pycom05g31400

GPI-anchored protein At1g61900-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0007201
Physical Location & Seq
Reverse (-)
31149148 .. 31151836
2689 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g31400.3

Sequence Viewer

Length: 1227 bp
ATGTTACCAATGATCTCTCCTACAGCGACTCCTCAGCCCTTTCTTCCCCTTCTTGCTCCTTCACCATTGGCACCATATACTAATACCACCGTCCCAAAATTATCAGGACTCTGTAGACTAAACTTCAGTGTTGCTGAGAGTTTGATGAGTGTGACCTCAATTGATTGCTGGTCTGTTTTTGCACCATTACTGGCTAATGTAATGTGTTGTCCACAGTTGGAAGCCACTCTCACAATTCTCGTTGGTCAATTCAGTAAAGAGACCAATGTTCTTGCTTTAAATGGAACGACTGCTAAACACTGTCTATCAGATATTGAACAGATTTTGGTGGGCCAGGGTGCAAATGACAGTCTTGCACAAATATGCTCGGTTCATTCATCAAATCTGACTAATGCATCTTGCCCAGTCTTTGATGTTAATGAGTTTGAGGATACAGTTGATACTTCAAAGTTGCTTGCCGCTTGTGAGAAGATTGACCCTGTGAAAGAATGTTGCGCTCAAACTTGTGAGAATGCCATATTAGAGGCTGCTACAAGGATTGCATCGAAAGTTTCTGATCTTTTGAGCACAGATGGTGCTCATGATCTCTTACCTGATCGATTAAGTAAAGTCAATGACTGCAAAAATATTGTCCTCCGGTGGCTGGCAAGTAAACTCAGTCCTTCTCGCGCCAAGGAAGTTCTAAGGGGACTTTCTAATTGCAAGATTAACAAGGCTTGCCCCCTGGTTTTCCCTGACATGAAGCATGTTGCAATGGGTTGTGGGAATGGGATAAGAAACCGGACTGCTTGTTGTACTGCCATGGAGAGCTATGTGGCCCATTTGCAAAAGCAGAGTCTCATAACCAACTTGCAAGCTTTGGATTGTGCTGCATCACTGGGAATGAAGTTAAGAAAATCAAATATTATCGAAGATGTGTATAGCCTATGTCGTATAAGCCTCAAGGATTTCTCTCTTCAAGTTGGAAATCAAGTGTCTGGATGTCTTTTGCCGAGCATGCCTTCCGATGCAACATTTGACAGTTCAGGAGTCAGCTTCCTTTGTGATTTGAATGATAACATTCCAGCTCCATGGCCCACCTCTCAAGGGGCAGCTTCCTCGTGCGGTAGAAATGTCAGGATTCCTGCTCTTCCTGCAGCAGCATCTGCTGAAAGTGGTCTGTACCATGATAATGTGATGCTTTCTCTACTCCTTGCTTCCGGGATGGTCCTTATGATGCTTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

409

Amino Acids

43.5

Weight (kDa)

5.71

Isoelectric Point (pI)

