Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0012029

Physical Location & Seq

Genomic Coordinates Reverse (-)

51956473 .. 51960346

Gene Structure 3874 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00257630.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2229 bp

ATGTCCTCCGCCCTCATTGAGCGTCCTAGAATCTTCTCGAAGCTTCTAAGCCGGCTCATCCTGACCCAAAACATGGGCGGCGGACCCCGGACCTTCCCCGGCGGCGTCAGCAAGTGGAAATGGAAGCGCATGCACGAGAAACGAGCCAAGGACAAAGAGAAGCGCCTCTTGGACCAAGAAAAGCAGCTCTACGAGGCCCGCCTCCGCTCCCAAATCCGGGGCAGCCTCGTCGGAAAACCCGACCCGCTTTCCGACCCGGGTCCTGAAACCAACCACAGCCCCATGGACCCCGACCGGCACATCAAATCCCTAGCCGATCGGTTCATGAAGGAAGGAGCCGAGGACTTGTGGAACGAAAAGGACGGCCCGGTTCACGAGCAGCCACCGCAGCCGCCGGTCGGGTCGGACCGGCAGTCCAGATCGATTCCGGCTGACCTGAGGAAGCTGATACCGAAAGGTCGAAATTTGGCGGGAAATGATAGAAATTTGAGTAGTTTGGGGTGTAGTTATGTTAGCAGTAGAAGCTATTCGGTTCAGAGTAGAGGGAGGTTTAGGAGGAACGATGATTCGAGTGACGATTCGGATTTCGATTCCGATAATGAGGCCATGCAGCCGTTTTGGGAGGGTAAAAATGGGAATGAGAAACCGAAATCGGAGAGAAATGTGAGGAATTTGGGGAGTAGTGCTTCTCTGGGGAAGTATGATAGGAAGGTGATTAAGAGGAGAGTGCCTCTCAATGCATTGGAAGATGAGTGTGATTTTGCGCAGCAGGTAGAGTCGATTAGGTATGAGCTGAGTAAGAAAAAGGAGGTTGAAATTGAAAGAGGGGAGAGTGAAGAGGAGGGGTCGATTCTTAGTGAGAAGAGATTTGATGAGTGTGGGATATCTCCATTTACAGTCAAGGCATTGTCTTCTGCTGGGTATGTTCGAATGACTAGAGTTCAGGAGGCTGCAATTTCGGCTTGCCTTGAGGGAAAGGATGTTTTGGTCAAAGCTAAAACCGGCACTGGCAAAACTGCTGCCTTTTTGCTTCCGGCTATTGAAGCAGTTGTGAAGGGTATGGCTGGCAATACCAATCAACGGGTGTCTCCAATTTTTGTTCTTATCCTCTGCCCCACAAGAGAACTTGCAAGTCAGATAGCTGCAGAAACAAATGTGTTGTTGAAGTACCATGAAGGCATAGGCATGCAAACATTAGTTGGAGGCACGAGATTTAAAGAGGACCAGAAACGTCTAGAATCAAATCCATGCCAGATAATAGTTGCAACTCCTGGCAGGTTGCTGGACCACATTGAGAATAGGTCTGGCTTGTCGGTGCGATTGATGGGACTGAAAATGTTAATACTTGATGAAGCTGGCCACTTACTAGACCTAGGATTTCGGAAGGACATAGAGAAAATCGTTGATTGTTTGCCTCGTTCGCCGCATATCACAGCTCGTTTTGAAAAAGATCATGCTTTCATTGATACAGTGGGATTGGGTTGTGTGGAAACTCATGCTAAGGTTAAGCAGTCATATCTTGTTGCACCACATGACTTGCATTTTCAAATAGTTCACCATCTTTTGAACGAGCATATCTGGCAATCACCTGATTATAAGGTTATTGTTTTCTGTACAACTGGAATGGTAACATCCCTCCTCTACCTTCTTCTCCGTGAAATGAAAATGAACGTTAGGGAGATACATTCTAGAAAGCCTCAACTGTATCGAACTCGTATCTCTGAGGAATTCAAGGAATCAAAGCAATTGATTCTTGTTACCTCTGATGTATCAGCACGTGGAATGAACTATCCTGATGTTACATTGGTCATTCAGGTGGGTATTCCTTCTGACCGGGAACAATATATACACCGTCTTGGAAGAACAGGACGGGAAGGTAAAGAAGGAGAAGGCATATTGTTGCTTGCACCATGGGAGGAGTATTTTCTGGAGGAATTAAAAGATTTGCCCCTTGAAAAATTTCCTTCACTGCGATTGGATCCAGGCACTAAACTTAAGATTGAGGATTCAATGACCAAGGTTGACAGTAGTGTCAAGGAAGCCGCCTATCATGCTTGGCTTGGCTACTACAACTCGATCAGGGAAACTGGTAGGGATAAAACTACACTTGTTGAGCAAGCCAACCTCTTTTGTCAGTCAATTGGTTTGCAAACTCCTCCTTCTCTCTTTAGAAAGACAGCATTAAAGATGGGTTTGAAAGATATACCTGGCATAAAAATACGAAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

