Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0012029

Physical Location & Seq

Genomic Coordinates Reverse (-)

23463023 .. 23466508

Gene Structure 3486 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G63250.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2397 bp

ATGTACTCGTTAATTCTCCGGGAAAGATCTGGATCCATCACAGGGTCCTTATGGAACCGAATATCCTCTCGGAACATGGGTGGTGGTCCGAGAACCTTCCCTGGAGGACTAAACAAGTGGCAGTGGAAACGTATGCACGAGAAGAAAGCTAGAGAGAAAGAGAACAAGCTCTTGGATCAAGAGAAACAGCTTTACGAAGCTCGAATTAGGACCGAGATCCGAGCTAAAATGTGGGGAAATCCAGATTCTGGTGAGAAAACGGCGAAATCGAAGCAGAGTCATGGACCAATGAGCCCGAAAGAACATATCAAAACCTTGGCTGATCGGTTTATGAAAGCTGGAGCAGAGGATTTTTGGAACGAGAACGATGGTCCGGTGAAGAAATCAGATCAAGGGTCGAGATCAGGTTCGGATTCGATTGATTCTACTTCTAATTCGCCTATTGATGTGAGGAGATTAGTTTCTGCGACATGTGATTCTATGGGTAAAAATCGTGTTTTCGGTAGTAGTAGGAGAGGTTTTTCGAGTATGAGTAGATTTAAGAGAAATGAGAGTTCTTGTGATGAAGGAGATGACTTTGATGCTAAAAAGCTAGATACTTTGAGTCCTTTTTCTCCTAAATTTGCTGGTACCAAGGAGAAAGTGAAGAGCTCGAGGAGTGTTGTTGGAGTTATAAGGAACAAAGGTTTGTTTGGAAGAAGGAAGTTTAGGAAGAACGATAGTTCAACTGAAGAGGATTCAGATGAAGAAGGCGATGAGGGAAAGATGATAGGTTGGATGGATATGAGGAAAACGGGAAGCAGTGCGTCGTTAGGGAACCACGATATCAAGCTGACGAAAAGAGTGAATCGTAATGTCACGGATGAAGAGCTTTATCCTCCTTTGGATATTAATACAGTTAGAGAAGATCTCAGTAAAAGAAAATCTGTGGATAATGTAATGGAGGAGAAGCAAGAGCCTCATGACTCGATTTACAGTGCAAAAAGATTTGATGAGTCTTGTATATCTCCTTTGACGCTAAAGGCACTTTCTGCGTCTGGCATTCTTAAGATGACTAGAGTACAGGATGCCACTCTCTCTGAGTGCCTAGACGGTAAAGATGCATTAGTCAAAGCCAAAACTGGGACGGGAAAGAGCATGGCGTTTTTGCTTCCTGCAATTGAAACAGTTCTGAAAGCTATGAACAGCGGTAAGGGCGTTAACAAAGTTGCTCCGATTTTTGCTCTTATCCTCTGTCCCACAAGAGAACTTGCAAGCCAGATCGCTGCAGAGGGCAAGGCTCTACTCAAATTCCATGACGGGATTGGTGTTCAGACTCTAATTGGTGGTACACGCTTCAAACTTGATCAACAACGCCTAGAATCTGAGCCATGTCAGATACTAATAGCAACTCCGGGAAGACTCTTGGATCATATAGAGAATAAGTCTGGCTTAACATCGCGTTTGATGGCGCTTAAATTGTTTATAGTTGACGAGGCTGACCTTTTGCTAGACTTAGGATTCAGGAGAGATGTCGAAAAAATCATAGATTGTTTGCCTCGTCAGAGACAATCTCTTCTCTTTTCTGCAACCATTCCGAAAGAGGTCCGTCGAGTCTCACAGCTTGTTTTGAAAAGGGATCACTCTTATATCGACACAATCGGGCTCGGTTGTGTAGAGACTCATGATAAGGTGAGGCAATCTTGTATTGTTGCTCCACACGAATCTCATTTCCACTTAGTACCTCATCTTCTAAAAGAGCACATCAACAACACGCCTGATTATAAGATAATAGTCTTTTGCTCAACCGGTATGGTAACATCACTGATGTATACTCTACTCCGGGAAATGAAATTGAACGTTAGGGAGATTCATGCTAGAAAACCTCAGCTCCATAGAACACGTGTATCAGACGAGTTTAAGGAATCAAATAGACTGATTCTTGTCACATCTGATGTATCTGCTCGTGGAATGAACTATCCAGATGTTACTTTGGTTATTCAGGTCGGTATACCGAGTGACAGAGAACAGTATATACATCGACTAGGTAGAACTGGAAGAGAAGGAAAAGGAGGAGAAGGATTGCTTTTGATTGCGCCATGGGAGAGATATTTTCTTGATGAGCTTAAAGATTTGCCACTTGAACCAATTCCAGCTCCGGATCTTGATTCAATAGTTAAACATCAGGTTGATCAGTCGATGGCTAAGATTGATACAAGTATCAAAGAAGCAGCGTACCATGCATGGCTCGGTTATTACAACTCGGTTCGAGAAACCGGTAGAGACAAAACCACGTTAGCCGAGTTAGCTAATCGGTTTTGCCATTCTATCGGTTTAGAGAAACCTCCTGCTTTGTTTCGAAGAACCGCGGTTAAGATGGGTCTCAAAGGTATTTCTGGTATTCCAATCCGAAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

798

Amino Acids

89.59

Weight (kDa)

9.36

Isoelectric Point (pI)

46.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	352 - 531	4.8e-43	DEAD/DEAH box helicase
Helicase_C	PF00271	577 - 682	2.6e-22	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012029)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63250 AT2G07750 AT5G08620
fragaria_vesca	FvH4_5g01920
malus_domestica	MD06G1077400.v1.1
prunus_persica	Prupe.5G095400_v2.0.a1 Prupe.5G095400_v2.0.a1 Prupe.5G095400_v2.0.a1
pyrus_communis	pycom06g07520
rosa_chinensis	RchiOBHm_Chr7g0198601
rosa_laevigata	RLG00000003858
rosa_multiflora	Rmu_sc0004229.1_g000005
rosa_roxburghii	Rroxscaffold_3G00257630
rosa_rugosa	Rorug07G0048400
rosa_samantha	Rh7AG171900 Rh7BG175300 Rh7CG181400 Rh7DG174400
rosa_wichuraiana	Rw7G015080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	674, 1764
Acc65I	GGTACC	1 cut(s)	629
AccB1I	GGYRCC	1 cut(s)	629
AccB7I	CCANNNNNTGG	1 cut(s)	248
AccI	GTMKAC	2 cut(s)	1811, 1990
AccII	CGCG	2 cut(s)	1441, 2348
AccIII	TCCGGA	1 cut(s)	2137
AciI	CCGC	3 cut(s)	1188, 2346, 2348
AclI	AACGTT	1 cut(s)	1839
AclWI	GGATC	7 cut(s)	27, 40, 183, 211, 1416, 1626, 2148
AcsI	RAATTY	2 cut(s)	620, 1289
AcuI	CTGAAG	1 cut(s)	750
AcvI	CACGTG	1 cut(s)	1883
AfaI	GTAC	6 cut(s)	5, 631, 1062, 1330, 1722, 2216
AfiI	CCNNNNNNNGG	3 cut(s)	42, 248, 1122
AflII	CTTAAG	1 cut(s)	1046
