FvH4_3g02220

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003392
Physical Location & Seq
Forward (+)
1132001 .. 1132390
390 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g02220.t1

Sequence Viewer

Length: 390 bp
ATGGGGAACTGCGGTTTGAGGCGTGATTCCGCGGCGGTTTGGGCCGACAACAACGACGACGGCTGGGTGAACGTTTGTGAGCTCCACCAGTCAGAGCAGCCGGAAAAGGAGAAGCTTCTCGGTGAGGTGCAGTTTAGATCTTCAGCTTCCTCGTCGGCGTCGTCGTGTACACAAAGACCGATGAGTAGTAGCGGTGGAGATCATCAGGTGAAGATAAAGATGACAAAACGGGAGCTGGAGGAGTTGGTAGAACCACGAGGCGGCGCTATGGACGCGTCGTCGGTGGAGGAATTACTGGCGAGGGCGATCGACGACGGTGATGATGAGCTGGTGGAGGAGCATCAACGGCCATGGAGGCCTGCCCTCCAAAGTATTCCGGAGGTCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

130

Amino Acids

14.25

Weight (kDa)

4.6

Isoelectric Point (pI)

66.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 32, 275
AccIII TCCGGA 1 cut(s) 376
AciI CCGC 6 cut(s) 12, 30, 32, 35, 192, 261
AclI AACGTT 1 cut(s) 72
AcoI YGGCCR 1 cut(s) 347
AcuI CTGAAG 1 cut(s) 126
AcyI GRCGYC 1 cut(s) 158
AfaI GTAC 1 cut(s) 169
AfiI CCNNNNNNNGG 1 cut(s) 260
AflIII ACRYGT 1 cut(s) 273
AhdI GACNNNNNGTC 1 cut(s) 277
AluBI AGCT 5 cut(s) 82, 115, 146, 235, 328
AluI AGCT 5 cut(s) 82, 115, 146, 235, 328
Alw21I GWGCWC 1 cut(s) 84
Aor13HI TCCGGA 1 cut(s) 376
AoxI GGCC 3 cut(s) 42, 347, 356
ApeKI GCWGC 1 cut(s) 97
AspLEI GCGC 1 cut(s) 266
AspS9I GGNCC 1 cut(s) 42
AsuHPI GGTGA 4 cut(s) 79, 134, 220, 329
BanII GRGCYC 1 cut(s) 84
BauI CACGAG 1 cut(s) 255
Bbv12I GWGCWC 1 cut(s) 84
BbvI GCAGC 1 cut(s) 109
BceAI ACGGC 2 cut(s) 76, 362
BfoI RGCGCY 1 cut(s) 267
BglI GCCNNNNNGGC 1 cut(s) 355
BglII AGATCT 1 cut(s) 137
BisI GCNGC 3 cut(s) 33, 98, 262
BlsI GCNGC 3 cut(s) 34, 99, 263
BmeRI GACNNNNNGTC 1 cut(s) 277
BmgT120I GGNCC 1 cut(s) 42
BmsI GCATC 1 cut(s) 349
BpmI CTGGAG 1 cut(s) 257
BsaHI GRCGYC 1 cut(s) 158
BsaJI CCNNGG 2 cut(s) 30, 350
BsaWI WCCGGW 1 cut(s) 376
Bsc4I CCNNNNNNNGG 1 cut(s) 260
Bse1I ACTGG 2 cut(s) 88, 300
BseAI TCCGGA 1 cut(s) 376
BseDI CCNNGG 2 cut(s) 30, 350
BseLI CCNNNNNNNGG 1 cut(s) 260
BseNI ACTGG 2 cut(s) 88, 300
BseRI GAGGAG 2 cut(s) 254, 350
BseXI GCAGC 1 cut(s) 109
BseYI CCCAGC 1 cut(s) 63
BsgI GTGCAG 1 cut(s) 149
Bsh1236I CGCG 2 cut(s) 32, 275
Bsh1285I CGRYCG 1 cut(s) 309
BshFI GGCC 3 cut(s) 44, 349, 358
BsiEI CGRYCG 1 cut(s) 309
BsiHKAI GWGCWC 1 cut(s) 84
BsiSI CCGG 2 cut(s) 101, 377
