Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0005226

Physical Location & Seq

Genomic Coordinates Reverse (-)

35378807 .. 35381366

Gene Structure 2560 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1276900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1068 bp

ATGACAAGCGTTTTCTGGAAATCCATTAAGGACAAGCTTATTTTTCCATTTGTGGAATTGGACATCAAATACTTTGACCTTGGTCTTCCTCATCGGGATGCCACTGATGACAAGGTTACTGTTGAAAGTGCTGAGGCTACTCTCAAGTACAATGTAGAGATCAAGTGTGCAACTATTACTACAGATGAAGCTCGTATGAAGGAGTTTAACTTGAAGAGTGTGTGGAGGAGTCCCAATGGGACTATTAGGAATATTTTGAATGATACTGTTTTCAGAGAACCAATTATTTGCAAAAACATCCCTCGCCTTATCCCAGGCTGGACAAAGCCGATATGCATTGGAAGACATGCTTTTGGTGATCAGTATCGAGCAACTGATGCAGTCATTAAAGGACATGGGAAACTAAAATTGGTGTTTGTGCCAGAAGGAAAGGATGAGAAGACAGAGCTAGAGGTTTACAACTTTACAGGGGAGGGGGGAGTTGCATTGGCCATGTACAACACTGATGACTCCATCCGTGCTTTTGCAGAGGCTTCCATGACCACAGCTTATGAGAAAAAATTTAAGGACATATTTCAACAAGTTTATGAAGCTAACTGGAAATCGAAGTTTGAAGCTGCTGGAATATGGTATGAGCATCGTCTCATTGATGATATGGTGGCTTTTGCACTTAAAAGTGGTGGTGGGTATGTTTGGGCATGCAAGAATTATGATGGAGATGTGCAAAGTGATATGTTAGCTCAAGGGTTTGGATCTCTTGGATTGATGACATCAGTACTGGTGTGCCCAGATGGAAAGACTATAGAAGCTGAAGCTGCCCACGGTACAGTTACTCGGCATTACAGGGTTCACCAGAAGGGAGGCGAACGATTGGATGACAATGCAAGAGTTTTTGAGTTCACTCAAAAACTCGAAGAAGCTTGTATTGGAACTGTGGAATCGGGGAAAATGACCAAGGATCTTGCATTAATTCTTCACGGATCCAAGCTGGCTAGGAACCACTACTTGAACACTGAAGAATTCATTGTTGCTGTGGCCGAGGAACTGAAAGCTAAGCTTGCTTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004448 GO:0004450 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006102 GO:0006139 GO:0006725 GO:0006732 GO:0006733 GO:0006739 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009117 GO:0009506 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009651 GO:0009987 GO:0010035 GO:0010038 GO:0010043 GO:0016020 GO:0016491 GO:0016614 GO:0016616 GO:0019362 GO:0019637 GO:0019752 GO:0030054 GO:0034641 GO:0042221 GO:0042742 GO:0043167 GO:0043169 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046496 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050896 GO:0051186 GO:0051704 GO:0051707 GO:0055044 GO:0055086 GO:0055114 GO:0071704 GO:0071944 GO:0072350 GO:0072524 GO:0098542 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00020 ko00480 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 ko04146 map00020 map00480 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230 map04146

Pfam Domains

Protein Families

Iso_dh

Protein Analysis

356

Amino Acids

39.91

Weight (kDa)

6.04

Isoelectric Point (pI)