AflIII	ACRYGT	3 cut(s)	470, 1880, 1882
AgeI	ACCGGT	2 cut(s)	1787, 2255
AgsI	TTSAA	7 cut(s)	726, 1163, 1339, 1612, 1837, 2123, 2151
AjnI	CCWGG	1 cut(s)	100
AjuI	GAANNNNNNNTTGG	2 cut(s)	155, 187
AloI	GAACNNNNNNTCC	2 cut(s)	47, 79
Alw21I	GWGCWC	2 cut(s)	653, 1743
Alw26I	GTCTC	5 cut(s)	1540, 1600, 1652, 2256, 2366
AlwI	GGATC	7 cut(s)	27, 40, 183, 211, 1416, 1626, 2148
AlwNI	CAGNNNCTG	1 cut(s)	248
Ama87I	CYCGRG	1 cut(s)	652
Aor13HI	TCCGGA	1 cut(s)	2137
ApeKI	GCWGC	2 cut(s)	1265, 2210
ApoI	RAATTY	2 cut(s)	620, 1289
AseI	ATTAAT	1 cut(s)	891
AsiGI	ACCGGT	2 cut(s)	1787, 2255
Asp700I	GAANNNNTTC	2 cut(s)	1167, 2127
Asp718I	GGTACC	1 cut(s)	629
AspLEI	GCGC	2 cut(s)	1453, 2077
AspS9I	GGNCC	6 cut(s)	45, 86, 210, 284, 371, 1585
AsuC2I	CCSGG	3 cut(s)	20, 1395, 1823
AsuHPI	GGTGA	3 cut(s)	263, 388, 1684
AsuII	TTCGAA	1 cut(s)	2338
AvaI	CYCGRG	1 cut(s)	652
AvaII	GGWCC	6 cut(s)	45, 86, 210, 284, 371, 1585
BaeI	ACNNNNGTAYC	2 cut(s)	1869, 1902
BamHI	GGATCC	1 cut(s)	32
BanI	GGYRCC	1 cut(s)	629
BanII	GRGCYC	3 cut(s)	296, 653, 1647
BarI	GAAGNNNNNNTAC	2 cut(s)	1713, 1745
BauI	CACGAG	2 cut(s)	137, 1944
BbrPI	CACGTG	1 cut(s)	1883
BbsI	GAAGAC	1 cut(s)	1405
Bbv12I	GWGCWC	2 cut(s)	653, 1743
BbvCI	CCTCAGC	1 cut(s)	1866
BbvI	GCAGC	2 cut(s)	1252, 2222
BccI	CCATC	6 cut(s)	44, 362, 772, 1441, 2173, 2350
BceAI	ACGGC	1 cut(s)	276
BciT130I	CCWGG	1 cut(s)	102
BclI	TGATCA	2 cut(s)	1345, 2170
BcnI	CCSGG	3 cut(s)	20, 1395, 1823
BcoDI	GTCTC	5 cut(s)	1540, 1600, 1652, 2256, 2366
BfaI	CTAG	8 cut(s)	150, 593, 1056, 1088, 1358, 1490, 1857, 2024
BfmI	CTRYAG	1 cut(s)	1266
BfoI	RGCGCY	1 cut(s)	1454
BfrI	CTTAAG	1 cut(s)	1046
BglII	AGATCT	2 cut(s)	26, 907
BisI	GCNGC	2 cut(s)	1266, 2211
BlsI	GCNGC	2 cut(s)	1267, 2212
Bme1390I	CCNGG	4 cut(s)	20, 102, 1395, 1823
Bme18I	GGWCC	6 cut(s)	45, 86, 210, 284, 371, 1585
BmeT110I	CYCGRG	1 cut(s)	652
BmgT120I	GGNCC	6 cut(s)	45, 86, 210, 284, 371, 1585
BmiI	GGNNCC	5 cut(s)	34, 46, 56, 631, 818
BmrFI	CCNGG	4 cut(s)	20, 102, 1395, 1823
BmrI	ACTGGG	1 cut(s)	1131
BmsI	GCATC	3 cut(s)	571, 1057, 1090
BmuI	ACTGGG	1 cut(s)	1131