47.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 70
AccI GTMKAC 1 cut(s) 115
AccII CGCG 1 cut(s) 669
AciI CCGC 2 cut(s) 459, 1104
AcuI CTGAAG 1 cut(s) 109
AfaI GTAC 2 cut(s) 796, 1163
AfiI CCNNNNNNNGG 2 cut(s) 643, 1086
AgsI TTSAA 4 cut(s) 317, 447, 959, 1051
AjnI CCWGG 2 cut(s) 333, 723
AluBI AGCT 5 cut(s) 810, 857, 1035, 1067, 1094
AluI AGCT 5 cut(s) 810, 857, 1035, 1067, 1094
Alw21I GWGCWC 2 cut(s) 569, 580
Alw26I GTCTC 2 cut(s) 254, 842
AlwNI CAGNNNCTG 1 cut(s) 1145
AoxI GGCC 3 cut(s) 331, 816, 1073
ApeKI GCWGC 5 cut(s) 527, 869, 1091, 1136, 1139
ArsI GACNNNNNNTTYG 4 cut(s) 615, 647, 820, 852
AspLEI GCGC 2 cut(s) 497, 671
AspS9I GGNCC 4 cut(s) 331, 817, 1074, 1207
AsuC2I CCSGG 1 cut(s) 1201
AsuHPI GGTGA 1 cut(s) 54
AvaII GGWCC 1 cut(s) 1207
BanI GGYRCC 1 cut(s) 70
BauI CACGAG 1 cut(s) 1099
Bbv12I GWGCWC 2 cut(s) 569, 580
BbvCI CCTCAGC 1 cut(s) 33
BbvI GCAGC 5 cut(s) 514, 856, 1103, 1148, 1151
BccI CCATC 2 cut(s) 566, 1198
BcgI CGANNNNNNTGC 2 cut(s) 1080, 1114
BciT130I CCWGG 2 cut(s) 335, 725
BciVI GTATCC 1 cut(s) 424
BcnI CCSGG 1 cut(s) 1201
BcoDI GTCTC 2 cut(s) 254, 842
BfmI CTRYAG 3 cut(s) 21, 112, 1134
BfuI GTATCC 1 cut(s) 424
BisI GCNGC 6 cut(s) 459, 528, 870, 1092, 1137, 1140
BlsI GCNGC 6 cut(s) 460, 529, 871, 1093, 1138, 1141
Bme1390I CCNGG 3 cut(s) 335, 725, 1201
Bme18I GGWCC 1 cut(s) 1207
BmgT120I GGNCC 4 cut(s) 331, 817, 1074, 1207
BmiI GGNNCC 1 cut(s) 72
BmrFI CCNGG 3 cut(s) 335, 725, 1201
BmrI ACTGGG 2 cut(s) 398, 887
BmsI GCATC 7 cut(s) 404, 551, 881, 997, 1151, 1167, 1206
BmuI ACTGGG 2 cut(s) 398, 887
Bpu10I CCTNAGC 1 cut(s) 33
BpuEI CTTGAG 2 cut(s) 926, 1068
BpuMI CCSGG 1 cut(s) 1201
Bsa29I ATCGAT 1 cut(s) 598
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 5 cut(s) 334, 672, 723, 801, 1070
BsaWI WCCGGW 2 cut(s) 636, 780
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bsc4I CCNNNNNNNGG 2 cut(s) 643, 1086
Bse1I ACTGG 3 cut(s) 195, 404, 882
Bse3DI GCAATG 1 cut(s) 759
BseBI CCWGG 2 cut(s) 335, 725
BseCI ATCGAT 1 cut(s) 598
BseDI CCNNGG 5 cut(s) 334, 672, 723, 801, 1070
BseGI GGATG 2 cut(s) 986, 1209
BseLI CCNNNNNNNGG 2 cut(s) 643, 1086
BseMI GCAATG 1 cut(s) 759
BseMII CTCAG 3 cut(s) 47, 126, 670
BseNI ACTGG 3 cut(s) 195, 404, 882
BseRI GAGGAG 1 cut(s) 21
BseXI GCAGC 5 cut(s) 514, 856, 1103, 1148, 1151
Bsh1236I CGCG 1 cut(s) 669
BshFI GGCC 3 cut(s) 333, 818, 1075
BshNI GGYRCC 1 cut(s) 70