24.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Iso_dh	PF00180	2 - 344	2.2e-49	Isocitrate/isopropylmalate dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005226)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65930
fragaria_vesca	FvH4_1g27490 FvH4_1g27490 FvH4_1g30530 FvH4_1g30530
malus_domestica	MD09G1276900.v1.1 MD09G1277300.v1.1 MD17G1270700.v1.1
prunus_persica	Prupe.3G015500_v2.0.a1 Prupe.3G015500_v2.0.a1 Prupe.3G015500_v2.0.a1 Prupe.3G015500_v2.0.a1
pyrus_communis	pycom09g18670 pycom17g26910
rosa_chinensis	RchiOBHm_Chr3g0489041 RchiOBHm_Chr3g0489051
rosa_laevigata	RLG00000022897 RLG00000022898
rosa_multiflora	Rmu_sc0002227.1_g000003 Rmu_sc0011911.1_g000008
rosa_roxburghii	Rroxscaffold_6G00393730
rosa_rugosa	Rorug03G0242800 Rorug03G0242900 Rorug03G0242900
rosa_samantha	Rh3AG291100 Rh3BG327800 Rh3DG324200
rosa_wichuraiana	Rw3G025760 Rw3G025770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AclWI	GGATC	4 cut(s)	760, 966, 975, 988
AcoI	YGGCCR	2 cut(s)	489, 1035
AcsI	RAATTY	2 cut(s)	560, 1019
AcuI	CTGAAG	2 cut(s)	831, 1035
AfaI	GTAC	4 cut(s)	149, 497, 777, 826
AgsI	TTSAA	6 cut(s)	125, 214, 259, 578, 614, 1009
AjnI	CCWGG	1 cut(s)	313
AjuI	GAANNNNNNNTTGG	4 cut(s)	392, 424, 909, 941
Alw26I	GTCTC	1 cut(s)	647
AlwI	GGATC	4 cut(s)	760, 966, 975, 988
AoxI	GGCC	2 cut(s)	489, 1035
ApeKI	GCWGC	2 cut(s)	617, 815
ApoI	RAATTY	2 cut(s)	560, 1019
AseI	ATTAAT	1 cut(s)	968
AsuHPI	GGTGA	2 cut(s)	368, 842
BaeGI	GKGCMC	1 cut(s)	788
BalI	TGGCCA	1 cut(s)	491
BamHI	GGATCC	1 cut(s)	980
BbsI	GAAGAC	3 cut(s)	77, 349, 446
BbvCI	CCTCAGC	1 cut(s)	132
BbvI	GCAGC	2 cut(s)	604, 802
BccI	CCATC	3 cut(s)	521, 707, 785
BciT130I	CCWGG	1 cut(s)	315
BclI	TGATCA	1 cut(s)	358
BcoDI	GTCTC	1 cut(s)	647
BfaI	CTAG	3 cut(s)	449, 993, 1066
BfmI	CTRYAG	2 cut(s)	180, 801
BisI	GCNGC	2 cut(s)	618, 816
BlpI	GCTNAGC	1 cut(s)	1053
BlsI	GCNGC	2 cut(s)	619, 817
BmcAI	AGTACT	1 cut(s)	777
Bme1390I	CCNGG	1 cut(s)	315
BmiI	GGNNCC	2 cut(s)	982, 998
BmrFI	CCNGG	1 cut(s)	315
BmsI	GCATC	3 cut(s)	88, 367, 646
BoxI	GACNNNNGTC	1 cut(s)	81
BpiI	GAAGAC	3 cut(s)	77, 349, 446
Bpu10I	CCTNAGC	1 cut(s)	132