BpiI	GAAGAC	1 cut(s)	1405
BplI	GAGNNNNNCTC	4 cut(s)	894, 926, 1537, 1569
BpmI	CTGGAG	2 cut(s)	123, 360
Bpu10I	CCTNAGC	1 cut(s)	1866
Bpu14I	TTCGAA	1 cut(s)	2338
BpuMI	CCSGG	3 cut(s)	20, 1395, 1823
BsaAI	YACGTR	1 cut(s)	1883
BsaBI	GATNNNNATC	1 cut(s)	31
BsaI	GGTCTC	1 cut(s)	2366
BsaJI	CCNNGG	5 cut(s)	100, 315, 633, 2078, 2346
BsaWI	WCCGGW	4 cut(s)	373, 1787, 2137, 2255
BsaXI	ACNNNNNCTCC	2 cut(s)	1854, 1884
Bsc4I	CCNNNNNNNGG	3 cut(s)	42, 248, 1122
Bse118I	RCCGGY	2 cut(s)	1787, 2255
Bse1I	ACTGG	2 cut(s)	1126, 2038
Bse8I	GATNNNNATC	1 cut(s)	31
BseAI	TCCGGA	1 cut(s)	2137
BseBI	CCWGG	1 cut(s)	102
BseDI	CCNNGG	5 cut(s)	100, 315, 633, 2078, 2346
BseGI	GGATG	3 cut(s)	783, 868, 1072
BseJI	GATNNNNATC	1 cut(s)	31
BseLI	CCNNNNNNNGG	3 cut(s)	42, 248, 1122
BseMII	CTCAG	4 cut(s)	925, 1071, 1356, 1880
BseNI	ACTGG	2 cut(s)	1126, 2038
BseRI	GAGGAG	4 cut(s)	466, 670, 959, 2067
BseXI	GCAGC	2 cut(s)	1252, 2222
Bsh1236I	CGCG	2 cut(s)	1441, 2348
BshNI	GGYRCC	1 cut(s)	629
BshTI	ACCGGT	2 cut(s)	1787, 2255
BsiHKAI	GWGCWC	2 cut(s)	653, 1743
BsiHKCI	CYCGRG	1 cut(s)	652
BsiSI	CCGG	7 cut(s)	19, 374, 1394, 1788, 1822, 2138, 2256
BslFI	GGGAC	2 cut(s)	1138, 1221
BslI	CCNNNNNNNGG	3 cut(s)	42, 248, 1122
BsmAI	GTCTC	5 cut(s)	1540, 1600, 1652, 2256, 2366
BsmFI	GGGAC	2 cut(s)	1138, 1221
BsmI	GAATGC	1 cut(s)	1041
Bso31I	GGTCTC	1 cut(s)	2366
BsoBI	CYCGRG	1 cut(s)	652
Bsp119I	TTCGAA	1 cut(s)	2338
Bsp1286I	GDGCHC	4 cut(s)	296, 653, 1647, 1743
Bsp13I	TCCGGA	1 cut(s)	2137
Bsp19I	CCATGG	1 cut(s)	2078
BspACI	CCGC	3 cut(s)	1188, 2346, 2348
BspCNI	CTCAG	4 cut(s)	924, 1072, 1357, 1879
BspEI	TCCGGA	1 cut(s)	2137
BspFNI	CGCG	2 cut(s)	1441, 2348
BspHI	TCATGA	2 cut(s)	961, 1663
BspLI	GGNNCC	5 cut(s)	34, 46, 56, 631, 818
BspMAI	CTGCAG	1 cut(s)	1270
BspPI	GGATC	7 cut(s)	27, 40, 183, 211, 1416, 1626, 2148
BspQI	GCTCTTC	2 cut(s)	641, 861
BspT104I	TTCGAA	1 cut(s)	2338
BspT107I	GGYRCC	1 cut(s)	629
BspTI	CTTAAG	1 cut(s)	1046
BspTNI	GGTCTC	1 cut(s)	2366
BsrFI	RCCGGY	2 cut(s)	1787, 2255
BsrI	ACTGG	2 cut(s)	1126, 2038
BssAI	RCCGGY	2 cut(s)	1787, 2255
