Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005226

Physical Location & Seq

Genomic Coordinates Forward (+)

24538886 .. 24542712

Gene Structure 3827 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G65930.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1233 bp

ATGGCGTTTGAGAAGATCAAGGTGGCAAATCCCATCGTCGAGATGGATGGTGATGAAATGACCAGGGTTATTTGGAAGTCTATCAAGGATAAGCTTATTACTCCTTTTGTGGAGTTGGATATCAAGTACTTCGACCTTGGTCTTCCTCACCGTGATGCTACGGATGACAAAGTTACTATTGAAAGTGCTGAAGCTACTAAGAAGTACAATGTGGCGATCAAGTGTGCCACCATCACTCCAGATGAAGGCCGTGTCACGGAATTTGGCTTGAAGCAGATGTGGAGAAGTCCAAATGGGACCATCAGGAATATCCTGAATGGAACTGTATTTAGAGAGCCAATTATCTGCAAAAATGTTCCCAAGCTTGTCCCAGGCTGGACAAAGCCCATCTGCATTGGAAGGCATGCTTTTGGTGATCAGTACCGTGCCACTGATGCTGTAATCAAGGGACCAGGAAAACTGACTATGACCTTTGAGGGAAAAGATGGGAAAACTGAAACCGAAGTCTTTACATTTACTGGTGAAGGTGGTGTTGCTATGGCAATGTACAACACTGATGAGTCCATCCGTGCTTTTGCTGATGCATCGATGAACACTGCTTATGAGAAAAAGTGGCCACTTTACCTTAGCACAAAGAATACCATTCTTAAGAAATACGATGGCAGATTCAAAGACATCTTCCAAGAAGTCTATGAAGCCAGCTGGAAGTCGAAGTATGATGCTGCTGGAATCTGGTATGAACACCGTCTCATTGATGATATGGTGGCCTACGCTCTTAAGAGTGAGGGAGGCTATGTTTGGGCATGCAAAAACTATGATGGTGATGTCCAAAGTGATTTCTTGGCACAAGGATTCGGGTCACTTGGATTGATGACATCTGTTCTGGTCTGCCCAGATGGAAAGACGATTGAAGCTGAAGCGGCCCATGGAACTGTCACCCGTCACTTCAGGGTTCATCAGAAAGGTGGTGAGACCAGCACAAACAGCATAGCCTCTATTTTTGCTTGGACTCGTGGACTTGCACACAGGGCTAAGTTAGATGACAACGCAAAACTCTTGGATTTCACTGAGAAGCTGGAAGCCGCTTGTGTTGGGACAGTGGAGTCAGGGAAAATGACCAAAGATCTTGCACTCATCATTCACGGCTCAAAGCTTTCGAGGGACACGTACCTGAACACAGAGGAGTTCATCGATGCAGTCGCTGCTGAGCTCAAAGAAAGACTCAATGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004448 GO:0004450 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006102 GO:0006139 GO:0006725 GO:0006732 GO:0006733 GO:0006739 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009117 GO:0009506 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009651 GO:0009987 GO:0010035 GO:0010038 GO:0010043 GO:0016020 GO:0016491 GO:0016614 GO:0016616 GO:0019362 GO:0019637 GO:0019752 GO:0030054 GO:0034641 GO:0042221 GO:0042742 GO:0043167 GO:0043169 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046496 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050896 GO:0051186 GO:0051704 GO:0051707 GO:0055044 GO:0055086 GO:0055114 GO:0071704 GO:0071944 GO:0072350 GO:0072524 GO:0098542 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00020 ko00480 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 ko04146 map00020 map00480 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230 map04146

Pfam Domains

Protein Families

Iso_dh

Protein Analysis

410

Amino Acids

45.75

Weight (kDa)

6.13

Isoelectric Point (pI)

