Rh4DG074800

Mitochondrial import receptor subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0009204
Physical Location & Seq
Reverse (-)
11121643 .. 11122170
528 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG074800.1

Sequence Viewer

Length: 222 bp
ATGGCGTCGAGGGTTAGCTTGAAGAGCAAGGGCAAGACGACTAACAAGGGCTCCAAGGGTTCCGACGACCGGTCAATTTCTCAGGCCGTCAAGGAGTGGACCTCCTGGACCATGAAGAAGGCCAAGGTCGTCACTCACTACGGTTTCATTCCTCTCATCATCGTCATCGGCATGAACTCCGATCCCAAGCCCCAACTTTCTCAGCTTCTCAGCCCCGTCTGA

Protein Analysis

73

Amino Acids

7.99

Weight (kDa)

10.24

Isoelectric Point (pI)

24.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tom7 PF08038 31 - 70 2.3e-19 TOM7 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 176
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 1 cut(s) 69
AgeI ACCGGT 1 cut(s) 69
AgsI TTSAA 1 cut(s) 22
AhdI GACNNNNNGTC 1 cut(s) 70
AjnI CCWGG 1 cut(s) 104
AluBI AGCT 2 cut(s) 18, 205
AluI AGCT 2 cut(s) 18, 205
AlwI GGATC 1 cut(s) 176
AoxI GGCC 2 cut(s) 84, 120
AsiGI ACCGGT 1 cut(s) 69
AspS9I GGNCC 2 cut(s) 99, 108
AvaII GGWCC 2 cut(s) 99, 108
BanII GRGCYC 1 cut(s) 53
BceAI ACGGC 1 cut(s) 71
BciT130I CCWGG 1 cut(s) 106
Bme1390I CCNGG 1 cut(s) 106
Bme18I GGWCC 2 cut(s) 99, 108
BmeRI GACNNNNNGTC 1 cut(s) 70
BmgT120I GGNCC 2 cut(s) 99, 108
BmiI GGNNCC 2 cut(s) 52, 61
BmrFI CCNGG 1 cut(s) 106
BplI GAGNNNNNCTC 2 cut(s) 86, 118
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 54, 123
BsaWI WCCGGW 1 cut(s) 69
BsaXI ACNNNNNCTCC 2 cut(s) 35, 65
Bsc4I CCNNNNNNNGG 1 cut(s) 69
Bse118I RCCGGY 1 cut(s) 69
BseBI CCWGG 1 cut(s) 106
BseDI CCNNGG 2 cut(s) 54, 123
BseLI CCNNNNNNNGG 1 cut(s) 69
BseMII CTCAG 2 cut(s) 95, 215
Bsh1285I CGRYCG 1 cut(s) 70
BshFI GGCC 2 cut(s) 86, 122
BshTI ACCGGT 1 cut(s) 69
BsiEI CGRYCG 1 cut(s) 70
BsiSI CCGG 1 cut(s) 70
BslI CCNNNNNNNGG 1 cut(s) 69
BsnI GGCC 2 cut(s) 86, 122
Bsp1286I GDGCHC 1 cut(s) 53
Bsp143I GATC 1 cut(s) 181
BspANI GGCC 2 cut(s) 86, 122
BspCNI CTCAG 3 cut(s) 94, 214, 222
BspLI GGNNCC 2 cut(s) 52, 61
BspPI GGATC 1 cut(s) 176
BspQI GCTCTTC 1 cut(s) 17
BsrFI RCCGGY 1 cut(s) 69
BssAI RCCGGY 1 cut(s) 69
BssECI CCNNGG 2 cut(s) 54, 123
BssMI GATC 1 cut(s) 181
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 54, 123
Bst2UI CCWGG 1 cut(s) 106
Bst4CI ACNGT 1 cut(s) 143
Bst6I CTCTTC 1 cut(s) 17
BstACI GRCGYC 1 cut(s) 5
BstDEI CTNAG 3 cut(s) 81, 201, 209
BstKTI GATC 1 cut(s) 184
BstMBI GATC 1 cut(s) 181
BstMCI CGRYCG 1 cut(s) 70
BstMWI GCNNNNNNNGC 1 cut(s) 24
BstNI CCWGG 1 cut(s) 106
BstSCI CCNGG 1 cut(s) 104
BsuRI GGCC 2 cut(s) 86, 122