BssECI	CCNNGG	5 cut(s)	100, 315, 633, 2078, 2346
BssNAI	GTATAC	2 cut(s)	1812, 1991
BssSI	CACGAG	2 cut(s)	137, 1944
BssT1I	CCWWGG	3 cut(s)	315, 633, 2078
Bst1107I	GTATAC	2 cut(s)	1812, 1991
Bst2BI	CACGAG	2 cut(s)	137, 1944
Bst2UI	CCWGG	1 cut(s)	102
Bst4CI	ACNGT	5 cut(s)	898, 977, 1094, 1168, 2010
Bst6I	CTCTTC	5 cut(s)	641, 726, 861, 1560, 2032
BstAFI	CTTAAG	1 cut(s)	1046
BstAPI	GCANNNNNTGC	1 cut(s)	1031
BstBAI	YACGTR	1 cut(s)	1883
BstBI	TTCGAA	1 cut(s)	2338
BstC8I	GCNNGC	1 cut(s)	1255
BstDEI	CTNAG	7 cut(s)	911, 1080, 1365, 1495, 1717, 1866, 2184
BstDSI	CCRYGG	2 cut(s)	2078, 2346
BstF5I	GGATG	3 cut(s)	783, 868, 1072
BstFNI	CGCG	2 cut(s)	1441, 2348
BstH2I	RGCGCY	1 cut(s)	1454
BstHHI	GCGC	2 cut(s)	1453, 2077
BstMAI	GTCTC	5 cut(s)	1540, 1600, 1652, 2256, 2366
BstMWI	GCNNNNNNNGC	4 cut(s)	1031, 1194, 2219, 2285
BstNI	CCWGG	1 cut(s)	102
BstNSI	RCATGY	1 cut(s)	474
BstSCI	CCNGG	4 cut(s)	18, 100, 1393, 1821
BstSFI	CTRYAG	1 cut(s)	1266
BstUI	CGCG	2 cut(s)	1441, 2348
BstV1I	GCAGC	2 cut(s)	1252, 2222
BstV2I	GAAGAC	1 cut(s)	1405
BstX2I	RGATCY	5 cut(s)	26, 32, 216, 907, 2140
BstYI	RGATCY	5 cut(s)	26, 32, 216, 907, 2140
BstZ17I	GTATAC	2 cut(s)	1812, 1991
BtgI	CCRYGG	2 cut(s)	2078, 2346
BtgZI	GCGATG	2 cut(s)	768, 1422
BtsCI	GGATG	3 cut(s)	783, 868, 1072
BtsI	GCAGTG	2 cut(s)	128, 808
BtsIMutI	CAGTG	4 cut(s)	128, 808, 982, 1802
Cac8I	GCNNGC	1 cut(s)	1255
CaiI	CAGNNNCTG	1 cut(s)	248
CciI	TCATGA	2 cut(s)	961, 1663
CfoI	GCGC	2 cut(s)	1453, 2077
Cfr10I	RCCGGY	2 cut(s)	1787, 2255
Cfr13I	GGNCC	6 cut(s)	45, 86, 210, 284, 371, 1585
Cfr42I	CCGCGG	1 cut(s)	2349
CseI	GACGC	3 cut(s)	795, 1023, 1024
Csp6I	GTAC	6 cut(s)	4, 630, 1061, 1329, 1721, 2215
CspAI	ACCGGT	2 cut(s)	1787, 2255
CviQI	GTAC	6 cut(s)	4, 630, 1061, 1329, 1721, 2215
DdeI	CTNAG	7 cut(s)	911, 1080, 1365, 1495, 1717, 1866, 2184
Eam1104I	CTCTTC	5 cut(s)	641, 726, 861, 1560, 2032
EarI	CTCTTC	5 cut(s)	641, 726, 861, 1560, 2032
Ecl136II	GAGCTC	1 cut(s)	651
Eco130I	CCWWGG	3 cut(s)	315, 633, 2078
Eco24I	GRGCYC	3 cut(s)	296, 653, 1647
Eco31I	GGTCTC	1 cut(s)	2366
Eco32I	GATATC	1 cut(s)	826
