AT1G61900

gpi-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007201
Physical Location & Seq
Reverse (-)
22882176 .. 22885125
2950 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G61900.1

Sequence Viewer

Length: 1302 bp
ATGACGAGAAGAGCTGAATTTGAGATGGGTTTGTTCGTGATTCTTCAATCCATGTTTCTGATATCTCTATGTTCATCACAGAAACCTGAGGAGTTCTTACCTGAGATATCACCAGATACTTCTCCTCAACCGTTTCTTCCTTTCATTGCACCTTCTCCAATGGTTCCATACATAAATAGTACCATGCCTAAACTATCAGGGCTTTGCTCGCTTAATTTCAGTGCGTCTGAGAGTTTGATTCAGACTACTTCACACAACTGTTGGACTGTTTTTGCTCCATTGTTGGCAAATGTGATGTGTTGTCCTCAGCTAGATGCTACACTCACAATAATCCTTGGTAAAGCGAGTAAAGAAACCGGTTTGCTTGCTTTAAACAGAACCCAGTCTAAGCACTGTCTCTCGGATTTAGAGCAAATCTTGGTAGGAAAAGGCGCTTCAGGTCAGCTTAATAAGATCTGTTCTATTCACTCGTCGAATCTCACTTCTTCGTCGTGCCCTGTTATCAATGTCGATGAGTTTGAAAGCACGGTGGATACAGCGAAACTTCTTTTGGCCTGTGAAAAAATTGATCCTGTGAAGGAATGTTGCGAAGAGGCTTGCCAAAATGCGATACTCGATGCTGCTACGAATATATCTCTTAAAGCATCAGAAACTTTAACTGATAATTCGGATAGGATCAATGACTGCAAGAACGTAGTAAACCGCTGGCTCGCTACTAAACTTGACCCTTCTCGGGTTAAGGAAACTCTCAGAGGATTAGCAAACTGCAAAATCAATAGAGTTTGTCCTCTTGTCTTTCCGCACATGAAGCATATTGGTGGTAACTGCAGCAACGAGTTGAGTAACCAAACGGGTTGTTGCCGTGCAATGGAGAGTTATGTGTCTCACTTGCAAAAGCAAACACTTATAACCAATTTGCAAGCTTTAGATTGCGCAACCAGCCTTGGGACAAAGTTACAGAAACTGAACATCACTAAGAACATATTCAGTGTTTGTCATATAAGTCTCAAGGACTTCTCTCTTCAAGTTGGAAACCAAGAATCTGGTTGTCTATTACCGAGCCTACCATCGGATGCGATATTTGATAAAGACACGGGGATAAGCTTTACTTGTGACCTCAATGACAACATTCCCGCTCCGTGGCCTTCATCTTCTCTATCCTCAGCCTCTACCTGTAAAAAACCTGTTAGAATTCCTGCTCTTCCAGCTGCTGCCTCCTCGCAACCTCGTCTCCACGATGAAGGAGTGACACGTTTGGTGATCTTTGTCTTGTCTATGCTTCTTGTGATGCTTCTGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

433

Amino Acids

47.03

Weight (kDa)

6.26

Isoelectric Point (pI)