742

Amino Acids

83.78

Weight (kDa)

9.01

Isoelectric Point (pI)

48.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	311 - 468	2.1e-06	Type III restriction enzyme, res subunit
DEAD	PF00270	312 - 472	4.6e-38	DEAD/DEAH box helicase
Helicase_C	PF00271	521 - 626	2.3e-22	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012029)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63250 AT2G07750 AT5G08620
fragaria_vesca	FvH4_5g01920
malus_domestica	MD06G1077400.v1.1
prunus_persica	Prupe.5G095400_v2.0.a1 Prupe.5G095400_v2.0.a1 Prupe.5G095400_v2.0.a1
pyrus_communis	pycom06g07520
rosa_chinensis	RchiOBHm_Chr7g0198601
rosa_laevigata	RLG00000003858
rosa_multiflora	Rmu_sc0004229.1_g000005
rosa_roxburghii	Rroxscaffold_3G00257630
rosa_rugosa	Rorug07G0048400
rosa_samantha	Rh7AG171900 Rh7BG175300 Rh7CG181400 Rh7DG174400
rosa_wichuraiana	Rw7G015080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1596
AasI	GACNNNNNNGTC	1 cut(s)	2030
Acc16I	TGCGCA	1 cut(s)	765
Acc36I	ACCTGC	2 cut(s)	760, 1266
AccB7I	CCANNNNNTGG	1 cut(s)	73
AccBSI	CCGCTC	1 cut(s)	207
AclI	AACGTT	1 cut(s)	1671
AclWI	GGATC	2 cut(s)	1973, 1986
AcoI	YGGCCR	1 cut(s)	1357
AcsI	RAATTY	5 cut(s)	463, 484, 670, 1727, 1958
AcvI	CACGTG	1 cut(s)	1778
AcyI	GRCGYC	1 cut(s)	105
AfaI	GTAC	2 cut(s)	1169, 1615
AfiI	CCNNNNNNNGG	5 cut(s)	73, 216, 217, 398, 1080
AflII	CTTAAG	1 cut(s)	1994
AhdI	GACNNNNNGTC	1 cut(s)	412
AjnI	CCWGG	3 cut(s)	1270, 1981, 2206
AjuI	GAANNNNNNNTTGG	2 cut(s)	152, 184
AluBI	AGCT	9 cut(s)	43, 187, 445, 525, 793, 995, 1142, 1355, 1436
AluI	AGCT	9 cut(s)	43, 187, 445, 525, 793, 995, 1142, 1355, 1436
Alw26I	GTCTC	1 cut(s)	1092
AlwI	GGATC	2 cut(s)	1973, 1986
Ama87I	CYCGRG	1 cut(s)	256
AoxI	GGCC	4 cut(s)	195, 364, 603, 1357
ApeKI	GCWGC	9 cut(s)	184, 222, 379, 388, 610, 766, 950, 1019, 1142
ApoI	RAATTY	5 cut(s)	463, 484, 670, 1727, 1958
Asp700I	GAANNNNTTC	2 cut(s)	526, 1959
AspA2I	CCTAGG	1 cut(s)	1372
AspLEI	GCGC	3 cut(s)	129, 165, 766
AsuC2I	CCSGG	7 cut(s)	88, 99, 218, 257, 258, 368, 1835
AsuHPI	GGTGA	3 cut(s)	724, 1547, 1578
AsuII	TTCGAA	1 cut(s)	928
AvaI	CYCGRG	1 cut(s)	256
AvaII	GGWCC	8 cut(s)	83, 90, 172, 260, 286, 406, 1222, 1285
