Prupe.4G021700_v2.0.a1

GPI-anchored protein At1g61900-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0007201
Physical Location & Seq
Forward (+)
1027551 .. 1031803
4253 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G021700.1

Sequence Viewer

Length: 1395 bp
ATGGTTTCTCTATTTTGGGGTAAGGAAATTGAAGGCATGAAGGAAACGGTGTCTCTGAAGCTCACCGTCCATATATTTATGTTGTTCCTATGTCTCCATATATGTTGTGGCAGCACGTTAACTTATCATAAAGATCCAGACTTGACGATTAAACAAGAAGATTCTATGTTACCGATGATCTCTCCTACAGGGACTCCTCAGCCCTTTCTTCCCCTTCTGGCTCCTTCACCACTGGCACCGTTCACAAATACCACTGTCCCAAAATTATCAGGACTCTGTACGCTAAACTTCAGTGTTGCTGAGAGTTTGATGAGTGTGACCTCAGTTGATTGTTGGTCGGTTTTTGCACCACTGCTCGCTAATGTAATTTGTTGCCCACAGTTGGAAGCCACTCTCACAATTCTCATTGGTCAATCCAGTAAAGAGACCAATGTTCTTGCTTTAAATGGGACGGTTGCTAAACATTGTCTATCAGATATTGAGCAGATTTTGGTGGGCCAGGGTGCAAATGACAGTCTTGCACAAATATGCTCGGTTCATTCATCAAATCTGACTTATGCATCTTGCCCAGTCATTGATGTTAATGAGTTTGAGGATACAGTTGATACTTCCAAGTTGCTTGCCGCTTGTGAGAAGATTGACCCTGTGAAAGAATGCTGTGCTCAAACTTGTCAAAATGCCATATTAGAAGCTGCTACGAGGATCGCATCAAAAGCTTCTGATCTTTTGAGCACGGATGGTGCTCACGATATCTTACCTGATGGTGTAAGTAAAGGCAATGACTGCAAAAATATTGTCCTCCGGTGGCTGGCAAGTAAACTCAATCCTTCTCGTGCAAAGGAAGTTCTAAGGGGACTATCTAATTGCAAGATTAACAAGGTTTGTCCCCTGGTTTTCCCCAACATGAAGCATGTTGCAATGGGTTGTGGGAATGGGATAAGAAACCAGGCTGCTTGTTGTACTGCCATGGAGAGCTATGTGTCCCACTTGCAAAAGCAGAGTTTCATAACCAACTTGCAAGCTTTGGATTGTGCTGCATCACTGGGCATGAAGTTAAGAAAATCAAATATTACTGAAAATGTGTACAGCCTATGTCATATAAGCCTCAAGGATTTCTCCCTCCAAGTTGGAAATCAAGTGTCTGGATGTCTTCTGCCGAGCTTGCCTTCCGATGCAACATTTGACAGTTCAGGGGTCAGCTTCCTTTGTGATCTGAATGATAACATTCCAGCTCCCTGGCCCACCTCTTCTCAAGGGGCAGGTTCATCATGCAGTAAAAATGTCAAGATTCCCGCACTTCCTGCAGCAGCATCTGCTGAAAGTGGTCTGTACAATGATGATGTGATGCTTTCTCTACTCCTTGCTTCTGGGGTGGTCCTCATGATGGTTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

465

Amino Acids

49.45

Weight (kDa)

5.49

Isoelectric Point (pI)

