Rorug04G0402500

GPI-anchored protein At1g61900-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003392
Physical Location & Seq
Forward (+)
56717166 .. 56723018
5853 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0402500.1

Sequence Viewer

Length: 1443 bp
ATGCAAGAAATCAAGAAGAAGCTTGGCAATGAATATTCCGATTCCAAGAAGAAGGAGCGCCATATCGTCACATGGACTCAAGAGGAAGATGATACACTGCGAGACCAAATTAGCATTCATGGAACGGAGAACTGGGCTATTATTGCGTCTAAATTCAAGGACAAAACGACGAGACAGTGCAGGAGAAGATGGTACACATATTTGAACTCGGACTTCAAGAAGGGAGGATGGTCACCAGAGGAAGACCTGCTTTTATGTGAGGCTCAGAAGATATTTGGTAACAGATGGACCGAAATAGCGAAGGTGGTTTCAGGAAGAACTGACAATGCTGTGAAAAACCGGTTTTCCACTTTGTGCAAGAAGAGAGCAAAATATGAGGCCTTAGCGAAAGAGAATGCCACTTCGTATATGAATCAGAATGACAAAAGGGTCATAATCCGAAATGGCTTCAATACAGATGGAACATCAGAAAATACATCACCTCTTAAGAAGATGAGGAGGAGCCATATCCCTAGTCTCTCGGAAAATTGCAACTATGGTGACAGACGACATGGGCAATGTGGAAAGACTAATCAGCAGCTAAGAGCTCCATTTACTGTGTTGATTCAGAATATCCACAATGTAAACAACCTGCAAGATCAGCATAATGTCAATATCAGTAAGGAAACCCCTAGGAATGCACAAACTAACAAGTTTCAAGGCTCATTTCTCAAGAAGGATGATCCAAAGATAACTGCTTTGATGCAGCAAGCAGAATTGCTCAGCTCATTGGCTATGAAGGTTAATACAGATAACACAGATCAGAGTCTTGAAAATGCTTGGAAGGTTCTCCAAGATTTCTTGAATCAAAGCAAAGAGAGTGACATCCTCAGCTATGGAATGAGTGATTTTGATTTTCAACTCGAAGATCTTAAATATCTATTAGAGGACTTACGGAGTACTAGTGAGGGAGGTCGACCATCATGGCGGCAACCAGATCTCTATGATGAGTCTCCAGGAAGCTCTGAATACAGTACAGGGTCAACTCTTCTGTCCCAAACAGAGTGTTATAAAATGGAACAGATTGAAGCTGAAATAGGTACAATAACTCAGGACATTCCACCCGAGTTGCAAACAATTCACATTGGAGAACAAAATAATATTGGCGAACTTGAGAAAGGGATTGTTTCCAATGGAACTAGAAAGCAAGAGATACTTCCATCTTGCGATGAAGCAACAAACGATGATGCAGTTGTTTCTGCATTGTCGAGTACAGAGTTCAGTTCCCCTATTCAAGTTACTCCAATGTTCAGATCCTTGGCAGCAGGAATTCCCAGCCCAAAGTTTTCTGACAGTGAGAGGAGCTTCTTGCTCAAAACGCTTGGAGCGGATTCCCCTTTTCCCAACCCAAGCACCAATTCACAAACACCACTCTGCAAACGAGCCCTCCTCCAAAGTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

480

Amino Acids

54.39

Weight (kDa)

6.25

Isoelectric Point (pI)