BshVI ATCGAT 1 cut(s) 598
BsiHKAI GWGCWC 2 cut(s) 569, 580
BsiSI CCGG 3 cut(s) 637, 781, 1200
BslFI GGGAC 2 cut(s) 77, 702
BslI CCNNNNNNNGG 2 cut(s) 643, 1086
BsmAI GTCTC 2 cut(s) 254, 842
BsmFI GGGAC 2 cut(s) 77, 702
BsmI GAATGC 1 cut(s) 517
BsnI GGCC 3 cut(s) 333, 818, 1075
Bso31I GGTCTC 1 cut(s) 254
Bsp1286I GDGCHC 2 cut(s) 569, 580
Bsp143I GATC 4 cut(s) 12, 556, 583, 595
Bsp19I CCATGG 2 cut(s) 801, 1070
BspACI CCGC 2 cut(s) 459, 1104
BspANI GGCC 3 cut(s) 333, 818, 1075
BspCNI CTCAG 3 cut(s) 46, 127, 669
BspDI ATCGAT 1 cut(s) 598
BspFNI CGCG 1 cut(s) 669
BspHI TCATGA 1 cut(s) 580
BspLI GGNNCC 1 cut(s) 72
BspMAI CTGCAG 1 cut(s) 1138
BspQI GCTCTTC 1 cut(s) 1134
BspT107I GGYRCC 1 cut(s) 70
BspTNI GGTCTC 1 cut(s) 254
BsrDI GCAATG 1 cut(s) 759
BsrI ACTGG 3 cut(s) 195, 404, 882
BssECI CCNNGG 5 cut(s) 334, 672, 723, 801, 1070
BssMI GATC 4 cut(s) 12, 556, 583, 595
BssSI CACGAG 1 cut(s) 1099
BssT1I CCWWGG 3 cut(s) 672, 801, 1070
Bst2BI CACGAG 1 cut(s) 1099
Bst2UI CCWGG 2 cut(s) 335, 725
Bst4CI ACNGT 6 cut(s) 91, 216, 302, 350, 436, 1022
Bst6I CTCTTC 2 cut(s) 960, 1134
BstAPI GCANNNNNTGC 1 cut(s) 1145
BstC8I GCNNGC 5 cut(s) 456, 645, 718, 855, 998
BstDEI CTNAG 4 cut(s) 33, 135, 656, 683
BstDSI CCRYGG 2 cut(s) 801, 1070
BstF5I GGATG 2 cut(s) 986, 1209
BstFNI CGCG 1 cut(s) 669
BstHHI GCGC 2 cut(s) 497, 671
BstKTI GATC 4 cut(s) 15, 559, 586, 598
BstMAI GTCTC 2 cut(s) 254, 842
BstMBI GATC 4 cut(s) 12, 556, 583, 595
BstMWI GCNNNNNNNGC 3 cut(s) 997, 1133, 1145
BstNI CCWGG 2 cut(s) 335, 725
BstNSI RCATGY 2 cut(s) 749, 1000
BstSCI CCNGG 3 cut(s) 333, 723, 1199
BstSFI CTRYAG 3 cut(s) 21, 112, 1134
BstUI CGCG 1 cut(s) 669
BstV1I GCAGC 5 cut(s) 514, 856, 1103, 1148, 1151
BstXI CCANNNNNNTGG 1 cut(s) 1071
Bsu15I ATCGAT 1 cut(s) 598
BsuI GTATCC 1 cut(s) 424
BsuRI GGCC 3 cut(s) 333, 818, 1075
BsuTUI ATCGAT 1 cut(s) 598
BtgI CCRYGG 2 cut(s) 801, 1070
BtsCI GGATG 2 cut(s) 986, 1209
BtsIMutI CAGTG 3 cut(s) 133, 298, 875
Cac8I GCNNGC 5 cut(s) 456, 645, 718, 855, 998
CaiI CAGNNNCTG 1 cut(s) 1145
CciI TCATGA 1 cut(s) 580
CfoI GCGC 2 cut(s) 497, 671
Cfr13I GGNCC 4 cut(s) 331, 817, 1074, 1207
ClaI ATCGAT 1 cut(s) 598
Csp6I GTAC 2 cut(s) 795, 1162
CviAII CATG 7 cut(s) 581, 739, 746, 802, 997, 1071, 1166
CviQI GTAC 2 cut(s) 795, 1162
DdeI CTNAG 4 cut(s) 33, 135, 656, 683
DpnI GATC 4 cut(s) 14, 558, 585, 597
DpnII GATC 4 cut(s) 12, 556, 583, 595
