MD16G1239600.v1.1

Mitochondrial import receptor subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009204
Physical Location & Seq
Reverse (-)
25410314 .. 25410535
222 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1239600.v1.1.491

Sequence Viewer

Length: 222 bp
ATGGCGTCGAGAATATCTCTGAAGAGCAAGGGCAAGACTCCGGCGAAGCCCTCTAAGGGCTCGGAGGAGCGCTCGGTGGCTCAATCCTTCAAGGAGTGGAGCACGTGGGCCCTGAAGAAGGCCAAGGTCGTCACCCACTACGGCTTCATTCCGCTGGTCATCATCATCGGCATGAACTCGGAACCCAAGCCGCAACTTTCCCAACTTCTCAGCCCCGTCTGA

Protein Analysis

74

Amino Acids

8.02

Weight (kDa)

10.24

Isoelectric Point (pI)

43.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tom7 PF08038 31 - 70 7.4e-19 TOM7 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 152, 191
AcuI CTGAAG 2 cut(s) 41, 134
AcvI CACGTG 1 cut(s) 105
AcyI GRCGYC 1 cut(s) 5
AfeI AGCGCT 1 cut(s) 71
AfiI CCNNNNNNNGG 2 cut(s) 56, 118
AgsI TTSAA 1 cut(s) 91
Alw21I GWGCWC 1 cut(s) 104
Aor51HI AGCGCT 1 cut(s) 71
AoxI GGCC 2 cut(s) 108, 120
ApaI GGGCCC 1 cut(s) 112
AspLEI GCGC 1 cut(s) 72
AspS9I GGNCC 2 cut(s) 108, 109
AsuHPI GGTGA 1 cut(s) 124
BaeGI GKGCMC 1 cut(s) 112
BanII GRGCYC 2 cut(s) 62, 112
BbrPI CACGTG 1 cut(s) 105
Bbv12I GWGCWC 1 cut(s) 104
BceAI ACGGC 1 cut(s) 157
BfoI RGCGCY 1 cut(s) 73
BisI GCNGC 1 cut(s) 191
BlsI GCNGC 1 cut(s) 192
BmgT120I GGNCC 2 cut(s) 108, 109
BmiI GGNNCC 2 cut(s) 110, 183
BplI GAGNNNNNCTC 3 cut(s) 33, 56, 88
BsaAI YACGTR 1 cut(s) 105
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 123
Bsc4I CCNNNNNNNGG 2 cut(s) 56, 118
BseDI CCNNGG 1 cut(s) 123
BseLI CCNNNNNNNGG 2 cut(s) 56, 118
BseRI GAGGAG 1 cut(s) 80
BseSI GKGCMC 1 cut(s) 112
BshFI GGCC 2 cut(s) 110, 122
BsiHKAI GWGCWC 1 cut(s) 104
BsiSI CCGG 1 cut(s) 41
BslI CCNNNNNNNGG 2 cut(s) 56, 118
BsnI GGCC 2 cut(s) 110, 122
Bsp120I GGGCCC 1 cut(s) 108
Bsp1286I GDGCHC 3 cut(s) 62, 104, 112
BspACI CCGC 2 cut(s) 152, 191
BspANI GGCC 2 cut(s) 110, 122
BspCNI CTCAG 1 cut(s) 222
BspLI GGNNCC 2 cut(s) 110, 183
BspQI GCTCTTC 1 cut(s) 17
BssECI CCNNGG 1 cut(s) 123
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 123
Bst6I CTCTTC 1 cut(s) 17
BstACI GRCGYC 1 cut(s) 5
BstBAI YACGTR 1 cut(s) 105
BstDEI CTNAG 2 cut(s) 54, 209
BstENI CCTNNNNNAGG 1 cut(s) 116
BstH2I RGCGCY 1 cut(s) 73
BstHHI GCGC 1 cut(s) 72
BstSLI GKGCMC 1 cut(s) 112
BsuRI GGCC 2 cut(s) 110, 122
CfoI GCGC 1 cut(s) 72
Cfr13I GGNCC 2 cut(s) 108, 109
CviAII CATG 1 cut(s) 172
CviJI RGCY 8 cut(s) 49, 60, 80, 110, 122, 144, 190, 213
