Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0005226

Physical Location & Seq

Genomic Coordinates Reverse (-)

14620074 .. 14623928

Gene Structure 3855 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000022898

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1242 bp

ATGGAGTTCGAGAAGATCAAGGTCGCCAATCCCATCGTTGAGATGGACGGATTTAGTTTTCATCTGAATGTCTTTTCCACTTTGTTCTGGCTCATCACCTTGAATCATCTTTTACTCTTTTTAAATACATGTTATTGCCTGCCAAGAGCCCAAGATAGAACTCTTATTTTCCCCTTTTTGGATCTGGACATCAAGTACTTTGACCTTGGTCTTCCTCATCGTGATGCCACTAATGATAAAGTTACCGTTGAAAGTGCAGAGGCAACTCTTAGCATGTTAGAATTTGGGCATTCATATGTTCTTGTTGAACTCCATGTTTACCGCTTTAGTACTGTTTTCAGAGAACCGATTCTTTGCAAGAACGTTCCTCGCCTTATCCCAGGCTGGACAAAGGCAATATGCATTGGAAGACATGCTTTCGGTGATCAATATCGATCAATTGATTTAGTCATAAAAGGACCTGGGAAACTGAAATTGCTTTTTGTTCCAGAAGGAAAGGATGAGAAGACTGAACTGGATGTATACAACTTTACAGGGGAAGGGGGAGTTGCACTTGCCATGTGCAACGTTGATGAGTCAATCCGTGCTTTTGCAGAGGCTTCTATGACCACAGCTTATGAGAAAAAGTGGCCTCTTTATCTTAGCACAAAAAATACTGTTCTTAAGAAGTACGATGGAAGGTATATGCATATTCTCTATTGGCCTTTTATTAATATATATCTAGGATTTTATTTATCATTCTCCAAGTATGAACATCGTCTCATTGATGATATGGTGGCTTATGCACTTAAAAGCGAAGGTGGTTTTGTTTGGGCATGCAAGAATTATGATGGAGATGTACAGAGTGATTTCTTAGCCCAAGGTTTTGGATCTCTTGGATTGATGACTTCAGTACTGGTGTGCCCAGATGGAAAGACTATAGAAGCTGAAGCTGCCCATGGTACAGTTACTCGCCATTATAGAATCCACCAAAAAGGAGGTGAAACCAGCACAAACAGCATAGCTTCTATTTTTGCTTGGACAAGAGGGCTTGCACACAGGGCAAAGTTGGATGAAAATGCTAAACTGTTGGATTTCACACAGAAGCTGGAAGCAGCTTGTATTGGTACTGTGGAATCTGGGAAAATGACGAAGGATCTTGCGCTAAATATTCATGGACCTAAGGTTGCTAGGAACCACTACTTGAACACGGAAGAGTTCATTGATGCCGTCGCAGCAGAACTGAGAGCAAAGCTTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004448 GO:0004450 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006102 GO:0006139 GO:0006725 GO:0006732 GO:0006733 GO:0006739 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009117 GO:0009506 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009651 GO:0009987 GO:0010035 GO:0010038 GO:0010043 GO:0016020 GO:0016491 GO:0016614 GO:0016616 GO:0019362 GO:0019637 GO:0019752 GO:0030054 GO:0034641 GO:0042221 GO:0042742 GO:0043167 GO:0043169 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046496 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050896 GO:0051186 GO:0051704 GO:0051707 GO:0055044 GO:0055086 GO:0055114 GO:0071704 GO:0071944 GO:0072350 GO:0072524 GO:0098542 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00020 ko00480 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 ko04146 map00020 map00480 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230 map04146

Pfam Domains

Protein Families

Iso_dh

Protein Analysis

414

Amino Acids

46.7

Weight (kDa)

6.4

Isoelectric Point (pI)