61.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 24 - 68 9.9e-14 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 25 - 84 2.8e-14 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 74 - 117 1.3e-13 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 77 - 124 1.7e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1050
AasI GACNNNNNNGTC 1 cut(s) 428
Acc36I ACCTGC 2 cut(s) 255, 639
AccBSI CCGCTC 1 cut(s) 1367
AccI GTMKAC 1 cut(s) 955
AciI CCGC 2 cut(s) 967, 1367
AclWI GGATC 2 cut(s) 716, 1287
AcsI RAATTY 2 cut(s) 152, 1308
AdeI CACNNNGTG 1 cut(s) 354
AfaI GTAC 5 cut(s) 194, 940, 1015, 1081, 1252
AflII CTTAAG 1 cut(s) 485
AgeI ACCGGT 1 cut(s) 339
AhlI ACTAGT 1 cut(s) 941
AjnI CCWGG 1 cut(s) 994
AjuI GAANNNNNNNTTGG 4 cut(s) 99, 131, 1381, 1413
AluBI AGCT 8 cut(s) 22, 580, 587, 765, 873, 1002, 1070, 1344
AluI AGCT 8 cut(s) 22, 580, 587, 765, 873, 1002, 1070, 1344
Alw21I GWGCWC 1 cut(s) 589
Alw26I GTCTC 4 cut(s) 96, 166, 521, 996
AlwI GGATC 2 cut(s) 716, 1287
Ama87I CYCGRG 1 cut(s) 1103
AoxI GGCC 1 cut(s) 378
ApeKI GCWGC 3 cut(s) 577, 745, 1301
ApoI RAATTY 2 cut(s) 152, 1308
AsiGI ACCGGT 1 cut(s) 339
AspA2I CCTAGG 1 cut(s) 671
AspLEI GCGC 1 cut(s) 60
AspS9I GGNCC 1 cut(s) 288
AsuHPI GGTGA 3 cut(s) 225, 471, 551
AvaI CYCGRG 1 cut(s) 1103
AvaII GGWCC 1 cut(s) 288
AvrII CCTAGG 1 cut(s) 671
BanII GRGCYC 2 cut(s) 589, 1426
BbsI GAAGAC 1 cut(s) 249
Bbv12I GWGCWC 1 cut(s) 589
BbvCI CCTCAGC 1 cut(s) 869
BbvI GCAGC 3 cut(s) 589, 757, 1313
BccI CCATC 6 cut(s) 183, 222, 279, 452, 967, 1207
BciT130I CCWGG 1 cut(s) 996
BcoDI GTCTC 4 cut(s) 96, 166, 521, 996
BcuI ACTAGT 1 cut(s) 941
BfaI CTAG 4 cut(s) 513, 672, 942, 1179
BfmI CTRYAG 1 cut(s) 1439
BfoI RGCGCY 1 cut(s) 61
BfrI CTTAAG 1 cut(s) 485
BfuAI ACCTGC 2 cut(s) 255, 639
BglII AGATCT 2 cut(s) 907, 976
BisI GCNGC 4 cut(s) 578, 746, 968, 1302
BlnI CCTAGG 1 cut(s) 671
BlpI GCTNAGC 1 cut(s) 761
BlsI GCNGC 4 cut(s) 579, 747, 969, 1303
BmcAI AGTACT 1 cut(s) 940
Bme1390I CCNGG 1 cut(s) 996
Bme18I GGWCC 1 cut(s) 288
BmeT110I CYCGRG 1 cut(s) 1103
BmgT120I GGNCC 1 cut(s) 288
BmiI GGNNCC 1 cut(s) 503
BmrFI CCNGG 1 cut(s) 996
BmrI ACTGGG 1 cut(s) 142
BmsI GCATC 2 cut(s) 732, 1216
BmuI ACTGGG 1 cut(s) 142
BpiI GAAGAC 1 cut(s) 249
BplI GAGNNNNNCTC 1 cut(s) 1413
BpmI CTGGAG 1 cut(s) 978
Bpu10I CCTNAGC 2 cut(s) 382, 869
Bpu1102I GCTNAGC 1 cut(s) 761
BpuEI CTTGAG 3 cut(s) 63, 695, 1172
BsaI GGTCTC 1 cut(s) 96
BsaJI CCNNGG 2 cut(s) 671, 1296
BsaWI WCCGGW 1 cut(s) 339
BsaXI ACNNNNNCTCC 4 cut(s) 928, 958, 1410, 1440
Bse118I RCCGGY 1 cut(s) 339
Bse1I ACTGG 1 cut(s) 137
Bse3DI GCAATG 2 cut(s) 34, 563