53.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPARK PF19160 66 - 229 2.1e-22 SPARK
At1g61900 PF26584 260 - 333 2.2e-33 At1g61900-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 908
Acc16I TGCGCA 1 cut(s) 934
AccBSI CCGCTC 1 cut(s) 1136
AciI CCGC 3 cut(s) 703, 800, 1134
AclWI GGATC 2 cut(s) 563, 683
AcsI RAATTY 2 cut(s) 17, 1191
AcuI CTGAAG 1 cut(s) 420
AfaI GTAC 1 cut(s) 181
AfiI CCNNNNNNNGG 5 cut(s) 733, 868, 945, 1069, 1140
AflIII ACRYGT 1 cut(s) 1250
AgeI ACCGGT 1 cut(s) 356
AgsI TTSAA 3 cut(s) 47, 521, 1025
AjuI GAANNNNNNNTTGG 2 cut(s) 533, 565
AluBI AGCT 6 cut(s) 14, 310, 445, 923, 1104, 1208
AluI AGCT 6 cut(s) 14, 310, 445, 923, 1104, 1208
Alw26I GTCTC 4 cut(s) 401, 888, 1010, 1235
AlwI GGATC 2 cut(s) 563, 683
AlwNI CAGNNNCTG 2 cut(s) 964, 1211
Ama87I CYCGRG 1 cut(s) 732
AoxI GGCC 2 cut(s) 552, 1142
ApeKI GCWGC 4 cut(s) 620, 828, 1208, 1211
ApoI RAATTY 2 cut(s) 17, 1191
AsiGI ACCGGT 1 cut(s) 356
Asp700I GAANNNNTTC 1 cut(s) 983
AspLEI GCGC 2 cut(s) 434, 935
AsuHPI GGTGA 2 cut(s) 102, 1270
AvaI CYCGRG 1 cut(s) 732
AxyI CCTNAGG 1 cut(s) 87
BaeGI GKGCMC 1 cut(s) 497
BbvCI CCTCAGC 2 cut(s) 306, 1162
BbvI GCAGC 4 cut(s) 607, 840, 1195, 1198
BccI CCATC 2 cut(s) 19, 1075
BceAI ACGGC 1 cut(s) 846
BciVI GTATCC 1 cut(s) 526
BcoDI GTCTC 4 cut(s) 401, 888, 1010, 1235
BfaI CTAG 1 cut(s) 311
BfmI CTRYAG 1 cut(s) 826
BfoI RGCGCY 1 cut(s) 435
BfuI GTATCC 1 cut(s) 526
BglII AGATCT 1 cut(s) 453
BisI GCNGC 4 cut(s) 621, 829, 1209, 1212
BlsI GCNGC 4 cut(s) 622, 830, 1210, 1213
BmeT110I CYCGRG 1 cut(s) 732
BmiI GGNNCC 1 cut(s) 165
BmrI ACTGGG 1 cut(s) 376
BmsI GCATC 5 cut(s) 304, 607, 653, 1063, 1278
BmuI ACTGGG 1 cut(s) 376
Bpu10I CCTNAGC 2 cut(s) 306, 1162
BpuEI CTTGAG 1 cut(s) 992
BsaJI CCNNGG 3 cut(s) 334, 943, 1139
BsaWI WCCGGW 1 cut(s) 356
BsaXI ACNNNNNCTCC 2 cut(s) 1215, 1245
Bsc4I CCNNNNNNNGG 5 cut(s) 733, 868, 945, 1069, 1140
Bse118I RCCGGY 1 cut(s) 356
Bse1I ACTGG 1 cut(s) 382
Bse21I CCTNAGG 1 cut(s) 87
Bse3DI GCAATG 2 cut(s) 144, 873
BseDI CCNNGG 3 cut(s) 334, 943, 1139
BseGI GGATG 1 cut(s) 1078
BseLI CCNNNNNNNGG 5 cut(s) 733, 868, 945, 1069, 1140
BseMI GCAATG 2 cut(s) 144, 873
BseMII CTCAG 6 cut(s) 78, 93, 219, 320, 763, 1176