25.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Iso_dh	PF00180	111 - 404	2.6e-50	Isocitrate/isopropylmalate dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005226)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65930
fragaria_vesca	FvH4_1g27490 FvH4_1g27490 FvH4_1g30530 FvH4_1g30530
malus_domestica	MD09G1276900.v1.1 MD09G1277300.v1.1 MD17G1270700.v1.1
prunus_persica	Prupe.3G015500_v2.0.a1 Prupe.3G015500_v2.0.a1 Prupe.3G015500_v2.0.a1 Prupe.3G015500_v2.0.a1
pyrus_communis	pycom09g18670 pycom17g26910
rosa_chinensis	RchiOBHm_Chr3g0489041 RchiOBHm_Chr3g0489051
rosa_laevigata	RLG00000022897 RLG00000022898
rosa_multiflora	Rmu_sc0002227.1_g000003 Rmu_sc0011911.1_g000008
rosa_roxburghii	Rroxscaffold_6G00393730
rosa_rugosa	Rorug03G0242800 Rorug03G0242900 Rorug03G0242900
rosa_samantha	Rh3AG291100 Rh3BG327800 Rh3DG324200
rosa_wichuraiana	Rw3G025760 Rw3G025770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	522
AciI	CCGC	1 cut(s)	322
AclI	AACGTT	2 cut(s)	363, 567
AclWI	GGATC	3 cut(s)	189, 877, 1143
AcsI	RAATTY	1 cut(s)	281
AcuI	CTGAAG	2 cut(s)	873, 948
AfaI	GTAC	7 cut(s)	197, 331, 671, 840, 894, 943, 1108
AfiI	CCNNNNNNNGG	1 cut(s)	178
AflII	CTTAAG	1 cut(s)	662
AflIII	ACRYGT	1 cut(s)	128
AgsI	TTSAA	4 cut(s)	103, 251, 308, 1186
AjnI	CCWGG	2 cut(s)	379, 460
AluBI	AGCT	7 cut(s)	614, 926, 932, 1004, 1087, 1097, 1234
AluI	AGCT	7 cut(s)	614, 926, 932, 1004, 1087, 1097, 1234
Alw26I	GTCTC	1 cut(s)	764
AlwI	GGATC	3 cut(s)	189, 877, 1143
AlwNI	CAGNNNCTG	1 cut(s)	1087
AoxI	GGCC	2 cut(s)	629, 701
ApeKI	GCWGC	3 cut(s)	932, 1094, 1214
ApoI	RAATTY	1 cut(s)	281
AseI	ATTAAT	1 cut(s)	711
Asp700I	GAANNNNTTC	1 cut(s)	348
AspLEI	GCGC	1 cut(s)	1144
AspS9I	GGNCC	2 cut(s)	458, 1157
AsuHPI	GGTGA	3 cut(s)	88, 434, 992
AvaII	GGWCC	2 cut(s)	458, 1157
AxyI	CCTNAGG	1 cut(s)	1161
BaeGI	GKGCMC	1 cut(s)	905
BanII	GRGCYC	1 cut(s)	151
BbsI	GAAGAC	3 cut(s)	203, 415, 512
BbvI	GCAGC	3 cut(s)	919, 1106, 1226
BccI	CCATC	5 cut(s)	37, 41, 668, 824, 902
BceAI	ACGGC	1 cut(s)	1193
BciT130I	CCWGG	2 cut(s)	381, 462
BclI	TGATCA	1 cut(s)	424
BcoDI	GTCTC	1 cut(s)	764
BfaI	CTAG	2 cut(s)	722, 1170
BfmI	CTRYAG	1 cut(s)	918
