Basic Information

Type: Sequence Only

Biological Identity

Species

rosa_samantha

Chromosome

Unknown

Orthogroup ID

OG0005226

Physical Location & Seq

Genomic Coordinates Reverse (-)

0 .. 0

Gene Structure 1 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3BG327800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1236 bp

ATGGAGTTCGAGAAGATCAAGGTCGCCAATCCCATCGTTGAGATGGACGGAGATGAGATGACTCGAGTTTTCTGGAAATCAATTAAGGATAAGCTTATTTTCCCCTTTTTGGATCTGGACATCAAGTACTTTGACCTTGGTCTTCCTCATCGTGATGCCACTAATGATAAAGTTACTGTTGAAAGTGCAGAGGCAACTCTTAGGTACAATGTAGCAATCAAATGCGCCACTGTTTCGACAGATGCAAATCGCATGAAGGAGTATAACTTGAAGCAACTGTGGAGGAGTCCCAATGTGACTATTAGGAACATTTTGAATGGTACTGTTTTCAGAGAACCGATTCTTTGCAAGAACGTTCCTCGCCTTATCCCAGGCTGGACAAAGACAATATGCATTGGAAGACATGCTTTCGGTGATCAATATCGATCAATTGATTTAGTCATAAAAGGACCTGGGAAACTGAAATTGCTTTTTGTTCCAGAAGGAAAGGATGAGAAGACTGAACTGGATGTATACAACTTTACAGGGGAAGGGGGAGTTGCACTTGCCATGTGCAACGTTGATGAGTCAATCCGTGCTTTTGCAGAGGCTTCTATGACCACAGCTTATGAGAAAAAGTGGCCACTTTATCTTAGCACAAAAAATACTGTTCTTAAGAAGTACGATGGAAGATTCAAGGACATATTTCAAGAAGTATATGAAGCCCAGTGGAAATCTAAGTATGAAGCTGCTGGCATATGGTATGAACATCGTCTCATTGATGATATGGTGGCTTATGCACTTAAAAGTGAAGGTGGTTATGTTTGGGCATGCAAGAATTATGATGGAGATGTGCAAAGTGATTTCTTAGCTCAAGGTTTTGGATCTCTTGGATTGATGACATCAGTACTGGTGTGCCCAGATGGAAAGACCATAGAAGCTGAAGCTGCTCATGGTACAGTTACTCGGCATTATAGAGTTCACCAGAAAGGTGGTGAAACCAGCACAAACAGCATAGCTTCAATTTTTGCTTGGACAAGAGGGCTTGCACACAGGGCAAAGTTGGATGACAATGCTAAATTGTTGGATTTCACTCAGAAGCTGGAAGAAGCTTGTATTGGAACTGTGGAATCTGGGAAAATGACCAAGGATCTTGCACTAATTCTTCATGGACCTAAGCTTGCTAGGAACCACTACTTGAACACGGAGGAGTTCATTGATGCCGTGGCTGCAGAACTGAAAGCAAAGCTTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004448 GO:0004450 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006102 GO:0006139 GO:0006725 GO:0006732 GO:0006733 GO:0006739 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009117 GO:0009506 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009651 GO:0009987 GO:0010035 GO:0010038 GO:0010043 GO:0016020 GO:0016491 GO:0016614 GO:0016616 GO:0019362 GO:0019637 GO:0019752 GO:0030054 GO:0034641 GO:0042221 GO:0042742 GO:0043167 GO:0043169 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046496 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050896 GO:0051186 GO:0051704 GO:0051707 GO:0055044 GO:0055086 GO:0055114 GO:0071704 GO:0071944 GO:0072350 GO:0072524 GO:0098542 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00020 ko00480 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 ko04146 map00020 map00480 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230 map04146

Pfam Domains

Protein Families

Iso_dh

Protein Analysis

411

Amino Acids

46.34

Weight (kDa)

6.77

Isoelectric Point (pI)

