Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0012029

Physical Location & Seq

Genomic Coordinates Reverse (-)

19214981 .. 19219616

Gene Structure 4636 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1077400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2265 bp

ATGTCTGCCTCGATGCTTATGGAGCGTCCGAGACAGTTCTCCAAGCTTCTCTGCAGGCTCATTCTAACCCGACCCATGGGCGGCGGGCCTCGGACCTTCCCGGGCGGCGTCAGCAAGTGGAAATGGAAGCGCATGCACGAAAAGCGAGCCAAAGAGAAAGAGAAACGCCTTTTAGACCAAGAGAAACAGTTATACGAGGCCCGCCTCCGCTCCAAAATTCGGGCCAAAGTCGCCGGAAAGCCCGACCCGTTTTCGGGCACTGGAACCGGGCACAACCCGATGAACCCGAACCAGCACCTGAAGGTACTTGCCGATCGGTTCATGAAGGAAGGCGCAGAAGATTTGTGGAACGAAAAGGACGGGCCGATTGTTGAACCGCCCCCGTCGGTTCGACCGGACGGAACGACGAGATCGATTGCGCCGCCGCCACTGGACCTGAGAAAGCTGATATCGAAAGGTCGTGATTTGGCGGGAAAGAGGGACAGTGTAAATTTGAGTAATTTGGGTGGCAATTATGTTGGGAGCAGGAACTACTCGGCGCAGAGTCGAGGGCGATTTCGGAGAAATGATAGTTCTTCCGACGACTCGGATTTTGATTCGGACGGTGAGTCCGTACAGCCGTTTTCGAATGAGAATTCGAAATTTGGGAGAAATGTGATGAGATTTGGGAGTAGTGCTTCATTGGGAAAGTATGATATGAAGATCATTAAGAGGAGAGTGCCCCTTAATTCACTGGATGACGAAAATGATTTCCAACAGCAGGTGGAGGTGATCCGAAGCGAGCTCAATAAGAAAAAAGCCGTTGAAATCGAAAGAGAGGAGAGCCAAAAGGAGGTGTCAATTCTTAGCGAGAAGAGATTTGATGAGTGTGGTATATCTCCATTGACAGTCAAGGCGCTCACTTCTGCCGGGTATGTTAAAATGACAAGGGTACAGGAGGTTGCTCTTTCTGTTTGCCTTGAAGGAAAAGATGCGTTGGTGAAAGCTAAAACAGGCACAGGCAAAACTGCAGCTTTTTTGCTTCCTGCTATTGAAGCAGTTGTGAAGGCTATGGCTAGCAATACCAATCAACGGGTGTCTCCAGTTTTTGTTCTTATCCTCTGTCCCACAAGAGAACTCGCAAGTCAGATAGCTGCAGAGACAAATGTTTTATTGCGGTACCATGATGGCATAGGTATGCAAACACTAGTTGGAGGCACGCGATTCAAAGAAGACCAGAAATGTCTAGAATCAAATCCATGCCAGATAATAGTTGCAACCCCTGGCAGGTTGCTAGACCACATTGAGAATAAATCAGGCTTGTCTGTGCGGTTGATGGGACTGAAAATGCTAATACTCGATGAAGCGGGCTACTTACTAGACCTAGGATTTCGAAAGGATATAGAGAAAATTGTTGACTGTTTGCCTCGTCGAAGGCAGTCCTTGCTCTTTACTGCAACACTTCCAAAAGAGGTTCGCCGAGTATCACAGCTTGTTTTGCAAAAGGACCATATTTTCGTTGACACAGTGGGTTTAGGTTCTGTTGAAACTCATGCTAAGGTTAAGCAGTCATGTTTTGTTGTGCAACATGAATTACATTTCCAAATAGTGCACCAGCTTCTGATGGAACATATCTCTGAGTCACCTGATTATAAGGTTATTGTTTTCTGTACAACTGGAATGGTAACATCCCTCCTCTATCATGTTCTCCATGAAATGAAAATGAATGTCAAGGAGATACATTCTAGGAAGCCTCAACTCTATCGAACTCGCATCTCTGAGGAATTCAGGGTATCGAAACGACTGATTCTTGTTACCTCTGATGTATCGGCACGTGGAATGAACTATCCTGATGTGACCTTGGTCATTCAGGTAGGTATTCCTTCTGACCGGGAACAATATATAAATCGTCTTGGAAGAACAGGAAGGGAAGGCAAAGAAGGACGAGGCATATTGTTACTTGCACCATGGGAGGAGTATTTTCTGGATGAATTAAAAGATTTGCCTTTGGAAAAATTTCCTTCGCTGCAATTGGATCGGGATACTAAGCTTAAGATTGACGATTCGATGACCAAGATTGACACTAGCATCAAAGAAGCGGCATTTCATGCGTGGCTTGGGTATTATAACTCGATCAGGGAAACGGGCAGGGATAAGACCACACTTGTCGAGCAAGCCAACAAATTTTGTGAGTCAATTGGTTTGCAAAAACCTCCTTCTCTCTTTAGAAAGACAGCCTTGAAGATGGGTTTGAAAGACATACCTGGCATAAGAATACGAAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

755

Amino Acids

85.16

Weight (kDa)

9.49

Isoelectric Point (pI)

45.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	309 - 487	4.3e-41	DEAD/DEAH box helicase
ResIII	PF04851	321 - 482	1.3e-06	Type III restriction enzyme, res subunit
Helicase_C	PF00271	533 - 638	3.8e-20	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012029)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63250 AT2G07750 AT5G08620
fragaria_vesca	FvH4_5g01920
malus_domestica	MD06G1077400.v1.1
prunus_persica	Prupe.5G095400_v2.0.a1 Prupe.5G095400_v2.0.a1 Prupe.5G095400_v2.0.a1
pyrus_communis	pycom06g07520
rosa_chinensis	RchiOBHm_Chr7g0198601
rosa_laevigata	RLG00000003858
rosa_multiflora	Rmu_sc0004229.1_g000005
rosa_roxburghii	Rroxscaffold_3G00257630
rosa_rugosa	Rorug07G0048400
rosa_samantha	Rh7AG171900 Rh7BG175300 Rh7CG181400 Rh7DG174400
rosa_wichuraiana	Rw7G015080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1632, 2106
AarI	CACCTGC	1 cut(s)	751
Acc36I	ACCTGC	2 cut(s)	751, 1257
Acc65I	GGTACC	1 cut(s)	1158
AccB1I	GGYRCC	1 cut(s)	1158
AccBSI	CCGCTC	1 cut(s)	210
AccII	CGCG	1 cut(s)	1201
AclWI	GGATC	2 cut(s)	766, 2022
AcsI	RAATTY	7 cut(s)	216, 490, 634, 641, 1763, 1994, 2162
AcuI	CTGAAG	1 cut(s)	320
AcvI	CACGTG	1 cut(s)	1814
AcyI	GRCGYC	1 cut(s)	108
AfaI	GTAC	5 cut(s)	306, 615, 933, 1160, 1651
AfiI	CCNNNNNNNGG	9 cut(s)	76, 80, 219, 253, 254, 760, 832, 1071, 1266
AflII	CTTAAG	1 cut(s)	2030
AgsI	TTSAA	8 cut(s)	374, 806, 962, 1034, 1207, 1526, 2221, 2233
AhdI	GACNNNNNGTC	1 cut(s)	607
AhlI	ACTAGT	1 cut(s)	1186
AjnI	CCWGG	2 cut(s)	1261, 2242
AluBI	AGCT	9 cut(s)	46, 445, 784, 986, 1013, 1133, 1471, 1597, 2029
