Rroxscaffold_1G00070930

Anthocyanidin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0010753
Physical Location & Seq
Reverse (-)
91903508 .. 91905816
2309 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00070930.1

Sequence Viewer

Length: 714 bp
ATGATCAAGCCAGGAGTCCAAGGGGTACTAAATGTTCTGAAATCATGTGTGAAAGCAAAAACAGTTAAGCGAGTTGTTTTAACATCATCAGCAGCTGCAGTAACTGTCAATACTCTTAGTGGCACAGGCTTGATTGCGGACGAAAATGATTGGTCTGATGTTGAGTTCTTGACCACTGCCAAGCCACCTACTTGGGGGTATCCTGTTTCCAAGGTACTAGCTGAGAAGACAGCTTGGAAATTTGCTGAAGAAAACAACATTGATCTCATCACTGTGATCCCTTCTCTCATGGCTGGTGCTTCTCTCACTCCAGACATCCCCAGCAGTATAGGCCTCGCCACATCTTTAATTACAGGAAATGAATTCCTTATAAATGGCTTGAAAGGCATGCAAATGCTATCAGGTTCCATATCCATTACACATGTGGAGGATGTCTGCCGTGCTCATATCTTTTTGGCAGAGAAAGAATCTGCTTCCGGTCGGTACATATGCTGTGCTGAAAATAGCAGTGTTCCCGAGGTTGCAAAGTTCCTCAGCAAAAGATATCCCGGCTACAAAGTCCCGACTGAGTTTGGAGATTTTCCATCCAAGGCCAAGACCATACTCTCTTCGGAAAAGCTTAAGAAGGAGGGGTTCACTTTCAAGTATGGGATTGAAGACATTTATGACCAAGCTGTGGAGTACTTGAAAGTTAAGGGGGTGCTGCAGAGCTAG

Protein Analysis

237

Amino Acids

25.61

Weight (kDa)

7.59

Isoelectric Point (pI)

