MD13G1234800.v1.1

Mitochondrial import receptor subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009204
Physical Location & Seq
Reverse (-)
23557073 .. 23557294
222 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1234800.v1.1.491

Sequence Viewer

Length: 222 bp
ATGGCATCGAAGATATCTCTGAAAACCAAGGGCAAGACTCCGGCGAAGGGCTCCAAGGGCTCGGAGGAGCGCTCGGTGGCTCAGTTCGTGAAGGAGTGGAGCACGTGGACCATGAAGAAGGCCAAGGTCGTCACCCACTACGGCTTCATTCCGCTGGTCATCATCATCGGCATGAACTCGGATCCCAAGCCGCAACTTTCCCAGCTGCTCAGTCCCGTCTGA

Protein Analysis

74

Amino Acids

8.02

Weight (kDa)

10.14

Isoelectric Point (pI)

27.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tom7 PF08038 31 - 70 5.7e-19 TOM7 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 152, 191
AclWI GGATC 2 cut(s) 176, 189
AcvI CACGTG 1 cut(s) 105
AfeI AGCGCT 1 cut(s) 71
AfiI CCNNNNNNNGG 1 cut(s) 47
AluBI AGCT 1 cut(s) 205
AluI AGCT 1 cut(s) 205
Alw21I GWGCWC 1 cut(s) 104
AlwI GGATC 2 cut(s) 176, 189
Aor51HI AGCGCT 1 cut(s) 71
AoxI GGCC 1 cut(s) 120
ApeKI GCWGC 1 cut(s) 205
AspLEI GCGC 1 cut(s) 72
AspS9I GGNCC 1 cut(s) 108
AsuHPI GGTGA 1 cut(s) 124
AvaII GGWCC 1 cut(s) 108
BamHI GGATCC 1 cut(s) 181
BanII GRGCYC 2 cut(s) 53, 62
BbrPI CACGTG 1 cut(s) 105
Bbv12I GWGCWC 1 cut(s) 104
BbvI GCAGC 1 cut(s) 192
BceAI ACGGC 1 cut(s) 157
BfoI RGCGCY 1 cut(s) 73
BisI GCNGC 2 cut(s) 191, 206
BlsI GCNGC 2 cut(s) 192, 207
Bme18I GGWCC 1 cut(s) 108
BmgT120I GGNCC 1 cut(s) 108
BmiI GGNNCC 2 cut(s) 52, 183
BmsI GCATC 1 cut(s) 14
BplI GAGNNNNNCTC 2 cut(s) 56, 88
BsaAI YACGTR 1 cut(s) 105
BsaJI CCNNGG 3 cut(s) 27, 54, 123
Bsc4I CCNNNNNNNGG 1 cut(s) 47
BseDI CCNNGG 3 cut(s) 27, 54, 123
BseLI CCNNNNNNNGG 1 cut(s) 47
BseMII CTCAG 1 cut(s) 95
BseRI GAGGAG 1 cut(s) 80
BseXI GCAGC 1 cut(s) 192
BseYI CCCAGC 1 cut(s) 201
BshFI GGCC 1 cut(s) 122
BsiHKAI GWGCWC 1 cut(s) 104
BsiSI CCGG 1 cut(s) 41
BslFI GGGAC 1 cut(s) 198
BslI CCNNNNNNNGG 1 cut(s) 47
BsmFI GGGAC 1 cut(s) 198
BsnI GGCC 1 cut(s) 122
Bsp1286I GDGCHC 3 cut(s) 53, 62, 104
Bsp143I GATC 1 cut(s) 181
BspACI CCGC 2 cut(s) 152, 191
BspANI GGCC 1 cut(s) 122
BspCNI CTCAG 2 cut(s) 94, 222
BspLI GGNNCC 2 cut(s) 52, 183
BspPI GGATC 2 cut(s) 176, 189
BssECI CCNNGG 3 cut(s) 27, 54, 123
BssMI GATC 1 cut(s) 181
BssT1I CCWWGG 3 cut(s) 27, 54, 123
BstBAI YACGTR 1 cut(s) 105
BstDEI CTNAG 2 cut(s) 81, 209
BstH2I RGCGCY 1 cut(s) 73
BstHHI GCGC 1 cut(s) 72
BstKTI GATC 1 cut(s) 184
BstMBI GATC 1 cut(s) 181
BstMWI GCNNNNNNNGC 1 cut(s) 57
BstV1I GCAGC 1 cut(s) 192
BstX2I RGATCY 1 cut(s) 181
BstYI RGATCY 1 cut(s) 181
BsuRI GGCC 1 cut(s) 122
CfoI GCGC 1 cut(s) 72
Cfr13I GGNCC 1 cut(s) 108
CviAII CATG 2 cut(s) 112, 172
CviJI RGCY 7 cut(s) 51, 60, 80, 122, 144, 190, 205
CviKI_1 RGCY 7 cut(s) 51, 60, 80, 122, 144, 190, 205