20.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Iso_dh	PF00180	7 - 402	1e-78	Isocitrate/isopropylmalate dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005226)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65930
fragaria_vesca	FvH4_1g27490 FvH4_1g27490 FvH4_1g30530 FvH4_1g30530
malus_domestica	MD09G1276900.v1.1 MD09G1277300.v1.1 MD17G1270700.v1.1
prunus_persica	Prupe.3G015500_v2.0.a1 Prupe.3G015500_v2.0.a1 Prupe.3G015500_v2.0.a1 Prupe.3G015500_v2.0.a1
pyrus_communis	pycom09g18670 pycom17g26910
rosa_chinensis	RchiOBHm_Chr3g0489041 RchiOBHm_Chr3g0489051
rosa_laevigata	RLG00000022897 RLG00000022898
rosa_multiflora	Rmu_sc0002227.1_g000003 Rmu_sc0011911.1_g000008
rosa_roxburghii	Rroxscaffold_6G00393730
rosa_rugosa	Rorug03G0242800 Rorug03G0242900 Rorug03G0242900
rosa_samantha	Rh3AG291100 Rh3BG327800 Rh3DG324200
rosa_wichuraiana	Rw3G025760 Rw3G025770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	513
AclI	AACGTT	2 cut(s)	354, 558
AclWI	GGATC	3 cut(s)	120, 871, 1137
AcoI	YGGCCR	1 cut(s)	620
AcuI	CTGAAG	1 cut(s)	942
AfaI	GTAC	6 cut(s)	128, 206, 322, 662, 888, 937
AfiI	CCNNNNNNNGG	1 cut(s)	109
AflII	CTTAAG	1 cut(s)	653
AgsI	TTSAA	7 cut(s)	182, 271, 316, 676, 689, 1002, 1180
AjnI	CCWGG	2 cut(s)	370, 451
AjuI	GAANNNNNNNTTGG	2 cut(s)	1080, 1112
Alw26I	GTCTC	1 cut(s)	758
AlwI	GGATC	3 cut(s)	120, 871, 1137
AlwNI	CAGNNNCTG	1 cut(s)	1081
Ama87I	CYCGRG	1 cut(s)	63
AoxI	GGCC	1 cut(s)	620
ApeKI	GCWGC	3 cut(s)	728, 926, 1208
Asp700I	GAANNNNTTC	1 cut(s)	339
AspLEI	GCGC	1 cut(s)	227
AspS9I	GGNCC	2 cut(s)	449, 1151
AsuHPI	GGTGA	3 cut(s)	425, 953, 986
AvaI	CYCGRG	1 cut(s)	63
AvaII	GGWCC	2 cut(s)	449, 1151
BaeGI	GKGCMC	1 cut(s)	899
BalI	TGGCCA	1 cut(s)	622
BbsI	GAAGAC	3 cut(s)	134, 406, 503
BbvI	GCAGC	3 cut(s)	715, 913, 1195
BccI	CCATC	5 cut(s)	37, 41, 659, 818, 896
BceAI	ACGGC	1 cut(s)	1187
BciT130I	CCWGG	2 cut(s)	372, 453
BclI	TGATCA	1 cut(s)	415
BcoDI	GTCTC	1 cut(s)	758
BfaI	CTAG	1 cut(s)	1164
BfmI	CTRYAG	1 cut(s)	1209
BfrI	CTTAAG	1 cut(s)	653
BisI	GCNGC	3 cut(s)	729, 927, 1209
BlsI	GCNGC	3 cut(s)	730, 928, 1210
BmcAI	AGTACT	2 cut(s)	128, 888
