MD10G1320500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003392
Physical Location & Seq
Reverse (-)
40222086 .. 40222496
411 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1320500.v1.1.491

Sequence Viewer

Length: 411 bp
ATGGGAAACTGTTTGAGACGCGATTCGGCGATGGTATGGGCCAGAGACGACGACTGGATTGATGTTTCTGAGCTTGAGCCTGATGCTAAAATGGCACACAGCCCAGAAAGGCATAGGCTTCTTGGCGAGATAAGGTCATATTCATCGTCTGCAGCCTCGACACCTTCATTTTCTACTAGCACTAGTAGTACTGGAGGAGATCAAGTGAAGATCAAGATTACTAAGAAGGAGCTGGAGGAGTTGGTAAAAGGAGGAGGAAACATGCAGGGTCTGTCGTCTGTGGAACAGTTATTGGCTCGGCTCAGGATCAACGGCCTTGATGATCATGATCAGTACTATGATGATCGTGATGAGATGGACCATCATCGGCCCTGGAGGCCTGTTCTCCAAAGTATTCCGGAGGTTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

15.37

Weight (kDa)

5.16

Isoelectric Point (pI)

52.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 21
AccIII TCCGGA 1 cut(s) 397
AclWI GGATC 1 cut(s) 314
AfaI GTAC 2 cut(s) 190, 335
AhlI ACTAGT 1 cut(s) 182
AjnI CCWGG 1 cut(s) 371
AluBI AGCT 2 cut(s) 73, 232
AluI AGCT 2 cut(s) 73, 232
Alw26I GTCTC 2 cut(s) 10, 39
AlwI GGATC 1 cut(s) 314
AlwNI CAGNNNCTG 1 cut(s) 271
Aor13HI TCCGGA 1 cut(s) 397
AoxI GGCC 4 cut(s) 39, 313, 368, 377
ApeKI GCWGC 1 cut(s) 152
AspS9I GGNCC 3 cut(s) 39, 358, 369
AvaII GGWCC 1 cut(s) 358
BbvI GCAGC 1 cut(s) 164
BccI CCATC 3 cut(s) 25, 349, 369
BceAI ACGGC 1 cut(s) 328
BciT130I CCWGG 1 cut(s) 373
BclI TGATCA 2 cut(s) 322, 328
BcoDI GTCTC 2 cut(s) 10, 39
BcuI ACTAGT 1 cut(s) 182
BfaI CTAG 2 cut(s) 177, 183
BfmI CTRYAG 1 cut(s) 150
BglI GCCNNNNNGGC 1 cut(s) 376
BisI GCNGC 1 cut(s) 153
BlsI GCNGC 1 cut(s) 154
BmcAI AGTACT 2 cut(s) 190, 335
Bme1390I CCNGG 1 cut(s) 373
Bme18I GGWCC 1 cut(s) 358
BmgT120I GGNCC 3 cut(s) 39, 358, 369
BmrFI CCNGG 1 cut(s) 373
BmsI GCATC 1 cut(s) 73
BpmI CTGGAG 3 cut(s) 213, 254, 394
Bpu10I CCTNAGC 1 cut(s) 302
BpuEI CTTGAG 1 cut(s) 95
BsaBI GATNNNNATC 1 cut(s) 327
BsaJI CCNNGG 1 cut(s) 371
BsaWI WCCGGW 1 cut(s) 397
BsaXI ACNNNNNCTCC 2 cut(s) 189, 219
Bse1I ACTGG 2 cut(s) 59, 196
Bse8I GATNNNNATC 1 cut(s) 327
BseAI TCCGGA 1 cut(s) 397
BseBI CCWGG 1 cut(s) 373
BseDI CCNNGG 1 cut(s) 371
BseJI GATNNNNATC 1 cut(s) 327
BseMII CTCAG 2 cut(s) 60, 316
BseNI ACTGG 2 cut(s) 59, 196
