AT1G64220

Mitochondrial import receptor subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009204
Physical Location & Seq
Reverse (-)
23833088 .. 23833393
306 bp
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UTR
Exon/CDS
Intron
AT1G64220.1

Sequence Viewer

Length: 234 bp
ATGGCGGCTAAAAGTACGTTGAAGATCAAAGGCAAAGCAAAACCATCAAAGGGATCATCATCATCTTCGTCCTCCTCTGCATCATCAAAGTACAAGGTCTTCAAGGATTGGACCAACTGGTCATTACAGAAAGCCAAAGTCGCGACTCACTATGGTTTCATCCCTCTGATCATCATCATCGGCATGAACTCAGATCCTAAACCTCATCTTTTTCACCTCCTCTCTCCCGTTTAA

Protein Analysis

77

Amino Acids

8.36

Weight (kDa)

10.26

Isoelectric Point (pI)

32.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tom7 PF08038 35 - 74 3.8e-17 TOM7 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 118
AccII CGCG 1 cut(s) 143
AciI CCGC 1 cut(s) 5
AclWI GGATC 2 cut(s) 61, 188
AfaI GTAC 2 cut(s) 16, 92
AfiI CCNNNNNNNGG 1 cut(s) 50
AgsI TTSAA 2 cut(s) 22, 103
AlwI GGATC 2 cut(s) 61, 188
AspS9I GGNCC 1 cut(s) 111
AsuHPI GGTGA 1 cut(s) 206
AvaII GGWCC 1 cut(s) 111
BarI GAAGNNNNNNTAC 2 cut(s) 83, 115
BbsI GAAGAC 1 cut(s) 91
BccI CCATC 1 cut(s) 52
BclI TGATCA 1 cut(s) 168
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme18I GGWCC 1 cut(s) 111
BmgT120I GGNCC 1 cut(s) 111
BmsI GCATC 1 cut(s) 89
BpiI GAAGAC 1 cut(s) 91
BsaBI GATNNNNATC 2 cut(s) 58, 173
Bsc4I CCNNNNNNNGG 1 cut(s) 50
Bse1I ACTGG 1 cut(s) 122
Bse8I GATNNNNATC 2 cut(s) 58, 173
BseGI GGATG 1 cut(s) 159
BseJI GATNNNNATC 2 cut(s) 58, 173
BseLI CCNNNNNNNGG 1 cut(s) 50
BseMII CTCAG 1 cut(s) 204
BseNI ACTGG 1 cut(s) 122
BseRI GAGGAG 2 cut(s) 64, 209
Bsh1236I CGCG 1 cut(s) 143
BslI CCNNNNNNNGG 1 cut(s) 50
Bsp143I GATC 4 cut(s) 24, 53, 168, 193
Bsp68I TCGCGA 1 cut(s) 143
BspACI CCGC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 203
BspFNI CGCG 1 cut(s) 143
BspPI GGATC 2 cut(s) 61, 188
BsrI ACTGG 1 cut(s) 122
BssMI GATC 4 cut(s) 24, 53, 168, 193
BstDEI CTNAG 1 cut(s) 190
BstF5I GGATG 1 cut(s) 159
BstFNI CGCG 1 cut(s) 143
BstKTI GATC 4 cut(s) 27, 56, 171, 196
BstMBI GATC 4 cut(s) 24, 53, 168, 193
BstMWI GCNNNNNNNGC 1 cut(s) 140
BstUI CGCG 1 cut(s) 143
BstV2I GAAGAC 1 cut(s) 91
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
BtsCI GGATG 1 cut(s) 159
BtuMI TCGCGA 1 cut(s) 143
Cfr13I GGNCC 1 cut(s) 111
Csp6I GTAC 2 cut(s) 15, 91
CviAII CATG 1 cut(s) 184
CviJI RGCY 2 cut(s) 8, 134
CviKI_1 RGCY 2 cut(s) 8, 134
CviQI GTAC 2 cut(s) 15, 91
DdeI CTNAG 1 cut(s) 190
DpnI GATC 4 cut(s) 26, 55, 170, 195
DpnII GATC 4 cut(s) 24, 53, 168, 193
DrdI GACNNNNNNGTC 1 cut(s) 118
DseDI GACNNNNNNGTC 1 cut(s) 118
Eco47I GGWCC 1 cut(s) 111
FaeI CATG 1 cut(s) 187
FaiI YATR 2 cut(s) 153, 185
FatI CATG 1 cut(s) 183
FbaI TGATCA 1 cut(s) 168
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 1 cut(s) 146
Fsp4HI GCNGC 1 cut(s) 6
GluI GCNGC 1 cut(s) 6
Hin1II CATG 1 cut(s) 187
HinfI GANTC 1 cut(s) 145
HphI GGTGA 1 cut(s) 206
Hpy188I TCNGA 2 cut(s) 168, 193
Hpy188III TCNNGA 1 cut(s) 142
HpyCH4IV ACGT 1 cut(s) 17
HpyCH4V TGCA 1 cut(s) 80
HpyF10VI GCNNNNNNNGC 1 cut(s) 140
HpyF3I CTNAG 1 cut(s) 190
HpySE526I ACGT 1 cut(s) 17
Hsp92II CATG 1 cut(s) 187
Ksp22I TGATCA 1 cut(s) 168
Kzo9I GATC 4 cut(s) 24, 53, 168, 193
LpnPI CCDG 1 cut(s) 103
LweI GCATC 1 cut(s) 89
MaeII ACGT 1 cut(s) 17
MalI GATC 4 cut(s) 26, 55, 170, 195
MboI GATC 4 cut(s) 24, 53, 168, 193
MboII GAAGA 3 cut(s) 34, 57, 91
MflI RGATCY 1 cut(s) 193
MlyI GAGTC 1 cut(s) 139
MnlI CCTC 6 cut(s) 82, 85, 174, 213, 227, 230
MseI TTAA 1 cut(s) 232
MslI CAYNNNNRTG 1 cut(s) 182
MvnI CGCG 1 cut(s) 143
MwoI GCNNNNNNNGC 1 cut(s) 140
NdeII GATC 4 cut(s) 24, 53, 168, 193
NlaIII CATG 1 cut(s) 187
NruI TCGCGA 1 cut(s) 143
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 1 cut(s) 139
PpsI GAGTC 1 cut(s) 139
PspPI GGNCC 1 cut(s) 111
PsuI RGATCY 1 cut(s) 193
RruI TCGCGA 1 cut(s) 143
RsaI GTAC 2 cut(s) 16, 92
RsaNI GTAC 2 cut(s) 15, 91
RseI CAYNNNNRTG 1 cut(s) 182
SaqAI TTAA 1 cut(s) 232
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 4 cut(s) 24, 53, 168, 193
Sau96I GGNCC 1 cut(s) 111
SchI GAGTC 1 cut(s) 139
SetI ASST 4 cut(s) 20, 99, 205, 219
SfaNI GCATC 1 cut(s) 89
SgeI CNNG 5 cut(s) 106, 115, 130, 154, 196
SinI GGWCC 1 cut(s) 111
SmiMI CAYNNNNRTG 1 cut(s) 182
SsiI CCGC 1 cut(s) 5
TaiI ACGT 1 cut(s) 20
TatI WGTACW 1 cut(s) 90
TauI GCSGC 1 cut(s) 8
Tru1I TTAA 1 cut(s) 232
Tru9I TTAA 1 cut(s) 232
TspDTI ATGAA 2 cut(s) 148, 200
VpaK11BI GGWCC 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.