BfrI	CTTAAG	1 cut(s)	662
BisI	GCNGC	3 cut(s)	933, 1095, 1215
BlsI	GCNGC	3 cut(s)	934, 1096, 1216
BmcAI	AGTACT	3 cut(s)	197, 331, 894
Bme1390I	CCNGG	2 cut(s)	381, 462
Bme18I	GGWCC	2 cut(s)	458, 1157
BmgT120I	GGNCC	2 cut(s)	458, 1157
BmiI	GGNNCC	1 cut(s)	1175
BmrFI	CCNGG	2 cut(s)	381, 462
BmsI	GCATC	2 cut(s)	214, 1195
BoxI	GACNNNNGTC	1 cut(s)	207
BpiI	GAAGAC	3 cut(s)	203, 415, 512
Bsa29I	ATCGAT	1 cut(s)	433
BsaBI	GATNNNNATC	1 cut(s)	429
BsaJI	CCNNGG	5 cut(s)	205, 379, 461, 859, 937
Bsc4I	CCNNNNNNNGG	1 cut(s)	178
Bse1I	ACTGG	2 cut(s)	519, 900
Bse21I	CCTNAGG	1 cut(s)	1161
Bse8I	GATNNNNATC	1 cut(s)	429
BseBI	CCWGG	2 cut(s)	381, 462
BseCI	ATCGAT	1 cut(s)	433
BseDI	CCNNGG	5 cut(s)	205, 379, 461, 859, 937
BseGI	GGATG	3 cut(s)	505, 523, 1057
BseJI	GATNNNNATC	1 cut(s)	429
BseLI	CCNNNNNNNGG	1 cut(s)	178
BseMII	CTCAG	1 cut(s)	1214
BseNI	ACTGG	2 cut(s)	519, 900
BseSI	GKGCMC	1 cut(s)	905
BseXI	GCAGC	3 cut(s)	919, 1106, 1226
BsgI	GTGCAG	1 cut(s)	276
BshFI	GGCC	2 cut(s)	631, 703
BshVI	ATCGAT	1 cut(s)	433
BslI	CCNNNNNNNGG	1 cut(s)	178
BsmAI	GTCTC	1 cut(s)	764
BsmBI	CGTCTC	1 cut(s)	764
BsmI	GAATGC	1 cut(s)	289
BsnI	GGCC	2 cut(s)	631, 703
Bsp1286I	GDGCHC	2 cut(s)	151, 905
Bsp1407I	TGTACA	1 cut(s)	838
Bsp143I	GATC	6 cut(s)	15, 181, 424, 434, 869, 1135
Bsp19I	CCATGG	1 cut(s)	937
BspACI	CCGC	1 cut(s)	322
BspANI	GGCC	2 cut(s)	631, 703
BspCNI	CTCAG	1 cut(s)	1215
BspDI	ATCGAT	1 cut(s)	433
BspLI	GGNNCC	1 cut(s)	1175
BspPI	GGATC	3 cut(s)	189, 877, 1143
BspTI	CTTAAG	1 cut(s)	662
BsrGI	TGTACA	1 cut(s)	838
BsrI	ACTGG	2 cut(s)	519, 900
BssECI	CCNNGG	5 cut(s)	205, 379, 461, 859, 937
BssMI	GATC	6 cut(s)	15, 181, 424, 434, 869, 1135
BssNAI	GTATAC	1 cut(s)	523
BssT1I	CCWWGG	3 cut(s)	205, 859, 937
Bst1107I	GTATAC	1 cut(s)	523
Bst2UI	CCWGG	2 cut(s)	381, 462
Bst4CI	ACNGT	6 cut(s)	247, 334, 658, 946, 1068, 1111
Bst6I	CTCTTC	1 cut(s)	1188
BstAFI	CTTAAG	1 cut(s)	662
BstAUI	TGTACA	1 cut(s)	838
BstC8I	GCNNGC	3 cut(s)	140, 817, 1032
BstDEI	CTNAG	5 cut(s)	269, 641, 853, 1161, 1223
BstDSI	CCRYGG	1 cut(s)	937
BstF5I	GGATG	3 cut(s)	505, 523, 1057
BstHHI	GCGC	1 cut(s)	1144
BstKTI	GATC	6 cut(s)	18, 184, 427, 437, 872, 1138