Bpu1102I	GCTNAGC	1 cut(s)	1053
BpuEI	CTTGAG	2 cut(s)	128, 726
BsaBI	GATNNNNATC	1 cut(s)	363
BsaJI	CCNNGG	5 cut(s)	79, 313, 820, 954, 1038
Bse1I	ACTGG	2 cut(s)	602, 783
Bse8I	GATNNNNATC	1 cut(s)	363
BseBI	CCWGG	1 cut(s)	315
BseDI	CCNNGG	5 cut(s)	79, 313, 820, 954, 1038
BseGI	GGATG	5 cut(s)	103, 297, 439, 513, 880
BseJI	GATNNNNATC	1 cut(s)	363
BseMII	CTCAG	1 cut(s)	123
BseNI	ACTGG	2 cut(s)	602, 783
BseRI	GAGGAG	1 cut(s)	241
BseSI	GKGCMC	1 cut(s)	788
BseXI	GCAGC	2 cut(s)	604, 802
BshFI	GGCC	2 cut(s)	491, 1037
BslFI	GGGAC	2 cut(s)	216, 253
BsmAI	GTCTC	1 cut(s)	647
BsmBI	CGTCTC	1 cut(s)	647
BsmFI	GGGAC	2 cut(s)	216, 253
BsnI	GGCC	2 cut(s)	491, 1037
Bsp1286I	GDGCHC	1 cut(s)	788
Bsp1407I	TGTACA	1 cut(s)	495
Bsp143I	GATC	5 cut(s)	159, 358, 752, 958, 980
Bsp1720I	GCTNAGC	1 cut(s)	1053
BspANI	GGCC	2 cut(s)	491, 1037
BspCNI	CTCAG	1 cut(s)	124
BspLI	GGNNCC	2 cut(s)	982, 998
BspPI	GGATC	4 cut(s)	760, 966, 975, 988
BsrGI	TGTACA	1 cut(s)	495
BsrI	ACTGG	2 cut(s)	602, 783
BssECI	CCNNGG	5 cut(s)	79, 313, 820, 954, 1038
BssMI	GATC	5 cut(s)	159, 358, 752, 958, 980
BssT1I	CCWWGG	2 cut(s)	79, 954
Bst2UI	CCWGG	1 cut(s)	315
Bst4CI	ACNGT	5 cut(s)	121, 268, 824, 829, 934
Bst6I	CTCTTC	1 cut(s)	209
BstAPI	GCANNNNNTGC	1 cut(s)	377
BstAUI	TGTACA	1 cut(s)	495
BstC8I	GCNNGC	4 cut(s)	700, 990, 1059, 1063
BstDEI	CTNAG	2 cut(s)	132, 1053
BstDSI	CCRYGG	1 cut(s)	820
BstF5I	GGATG	5 cut(s)	103, 297, 439, 513, 880
BstKTI	GATC	5 cut(s)	162, 361, 755, 961, 983
BstMAI	GTCTC	1 cut(s)	647
BstMBI	GATC	5 cut(s)	159, 358, 752, 958, 980
BstMWI	GCNNNNNNNGC	3 cut(s)	377, 815, 1058
BstNI	CCWGG	1 cut(s)	315
BstNSI	RCATGY	2 cut(s)	350, 702
BstPAI	GACNNNNGTC	1 cut(s)	81
BstSCI	CCNGG	1 cut(s)	313
BstSFI	CTRYAG	2 cut(s)	180, 801
BstSLI	GKGCMC	1 cut(s)	788
BstV1I	GCAGC	2 cut(s)	604, 802
BstV2I	GAAGAC	3 cut(s)	77, 349, 446
BstX2I	RGATCY	3 cut(s)	752, 958, 980
BstYI	RGATCY	3 cut(s)	752, 958, 980
BsuRI	GGCC	2 cut(s)	491, 1037
BtgI	CCRYGG	1 cut(s)	820
BtsCI	GGATG	5 cut(s)	103, 297, 439, 513, 880
BtsIMutI	CAGTG	3 cut(s)	102, 501, 1011
Cac8I	GCNNGC	4 cut(s)	700, 990, 1059, 1063