AluI	AGCT	9 cut(s)	46, 445, 784, 986, 1013, 1133, 1471, 1597, 2029
Alw21I	GWGCWC	2 cut(s)	786, 1593
Alw26I	GTCTC	3 cut(s)	25, 1083, 1133
Alw44I	GTGCAC	1 cut(s)	1589
AlwI	GGATC	2 cut(s)	766, 2022
AlwNI	CAGNNNCTG	2 cut(s)	298, 1600
Ama87I	CYCGRG	1 cut(s)	100
AoxI	GGCC	4 cut(s)	86, 198, 222, 362
ApaLI	GTGCAC	1 cut(s)	1589
ApeKI	GCWGC	3 cut(s)	1010, 1133, 2005
ApoI	RAATTY	7 cut(s)	216, 490, 634, 641, 1763, 1994, 2162
ArsI	GACNNNNNNTTYG	4 cut(s)	575, 607, 1478, 1510
Asp700I	GAANNNNTTC	1 cut(s)	1995
Asp718I	GGTACC	1 cut(s)	1158
AspA2I	CCTAGG	1 cut(s)	1363
AspLEI	GCGC	5 cut(s)	132, 335, 421, 541, 898
AspS9I	GGNCC	7 cut(s)	86, 93, 199, 222, 362, 433, 1486
AsuC2I	CCSGG	5 cut(s)	101, 102, 268, 910, 1871
AsuHPI	GGTGA	4 cut(s)	617, 781, 991, 1614
AsuII	TTCGAA	3 cut(s)	626, 638, 1372
AsuNHI	GCTAGC	1 cut(s)	1055
AvaI	CYCGRG	1 cut(s)	100
AvaII	GGWCC	3 cut(s)	93, 433, 1486
AvrII	CCTAGG	1 cut(s)	1363
BaeGI	GKGCMC	4 cut(s)	260, 273, 723, 1593
BanI	GGYRCC	1 cut(s)	1158
BanII	GRGCYC	1 cut(s)	786
BbrPI	CACGTG	1 cut(s)	1814
BbsI	GAAGAC	1 cut(s)	1218
Bbv12I	GWGCWC	2 cut(s)	786, 1593
BbvI	GCAGC	3 cut(s)	1022, 1120, 1992
BccI	CCATC	4 cut(s)	1160, 1309, 1597, 2218
BceAI	ACGGC	2 cut(s)	604, 785
BcgI	CGANNNNNNTGC	2 cut(s)	1997, 2031
BciT130I	CCWGG	2 cut(s)	1263, 2244
BciVI	GTATCC	1 cut(s)	2014
BcnI	CCSGG	5 cut(s)	101, 102, 268, 910, 1871
BcoDI	GTCTC	3 cut(s)	25, 1083, 1133
BcuI	ACTAGT	1 cut(s)	1186
BfaI	CTAG	8 cut(s)	1056, 1187, 1226, 1274, 1358, 1364, 1725, 2064
BfmI	CTRYAG	3 cut(s)	52, 1008, 1134
BfoI	RGCGCY	1 cut(s)	899
BfrI	CTTAAG	1 cut(s)	2030
BfuAI	ACCTGC	2 cut(s)	751, 1257
BfuI	GTATCC	1 cut(s)	2014
BisI	GCNGC	8 cut(s)	82, 106, 422, 425, 1011, 1134, 2006, 2079
BlnI	CCTAGG	1 cut(s)	1363
BlsI	GCNGC	8 cut(s)	83, 107, 423, 426, 1012, 1135, 2007, 2080
Bme1390I	CCNGG	7 cut(s)	101, 102, 268, 910, 1263, 1871, 2244
Bme18I	GGWCC	3 cut(s)	93, 433, 1486
BmeRI	GACNNNNNGTC	1 cut(s)	607
BmeT110I	CYCGRG	1 cut(s)	100
BmgT120I	GGNCC	7 cut(s)	86, 93, 199, 222, 362, 433, 1486
BmiI	GGNNCC	2 cut(s)	265, 1160
BmrFI	CCNGG	7 cut(s)	101, 102, 268, 910, 1263, 1871, 2244
BmsI	GCATC	4 cut(s)	3, 961, 1761, 2076