BstMAI	GTCTC	1 cut(s)	764
BstMBI	GATC	6 cut(s)	15, 181, 424, 434, 869, 1135
BstMWI	GCNNNNNNNGC	4 cut(s)	932, 996, 1040, 1214
BstNI	CCWGG	2 cut(s)	381, 462
BstNSI	RCATGY	4 cut(s)	132, 277, 416, 819
BstPAI	GACNNNNGTC	1 cut(s)	207
BstSCI	CCNGG	2 cut(s)	379, 460
BstSFI	CTRYAG	1 cut(s)	918
BstSLI	GKGCMC	1 cut(s)	905
BstV1I	GCAGC	3 cut(s)	919, 1106, 1226
BstV2I	GAAGAC	3 cut(s)	203, 415, 512
BstX2I	RGATCY	3 cut(s)	181, 869, 1135
BstXI	CCANNNNNNTGG	1 cut(s)	866
BstYI	RGATCY	3 cut(s)	181, 869, 1135
BstZ17I	GTATAC	1 cut(s)	523
Bsu15I	ATCGAT	1 cut(s)	433
Bsu36I	CCTNAGG	1 cut(s)	1161
BsuRI	GGCC	2 cut(s)	631, 703
BsuTUI	ATCGAT	1 cut(s)	433
BtgI	CCRYGG	1 cut(s)	937
BtsCI	GGATG	3 cut(s)	505, 523, 1057
Cac8I	GCNNGC	3 cut(s)	140, 817, 1032
CaiI	CAGNNNCTG	1 cut(s)	1087
CfoI	GCGC	1 cut(s)	1144
Cfr13I	GGNCC	2 cut(s)	458, 1157
ClaI	ATCGAT	1 cut(s)	433
Csp6I	GTAC	7 cut(s)	196, 330, 670, 839, 893, 942, 1107
CviAII	CATG	8 cut(s)	129, 274, 314, 413, 559, 816, 938, 1154
CviQI	GTAC	7 cut(s)	196, 330, 670, 839, 893, 942, 1107
DdeI	CTNAG	5 cut(s)	269, 641, 853, 1161, 1223
DpnI	GATC	6 cut(s)	17, 183, 426, 436, 871, 1137
DpnII	GATC	6 cut(s)	15, 181, 424, 434, 869, 1135
DraI	TTTAAA	1 cut(s)	123
Eam1104I	CTCTTC	1 cut(s)	1188
EarI	CTCTTC	1 cut(s)	1188
Eco130I	CCWWGG	3 cut(s)	205, 859, 937
Eco24I	GRGCYC	1 cut(s)	151
Eco47I	GGWCC	2 cut(s)	458, 1157
Eco57I	CTGAAG	2 cut(s)	873, 948
Eco81I	CCTNAGG	1 cut(s)	1161
EcoO109I	RGGNCCY	1 cut(s)	458
EcoRII	CCWGG	2 cut(s)	379, 460
EcoT14I	CCWWGG	3 cut(s)	205, 859, 937
EcoT22I	ATGCAT	2 cut(s)	404, 690
EcoT38I	GRGCYC	1 cut(s)	151
ErhI	CCWWGG	3 cut(s)	205, 859, 937
Esp3I	CGTCTC	1 cut(s)	764
FaeI	CATG	8 cut(s)	132, 277, 317, 416, 562, 819, 941, 1157
FalI	AAGNNNNNCTT	2 cut(s)	400, 432
FatI	CATG	8 cut(s)	128, 273, 313, 412, 558, 815, 937, 1153
FauNDI	CATATG	1 cut(s)	295
FbaI	TGATCA	1 cut(s)	424
FblI	GTMKAC	1 cut(s)	522
Fnu4HI	GCNGC	3 cut(s)	933, 1095, 1215
FokI	GGATG	3 cut(s)	512, 530, 1064
FriOI	GRGCYC	1 cut(s)	151
Fsp4HI	GCNGC	3 cut(s)	933, 1095, 1215
FspBI	CTAG	2 cut(s)	722, 1170
GlaI	GCGC	1 cut(s)	1143
GluI	GCNGC	3 cut(s)	933, 1095, 1215
HaeIII	GGCC	2 cut(s)	631, 703
