pycom11g19280

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0003392
Physical Location & Seq
Forward (+)
21612342 .. 21613141
800 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g19280.1

Sequence Viewer

Length: 546 bp
ATGGGAAATTGCATAGGGCATGATTCGTCCATGAAATGGGCAGGAGAGGATTGGGGGTGTCTAGCAGATGGAATGAAGGGTGACGATTATCGCGATCAAACGGACGTAAAGATCATGAAGAAGAACAACAAGAAGATTGCAGAAGCGAGCCAGATGGATTCATTTTGTTCTACAACAACAGCAACAAGAAGTAGTAGTAGTAAAGTTGGTGCAGCAACAGAGGTGAAGATCAAGATCACAAAGAAGCAGTTGGAGGAGTTGTTGGGTAAGGTGGAGTATAAGCAAATGTCAGTTCAGCAGGTTTTGGCTCAGCTGATTAGGGTTAGCGATGGCTTTGAGACCCAGCTGCAAAGGTCTTGGAGGCCTGCTCTTCAAAGCATTCCTGAGGGTGGCCCTAGTCCGCTCTTCTCAAGCTCTACATCAATTTGGAAGAAGAACTTACATGTAACGAGTTCATTATTACTAGGGTCATCGGATCTAATAATACAATATTTAGAGAGAAACTTACACATAACATTGAATTATTATATTAAAACGCCACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

182

Amino Acids

20.19

Weight (kDa)

8.91

Isoelectric Point (pI)