BmtI	GCTAGC	1 cut(s)	1059
BoxI	GACNNNNGTC	1 cut(s)	1841
BpiI	GAAGAC	1 cut(s)	1218
BpmI	CTGGAG	1 cut(s)	1065
Bpu10I	CCTNAGC	1 cut(s)	1536
Bpu14I	TTCGAA	3 cut(s)	626, 638, 1372
BpuMI	CCSGG	5 cut(s)	101, 102, 268, 910, 1871
Bsa29I	ATCGAT	1 cut(s)	413
BsaAI	YACGTR	1 cut(s)	1814
BsaHI	GRCGYC	1 cut(s)	108
BsaJI	CCNNGG	7 cut(s)	75, 89, 100, 1261, 1363, 1839, 1946
BsaWI	WCCGGW	1 cut(s)	394
Bsc4I	CCNNNNNNNGG	9 cut(s)	76, 80, 219, 253, 254, 760, 832, 1071, 1266
Bse1I	ACTGG	5 cut(s)	265, 435, 738, 1082, 1660
BseBI	CCWGG	2 cut(s)	1263, 2244
BseCI	ATCGAT	1 cut(s)	413
BseDI	CCNNGG	7 cut(s)	75, 89, 100, 1261, 1363, 1839, 1946
BseGI	GGATG	3 cut(s)	742, 1667, 1972
BseLI	CCNNNNNNNGG	9 cut(s)	76, 80, 219, 253, 254, 760, 832, 1071, 1266
BseMII	CTCAG	3 cut(s)	428, 1608, 1749
BseNI	ACTGG	5 cut(s)	265, 435, 738, 1082, 1660
BseRI	GAGGAG	4 cut(s)	727, 833, 1664, 1967
BseSI	GKGCMC	4 cut(s)	260, 273, 723, 1593
BseXI	GCAGC	3 cut(s)	1022, 1120, 1992
Bsh1236I	CGCG	1 cut(s)	1201
Bsh1285I	CGRYCG	2 cut(s)	316, 395
BshFI	GGCC	4 cut(s)	88, 200, 224, 364
BshNI	GGYRCC	1 cut(s)	1158
BshVI	ATCGAT	1 cut(s)	413
BsiEI	CGRYCG	2 cut(s)	316, 395
BsiHKAI	GWGCWC	2 cut(s)	786, 1593
BsiHKCI	CYCGRG	1 cut(s)	100
BsiSI	CCGG	6 cut(s)	101, 234, 267, 395, 909, 1870
BslFI	GGGAC	3 cut(s)	494, 1089, 1332
BslI	CCNNNNNNNGG	9 cut(s)	76, 80, 219, 253, 254, 760, 832, 1071, 1266
BsmAI	GTCTC	3 cut(s)	25, 1083, 1133
BsmFI	GGGAC	3 cut(s)	494, 1089, 1332
BsnI	GGCC	4 cut(s)	88, 200, 224, 364
BsoBI	CYCGRG	1 cut(s)	100
Bsp119I	TTCGAA	3 cut(s)	626, 638, 1372
Bsp1286I	GDGCHC	5 cut(s)	260, 273, 723, 786, 1593
Bsp1407I	TGTACA	1 cut(s)	1649
Bsp143I	GATC	6 cut(s)	313, 410, 702, 771, 2014, 2112
Bsp19I	CCATGG	2 cut(s)	75, 1946
BspANI	GGCC	4 cut(s)	88, 200, 224, 364
BspCNI	CTCAG	3 cut(s)	429, 1609, 1750
BspDI	ATCGAT	1 cut(s)	413
BspFNI	CGCG	1 cut(s)	1201
BspHI	TCATGA	1 cut(s)	321
BspLI	GGNNCC	2 cut(s)	265, 1160
BspMAI	CTGCAG	3 cut(s)	56, 1012, 1138
BspMI	ACCTGC	2 cut(s)	751, 1257
BspOI	GCTAGC	1 cut(s)	1059
BspPI	GGATC	2 cut(s)	766, 2022
BspT104I	TTCGAA	3 cut(s)	626, 638, 1372
BspT107I	GGYRCC	1 cut(s)	1158
BspTI	CTTAAG	1 cut(s)	2030