CviKI_1 RGCY 8 cut(s) 49, 60, 80, 110, 122, 144, 190, 213
DdeI CTNAG 2 cut(s) 54, 209
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
Eco130I CCWWGG 1 cut(s) 123
Eco24I GRGCYC 2 cut(s) 62, 112
Eco47III AGCGCT 1 cut(s) 71
Eco57I CTGAAG 2 cut(s) 41, 134
Eco72I CACGTG 1 cut(s) 105
EcoNI CCTNNNNNAGG 1 cut(s) 116
EcoO109I RGGNCCY 1 cut(s) 109
EcoT14I CCWWGG 1 cut(s) 123
EcoT38I GRGCYC 2 cut(s) 62, 112
ErhI CCWWGG 1 cut(s) 123
FaeI CATG 1 cut(s) 175
FaiI YATR 1 cut(s) 173
FatI CATG 1 cut(s) 171
Fnu4HI GCNGC 1 cut(s) 191
FriOI GRGCYC 2 cut(s) 62, 112
Fsp4HI GCNGC 1 cut(s) 191
GlaI GCGC 1 cut(s) 71
GluI GCNGC 1 cut(s) 191
HaeII RGCGCY 1 cut(s) 73
HaeIII GGCC 2 cut(s) 110, 122
HapII CCGG 1 cut(s) 41
HhaI GCGC 1 cut(s) 72
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 1 cut(s) 175
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HinfI GANTC 1 cut(s) 37
HpaII CCGG 1 cut(s) 41
HphI GGTGA 1 cut(s) 124
Hpy188I TCNGA 4 cut(s) 21, 64, 181, 221
Hpy188III TCNNGA 1 cut(s) 9
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 2 cut(s) 97, 112
HpyCH4IV ACGT 1 cut(s) 104
HpyF3I CTNAG 2 cut(s) 54, 209
HpySE526I ACGT 1 cut(s) 104
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 1 cut(s) 175
HspAI GCGC 1 cut(s) 70
LguI GCTCTTC 1 cut(s) 17
LmnI GCTCC 2 cut(s) 67, 99
LpnPI CCDG 3 cut(s) 54, 125, 140
MaeII ACGT 1 cut(s) 104
MaeIII GTNAC 1 cut(s) 130
MboII GAAGA 2 cut(s) 34, 127
MhlI GDGCHC 3 cut(s) 62, 104, 112
MlyI GAGTC 1 cut(s) 31
MnlI CCTC 2 cut(s) 58, 61
MslI CAYNNNNRTG 1 cut(s) 170
MspA1I CMGCKG 1 cut(s) 154
MspI CCGG 1 cut(s) 41
NlaIII CATG 1 cut(s) 175
NlaIV GGNNCC 2 cut(s) 110, 183
NmuCI GTSAC 1 cut(s) 130
PciSI GCTCTTC 1 cut(s) 17
PkrI GCNGC 1 cut(s) 192
PleI GAGTC 1 cut(s) 31
PmaCI CACGTG 1 cut(s) 105
PmlI CACGTG 1 cut(s) 105
PpsI GAGTC 1 cut(s) 31
Ppu21I YACGTR 1 cut(s) 105
PspCI CACGTG 1 cut(s) 105
PspN4I GGNNCC 2 cut(s) 110, 183
PspOMI GGGCCC 1 cut(s) 108
PspPI GGNCC 2 cut(s) 108, 109
RseI CAYNNNNRTG 1 cut(s) 170
SapI GCTCTTC 1 cut(s) 17
SatI GCNGC 1 cut(s) 191
Sau96I GGNCC 2 cut(s) 108, 109
SchI GAGTC 1 cut(s) 31
SduI GDGCHC 3 cut(s) 62, 104, 112
SetI ASST 2 cut(s) 107, 129
SmiMI CAYNNNNRTG 1 cut(s) 170
SsiI CCGC 2 cut(s) 152, 191
StyI CCWWGG 1 cut(s) 123
TaiI ACGT 1 cut(s) 107
TaqI TCGA 1 cut(s) 8
TauI GCSGC 1 cut(s) 193
TseFI GTSAC 1 cut(s) 130
Tsp45I GTSAC 1 cut(s) 130
TspDTI ATGAA 2 cut(s) 136, 188
XagI CCTNNNNNAGG 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.