Bme1390I	CCNGG	2 cut(s)	372, 453
Bme18I	GGWCC	2 cut(s)	449, 1151
BmeT110I	CYCGRG	1 cut(s)	63
BmgT120I	GGNCC	2 cut(s)	449, 1151
BmiI	GGNNCC	1 cut(s)	1169
BmrFI	CCNGG	2 cut(s)	372, 453
BmrI	ACTGGG	1 cut(s)	700
BmsI	GCATC	3 cut(s)	145, 232, 1189
BmuI	ACTGGG	1 cut(s)	700
BoxI	GACNNNNGTC	1 cut(s)	138
BpiI	GAAGAC	3 cut(s)	134, 406, 503
Bpu10I	CCTNAGC	1 cut(s)	1155
BpuEI	CTTGAG	1 cut(s)	837
Bsa29I	ATCGAT	1 cut(s)	424
BsaBI	GATNNNNATC	2 cut(s)	246, 420
BsaJI	CCNNGG	5 cut(s)	136, 370, 452, 1125, 1203
Bsc4I	CCNNNNNNNGG	1 cut(s)	109
Bse1I	ACTGG	3 cut(s)	510, 706, 894
Bse8I	GATNNNNATC	2 cut(s)	246, 420
BseBI	CCWGG	2 cut(s)	372, 453
BseCI	ATCGAT	1 cut(s)	424
BseDI	CCNNGG	5 cut(s)	136, 370, 452, 1125, 1203
BseGI	GGATG	3 cut(s)	496, 514, 1051
BseJI	GATNNNNATC	2 cut(s)	246, 420
BseLI	CCNNNNNNNGG	1 cut(s)	109
BseMII	CTCAG	1 cut(s)	1088
BseNI	ACTGG	3 cut(s)	510, 706, 894
BseRI	GAGGAG	2 cut(s)	298, 1202
BseSI	GKGCMC	1 cut(s)	899
BseXI	GCAGC	3 cut(s)	715, 913, 1195
BsgI	GTGCAG	1 cut(s)	207
BshFI	GGCC	1 cut(s)	622
BshVI	ATCGAT	1 cut(s)	424
BsiHKCI	CYCGRG	1 cut(s)	63
BslFI	GGGAC	1 cut(s)	273
BslI	CCNNNNNNNGG	1 cut(s)	109
BsmAI	GTCTC	1 cut(s)	758
BsmBI	CGTCTC	1 cut(s)	758
BsmFI	GGGAC	1 cut(s)	273
BsnI	GGCC	1 cut(s)	622
BsoBI	CYCGRG	1 cut(s)	63
Bsp1286I	GDGCHC	1 cut(s)	899
Bsp143I	GATC	6 cut(s)	15, 112, 415, 425, 863, 1129
BspANI	GGCC	1 cut(s)	622
BspCNI	CTCAG	1 cut(s)	1087
BspDI	ATCGAT	1 cut(s)	424
BspLI	GGNNCC	1 cut(s)	1169
BspMAI	CTGCAG	1 cut(s)	1213
BspPI	GGATC	3 cut(s)	120, 871, 1137
BspTI	CTTAAG	1 cut(s)	653
BsrI	ACTGG	3 cut(s)	510, 706, 894
BssECI	CCNNGG	5 cut(s)	136, 370, 452, 1125, 1203
BssMI	GATC	6 cut(s)	15, 112, 415, 425, 863, 1129
BssNAI	GTATAC	1 cut(s)	514
BssT1I	CCWWGG	2 cut(s)	136, 1125
Bst1107I	GTATAC	1 cut(s)	514
Bst2UI	CCWGG	2 cut(s)	372, 453
Bst4CI	ACNGT	7 cut(s)	178, 232, 279, 325, 649, 940, 1105
BstAFI	CTTAAG	1 cut(s)	653
BstC8I	GCNNGC	4 cut(s)	733, 811, 1026, 1161
BstDEI	CTNAG	6 cut(s)	200, 632, 717, 847, 1074, 1155
BstDSI	CCRYGG	1 cut(s)	1203
BstF5I	GGATG	3 cut(s)	496, 514, 1051
BstHHI	GCGC	1 cut(s)	227
BstKTI	GATC	6 cut(s)	18, 115, 418, 428, 866, 1132