28.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 5 - 158 8.5e-11 NAD dependent epimerase/dehydratase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 371
AccB7I CCANNNNNTGG 1 cut(s) 676
AciI CCGC 1 cut(s) 137
AclWI GGATC 1 cut(s) 271
AcsI RAATTY 2 cut(s) 239, 362
AcuI CTGAAG 1 cut(s) 267
AfaI GTAC 4 cut(s) 27, 216, 485, 683
AfiI CCNNNNNNNGG 2 cut(s) 194, 676
AflII CTTAAG 1 cut(s) 620
AflIII ACRYGT 1 cut(s) 421
AgsI TTSAA 4 cut(s) 382, 643, 656, 688
AjnI CCWGG 1 cut(s) 10
AluBI AGCT 6 cut(s) 95, 221, 233, 619, 674, 711
AluI AGCT 6 cut(s) 95, 221, 233, 619, 674, 711
Alw21I GWGCWC 1 cut(s) 445
AlwI GGATC 1 cut(s) 271
AlwNI CAGNNNCTG 2 cut(s) 95, 104
Ama87I CYCGRG 1 cut(s) 515
AoxI GGCC 2 cut(s) 331, 591
ApeKI GCWGC 3 cut(s) 92, 95, 703
ApoI RAATTY 2 cut(s) 239, 362
AsuC2I CCSGG 1 cut(s) 549
AvaI CYCGRG 1 cut(s) 515
BbsI GAAGAC 2 cut(s) 233, 663
Bbv12I GWGCWC 1 cut(s) 445
BbvCI CCTCAGC 1 cut(s) 533
BbvI GCAGC 3 cut(s) 82, 104, 690
BccI CCATC 1 cut(s) 592
BceAI ACGGC 1 cut(s) 423
BciT130I CCWGG 1 cut(s) 12
BciVI GTATCC 1 cut(s) 210
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 549
BfaI CTAG 2 cut(s) 218, 712
BfmI CTRYAG 2 cut(s) 96, 704
BfrI CTTAAG 1 cut(s) 620
BfuI GTATCC 1 cut(s) 210
BisI GCNGC 3 cut(s) 93, 96, 704
BlsI GCNGC 3 cut(s) 94, 97, 705
BmcAI AGTACT 1 cut(s) 683
Bme1390I CCNGG 2 cut(s) 12, 549
BmeT110I CYCGRG 1 cut(s) 515
BmiI GGNNCC 1 cut(s) 406
BmrFI CCNGG 2 cut(s) 12, 549
BpiI GAAGAC 2 cut(s) 233, 663
BpmI CTGGAG 1 cut(s) 294
Bpu10I CCTNAGC 1 cut(s) 533
BpuMI CCSGG 1 cut(s) 549
BsaJI CCNNGG 4 cut(s) 19, 210, 516, 588
BsaWI WCCGGW 1 cut(s) 476
Bsc4I CCNNNNNNNGG 2 cut(s) 194, 676
BseBI CCWGG 1 cut(s) 12
BseDI CCNNGG 4 cut(s) 19, 210, 516, 588
BseGI GGATG 3 cut(s) 315, 436, 584
BseLI CCNNNNNNNGG 2 cut(s) 194, 676
BseMII CTCAG 3 cut(s) 213, 547, 558
BseXI GCAGC 3 cut(s) 82, 104, 690
BseYI CCCAGC 1 cut(s) 320
Bsh1285I CGRYCG 1 cut(s) 481
BshFI GGCC 2 cut(s) 333, 593
BsiEI CGRYCG 1 cut(s) 481
BsiHKAI GWGCWC 1 cut(s) 445
BsiHKCI CYCGRG 1 cut(s) 515
BsiSI CCGG 2 cut(s) 477, 549
BslFI GGGAC 1 cut(s) 545
BslI CCNNNNNNNGG 2 cut(s) 194, 676
BsmFI GGGAC 1 cut(s) 545
BsnI GGCC 2 cut(s) 333, 593
BsoBI CYCGRG 1 cut(s) 515
Bsp1286I GDGCHC 1 cut(s) 445
Bsp143I GATC 3 cut(s) 3, 262, 276
BspACI CCGC 1 cut(s) 137
BspANI GGCC 2 cut(s) 333, 593
BspCNI CTCAG 3 cut(s) 214, 546, 559
BspLI GGNNCC 1 cut(s) 406
BspMAI CTGCAG 2 cut(s) 100, 708
BspPI GGATC 1 cut(s) 271
BspTI CTTAAG 1 cut(s) 620
