Rw5G002720

GPI-anchored protein At1g61900-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0007201
Physical Location & Seq
Forward (+)
2590183 .. 2594250
4068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G002720.1

Sequence Viewer

Length: 1296 bp
ATGCTCATCATTCTCACTTGCCTTTCCTATCAAAATCCTGTCTTGACAATTAGGCAAAAAGATAGCATGTTACCTATGATCTCCCCTACCGCGGCTCCTAAGCCGTTTCTTCCCCTTCTTGCTCCTTCACCATTGGCACCATTCACAAACATTACTGTTCCAAAAATATCAGGACAATGTATGCTGAACTTCACTGCTTCTGAGAGTTTGATGAGTACGACCGCAATTGATTGTTGGTCTGTTTTTGCACCATTGCTGGCAAACGTTATTTGTTGCCCACAGTTGGAAGCTACTATCACAATCCTCATCGGTCAGTCCAGTAAATACACCAATGTGCTTGCTTTAAATGGGACGGCTGCAAAATATTGTCTATCCGATATTAAGCAGATTTTGGTTAGCCAGGGTGCAAATGACACCCTTGCGCAAATATGCTCAGTTCGTTCAACAGATCTCACTGATGCATCTTGCCCCATCATTGATGTTACTGAATTTGAGGAGACAGTGGATACTTCCAAGTTGGTGGCCGCTTGTGAGAAGATAGACCCTGTGAAAGAATGCTGTGCTCAAACATGTCAGAATGCCATATTAGAAGCTGCTACGAGAATTGCTTCAAAAACTTCTGATCTTTTGAGCACAGACGGTGGTCATGCTGTCTTACCTGATCACATAAGTAGAGTCAATGACTGCAAAAATATTGTCCTTCGCTGGCTGGCAAGTAAACTCAATCCTTCTCATGCCAAGGAAGTTCTAAGGGGACTCTCAAATTGCAAGATTAACAAGGTTTGCCCCCTGGTCTTCCCAAATATGAAACATGTTGCACTCGGCTGTGGGGATGAGATAAGCAACCAGACAGCTTGCTGTACGGCCATGGACAGCTATGTTTCCCACTTGCAAAAGCAGAGTTTCATAACCAACTTGCAAGCTTTGGATTGTGCTGCATCACTGGGAATGAAGTTAAGAAAATCAAATATTACCAAAGATGTGTACAGCCTATGTCATATAAGCCTCAAGGATTTCTCCCTTCAAGTTGGAAGTCAAGCATCTGGATGTCTTTTGCCAAGCTTGCCTTCGGATGCAATATTTGAAAGTTCAGGGGTCAGCTTCCTTTGTGATCTGAATGATAACATTCCAGCTCCCTGGCCCACCTCTTCTCAAGGAGCAGCTTCAACATGCAGTAAAGGTGTCAAGATTCCTGCACTTCCTGCAGCAGCATCAGCAGAAAGGGGCAATTACAACAATGTTGTGATGCTTTCTCTGCTCGTTGCTTTTGCAATGGCCCTTTTGATGTTCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

431

Amino Acids

45.99

Weight (kDa)

6.3

Isoelectric Point (pI)