Csp6I	GTAC	4 cut(s)	148, 496, 776, 825
CviAII	CATG	5 cut(s)	347, 395, 493, 538, 699
CviQI	GTAC	4 cut(s)	148, 496, 776, 825
DdeI	CTNAG	2 cut(s)	132, 1053
DpnI	GATC	5 cut(s)	161, 360, 754, 960, 982
DpnII	GATC	5 cut(s)	159, 358, 752, 958, 980
EaeI	YGGCCR	2 cut(s)	489, 1035
Eam1104I	CTCTTC	1 cut(s)	209
EarI	CTCTTC	1 cut(s)	209
Eco130I	CCWWGG	2 cut(s)	79, 954
Eco57I	CTGAAG	2 cut(s)	831, 1035
EcoRI	GAATTC	1 cut(s)	1019
EcoRII	CCWGG	1 cut(s)	313
EcoT14I	CCWWGG	2 cut(s)	79, 954
EcoT22I	ATGCAT	1 cut(s)	338
ErhI	CCWWGG	2 cut(s)	79, 954
Esp3I	CGTCTC	1 cut(s)	647
FaeI	CATG	5 cut(s)	350, 398, 496, 541, 702
FalI	AAGNNNNNCTT	3 cut(s)	334, 366, 1041
FaqI	GGGAC	2 cut(s)	216, 253
FatI	CATG	5 cut(s)	346, 394, 492, 537, 698
FbaI	TGATCA	1 cut(s)	358
Fnu4HI	GCNGC	2 cut(s)	618, 816
FokI	GGATG	5 cut(s)	110, 284, 446, 500, 887
Fsp4HI	GCNGC	2 cut(s)	618, 816
FspBI	CTAG	3 cut(s)	449, 993, 1066
GluI	GCNGC	2 cut(s)	618, 816
HaeIII	GGCC	2 cut(s)	491, 1037
Hin1II	CATG	5 cut(s)	350, 398, 496, 541, 702
HindIII	AAGCTT	3 cut(s)	35, 918, 1055
HinfI	GANTC	3 cut(s)	229, 509, 938
HphI	GGTGA	2 cut(s)	368, 842
Hpy166II	GTNNAC	3 cut(s)	457, 850, 900
Hpy188I	TCNGA	1 cut(s)	275
Hpy188III	TCNNGA	2 cut(s)	16, 95
Hpy8I	GTNNAC	3 cut(s)	457, 850, 900
HpyAV	CCTTC	3 cut(s)	193, 419, 850
HpyCH4III	ACNGT	5 cut(s)	121, 268, 824, 829, 934
HpyF10VI	GCNNNNNNNGC	3 cut(s)	377, 815, 1058
HpyF3I	CTNAG	2 cut(s)	132, 1053
Hsp92II	CATG	5 cut(s)	350, 398, 496, 541, 702
Ksp22I	TGATCA	1 cut(s)	358
Kzo9I	GATC	5 cut(s)	159, 358, 752, 958, 980
Lsp1109I	GCAGC	2 cut(s)	604, 802
LweI	GCATC	3 cut(s)	88, 367, 646
MaeI	CTAG	3 cut(s)	449, 993, 1066
MaeIII	GTNAC	2 cut(s)	115, 829
MalI	GATC	5 cut(s)	161, 360, 754, 960, 982
MboI	GATC	5 cut(s)	159, 358, 752, 958, 980
MboII	GAAGA	7 cut(s)	77, 226, 354, 451, 926, 965, 1028
MflI	RGATCY	3 cut(s)	752, 958, 980
MhlI	GDGCHC	1 cut(s)	788
MlsI	TGGCCA	1 cut(s)	491
MluCI	AATT	7 cut(s)	56, 282, 407, 560, 706, 969, 1019
MluNI	TGGCCA	1 cut(s)	491
MlyI	GAGTC	2 cut(s)	238, 503
MnlI	CCTC	9 cut(s)	99, 127, 219, 312, 445, 466, 523, 854, 1033
Mox20I	TGGCCA	1 cut(s)	491
Mph1103I	ATGCAT	1 cut(s)	338
MscI	TGGCCA	1 cut(s)	491