19.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Iso_dh	PF00180	7 - 400	1.4e-83	Isocitrate/isopropylmalate dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005226)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65930
fragaria_vesca	FvH4_1g27490 FvH4_1g27490 FvH4_1g30530 FvH4_1g30530
malus_domestica	MD09G1276900.v1.1 MD09G1277300.v1.1 MD17G1270700.v1.1
prunus_persica	Prupe.3G015500_v2.0.a1 Prupe.3G015500_v2.0.a1 Prupe.3G015500_v2.0.a1 Prupe.3G015500_v2.0.a1
pyrus_communis	pycom09g18670 pycom17g26910
rosa_chinensis	RchiOBHm_Chr3g0489041 RchiOBHm_Chr3g0489051
rosa_laevigata	RLG00000022897 RLG00000022898
rosa_multiflora	Rmu_sc0002227.1_g000003 Rmu_sc0011911.1_g000008
rosa_roxburghii	Rroxscaffold_6G00393730
rosa_rugosa	Rorug03G0242800 Rorug03G0242900 Rorug03G0242900
rosa_samantha	Rh3AG291100 Rh3BG327800 Rh3DG324200
rosa_wichuraiana	Rw3G025760 Rw3G025770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1102
AciI	CCGC	2 cut(s)	920, 1083
AcoI	YGGCCR	1 cut(s)	614
AcsI	RAATTY	1 cut(s)	260
AcuI	CTGAAG	3 cut(s)	210, 931, 936
AfaI	GTAC	5 cut(s)	128, 206, 422, 548, 1169
AfiI	CCNNNNNNNGG	2 cut(s)	245, 256
AflII	CTTAAG	2 cut(s)	647, 776
AflIII	ACRYGT	1 cut(s)	1164
AgsI	TTSAA	4 cut(s)	182, 271, 670, 911
AjnI	CCWGG	3 cut(s)	62, 370, 451
AluBI	AGCT	8 cut(s)	94, 194, 364, 702, 914, 1075, 1153, 1210
AluI	AGCT	8 cut(s)	94, 194, 364, 702, 914, 1075, 1153, 1210
Alw21I	GWGCWC	1 cut(s)	1212
Alw26I	GTCTC	2 cut(s)	752, 965
AlwNI	CAGNNNCTG	1 cut(s)	1202
AoxI	GGCC	4 cut(s)	247, 614, 765, 921
ApeKI	GCWGC	2 cut(s)	722, 1202
ApoI	RAATTY	1 cut(s)	260
AspS9I	GGNCC	3 cut(s)	297, 449, 922
AsuHPI	GGTGA	7 cut(s)	62, 140, 425, 533, 833, 928, 980
AvaII	GGWCC	2 cut(s)	297, 449
BalI	TGGCCA	1 cut(s)	616
BanII	GRGCYC	1 cut(s)	1212
BauI	CACGAG	1 cut(s)	1011
BbsI	GAAGAC	1 cut(s)	134
Bbv12I	GWGCWC	1 cut(s)	1212
BbvI	GCAGC	2 cut(s)	709, 1189
BceAI	ACGGC	2 cut(s)	234, 1159
BciT130I	CCWGG	3 cut(s)	64, 372, 453
BclI	TGATCA	1 cut(s)	415
BcoDI	GTCTC	2 cut(s)	752, 965
BfrI	CTTAAG	2 cut(s)	647, 776
BglII	AGATCT	1 cut(s)	1123
BisI	GCNGC	4 cut(s)	723, 921, 1083, 1203
BlpI	GCTNAGC	1 cut(s)	1206