BseBI CCWGG 1 cut(s) 996
BseDI CCNNGG 2 cut(s) 671, 1296
BseGI GGATG 3 cut(s) 233, 724, 864
BseMI GCAATG 2 cut(s) 34, 563
BseMII CTCAG 4 cut(s) 278, 775, 883, 1103
BseNI ACTGG 1 cut(s) 137
BseRI GAGGAG 4 cut(s) 511, 514, 1354, 1418
BseXI GCAGC 3 cut(s) 589, 757, 1313
BseYI CCCAGC 1 cut(s) 1313
BsgI GTGCAG 1 cut(s) 199
BshFI GGCC 1 cut(s) 380
BshTI ACCGGT 1 cut(s) 339
BsiHKAI GWGCWC 1 cut(s) 589
BsiHKCI CYCGRG 1 cut(s) 1103
BsiSI CCGG 1 cut(s) 340
BslFI GGGAC 1 cut(s) 1018
BsmAI GTCTC 4 cut(s) 96, 166, 521, 996
BsmFI GGGAC 1 cut(s) 1018
BsmI GAATGC 3 cut(s) 114, 400, 682
BsnI GGCC 1 cut(s) 380
Bso31I GGTCTC 1 cut(s) 96
BsoBI CYCGRG 1 cut(s) 1103
Bsp1286I GDGCHC 2 cut(s) 589, 1426
Bsp143I GATC 6 cut(s) 637, 721, 799, 907, 976, 1292
Bsp1720I GCTNAGC 1 cut(s) 761
BspACI CCGC 2 cut(s) 967, 1367
BspANI GGCC 1 cut(s) 380
BspCNI CTCAG 4 cut(s) 277, 774, 882, 1102
BspLI GGNNCC 1 cut(s) 503
BspMI ACCTGC 2 cut(s) 255, 639
BspPI GGATC 2 cut(s) 716, 1287
BspTI CTTAAG 1 cut(s) 485
BspTNI GGTCTC 1 cut(s) 96
BsrBI CCGCTC 1 cut(s) 1367
BsrDI GCAATG 2 cut(s) 34, 563
BsrFI RCCGGY 1 cut(s) 339
BsrI ACTGG 1 cut(s) 137
BssAI RCCGGY 1 cut(s) 339
BssECI CCNNGG 2 cut(s) 671, 1296
BssMI GATC 6 cut(s) 637, 721, 799, 907, 976, 1292
BssT1I CCWWGG 2 cut(s) 671, 1296
Bst2UI CCWGG 1 cut(s) 996
Bst4CI ACNGT 4 cut(s) 177, 598, 1013, 1334
Bst6I CTCTTC 2 cut(s) 356, 1032
BstAFI CTTAAG 1 cut(s) 485
BstC8I GCNNGC 1 cut(s) 750
BstDEI CTNAG 6 cut(s) 264, 382, 581, 761, 869, 1089
BstEII GGTNACC 1 cut(s) 231
BstF5I GGATG 3 cut(s) 233, 724, 864
BstH2I RGCGCY 1 cut(s) 61
BstHHI GCGC 1 cut(s) 60
BstKTI GATC 6 cut(s) 640, 724, 802, 910, 979, 1295
BstMAI GTCTC 4 cut(s) 96, 166, 521, 996
BstMBI GATC 6 cut(s) 637, 721, 799, 907, 976, 1292
BstMWI GCNNNNNNNGC 3 cut(s) 143, 640, 1357
BstNI CCWGG 1 cut(s) 996
BstPI GGTNACC 1 cut(s) 231
BstSCI CCNGG 1 cut(s) 994
BstSFI CTRYAG 1 cut(s) 1439
BstV1I GCAGC 3 cut(s) 589, 757, 1313
BstV2I GAAGAC 1 cut(s) 249
BstX2I RGATCY 3 cut(s) 907, 976, 1292
BstYI RGATCY 3 cut(s) 907, 976, 1292
BsuRI GGCC 1 cut(s) 380
BtgZI GCGATG 1 cut(s) 1221
BtsCI GGATG 3 cut(s) 233, 724, 864
BtsI GCAGTG 1 cut(s) 95
BtsIMutI CAGTG 3 cut(s) 95, 182, 1339
BveI ACCTGC 2 cut(s) 255, 639
Cac8I GCNNGC 1 cut(s) 750
CfoI GCGC 1 cut(s) 60
Cfr10I RCCGGY 1 cut(s) 339
Cfr13I GGNCC 1 cut(s) 288
CseI GACGC 1 cut(s) 135
Csp6I GTAC 5 cut(s) 193, 939, 1014, 1080, 1251
CspAI ACCGGT 1 cut(s) 339
CspCI CAANNNNNGTGG 2 cut(s) 1089, 1124
CviAII CATG 4 cut(s) 72, 119, 551, 963
CviQI GTAC 5 cut(s) 193, 939, 1014, 1080, 1251
DdeI CTNAG 6 cut(s) 264, 382, 581, 761, 869, 1089
DpnI GATC 6 cut(s) 639, 723, 801, 909, 978, 1294