BseRI GAGGAG 3 cut(s) 210, 251, 267
BseXI GCAGC 1 cut(s) 164
Bsh1236I CGCG 1 cut(s) 21
BshFI GGCC 4 cut(s) 41, 315, 370, 379
BsiSI CCGG 1 cut(s) 398
BsmAI GTCTC 2 cut(s) 10, 39
BsmBI CGTCTC 2 cut(s) 10, 39
BsnI GGCC 4 cut(s) 41, 315, 370, 379
Bsp13I TCCGGA 1 cut(s) 397
Bsp143I GATC 6 cut(s) 199, 210, 306, 322, 328, 343
BspANI GGCC 4 cut(s) 41, 315, 370, 379
BspCNI CTCAG 2 cut(s) 61, 315
BspEI TCCGGA 1 cut(s) 397
BspFNI CGCG 1 cut(s) 21
BspHI TCATGA 1 cut(s) 325
BspMAI CTGCAG 1 cut(s) 154
BspPI GGATC 1 cut(s) 314
BsrI ACTGG 2 cut(s) 59, 196
BssECI CCNNGG 1 cut(s) 371
BssMI GATC 6 cut(s) 199, 210, 306, 322, 328, 343
Bst2UI CCWGG 1 cut(s) 373
Bst4CI ACNGT 2 cut(s) 11, 288
BstDEI CTNAG 3 cut(s) 69, 222, 302
BstFNI CGCG 1 cut(s) 21
BstKTI GATC 6 cut(s) 202, 213, 309, 325, 331, 346
BstMAI GTCTC 2 cut(s) 10, 39
BstMBI GATC 6 cut(s) 199, 210, 306, 322, 328, 343
BstMWI GCNNNNNNNGC 2 cut(s) 92, 376
BstNI CCWGG 1 cut(s) 373
BstNSI RCATGY 1 cut(s) 265
BstSCI CCNGG 1 cut(s) 371
BstSFI CTRYAG 1 cut(s) 150
BstUI CGCG 1 cut(s) 21
BstV1I GCAGC 1 cut(s) 164
BsuRI GGCC 4 cut(s) 41, 315, 370, 379
BtgZI GCGATG 1 cut(s) 44
CaiI CAGNNNCTG 1 cut(s) 271
CciI TCATGA 1 cut(s) 325
Cfr13I GGNCC 3 cut(s) 39, 358, 369
CseI GACGC 1 cut(s) 27
Csp6I GTAC 2 cut(s) 189, 334
CviAII CATG 2 cut(s) 262, 326
CviQI GTAC 2 cut(s) 189, 334
DdeI CTNAG 3 cut(s) 69, 222, 302
DpnI GATC 6 cut(s) 201, 212, 308, 324, 330, 345
DpnII GATC 6 cut(s) 199, 210, 306, 322, 328, 343
Eco147I AGGCCT 1 cut(s) 379
Eco47I GGWCC 1 cut(s) 358
EcoRII CCWGG 1 cut(s) 371
Esp3I CGTCTC 2 cut(s) 10, 39
FaeI CATG 2 cut(s) 265, 329
FaiI YATR 6 cut(s) 37, 114, 139, 263, 327, 339
FatI CATG 2 cut(s) 261, 325
FbaI TGATCA 2 cut(s) 322, 328
Fnu4HI GCNGC 1 cut(s) 153
Fsp4HI GCNGC 1 cut(s) 153
FspBI CTAG 2 cut(s) 177, 183
GluI GCNGC 1 cut(s) 153
GsuI CTGGAG 3 cut(s) 213, 254, 394
HaeIII GGCC 4 cut(s) 41, 315, 370, 379
HapII CCGG 1 cut(s) 398
HgaI GACGC 1 cut(s) 27
Hin1II CATG 2 cut(s) 265, 329
HinfI GANTC 1 cut(s) 23
HpaII CCGG 1 cut(s) 398
Hpy188I TCNGA 1 cut(s) 70
Hpy188III TCNNGA 5 cut(s) 214, 304, 326, 347, 398
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 2 cut(s) 174, 220