45.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 289
AccB7I CCANNNNNTGG 1 cut(s) 36
AccBSI CCGCTC 1 cut(s) 403
AccII CGCG 1 cut(s) 93
AciI CCGC 1 cut(s) 401
AclWI GGATC 1 cut(s) 483
AfiI CCNNNNNNNGG 2 cut(s) 36, 389
AflIII ACRYGT 1 cut(s) 442
AgsI TTSAA 2 cut(s) 374, 520
AluBI AGCT 3 cut(s) 313, 346, 414
AluI AGCT 3 cut(s) 313, 346, 414
Alw26I GTCTC 1 cut(s) 332
AlwI GGATC 1 cut(s) 483
AoxI GGCC 2 cut(s) 362, 391
ApeKI GCWGC 2 cut(s) 212, 346
AspS9I GGNCC 1 cut(s) 392
AsuHPI GGTGA 2 cut(s) 92, 235
AxyI CCTNAGG 1 cut(s) 384
BbvI GCAGC 2 cut(s) 224, 333
BccI CCATC 3 cut(s) 62, 148, 323
BcoDI GTCTC 1 cut(s) 332
BfaI CTAG 3 cut(s) 62, 396, 464
BfuAI ACCTGC 1 cut(s) 289
BisI GCNGC 2 cut(s) 213, 347
BlpI GCTNAGC 1 cut(s) 309
BlsI GCNGC 2 cut(s) 214, 348
BmgT120I GGNCC 1 cut(s) 392
BplI GAGNNNNNCTC 2 cut(s) 352, 384
Bpu1102I GCTNAGC 1 cut(s) 309
BpuEI CTTGAG 1 cut(s) 394
BsaBI GATNNNNATC 1 cut(s) 233
BsaI GGTCTC 1 cut(s) 332
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bsc4I CCNNNNNNNGG 2 cut(s) 36, 389
Bse21I CCTNAGG 1 cut(s) 384
Bse8I GATNNNNATC 1 cut(s) 233
BseJI GATNNNNATC 1 cut(s) 233
BseLI CCNNNNNNNGG 2 cut(s) 36, 389
BseMII CTCAG 2 cut(s) 323, 375
BseRI GAGGAG 1 cut(s) 269
BseXI GCAGC 2 cut(s) 224, 333
BseYI CCCAGC 1 cut(s) 342
BsgI GTGCAG 1 cut(s) 231
Bsh1236I CGCG 1 cut(s) 93
BshFI GGCC 2 cut(s) 364, 393
BslI CCNNNNNNNGG 2 cut(s) 36, 389
BsmAI GTCTC 1 cut(s) 332
BsmI GAATGC 1 cut(s) 378
BsnI GGCC 2 cut(s) 364, 393
Bso31I GGTCTC 1 cut(s) 332
Bsp143I GATC 5 cut(s) 94, 111, 228, 234, 475
Bsp1720I GCTNAGC 1 cut(s) 309
Bsp68I TCGCGA 1 cut(s) 93
BspACI CCGC 1 cut(s) 401
BspANI GGCC 2 cut(s) 364, 393
BspCNI CTCAG 2 cut(s) 322, 376
BspFNI CGCG 1 cut(s) 93
BspHI TCATGA 1 cut(s) 114
BspMI ACCTGC 1 cut(s) 289
BspPI GGATC 1 cut(s) 483
BspQI GCTCTTC 2 cut(s) 375, 410
BspTNI GGTCTC 1 cut(s) 332
BsrBI CCGCTC 1 cut(s) 403
BssMI GATC 5 cut(s) 94, 111, 228, 234, 475
Bst6I CTCTTC 2 cut(s) 375, 410
BstC8I GCNNGC 2 cut(s) 148, 366
BstDEI CTNAG 2 cut(s) 309, 384
BstFNI CGCG 1 cut(s) 93
BstKTI GATC 5 cut(s) 97, 114, 231, 237, 478
BstMAI GTCTC 1 cut(s) 332
BstMBI GATC 5 cut(s) 94, 111, 228, 234, 475
BstNSI RCATGY 1 cut(s) 446
BstUI CGCG 1 cut(s) 93
BstV1I GCAGC 2 cut(s) 224, 333
BstX2I RGATCY 1 cut(s) 475
BstYI RGATCY 1 cut(s) 475
Bsu36I CCTNAGG 1 cut(s) 384
BsuRI GGCC 2 cut(s) 364, 393
BtgZI GCGATG 1 cut(s) 342
BtuMI TCGCGA 1 cut(s) 93
BveI ACCTGC 1 cut(s) 289
Cac8I GCNNGC 2 cut(s) 148, 366
CciI TCATGA 1 cut(s) 114
Cfr13I GGNCC 1 cut(s) 392
CviAII CATG 4 cut(s) 20, 31, 115, 443
CviJI RGCY 8 cut(s) 150, 308, 313, 333, 346, 364, 393, 414
CviKI_1 RGCY 8 cut(s) 150, 308, 313, 333, 346, 364, 393, 414
DdeI CTNAG 2 cut(s) 309, 384
DpnI GATC 5 cut(s) 96, 113, 230, 236, 477
DpnII GATC 5 cut(s) 94, 111, 228, 234, 475
Eam1104I CTCTTC 2 cut(s) 375, 410
EarI CTCTTC 2 cut(s) 375, 410
Eco147I AGGCCT 1 cut(s) 364