BlsI	GCNGC	4 cut(s)	724, 922, 1084, 1204
BmcAI	AGTACT	1 cut(s)	128
Bme1390I	CCNGG	3 cut(s)	64, 372, 453
Bme18I	GGWCC	2 cut(s)	297, 449
BmgT120I	GGNCC	3 cut(s)	297, 449, 922
BmiI	GGNNCC	2 cut(s)	298, 450
BmrFI	CCNGG	3 cut(s)	64, 372, 453
BmsI	GCATC	6 cut(s)	145, 424, 571, 593, 709, 1183
BoxI	GACNNNNGTC	1 cut(s)	138
BpiI	GAAGAC	1 cut(s)	134
BpmI	CTGGAG	1 cut(s)	222
Bpu10I	CCTNAGC	1 cut(s)	626
Bpu1102I	GCTNAGC	1 cut(s)	1206
Bsa29I	ATCGAT	2 cut(s)	587, 1191
BsaAI	YACGTR	1 cut(s)	1167
BsaI	GGTCTC	1 cut(s)	965
BsaJI	CCNNGG	4 cut(s)	63, 136, 370, 925
BsaXI	ACNNNNNCTCC	4 cut(s)	220, 250, 780, 810
Bsc4I	CCNNNNNNNGG	2 cut(s)	245, 256
Bse1I	ACTGG	1 cut(s)	523
Bse3DI	GCAATG	1 cut(s)	549
BseBI	CCWGG	3 cut(s)	64, 372, 453
BseCI	ATCGAT	2 cut(s)	587, 1191
BseDI	CCNNGG	4 cut(s)	63, 136, 370, 925
BseGI	GGATG	3 cut(s)	52, 169, 564
BseLI	CCNNNNNNNGG	2 cut(s)	245, 256
BseMI	GCAATG	1 cut(s)	549
BseMII	CTCAG	2 cut(s)	1059, 1197
BseNI	ACTGG	1 cut(s)	523
BseRI	GAGGAG	1 cut(s)	1196
BseXI	GCAGC	2 cut(s)	709, 1189
BshFI	GGCC	4 cut(s)	249, 616, 767, 923
BshVI	ATCGAT	2 cut(s)	587, 1191
BsiHKAI	GWGCWC	1 cut(s)	1212
BslFI	GGGAC	5 cut(s)	310, 353, 462, 1108, 1175
BslI	CCNNNNNNNGG	2 cut(s)	245, 256
BsmAI	GTCTC	2 cut(s)	752, 965
BsmBI	CGTCTC	1 cut(s)	752
BsmFI	GGGAC	5 cut(s)	310, 353, 462, 1108, 1175
BsnI	GGCC	4 cut(s)	249, 616, 767, 923
Bso31I	GGTCTC	1 cut(s)	965
Bsp1286I	GDGCHC	1 cut(s)	1212
Bsp1407I	TGTACA	1 cut(s)	546
Bsp143I	GATC	4 cut(s)	15, 216, 415, 1123
Bsp1720I	GCTNAGC	1 cut(s)	1206
Bsp19I	CCATGG	1 cut(s)	925
BspACI	CCGC	2 cut(s)	920, 1083
BspANI	GGCC	4 cut(s)	249, 616, 767, 923
BspCNI	CTCAG	2 cut(s)	1060, 1198
BspDI	ATCGAT	2 cut(s)	587, 1191
BspLI	GGNNCC	2 cut(s)	298, 450
BspTI	CTTAAG	2 cut(s)	647, 776
BspTNI	GGTCTC	1 cut(s)	965
BsrDI	GCAATG	1 cut(s)	549
BsrGI	TGTACA	1 cut(s)	546
BsrI	ACTGG	1 cut(s)	523
BssECI	CCNNGG	4 cut(s)	63, 136, 370, 925
BssMI	GATC	4 cut(s)	15, 216, 415, 1123
BssSI	CACGAG	1 cut(s)	1011
BssT1I	CCWWGG	2 cut(s)	136, 925
Bst2BI	CACGAG	1 cut(s)	1011
Bst2UI	CCWGG	3 cut(s)	64, 372, 453
Bst4CI	ACNGT	6 cut(s)	152, 325, 425, 746, 934, 1099
BstAFI	CTTAAG	2 cut(s)	647, 776