BsrBI	CCGCTC	1 cut(s)	210
BsrGI	TGTACA	1 cut(s)	1649
BsrI	ACTGG	5 cut(s)	265, 435, 738, 1082, 1660
BssECI	CCNNGG	7 cut(s)	75, 89, 100, 1261, 1363, 1839, 1946
BssMI	GATC	6 cut(s)	313, 410, 702, 771, 2014, 2112
BssNI	GRCGYC	1 cut(s)	108
BssT1I	CCWWGG	4 cut(s)	75, 1363, 1839, 1946
Bst2UI	CCWGG	2 cut(s)	1263, 2244
Bst4CI	ACNGT	7 cut(s)	36, 189, 485, 605, 889, 1400, 1507
Bst6I	CTCTTC	1 cut(s)	848
BstACI	GRCGYC	1 cut(s)	108
BstAFI	CTTAAG	1 cut(s)	2030
BstAPI	GCANNNNNTGC	2 cut(s)	1423, 2087
BstAUI	TGTACA	1 cut(s)	1649
BstBAI	YACGTR	1 cut(s)	1814
BstBI	TTCGAA	3 cut(s)	626, 638, 1372
BstDEI	CTNAG	6 cut(s)	437, 845, 1536, 1617, 1758, 2025
BstDSI	CCRYGG	2 cut(s)	75, 1946
BstF5I	GGATG	3 cut(s)	742, 1667, 1972
BstFNI	CGCG	1 cut(s)	1201
BstH2I	RGCGCY	1 cut(s)	899
BstHHI	GCGC	5 cut(s)	132, 335, 421, 541, 898
BstKTI	GATC	6 cut(s)	316, 413, 705, 774, 2017, 2115
BstMAI	GTCTC	3 cut(s)	25, 1083, 1133
BstMBI	GATC	6 cut(s)	313, 410, 702, 771, 2014, 2112
BstMCI	CGRYCG	2 cut(s)	316, 395
BstMWI	GCNNNNNNNGC	8 cut(s)	22, 111, 142, 230, 1034, 1423, 1477, 2087
BstNI	CCWGG	2 cut(s)	1263, 2244
BstNSI	RCATGY	1 cut(s)	136
BstPAI	GACNNNNGTC	1 cut(s)	1841
BstSCI	CCNGG	7 cut(s)	99, 100, 266, 908, 1261, 1869, 2242
BstSFI	CTRYAG	3 cut(s)	52, 1008, 1134
BstSLI	GKGCMC	4 cut(s)	260, 273, 723, 1593
BstUI	CGCG	1 cut(s)	1201
BstV1I	GCAGC	3 cut(s)	1022, 1120, 1992
BstV2I	GAAGAC	1 cut(s)	1218
Bsu15I	ATCGAT	1 cut(s)	413
BsuI	GTATCC	1 cut(s)	2014
BsuRI	GGCC	4 cut(s)	88, 200, 224, 364
BsuTUI	ATCGAT	1 cut(s)	413
BtgI	CCRYGG	2 cut(s)	75, 1946
BtsCI	GGATG	3 cut(s)	742, 1667, 1972
BtsIMutI	CAGTG	5 cut(s)	258, 428, 490, 731, 1512
BveI	ACCTGC	2 cut(s)	751, 1257
CaiI	CAGNNNCTG	2 cut(s)	298, 1600
CciI	TCATGA	1 cut(s)	321
CfoI	GCGC	5 cut(s)	132, 335, 421, 541, 898
Cfr13I	GGNCC	7 cut(s)	86, 93, 199, 222, 362, 433, 1486
Cfr9I	CCCGGG	1 cut(s)	100
ClaI	ATCGAT	1 cut(s)	413
CseI	GACGC	2 cut(s)	14, 97
Csp6I	GTAC	5 cut(s)	305, 614, 932, 1159, 1650
CviQI	GTAC	5 cut(s)	305, 614, 932, 1159, 1650
DdeI	CTNAG	6 cut(s)	437, 845, 1536, 1617, 1758, 2025
DpnI	GATC	6 cut(s)	315, 412, 704, 773, 2016, 2114
DpnII	GATC	6 cut(s)	313, 410, 702, 771, 2014, 2112