DpnII GATC 6 cut(s) 637, 721, 799, 907, 976, 1292
DraIII CACNNNGTG 1 cut(s) 354
DrdI GACNNNNNNGTC 1 cut(s) 428
DseDI GACNNNNNNGTC 1 cut(s) 428
Eam1104I CTCTTC 2 cut(s) 356, 1032
EarI CTCTTC 2 cut(s) 356, 1032
Ecl136II GAGCTC 1 cut(s) 587
Eco130I CCWWGG 2 cut(s) 671, 1296
Eco147I AGGCCT 1 cut(s) 380
Eco24I GRGCYC 2 cut(s) 589, 1426
Eco31I GGTCTC 1 cut(s) 96
Eco47I GGWCC 1 cut(s) 288
Eco53kI GAGCTC 1 cut(s) 587
Eco88I CYCGRG 1 cut(s) 1103
Eco91I GGTNACC 1 cut(s) 231
EcoICRI GAGCTC 1 cut(s) 587
EcoO65I GGTNACC 1 cut(s) 231
EcoRI GAATTC 1 cut(s) 1308
EcoRII CCWGG 1 cut(s) 994
EcoT14I CCWWGG 2 cut(s) 671, 1296
EcoT38I GRGCYC 2 cut(s) 589, 1426
ErhI CCWWGG 2 cut(s) 671, 1296
FaeI CATG 4 cut(s) 75, 122, 554, 966
FalI AAGNNNNNCTT 4 cut(s) 234, 266, 1179, 1211
FaqI GGGAC 1 cut(s) 1018
FatI CATG 4 cut(s) 71, 118, 550, 962
FblI GTMKAC 1 cut(s) 955
Fnu4HI GCNGC 4 cut(s) 578, 746, 968, 1302
FokI GGATG 3 cut(s) 240, 731, 851
FriOI GRGCYC 2 cut(s) 589, 1426
Fsp4HI GCNGC 4 cut(s) 578, 746, 968, 1302
FspBI CTAG 4 cut(s) 513, 672, 942, 1179
GlaI GCGC 1 cut(s) 59
GluI GCNGC 4 cut(s) 578, 746, 968, 1302
GsaI CCCAGC 1 cut(s) 1317
GsuI CTGGAG 1 cut(s) 978
HaeII RGCGCY 1 cut(s) 61
HaeIII GGCC 1 cut(s) 380
HapII CCGG 1 cut(s) 340
HgaI GACGC 1 cut(s) 135
HhaI GCGC 1 cut(s) 60
Hin1II CATG 4 cut(s) 75, 122, 554, 966
Hin6I GCGC 1 cut(s) 58
HinP1I GCGC 1 cut(s) 58
HincII GTYRAC 2 cut(s) 956, 1023
HindII GTYRAC 2 cut(s) 956, 1023
HindIII AAGCTT 1 cut(s) 20
HinfI GANTC 8 cut(s) 41, 76, 412, 604, 805, 844, 989, 1370
HpaII CCGG 1 cut(s) 340
HphI GGTGA 3 cut(s) 225, 471, 551
Hpy166II GTNNAC 4 cut(s) 195, 625, 956, 1023
Hpy188III TCNNGA 8 cut(s) 13, 80, 217, 312, 712, 809, 841, 1091
Hpy8I GTNNAC 4 cut(s) 195, 625, 956, 1023
Hpy99I CGWCG 1 cut(s) 172
HpyAV CCTTC 6 cut(s) 46, 214, 295, 709, 772, 817
HpyCH4III ACNGT 4 cut(s) 177, 598, 1013, 1334
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 640, 1357
HpyF3I CTNAG 6 cut(s) 264, 382, 581, 761, 869, 1089
Hsp92II CATG 4 cut(s) 75, 122, 554, 966
HspAI GCGC 1 cut(s) 58
Kzo9I GATC 6 cut(s) 637, 721, 799, 907, 976, 1292
LmnI GCTCC 5 cut(s) 55, 501, 592, 1341, 1364
Lsp1109I GCAGC 3 cut(s) 589, 757, 1313
LweI GCATC 2 cut(s) 732, 1216
MaeI CTAG 4 cut(s) 513, 672, 942, 1179
MaeIII GTNAC 6 cut(s) 67, 231, 278, 539, 860, 1276
MalI GATC 6 cut(s) 639, 723, 801, 909, 978, 1294
MbiI CCGCTC 1 cut(s) 1367
MboI GATC 6 cut(s) 637, 721, 799, 907, 976, 1292
MflI RGATCY 3 cut(s) 907, 976, 1292
MhlI GDGCHC 2 cut(s) 589, 1426
MluCI AATT 7 cut(s) 108, 152, 526, 755, 1116, 1308, 1396
MlyI GAGTC 3 cut(s) 70, 814, 998
MseI TTAA 3 cut(s) 486, 783, 912