AvrII	CCTAGG	1 cut(s)	1372
AxyI	CCTNAGG	1 cut(s)	437
BalI	TGGCCA	1 cut(s)	1359
BamHI	GGATCC	1 cut(s)	1978
BauI	CACGAG	3 cut(s)	134, 374, 1207
BbrPI	CACGTG	1 cut(s)	1778
BbsI	GAAGAC	1 cut(s)	903
BbvI	GCAGC	9 cut(s)	196, 234, 391, 400, 622, 778, 937, 1006, 1129
BccI	CCATC	3 cut(s)	1318, 1566, 2182
BceAI	ACGGC	2 cut(s)	379, 598
BcgI	CGANNNNNNTGC	2 cut(s)	211, 245
BciT130I	CCWGG	3 cut(s)	1272, 1983, 2208
BcnI	CCSGG	7 cut(s)	88, 99, 218, 257, 258, 368, 1835
BcoDI	GTCTC	1 cut(s)	1092
BfaI	CTAG	7 cut(s)	27, 311, 936, 1235, 1367, 1373, 1689
BfmI	CTRYAG	1 cut(s)	1143
BfoI	RGCGCY	1 cut(s)	166
BfrI	CTTAAG	1 cut(s)	1994
BfuAI	ACCTGC	2 cut(s)	760, 1266
BlnI	CCTAGG	1 cut(s)	1372
Bme18I	GGWCC	8 cut(s)	83, 90, 172, 260, 286, 406, 1222, 1285
BmeRI	GACNNNNNGTC	1 cut(s)	412
BmeT110I	CYCGRG	1 cut(s)	256
BmiI	GGNNCC	5 cut(s)	85, 261, 288, 337, 1980
BoxI	GACNNNNGTC	1 cut(s)	258
BpiI	GAAGAC	1 cut(s)	903
BplI	GAGNNNNNCTC	4 cut(s)	715, 747, 717, 749
BpmI	CTGGAG	1 cut(s)	1949
Bpu10I	CCTNAGC	1 cut(s)	1500
Bpu14I	TTCGAA	1 cut(s)	928
BpuEI	CTTGAG	1 cut(s)	989
BpuMI	CCSGG	7 cut(s)	88, 99, 218, 257, 258, 368, 1835
Bsa29I	ATCGAT	1 cut(s)	422
BsaAI	YACGTR	1 cut(s)	1778
BsaHI	GRCGYC	1 cut(s)	105
Bsc4I	CCNNNNNNNGG	5 cut(s)	73, 216, 217, 398, 1080
Bse118I	RCCGGY	5 cut(s)	51, 294, 394, 408, 1001
Bse1I	ACTGG	3 cut(s)	1012, 1624, 2092
Bse21I	CCTNAGG	1 cut(s)	437
BseBI	CCWGG	3 cut(s)	1272, 1983, 2208
BseCI	ATCGAT	1 cut(s)	422
BseGI	GGATG	3 cut(s)	57, 985, 1631
BseLI	CCNNNNNNNGG	5 cut(s)	73, 216, 217, 398, 1080
BseMII	CTCAG	3 cut(s)	428, 785, 1713
BseNI	ACTGG	3 cut(s)	1012, 1624, 2092
BseRI	GAGGAG	5 cut(s)	736, 854, 1628, 1931, 2145
BseXI	GCAGC	9 cut(s)	196, 234, 391, 400, 622, 778, 937, 1006, 1129
BseYI	CCCAGC	1 cut(s)	917
Bsh1285I	CGRYCG	3 cut(s)	295, 319, 399
BshFI	GGCC	4 cut(s)	197, 366, 605, 1359
BshVI	ATCGAT	1 cut(s)	422
BsiEI	CGRYCG	3 cut(s)	295, 319, 399
BsiHKCI	CYCGRG	1 cut(s)	256
BslFI	GGGAC	1 cut(s)	1341
BslI	CCNNNNNNNGG	5 cut(s)	73, 216, 217, 398, 1080
BsmAI	GTCTC	1 cut(s)	1092
BsmFI	GGGAC	1 cut(s)	1341
BsnI	GGCC	4 cut(s)	197, 366, 605, 1359
BsoBI	CYCGRG	1 cut(s)	256
Bsp119I	TTCGAA	1 cut(s)	928