DriI	GACNNNNNGTC	1 cut(s)	607
Eam1104I	CTCTTC	1 cut(s)	848
Eam1105I	GACNNNNNGTC	1 cut(s)	607
EarI	CTCTTC	1 cut(s)	848
Ecl136II	GAGCTC	1 cut(s)	784
Eco130I	CCWWGG	4 cut(s)	75, 1363, 1839, 1946
Eco24I	GRGCYC	1 cut(s)	786
Eco32I	GATATC	1 cut(s)	450
Eco47I	GGWCC	3 cut(s)	93, 433, 1486
Eco53kI	GAGCTC	1 cut(s)	784
Eco57I	CTGAAG	1 cut(s)	320
Eco72I	CACGTG	1 cut(s)	1814
Eco88I	CYCGRG	1 cut(s)	100
EcoICRI	GAGCTC	1 cut(s)	784
EcoRI	GAATTC	2 cut(s)	634, 1763
EcoRII	CCWGG	2 cut(s)	1261, 2242
EcoRV	GATATC	1 cut(s)	450
EcoT14I	CCWWGG	4 cut(s)	75, 1363, 1839, 1946
EcoT38I	GRGCYC	1 cut(s)	786
ErhI	CCWWGG	4 cut(s)	75, 1363, 1839, 1946
FalI	AAGNNNNNCTT	2 cut(s)	2201, 2233
FaqI	GGGAC	3 cut(s)	494, 1089, 1332
FauI	CCCGC	4 cut(s)	77, 209, 463, 1339
Fnu4HI	GCNGC	8 cut(s)	82, 106, 422, 425, 1011, 1134, 2006, 2079
FokI	GGATG	3 cut(s)	749, 1654, 1979
FriOI	GRGCYC	1 cut(s)	786
Fsp4HI	GCNGC	8 cut(s)	82, 106, 422, 425, 1011, 1134, 2006, 2079
FspBI	CTAG	8 cut(s)	1056, 1187, 1226, 1274, 1358, 1364, 1725, 2064
GlaI	GCGC	5 cut(s)	131, 334, 420, 540, 897
GluI	GCNGC	8 cut(s)	82, 106, 422, 425, 1011, 1134, 2006, 2079
GsuI	CTGGAG	1 cut(s)	1065
HaeII	RGCGCY	1 cut(s)	899
HaeIII	GGCC	4 cut(s)	88, 200, 224, 364
HapII	CCGG	6 cut(s)	101, 234, 267, 395, 909, 1870
HgaI	GACGC	2 cut(s)	14, 97
HhaI	GCGC	5 cut(s)	132, 335, 421, 541, 898
Hin1I	GRCGYC	1 cut(s)	108
Hin6I	GCGC	5 cut(s)	130, 333, 419, 539, 896
HinP1I	GCGC	5 cut(s)	130, 333, 419, 539, 896
HincII	GTYRAC	2 cut(s)	1396, 1501
HindII	GTYRAC	2 cut(s)	1396, 1501
HindIII	AAGCTT	2 cut(s)	44, 2027
HpaII	CCGG	6 cut(s)	101, 234, 267, 395, 909, 1870
HphI	GGTGA	4 cut(s)	617, 781, 991, 1614
Hpy166II	GTNNAC	3 cut(s)	1396, 1501, 1591
Hpy188III	TCNNGA	6 cut(s)	322, 461, 1226, 1829, 1964, 2018
Hpy8I	GTNNAC	3 cut(s)	1396, 1501, 1591
Hpy99I	CGWCG	4 cut(s)	388, 409, 584, 1413
HpyCH4III	ACNGT	7 cut(s)	36, 189, 485, 605, 889, 1400, 1507
HpyCH4IV	ACGT	1 cut(s)	1813
HpyF10VI	GCNNNNNNNGC	8 cut(s)	22, 111, 142, 230, 1034, 1423, 1477, 2087
HpyF3I	CTNAG	6 cut(s)	437, 845, 1536, 1617, 1758, 2025
HpySE526I	ACGT	1 cut(s)	1813
Hsp92I	GRCGYC	1 cut(s)	108
