Rorug04G0402600

GPI-anchored protein At1g61900-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0007201
Physical Location & Seq
Forward (+)
56726150 .. 56727333
1184 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0402600.1

Sequence Viewer

Length: 381 bp
ATGAAGAAGAAACCAACTATTAGAAGGTTGGTGAAACCCAGTGGTGACACTGCAACAGCAACTATCGAAGAGGACTACAGGTCTAACAACCCAGCCAGTACCAACTTTCCTCCAGCTTACTTTGGTACCATTCGATGGAATGCTCCTCAGCCAGGCGGCTATCGGCTTCAATTCGTCTTAAGGGATCATGATGCTTCCCCAATCTTTGCGGGTTCCAGATCCATTGGCTATTCCTATGTTCCAGTTGCGGAAGGTAGTGTTGTCAGGGATGGCCTTTATCATGCTCTCATTCTAAATGTCAAGAAGCTTGAAGTTGAGGGTGACTCAAAACTAATCATTGAGGCCATCAACAAGAGATGCATTGTCCCTTGGCGCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

126

Amino Acids

14.09

Weight (kDa)

9.63

Isoelectric Point (pI)

60.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 57 - 119 5.2e-07 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 125
AccB1I GGYRCC 2 cut(s) 125, 372
AccB7I CCANNNNNTGG 1 cut(s) 135
AciI CCGC 3 cut(s) 156, 209, 248
AclWI GGATC 2 cut(s) 192, 213
AcyI GRCGYC 1 cut(s) 373
AfaI GTAC 2 cut(s) 100, 127
AfiI CCNNNNNNNGG 2 cut(s) 135, 152
AflII CTTAAG 1 cut(s) 178
AgsI TTSAA 2 cut(s) 170, 311
AhdI GACNNNNNGTC 1 cut(s) 79
AjnI CCWGG 1 cut(s) 151
AluBI AGCT 2 cut(s) 116, 307
AluI AGCT 2 cut(s) 116, 307
AlwI GGATC 2 cut(s) 192, 213
AoxI GGCC 2 cut(s) 271, 342
Asp718I GGTACC 1 cut(s) 125
AspLEI GCGC 1 cut(s) 375
AsuHPI GGTGA 3 cut(s) 43, 56, 332
BaeI ACNNNNGTAYC 2 cut(s) 109, 142
BanI GGYRCC 2 cut(s) 125, 372
BbvCI CCTCAGC 1 cut(s) 147
BccI CCATC 3 cut(s) 129, 263, 353
BciT130I CCWGG 1 cut(s) 153
BfmI CTRYAG 1 cut(s) 76
BfoI RGCGCY 1 cut(s) 376
BfrI CTTAAG 1 cut(s) 178
BisI GCNGC 1 cut(s) 157
BlsI GCNGC 1 cut(s) 158
Bme1390I CCNGG 1 cut(s) 153
BmeRI GACNNNNNGTC 1 cut(s) 79
BmiI GGNNCC 3 cut(s) 127, 214, 374
BmrFI CCNGG 1 cut(s) 153
BmrI ACTGGG 1 cut(s) 33
BmsI GCATC 2 cut(s) 181, 347
BmuI ACTGGG 1 cut(s) 33
BplI GAGNNNNNCTC 2 cut(s) 308, 340
BpmI CTGGAG 1 cut(s) 96
Bpu10I CCTNAGC 1 cut(s) 147
BsaHI GRCGYC 1 cut(s) 373
BsaJI CCNNGG 1 cut(s) 368
Bsc4I CCNNNNNNNGG 2 cut(s) 135, 152
Bse1I ACTGG 3 cut(s) 39, 96, 242
BseBI CCWGG 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 368
BseGI GGATG 1 cut(s) 274
BseLI CCNNNNNNNGG 2 cut(s) 135, 152
BseMII CTCAG 1 cut(s) 161
BseNI ACTGG 3 cut(s) 39, 96, 242
BseRI GAGGAG 1 cut(s) 135