49.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1250
AccB1I GGYRCC 1 cut(s) 235
AciI CCGC 2 cut(s) 624, 1293
AclWI GGATC 2 cut(s) 128, 710
AcuI CTGAAG 2 cut(s) 77, 274
AfaI GTAC 4 cut(s) 280, 961, 1085, 1331
AfiI CCNNNNNNNGG 3 cut(s) 382, 808, 1384
AgsI TTSAA 1 cut(s) 32
AjnI CCWGG 4 cut(s) 498, 888, 945, 1235
AluBI AGCT 8 cut(s) 61, 692, 716, 975, 1022, 1161, 1200, 1232
AluI AGCT 8 cut(s) 61, 692, 716, 975, 1022, 1161, 1200, 1232
Alw21I GWGCWC 3 cut(s) 664, 734, 745
Alw26I GTCTC 3 cut(s) 57, 98, 419
AlwI GGATC 2 cut(s) 128, 710
AlwNI CAGNNNCTG 1 cut(s) 1313
AoxI GGCC 2 cut(s) 496, 1238
ApeKI GCWGC 6 cut(s) 111, 692, 950, 1034, 1304, 1307
ArsI GACNNNNNNTTYG 2 cut(s) 780, 812
AspS9I GGNCC 3 cut(s) 496, 1239, 1375
AsuHPI GGTGA 2 cut(s) 55, 219
AvaII GGWCC 1 cut(s) 1375
BanI GGYRCC 1 cut(s) 235
BauI CACGAG 1 cut(s) 831
BbsI GAAGAC 1 cut(s) 1142
Bbv12I GWGCWC 3 cut(s) 664, 734, 745
BbvCI CCTCAGC 1 cut(s) 198
BbvI GCAGC 6 cut(s) 123, 679, 937, 1021, 1316, 1319
BccI CCATC 3 cut(s) 731, 755, 1378
BciT130I CCWGG 4 cut(s) 500, 890, 947, 1237
BciVI GTATCC 1 cut(s) 589
BcoDI GTCTC 3 cut(s) 57, 98, 419
BfmI CTRYAG 2 cut(s) 186, 1302
BfuAI ACCTGC 1 cut(s) 1250
BfuI GTATCC 1 cut(s) 589
BisI GCNGC 7 cut(s) 112, 624, 693, 951, 1035, 1305, 1308
BlsI GCNGC 7 cut(s) 113, 625, 694, 952, 1036, 1306, 1309
Bme1390I CCNGG 4 cut(s) 500, 890, 947, 1237
Bme18I GGWCC 1 cut(s) 1375
BmgT120I GGNCC 3 cut(s) 496, 1239, 1375
BmiI GGNNCC 2 cut(s) 222, 237
BmrFI CCNGG 4 cut(s) 500, 890, 947, 1237
BmrI ACTGGG 2 cut(s) 563, 1052
BmsI GCATC 6 cut(s) 569, 716, 1046, 1162, 1319, 1335
BmuI ACTGGG 2 cut(s) 563, 1052
BpiI GAAGAC 1 cut(s) 1142
Bpu10I CCTNAGC 1 cut(s) 198
BpuEI CTTGAG 2 cut(s) 1091, 1236
BsaI GGTCTC 1 cut(s) 419
BsaJI CCNNGG 4 cut(s) 499, 888, 966, 1235
BsaWI WCCGGW 1 cut(s) 801
BsaXI ACNNNNNCTCC 2 cut(s) 178, 208
Bsc4I CCNNNNNNNGG 3 cut(s) 382, 808, 1384
Bse1I ACTGG 4 cut(s) 237, 417, 569, 1047
Bse3DI GCAATG 2 cut(s) 784, 924
BseBI CCWGG 4 cut(s) 500, 890, 947, 1237
BseDI CCNNGG 4 cut(s) 499, 888, 966, 1235
BseGI GGATG 2 cut(s) 742, 1151
BseLI CCNNNNNNNGG 3 cut(s) 382, 808, 1384
BseMI GCAATG 2 cut(s) 784, 924
BseMII CTCAG 3 cut(s) 212, 291, 336
BseNI ACTGG 4 cut(s) 237, 417, 569, 1047
BseRI GAGGAG 1 cut(s) 186
BseXI GCAGC 6 cut(s) 123, 679, 937, 1021, 1316, 1319
BshFI GGCC 2 cut(s) 498, 1240
BshNI GGYRCC 1 cut(s) 235
BsiHKAI GWGCWC 3 cut(s) 664, 734, 745
BsiSI CCGG 1 cut(s) 802