BstMAI	GTCTC	1 cut(s)	758
BstMBI	GATC	6 cut(s)	15, 112, 415, 425, 863, 1129
BstMWI	GCNNNNNNNGC	4 cut(s)	926, 990, 1034, 1208
BstNI	CCWGG	2 cut(s)	372, 453
BstNSI	RCATGY	2 cut(s)	407, 813
BstPAI	GACNNNNGTC	1 cut(s)	138
BstSCI	CCNGG	2 cut(s)	370, 451
BstSFI	CTRYAG	1 cut(s)	1209
BstSLI	GKGCMC	1 cut(s)	899
BstV1I	GCAGC	3 cut(s)	715, 913, 1195
BstV2I	GAAGAC	3 cut(s)	134, 406, 503
BstX2I	RGATCY	3 cut(s)	112, 863, 1129
BstXI	CCANNNNNNTGG	1 cut(s)	971
BstYI	RGATCY	3 cut(s)	112, 863, 1129
BstZ17I	GTATAC	1 cut(s)	514
Bsu15I	ATCGAT	1 cut(s)	424
BsuRI	GGCC	1 cut(s)	622
BsuTUI	ATCGAT	1 cut(s)	424
BtgI	CCRYGG	1 cut(s)	1203
BtsCI	GGATG	3 cut(s)	496, 514, 1051
BtsIMutI	CAGTG	2 cut(s)	228, 713
Cac8I	GCNNGC	4 cut(s)	733, 811, 1026, 1161
CaiI	CAGNNNCTG	1 cut(s)	1081
CfoI	GCGC	1 cut(s)	227
Cfr13I	GGNCC	2 cut(s)	449, 1151
ClaI	ATCGAT	1 cut(s)	424
Csp6I	GTAC	6 cut(s)	127, 205, 321, 661, 887, 936
CviAII	CATG	6 cut(s)	253, 404, 550, 810, 932, 1148
CviQI	GTAC	6 cut(s)	127, 205, 321, 661, 887, 936
DdeI	CTNAG	6 cut(s)	200, 632, 717, 847, 1074, 1155
DpnI	GATC	6 cut(s)	17, 114, 417, 427, 865, 1131
DpnII	GATC	6 cut(s)	15, 112, 415, 425, 863, 1129
EaeI	YGGCCR	1 cut(s)	620
Eco130I	CCWWGG	2 cut(s)	136, 1125
Eco47I	GGWCC	2 cut(s)	449, 1151
Eco57I	CTGAAG	1 cut(s)	942
Eco88I	CYCGRG	1 cut(s)	63
EcoO109I	RGGNCCY	1 cut(s)	449
EcoRII	CCWGG	2 cut(s)	370, 451
EcoT14I	CCWWGG	2 cut(s)	136, 1125
EcoT22I	ATGCAT	1 cut(s)	395
ErhI	CCWWGG	2 cut(s)	136, 1125
Esp3I	CGTCTC	1 cut(s)	758
FaeI	CATG	6 cut(s)	256, 407, 553, 813, 935, 1151
FalI	AAGNNNNNCTT	3 cut(s)	391, 423, 1212
FaqI	GGGAC	1 cut(s)	273
FatI	CATG	6 cut(s)	252, 403, 549, 809, 931, 1147
FauNDI	CATATG	1 cut(s)	737
FbaI	TGATCA	1 cut(s)	415
FblI	GTMKAC	1 cut(s)	513
Fnu4HI	GCNGC	3 cut(s)	729, 927, 1209
FokI	GGATG	3 cut(s)	503, 521, 1058
Fsp4HI	GCNGC	3 cut(s)	729, 927, 1209
FspBI	CTAG	1 cut(s)	1164
GlaI	GCGC	1 cut(s)	226
GluI	GCNGC	3 cut(s)	729, 927, 1209
HaeIII	GGCC	1 cut(s)	622
HhaI	GCGC	1 cut(s)	227
Hin1II	CATG	6 cut(s)	256, 407, 553, 813, 935, 1151
Hin6I	GCGC	1 cut(s)	225
HinP1I	GCGC	1 cut(s)	225
HindIII	AAGCTT	4 cut(s)	92, 1089, 1157, 1226