BssECI CCNNGG 4 cut(s) 19, 210, 516, 588
BssMI GATC 3 cut(s) 3, 262, 276
BssT1I CCWWGG 3 cut(s) 19, 210, 588
Bst2UI CCWGG 1 cut(s) 12
Bst4CI ACNGT 3 cut(s) 64, 106, 274
Bst6I CTCTTC 1 cut(s) 613
BstAFI CTTAAG 1 cut(s) 620
BstC8I GCNNGC 1 cut(s) 389
BstDEI CTNAG 4 cut(s) 116, 222, 533, 567
BstF5I GGATG 3 cut(s) 315, 436, 584
BstKTI GATC 3 cut(s) 6, 265, 279
BstMBI GATC 3 cut(s) 3, 262, 276
BstMCI CGRYCG 1 cut(s) 481
BstMWI GCNNNNNNNGC 2 cut(s) 330, 384
BstNI CCWGG 1 cut(s) 12
BstNSI RCATGY 2 cut(s) 391, 425
BstSCI CCNGG 2 cut(s) 10, 547
BstSFI CTRYAG 2 cut(s) 96, 704
BstV1I GCAGC 3 cut(s) 82, 104, 690
BstV2I GAAGAC 2 cut(s) 233, 663
BstXI CCANNNNNNTGG 1 cut(s) 192
BsuI GTATCC 1 cut(s) 210
BsuRI GGCC 2 cut(s) 333, 593
BtsCI GGATG 3 cut(s) 315, 436, 584
BtsI GCAGTG 2 cut(s) 174, 514
BtsIMutI CAGTG 3 cut(s) 174, 270, 514
Cac8I GCNNGC 1 cut(s) 389
CaiI CAGNNNCTG 2 cut(s) 95, 104
Csp6I GTAC 4 cut(s) 26, 215, 484, 682
CviAII CATG 4 cut(s) 45, 289, 388, 422
CviQI GTAC 4 cut(s) 26, 215, 484, 682
DdeI CTNAG 4 cut(s) 116, 222, 533, 567
DpnI GATC 3 cut(s) 5, 264, 278
DpnII GATC 3 cut(s) 3, 262, 276
Eam1104I CTCTTC 1 cut(s) 613
EarI CTCTTC 1 cut(s) 613
Eco130I CCWWGG 3 cut(s) 19, 210, 588
Eco147I AGGCCT 1 cut(s) 333
Eco32I GATATC 1 cut(s) 545
Eco57I CTGAAG 1 cut(s) 267
Eco88I CYCGRG 1 cut(s) 515
EcoRI GAATTC 1 cut(s) 362
EcoRII CCWGG 1 cut(s) 10
EcoRV GATATC 1 cut(s) 545
EcoT14I CCWWGG 3 cut(s) 19, 210, 588
ErhI CCWWGG 3 cut(s) 19, 210, 588
FaeI CATG 4 cut(s) 48, 292, 391, 425
FaqI GGGAC 1 cut(s) 545
FatI CATG 4 cut(s) 44, 288, 387, 421
FauNDI CATATG 1 cut(s) 488
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 3 cut(s) 93, 96, 704
FokI GGATG 3 cut(s) 302, 443, 571
Fsp4HI GCNGC 3 cut(s) 93, 96, 704
FspBI CTAG 2 cut(s) 218, 712
GluI GCNGC 3 cut(s) 93, 96, 704
GsaI CCCAGC 1 cut(s) 324
GsuI CTGGAG 1 cut(s) 294
HaeIII GGCC 2 cut(s) 333, 593
HapII CCGG 2 cut(s) 477, 549
Hin1II CATG 4 cut(s) 48, 292, 391, 425
HindIII AAGCTT 1 cut(s) 617
HinfI GANTC 2 cut(s) 15, 467
HpaII CCGG 2 cut(s) 477, 549
Hpy166II GTNNAC 1 cut(s) 636
Hpy188I TCNGA 3 cut(s) 39, 157, 613
Hpy188III TCNNGA 4 cut(s) 169, 311, 515, 562
Hpy8I GTNNAC 1 cut(s) 636
HpyAV CCTTC 2 cut(s) 291, 619
HpyCH4III ACNGT 3 cut(s) 64, 106, 274
HpyCH4V TGCA 4 cut(s) 98, 391, 524, 706
HpyF10VI GCNNNNNNNGC 2 cut(s) 330, 384
HpyF3I CTNAG 4 cut(s) 116, 222, 533, 567
Hsp92II CATG 4 cut(s) 48, 292, 391, 425
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 3 cut(s) 3, 262, 276