Bsp1407I	TGTACA	1 cut(s)	1613
Bsp143I	GATC	5 cut(s)	316, 419, 1450, 1978, 2076
Bsp19I	CCATGG	2 cut(s)	282, 1910
BspANI	GGCC	4 cut(s)	197, 366, 605, 1359
BspCNI	CTCAG	3 cut(s)	429, 786, 1714
BspDI	ATCGAT	1 cut(s)	422
BspHI	TCATGA	1 cut(s)	324
BspLI	GGNNCC	5 cut(s)	85, 261, 288, 337, 1980
BspMAI	CTGCAG	1 cut(s)	1147
BspMI	ACCTGC	2 cut(s)	760, 1266
BspPI	GGATC	2 cut(s)	1973, 1986
BspT104I	TTCGAA	1 cut(s)	928
BspTI	CTTAAG	1 cut(s)	1994
BsrBI	CCGCTC	1 cut(s)	207
BsrFI	RCCGGY	5 cut(s)	51, 294, 394, 408, 1001
BsrGI	TGTACA	1 cut(s)	1613
BsrI	ACTGG	3 cut(s)	1012, 1624, 2092
BssAI	RCCGGY	5 cut(s)	51, 294, 394, 408, 1001
BssMI	GATC	5 cut(s)	316, 419, 1450, 1978, 2076
BssNI	GRCGYC	1 cut(s)	105
BssSI	CACGAG	3 cut(s)	134, 374, 1207
BssT1I	CCWWGG	5 cut(s)	147, 282, 1372, 1910, 2016
Bst2BI	CACGAG	3 cut(s)	134, 374, 1207
Bst2UI	CCWGG	3 cut(s)	1272, 1983, 2208
Bst4CI	ACNGT	5 cut(s)	898, 1471, 1704, 1853, 2027
Bst6I	CTCTTC	2 cut(s)	831, 857
BstACI	GRCGYC	1 cut(s)	105
BstAFI	CTTAAG	1 cut(s)	1994
BstAUI	TGTACA	1 cut(s)	1613
BstBAI	YACGTR	1 cut(s)	1778
BstBI	TTCGAA	1 cut(s)	928
BstC8I	GCNNGC	9 cut(s)	53, 131, 199, 964, 1066, 1187, 1357, 1905, 2118
BstDEI	CTNAG	6 cut(s)	47, 437, 794, 854, 1500, 1722
BstDSI	CCRYGG	2 cut(s)	282, 1910
BstF5I	GGATG	3 cut(s)	57, 985, 1631
BstH2I	RGCGCY	1 cut(s)	166
BstHHI	GCGC	3 cut(s)	129, 165, 766
BstKTI	GATC	5 cut(s)	319, 422, 1453, 1981, 2079
BstMAI	GTCTC	1 cut(s)	1092
BstMBI	GATC	5 cut(s)	316, 419, 1450, 1978, 2076
BstMCI	CGRYCG	3 cut(s)	295, 319, 399
BstMWI	GCNNNNNNNGC	9 cut(s)	108, 385, 388, 522, 959, 1043, 1420, 1579, 2051
BstNI	CCWGG	3 cut(s)	1272, 1983, 2208
BstNSI	RCATGY	2 cut(s)	133, 1189
BstPAI	GACNNNNGTC	1 cut(s)	258
BstSFI	CTRYAG	1 cut(s)	1143
BstV1I	GCAGC	9 cut(s)	196, 234, 391, 400, 622, 778, 937, 1006, 1129
BstV2I	GAAGAC	1 cut(s)	903
BstX2I	RGATCY	1 cut(s)	1978
BstYI	RGATCY	1 cut(s)	1978
Bsu15I	ATCGAT	1 cut(s)	422
Bsu36I	CCTNAGG	1 cut(s)	437
BsuRI	GGCC	4 cut(s)	197, 366, 605, 1359
BsuTUI	ATCGAT	1 cut(s)	422
BtgI	CCRYGG	2 cut(s)	282, 1910
BtsCI	GGATG	3 cut(s)	57, 985, 1631
BtsI	GCAGTG	1 cut(s)	1967
BtsIMutI	CAGTG	3 cut(s)	1005, 1476, 1967
BveI	ACCTGC	2 cut(s)	760, 1266