HpyCH4III ACNGT 2 cut(s) 11, 288
HpyCH4V TGCA 2 cut(s) 152, 265
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 376
HpyF3I CTNAG 3 cut(s) 69, 222, 302
Hsp92II CATG 2 cut(s) 265, 329
Kpn2I TCCGGA 1 cut(s) 397
Ksp22I TGATCA 2 cut(s) 322, 328
Kzo9I GATC 6 cut(s) 199, 210, 306, 322, 328, 343
LmnI GCTCC 1 cut(s) 229
Lsp1109I GCAGC 1 cut(s) 164
LweI GCATC 1 cut(s) 73
MaeI CTAG 2 cut(s) 177, 183
MalI GATC 6 cut(s) 201, 212, 308, 324, 330, 345
MboI GATC 6 cut(s) 199, 210, 306, 322, 328, 343
MboII GAAGA 1 cut(s) 220
MluCI AATT 1 cut(s) 406
MnlI CCTC 7 cut(s) 166, 188, 229, 245, 248, 369, 394
MroI TCCGGA 1 cut(s) 397
MseI TTAA 1 cut(s) 405
MspI CCGG 1 cut(s) 398
MspR9I CCNGG 1 cut(s) 373
MvaI CCWGG 1 cut(s) 373
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 2 cut(s) 92, 376
NdeII GATC 6 cut(s) 199, 210, 306, 322, 328, 343
NlaIII CATG 2 cut(s) 265, 329
NmeAIII GCCGAG 1 cut(s) 277
NspI RCATGY 1 cut(s) 265
PagI TCATGA 1 cut(s) 325
PceI AGGCCT 1 cut(s) 379
PfeI GAWTC 1 cut(s) 23
PkrI GCNGC 1 cut(s) 154
Psp6I CCWGG 1 cut(s) 371
PspGI CCWGG 1 cut(s) 371
PspPI GGNCC 3 cut(s) 39, 358, 369
PstI CTGCAG 1 cut(s) 154
PstNI CAGNNNCTG 1 cut(s) 271
RsaI GTAC 2 cut(s) 190, 335
RsaNI GTAC 2 cut(s) 189, 334
SaqAI TTAA 1 cut(s) 405
SatI GCNGC 1 cut(s) 153
Sau3AI GATC 6 cut(s) 199, 210, 306, 322, 328, 343
Sau96I GGNCC 3 cut(s) 39, 358, 369
ScaI AGTACT 2 cut(s) 190, 335
ScrFI CCNGG 1 cut(s) 373
SetI ASST 5 cut(s) 75, 137, 166, 234, 405
SfaNI GCATC 1 cut(s) 73
SfcI CTRYAG 1 cut(s) 150
SfiI GGCCNNNNNGGCC 1 cut(s) 376
SinI GGWCC 1 cut(s) 358
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
SpeI ACTAGT 1 cut(s) 182
Sse9I AATT 1 cut(s) 406
SseBI AGGCCT 1 cut(s) 379
SspMI CTAG 2 cut(s) 177, 183
StuI AGGCCT 1 cut(s) 379
StyD4I CCNGG 1 cut(s) 371
TaaI ACNGT 2 cut(s) 11, 288
TaqI TCGA 1 cut(s) 158
TasI AATT 1 cut(s) 406
TatI WGTACW 2 cut(s) 188, 333
TfiI GAWTC 1 cut(s) 23
Tru1I TTAA 1 cut(s) 405
Tru9I TTAA 1 cut(s) 405
TseI GCWGC 1 cut(s) 152
TspDTI ATGAA 2 cut(s) 132, 156
VpaK11BI GGWCC 1 cut(s) 358
XceI RCATGY 1 cut(s) 265
XspI CTAG 2 cut(s) 177, 183
ZrmI AGTACT 2 cut(s) 190, 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.