50.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPARK PF19160 57 - 205 5.5e-23 SPARK
At1g61900 PF26584 260 - 333 4.3e-37 At1g61900-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 423
AccB1I GGYRCC 1 cut(s) 136
AccII CGCG 1 cut(s) 92
AciI CCGC 4 cut(s) 90, 92, 222, 525
AclI AACGTT 1 cut(s) 264
AcoI YGGCCR 2 cut(s) 522, 864
AcsI RAATTY 1 cut(s) 488
AfaI GTAC 3 cut(s) 217, 862, 986
AfiI CCNNNNNNNGG 2 cut(s) 91, 283
AflIII ACRYGT 2 cut(s) 569, 811
AgsI TTSAA 5 cut(s) 444, 612, 1025, 1085, 1167
AjnI CCWGG 3 cut(s) 399, 789, 1136
AjuI GAANNNNNNNTTGG 2 cut(s) 1051, 1083
AleI CACNNNNGTG 1 cut(s) 332
AluBI AGCT 9 cut(s) 290, 593, 854, 876, 923, 1062, 1101, 1133, 1163
AluI AGCT 9 cut(s) 290, 593, 854, 876, 923, 1062, 1101, 1133, 1163
Alw21I GWGCWC 2 cut(s) 565, 635
Alw26I GTCTC 1 cut(s) 491
AoxI GGCC 4 cut(s) 522, 864, 1139, 1275
ApeKI GCWGC 6 cut(s) 356, 593, 935, 1160, 1205, 1208
ApoI RAATTY 1 cut(s) 488
ArsI GACNNNNNNTTYG 2 cut(s) 681, 713
Asp700I GAANNNNTTC 1 cut(s) 607
AspLEI GCGC 1 cut(s) 424
AspS9I GGNCC 2 cut(s) 1140, 1276
AsuHPI GGTGA 1 cut(s) 120
BanI GGYRCC 1 cut(s) 136
BbsI GAAGAC 1 cut(s) 787
Bbv12I GWGCWC 2 cut(s) 565, 635
BbvI GCAGC 6 cut(s) 343, 580, 922, 1172, 1217, 1220
BccI CCATC 1 cut(s) 479
BceAI ACGGC 3 cut(s) 88, 369, 879
BciT130I CCWGG 3 cut(s) 401, 791, 1138
BciVI GTATCC 1 cut(s) 499
BclI TGATCA 1 cut(s) 661
BcoDI GTCTC 1 cut(s) 491
BfmI CTRYAG 1 cut(s) 1203
BfuI GTATCC 1 cut(s) 499
BglII AGATCT 1 cut(s) 448
BisI GCNGC 8 cut(s) 93, 357, 525, 594, 936, 1161, 1206, 1209
BlsI GCNGC 8 cut(s) 94, 358, 526, 595, 937, 1162, 1207, 1210
Bme1390I CCNGG 3 cut(s) 401, 791, 1138
BmgT120I GGNCC 2 cut(s) 1140, 1276
BmiI GGNNCC 2 cut(s) 96, 138
BmrFI CCNGG 3 cut(s) 401, 791, 1138
BmrI ACTGGG 1 cut(s) 953
BmsI GCATC 7 cut(s) 448, 470, 947, 1049, 1063, 1220, 1236
BmuI ACTGGG 1 cut(s) 953
BoxI GACNNNNGTC 1 cut(s) 642
BpiI GAAGAC 1 cut(s) 787
Bpu10I CCTNAGC 1 cut(s) 99
BpuEI CTTGAG 2 cut(s) 992, 1137
BsaJI CCNNGG 6 cut(s) 90, 400, 738, 789, 867, 1136
BsaXI ACNNNNNCTCC 2 cut(s) 79, 109
Bsc4I CCNNNNNNNGG 2 cut(s) 91, 283
Bse1I ACTGG 2 cut(s) 318, 948
Bse3DI GCAATG 2 cut(s) 251, 1278
BseBI CCWGG 3 cut(s) 401, 791, 1138
BseDI CCNNGG 6 cut(s) 90, 400, 738, 789, 867, 1136
BseGI GGATG 3 cut(s) 838, 1052, 1078
BseLI CCNNNNNNNGG 2 cut(s) 91, 283
BseMI GCAATG 2 cut(s) 251, 1278
BseMII CTCAG 2 cut(s) 192, 447
BseNI ACTGG 2 cut(s) 318, 948
BseRI GAGGAG 1 cut(s) 509
BseXI GCAGC 6 cut(s) 343, 580, 922, 1172, 1217, 1220