Cac8I	GCNNGC	9 cut(s)	53, 131, 199, 964, 1066, 1187, 1357, 1905, 2118
CciI	TCATGA	1 cut(s)	324
CfoI	GCGC	3 cut(s)	129, 165, 766
Cfr10I	RCCGGY	5 cut(s)	51, 294, 394, 408, 1001
Cfr9I	CCCGGG	1 cut(s)	256
ClaI	ATCGAT	1 cut(s)	422
CpoI	CGGWCCG	1 cut(s)	406
CseI	GACGC	2 cut(s)	11, 94
Csp6I	GTAC	2 cut(s)	1168, 1614
CspCI	CAANNNNNGTGG	2 cut(s)	1518, 1553
CspI	CGGWCCG	1 cut(s)	406
CviQI	GTAC	2 cut(s)	1168, 1614
DdeI	CTNAG	6 cut(s)	47, 437, 794, 854, 1500, 1722
DpnI	GATC	5 cut(s)	318, 421, 1452, 1980, 2078
DpnII	GATC	5 cut(s)	316, 419, 1450, 1978, 2076
DraI	TTTAAA	1 cut(s)	1216
DrdI	GACNNNNNNGTC	1 cut(s)	2030
DriI	GACNNNNNGTC	1 cut(s)	412
DseDI	GACNNNNNNGTC	1 cut(s)	2030
EaeI	YGGCCR	1 cut(s)	1357
Eam1104I	CTCTTC	2 cut(s)	831, 857
Eam1105I	GACNNNNNGTC	1 cut(s)	412
EarI	CTCTTC	2 cut(s)	831, 857
EciI	GGCGGA	1 cut(s)	96
Eco130I	CCWWGG	5 cut(s)	147, 282, 1372, 1910, 2016
Eco32I	GATATC	1 cut(s)	885
Eco47I	GGWCC	8 cut(s)	83, 90, 172, 260, 286, 406, 1222, 1285
Eco72I	CACGTG	1 cut(s)	1778
Eco81I	CCTNAGG	1 cut(s)	437
Eco88I	CYCGRG	1 cut(s)	256
EcoO109I	RGGNCCY	1 cut(s)	260
EcoRI	GAATTC	1 cut(s)	1727
EcoRII	CCWGG	3 cut(s)	1270, 1981, 2206
EcoRV	GATATC	1 cut(s)	885
EcoT14I	CCWWGG	5 cut(s)	147, 282, 1372, 1910, 2016
EcoT22I	ATGCAT	1 cut(s)	742
ErhI	CCWWGG	5 cut(s)	147, 282, 1372, 1910, 2016
FalI	AAGNNNNNCTT	2 cut(s)	152, 184
FaqI	GGGAC	1 cut(s)	1341
FauI	CCCGC	3 cut(s)	206, 252, 463
FokI	GGATG	3 cut(s)	44, 992, 1618
FspBI	CTAG	7 cut(s)	27, 311, 936, 1235, 1367, 1373, 1689
FspI	TGCGCA	1 cut(s)	765
GlaI	GCGC	3 cut(s)	128, 164, 765
GsaI	CCCAGC	1 cut(s)	921
GsuI	CTGGAG	1 cut(s)	1949
HaeII	RGCGCY	1 cut(s)	166
HaeIII	GGCC	4 cut(s)	197, 366, 605, 1359
HgaI	GACGC	2 cut(s)	11, 94
HhaI	GCGC	3 cut(s)	129, 165, 766
Hin1I	GRCGYC	1 cut(s)	105
Hin6I	GCGC	3 cut(s)	127, 163, 764
HinP1I	GCGC	3 cut(s)	127, 163, 764
HincII	GTYRAC	1 cut(s)	2023
HindII	GTYRAC	1 cut(s)	2023
HindIII	AAGCTT	1 cut(s)	41
HphI	GGTGA	3 cut(s)	724, 1547, 1578
Hpy166II	GTNNAC	3 cut(s)	373, 1555, 2023
Hpy8I	GTNNAC	3 cut(s)	373, 1555, 2023
Hpy99I	CGWCG	1 cut(s)	233
HpyCH4III	ACNGT	5 cut(s)	898, 1471, 1704, 1853, 2027
HpyCH4IV	ACGT	3 cut(s)	1231, 1671, 1777