DraI TTTAAA 1 cut(s) 279
Eam1104I CTCTTC 2 cut(s) 960, 1134
EarI CTCTTC 2 cut(s) 960, 1134
Eco130I CCWWGG 3 cut(s) 672, 801, 1070
Eco31I GGTCTC 1 cut(s) 254
Eco47I GGWCC 1 cut(s) 1207
Eco57I CTGAAG 1 cut(s) 109
EcoRII CCWGG 2 cut(s) 333, 723
EcoT14I CCWWGG 3 cut(s) 672, 801, 1070
EcoT22I ATGCAT 1 cut(s) 397
ErhI CCWWGG 3 cut(s) 672, 801, 1070
FaeI CATG 7 cut(s) 584, 742, 749, 805, 1000, 1074, 1169
FaqI GGGAC 2 cut(s) 77, 702
FatI CATG 7 cut(s) 580, 738, 745, 801, 996, 1070, 1165
FblI GTMKAC 1 cut(s) 115
Fnu4HI GCNGC 6 cut(s) 459, 528, 870, 1092, 1137, 1140
FokI GGATG 2 cut(s) 993, 1216
Fsp4HI GCNGC 6 cut(s) 459, 528, 870, 1092, 1137, 1140
GlaI GCGC 2 cut(s) 496, 670
GluI GCNGC 6 cut(s) 459, 528, 870, 1092, 1137, 1140
HaeIII GGCC 3 cut(s) 333, 818, 1075
HapII CCGG 3 cut(s) 637, 781, 1200
HhaI GCGC 2 cut(s) 497, 671
Hin1II CATG 7 cut(s) 584, 742, 749, 805, 1000, 1074, 1169
Hin6I GCGC 2 cut(s) 495, 669
HinP1I GCGC 2 cut(s) 495, 669
HindIII AAGCTT 1 cut(s) 855
HinfI GANTC 5 cut(s) 28, 108, 835, 1029, 1120
HpaII CCGG 3 cut(s) 637, 781, 1200
HphI GGTGA 1 cut(s) 54
Hpy166II GTNNAC 3 cut(s) 116, 212, 653
Hpy188I TCNGA 4 cut(s) 310, 387, 556, 1006
Hpy188III TCNNGA 5 cut(s) 105, 581, 978, 1026, 1117
Hpy8I GTNNAC 3 cut(s) 116, 212, 653
HpyAV CCTTC 4 cut(s) 59, 69, 672, 1011
HpyCH4III ACNGT 6 cut(s) 91, 216, 302, 350, 436, 1022
HpyF10VI GCNNNNNNNGC 3 cut(s) 997, 1133, 1145
HpyF3I CTNAG 4 cut(s) 33, 135, 656, 683
Hsp92II CATG 7 cut(s) 584, 742, 749, 805, 1000, 1074, 1169
HspAI GCGC 2 cut(s) 495, 669
Kzo9I GATC 4 cut(s) 12, 556, 583, 595
LguI GCTCTTC 1 cut(s) 1134
LmnI GCTCC 2 cut(s) 61, 1072
Lsp1109I GCAGC 5 cut(s) 514, 856, 1103, 1148, 1151
LweI GCATC 7 cut(s) 404, 551, 881, 997, 1151, 1167, 1206
MaeIII GTNAC 2 cut(s) 3, 151
MalI GATC 4 cut(s) 14, 558, 585, 597
MboI GATC 4 cut(s) 12, 556, 583, 595
MboII GAAGA 5 cut(s) 35, 481, 923, 947, 1121
MfeI CAATTG 1 cut(s) 159
MhlI GDGCHC 2 cut(s) 569, 580
MluCI AATT 5 cut(s) 98, 159, 234, 248, 697
MlyI GAGTC 4 cut(s) 22, 102, 844, 1038
MmeI TCCRAC 2 cut(s) 198, 943
MnlI CCTC 8 cut(s) 42, 166, 421, 517, 644, 950, 1090, 1108
Mph1103I ATGCAT 1 cut(s) 397
MseI TTAA 5 cut(s) 278, 417, 602, 708, 890
MslI CAYNNNNRTG 2 cut(s) 361, 1170
MspI CCGG 3 cut(s) 637, 781, 1200
MspR9I CCNGG 3 cut(s) 335, 725, 1201
MunI CAATTG 1 cut(s) 159
Mva1269I GAATGC 1 cut(s) 517
MvaI CCWGG 2 cut(s) 335, 725
MvnI CGCG 1 cut(s) 669