BseYI CCCAGC 1 cut(s) 91
BshFI GGCC 2 cut(s) 273, 344
BshNI GGYRCC 2 cut(s) 125, 372
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 2 cut(s) 135, 152
BsmFI GGGAC 1 cut(s) 350
BsmI GAATGC 1 cut(s) 145
BsnI GGCC 2 cut(s) 273, 344
Bsp143I GATC 2 cut(s) 184, 218
BspACI CCGC 3 cut(s) 156, 209, 248
BspANI GGCC 2 cut(s) 273, 344
BspCNI CTCAG 1 cut(s) 160
BspHI TCATGA 1 cut(s) 187
BspLI GGNNCC 3 cut(s) 127, 214, 374
BspPI GGATC 2 cut(s) 192, 213
BspT107I GGYRCC 2 cut(s) 125, 372
BspTI CTTAAG 1 cut(s) 178
BsrI ACTGG 3 cut(s) 39, 96, 242
BssECI CCNNGG 1 cut(s) 368
BssMI GATC 2 cut(s) 184, 218
BssNI GRCGYC 1 cut(s) 373
BssT1I CCWWGG 1 cut(s) 368
Bst2UI CCWGG 1 cut(s) 153
Bst6I CTCTTC 1 cut(s) 63
BstACI GRCGYC 1 cut(s) 373
BstAFI CTTAAG 1 cut(s) 178
BstDEI CTNAG 1 cut(s) 147
BstENI CCTNNNNNAGG 1 cut(s) 150
BstF5I GGATG 1 cut(s) 274
BstH2I RGCGCY 1 cut(s) 376
BstHHI GCGC 1 cut(s) 375
BstKTI GATC 2 cut(s) 187, 221
BstMBI GATC 2 cut(s) 184, 218
BstNI CCWGG 1 cut(s) 153
BstSCI CCNGG 1 cut(s) 151
BstSFI CTRYAG 1 cut(s) 76
BstX2I RGATCY 1 cut(s) 218
BstYI RGATCY 1 cut(s) 218
BsuRI GGCC 2 cut(s) 273, 344
BtsCI GGATG 1 cut(s) 274
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 2 cut(s) 46, 48
CciI TCATGA 1 cut(s) 187
CfoI GCGC 1 cut(s) 375
Csp6I GTAC 2 cut(s) 99, 126
CviAII CATG 2 cut(s) 188, 281
CviJI RGCY 9 cut(s) 95, 116, 151, 159, 166, 228, 273, 307, 344
CviKI_1 RGCY 9 cut(s) 95, 116, 151, 159, 166, 228, 273, 307, 344
CviQI GTAC 2 cut(s) 99, 126
DdeI CTNAG 1 cut(s) 147
DinI GGCGCC 1 cut(s) 374
DpnI GATC 2 cut(s) 186, 220
DpnII GATC 2 cut(s) 184, 218
DriI GACNNNNNGTC 1 cut(s) 79
Eam1104I CTCTTC 1 cut(s) 63
Eam1105I GACNNNNNGTC 1 cut(s) 79
EarI CTCTTC 1 cut(s) 63
Eco130I CCWWGG 1 cut(s) 368
EcoNI CCTNNNNNAGG 1 cut(s) 150
EcoRII CCWGG 1 cut(s) 151
EcoT14I CCWWGG 1 cut(s) 368
EcoT22I ATGCAT 1 cut(s) 362
EgeI GGCGCC 1 cut(s) 374
EheI GGCGCC 1 cut(s) 374
ErhI CCWWGG 1 cut(s) 368
FaeI CATG 2 cut(s) 191, 284
FaiI YATR 3 cut(s) 189, 237, 282
FaqI GGGAC 1 cut(s) 350
FatI CATG 2 cut(s) 187, 280
FauI CCCGC 1 cut(s) 202
Fnu4HI GCNGC 1 cut(s) 157
FokI GGATG 1 cut(s) 281
Fsp4HI GCNGC 1 cut(s) 157
GlaI GCGC 1 cut(s) 374
GluI GCNGC 1 cut(s) 157
GsaI CCCAGC 1 cut(s) 95
GsuI CTGGAG 1 cut(s) 96
HaeII RGCGCY 1 cut(s) 376
HaeIII GGCC 2 cut(s) 273, 344
HhaI GCGC 1 cut(s) 375
Hin1I GRCGYC 1 cut(s) 373