HspAI	GCGC	5 cut(s)	130, 333, 419, 539, 896
KpnI	GGTACC	1 cut(s)	1162
Kzo9I	GATC	6 cut(s)	313, 410, 702, 771, 2014, 2112
LmnI	GCTCC	3 cut(s)	22, 215, 522
Lsp1109I	GCAGC	3 cut(s)	1022, 1120, 1992
LweI	GCATC	4 cut(s)	3, 961, 1761, 2076
MaeI	CTAG	8 cut(s)	1056, 1187, 1226, 1274, 1358, 1364, 1725, 2064
MaeII	ACGT	1 cut(s)	1813
MaeIII	GTNAC	5 cut(s)	1620, 1663, 1792, 1834, 1935
MalI	GATC	6 cut(s)	315, 412, 704, 773, 2016, 2114
MbiI	CCGCTC	1 cut(s)	210
MboI	GATC	6 cut(s)	313, 410, 702, 771, 2014, 2112
MboII	GAAGA	7 cut(s)	350, 567, 712, 865, 1223, 1908, 2233
MfeI	CAATTG	2 cut(s)	2009, 2175
MhlI	GDGCHC	5 cut(s)	260, 273, 723, 786, 1593
MlyI	GAGTC	5 cut(s)	553, 578, 617, 1628, 2180
MmeI	TCCRAC	3 cut(s)	603, 778, 1171
MroXI	GAANNNNTTC	1 cut(s)	1995
MseI	TTAA	6 cut(s)	708, 726, 918, 1542, 1973, 2031
MslI	CAYNNNNRTG	1 cut(s)	1175
MspCI	CTTAAG	1 cut(s)	2030
MspI	CCGG	6 cut(s)	101, 234, 267, 395, 909, 1870
MspR9I	CCNGG	7 cut(s)	101, 102, 268, 910, 1263, 1871, 2244
MunI	CAATTG	2 cut(s)	2009, 2175
MvaI	CCWGG	2 cut(s)	1263, 2244
MvnI	CGCG	1 cut(s)	1201
MwoI	GCNNNNNNNGC	8 cut(s)	22, 111, 142, 230, 1034, 1423, 1477, 2087
NciI	CCSGG	5 cut(s)	101, 102, 268, 910, 1871
NcoI	CCATGG	2 cut(s)	75, 1946
NdeII	GATC	6 cut(s)	313, 410, 702, 771, 2014, 2112
NheI	GCTAGC	1 cut(s)	1055
NlaIV	GGNNCC	2 cut(s)	265, 1160
NmeAIII	GCCGAG	2 cut(s)	515, 1484
NmuCI	GTSAC	2 cut(s)	1620, 1834
NspI	RCATGY	1 cut(s)	136
NspV	TTCGAA	3 cut(s)	626, 638, 1372
PaeI	GCATGC	1 cut(s)	136
PagI	TCATGA	1 cut(s)	321
PaqCI	CACCTGC	1 cut(s)	751
PcsI	WCGNNNNNNNCGW	2 cut(s)	410, 609
PdmI	GAANNNNTTC	1 cut(s)	1995
PfeI	GAWTC	5 cut(s)	596, 1203, 1229, 1786, 2042
PkrI	GCNGC	8 cut(s)	83, 107, 423, 426, 1012, 1135, 2007, 2080
Ple19I	CGATCG	1 cut(s)	316
PleI	GAGTC	5 cut(s)	552, 578, 616, 1627, 2179
PmaCI	CACGTG	1 cut(s)	1814
PmlI	CACGTG	1 cut(s)	1814
PpsI	GAGTC	5 cut(s)	552, 578, 616, 1627, 2179
Ppu21I	YACGTR	1 cut(s)	1814
PshAI	GACNNNNGTC	1 cut(s)	1841
PsiI	TTATAA	2 cut(s)	1632, 2106
Psp124BI	GAGCTC	1 cut(s)	786
Psp6I	CCWGG	2 cut(s)	1261, 2242
PspCI	CACGTG	1 cut(s)	1814
PspGI	CCWGG	2 cut(s)	1261, 2242
PspN4I	GGNNCC	2 cut(s)	265, 1160