MwoI GCNNNNNNNGC 3 cut(s) 997, 1133, 1145
NciI CCSGG 1 cut(s) 1201
NcoI CCATGG 2 cut(s) 801, 1070
NdeII GATC 4 cut(s) 12, 556, 583, 595
NlaIII CATG 7 cut(s) 584, 742, 749, 805, 1000, 1074, 1169
NlaIV GGNNCC 1 cut(s) 72
NmeAIII GCCGAG 1 cut(s) 1017
NmuCI GTSAC 1 cut(s) 151
NsiI ATGCAT 1 cut(s) 397
NspI RCATGY 2 cut(s) 749, 1000
PaeI GCATGC 1 cut(s) 1000
PagI TCATGA 1 cut(s) 580
PciSI GCTCTTC 1 cut(s) 1134
PctI GAATGC 1 cut(s) 517
PfeI GAWTC 1 cut(s) 1120
PfoI TCCNGGA 1 cut(s) 1199
PkrI GCNGC 6 cut(s) 460, 529, 871, 1093, 1138, 1141
PleI GAGTC 4 cut(s) 22, 102, 843, 1037
PpsI GAGTC 4 cut(s) 22, 102, 843, 1037
Psp6I CCWGG 2 cut(s) 333, 723
PspGI CCWGG 2 cut(s) 333, 723
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 4 cut(s) 331, 817, 1074, 1207
PstI CTGCAG 1 cut(s) 1138
PstNI CAGNNNCTG 1 cut(s) 1145
RsaI GTAC 2 cut(s) 796, 1163
RsaNI GTAC 2 cut(s) 795, 1162
RseI CAYNNNNRTG 2 cut(s) 361, 1170
SapI GCTCTTC 1 cut(s) 1134
SaqAI TTAA 5 cut(s) 278, 417, 602, 708, 890
SatI GCNGC 6 cut(s) 459, 528, 870, 1092, 1137, 1140
Sau3AI GATC 4 cut(s) 12, 556, 583, 595
Sau96I GGNCC 4 cut(s) 331, 817, 1074, 1207
SchI GAGTC 4 cut(s) 22, 102, 844, 1038
ScrFI CCNGG 3 cut(s) 335, 725, 1201
SduI GDGCHC 2 cut(s) 569, 580
SetI ASST 8 cut(s) 158, 595, 812, 859, 1037, 1069, 1082, 1096
SfaNI GCATC 7 cut(s) 404, 551, 881, 997, 1151, 1167, 1206
SfcI CTRYAG 3 cut(s) 21, 112, 1134
SinI GGWCC 1 cut(s) 1207
SmiMI CAYNNNNRTG 2 cut(s) 361, 1170
SmlI CTYRAG 2 cut(s) 941, 1083
SmoI CTYRAG 2 cut(s) 941, 1083
SphI GCATGC 1 cut(s) 1000
Sse9I AATT 5 cut(s) 98, 159, 234, 248, 697
SsiI CCGC 2 cut(s) 459, 1104
SspI AATATT 2 cut(s) 628, 904
StyD4I CCNGG 3 cut(s) 333, 723, 1199
StyI CCWWGG 3 cut(s) 672, 801, 1070
TaaI ACNGT 6 cut(s) 91, 216, 302, 350, 436, 1022
TaqI TCGA 3 cut(s) 545, 598, 909
TasI AATT 5 cut(s) 98, 159, 234, 248, 697
TatI WGTACW 1 cut(s) 794
TauI GCSGC 1 cut(s) 461
TfiI GAWTC 1 cut(s) 1120
Tru1I TTAA 5 cut(s) 278, 417, 602, 708, 890
Tru9I TTAA 5 cut(s) 278, 417, 602, 708, 890
TscAI CASTG 3 cut(s) 133, 305, 882
TseFI GTSAC 1 cut(s) 151
TseI GCWGC 5 cut(s) 527, 869, 1091, 1136, 1139
Tsp45I GTSAC 1 cut(s) 151
TspDTI ATGAA 4 cut(s) 362, 366, 755, 899
TspRI CASTG 3 cut(s) 133, 305, 882
VpaK11BI GGWCC 1 cut(s) 1207
XceI RCATGY 2 cut(s) 749, 1000
XmiI GTMKAC 1 cut(s) 115
Zsp2I ATGCAT 1 cut(s) 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.