Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0002227.1

Orthogroup ID

OG0005226

Physical Location & Seq

Genomic Coordinates Reverse (-)

28459 .. 32597

Gene Structure 4139 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0002227.1_g000003.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1617 bp

atggcgttcgagaagatcaaggtcgctaaccccatcgtcgagatggacggagatgagatgactcgaattttctggaaatcaatcaaggataagcttattctcccctttttggatttggacattaagtactttgaccttggtcttcctcaccgtgatgccactgatgataaagttaccgttgaaagtgcagaggctactctcaagtacaatgtagcaatcaaatgcgcgactatcacaccagatgaagctcgcatgaaggagtttagcttgaagcaaatgtggaggagtcccaatgggactattaggaatattttgaatggtactgttttcagagaaccgattctttgcaagaacattcctcgcctaatcccaggttggacgaagccaatatgcattggaagacatgctttcggtgatcaatatcgagcaactgatacagttatcaaaggacctgggaaactgaaattgctgtttgttccagaaggaaaggatgagaagactgaactggaagtgtacaactttacaggggaaggcggagtagcaattgccatgtacaacaccgatgagtcaatccgtgcttttgcagaagcttctatgaacacagcttatgagaaaaagtggcctctttatcttagcacaaaaaacactattcttaagaagtatgacggaagattcaaggacatatttcaggaagtttacgaagctcattggaaatccaagtatgaagctgctggcatatggtatgaacatcgtctcattgatgatatggtggcttatgcacttaaaagtgaaggtggttatgtttgggcatgcaagaattatgatggagatgtgcaaagtgatttcttagctcaagttccagaaggaaaggatgagaagactgaactggaagtgtacaactttacaggggaaggcggagtagcaattgccatgtacaacaccgatgagtcaatccgtgcttttgcagaagcttctatgaacacagcttatgagaaaaagtggcctctttatcttagcacaaaaaacactattcttaagaagtatgacggaagattcaaggacatatttcaggaagtttacgaagctcattggaaatccaagtatgaagctgctggcatatggtatgaacatcgtctcattgatgatatggtggcttatgcacttaaaagtgaaggtggttatgtttgggcatgcaagaattatgatggagatgtgcaaagtgatttcttagctcaaggttttggatctcttggattgatgacatcagtactggtgtgcccagatggaaagaccatagaagctgaagctgctcatggtacagttactcggcattatagagttcaccagaaaggtggtgaaaccagcacaaacagcatagcttcaatttttgcttggacgagagggcttgcacacagggcaaagttggatgacaatgctaaattgttggatttcactcagaagctggaagaagcttgtattggaactgtggaatctgggaaaatgaccaaggatcttgcactaattcttcatggacctaagcttgctaggaaccactacttgaacacagaggagttcattgatgccgtggctgcagaactgaaagcaaagctttcttga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004448 GO:0004450 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006102 GO:0006139 GO:0006725 GO:0006732 GO:0006733 GO:0006739 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009117 GO:0009506 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009651 GO:0009987 GO:0010035 GO:0010038 GO:0010043 GO:0016020 GO:0016491 GO:0016614 GO:0016616 GO:0019362 GO:0019637 GO:0019752 GO:0030054 GO:0034641 GO:0042221 GO:0042742 GO:0043167 GO:0043169 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046496 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050896 GO:0051186 GO:0051704 GO:0051707 GO:0055044 GO:0055086 GO:0055114 GO:0071704 GO:0071944 GO:0072350 GO:0072524 GO:0098542 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00020 ko00480 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 ko04146 map00020 map00480 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230 map04146

Pfam Domains

Protein Families

Iso_dh

Protein Analysis

538

Amino Acids

60.94

Weight (kDa)

5.85

Isoelectric Point (pI)