BstAPI	GCANNNNNTGC	1 cut(s)	1202
BstAUI	TGTACA	1 cut(s)	546
BstBAI	YACGTR	1 cut(s)	1167
BstC8I	GCNNGC	3 cut(s)	405, 700, 805
BstDEI	CTNAG	5 cut(s)	198, 626, 1032, 1068, 1206
BstDSI	CCRYGG	1 cut(s)	925
BstF5I	GGATG	3 cut(s)	52, 169, 564
BstKTI	GATC	4 cut(s)	18, 219, 418, 1126
BstMAI	GTCTC	2 cut(s)	752, 965
BstMBI	GATC	4 cut(s)	15, 216, 415, 1123
BstMWI	GCNNNNNNNGC	5 cut(s)	434, 920, 984, 1028, 1202
BstNI	CCWGG	3 cut(s)	64, 372, 453
BstNSI	RCATGY	2 cut(s)	407, 807
BstPAI	GACNNNNGTC	1 cut(s)	138
BstSCI	CCNGG	3 cut(s)	62, 370, 451
BstV1I	GCAGC	2 cut(s)	709, 1189
BstV2I	GAAGAC	1 cut(s)	134
BstX2I	RGATCY	1 cut(s)	1123
BstYI	RGATCY	1 cut(s)	1123
Bsu15I	ATCGAT	2 cut(s)	587, 1191
BsuRI	GGCC	4 cut(s)	249, 616, 767, 923
BsuTUI	ATCGAT	2 cut(s)	587, 1191
BtgI	CCRYGG	1 cut(s)	925
BtsCI	GGATG	3 cut(s)	52, 169, 564
BtsI	GCAGTG	1 cut(s)	594
BtsIMutI	CAGTG	5 cut(s)	429, 552, 594, 1065, 1104
Cac8I	GCNNGC	3 cut(s)	405, 700, 805
CaiI	CAGNNNCTG	1 cut(s)	1202
Cfr13I	GGNCC	3 cut(s)	297, 449, 922
ClaI	ATCGAT	2 cut(s)	587, 1191
Csp6I	GTAC	5 cut(s)	127, 205, 421, 547, 1168
CviAII	CATG	3 cut(s)	404, 804, 926
CviQI	GTAC	5 cut(s)	127, 205, 421, 547, 1168
DdeI	CTNAG	5 cut(s)	198, 626, 1032, 1068, 1206
DpnI	GATC	4 cut(s)	17, 218, 417, 1125
DpnII	GATC	4 cut(s)	15, 216, 415, 1123
DrdI	GACNNNNNNGTC	1 cut(s)	1102
DseDI	GACNNNNNNGTC	1 cut(s)	1102
EaeI	YGGCCR	1 cut(s)	614
Ecl136II	GAGCTC	1 cut(s)	1210
Eco130I	CCWWGG	2 cut(s)	136, 925
Eco24I	GRGCYC	1 cut(s)	1212
Eco31I	GGTCTC	1 cut(s)	965
Eco32I	GATATC	1 cut(s)	121
Eco47I	GGWCC	2 cut(s)	297, 449
Eco53kI	GAGCTC	1 cut(s)	1210
Eco57I	CTGAAG	3 cut(s)	210, 931, 936
EcoICRI	GAGCTC	1 cut(s)	1210
EcoRII	CCWGG	3 cut(s)	62, 370, 451
EcoRV	GATATC	1 cut(s)	121
EcoT14I	CCWWGG	2 cut(s)	136, 925
EcoT22I	ATGCAT	1 cut(s)	586
EcoT38I	GRGCYC	1 cut(s)	1212
ErhI	CCWWGG	2 cut(s)	136, 925
Esp3I	CGTCTC	1 cut(s)	752
FaeI	CATG	3 cut(s)	407, 807, 929
FalI	AAGNNNNNCTT	2 cut(s)	391, 423
FaqI	GGGAC	5 cut(s)	310, 353, 462, 1108, 1175
FatI	CATG	3 cut(s)	403, 803, 925
FbaI	TGATCA	1 cut(s)	415
Fnu4HI	GCNGC	4 cut(s)	723, 921, 1083, 1203
FokI	GGATG	3 cut(s)	59, 176, 551