HinfI	GANTC	6 cut(s)	61, 286, 340, 566, 672, 1109
HphI	GGTGA	3 cut(s)	425, 953, 986
Hpy166II	GTNNAC	2 cut(s)	514, 961
Hpy188I	TCNGA	2 cut(s)	332, 1077
Hpy188III	TCNNGA	7 cut(s)	10, 73, 116, 152, 479, 689, 1233
Hpy8I	GTNNAC	2 cut(s)	514, 961
HpyAV	CCTTC	4 cut(s)	250, 476, 524, 785
HpyCH4III	ACNGT	7 cut(s)	178, 232, 279, 325, 649, 940, 1105
HpyCH4IV	ACGT	2 cut(s)	354, 558
HpyF10VI	GCNNNNNNNGC	4 cut(s)	926, 990, 1034, 1208
HpyF3I	CTNAG	6 cut(s)	200, 632, 717, 847, 1074, 1155
HpySE526I	ACGT	2 cut(s)	354, 558
Hsp92II	CATG	6 cut(s)	256, 407, 553, 813, 935, 1151
HspAI	GCGC	1 cut(s)	225
Ksp22I	TGATCA	1 cut(s)	415
Kzo9I	GATC	6 cut(s)	15, 112, 415, 425, 863, 1129
Lsp1109I	GCAGC	3 cut(s)	715, 913, 1195
LweI	GCATC	3 cut(s)	145, 232, 1189
MaeI	CTAG	1 cut(s)	1164
MaeII	ACGT	2 cut(s)	354, 558
MaeIII	GTNAC	3 cut(s)	172, 295, 940
MalI	GATC	6 cut(s)	17, 114, 417, 427, 865, 1131
MboI	GATC	6 cut(s)	15, 112, 415, 425, 863, 1129
MboII	GAAGA	7 cut(s)	25, 134, 411, 508, 681, 1097, 1136
MfeI	CAATTG	1 cut(s)	429
MflI	RGATCY	3 cut(s)	112, 863, 1129
MhlI	GDGCHC	1 cut(s)	899
MlsI	TGGCCA	1 cut(s)	622
MluCI	AATT	7 cut(s)	81, 429, 464, 817, 1002, 1058, 1140
MluNI	TGGCCA	1 cut(s)	622
MlyI	GAGTC	3 cut(s)	55, 295, 575
MmeI	TCCRAC	2 cut(s)	1023, 1044
MnlI	CCTC	7 cut(s)	156, 184, 276, 369, 580, 1013, 1180
Mox20I	TGGCCA	1 cut(s)	622
Mph1103I	ATGCAT	1 cut(s)	395
MroXI	GAANNNNTTC	1 cut(s)	339
MscI	TGGCCA	1 cut(s)	622
MseI	TTAA	3 cut(s)	84, 654, 783
MslI	CAYNNNNRTG	1 cut(s)	153
Msp20I	TGGCCA	1 cut(s)	622
MspCI	CTTAAG	1 cut(s)	653
MspR9I	CCNGG	2 cut(s)	372, 453
MunI	CAATTG	1 cut(s)	429
MvaI	CCWGG	2 cut(s)	372, 453
MwoI	GCNNNNNNNGC	4 cut(s)	926, 990, 1034, 1208
NdeI	CATATG	1 cut(s)	737
NdeII	GATC	6 cut(s)	15, 112, 415, 425, 863, 1129
NlaIII	CATG	6 cut(s)	256, 407, 553, 813, 935, 1151
NlaIV	GGNNCC	1 cut(s)	1169
NmeAIII	GCCGAG	1 cut(s)	925
NmuCI	GTSAC	1 cut(s)	295
NsiI	ATGCAT	1 cut(s)	395
NspI	RCATGY	2 cut(s)	407, 813
PaeI	GCATGC	1 cut(s)	813
PaeR7I	CTCGAG	1 cut(s)	63
PdmI	GAANNNNTTC	1 cut(s)	339
PfeI	GAWTC	3 cut(s)	340, 672, 1109
PkrI	GCNGC	3 cut(s)	730, 928, 1210
PleI	GAGTC	3 cut(s)	55, 294, 574