Cfr10I RCCGGY 1 cut(s) 69
Cfr13I GGNCC 2 cut(s) 99, 108
CspAI ACCGGT 1 cut(s) 69
CviAII CATG 2 cut(s) 112, 172
CviJI RGCY 7 cut(s) 18, 51, 86, 122, 190, 205, 213
CviKI_1 RGCY 7 cut(s) 18, 51, 86, 122, 190, 205, 213
DdeI CTNAG 3 cut(s) 81, 201, 209
DpnI GATC 1 cut(s) 183
DpnII GATC 1 cut(s) 181
DriI GACNNNNNGTC 1 cut(s) 70
Eam1104I CTCTTC 1 cut(s) 17
Eam1105I GACNNNNNGTC 1 cut(s) 70
EarI CTCTTC 1 cut(s) 17
Eco130I CCWWGG 2 cut(s) 54, 123
Eco24I GRGCYC 1 cut(s) 53
Eco47I GGWCC 2 cut(s) 99, 108
EcoRII CCWGG 1 cut(s) 104
EcoT14I CCWWGG 2 cut(s) 54, 123
EcoT38I GRGCYC 1 cut(s) 53
ErhI CCWWGG 2 cut(s) 54, 123
FaeI CATG 2 cut(s) 115, 175
FaiI YATR 2 cut(s) 113, 173
FatI CATG 2 cut(s) 111, 171
FriOI GRGCYC 1 cut(s) 53
HaeIII GGCC 2 cut(s) 86, 122
HapII CCGG 1 cut(s) 70
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 115, 175
HpaII CCGG 1 cut(s) 70
Hpy166II GTNNAC 1 cut(s) 99
Hpy188I TCNGA 3 cut(s) 64, 181, 221
Hpy8I GTNNAC 1 cut(s) 99
Hpy99I CGWCG 2 cut(s) 10, 68
HpyAV CCTTC 1 cut(s) 112
HpyCH4III ACNGT 1 cut(s) 143
HpyF10VI GCNNNNNNNGC 1 cut(s) 24
HpyF3I CTNAG 3 cut(s) 81, 201, 209
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 115, 175
Kzo9I GATC 1 cut(s) 181
LguI GCTCTTC 1 cut(s) 17
LmnI GCTCC 1 cut(s) 56
LpnPI CCDG 4 cut(s) 68, 83, 91, 118
MaeIII GTNAC 1 cut(s) 130
MalI GATC 1 cut(s) 183
MboI GATC 1 cut(s) 181
MboII GAAGA 2 cut(s) 34, 127
MhlI GDGCHC 1 cut(s) 53
MluCI AATT 1 cut(s) 75
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 3 cut(s) 3, 112, 162
MslI CAYNNNNRTG 1 cut(s) 170
MspI CCGG 1 cut(s) 70
MspR9I CCNGG 1 cut(s) 106
MvaI CCWGG 1 cut(s) 106
MwoI GCNNNNNNNGC 1 cut(s) 24
NdeII GATC 1 cut(s) 181
NlaIII CATG 2 cut(s) 115, 175
NlaIV GGNNCC 2 cut(s) 52, 61
NmuCI GTSAC 1 cut(s) 130
PciSI GCTCTTC 1 cut(s) 17
PfoI TCCNGGA 1 cut(s) 104
PinAI ACCGGT 1 cut(s) 69
Psp6I CCWGG 1 cut(s) 104
PspGI CCWGG 1 cut(s) 104
PspN4I GGNNCC 2 cut(s) 52, 61
PspPI GGNCC 2 cut(s) 99, 108
RseI CAYNNNNRTG 1 cut(s) 170
SapI GCTCTTC 1 cut(s) 17
Sau3AI GATC 1 cut(s) 181
Sau96I GGNCC 2 cut(s) 99, 108
ScrFI CCNGG 1 cut(s) 106
SduI GDGCHC 1 cut(s) 53
SetI ASST 4 cut(s) 20, 104, 129, 207
SinI GGWCC 2 cut(s) 99, 108
SmiMI CAYNNNNRTG 1 cut(s) 170
Sse9I AATT 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 104
StyI CCWWGG 2 cut(s) 54, 123
TaaI ACNGT 1 cut(s) 143
TaqI TCGA 1 cut(s) 8
TasI AATT 1 cut(s) 75
TseFI GTSAC 1 cut(s) 130
Tsp45I GTSAC 1 cut(s) 130
TspDTI ATGAA 3 cut(s) 128, 136, 188
VpaK11BI GGWCC 2 cut(s) 99, 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.