PspPI	GGNCC	7 cut(s)	86, 93, 199, 222, 362, 433, 1486
PstI	CTGCAG	3 cut(s)	56, 1012, 1138
PstNI	CAGNNNCTG	2 cut(s)	298, 1600
PvuI	CGATCG	1 cut(s)	316
RsaI	GTAC	5 cut(s)	306, 615, 933, 1160, 1651
RsaNI	GTAC	5 cut(s)	305, 614, 932, 1159, 1650
RseI	CAYNNNNRTG	1 cut(s)	1175
SacI	GAGCTC	1 cut(s)	786
SaqAI	TTAA	6 cut(s)	708, 726, 918, 1542, 1973, 2031
SatI	GCNGC	8 cut(s)	82, 106, 422, 425, 1011, 1134, 2006, 2079
Sau3AI	GATC	6 cut(s)	313, 410, 702, 771, 2014, 2112
Sau96I	GGNCC	7 cut(s)	86, 93, 199, 222, 362, 433, 1486
SchI	GAGTC	5 cut(s)	553, 578, 617, 1628, 2180
ScrFI	CCNGG	7 cut(s)	101, 102, 268, 910, 1263, 1871, 2244
SduI	GDGCHC	5 cut(s)	260, 273, 723, 786, 1593
SfaNI	GCATC	4 cut(s)	3, 961, 1761, 2076
SfcI	CTRYAG	3 cut(s)	52, 1008, 1134
SfuI	TTCGAA	3 cut(s)	626, 638, 1372
SinI	GGWCC	3 cut(s)	93, 433, 1486
SmaI	CCCGGG	1 cut(s)	102
SmiMI	CAYNNNNRTG	1 cut(s)	1175
SmlI	CTYRAG	1 cut(s)	2030
SmoI	CTYRAG	1 cut(s)	2030
SpeI	ACTAGT	1 cut(s)	1186
SphI	GCATGC	1 cut(s)	136
SspMI	CTAG	8 cut(s)	1056, 1187, 1226, 1274, 1358, 1364, 1725, 2064
SstI	GAGCTC	1 cut(s)	786
StyD4I	CCNGG	7 cut(s)	99, 100, 266, 908, 1261, 1869, 2242
StyI	CCWWGG	4 cut(s)	75, 1363, 1839, 1946
TaaI	ACNGT	7 cut(s)	36, 189, 485, 605, 889, 1400, 1507
TaiI	ACGT	1 cut(s)	1816
TatI	WGTACW	1 cut(s)	1649
TauI	GCSGC	5 cut(s)	84, 108, 424, 427, 2081
TfiI	GAWTC	5 cut(s)	596, 1203, 1229, 1786, 2042
Tru1I	TTAA	6 cut(s)	708, 726, 918, 1542, 1973, 2031
Tru9I	TTAA	6 cut(s)	708, 726, 918, 1542, 1973, 2031
TscAI	CASTG	5 cut(s)	265, 435, 490, 738, 1512
TseFI	GTSAC	2 cut(s)	1620, 1834
TseI	GCWGC	3 cut(s)	1010, 1133, 2005
Tsp45I	GTSAC	2 cut(s)	1620, 1834
TspGWI	ACGGA	2 cut(s)	414, 601
TspMI	CCCGGG	1 cut(s)	100
TspRI	CASTG	5 cut(s)	265, 435, 490, 738, 1512
Vha464I	CTTAAG	1 cut(s)	2030
VneI	GTGCAC	1 cut(s)	1589
VpaK11BI	GGWCC	3 cut(s)	93, 433, 1486
XapI	RAATTY	7 cut(s)	216, 490, 634, 641, 1763, 1994, 2162
XbaI	TCTAGA	1 cut(s)	1225
XceI	RCATGY	1 cut(s)	136
XmaI	CCCGGG	1 cut(s)	100
XmaJI	CCTAGG	1 cut(s)	1363
XmnI	GAANNNNTTC	1 cut(s)	1995
XspI	CTAG	8 cut(s)	1056, 1187, 1226, 1274, 1358, 1364, 1725, 2064

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.