BseNI ACTGG 1 cut(s) 382
BseRI GAGGAG 3 cut(s) 104, 114, 1207
BseSI GKGCMC 1 cut(s) 497
BseXI GCAGC 4 cut(s) 607, 840, 1195, 1198
BshFI GGCC 2 cut(s) 554, 1144
BshTI ACCGGT 1 cut(s) 356
BsiHKCI CYCGRG 1 cut(s) 732
BsiSI CCGG 1 cut(s) 357
BslFI GGGAC 1 cut(s) 961
BslI CCNNNNNNNGG 5 cut(s) 733, 868, 945, 1069, 1140
BsmAI GTCTC 4 cut(s) 401, 888, 1010, 1235
BsmBI CGTCTC 1 cut(s) 1235
BsmFI GGGAC 1 cut(s) 961
BsnI GGCC 2 cut(s) 554, 1144
BsoBI CYCGRG 1 cut(s) 732
Bsp1286I GDGCHC 1 cut(s) 497
Bsp143I GATC 4 cut(s) 453, 568, 675, 1260
BspACI CCGC 3 cut(s) 703, 800, 1134
BspANI GGCC 2 cut(s) 554, 1144
BspCNI CTCAG 6 cut(s) 79, 94, 220, 319, 762, 1175
BspLI GGNNCC 1 cut(s) 165
BspMAI CTGCAG 1 cut(s) 830
BspPI GGATC 2 cut(s) 563, 683
BspQI GCTCTTC 2 cut(s) 4, 1206
BsrBI CCGCTC 1 cut(s) 1136
BsrDI GCAATG 2 cut(s) 144, 873
BsrFI RCCGGY 1 cut(s) 356
BsrI ACTGG 1 cut(s) 382
BssAI RCCGGY 1 cut(s) 356
BssECI CCNNGG 3 cut(s) 334, 943, 1139
BssMI GATC 4 cut(s) 453, 568, 675, 1260
BssT1I CCWWGG 2 cut(s) 334, 943
Bst4CI ACNGT 5 cut(s) 132, 260, 268, 395, 529
Bst6I CTCTTC 4 cut(s) 4, 585, 1026, 1206
BstC8I GCNNGC 6 cut(s) 209, 366, 598, 707, 711, 921
BstDEI CTNAG 8 cut(s) 87, 102, 228, 306, 387, 749, 975, 1162
BstDSI CCRYGG 1 cut(s) 1139
BstF5I GGATG 1 cut(s) 1078
BstH2I RGCGCY 1 cut(s) 435
BstHHI GCGC 2 cut(s) 434, 935
BstKTI GATC 4 cut(s) 456, 571, 678, 1263
BstMAI GTCTC 4 cut(s) 401, 888, 1010, 1235
BstMBI GATC 4 cut(s) 453, 568, 675, 1260
BstMWI GCNNNNNNNGC 4 cut(s) 208, 808, 939, 1205
BstSFI CTRYAG 1 cut(s) 826
BstSLI GKGCMC 1 cut(s) 497
BstV1I GCAGC 4 cut(s) 607, 840, 1195, 1198
BstX2I RGATCY 1 cut(s) 453
BstXI CCANNNNNNTGG 1 cut(s) 1043
BstYI RGATCY 1 cut(s) 453
Bsu36I CCTNAGG 1 cut(s) 87
BsuI GTATCC 1 cut(s) 526
BsuRI GGCC 2 cut(s) 554, 1144
BtgI CCRYGG 1 cut(s) 1139
BtsCI GGATG 1 cut(s) 1078
BtsIMutI CAGTG 3 cut(s) 226, 391, 994
Cac8I GCNNGC 6 cut(s) 209, 366, 598, 707, 711, 921
CaiI CAGNNNCTG 2 cut(s) 964, 1211
CfoI GCGC 2 cut(s) 434, 935
Cfr10I RCCGGY 1 cut(s) 356
CseI GACGC 1 cut(s) 213
Csp6I GTAC 1 cut(s) 180
CspAI ACCGGT 1 cut(s) 356
CviAII CATG 3 cut(s) 52, 184, 805
CviQI GTAC 1 cut(s) 180