DdeI CTNAG 2 cut(s) 81, 209
DpnI GATC 1 cut(s) 183
DpnII GATC 1 cut(s) 181
Eco130I CCWWGG 3 cut(s) 27, 54, 123
Eco24I GRGCYC 2 cut(s) 53, 62
Eco32I GATATC 1 cut(s) 15
Eco47I GGWCC 1 cut(s) 108
Eco47III AGCGCT 1 cut(s) 71
Eco72I CACGTG 1 cut(s) 105
EcoRV GATATC 1 cut(s) 15
EcoT14I CCWWGG 3 cut(s) 27, 54, 123
EcoT38I GRGCYC 2 cut(s) 53, 62
ErhI CCWWGG 3 cut(s) 27, 54, 123
FaeI CATG 2 cut(s) 115, 175
FaiI YATR 2 cut(s) 113, 173
FaqI GGGAC 1 cut(s) 198
FatI CATG 2 cut(s) 111, 171
Fnu4HI GCNGC 2 cut(s) 191, 206
FriOI GRGCYC 2 cut(s) 53, 62
Fsp4HI GCNGC 2 cut(s) 191, 206
GlaI GCGC 1 cut(s) 71
GluI GCNGC 2 cut(s) 191, 206
GsaI CCCAGC 1 cut(s) 205
HaeII RGCGCY 1 cut(s) 73
HaeIII GGCC 1 cut(s) 122
HapII CCGG 1 cut(s) 41
HhaI GCGC 1 cut(s) 72
Hin1II CATG 2 cut(s) 115, 175
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HinfI GANTC 1 cut(s) 37
HpaII CCGG 1 cut(s) 41
HphI GGTGA 1 cut(s) 124
Hpy166II GTNNAC 1 cut(s) 108
Hpy188I TCNGA 4 cut(s) 21, 64, 181, 221
Hpy188III TCNNGA 1 cut(s) 88
Hpy8I GTNNAC 1 cut(s) 108
HpyAV CCTTC 3 cut(s) 40, 85, 112
HpyCH4IV ACGT 1 cut(s) 104
HpyF10VI GCNNNNNNNGC 1 cut(s) 57
HpyF3I CTNAG 2 cut(s) 81, 209
HpySE526I ACGT 1 cut(s) 104
Hsp92II CATG 2 cut(s) 115, 175
HspAI GCGC 1 cut(s) 70
Kzo9I GATC 1 cut(s) 181
LmnI GCTCC 3 cut(s) 56, 67, 99
LpnPI CCDG 3 cut(s) 54, 140, 215
Lsp1109I GCAGC 1 cut(s) 192
LweI GCATC 1 cut(s) 14
MaeII ACGT 1 cut(s) 104
MaeIII GTNAC 1 cut(s) 130
MalI GATC 1 cut(s) 183
MboI GATC 1 cut(s) 181
MboII GAAGA 2 cut(s) 22, 127
MflI RGATCY 1 cut(s) 181
MhlI GDGCHC 3 cut(s) 53, 62, 104
MlyI GAGTC 1 cut(s) 31
MnlI CCTC 1 cut(s) 58
MslI CAYNNNNRTG 1 cut(s) 170
MspA1I CMGCKG 2 cut(s) 154, 205
MspI CCGG 1 cut(s) 41
MwoI GCNNNNNNNGC 1 cut(s) 57
NdeII GATC 1 cut(s) 181
NlaIII CATG 2 cut(s) 115, 175
NlaIV GGNNCC 2 cut(s) 52, 183
NmuCI GTSAC 1 cut(s) 130
PkrI GCNGC 2 cut(s) 192, 207
PleI GAGTC 1 cut(s) 31
PmaCI CACGTG 1 cut(s) 105
PmlI CACGTG 1 cut(s) 105
PpsI GAGTC 1 cut(s) 31
Ppu21I YACGTR 1 cut(s) 105
PspCI CACGTG 1 cut(s) 105
PspFI CCCAGC 1 cut(s) 201
PspN4I GGNNCC 2 cut(s) 52, 183
PspPI GGNCC 1 cut(s) 108
PsuI RGATCY 1 cut(s) 181
PvuII CAGCTG 1 cut(s) 205
RseI CAYNNNNRTG 1 cut(s) 170
SatI GCNGC 2 cut(s) 191, 206
Sau3AI GATC 1 cut(s) 181
Sau96I GGNCC 1 cut(s) 108
SchI GAGTC 1 cut(s) 31
SduI GDGCHC 3 cut(s) 53, 62, 104
SetI ASST 3 cut(s) 107, 129, 207
SfaNI GCATC 1 cut(s) 14
SinI GGWCC 1 cut(s) 108
SmiMI CAYNNNNRTG 1 cut(s) 170
SsiI CCGC 2 cut(s) 152, 191
StyI CCWWGG 3 cut(s) 27, 54, 123
TaiI ACGT 1 cut(s) 107
TaqI TCGA 1 cut(s) 8
TauI GCSGC 1 cut(s) 193
TseFI GTSAC 1 cut(s) 130
TseI GCWGC 1 cut(s) 205
Tsp45I GTSAC 1 cut(s) 130
TspDTI ATGAA 3 cut(s) 128, 136, 188
VpaK11BI GGWCC 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.