BslFI GGGAC 6 cut(s) 205, 242, 463, 867, 870, 967
BslI CCNNNNNNNGG 3 cut(s) 382, 808, 1384
BsmAI GTCTC 3 cut(s) 57, 98, 419
BsmFI GGGAC 6 cut(s) 205, 242, 463, 867, 870, 967
BsmI GAATGC 1 cut(s) 659
BsnI GGCC 2 cut(s) 498, 1240
Bso31I GGTCTC 1 cut(s) 419
Bsp1286I GDGCHC 3 cut(s) 664, 734, 745
Bsp1407I TGTACA 2 cut(s) 1083, 1329
Bsp143I GATC 5 cut(s) 133, 177, 702, 721, 1210
Bsp19I CCATGG 1 cut(s) 966
BspACI CCGC 2 cut(s) 624, 1293
BspANI GGCC 2 cut(s) 498, 1240
BspCNI CTCAG 3 cut(s) 211, 292, 335
BspHI TCATGA 1 cut(s) 1380
BspLI GGNNCC 2 cut(s) 222, 237
BspMAI CTGCAG 1 cut(s) 1306
BspMI ACCTGC 1 cut(s) 1250
BspPI GGATC 2 cut(s) 128, 710
BspT107I GGYRCC 1 cut(s) 235
BspTNI GGTCTC 1 cut(s) 419
BsrDI GCAATG 2 cut(s) 784, 924
BsrGI TGTACA 2 cut(s) 1083, 1329
BsrI ACTGG 4 cut(s) 237, 417, 569, 1047
BssECI CCNNGG 4 cut(s) 499, 888, 966, 1235
BssMI GATC 5 cut(s) 133, 177, 702, 721, 1210
BssSI CACGAG 1 cut(s) 831
BssT1I CCWWGG 1 cut(s) 966
Bst2BI CACGAG 1 cut(s) 831
Bst2UI CCWGG 4 cut(s) 500, 890, 947, 1237
Bst4CI ACNGT 9 cut(s) 49, 67, 240, 256, 381, 454, 515, 601, 1187
Bst6I CTCTTC 1 cut(s) 1252
BstAPI GCANNNNNTGC 3 cut(s) 783, 1301, 1313
BstAUI TGTACA 2 cut(s) 1083, 1329
BstC8I GCNNGC 5 cut(s) 357, 621, 810, 1020, 1163
BstDEI CTNAG 4 cut(s) 198, 300, 322, 848
BstDSI CCRYGG 1 cut(s) 966
BstF5I GGATG 2 cut(s) 742, 1151
BstKTI GATC 5 cut(s) 136, 180, 705, 724, 1213
BstMAI GTCTC 3 cut(s) 57, 98, 419
BstMBI GATC 5 cut(s) 133, 177, 702, 721, 1210
BstMWI GCNNNNNNNGC 5 cut(s) 713, 783, 1162, 1301, 1313
BstNI CCWGG 4 cut(s) 500, 890, 947, 1237
BstNSI RCATGY 1 cut(s) 914
BstSCI CCNGG 4 cut(s) 498, 888, 945, 1235
BstSFI CTRYAG 2 cut(s) 186, 1302
BstV1I GCAGC 6 cut(s) 123, 679, 937, 1021, 1316, 1319
BstV2I GAAGAC 1 cut(s) 1142
BstX2I RGATCY 1 cut(s) 133
BstXI CCANNNNNNTGG 1 cut(s) 1236
BstYI RGATCY 1 cut(s) 133
BsuI GTATCC 1 cut(s) 589
BsuRI GGCC 2 cut(s) 498, 1240
BtgI CCRYGG 1 cut(s) 966
BtsCI GGATG 2 cut(s) 742, 1151
BtsI GCAGTG 1 cut(s) 350
BtsIMutI CAGTG 5 cut(s) 230, 252, 298, 350, 1040
BveI ACCTGC 1 cut(s) 1250
Cac8I GCNNGC 5 cut(s) 357, 621, 810, 1020, 1163
CaiI CAGNNNCTG 1 cut(s) 1313
CciI TCATGA 1 cut(s) 1380
Cfr13I GGNCC 3 cut(s) 496, 1239, 1375
Csp6I GTAC 4 cut(s) 279, 960, 1084, 1330
CviAII CATG 7 cut(s) 37, 904, 911, 967, 1048, 1269, 1381
CviQI GTAC 4 cut(s) 279, 960, 1084, 1330
DdeI CTNAG 4 cut(s) 198, 300, 322, 848
DpnI GATC 5 cut(s) 135, 179, 704, 723, 1212