Eco47I	GGWCC	6 cut(s)	45, 86, 210, 284, 371, 1585
Eco53kI	GAGCTC	1 cut(s)	651
Eco57I	CTGAAG	1 cut(s)	750
Eco72I	CACGTG	1 cut(s)	1883
Eco88I	CYCGRG	1 cut(s)	652
EcoICRI	GAGCTC	1 cut(s)	651
EcoO109I	RGGNCCY	1 cut(s)	45
EcoRII	CCWGG	1 cut(s)	100
EcoRV	GATATC	1 cut(s)	826
EcoT14I	CCWWGG	3 cut(s)	315, 633, 2078
EcoT22I	ATGCAT	2 cut(s)	1105, 2224
EcoT38I	GRGCYC	3 cut(s)	296, 653, 1647
ErhI	CCWWGG	3 cut(s)	315, 633, 2078
FalI	AAGNNNNNCTT	4 cut(s)	1415, 1447, 2049, 2081
FaqI	GGGAC	2 cut(s)	1138, 1221
FbaI	TGATCA	2 cut(s)	1345, 2170
FblI	GTMKAC	2 cut(s)	1811, 1990
Fnu4HI	GCNGC	2 cut(s)	1266, 2211
FokI	GGATG	3 cut(s)	790, 875, 1079
FriOI	GRGCYC	3 cut(s)	296, 653, 1647
Fsp4HI	GCNGC	2 cut(s)	1266, 2211
FspBI	CTAG	8 cut(s)	150, 593, 1056, 1088, 1358, 1490, 1857, 2024
GlaI	GCGC	2 cut(s)	1452, 2076
GluI	GCNGC	2 cut(s)	1266, 2211
GsuI	CTGGAG	2 cut(s)	123, 360
HaeII	RGCGCY	1 cut(s)	1454
HapII	CCGG	7 cut(s)	19, 374, 1394, 1788, 1822, 2138, 2256
HgaI	GACGC	3 cut(s)	795, 1023, 1024
HhaI	GCGC	2 cut(s)	1453, 2077
Hin6I	GCGC	2 cut(s)	1451, 2075
HinP1I	GCGC	2 cut(s)	1451, 2075
HincII	GTYRAC	2 cut(s)	1201, 1471
HindII	GTYRAC	2 cut(s)	1201, 1471
HpaI	GTTAAC	1 cut(s)	1201
HpaII	CCGG	7 cut(s)	19, 374, 1394, 1788, 1822, 2138, 2256
HphI	GGTGA	3 cut(s)	263, 388, 1684
Hpy166II	GTNNAC	5 cut(s)	1201, 1331, 1471, 1812, 1991
Hpy8I	GTNNAC	5 cut(s)	1201, 1331, 1471, 1812, 1991
Hpy99I	CGWCG	2 cut(s)	811, 1593
HpyAV	CCTTC	6 cut(s)	106, 560, 693, 743, 2036, 2051
HpyCH4III	ACNGT	5 cut(s)	898, 977, 1094, 1168, 2010
HpyCH4IV	ACGT	4 cut(s)	130, 1839, 1882, 2273
HpyCH4V	TGCA	8 cut(s)	136, 980, 1103, 1157, 1253, 1268, 1568, 2222
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1031, 1194, 2219, 2285
HpyF3I	CTNAG	7 cut(s)	911, 1080, 1365, 1495, 1717, 1866, 2184
HpySE526I	ACGT	4 cut(s)	130, 1839, 1882, 2273
HspAI	GCGC	2 cut(s)	1451, 2075
Kpn2I	TCCGGA	1 cut(s)	2137
KpnI	GGTACC	1 cut(s)	633
Ksp22I	TGATCA	2 cut(s)	1345, 2170
KspAI	GTTAAC	1 cut(s)	1201
KspI	CCGCGG	1 cut(s)	2349
LguI	GCTCTTC	2 cut(s)	641, 861
LmnI	GCTCC	5 cut(s)	341, 1216, 1699, 1875, 2140