DdeI CTNAG 8 cut(s) 87, 102, 228, 306, 387, 749, 975, 1162
DpnI GATC 4 cut(s) 455, 570, 677, 1262
DpnII GATC 4 cut(s) 453, 568, 675, 1260
DraI TTTAAA 1 cut(s) 372
Eam1104I CTCTTC 4 cut(s) 4, 585, 1026, 1206
EarI CTCTTC 4 cut(s) 4, 585, 1026, 1206
Eco130I CCWWGG 2 cut(s) 334, 943
Eco32I GATATC 2 cut(s) 63, 108
Eco57I CTGAAG 1 cut(s) 420
Eco81I CCTNAGG 1 cut(s) 87
Eco88I CYCGRG 1 cut(s) 732
EcoRI GAATTC 1 cut(s) 1191
EcoRV GATATC 2 cut(s) 63, 108
EcoT14I CCWWGG 2 cut(s) 334, 943
ErhI CCWWGG 2 cut(s) 334, 943
Esp3I CGTCTC 1 cut(s) 1235
FaeI CATG 3 cut(s) 55, 187, 808
FalI AAGNNNNNCTT 2 cut(s) 1093, 1125
FaqI GGGAC 1 cut(s) 961
FatI CATG 3 cut(s) 51, 183, 804
FauI CCCGC 1 cut(s) 1141
Fnu4HI GCNGC 4 cut(s) 621, 829, 1209, 1212
FokI GGATG 1 cut(s) 1085
Fsp4HI GCNGC 4 cut(s) 621, 829, 1209, 1212
FspBI CTAG 1 cut(s) 311
FspI TGCGCA 1 cut(s) 934
GlaI GCGC 2 cut(s) 433, 934
GluI GCNGC 4 cut(s) 621, 829, 1209, 1212
HaeII RGCGCY 1 cut(s) 435
HaeIII GGCC 2 cut(s) 554, 1144
HapII CCGG 1 cut(s) 357
HgaI GACGC 1 cut(s) 213
HhaI GCGC 2 cut(s) 434, 935
Hin1II CATG 3 cut(s) 55, 187, 808
Hin6I GCGC 2 cut(s) 432, 933
HinP1I GCGC 2 cut(s) 432, 933
HindIII AAGCTT 2 cut(s) 921, 1102
HinfI GANTC 4 cut(s) 40, 238, 475, 1040
HpaII CCGG 1 cut(s) 357
HphI GGTGA 2 cut(s) 102, 1270
Hpy166II GTNNAC 1 cut(s) 700
Hpy188I TCNGA 8 cut(s) 60, 229, 243, 403, 649, 670, 752, 1072
Hpy188III TCNNGA 1 cut(s) 37
Hpy8I GTNNAC 1 cut(s) 700
Hpy99I CGWCG 2 cut(s) 475, 493
HpyAV CCTTC 5 cut(s) 162, 571, 738, 1155, 1235
HpyCH4III ACNGT 5 cut(s) 132, 260, 268, 395, 529
HpyCH4IV ACGT 2 cut(s) 693, 1252
HpyCH4V TGCA 7 cut(s) 149, 687, 768, 828, 866, 892, 919
HpyF10VI GCNNNNNNNGC 4 cut(s) 208, 808, 939, 1205
HpyF3I CTNAG 8 cut(s) 87, 102, 228, 306, 387, 749, 975, 1162
HpySE526I ACGT 2 cut(s) 693, 1252
Hsp92II CATG 3 cut(s) 55, 187, 808
HspAI GCGC 2 cut(s) 432, 933
Kzo9I GATC 4 cut(s) 453, 568, 675, 1260
LguI GCTCTTC 2 cut(s) 4, 1206
LmnI GCTCC 2 cut(s) 280, 1141
Lsp1109I GCAGC 4 cut(s) 607, 840, 1195, 1198
LweI GCATC 5 cut(s) 304, 607, 653, 1063, 1278
MaeI CTAG 1 cut(s) 311
MaeII ACGT 2 cut(s) 693, 1252
MaeIII GTNAC 5 cut(s) 821, 842, 954, 1112, 1246
MalI GATC 4 cut(s) 455, 570, 677, 1262
