1 cut(s) 316
EcoT14I CCWWGG 1 cut(s) 325
ErhI CCWWGG 1 cut(s) 325
Esp3I CGTCTC 2 cut(s) 28, 75
FaeI CATG 3 cut(s) 263, 310, 354
FaiI YATR 6 cut(s) 105, 236, 261, 308, 352, 528
FatI CATG 3 cut(s) 259, 306, 350
FbaI TGATCA 1 cut(s) 309
Fnu4HI GCNGC 4 cut(s) 52, 231, 399, 402
Fsp4HI GCNGC 4 cut(s) 52, 231, 399, 402
FspBI CTAG 2 cut(s) 92, 123
GluI GCNGC 4 cut(s) 52, 231, 399, 402
HaeIII GGCC 1 cut(s) 5
HapII CCGG 3 cut(s) 57, 290, 296
HgaI GACGC 1 cut(s) 57
Hin1I GRCGYC 1 cut(s) 68
Hin1II CATG 3 cut(s) 263, 310, 354
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 1 cut(s) 321
HpaII CCGG 3 cut(s) 57, 290, 296
HphI GGTGA 3 cut(s) 212, 279, 500
Hpy166II GTNNAC 1 cut(s) 277
Hpy188I TCNGA 4 cut(s) 141, 247, 345, 463
Hpy188III TCNNGA 1 cut(s) 475
Hpy8I GTNNAC 1 cut(s) 277
HpyAV CCTTC 2 cut(s) 91, 209
HpyCH4III ACNGT 2 cut(s) 370, 412
HpyCH4V TGCA 1 cut(s) 404
HpyF10VI GCNNNNNNNGC 3 cut(s) 57, 82, 236
HpyF3I CTNAG 1 cut(s) 422
Hsp92I GRCGYC 1 cut(s) 68
Hsp92II CATG 3 cut(s) 263, 310, 354
Ksp22I TGATCA 1 cut(s) 309
Kzo9I GATC 4 cut(s) 45, 147, 247, 309
LmnI GCTCC 1 cut(s) 219
Lsp1109I GCAGC 3 cut(s) 217, 388, 410
LweI GCATC 1 cut(s) 217
MaeI CTAG 2 cut(s) 92, 123
MaeIII GTNAC 1 cut(s) 406
MalI GATC 4 cut(s) 47, 149, 249, 311
MboI GATC 4 cut(s) 45, 147, 247, 309
MboII GAAGA 2 cut(s) 154, 157
MflI RGATCY 1 cut(s) 147
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 513, 535
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 330
MnlI CCTC 1 cut(s) 167
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 206, 372, 386
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 401
MspI CCGG 3 cut(s) 57, 290, 296
MspR9I CCNGG 2 cut(s) 296, 318
MvaI CCWGG 1 cut(s) 318
MwoI GCNNNNNNNGC 3 cut(s) 57, 82, 236
NciI CCSGG 1 cut(s) 296
NdeII GATC 4 cut(s) 45, 147, 247, 309
NlaIII CATG 3 cut(s) 263, 310, 354
NlaIV GGNNCC 2 cut(s) 55, 294
PkrI GCNGC 4 cut(s) 53, 232, 400, 403
PleI GAGTC 1 cut(s) 329
PpsI GAGTC 1 cut(s) 329
PsiI TTATAA 1 cut(s) 528
Psp6I CCWGG 1 cut(s) 316
PspGI CCWGG 1 cut(s) 316
PspN4I GGNNCC 2 cut(s) 55, 294
PspPI GGNCC 1 cut(s) 292
PsrI GAACNNNNNNTAC 2 cut(s) 324, 356
PstI CTGCAG 1 cut(s) 406
PstNI CAGNNNCTG 3 cut(s) 245, 401, 410
PsuI RGATCY 1 cut(s) 147
PvuII CAGCTG 1 cut(s) 401
RsaI GTAC 1 cut(s) 333
RsaNI GTAC 1 cut(s) 332
SaqAI TTAA 3 cut(s) 206, 372, 386
SatI GCNGC 4 cut(s) 52, 231, 399, 402
Sau3AI GATC 4 cut(s) 45, 147, 247, 309
Sau96I GGNCC 1 cut(s) 292
ScaI AGTACT 1 cut(s) 333
SchI GAGTC 1 cut(s) 330
ScrFI CCNGG 2 cut(s) 296, 318
SetI ASST 7 cut(s) 87, 159, 186, 224, 244, 403, 496
SfaNI GCATC 1 cut(s) 217
SfcI CTRYAG 2 cut(s) 234, 402
SinI GGWCC 1 cut(s) 292
Sse9I AATT 2 cut(s) 513, 535
SsiI CCGC 2 cut(s) 51, 443
SspMI CTAG 2 cut(s) 92, 123
StyD4I CCNGG 2 cut(s) 294, 316
StyI CCWWGG 1 cut(s) 325
TaaI ACNGT 2 cut(s) 370, 412
TaqI TCGA 1 cut(s) 225
TasI AATT 2 cut(s) 513, 535
TatI WGTACW 1 cut(s) 331
TauI GCSGC 1 cut(s) 54
Tru1I TTAA 3 cut(s) 206, 372, 386
Tru9I TTAA 3 cut(s) 206, 372, 386
TscAI CASTG 2 cut(s) 124, 487
TseI GCWGC 3 cut(s) 230, 398, 401
TspDTI ATGAA 1 cut(s) 228
TspRI CASTG 2 cut(s) 124, 487
VpaK11BI GGWCC 1 cut(s) 292
XapI RAATTY 1 cut(s) 513
XspI CTAG 2 cut(s) 92, 123
ZrmI AGTACT 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.