Hin1II CATG 2 cut(s) 191, 284
Hin6I GCGC 1 cut(s) 373
HinP1I GCGC 1 cut(s) 373
HindIII AAGCTT 1 cut(s) 305
HinfI GANTC 1 cut(s) 323
HphI GGTGA 3 cut(s) 43, 56, 332
Hpy188III TCNNGA 3 cut(s) 188, 216, 301
HpyAV CCTTC 2 cut(s) 18, 245
HpyCH4V TGCA 2 cut(s) 53, 360
HpyF3I CTNAG 1 cut(s) 147
Hsp92I GRCGYC 1 cut(s) 373
Hsp92II CATG 2 cut(s) 191, 284
HspAI GCGC 1 cut(s) 373
KasI GGCGCC 1 cut(s) 372
KpnI GGTACC 1 cut(s) 129
Kzo9I GATC 2 cut(s) 184, 218
LmnI GCTCC 1 cut(s) 148
LweI GCATC 2 cut(s) 181, 347
MaeIII GTNAC 2 cut(s) 44, 320
MalI GATC 2 cut(s) 186, 220
MboI GATC 2 cut(s) 184, 218
MboII GAAGA 3 cut(s) 16, 19, 80
MflI RGATCY 1 cut(s) 218
MluCI AATT 1 cut(s) 170
Mly113I GGCGCC 1 cut(s) 373
MlyI GAGTC 1 cut(s) 317
MnlI CCTC 5 cut(s) 64, 120, 156, 310, 334
Mph1103I ATGCAT 1 cut(s) 362
MseI TTAA 1 cut(s) 179
MspCI CTTAAG 1 cut(s) 178
MspR9I CCNGG 1 cut(s) 153
Mva1269I GAATGC 1 cut(s) 145
MvaI CCWGG 1 cut(s) 153
NarI GGCGCC 1 cut(s) 373
NdeII GATC 2 cut(s) 184, 218
NlaIII CATG 2 cut(s) 191, 284
NlaIV GGNNCC 3 cut(s) 127, 214, 374
NmuCI GTSAC 2 cut(s) 44, 320
NsiI ATGCAT 1 cut(s) 362
PagI TCATGA 1 cut(s) 187
PctI GAATGC 1 cut(s) 145
PflMI CCANNNNNTGG 1 cut(s) 135
PkrI GCNGC 1 cut(s) 158
PleI GAGTC 1 cut(s) 317
PluTI GGCGCC 1 cut(s) 376
PpsI GAGTC 1 cut(s) 317
Psp6I CCWGG 1 cut(s) 151
PspFI CCCAGC 1 cut(s) 91
PspGI CCWGG 1 cut(s) 151
PspN4I GGNNCC 3 cut(s) 127, 214, 374
PsuI RGATCY 1 cut(s) 218
RsaI GTAC 2 cut(s) 100, 127
RsaNI GTAC 2 cut(s) 99, 126
SaqAI TTAA 1 cut(s) 179
SatI GCNGC 1 cut(s) 157
Sau3AI GATC 2 cut(s) 184, 218
SchI GAGTC 1 cut(s) 317
ScrFI CCNGG 1 cut(s) 153
SetI ASST 5 cut(s) 29, 83, 118, 256, 309
SfaNI GCATC 2 cut(s) 181, 347
SfcI CTRYAG 1 cut(s) 76
SfoI GGCGCC 1 cut(s) 374
SmlI CTYRAG 1 cut(s) 178
SmoI CTYRAG 1 cut(s) 178
Sse9I AATT 1 cut(s) 170
SsiI CCGC 3 cut(s) 156, 209, 248
SspDI GGCGCC 1 cut(s) 372
StyD4I CCNGG 1 cut(s) 151
StyI CCWWGG 1 cut(s) 368
TaqI TCGA 2 cut(s) 66, 133
TasI AATT 1 cut(s) 170
TauI GCSGC 1 cut(s) 159
Tru1I TTAA 1 cut(s) 179
Tru9I TTAA 1 cut(s) 179
TscAI CASTG 2 cut(s) 46, 55
TseFI GTSAC 2 cut(s) 44, 320
Tsp45I GTSAC 2 cut(s) 44, 320
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 46, 55
Van91I CCANNNNNTGG 1 cut(s) 135
Vha464I CTTAAG 1 cut(s) 178
XagI CCTNNNNNAGG 1 cut(s) 150
Zsp2I ATGCAT 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.