MbiI CCGCTC 1 cut(s) 1136
MboI GATC 4 cut(s) 453, 568, 675, 1260
MboII GAAGA 8 cut(s) 21, 35, 128, 477, 602, 1013, 1143, 1193
MflI RGATCY 1 cut(s) 453
MhlI GDGCHC 1 cut(s) 497
MluCI AATT 6 cut(s) 17, 214, 564, 664, 913, 1191
MmeI TCCRAC 2 cut(s) 242, 1009
MroXI GAANNNNTTC 1 cut(s) 983
MseI TTAA 6 cut(s) 213, 371, 447, 639, 656, 738
MslI CAYNNNNRTG 1 cut(s) 816
MspA1I CMGCKG 2 cut(s) 705, 1208
MspI CCGG 1 cut(s) 357
MwoI GCNNNNNNNGC 4 cut(s) 208, 808, 939, 1205
NdeII GATC 4 cut(s) 453, 568, 675, 1260
NlaIII CATG 3 cut(s) 55, 187, 808
NlaIV GGNNCC 1 cut(s) 165
NmuCI GTSAC 2 cut(s) 1112, 1246
NsbI TGCGCA 1 cut(s) 934
PciSI GCTCTTC 2 cut(s) 4, 1206
PdmI GAANNNNTTC 1 cut(s) 983
PfeI GAWTC 4 cut(s) 40, 238, 475, 1040
PinAI ACCGGT 1 cut(s) 356
PkrI GCNGC 4 cut(s) 622, 830, 1210, 1213
PsiI TTATAA 1 cut(s) 908
PspN4I GGNNCC 1 cut(s) 165
PstI CTGCAG 1 cut(s) 830
PstNI CAGNNNCTG 2 cut(s) 964, 1211
PsuI RGATCY 1 cut(s) 453
PvuII CAGCTG 1 cut(s) 1208
RsaI GTAC 1 cut(s) 181
RsaNI GTAC 1 cut(s) 180
RseI CAYNNNNRTG 1 cut(s) 816
SapI GCTCTTC 2 cut(s) 4, 1206
SaqAI TTAA 6 cut(s) 213, 371, 447, 639, 656, 738
SatI GCNGC 4 cut(s) 621, 829, 1209, 1212
Sau3AI GATC 4 cut(s) 453, 568, 675, 1260
SduI GDGCHC 1 cut(s) 497
SfaNI GCATC 5 cut(s) 304, 607, 653, 1063, 1278
SfcI CTRYAG 1 cut(s) 826
SmiMI CAYNNNNRTG 1 cut(s) 816
SmlI CTYRAG 1 cut(s) 1007
SmoI CTYRAG 1 cut(s) 1007
Sse9I AATT 6 cut(s) 17, 214, 564, 664, 913, 1191
SsiI CCGC 3 cut(s) 703, 800, 1134
SspMI CTAG 1 cut(s) 311
StyI CCWWGG 2 cut(s) 334, 943
TaaI ACNGT 5 cut(s) 132, 260, 268, 395, 529
TaiI ACGT 2 cut(s) 696, 1255
TaqI TCGA 3 cut(s) 473, 510, 615
TasI AATT 6 cut(s) 17, 214, 564, 664, 913, 1191
TfiI GAWTC 4 cut(s) 40, 238, 475, 1040
Tru1I TTAA 6 cut(s) 213, 371, 447, 639, 656, 738
Tru9I TTAA 6 cut(s) 213, 371, 447, 639, 656, 738
TscAI CASTG 3 cut(s) 226, 398, 994
TseFI GTSAC 2 cut(s) 1112, 1246
TseI GCWGC 4 cut(s) 620, 828, 1208, 1211
Tsp45I GTSAC 2 cut(s) 1112, 1246
TspDTI ATGAA 5 cut(s) 63, 133, 821, 1137, 1254
TspGWI ACGGA 1 cut(s) 1128
TspRI CASTG 3 cut(s) 226, 398, 994
XapI RAATTY 2 cut(s) 17, 1191
XmnI GAANNNNTTC 1 cut(s) 983
XspI CTAG 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.