Lsp1109I GCAGC 3 cut(s) 82, 104, 690
MaeI CTAG 2 cut(s) 218, 712
MaeIII GTNAC 1 cut(s) 100
MalI GATC 3 cut(s) 5, 264, 278
MboI GATC 3 cut(s) 3, 262, 276
MboII GAAGA 4 cut(s) 238, 260, 600, 668
MhlI GDGCHC 1 cut(s) 445
MluCI AATT 3 cut(s) 239, 348, 362
MlyI GAGTC 1 cut(s) 24
MnlI CCTC 5 cut(s) 344, 421, 511, 542, 622
MseI TTAA 5 cut(s) 66, 80, 347, 621, 693
MslI CAYNNNNRTG 2 cut(s) 272, 392
MspA1I CMGCKG 1 cut(s) 95
MspCI CTTAAG 1 cut(s) 620
MspI CCGG 2 cut(s) 477, 549
MspR9I CCNGG 2 cut(s) 12, 549
MvaI CCWGG 1 cut(s) 12
MwoI GCNNNNNNNGC 2 cut(s) 330, 384
NciI CCSGG 1 cut(s) 549
NdeI CATATG 1 cut(s) 488
NdeII GATC 3 cut(s) 3, 262, 276
NlaIII CATG 4 cut(s) 48, 292, 391, 425
NlaIV GGNNCC 1 cut(s) 406
NspI RCATGY 2 cut(s) 391, 425
PaeI GCATGC 1 cut(s) 391
PceI AGGCCT 1 cut(s) 333
PciI ACATGT 1 cut(s) 421
PfeI GAWTC 1 cut(s) 467
PflMI CCANNNNNTGG 1 cut(s) 676
PkrI GCNGC 3 cut(s) 94, 97, 705
PleI GAGTC 1 cut(s) 23
PpsI GAGTC 1 cut(s) 23
PscI ACATGT 1 cut(s) 421
PsiI TTATAA 1 cut(s) 371
Psp6I CCWGG 1 cut(s) 10
PspFI CCCAGC 1 cut(s) 320
PspGI CCWGG 1 cut(s) 10
PspN4I GGNNCC 1 cut(s) 406
PsrI GAACNNNNNNTAC 2 cut(s) 18, 50
PstI CTGCAG 2 cut(s) 100, 708
PstNI CAGNNNCTG 2 cut(s) 95, 104
PvuII CAGCTG 1 cut(s) 95
RsaI GTAC 4 cut(s) 27, 216, 485, 683
RsaNI GTAC 4 cut(s) 26, 215, 484, 682
RseI CAYNNNNRTG 2 cut(s) 272, 392
SaqAI TTAA 5 cut(s) 66, 80, 347, 621, 693
SatI GCNGC 3 cut(s) 93, 96, 704
Sau3AI GATC 3 cut(s) 3, 262, 276
ScaI AGTACT 1 cut(s) 683
SchI GAGTC 1 cut(s) 24
ScrFI CCNGG 2 cut(s) 12, 549
SduI GDGCHC 1 cut(s) 445
SfcI CTRYAG 2 cut(s) 96, 704
SmiMI CAYNNNNRTG 2 cut(s) 272, 392
SmlI CTYRAG 1 cut(s) 620
SmoI CTYRAG 1 cut(s) 620
SphI GCATGC 1 cut(s) 391
Sse9I AATT 3 cut(s) 239, 348, 362
SseBI AGGCCT 1 cut(s) 333
SsiI CCGC 1 cut(s) 137
SspMI CTAG 2 cut(s) 218, 712
StuI AGGCCT 1 cut(s) 333
StyD4I CCNGG 2 cut(s) 10, 547
StyI CCWWGG 3 cut(s) 19, 210, 588
TaaI ACNGT 3 cut(s) 64, 106, 274
TasI AATT 3 cut(s) 239, 348, 362
TatI WGTACW 1 cut(s) 681
TfiI GAWTC 1 cut(s) 467
Tru1I TTAA 5 cut(s) 66, 80, 347, 621, 693
Tru9I TTAA 5 cut(s) 66, 80, 347, 621, 693
TscAI CASTG 3 cut(s) 181, 277, 514
TseI GCWGC 3 cut(s) 92, 95, 703
TspDTI ATGAA 1 cut(s) 375
TspRI CASTG 3 cut(s) 181, 277, 514
Van91I CCANNNNNTGG 1 cut(s) 676
Vha464I CTTAAG 1 cut(s) 620
XapI RAATTY 2 cut(s) 239, 362
XceI RCATGY 2 cut(s) 391, 425
XcmI CCANNNNNNNNNTGG 1 cut(s) 421
XspI CTAG 2 cut(s) 218, 712
ZrmI AGTACT 1 cut(s) 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.