MspCI CTTAAG 1 cut(s) 485
MspI CCGG 1 cut(s) 340
MspR9I CCNGG 1 cut(s) 996
Mva1269I GAATGC 3 cut(s) 114, 400, 682
MvaI CCWGG 1 cut(s) 996
MwoI GCNNNNNNNGC 3 cut(s) 143, 640, 1357
NdeII GATC 6 cut(s) 637, 721, 799, 907, 976, 1292
NlaIII CATG 4 cut(s) 75, 122, 554, 966
NlaIV GGNNCC 1 cut(s) 503
NmuCI GTSAC 4 cut(s) 67, 231, 539, 860
PceI AGGCCT 1 cut(s) 380
PctI GAATGC 3 cut(s) 114, 400, 682
PfeI GAWTC 5 cut(s) 41, 412, 604, 844, 1370
PfoI TCCNGGA 1 cut(s) 994
PinAI ACCGGT 1 cut(s) 339
PkrI GCNGC 4 cut(s) 579, 747, 969, 1303
PleI GAGTC 3 cut(s) 70, 813, 997
PpsI GAGTC 3 cut(s) 70, 813, 997
PsiI TTATAA 1 cut(s) 1050
Psp124BI GAGCTC 1 cut(s) 589
Psp6I CCWGG 1 cut(s) 994
PspEI GGTNACC 1 cut(s) 231
PspFI CCCAGC 1 cut(s) 1313
PspGI CCWGG 1 cut(s) 994
PspN4I GGNNCC 1 cut(s) 503
PspPI GGNCC 1 cut(s) 288
PsuI RGATCY 3 cut(s) 907, 976, 1292
RsaI GTAC 5 cut(s) 194, 940, 1015, 1081, 1252
RsaNI GTAC 5 cut(s) 193, 939, 1014, 1080, 1251
SacI GAGCTC 1 cut(s) 589
SalI GTCGAC 1 cut(s) 954
SaqAI TTAA 3 cut(s) 486, 783, 912
SatI GCNGC 4 cut(s) 578, 746, 968, 1302
Sau3AI GATC 6 cut(s) 637, 721, 799, 907, 976, 1292
Sau96I GGNCC 1 cut(s) 288
ScaI AGTACT 1 cut(s) 940
SchI GAGTC 3 cut(s) 70, 814, 998
ScrFI CCNGG 1 cut(s) 996
SduI GDGCHC 2 cut(s) 589, 1426
SfaNI GCATC 2 cut(s) 732, 1216
SfcI CTRYAG 1 cut(s) 1439
SinI GGWCC 1 cut(s) 288
SmlI CTYRAG 4 cut(s) 78, 485, 710, 1151
SmoI CTYRAG 4 cut(s) 78, 485, 710, 1151
SpeI ACTAGT 1 cut(s) 941
Sse9I AATT 7 cut(s) 108, 152, 526, 755, 1116, 1308, 1396
SseBI AGGCCT 1 cut(s) 380
SsiI CCGC 2 cut(s) 967, 1367
SspI AATATT 2 cut(s) 35, 1141
SspMI CTAG 4 cut(s) 513, 672, 942, 1179
SstI GAGCTC 1 cut(s) 589
StuI AGGCCT 1 cut(s) 380
StyD4I CCNGG 1 cut(s) 994
StyI CCWWGG 2 cut(s) 671, 1296
TaaI ACNGT 4 cut(s) 177, 598, 1013, 1334
TaqI TCGA 3 cut(s) 903, 955, 1247
TaqII GACCGA 1 cut(s) 305
TasI AATT 7 cut(s) 108, 152, 526, 755, 1116, 1308, 1396
TatI WGTACW 3 cut(s) 938, 1013, 1250
TauI GCSGC 1 cut(s) 970
TfiI GAWTC 5 cut(s) 41, 412, 604, 844, 1370
Tru1I TTAA 3 cut(s) 486, 783, 912
Tru9I TTAA 3 cut(s) 486, 783, 912
TscAI CASTG 3 cut(s) 102, 182, 1339
TseFI GTSAC 4 cut(s) 67, 231, 539, 860
TseI GCWGC 3 cut(s) 577, 745, 1301
Tsp45I GTSAC 4 cut(s) 67, 231, 539, 860
TspDTI ATGAA 5 cut(s) 45, 107, 425, 791, 1224
TspGWI ACGGA 2 cut(s) 140, 949
TspRI CASTG 3 cut(s) 102, 182, 1339
Vha464I CTTAAG 1 cut(s) 485
VpaK11BI GGWCC 1 cut(s) 288
XapI RAATTY 2 cut(s) 152, 1308
XmaJI CCTAGG 1 cut(s) 671
XmiI GTMKAC 1 cut(s) 955
XspI CTAG 4 cut(s) 513, 672, 942, 1179
ZrmI AGTACT 1 cut(s) 940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.