Lsp1109I	GCAGC	2 cut(s)	1252, 2222
LweI	GCATC	3 cut(s)	571, 1057, 1090
MaeI	CTAG	8 cut(s)	150, 593, 1056, 1088, 1358, 1490, 1857, 2024
MaeII	ACGT	4 cut(s)	130, 1839, 1882, 2273
MaeIII	GTNAC	5 cut(s)	856, 1795, 1924, 1966, 1997
MfeI	CAATTG	1 cut(s)	1158
MflI	RGATCY	5 cut(s)	26, 32, 216, 907, 2140
MhlI	GDGCHC	4 cut(s)	296, 653, 1647, 1743
MlyI	GAGTC	8 cut(s)	286, 613, 959, 1004, 1309, 1395, 1602, 1654
MmeI	TCCRAC	2 cut(s)	646, 755
Mph1103I	ATGCAT	2 cut(s)	1105, 2224
MroI	TCCGGA	1 cut(s)	2137
MroXI	GAANNNNTTC	2 cut(s)	1167, 2127
MspA1I	CMGCKG	2 cut(s)	1188, 2348
MspCI	CTTAAG	1 cut(s)	1046
MspI	CCGG	7 cut(s)	19, 374, 1394, 1788, 1822, 2138, 2256
MspR9I	CCNGG	4 cut(s)	20, 102, 1395, 1823
MunI	CAATTG	1 cut(s)	1158
Mva1269I	GAATGC	1 cut(s)	1041
MvaI	CCWGG	1 cut(s)	102
MvnI	CGCG	2 cut(s)	1441, 2348
MwoI	GCNNNNNNNGC	4 cut(s)	1031, 1194, 2219, 2285
NciI	CCSGG	3 cut(s)	20, 1395, 1823
NcoI	CCATGG	1 cut(s)	2078
NlaIV	GGNNCC	5 cut(s)	34, 46, 56, 631, 818
NmeAIII	GCCGAG	1 cut(s)	2305
NmuCI	GTSAC	3 cut(s)	856, 1924, 1997
NsiI	ATGCAT	2 cut(s)	1105, 2224
NspI	RCATGY	1 cut(s)	474
NspV	TTCGAA	1 cut(s)	2338
PaeR7I	CTCGAG	1 cut(s)	652
PagI	TCATGA	2 cut(s)	961, 1663
PciI	ACATGT	1 cut(s)	470
PciSI	GCTCTTC	2 cut(s)	641, 861
PcsI	WCGNNNNNNNCGW	1 cut(s)	266
PctI	GAATGC	1 cut(s)	1041
PdmI	GAANNNNTTC	2 cut(s)	1167, 2127
PflMI	CCANNNNNTGG	1 cut(s)	248
PfoI	TCCNGGA	3 cut(s)	18, 1393, 1821
PinAI	ACCGGT	2 cut(s)	1787, 2255
PkrI	GCNGC	2 cut(s)	1267, 2212
PleI	GAGTC	8 cut(s)	285, 612, 959, 1003, 1309, 1395, 1601, 1654
PmaCI	CACGTG	1 cut(s)	1883
PmlI	CACGTG	1 cut(s)	1883
PpsI	GAGTC	8 cut(s)	285, 612, 959, 1003, 1309, 1395, 1601, 1654
Ppu21I	YACGTR	1 cut(s)	1883
PpuMI	RGGWCCY	1 cut(s)	45
PscI	ACATGT	1 cut(s)	470
PshBI	ATTAAT	1 cut(s)	891
PsiI	TTATAA	2 cut(s)	674, 1764
Psp124BI	GAGCTC	1 cut(s)	653
Psp1406I	AACGTT	1 cut(s)	1839
Psp5II	RGGWCCY	1 cut(s)	45
Psp6I	CCWGG	1 cut(s)	100
PspCI	CACGTG	1 cut(s)	1883
PspGI	CCWGG	1 cut(s)	100
PspN4I	GGNNCC	5 cut(s)	34, 46, 56, 631, 818
PspPI	GGNCC	6 cut(s)	45, 86, 210, 284, 371, 1585