FriOI	GRGCYC	1 cut(s)	1212
Fsp4HI	GCNGC	4 cut(s)	723, 921, 1083, 1203
GluI	GCNGC	4 cut(s)	723, 921, 1083, 1203
GsuI	CTGGAG	1 cut(s)	222
HaeIII	GGCC	4 cut(s)	249, 616, 767, 923
Hin1II	CATG	3 cut(s)	407, 807, 929
HindIII	AAGCTT	3 cut(s)	92, 362, 1151
HinfI	GANTC	7 cut(s)	560, 666, 729, 852, 1009, 1103, 1221
HphI	GGTGA	7 cut(s)	62, 140, 425, 533, 833, 928, 980
Hpy166II	GTNNAC	1 cut(s)	1016
Hpy188I	TCNGA	1 cut(s)	960
Hpy188III	TCNNGA	4 cut(s)	40, 239, 304, 313
Hpy8I	GTNNAC	1 cut(s)	1016
Hpy99I	CGWCG	1 cut(s)	41
HpyAV	CCTTC	3 cut(s)	239, 393, 518
HpyCH4III	ACNGT	6 cut(s)	152, 325, 425, 746, 934, 1099
HpyCH4IV	ACGT	1 cut(s)	1166
HpyCH4V	TGCA	7 cut(s)	348, 393, 584, 807, 1022, 1130, 1196
HpyF10VI	GCNNNNNNNGC	5 cut(s)	434, 920, 984, 1028, 1202
HpyF3I	CTNAG	5 cut(s)	198, 626, 1032, 1068, 1206
HpySE526I	ACGT	1 cut(s)	1166
Hsp92II	CATG	3 cut(s)	407, 807, 929
Ksp22I	TGATCA	1 cut(s)	415
Kzo9I	GATC	4 cut(s)	15, 216, 415, 1123
Lsp1109I	GCAGC	2 cut(s)	709, 1189
LweI	GCATC	6 cut(s)	145, 424, 571, 593, 709, 1183
MaeII	ACGT	1 cut(s)	1166
MaeIII	GTNAC	5 cut(s)	172, 253, 858, 934, 941
MalI	GATC	4 cut(s)	17, 218, 417, 1125
MboI	GATC	4 cut(s)	15, 216, 415, 1123
MboII	GAAGA	3 cut(s)	25, 134, 670
MflI	RGATCY	1 cut(s)	1123
MhlI	GDGCHC	1 cut(s)	1212
MlsI	TGGCCA	1 cut(s)	616
MluCI	AATT	2 cut(s)	260, 339
MluNI	TGGCCA	1 cut(s)	616
MlyI	GAGTC	4 cut(s)	569, 1003, 1112, 1215
MmeI	TCCRAC	1 cut(s)	96
MnlI	CCTC	7 cut(s)	156, 469, 778, 782, 1003, 1152, 1174
Mox20I	TGGCCA	1 cut(s)	616
Mph1103I	ATGCAT	1 cut(s)	586
MscI	TGGCCA	1 cut(s)	616
MseI	TTAA	3 cut(s)	648, 777, 1231
MslI	CAYNNNNRTG	1 cut(s)	153
Msp20I	TGGCCA	1 cut(s)	616
MspA1I	CMGCKG	1 cut(s)	702
MspCI	CTTAAG	2 cut(s)	647, 776
MspR9I	CCNGG	3 cut(s)	64, 372, 453
MvaI	CCWGG	3 cut(s)	64, 372, 453
MwoI	GCNNNNNNNGC	5 cut(s)	434, 920, 984, 1028, 1202
NcoI	CCATGG	1 cut(s)	925
NdeII	GATC	4 cut(s)	15, 216, 415, 1123
NlaIII	CATG	3 cut(s)	407, 807, 929
NlaIV	GGNNCC	2 cut(s)	298, 450
NmuCI	GTSAC	4 cut(s)	253, 858, 934, 941
NsiI	ATGCAT	1 cut(s)	586
NspI	RCATGY	2 cut(s)	407, 807
PaeI	GCATGC	2 cut(s)	407, 807
PcsI	WCGNNNNNNNCGW	1 cut(s)	1163
PfeI	GAWTC	3 cut(s)	666, 729, 852