20.08

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005226)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65930
fragaria_vesca	FvH4_1g27490 FvH4_1g27490 FvH4_1g30530 FvH4_1g30530
malus_domestica	MD09G1276900.v1.1 MD09G1277300.v1.1 MD17G1270700.v1.1
prunus_persica	Prupe.3G015500_v2.0.a1 Prupe.3G015500_v2.0.a1 Prupe.3G015500_v2.0.a1 Prupe.3G015500_v2.0.a1
pyrus_communis	pycom09g18670 pycom17g26910
rosa_chinensis	RchiOBHm_Chr3g0489041 RchiOBHm_Chr3g0489051
rosa_laevigata	RLG00000022897 RLG00000022898
rosa_multiflora	Rmu_sc0002227.1_g000003 Rmu_sc0011911.1_g000008
rosa_roxburghii	Rroxscaffold_6G00393730
rosa_rugosa	Rorug03G0242800 Rorug03G0242900 Rorug03G0242900
rosa_samantha	Rh3AG291100 Rh3BG327800 Rh3DG324200
rosa_wichuraiana	Rw3G025760 Rw3G025770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	227
AciI	CCGC	2 cut(s)	534, 915
AclWI	GGATC	2 cut(s)	1252, 1518
AcsI	RAATTY	1 cut(s)	66
AcuI	CTGAAG	1 cut(s)	1323
AfaI	GTAC	9 cut(s)	128, 206, 322, 515, 554, 896, 935, 1269, 1318
AfiI	CCNNNNNNNGG	1 cut(s)	109
AflII	CTTAAG	2 cut(s)	653, 1034
AgsI	TTSAA	7 cut(s)	182, 271, 316, 676, 1057, 1383, 1561
AjnI	CCWGG	2 cut(s)	370, 451
AjuI	GAANNNNNNNTTGG	2 cut(s)	1461, 1493
Alw26I	GTCTC	2 cut(s)	758, 1139
AlwI	GGATC	2 cut(s)	1252, 1518
AlwNI	CAGNNNCTG	1 cut(s)	1462
AoxI	GGCC	2 cut(s)	620, 1001
ApeKI	GCWGC	4 cut(s)	728, 1109, 1307, 1589
ApoI	RAATTY	1 cut(s)	66
Asp700I	GAANNNNTTC	1 cut(s)	339
AspLEI	GCGC	1 cut(s)	227
AspS9I	GGNCC	2 cut(s)	449, 1532
AsuHPI	GGTGA	4 cut(s)	140, 425, 1334, 1367
AvaII	GGWCC	2 cut(s)	449, 1532
BaeGI	GKGCMC	1 cut(s)	1280
BbsI	GAAGAC	4 cut(s)	134, 406, 503, 884
BbvI	GCAGC	4 cut(s)	715, 1096, 1294, 1576
BccI	CCATC	5 cut(s)	37, 41, 818, 1199, 1277
BceAI	ACGGC	1 cut(s)	1568
BciT130I	CCWGG	2 cut(s)	372, 453
BclI	TGATCA	1 cut(s)	415
BcoDI	GTCTC	2 cut(s)	758, 1139
BfaI	CTAG	1 cut(s)	1545
BfmI	CTRYAG	1 cut(s)	1590
BfrI	CTTAAG	2 cut(s)	653, 1034
BisI	GCNGC	4 cut(s)	729, 1110, 1308, 1590
BlsI	GCNGC	4 cut(s)	730, 1111, 1309, 1591
BmcAI	AGTACT	2 cut(s)	128, 1269
Bme1390I	CCNGG	2 cut(s)	372, 453
Bme18I	GGWCC	2 cut(s)	449, 1532