BslI CCNNNNNNNGG 1 cut(s) 260
BsnI GGCC 3 cut(s) 44, 349, 358
Bsp1286I GDGCHC 1 cut(s) 84
Bsp13I TCCGGA 1 cut(s) 376
Bsp1407I TGTACA 1 cut(s) 167
Bsp143I GATC 3 cut(s) 137, 199, 306
Bsp19I CCATGG 1 cut(s) 350
BspACI CCGC 6 cut(s) 12, 30, 32, 35, 192, 261
BspANI GGCC 3 cut(s) 44, 349, 358
BspEI TCCGGA 1 cut(s) 376
BspFNI CGCG 2 cut(s) 32, 275
BsrGI TGTACA 1 cut(s) 167
BsrI ACTGG 2 cut(s) 88, 300
BssECI CCNNGG 2 cut(s) 30, 350
BssMI GATC 3 cut(s) 137, 199, 306
BssNI GRCGYC 1 cut(s) 158
BssSI CACGAG 1 cut(s) 255
BssT1I CCWWGG 1 cut(s) 350
Bst2BI CACGAG 1 cut(s) 255
Bst4CI ACNGT 1 cut(s) 317
BstACI GRCGYC 1 cut(s) 158
BstAUI TGTACA 1 cut(s) 167
BstC8I GCNNGC 1 cut(s) 360
BstDSI CCRYGG 2 cut(s) 30, 350
BstFNI CGCG 2 cut(s) 32, 275
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 1 cut(s) 266
BstKTI GATC 3 cut(s) 140, 202, 309
BstMBI GATC 3 cut(s) 137, 199, 306
BstMCI CGRYCG 1 cut(s) 309
BstMWI GCNNNNNNNGC 4 cut(s) 41, 272, 346, 355
BstUI CGCG 2 cut(s) 32, 275
BstV1I GCAGC 1 cut(s) 109
BstX2I RGATCY 1 cut(s) 137
BstYI RGATCY 1 cut(s) 137
BsuRI GGCC 3 cut(s) 44, 349, 358
BtgI CCRYGG 2 cut(s) 30, 350
Cac8I GCNNGC 1 cut(s) 360
CfoI GCGC 1 cut(s) 266
Cfr13I GGNCC 1 cut(s) 42
Cfr42I CCGCGG 1 cut(s) 33
CseI GACGC 3 cut(s) 147, 264, 281
Csp6I GTAC 1 cut(s) 168
CviAII CATG 1 cut(s) 351
CviQI GTAC 1 cut(s) 168
DpnI GATC 3 cut(s) 139, 201, 308
DpnII GATC 3 cut(s) 137, 199, 306
DriI GACNNNNNGTC 1 cut(s) 277
EaeI YGGCCR 1 cut(s) 347
Eam1105I GACNNNNNGTC 1 cut(s) 277
Ecl136II GAGCTC 1 cut(s) 82
Eco130I CCWWGG 1 cut(s) 350
Eco147I AGGCCT 1 cut(s) 358
Eco24I GRGCYC 1 cut(s) 84
Eco53kI GAGCTC 1 cut(s) 82
Eco57I CTGAAG 1 cut(s) 126
EcoICRI GAGCTC 1 cut(s) 82
EcoT14I CCWWGG 1 cut(s) 350
EcoT38I GRGCYC 1 cut(s) 84
ErhI CCWWGG 1 cut(s) 350
FaeI CATG 1 cut(s) 354
FaiI YATR 2 cut(s) 269, 352
FatI CATG 1 cut(s) 350
Fnu4HI GCNGC 3 cut(s) 33, 98, 262
FriOI GRGCYC 1 cut(s) 84
Fsp4HI GCNGC 3 cut(s) 33, 98, 262
GlaI GCGC 1 cut(s) 265
GluI GCNGC 3 cut(s) 33, 98, 262
GsaI CCCAGC 1 cut(s) 67
GsuI CTGGAG 1 cut(s) 257
HaeII RGCGCY 1 cut(s) 267
HaeIII GGCC 3 cut(s) 44, 349, 358
HapII CCGG 2 cut(s) 101, 377
HgaI GACGC 3 cut(s) 147, 264, 281
HhaI GCGC 1 cut(s) 266
Hin1I GRCGYC 1 cut(s) 158
Hin1II CATG 1 cut(s) 354
Hin6I GCGC 1 cut(s) 264
HinP1I GCGC 1 cut(s) 264
HindIII AAGCTT 1 cut(s) 113