DpnII GATC 5 cut(s) 133, 177, 702, 721, 1210
DraI TTTAAA 1 cut(s) 444
Eam1104I CTCTTC 1 cut(s) 1252
EarI CTCTTC 1 cut(s) 1252
Eco130I CCWWGG 1 cut(s) 966
Eco31I GGTCTC 1 cut(s) 419
Eco32I GATATC 1 cut(s) 751
Eco47I GGWCC 1 cut(s) 1375
Eco57I CTGAAG 2 cut(s) 77, 274
EcoRII CCWGG 4 cut(s) 498, 888, 945, 1235
EcoRV GATATC 1 cut(s) 751
EcoT14I CCWWGG 1 cut(s) 966
EcoT22I ATGCAT 1 cut(s) 562
ErhI CCWWGG 1 cut(s) 966
FaeI CATG 7 cut(s) 40, 907, 914, 970, 1051, 1272, 1384
FaqI GGGAC 6 cut(s) 205, 242, 463, 867, 870, 967
FatI CATG 7 cut(s) 36, 903, 910, 966, 1047, 1268, 1380
FauI CCCGC 1 cut(s) 1300
Fnu4HI GCNGC 7 cut(s) 112, 624, 693, 951, 1035, 1305, 1308
FokI GGATG 2 cut(s) 749, 1158
Fsp4HI GCNGC 7 cut(s) 112, 624, 693, 951, 1035, 1305, 1308
GluI GCNGC 7 cut(s) 112, 624, 693, 951, 1035, 1305, 1308
HaeIII GGCC 2 cut(s) 498, 1240
HapII CCGG 1 cut(s) 802
Hin1II CATG 7 cut(s) 40, 907, 914, 970, 1051, 1272, 1384
HincII GTYRAC 1 cut(s) 120
HindII GTYRAC 1 cut(s) 120
HindIII AAGCTT 2 cut(s) 714, 1020
HinfI GANTC 4 cut(s) 161, 193, 273, 1288
HpaI GTTAAC 1 cut(s) 120
HpaII CCGG 1 cut(s) 802
HphI GGTGA 2 cut(s) 55, 219
Hpy166II GTNNAC 4 cut(s) 120, 243, 818, 1084
Hpy188I TCNGA 6 cut(s) 57, 475, 552, 721, 1171, 1215
Hpy188III TCNNGA 6 cut(s) 137, 270, 746, 1143, 1285, 1381
Hpy8I GTNNAC 4 cut(s) 120, 243, 818, 1084
HpyAV CCTTC 6 cut(s) 26, 34, 224, 234, 837, 1176
HpyCH4III ACNGT 9 cut(s) 49, 67, 240, 256, 381, 454, 515, 601, 1187
HpyCH4IV ACGT 1 cut(s) 116
HpyF10VI GCNNNNNNNGC 5 cut(s) 713, 783, 1162, 1301, 1313
HpyF3I CTNAG 4 cut(s) 198, 300, 322, 848
HpySE526I ACGT 1 cut(s) 116
Hsp92II CATG 7 cut(s) 40, 907, 914, 970, 1051, 1272, 1384
KspAI GTTAAC 1 cut(s) 120
Kzo9I GATC 5 cut(s) 133, 177, 702, 721, 1210
LmnI GCTCC 2 cut(s) 226, 1237
Lsp1109I GCAGC 6 cut(s) 123, 679, 937, 1021, 1316, 1319
LweI GCATC 6 cut(s) 569, 716, 1046, 1162, 1319, 1335
MaeII ACGT 1 cut(s) 116
MaeIII GTNAC 2 cut(s) 168, 316
MalI GATC 5 cut(s) 135, 179, 704, 723, 1212
MboI GATC 5 cut(s) 133, 177, 702, 721, 1210
MboII GAAGA 5 cut(s) 170, 200, 646, 1142, 1239
MflI RGATCY 1 cut(s) 133
MhlI GDGCHC 3 cut(s) 664, 734, 745
MluCI AATT 5 cut(s) 27, 263, 366, 399, 862
MlyI GAGTC 2 cut(s) 187, 267
MmeI TCCRAC 2 cut(s) 363, 1108
MnlI CCTC 9 cut(s) 207, 331, 586, 693, 809, 1115, 1130, 1255, 1388
Mph1103I ATGCAT 1 cut(s) 562
MseI TTAA 6 cut(s) 119, 150, 443, 582, 873, 1055
MslI CAYNNNNRTG 1 cut(s) 526
MspI CCGG 1 cut(s) 802