BmgT120I	GGNCC	2 cut(s)	449, 1532
BmiI	GGNNCC	1 cut(s)	1550
BmrFI	CCNGG	2 cut(s)	372, 453
BmsI	GCATC	2 cut(s)	145, 1570
BoxI	GACNNNNGTC	1 cut(s)	138
BpiI	GAAGAC	4 cut(s)	134, 406, 503, 884
Bpu10I	CCTNAGC	1 cut(s)	1536
BpuEI	CTTGAG	3 cut(s)	185, 837, 1218
BsaBI	GATNNNNATC	1 cut(s)	420
BsaJI	CCNNGG	5 cut(s)	136, 370, 452, 1506, 1584
Bsc4I	CCNNNNNNNGG	1 cut(s)	109
Bse1I	ACTGG	3 cut(s)	510, 891, 1275
Bse8I	GATNNNNATC	1 cut(s)	420
BseBI	CCWGG	2 cut(s)	372, 453
BseDI	CCNNGG	5 cut(s)	136, 370, 452, 1506, 1584
BseGI	GGATG	3 cut(s)	496, 877, 1432
BseJI	GATNNNNATC	1 cut(s)	420
BseLI	CCNNNNNNNGG	1 cut(s)	109
BseMII	CTCAG	1 cut(s)	1469
BseNI	ACTGG	3 cut(s)	510, 891, 1275
BseRI	GAGGAG	2 cut(s)	298, 1583
BseSI	GKGCMC	1 cut(s)	1280
BseXI	GCAGC	4 cut(s)	715, 1096, 1294, 1576
BsgI	GTGCAG	1 cut(s)	207
Bsh1236I	CGCG	1 cut(s)	227
BshFI	GGCC	2 cut(s)	622, 1003
BslFI	GGGAC	2 cut(s)	273, 310
BslI	CCNNNNNNNGG	1 cut(s)	109
BsmAI	GTCTC	2 cut(s)	758, 1139
BsmBI	CGTCTC	2 cut(s)	758, 1139
BsmFI	GGGAC	2 cut(s)	273, 310
BsnI	GGCC	2 cut(s)	622, 1003
Bsp1286I	GDGCHC	1 cut(s)	1280
Bsp1407I	TGTACA	4 cut(s)	513, 552, 894, 933
Bsp143I	GATC	4 cut(s)	15, 415, 1244, 1510
BspACI	CCGC	2 cut(s)	534, 915
BspANI	GGCC	2 cut(s)	622, 1003
BspCNI	CTCAG	1 cut(s)	1468
BspFNI	CGCG	1 cut(s)	227
BspLI	GGNNCC	1 cut(s)	1550
BspMAI	CTGCAG	1 cut(s)	1594
BspPI	GGATC	2 cut(s)	1252, 1518
BspTI	CTTAAG	2 cut(s)	653, 1034
BsrGI	TGTACA	4 cut(s)	513, 552, 894, 933
BsrI	ACTGG	3 cut(s)	510, 891, 1275
BssECI	CCNNGG	5 cut(s)	136, 370, 452, 1506, 1584
BssMI	GATC	4 cut(s)	15, 415, 1244, 1510
BssT1I	CCWWGG	2 cut(s)	136, 1506
Bst2UI	CCWGG	2 cut(s)	372, 453
Bst4CI	ACNGT	6 cut(s)	152, 178, 325, 439, 1321, 1486
BstAFI	CTTAAG	2 cut(s)	653, 1034
BstAUI	TGTACA	4 cut(s)	513, 552, 894, 933
BstC8I	GCNNGC	7 cut(s)	250, 733, 811, 1114, 1192, 1407, 1542
BstDEI	CTNAG	6 cut(s)	632, 847, 1013, 1228, 1455, 1536
BstDSI	CCRYGG	1 cut(s)	1584
BstF5I	GGATG	3 cut(s)	496, 877, 1432
BstFNI	CGCG	1 cut(s)	227
BstHHI	GCGC	1 cut(s)	227
BstKTI	GATC	4 cut(s)	18, 418, 1247, 1513
BstMAI	GTCTC	2 cut(s)	758, 1139