BsgI GTGCAG 1 cut(s) 1179
Bsh1236I CGCG 1 cut(s) 92
Bsh1285I CGRYCG 1 cut(s) 222
BshFI GGCC 4 cut(s) 524, 866, 1141, 1277
BshNI GGYRCC 1 cut(s) 136
BsiEI CGRYCG 1 cut(s) 222
BsiHKAI GWGCWC 2 cut(s) 565, 635
BslFI GGGAC 2 cut(s) 364, 768
BslI CCNNNNNNNGG 2 cut(s) 91, 283
BsmAI GTCTC 1 cut(s) 491
BsmFI GGGAC 2 cut(s) 364, 768
BsmI GAATGC 2 cut(s) 560, 583
BsnI GGCC 4 cut(s) 524, 866, 1141, 1277
Bsp1286I GDGCHC 2 cut(s) 565, 635
Bsp1407I TGTACA 1 cut(s) 984
Bsp143I GATC 5 cut(s) 78, 448, 622, 661, 1111
Bsp19I CCATGG 1 cut(s) 867
BspACI CCGC 4 cut(s) 90, 92, 222, 525
BspANI GGCC 4 cut(s) 524, 866, 1141, 1277
BspCNI CTCAG 2 cut(s) 193, 446
BspFNI CGCG 1 cut(s) 92
BspLI GGNNCC 2 cut(s) 96, 138
BspMAI CTGCAG 1 cut(s) 1207
BspT107I GGYRCC 1 cut(s) 136
BsrDI GCAATG 2 cut(s) 251, 1278
BsrGI TGTACA 1 cut(s) 984
BsrI ACTGG 2 cut(s) 318, 948
BssECI CCNNGG 6 cut(s) 90, 400, 738, 789, 867, 1136
BssMI GATC 5 cut(s) 78, 448, 622, 661, 1111
BssT1I CCWWGG 2 cut(s) 738, 867
Bst2UI CCWGG 3 cut(s) 401, 791, 1138
Bst4CI ACNGT 4 cut(s) 157, 282, 502, 641
Bst6I CTCTTC 1 cut(s) 1153
BstAPI GCANNNNNTGC 1 cut(s) 1202
BstAUI TGTACA 1 cut(s) 984
BstC8I GCNNGC 7 cut(s) 258, 339, 707, 711, 856, 921, 1064
BstDEI CTNAG 4 cut(s) 99, 201, 433, 749
BstDSI CCRYGG 2 cut(s) 90, 867
BstF5I GGATG 3 cut(s) 838, 1052, 1078
BstFNI CGCG 1 cut(s) 92
BstHHI GCGC 1 cut(s) 424
BstKTI GATC 5 cut(s) 81, 451, 625, 664, 1114
BstMAI GTCTC 1 cut(s) 491
BstMBI GATC 5 cut(s) 78, 448, 622, 661, 1111
BstMCI CGRYCG 1 cut(s) 222
BstMWI GCNNNNNNNGC 4 cut(s) 1063, 1202, 1214, 1255
BstNI CCWGG 3 cut(s) 401, 791, 1138
BstNSI RCATGY 4 cut(s) 70, 573, 815, 1173
BstPAI GACNNNNGTC 1 cut(s) 642
BstSCI CCNGG 3 cut(s) 399, 789, 1136
BstSFI CTRYAG 1 cut(s) 1203
BstUI CGCG 1 cut(s) 92
BstV1I GCAGC 6 cut(s) 343, 580, 922, 1172, 1217, 1220
BstV2I GAAGAC 1 cut(s) 787
BstX2I RGATCY 1 cut(s) 448
BstXI CCANNNNNNTGG 2 cut(s) 520, 1137
BstYI RGATCY 1 cut(s) 448
BsuI GTATCC 1 cut(s) 499
BsuRI GGCC 4 cut(s) 524, 866, 1141, 1277
BtgI CCRYGG 2 cut(s) 90, 867
BtsCI GGATG 3 cut(s) 838, 1052, 1078
BtsI GCAGTG 1 cut(s) 192
BtsIMutI CAGTG 4 cut(s) 192, 453, 507, 941
Cac8I GCNNGC 7 cut(s) 258, 339, 707, 711, 856, 921, 1064
CfoI GCGC 1 cut(s) 424
Cfr13I GGNCC 2 cut(s) 1140, 1276
Cfr42I CCGCGG 1 cut(s) 93
Csp6I GTAC 3 cut(s) 216, 861, 985
CviAII CATG 8 cut(s) 67, 570, 647, 734, 812, 868, 1170, 1291
CviQI GTAC 3 cut(s) 216, 861, 985
DdeI CTNAG 4 cut(s) 99, 201, 433, 749
DpnI GATC 5 cut(s) 80, 450, 624, 663, 1113