MseI	TTAA	6 cut(s)	27, 207, 387, 564, 672, 968
MslI	CAYNNNNRTG	1 cut(s)	96
Msp20I	TGGCCA	1 cut(s)	491
MspR9I	CCNGG	1 cut(s)	315
MvaI	CCWGG	1 cut(s)	315
MwoI	GCNNNNNNNGC	3 cut(s)	377, 815, 1058
NdeII	GATC	5 cut(s)	159, 358, 752, 958, 980
NlaIII	CATG	5 cut(s)	350, 398, 496, 541, 702
NlaIV	GGNNCC	2 cut(s)	982, 998
NmeAIII	GCCGAG	2 cut(s)	814, 1063
NsiI	ATGCAT	1 cut(s)	338
NspI	RCATGY	2 cut(s)	350, 702
PaeI	GCATGC	1 cut(s)	702
PfeI	GAWTC	1 cut(s)	938
PkrI	GCNGC	2 cut(s)	619, 817
PleI	GAGTC	2 cut(s)	237, 503
PpsI	GAGTC	2 cut(s)	237, 503
PshAI	GACNNNNGTC	1 cut(s)	81
PshBI	ATTAAT	1 cut(s)	968
Psp6I	CCWGG	1 cut(s)	313
PspGI	CCWGG	1 cut(s)	313
PspN4I	GGNNCC	2 cut(s)	982, 998
PsuI	RGATCY	3 cut(s)	752, 958, 980
RsaI	GTAC	4 cut(s)	149, 497, 777, 826
RsaNI	GTAC	4 cut(s)	148, 496, 776, 825
RseI	CAYNNNNRTG	1 cut(s)	96
SaqAI	TTAA	6 cut(s)	27, 207, 387, 564, 672, 968
SatI	GCNGC	2 cut(s)	618, 816
Sau3AI	GATC	5 cut(s)	159, 358, 752, 958, 980
ScaI	AGTACT	1 cut(s)	777
SchI	GAGTC	2 cut(s)	238, 503
ScrFI	CCNGG	1 cut(s)	315
SduI	GDGCHC	1 cut(s)	788
SfaNI	GCATC	3 cut(s)	88, 367, 646
SfcI	CTRYAG	2 cut(s)	180, 801
SmiMI	CAYNNNNRTG	1 cut(s)	96
SmlI	CTYRAG	2 cut(s)	143, 741
SmoI	CTYRAG	2 cut(s)	143, 741
SphI	GCATGC	1 cut(s)	702
Sse9I	AATT	7 cut(s)	56, 282, 407, 560, 706, 969, 1019
SspI	AATATT	1 cut(s)	253
SspMI	CTAG	3 cut(s)	449, 993, 1066
StyD4I	CCNGG	1 cut(s)	313
StyI	CCWWGG	2 cut(s)	79, 954
TaaI	ACNGT	5 cut(s)	121, 268, 824, 829, 934
TaqI	TCGA	3 cut(s)	367, 605, 912
TasI	AATT	7 cut(s)	56, 282, 407, 560, 706, 969, 1019
TatI	WGTACW	3 cut(s)	147, 495, 775
TfiI	GAWTC	1 cut(s)	938
Tru1I	TTAA	6 cut(s)	27, 207, 387, 564, 672, 968
Tru9I	TTAA	6 cut(s)	27, 207, 387, 564, 672, 968
TscAI	CASTG	3 cut(s)	109, 508, 1018
TseI	GCWGC	2 cut(s)	617, 815
TspDTI	ATGAA	4 cut(s)	201, 212, 603, 1012
TspGWI	ACGGA	2 cut(s)	506, 993
TspRI	CASTG	3 cut(s)	109, 508, 1018
VspI	ATTAAT	1 cut(s)	968
XapI	RAATTY	2 cut(s)	560, 1019
XceI	RCATGY	2 cut(s)	350, 702
XspI	CTAG	3 cut(s)	449, 993, 1066
ZrmI	AGTACT	1 cut(s)	777
Zsp2I	ATGCAT	1 cut(s)	338

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.