HpyF10VI	GCNNNNNNNGC	9 cut(s)	108, 385, 388, 522, 959, 1043, 1420, 1579, 2051
HpyF3I	CTNAG	6 cut(s)	47, 437, 794, 854, 1500, 1722
HpySE526I	ACGT	3 cut(s)	1231, 1671, 1777
Hsp92I	GRCGYC	1 cut(s)	105
HspAI	GCGC	3 cut(s)	127, 163, 764
KroI	GCCGGC	1 cut(s)	51
KroNI	GCCGGC	1 cut(s)	53
Kzo9I	GATC	5 cut(s)	316, 419, 1450, 1978, 2076
LmnI	GCTCC	2 cut(s)	212, 335
Lsp1109I	GCAGC	9 cut(s)	196, 234, 391, 400, 622, 778, 937, 1006, 1129
MaeI	CTAG	7 cut(s)	27, 311, 936, 1235, 1367, 1373, 1689
MaeII	ACGT	3 cut(s)	1231, 1671, 1777
MaeIII	GTNAC	4 cut(s)	572, 1627, 1756, 1798
MalI	GATC	5 cut(s)	318, 421, 1452, 1980, 2078
MbiI	CCGCTC	1 cut(s)	207
MboI	GATC	5 cut(s)	316, 419, 1450, 1978, 2076
MboII	GAAGA	7 cut(s)	25, 758, 848, 874, 903, 1640, 1872
MfeI	CAATTG	2 cut(s)	1745, 2139
MflI	RGATCY	1 cut(s)	1978
MlsI	TGGCCA	1 cut(s)	1359
MluNI	TGGCCA	1 cut(s)	1359
MlyI	GAGTC	1 cut(s)	785
MmeI	TCCRAC	4 cut(s)	211, 276, 384, 1180
Mox20I	TGGCCA	1 cut(s)	1359
Mph1103I	ATGCAT	1 cut(s)	742
MroNI	GCCGGC	1 cut(s)	51
MroXI	GAANNNNTTC	2 cut(s)	526, 1959
MscI	TGGCCA	1 cut(s)	1359
MseI	TTAA	7 cut(s)	717, 1215, 1340, 1506, 1937, 1995, 2183
MslI	CAYNNNNRTG	2 cut(s)	1184, 1814
Msp20I	TGGCCA	1 cut(s)	1359
MspCI	CTTAAG	1 cut(s)	1994
MunI	CAATTG	2 cut(s)	1745, 2139
MvaI	CCWGG	3 cut(s)	1272, 1983, 2208
MwoI	GCNNNNNNNGC	9 cut(s)	108, 385, 388, 522, 959, 1043, 1420, 1579, 2051
NaeI	GCCGGC	1 cut(s)	53
NciI	CCSGG	7 cut(s)	88, 99, 218, 257, 258, 368, 1835
NcoI	CCATGG	2 cut(s)	282, 1910
NdeII	GATC	5 cut(s)	316, 419, 1450, 1978, 2076
NgoMIV	GCCGGC	1 cut(s)	51
NlaIV	GGNNCC	5 cut(s)	85, 261, 288, 337, 1980
NmeAIII	GCCGAG	1 cut(s)	364
NmuCI	GTSAC	1 cut(s)	572
NsbI	TGCGCA	1 cut(s)	765
NsiI	ATGCAT	1 cut(s)	742
NspI	RCATGY	2 cut(s)	133, 1189
NspV	TTCGAA	1 cut(s)	928
PaeI	GCATGC	2 cut(s)	133, 1189
PagI	TCATGA	1 cut(s)	324
PcsI	WCGNNNNNNNCGW	1 cut(s)	237
PdiI	GCCGGC	1 cut(s)	53
PdmI	GAANNNNTTC	2 cut(s)	526, 1959
PflMI	CCANNNNNTGG	1 cut(s)	73
Ple19I	CGATCG	1 cut(s)	319
PleI	GAGTC	1 cut(s)	784
PmaCI	CACGTG	1 cut(s)	1778
PmlI	CACGTG	1 cut(s)	1778
PpsI	GAGTC	1 cut(s)	784
Ppu21I	YACGTR	1 cut(s)	1778
PpuMI	RGGWCCY	1 cut(s)	260