HhaI	GCGC	1 cut(s)	1144
Hin1II	CATG	8 cut(s)	132, 277, 317, 416, 562, 819, 941, 1157
Hin6I	GCGC	1 cut(s)	1142
HinP1I	GCGC	1 cut(s)	1142
HindIII	AAGCTT	1 cut(s)	1232
HinfI	GANTC	5 cut(s)	103, 349, 575, 963, 1115
HphI	GGTGA	3 cut(s)	88, 434, 992
Hpy166II	GTNNAC	2 cut(s)	319, 523
Hpy188I	TCNGA	2 cut(s)	66, 341
Hpy188III	TCNNGA	5 cut(s)	10, 185, 221, 488, 1239
Hpy8I	GTNNAC	2 cut(s)	319, 523
Hpy99I	CGWCG	1 cut(s)	1214
HpyAV	CCTTC	5 cut(s)	485, 533, 672, 791, 1126
HpyCH4III	ACNGT	6 cut(s)	247, 334, 658, 946, 1068, 1111
HpyCH4IV	ACGT	2 cut(s)	363, 567
HpyF10VI	GCNNNNNNNGC	4 cut(s)	932, 996, 1040, 1214
HpyF3I	CTNAG	5 cut(s)	269, 641, 853, 1161, 1223
HpySE526I	ACGT	2 cut(s)	363, 567
Hsp92II	CATG	8 cut(s)	132, 277, 317, 416, 562, 819, 941, 1157
HspAI	GCGC	1 cut(s)	1142
Ksp22I	TGATCA	1 cut(s)	424
Kzo9I	GATC	6 cut(s)	15, 181, 424, 434, 869, 1135
Lsp1109I	GCAGC	3 cut(s)	919, 1106, 1226
LweI	GCATC	2 cut(s)	214, 1195
MaeI	CTAG	2 cut(s)	722, 1170
MaeII	ACGT	2 cut(s)	363, 567
MaeIII	GTNAC	2 cut(s)	241, 946
MalI	GATC	6 cut(s)	17, 183, 426, 436, 871, 1137
MboI	GATC	6 cut(s)	15, 181, 424, 434, 869, 1135
MboII	GAAGA	5 cut(s)	25, 203, 420, 517, 1205
MfeI	CAATTG	1 cut(s)	438
MflI	RGATCY	3 cut(s)	181, 869, 1135
MhlI	GDGCHC	2 cut(s)	151, 905
MluCI	AATT	4 cut(s)	281, 438, 473, 823
MlyI	GAGTC	1 cut(s)	584
MmeI	TCCRAC	2 cut(s)	1029, 1050
MnlI	CCTC	7 cut(s)	225, 253, 378, 589, 642, 971, 1019
Mph1103I	ATGCAT	2 cut(s)	404, 690
MroXI	GAANNNNTTC	1 cut(s)	348
MseI	TTAA	4 cut(s)	122, 663, 711, 789
MslI	CAYNNNNRTG	3 cut(s)	66, 222, 294
MspCI	CTTAAG	1 cut(s)	662
MspR9I	CCNGG	2 cut(s)	381, 462
MunI	CAATTG	1 cut(s)	438
Mva1269I	GAATGC	1 cut(s)	289
MvaI	CCWGG	2 cut(s)	381, 462
MwoI	GCNNNNNNNGC	4 cut(s)	932, 996, 1040, 1214
NcoI	CCATGG	1 cut(s)	937
NdeI	CATATG	1 cut(s)	295
NdeII	GATC	6 cut(s)	15, 181, 424, 434, 869, 1135
NlaIII	CATG	8 cut(s)	132, 277, 317, 416, 562, 819, 941, 1157
NlaIV	GGNNCC	1 cut(s)	1175
NsiI	ATGCAT	2 cut(s)	404, 690
NspI	RCATGY	4 cut(s)	132, 277, 416, 819
PaeI	GCATGC	1 cut(s)	819
PciI	ACATGT	1 cut(s)	128
PctI	GAATGC	1 cut(s)	289
PdmI	GAANNNNTTC	1 cut(s)	348
PfeI	GAWTC	4 cut(s)	103, 349, 963, 1115