HinfI GANTC 1 cut(s) 26
HpaII CCGG 2 cut(s) 101, 377
HphI GGTGA 4 cut(s) 79, 134, 220, 329
Hpy166II GTNNAC 3 cut(s) 70, 168, 170
Hpy188I TCNGA 1 cut(s) 94
Hpy188III TCNNGA 1 cut(s) 377
Hpy8I GTNNAC 3 cut(s) 70, 168, 170
Hpy99I CGWCG 9 cut(s) 59, 62, 157, 163, 166, 280, 283, 314, 317
HpyCH4III ACNGT 1 cut(s) 317
HpyCH4IV ACGT 1 cut(s) 72
HpyCH4V TGCA 1 cut(s) 130
HpyF10VI GCNNNNNNNGC 4 cut(s) 41, 272, 346, 355
HpySE526I ACGT 1 cut(s) 72
Hsp92I GRCGYC 1 cut(s) 158
Hsp92II CATG 1 cut(s) 354
HspAI GCGC 1 cut(s) 264
Kpn2I TCCGGA 1 cut(s) 376
KspI CCGCGG 1 cut(s) 33
Kzo9I GATC 3 cut(s) 137, 199, 306
LmnI GCTCC 3 cut(s) 87, 232, 337
LpnPI CCDG 8 cut(s) 49, 101, 114, 191, 221, 281, 314, 372
Lsp1109I GCAGC 1 cut(s) 109
LweI GCATC 1 cut(s) 349
MaeII ACGT 1 cut(s) 72
MalI GATC 3 cut(s) 139, 201, 308
MboI GATC 3 cut(s) 137, 199, 306
MboII GAAGA 2 cut(s) 132, 223
MflI RGATCY 1 cut(s) 137
MhlI GDGCHC 1 cut(s) 84
MluCI AATT 1 cut(s) 290
MluI ACGCGT 1 cut(s) 273
MroI TCCGGA 1 cut(s) 376
MspA1I CMGCKG 1 cut(s) 32
MspI CCGG 2 cut(s) 101, 377
MvnI CGCG 2 cut(s) 32, 275
MwoI GCNNNNNNNGC 4 cut(s) 41, 272, 346, 355
NcoI CCATGG 1 cut(s) 350
NdeII GATC 3 cut(s) 137, 199, 306
NlaIII CATG 1 cut(s) 354
PceI AGGCCT 1 cut(s) 358
PcsI WCGNNNNNNNCGW 2 cut(s) 158, 161
PfeI GAWTC 1 cut(s) 26
PkrI GCNGC 3 cut(s) 34, 99, 263
Ple19I CGATCG 1 cut(s) 309
Psp124BI GAGCTC 1 cut(s) 84
Psp1406I AACGTT 1 cut(s) 72
PspFI CCCAGC 1 cut(s) 63
PspPI GGNCC 1 cut(s) 42
PsuI RGATCY 1 cut(s) 137
PvuI CGATCG 1 cut(s) 309
RsaI GTAC 1 cut(s) 169
RsaNI GTAC 1 cut(s) 168
SacI GAGCTC 1 cut(s) 84
SacII CCGCGG 1 cut(s) 33
SatI GCNGC 3 cut(s) 33, 98, 262
Sau3AI GATC 3 cut(s) 137, 199, 306
Sau96I GGNCC 1 cut(s) 42
SduI GDGCHC 1 cut(s) 84
SetI ASST 9 cut(s) 75, 84, 117, 129, 148, 210, 237, 330, 384
SfaNI GCATC 1 cut(s) 349
SfiI GGCCNNNNNGGCC 1 cut(s) 355
Sfr303I CCGCGG 1 cut(s) 33
SgrBI CCGCGG 1 cut(s) 33
Sse9I AATT 1 cut(s) 290
SseBI AGGCCT 1 cut(s) 358
SsiI CCGC 6 cut(s) 12, 30, 32, 35, 192, 261
SstI GAGCTC 1 cut(s) 84
StuI AGGCCT 1 cut(s) 358
StyI CCWWGG 1 cut(s) 350
TaaI ACNGT 1 cut(s) 317
TaiI ACGT 1 cut(s) 75
TaqI TCGA 2 cut(s) 309, 384
TaqII GACCGA 1 cut(s) 193
TasI AATT 1 cut(s) 290
TatI WGTACW 1 cut(s) 167
TauI GCSGC 2 cut(s) 35, 264
TfiI GAWTC 1 cut(s) 26
TseI GCWGC 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.