MspR9I CCNGG 4 cut(s) 500, 890, 947, 1237
Mva1269I GAATGC 1 cut(s) 659
MvaI CCWGG 4 cut(s) 500, 890, 947, 1237
MwoI GCNNNNNNNGC 5 cut(s) 713, 783, 1162, 1301, 1313
NcoI CCATGG 1 cut(s) 966
NdeII GATC 5 cut(s) 133, 177, 702, 721, 1210
NlaIII CATG 7 cut(s) 40, 907, 914, 970, 1051, 1272, 1384
NlaIV GGNNCC 2 cut(s) 222, 237
NmeAIII GCCGAG 1 cut(s) 1182
NmuCI GTSAC 1 cut(s) 316
NsiI ATGCAT 1 cut(s) 562
NspI RCATGY 1 cut(s) 914
PagI TCATGA 1 cut(s) 1380
PctI GAATGC 1 cut(s) 659
PfeI GAWTC 2 cut(s) 161, 1288
PkrI GCNGC 7 cut(s) 113, 625, 694, 952, 1036, 1306, 1309
PleI GAGTC 2 cut(s) 187, 267
PpsI GAGTC 2 cut(s) 187, 267
Psp6I CCWGG 4 cut(s) 498, 888, 945, 1235
PspGI CCWGG 4 cut(s) 498, 888, 945, 1235
PspN4I GGNNCC 2 cut(s) 222, 237
PspPI GGNCC 3 cut(s) 496, 1239, 1375
PstI CTGCAG 1 cut(s) 1306
PstNI CAGNNNCTG 1 cut(s) 1313
PsuI RGATCY 1 cut(s) 133
RsaI GTAC 4 cut(s) 280, 961, 1085, 1331
RsaNI GTAC 4 cut(s) 279, 960, 1084, 1330
RseI CAYNNNNRTG 1 cut(s) 526
SaqAI TTAA 6 cut(s) 119, 150, 443, 582, 873, 1055
SatI GCNGC 7 cut(s) 112, 624, 693, 951, 1035, 1305, 1308
Sau3AI GATC 5 cut(s) 133, 177, 702, 721, 1210
Sau96I GGNCC 3 cut(s) 496, 1239, 1375
SchI GAGTC 2 cut(s) 187, 267
ScrFI CCNGG 4 cut(s) 500, 890, 947, 1237
SduI GDGCHC 3 cut(s) 664, 734, 745
SfaNI GCATC 6 cut(s) 569, 716, 1046, 1162, 1319, 1335
SfcI CTRYAG 2 cut(s) 186, 1302
SinI GGWCC 1 cut(s) 1375
SmiMI CAYNNNNRTG 1 cut(s) 526
SmlI CTYRAG 2 cut(s) 1106, 1251
SmoI CTYRAG 2 cut(s) 1106, 1251
Sse9I AATT 5 cut(s) 27, 263, 366, 399, 862
SsiI CCGC 2 cut(s) 624, 1293
SspI AATATT 2 cut(s) 793, 1069
StyD4I CCNGG 4 cut(s) 498, 888, 945, 1235
StyI CCWWGG 1 cut(s) 966
TaaI ACNGT 9 cut(s) 49, 67, 240, 256, 381, 454, 515, 601, 1187
TaiI ACGT 1 cut(s) 119
TasI AATT 5 cut(s) 27, 263, 366, 399, 862
TatI WGTACW 3 cut(s) 959, 1083, 1329
TauI GCSGC 1 cut(s) 626
TfiI GAWTC 2 cut(s) 161, 1288
Tru1I TTAA 6 cut(s) 119, 150, 443, 582, 873, 1055
Tru9I TTAA 6 cut(s) 119, 150, 443, 582, 873, 1055
TscAI CASTG 5 cut(s) 237, 259, 298, 357, 1047
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 6 cut(s) 111, 692, 950, 1034, 1304, 1307
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 7 cut(s) 53, 527, 531, 920, 994, 1064, 1254
TspGWI ACGGA 1 cut(s) 749
TspRI CASTG 5 cut(s) 237, 259, 298, 357, 1047
VpaK11BI GGWCC 1 cut(s) 1375
XceI RCATGY 1 cut(s) 914
XcmI CCANNNNNNNNNTGG 1 cut(s) 104
Zsp2I ATGCAT 1 cut(s) 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.