PpsI	GAGTC	3 cut(s)	55, 294, 574
PpuMI	RGGWCCY	1 cut(s)	449
PshAI	GACNNNNGTC	1 cut(s)	138
Psp1406I	AACGTT	2 cut(s)	354, 558
Psp5II	RGGWCCY	1 cut(s)	449
Psp6I	CCWGG	2 cut(s)	370, 451
PspGI	CCWGG	2 cut(s)	370, 451
PspN4I	GGNNCC	1 cut(s)	1169
PspPI	GGNCC	2 cut(s)	449, 1151
PspPPI	RGGWCCY	1 cut(s)	449
PspXI	VCTCGAGB	1 cut(s)	63
PstI	CTGCAG	1 cut(s)	1213
PstNI	CAGNNNCTG	1 cut(s)	1081
PsuI	RGATCY	3 cut(s)	112, 863, 1129
RsaI	GTAC	6 cut(s)	128, 206, 322, 662, 888, 937
RsaNI	GTAC	6 cut(s)	127, 205, 321, 661, 887, 936
RseI	CAYNNNNRTG	1 cut(s)	153
SaqAI	TTAA	3 cut(s)	84, 654, 783
SatI	GCNGC	3 cut(s)	729, 927, 1209
Sau3AI	GATC	6 cut(s)	15, 112, 415, 425, 863, 1129
Sau96I	GGNCC	2 cut(s)	449, 1151
ScaI	AGTACT	2 cut(s)	128, 888
SchI	GAGTC	3 cut(s)	55, 295, 575
ScrFI	CCNGG	2 cut(s)	372, 453
SduI	GDGCHC	1 cut(s)	899
SfaNI	GCATC	3 cut(s)	145, 232, 1189
SfcI	CTRYAG	1 cut(s)	1209
Sfr274I	CTCGAG	1 cut(s)	63
SinI	GGWCC	2 cut(s)	449, 1151
SlaI	CTCGAG	1 cut(s)	63
SmiMI	CAYNNNNRTG	1 cut(s)	153
SmlI	CTYRAG	3 cut(s)	63, 653, 852
SmoI	CTYRAG	3 cut(s)	63, 653, 852
SphI	GCATGC	1 cut(s)	813
Sse9I	AATT	7 cut(s)	81, 429, 464, 817, 1002, 1058, 1140
SspMI	CTAG	1 cut(s)	1164
StyD4I	CCNGG	2 cut(s)	370, 451
StyI	CCWWGG	2 cut(s)	136, 1125
TaaI	ACNGT	7 cut(s)	178, 232, 279, 325, 649, 940, 1105
TaiI	ACGT	2 cut(s)	357, 561
TaqI	TCGA	4 cut(s)	9, 64, 236, 424
TasI	AATT	7 cut(s)	81, 429, 464, 817, 1002, 1058, 1140
TatI	WGTACW	2 cut(s)	126, 886
TfiI	GAWTC	3 cut(s)	340, 672, 1109
Tru1I	TTAA	3 cut(s)	84, 654, 783
Tru9I	TTAA	3 cut(s)	84, 654, 783
TscAI	CASTG	2 cut(s)	235, 713
TseFI	GTSAC	1 cut(s)	295
TseI	GCWGC	3 cut(s)	728, 926, 1208
Tsp45I	GTSAC	1 cut(s)	295
TspDTI	ATGAA	6 cut(s)	269, 714, 738, 759, 1136, 1183
TspGWI	ACGGA	3 cut(s)	63, 563, 1199
TspRI	CASTG	2 cut(s)	235, 713
Vha464I	CTTAAG	1 cut(s)	653
VpaK11BI	GGWCC	2 cut(s)	449, 1151
XceI	RCATGY	2 cut(s)	407, 813
XcmI	CCANNNNNNNNNTGG	1 cut(s)	40
XhoI	CTCGAG	1 cut(s)	63
XmiI	GTMKAC	1 cut(s)	513
XmnI	GAANNNNTTC	1 cut(s)	339
XspI	CTAG	1 cut(s)	1164
ZrmI	AGTACT	2 cut(s)	128, 888
Zsp2I	ATGCAT	1 cut(s)	395

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.