BstMBI	GATC	4 cut(s)	15, 415, 1244, 1510
BstMWI	GCNNNNNNNGC	4 cut(s)	1307, 1371, 1415, 1589
BstNI	CCWGG	2 cut(s)	372, 453
BstNSI	RCATGY	3 cut(s)	407, 813, 1194
BstPAI	GACNNNNGTC	1 cut(s)	138
BstSCI	CCNGG	2 cut(s)	370, 451
BstSFI	CTRYAG	1 cut(s)	1590
BstSLI	GKGCMC	1 cut(s)	1280
BstUI	CGCG	1 cut(s)	227
BstV1I	GCAGC	4 cut(s)	715, 1096, 1294, 1576
BstV2I	GAAGAC	4 cut(s)	134, 406, 503, 884
BstX2I	RGATCY	2 cut(s)	1244, 1510
BstXI	CCANNNNNNTGG	1 cut(s)	1352
BstYI	RGATCY	2 cut(s)	1244, 1510
BsuRI	GGCC	2 cut(s)	622, 1003
BtgI	CCRYGG	1 cut(s)	1584
BtsCI	GGATG	3 cut(s)	496, 877, 1432
BtsIMutI	CAGTG	1 cut(s)	159
Cac8I	GCNNGC	7 cut(s)	250, 733, 811, 1114, 1192, 1407, 1542
CaiI	CAGNNNCTG	1 cut(s)	1462
CfoI	GCGC	1 cut(s)	227
Cfr13I	GGNCC	2 cut(s)	449, 1532
Csp6I	GTAC	9 cut(s)	127, 205, 321, 514, 553, 895, 934, 1268, 1317
CviAII	CATG	8 cut(s)	253, 404, 550, 810, 931, 1191, 1313, 1529
CviQI	GTAC	9 cut(s)	127, 205, 321, 514, 553, 895, 934, 1268, 1317
DdeI	CTNAG	6 cut(s)	632, 847, 1013, 1228, 1455, 1536
DpnI	GATC	4 cut(s)	17, 417, 1246, 1512
DpnII	GATC	4 cut(s)	15, 415, 1244, 1510
EciI	GGCGGA	2 cut(s)	549, 930
Eco130I	CCWWGG	2 cut(s)	136, 1506
Eco47I	GGWCC	2 cut(s)	449, 1532
Eco57I	CTGAAG	1 cut(s)	1323
EcoO109I	RGGNCCY	1 cut(s)	449
EcoRII	CCWGG	2 cut(s)	370, 451
EcoT14I	CCWWGG	2 cut(s)	136, 1506
EcoT22I	ATGCAT	1 cut(s)	395
ErhI	CCWWGG	2 cut(s)	136, 1506
Esp3I	CGTCTC	2 cut(s)	758, 1139
FaeI	CATG	8 cut(s)	256, 407, 553, 813, 934, 1194, 1316, 1532
FalI	AAGNNNNNCTT	3 cut(s)	391, 423, 1593
FaqI	GGGAC	2 cut(s)	273, 310
FatI	CATG	8 cut(s)	252, 403, 549, 809, 930, 1190, 1312, 1528
FauNDI	CATATG	2 cut(s)	737, 1118
FbaI	TGATCA	1 cut(s)	415
Fnu4HI	GCNGC	4 cut(s)	729, 1110, 1308, 1590
FokI	GGATG	3 cut(s)	503, 884, 1439
Fsp4HI	GCNGC	4 cut(s)	729, 1110, 1308, 1590
FspBI	CTAG	1 cut(s)	1545
GlaI	GCGC	1 cut(s)	226
GluI	GCNGC	4 cut(s)	729, 1110, 1308, 1590
HaeIII	GGCC	2 cut(s)	622, 1003
HhaI	GCGC	1 cut(s)	227
Hin1II	CATG	8 cut(s)	256, 407, 553, 813, 934, 1194, 1316, 1532
Hin6I	GCGC	1 cut(s)	225
HinP1I	GCGC	1 cut(s)	225
HindIII	AAGCTT	6 cut(s)	92, 588, 969, 1470, 1538, 1607