DpnII GATC 5 cut(s) 78, 448, 622, 661, 1111
DraI TTTAAA 1 cut(s) 345
EaeI YGGCCR 2 cut(s) 522, 864
Eam1104I CTCTTC 1 cut(s) 1153
EarI CTCTTC 1 cut(s) 1153
Eco130I CCWWGG 2 cut(s) 738, 867
EcoRII CCWGG 3 cut(s) 399, 789, 1136
EcoT14I CCWWGG 2 cut(s) 738, 867
EcoT22I ATGCAT 1 cut(s) 463
ErhI CCWWGG 2 cut(s) 738, 867
FaeI CATG 8 cut(s) 70, 573, 650, 737, 815, 871, 1173, 1294
FalI AAGNNNNNCTT 2 cut(s) 1051, 1083
FaqI GGGAC 2 cut(s) 364, 768
FatI CATG 8 cut(s) 66, 569, 646, 733, 811, 867, 1169, 1290
FbaI TGATCA 1 cut(s) 661
Fnu4HI GCNGC 8 cut(s) 93, 357, 525, 594, 936, 1161, 1206, 1209
FokI GGATG 3 cut(s) 845, 1059, 1085
Fsp4HI GCNGC 8 cut(s) 93, 357, 525, 594, 936, 1161, 1206, 1209
FspI TGCGCA 1 cut(s) 423
GlaI GCGC 1 cut(s) 423
GluI GCNGC 8 cut(s) 93, 357, 525, 594, 936, 1161, 1206, 1209
HaeIII GGCC 4 cut(s) 524, 866, 1141, 1277
HhaI GCGC 1 cut(s) 424
Hin1II CATG 8 cut(s) 70, 573, 650, 737, 815, 871, 1173, 1294
Hin6I GCGC 1 cut(s) 422
HinP1I GCGC 1 cut(s) 422
HindIII AAGCTT 2 cut(s) 921, 1060
HinfI GANTC 3 cut(s) 675, 756, 1189
HphI GGTGA 1 cut(s) 120
Hpy166II GTNNAC 2 cut(s) 719, 985
Hpy188I TCNGA 6 cut(s) 202, 376, 576, 622, 1072, 1116
Hpy188III TCNNGA 4 cut(s) 43, 171, 1044, 1186
Hpy8I GTNNAC 2 cut(s) 719, 985
HpyAV CCTTC 6 cut(s) 125, 135, 710, 738, 1031, 1077
HpyCH4III ACNGT 4 cut(s) 157, 282, 502, 641
HpyCH4IV ACGT 1 cut(s) 264
HpyF10VI GCNNNNNNNGC 4 cut(s) 1063, 1202, 1214, 1255
HpyF3I CTNAG 4 cut(s) 99, 201, 433, 749
HpySE526I ACGT 1 cut(s) 264
Hsp92II CATG 8 cut(s) 70, 573, 650, 737, 815, 871, 1173, 1294
HspAI GCGC 1 cut(s) 422
Ksp22I TGATCA 1 cut(s) 661
KspI CCGCGG 1 cut(s) 93
Kzo9I GATC 5 cut(s) 78, 448, 622, 661, 1111
LmnI GCTCC 4 cut(s) 100, 127, 1138, 1157
Lsp1109I GCAGC 6 cut(s) 343, 580, 922, 1172, 1217, 1220
LweI GCATC 7 cut(s) 448, 470, 947, 1049, 1063, 1220, 1236
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 2 cut(s) 69, 481
MalI GATC 5 cut(s) 80, 450, 624, 663, 1113
MboI GATC 5 cut(s) 78, 448, 622, 661, 1111
MboII GAAGA 4 cut(s) 101, 547, 787, 1140
MfeI CAATTG 1 cut(s) 225
MflI RGATCY 1 cut(s) 448
MhlI GDGCHC 2 cut(s) 565, 635
MluCI AATT 6 cut(s) 48, 225, 488, 603, 763, 1228
MlyI GAGTC 2 cut(s) 684, 750
MmeI TCCRAC 2 cut(s) 264, 1009
MnlI CCTC 4 cut(s) 314, 487, 1016, 1156
Mph1103I ATGCAT 1 cut(s) 463
MroXI GAANNNNTTC 1 cut(s) 607
MseI TTAA 4 cut(s) 344, 381, 774, 956
MslI CAYNNNNRTG 2 cut(s) 332, 1045
MspA1I CMGCKG 1 cut(s) 92
MspR9I CCNGG 3 cut(s) 401, 791, 1138