PshAI	GACNNNNGTC	1 cut(s)	258
PsiI	TTATAA	1 cut(s)	1596
Psp1406I	AACGTT	1 cut(s)	1671
Psp5II	RGGWCCY	1 cut(s)	260
Psp6I	CCWGG	3 cut(s)	1270, 1981, 2206
PspCI	CACGTG	1 cut(s)	1778
PspFI	CCCAGC	1 cut(s)	917
PspGI	CCWGG	3 cut(s)	1270, 1981, 2206
PspN4I	GGNNCC	5 cut(s)	85, 261, 288, 337, 1980
PspPPI	RGGWCCY	1 cut(s)	260
PsrI	GAACNNNNNNTAC	2 cut(s)	1830, 1862
PstI	CTGCAG	1 cut(s)	1147
PsuI	RGATCY	1 cut(s)	1978
PvuI	CGATCG	1 cut(s)	319
RsaI	GTAC	2 cut(s)	1169, 1615
RsaNI	GTAC	2 cut(s)	1168, 1614
RseI	CAYNNNNRTG	2 cut(s)	1184, 1814
Rsr2I	CGGWCCG	1 cut(s)	406
RsrII	CGGWCCG	1 cut(s)	406
SaqAI	TTAA	7 cut(s)	717, 1215, 1340, 1506, 1937, 1995, 2183
Sau3AI	GATC	5 cut(s)	316, 419, 1450, 1978, 2076
SchI	GAGTC	1 cut(s)	785
SfcI	CTRYAG	1 cut(s)	1143
SfuI	TTCGAA	1 cut(s)	928
SinI	GGWCC	8 cut(s)	83, 90, 172, 260, 286, 406, 1222, 1285
SmaI	CCCGGG	1 cut(s)	258
SmiMI	CAYNNNNRTG	2 cut(s)	1184, 1814
SmlI	CTYRAG	2 cut(s)	968, 1994
SmoI	CTYRAG	2 cut(s)	968, 1994
SphI	GCATGC	2 cut(s)	133, 1189
SspMI	CTAG	7 cut(s)	27, 311, 936, 1235, 1367, 1373, 1689
StyI	CCWWGG	5 cut(s)	147, 282, 1372, 1910, 2016
TaaI	ACNGT	5 cut(s)	898, 1471, 1704, 1853, 2027
TaiI	ACGT	3 cut(s)	1234, 1674, 1780
TatI	WGTACW	1 cut(s)	1613
TauI	GCSGC	5 cut(s)	81, 105, 394, 1426, 2045
Tru1I	TTAA	7 cut(s)	717, 1215, 1340, 1506, 1937, 1995, 2183
Tru9I	TTAA	7 cut(s)	717, 1215, 1340, 1506, 1937, 1995, 2183
TscAI	CASTG	3 cut(s)	1012, 1476, 1974
TseFI	GTSAC	1 cut(s)	572
TseI	GCWGC	9 cut(s)	184, 222, 379, 388, 610, 766, 950, 1019, 1142
Tsp45I	GTSAC	1 cut(s)	572
TspDTI	ATGAA	8 cut(s)	313, 341, 1188, 1365, 1450, 1676, 1682, 1799
TspGWI	ACGGA	1 cut(s)	1643
TspMI	CCCGGG	1 cut(s)	256
TspRI	CASTG	3 cut(s)	1012, 1476, 1974
Van91I	CCANNNNNTGG	1 cut(s)	73
Vha464I	CTTAAG	1 cut(s)	1994
VpaK11BI	GGWCC	8 cut(s)	83, 90, 172, 260, 286, 406, 1222, 1285
XapI	RAATTY	5 cut(s)	463, 484, 670, 1727, 1958
XbaI	TCTAGA	2 cut(s)	1234, 1688
XceI	RCATGY	2 cut(s)	133, 1189
XmaI	CCCGGG	1 cut(s)	256
XmaJI	CCTAGG	1 cut(s)	1372
XmnI	GAANNNNTTC	2 cut(s)	526, 1959
XspI	CTAG	7 cut(s)	27, 311, 936, 1235, 1367, 1373, 1689
Zsp2I	ATGCAT	1 cut(s)	742

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.