HinfI	GANTC	8 cut(s)	61, 286, 340, 566, 672, 947, 1053, 1490
HphI	GGTGA	4 cut(s)	140, 425, 1334, 1367
Hpy166II	GTNNAC	5 cut(s)	514, 697, 895, 1078, 1342
Hpy188I	TCNGA	2 cut(s)	332, 1458
Hpy188III	TCNNGA	8 cut(s)	10, 40, 73, 479, 689, 860, 1070, 1614
Hpy8I	GTNNAC	5 cut(s)	514, 697, 895, 1078, 1342
Hpy99I	CGWCG	1 cut(s)	41
HpyAV	CCTTC	7 cut(s)	250, 476, 524, 785, 857, 905, 1166
HpyCH4III	ACNGT	6 cut(s)	152, 178, 325, 439, 1321, 1486
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1307, 1371, 1415, 1589
HpyF3I	CTNAG	6 cut(s)	632, 847, 1013, 1228, 1455, 1536
Hsp92II	CATG	8 cut(s)	256, 407, 553, 813, 934, 1194, 1316, 1532
HspAI	GCGC	1 cut(s)	225
Ksp22I	TGATCA	1 cut(s)	415
Kzo9I	GATC	4 cut(s)	15, 415, 1244, 1510
Lsp1109I	GCAGC	4 cut(s)	715, 1096, 1294, 1576
LweI	GCATC	2 cut(s)	145, 1570
MaeI	CTAG	1 cut(s)	1545
MaeIII	GTNAC	2 cut(s)	172, 1321
MalI	GATC	4 cut(s)	17, 417, 1246, 1512
MboI	GATC	4 cut(s)	15, 415, 1244, 1510
MboII	GAAGA	9 cut(s)	25, 134, 411, 508, 681, 889, 1062, 1478, 1517
MfeI	CAATTG	2 cut(s)	543, 924
MflI	RGATCY	2 cut(s)	1244, 1510
MhlI	GDGCHC	1 cut(s)	1280
MluCI	AATT	9 cut(s)	66, 464, 543, 817, 924, 1198, 1383, 1439, 1521
MlyI	GAGTC	4 cut(s)	55, 295, 575, 956
MmeI	TCCRAC	3 cut(s)	356, 1404, 1425
MnlI	CCTC	8 cut(s)	156, 184, 276, 369, 633, 1014, 1394, 1561
Mph1103I	ATGCAT	1 cut(s)	395
MroXI	GAANNNNTTC	1 cut(s)	339
MseI	TTAA	5 cut(s)	123, 654, 783, 1035, 1164
MslI	CAYNNNNRTG	1 cut(s)	153
MspCI	CTTAAG	2 cut(s)	653, 1034
MspR9I	CCNGG	2 cut(s)	372, 453
MunI	CAATTG	2 cut(s)	543, 924
MvaI	CCWGG	2 cut(s)	372, 453
MvnI	CGCG	1 cut(s)	227
MwoI	GCNNNNNNNGC	4 cut(s)	1307, 1371, 1415, 1589
NdeI	CATATG	2 cut(s)	737, 1118
NdeII	GATC	4 cut(s)	15, 415, 1244, 1510
NlaIII	CATG	8 cut(s)	256, 407, 553, 813, 934, 1194, 1316, 1532
NlaIV	GGNNCC	1 cut(s)	1550
NmeAIII	GCCGAG	1 cut(s)	1306
NsiI	ATGCAT	1 cut(s)	395
NspI	RCATGY	3 cut(s)	407, 813, 1194
PaeI	GCATGC	2 cut(s)	813, 1194
PdmI	GAANNNNTTC	1 cut(s)	339
PfeI	GAWTC	4 cut(s)	340, 672, 1053, 1490
PkrI	GCNGC	4 cut(s)	730, 1111, 1309, 1591
PleI	GAGTC	4 cut(s)	55, 294, 574, 955