Eco31I GGTCTC 1 cut(s) 332
Eco81I CCTNAGG 1 cut(s) 384
FaeI CATG 4 cut(s) 23, 34, 118, 446
FaiI YATR 8 cut(s) 14, 21, 32, 116, 279, 444, 512, 528
FalI AAGNNNNNCTT 2 cut(s) 422, 454
FatI CATG 4 cut(s) 19, 30, 114, 442
Fnu4HI GCNGC 2 cut(s) 213, 347
Fsp4HI GCNGC 2 cut(s) 213, 347
FspBI CTAG 3 cut(s) 62, 396, 464
GluI GCNGC 2 cut(s) 213, 347
GsaI CCCAGC 1 cut(s) 346
HaeIII GGCC 2 cut(s) 364, 393
Hin1II CATG 4 cut(s) 23, 34, 118, 446
HinfI GANTC 2 cut(s) 23, 158
HphI GGTGA 2 cut(s) 92, 235
Hpy188I TCNGA 1 cut(s) 475
Hpy188III TCNNGA 4 cut(s) 92, 115, 232, 383
HpyAV CCTTC 1 cut(s) 70
HpyCH4IV ACGT 1 cut(s) 105
HpyCH4V TGCA 4 cut(s) 12, 140, 212, 349
HpyF3I CTNAG 2 cut(s) 309, 384
HpySE526I ACGT 1 cut(s) 105
Hsp92II CATG 4 cut(s) 23, 34, 118, 446
Kzo9I GATC 5 cut(s) 94, 111, 228, 234, 475
LguI GCTCTTC 2 cut(s) 375, 410
LpnPI CCDG 6 cut(s) 27, 164, 284, 356, 378, 396
Lsp1109I GCAGC 2 cut(s) 224, 333
MaeI CTAG 3 cut(s) 62, 396, 464
MaeII ACGT 1 cut(s) 105
MaeIII GTNAC 2 cut(s) 80, 445
MalI GATC 5 cut(s) 96, 113, 230, 236, 477
MbiI CCGCTC 1 cut(s) 403
MboI GATC 5 cut(s) 94, 111, 228, 234, 475
MboII GAAGA 8 cut(s) 130, 133, 145, 238, 362, 397, 442, 445
MflI RGATCY 1 cut(s) 475
MluCI AATT 3 cut(s) 7, 423, 520
MmeI TCCRAC 1 cut(s) 231
MnlI CCTC 5 cut(s) 40, 214, 247, 354, 379
MseI TTAA 1 cut(s) 531
MspA1I CMGCKG 2 cut(s) 313, 346
Mva1269I GAATGC 1 cut(s) 378
MvnI CGCG 1 cut(s) 93
NdeII GATC 5 cut(s) 94, 111, 228, 234, 475
NlaIII CATG 4 cut(s) 23, 34, 118, 446
NmuCI GTSAC 1 cut(s) 80
NruI TCGCGA 1 cut(s) 93
NspI RCATGY 1 cut(s) 446
PagI TCATGA 1 cut(s) 114
PceI AGGCCT 1 cut(s) 364
PciI ACATGT 1 cut(s) 442
PciSI GCTCTTC 2 cut(s) 375, 410
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PctI GAATGC 1 cut(s) 378
PfeI GAWTC 2 cut(s) 23, 158
PflMI CCANNNNNTGG 1 cut(s) 36
PkrI GCNGC 2 cut(s) 214, 348
PscI ACATGT 1 cut(s) 442
PspFI CCCAGC 1 cut(s) 342
PspPI GGNCC 1 cut(s) 392
PsuI RGATCY 1 cut(s) 475
PvuII CAGCTG 2 cut(s) 313, 346
RruI TCGCGA 1 cut(s) 93
SapI GCTCTTC 2 cut(s) 375, 410
SaqAI TTAA 1 cut(s) 531
SatI GCNGC 2 cut(s) 213, 347
Sau3AI GATC 5 cut(s) 94, 111, 228, 234, 475
Sau96I GGNCC 1 cut(s) 392
SetI ASST 8 cut(s) 108, 225, 273, 303, 315, 348, 356, 416
SmlI CTYRAG 1 cut(s) 409
SmoI CTYRAG 1 cut(s) 409
Sse9I AATT 3 cut(s) 7, 423, 520
SseBI AGGCCT 1 cut(s) 364
SsiI CCGC 1 cut(s) 401
SspI AATATT 1 cut(s) 491
SspMI CTAG 3 cut(s) 62, 396, 464
StuI AGGCCT 1 cut(s) 364
TaiI ACGT 1 cut(s) 108
TasI AATT 3 cut(s) 7, 423, 520
TfiI GAWTC 2 cut(s) 23, 158
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TseFI GTSAC 1 cut(s) 80
TseI GCWGC 2 cut(s) 212, 346
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 5 cut(s) 47, 89, 131, 150, 444
TspGWI ACGGA 1 cut(s) 116
Van91I CCANNNNNTGG 1 cut(s) 36
XceI RCATGY 1 cut(s) 446
XspI CTAG 3 cut(s) 62, 396, 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.