PspPPI	RGGWCCY	1 cut(s)	45
PspXI	VCTCGAGB	1 cut(s)	652
PsrI	GAACNNNNNNTAC	2 cut(s)	1998, 2030
PstI	CTGCAG	1 cut(s)	1270
PstNI	CAGNNNCTG	1 cut(s)	248
PsuI	RGATCY	5 cut(s)	26, 32, 216, 907, 2140
RsaI	GTAC	6 cut(s)	5, 631, 1062, 1330, 1722, 2216
RsaNI	GTAC	6 cut(s)	4, 630, 1061, 1329, 1721, 2215
SacI	GAGCTC	1 cut(s)	653
SacII	CCGCGG	1 cut(s)	2349
SapI	GCTCTTC	2 cut(s)	641, 861
SatI	GCNGC	2 cut(s)	1266, 2211
Sau96I	GGNCC	6 cut(s)	45, 86, 210, 284, 371, 1585
SchI	GAGTC	8 cut(s)	286, 613, 959, 1004, 1309, 1395, 1602, 1654
ScrFI	CCNGG	4 cut(s)	20, 102, 1395, 1823
SduI	GDGCHC	4 cut(s)	296, 653, 1647, 1743
SfaNI	GCATC	3 cut(s)	571, 1057, 1090
SfcI	CTRYAG	1 cut(s)	1266
Sfr274I	CTCGAG	1 cut(s)	652
Sfr303I	CCGCGG	1 cut(s)	2349
SfuI	TTCGAA	1 cut(s)	2338
SgrBI	CCGCGG	1 cut(s)	2349
SinI	GGWCC	6 cut(s)	45, 86, 210, 284, 371, 1585
SlaI	CTCGAG	1 cut(s)	652
SmlI	CTYRAG	2 cut(s)	652, 1046
SmoI	CTYRAG	2 cut(s)	652, 1046
SsiI	CCGC	3 cut(s)	1188, 2346, 2348
SspMI	CTAG	8 cut(s)	150, 593, 1056, 1088, 1358, 1490, 1857, 2024
SstI	GAGCTC	1 cut(s)	653
StyD4I	CCNGG	4 cut(s)	18, 100, 1393, 1821
StyI	CCWWGG	3 cut(s)	315, 633, 2078
TaaI	ACNGT	5 cut(s)	898, 977, 1094, 1168, 2010
TaiI	ACGT	4 cut(s)	133, 1842, 1885, 2276
TaqII	GACCGA	1 cut(s)	227
TatI	WGTACW	2 cut(s)	3, 1060
TscAI	CASTG	4 cut(s)	128, 808, 982, 1809
TseFI	GTSAC	3 cut(s)	856, 1924, 1997
TseI	GCWGC	2 cut(s)	1265, 2210
Tsp45I	GTSAC	3 cut(s)	856, 1924, 1997
TspDTI	ATGAA	8 cut(s)	347, 579, 759, 879, 1196, 1841, 1844, 1967
TspGWI	ACGGA	2 cut(s)	875, 1577
TspRI	CASTG	4 cut(s)	128, 808, 982, 1809
Van91I	CCANNNNNTGG	1 cut(s)	248
Vha464I	CTTAAG	1 cut(s)	1046
VpaK11BI	GGWCC	6 cut(s)	45, 86, 210, 284, 371, 1585
VspI	ATTAAT	1 cut(s)	891
XapI	RAATTY	2 cut(s)	620, 1289
XceI	RCATGY	1 cut(s)	474
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1301
XhoI	CTCGAG	1 cut(s)	652
XmiI	GTMKAC	2 cut(s)	1811, 1990
XmnI	GAANNNNTTC	2 cut(s)	1167, 2127
XspI	CTAG	8 cut(s)	150, 593, 1056, 1088, 1358, 1490, 1857, 2024
Zsp2I	ATGCAT	2 cut(s)	1105, 2224

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.