PpsI	GAGTC	4 cut(s)	55, 294, 574, 955
PpuMI	RGGWCCY	1 cut(s)	449
PshAI	GACNNNNGTC	1 cut(s)	138
Psp5II	RGGWCCY	1 cut(s)	449
Psp6I	CCWGG	2 cut(s)	370, 451
PspGI	CCWGG	2 cut(s)	370, 451
PspN4I	GGNNCC	1 cut(s)	1550
PspPI	GGNCC	2 cut(s)	449, 1532
PspPPI	RGGWCCY	1 cut(s)	449
PstI	CTGCAG	1 cut(s)	1594
PstNI	CAGNNNCTG	1 cut(s)	1462
PsuI	RGATCY	2 cut(s)	1244, 1510
RsaI	GTAC	9 cut(s)	128, 206, 322, 515, 554, 896, 935, 1269, 1318
RsaNI	GTAC	9 cut(s)	127, 205, 321, 514, 553, 895, 934, 1268, 1317
RseI	CAYNNNNRTG	1 cut(s)	153
SaqAI	TTAA	5 cut(s)	123, 654, 783, 1035, 1164
SatI	GCNGC	4 cut(s)	729, 1110, 1308, 1590
Sau3AI	GATC	4 cut(s)	15, 415, 1244, 1510
Sau96I	GGNCC	2 cut(s)	449, 1532
ScaI	AGTACT	2 cut(s)	128, 1269
SchI	GAGTC	4 cut(s)	55, 295, 575, 956
ScrFI	CCNGG	2 cut(s)	372, 453
SduI	GDGCHC	1 cut(s)	1280
SfaNI	GCATC	2 cut(s)	145, 1570
SfcI	CTRYAG	1 cut(s)	1590
SinI	GGWCC	2 cut(s)	449, 1532
SmiMI	CAYNNNNRTG	1 cut(s)	153
SmlI	CTYRAG	5 cut(s)	200, 653, 852, 1034, 1233
SmoI	CTYRAG	5 cut(s)	200, 653, 852, 1034, 1233
SphI	GCATGC	2 cut(s)	813, 1194
Sse9I	AATT	9 cut(s)	66, 464, 543, 817, 924, 1198, 1383, 1439, 1521
SsiI	CCGC	2 cut(s)	534, 915
SspI	AATATT	1 cut(s)	310
SspMI	CTAG	1 cut(s)	1545
StyD4I	CCNGG	2 cut(s)	370, 451
StyI	CCWWGG	2 cut(s)	136, 1506
TaaI	ACNGT	6 cut(s)	152, 178, 325, 439, 1321, 1486
TaqI	TCGA	4 cut(s)	9, 39, 64, 424
TasI	AATT	9 cut(s)	66, 464, 543, 817, 924, 1198, 1383, 1439, 1521
TatI	WGTACW	7 cut(s)	126, 204, 513, 552, 894, 933, 1267
TfiI	GAWTC	4 cut(s)	340, 672, 1053, 1490
Tru1I	TTAA	5 cut(s)	123, 654, 783, 1035, 1164
Tru9I	TTAA	5 cut(s)	123, 654, 783, 1035, 1164
TscAI	CASTG	1 cut(s)	166
TseI	GCWGC	4 cut(s)	728, 1109, 1307, 1589
TspGWI	ACGGA	5 cut(s)	63, 563, 681, 944, 1062
TspRI	CASTG	1 cut(s)	166
Vha464I	CTTAAG	2 cut(s)	653, 1034
VpaK11BI	GGWCC	2 cut(s)	449, 1532
XapI	RAATTY	1 cut(s)	66
XceI	RCATGY	3 cut(s)	407, 813, 1194
XcmI	CCANNNNNNNNNTGG	1 cut(s)	40
XmnI	GAANNNNTTC	1 cut(s)	339
XspI	CTAG	1 cut(s)	1545
ZrmI	AGTACT	2 cut(s)	128, 1269
Zsp2I	ATGCAT	1 cut(s)	395

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.