PkrI	GCNGC	3 cut(s)	934, 1096, 1216
PleI	GAGTC	1 cut(s)	583
PpsI	GAGTC	1 cut(s)	583
PpuMI	RGGWCCY	1 cut(s)	458
PscI	ACATGT	1 cut(s)	128
PshAI	GACNNNNGTC	1 cut(s)	207
PshBI	ATTAAT	1 cut(s)	711
Psp1406I	AACGTT	2 cut(s)	363, 567
Psp5II	RGGWCCY	1 cut(s)	458
Psp6I	CCWGG	2 cut(s)	379, 460
PspGI	CCWGG	2 cut(s)	379, 460
PspN4I	GGNNCC	1 cut(s)	1175
PspPI	GGNCC	2 cut(s)	458, 1157
PspPPI	RGGWCCY	1 cut(s)	458
PstNI	CAGNNNCTG	1 cut(s)	1087
PsuI	RGATCY	3 cut(s)	181, 869, 1135
RsaI	GTAC	7 cut(s)	197, 331, 671, 840, 894, 943, 1108
RsaNI	GTAC	7 cut(s)	196, 330, 670, 839, 893, 942, 1107
RseI	CAYNNNNRTG	3 cut(s)	66, 222, 294
SaqAI	TTAA	4 cut(s)	122, 663, 711, 789
SatI	GCNGC	3 cut(s)	933, 1095, 1215
Sau3AI	GATC	6 cut(s)	15, 181, 424, 434, 869, 1135
Sau96I	GGNCC	2 cut(s)	458, 1157
ScaI	AGTACT	3 cut(s)	197, 331, 894
SchI	GAGTC	1 cut(s)	584
ScrFI	CCNGG	2 cut(s)	381, 462
SduI	GDGCHC	2 cut(s)	151, 905
SfaNI	GCATC	2 cut(s)	214, 1195
SfcI	CTRYAG	1 cut(s)	918
SinI	GGWCC	2 cut(s)	458, 1157
SmiMI	CAYNNNNRTG	3 cut(s)	66, 222, 294
SmlI	CTYRAG	1 cut(s)	662
SmoI	CTYRAG	1 cut(s)	662
SphI	GCATGC	1 cut(s)	819
Sse9I	AATT	4 cut(s)	281, 438, 473, 823
SsiI	CCGC	1 cut(s)	322
SspI	AATATT	1 cut(s)	1150
SspMI	CTAG	2 cut(s)	722, 1170
StyD4I	CCNGG	2 cut(s)	379, 460
StyI	CCWWGG	3 cut(s)	205, 859, 937
TaaI	ACNGT	6 cut(s)	247, 334, 658, 946, 1068, 1111
TaiI	ACGT	2 cut(s)	366, 570
TaqI	TCGA	2 cut(s)	9, 433
TasI	AATT	4 cut(s)	281, 438, 473, 823
TatI	WGTACW	4 cut(s)	195, 329, 838, 892
TfiI	GAWTC	4 cut(s)	103, 349, 963, 1115
Tru1I	TTAA	4 cut(s)	122, 663, 711, 789
Tru9I	TTAA	4 cut(s)	122, 663, 711, 789
TseI	GCWGC	3 cut(s)	932, 1094, 1214
TspDTI	ATGAA	6 cut(s)	50, 282, 765, 1068, 1142, 1189
TspGWI	ACGGA	3 cut(s)	63, 572, 1205
Vha464I	CTTAAG	1 cut(s)	662
VpaK11BI	GGWCC	2 cut(s)	458, 1157
VspI	ATTAAT	1 cut(s)	711
XapI	RAATTY	1 cut(s)	281
XceI	RCATGY	4 cut(s)	132, 277, 416, 819
XcmI	CCANNNNNNNNNTGG	1 cut(s)	40
XmiI	GTMKAC	1 cut(s)	522
XmnI	GAANNNNTTC	1 cut(s)	348
XspI	CTAG	2 cut(s)	722, 1170
ZrmI	AGTACT	3 cut(s)	197, 331, 894
Zsp2I	ATGCAT	2 cut(s)	404, 690

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.