MunI CAATTG 1 cut(s) 225
Mva1269I GAATGC 2 cut(s) 560, 583
MvaI CCWGG 3 cut(s) 401, 791, 1138
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 4 cut(s) 1063, 1202, 1214, 1255
NcoI CCATGG 1 cut(s) 867
NdeII GATC 5 cut(s) 78, 448, 622, 661, 1111
NlaIII CATG 8 cut(s) 70, 573, 650, 737, 815, 871, 1173, 1294
NlaIV GGNNCC 2 cut(s) 96, 138
NmeAIII GCCGAG 1 cut(s) 801
NsbI TGCGCA 1 cut(s) 423
NsiI ATGCAT 1 cut(s) 463
NspI RCATGY 4 cut(s) 70, 573, 815, 1173
OliI CACNNNNGTG 1 cut(s) 332
PciI ACATGT 2 cut(s) 569, 811
PctI GAATGC 2 cut(s) 560, 583
PdmI GAANNNNTTC 1 cut(s) 607
PfeI GAWTC 1 cut(s) 1189
PkrI GCNGC 8 cut(s) 94, 358, 526, 595, 937, 1162, 1207, 1210
PleI GAGTC 2 cut(s) 683, 750
PpsI GAGTC 2 cut(s) 683, 750
PscI ACATGT 2 cut(s) 569, 811
PshAI GACNNNNGTC 1 cut(s) 642
Psp1406I AACGTT 1 cut(s) 264
Psp6I CCWGG 3 cut(s) 399, 789, 1136
PspGI CCWGG 3 cut(s) 399, 789, 1136
PspN4I GGNNCC 2 cut(s) 96, 138
PspPI GGNCC 2 cut(s) 1140, 1276
PstI CTGCAG 1 cut(s) 1207
PsuI RGATCY 1 cut(s) 448
RsaI GTAC 3 cut(s) 217, 862, 986
RsaNI GTAC 3 cut(s) 216, 861, 985
RseI CAYNNNNRTG 2 cut(s) 332, 1045
SacII CCGCGG 1 cut(s) 93
SaqAI TTAA 4 cut(s) 344, 381, 774, 956
SatI GCNGC 8 cut(s) 93, 357, 525, 594, 936, 1161, 1206, 1209
Sau3AI GATC 5 cut(s) 78, 448, 622, 661, 1111
Sau96I GGNCC 2 cut(s) 1140, 1276
SchI GAGTC 2 cut(s) 684, 750
ScrFI CCNGG 3 cut(s) 401, 791, 1138
SduI GDGCHC 2 cut(s) 565, 635
SfaNI GCATC 7 cut(s) 448, 470, 947, 1049, 1063, 1220, 1236
SfcI CTRYAG 1 cut(s) 1203
Sfr303I CCGCGG 1 cut(s) 93
SgrBI CCGCGG 1 cut(s) 93
SmiMI CAYNNNNRTG 2 cut(s) 332, 1045
SmlI CTYRAG 2 cut(s) 1007, 1152
SmoI CTYRAG 2 cut(s) 1007, 1152
Sse9I AATT 6 cut(s) 48, 225, 488, 603, 763, 1228
SsiI CCGC 4 cut(s) 90, 92, 222, 525
SspI AATATT 4 cut(s) 365, 694, 970, 1080
StyD4I CCNGG 3 cut(s) 399, 789, 1136
StyI CCWWGG 2 cut(s) 738, 867
TaaI ACNGT 4 cut(s) 157, 282, 502, 641
TaiI ACGT 1 cut(s) 267
TaqII GACCGA 1 cut(s) 299
TasI AATT 6 cut(s) 48, 225, 488, 603, 763, 1228
TatI WGTACW 1 cut(s) 984
TauI GCSGC 2 cut(s) 95, 527
TfiI GAWTC 1 cut(s) 1189
Tru1I TTAA 4 cut(s) 344, 381, 774, 956
Tru9I TTAA 4 cut(s) 344, 381, 774, 956
TscAI CASTG 4 cut(s) 199, 460, 507, 948
TseI GCWGC 6 cut(s) 356, 593, 935, 1160, 1205, 1208
TspDTI ATGAA 4 cut(s) 821, 895, 965, 1279
TspRI CASTG 4 cut(s) 199, 460, 507, 948
XapI RAATTY 1 cut(s) 488
XceI RCATGY 4 cut(s) 70, 573, 815, 1173
XmnI GAANNNNTTC 1 cut(s) 607
Zsp2I ATGCAT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.