PkrI	GCNGC	4 cut(s)	724, 922, 1084, 1204
PleI	GAGTC	4 cut(s)	568, 1003, 1111, 1215
PpsI	GAGTC	4 cut(s)	568, 1003, 1111, 1215
Ppu21I	YACGTR	1 cut(s)	1167
PshAI	GACNNNNGTC	1 cut(s)	138
Psp124BI	GAGCTC	1 cut(s)	1212
Psp6I	CCWGG	3 cut(s)	62, 370, 451
PspGI	CCWGG	3 cut(s)	62, 370, 451
PspN4I	GGNNCC	2 cut(s)	298, 450
PspPI	GGNCC	3 cut(s)	297, 449, 922
PstNI	CAGNNNCTG	1 cut(s)	1202
PsuI	RGATCY	1 cut(s)	1123
PvuII	CAGCTG	1 cut(s)	702
RsaI	GTAC	5 cut(s)	128, 206, 422, 548, 1169
RsaNI	GTAC	5 cut(s)	127, 205, 421, 547, 1168
RseI	CAYNNNNRTG	1 cut(s)	153
SacI	GAGCTC	1 cut(s)	1212
SaqAI	TTAA	3 cut(s)	648, 777, 1231
SatI	GCNGC	4 cut(s)	723, 921, 1083, 1203
Sau3AI	GATC	4 cut(s)	15, 216, 415, 1123
Sau96I	GGNCC	3 cut(s)	297, 449, 922
ScaI	AGTACT	1 cut(s)	128
SchI	GAGTC	4 cut(s)	569, 1003, 1112, 1215
ScrFI	CCNGG	3 cut(s)	64, 372, 453
SduI	GDGCHC	1 cut(s)	1212
SfaNI	GCATC	6 cut(s)	145, 424, 571, 593, 709, 1183
SinI	GGWCC	2 cut(s)	297, 449
SmiMI	CAYNNNNRTG	1 cut(s)	153
SmlI	CTYRAG	2 cut(s)	647, 776
SmoI	CTYRAG	2 cut(s)	647, 776
SphI	GCATGC	2 cut(s)	407, 807
Sse9I	AATT	2 cut(s)	260, 339
SsiI	CCGC	2 cut(s)	920, 1083
SstI	GAGCTC	1 cut(s)	1212
StyD4I	CCNGG	3 cut(s)	62, 370, 451
StyI	CCWWGG	2 cut(s)	136, 925
TaaI	ACNGT	6 cut(s)	152, 325, 425, 746, 934, 1099
TaiI	ACGT	1 cut(s)	1169
TaqI	TCGA	6 cut(s)	39, 132, 587, 710, 1157, 1191
TasI	AATT	2 cut(s)	260, 339
TatI	WGTACW	3 cut(s)	126, 204, 546
TauI	GCSGC	2 cut(s)	923, 1085
TfiI	GAWTC	3 cut(s)	666, 729, 852
Tru1I	TTAA	3 cut(s)	648, 777, 1231
Tru9I	TTAA	3 cut(s)	648, 777, 1231
TscAI	CASTG	5 cut(s)	436, 559, 601, 1072, 1104
TseFI	GTSAC	4 cut(s)	253, 858, 934, 941
TseI	GCWGC	2 cut(s)	722, 1202
Tsp45I	GTSAC	4 cut(s)	253, 858, 934, 941
TspDTI	ATGAA	7 cut(s)	69, 258, 605, 708, 753, 944, 1177
TspGWI	ACGGA	3 cut(s)	176, 272, 557
TspRI	CASTG	5 cut(s)	436, 559, 601, 1072, 1104
Vha464I	CTTAAG	2 cut(s)	647, 776
VpaK11BI	GGWCC	2 cut(s)	297, 449
XapI	RAATTY	1 cut(s)	260
XceI	RCATGY	2 cut(s)	407, 807
XcmI	CCANNNNNNNNNTGG	1 cut(s)	40
ZrmI	AGTACT	1 cut(s)	128
Zsp2I	ATGCAT	1 cut(s)	586

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.