AT1G67570

Protein of unknown function (DUF3537)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007442
Physical Location & Seq
Forward (+)
25325138 .. 25327286
2149 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G67570.2

Sequence Viewer

Length: 1371 bp
ATGGAGAGTCAGCAACAACAATCACCGGCCGTTGAAACGCCTTTGTTGCTCAATTCTAATCAGGATCATCAAGACCGACCTTCGCCGGAAATCGACGGCGGCGGTGGCGGCGGAGGAGGAGAAGGTGTTAACGACGATCTAGACCGAACGCTAGAATGGTTAGAGACGTTTTTGACGCTCCTTGGGTTTAATCAATCGTCTAAACAAAGCCTTGTTCTGTCTTGGATCGTGTTTTTATCGATTGGTTTGGTGCTTCCGGTGACAGTGTTGGAGCTAGGTCATTGTTTAGGGTGTGAGAGATATCAGTACAAGAGTTTCGAGCTCAACATCGTTGTTTCGCAGGCGTTGTTAGCTGGTGTTTCTCTGCTTTGCGTTTCTCATAATTTGAGAAAACATGGGATTCGAAAGTTTTTGTTTGTTGATCAACTTAGTGGTCGAATGGATCGTCTCAAAGCTCAATACATTCAACAAATCTTGAACTCTGTAAGATTGCTTGCAGTTTGGTCACTTCCATGTTTTGCTTTGAAGGGTGTTCGTGAGATCATCAGAATGTATTATGTGCCTCATGACCAGCCATGGTTATCTGTTGCAATTCTCTTGTCTATGATCTTGTCGTGGACTTACTTAAGCACTATCTTTCTAGCTGCAAGTGCTATGTTTCATTTAGTATGTAACTTGCAAGTTATTCACTTTGAAGATTATGCAAAGCTTTTAGAAGGGGAGTCTGAAATCTCTCTTTTCATCTATGAGCATATGCGTTTGCGTCATTATCTTTCGAAAATAAGCCATAGGTTCCGCATCTTTCTGCTTCTACAGTTTCTGGTTGTCACAGCTAGCCAGTTTACTACGCTTTTTCAGACCACTGCGTATAGTGGGCGTATCACTTATATAAACGGTGGTGATTTTGCAGTCTCTGCGGTTGTCCAAGTTGTTGGAATCATTTTGTGTCTACACGCAGCAACAAAAATATCACACAGAGCTCAAGCAATAGCATCAGTTGCAAGTAGATGGCATGCGATGATGTCTTGCTCGTCTACAGATTCAACTCAGATAAGGGCATCTCCTAGTGGAGTTCATTTGGAGGCTACCACGAATCCGCCCATCTCCTTTCCGATTTCCCGTTCAGATAGTGATGTGGAATCAATGGATCACTACATGAGGATGCCTGTTACTAACCAGTTTCCTTCTTATATGTCCATGTCCTCATATCACAAACGACAAGCTTTCGTGTTGTATTTGCAGATGAATCCAGGCGGGATAACGATATTCGGGTGGACAGTAGACAGGCATTTGATAAACACAATATTCTTCATTGAGCTCTCTCTAGTTACTTTTGTACTCGGCAAGACTGTAGTTTTCGGTACTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3537

Protein Analysis

456

Amino Acids

51.26

Weight (kDa)

6.78

Isoelectric Point (pI)

49.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3537 PF12056 50 - 409 3.4e-128 Protein of unknown function (DUF3537)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 949, 1034, 1281
AciI CCGC 8 cut(s) 99, 102, 108, 111, 796, 917, 1097, 1254
AclWI GGATC 4 cut(s) 72, 233, 450, 1155
AcoI YGGCCR 1 cut(s) 27
AfaI GTAC 3 cut(s) 308, 1338, 1363
AflII CTTAAG 1 cut(s) 625
AgsI TTSAA 6 cut(s) 35, 467, 478, 526, 695, 1044
AjnI CCWGG 1 cut(s) 1249
Alw21I GWGCWC 3 cut(s) 324, 982, 1320
Alw26I GTCTC 3 cut(s) 158, 452, 916
AlwI GGATC 4 cut(s) 72, 233, 450, 1155
AlwNI CAGNNNCTG 2 cut(s) 820, 914
AoxI GGCC 1 cut(s) 27
ApeKI GCWGC 2 cut(s) 644, 956
ArsI GACNNNNNNTTYG 2 cut(s) 430, 462
AsuHPI GGTGA 3 cut(s) 15, 271, 911
AsuII TTCGAA 2 cut(s) 403, 776
AsuNHI GCTAGC 1 cut(s) 833
BanII GRGCYC 3 cut(s) 324, 982, 1320
Bbv12I GWGCWC 3 cut(s) 324, 982, 1320
BbvI GCAGC 2 cut(s) 631, 968
BccI CCATC 2 cut(s) 1002, 1109
BceAI ACGGC 2 cut(s) 14, 112
BciT130I CCWGG 1 cut(s) 1251
BclI TGATCA 1 cut(s) 421
BcoDI GTCTC 3 cut(s) 158, 452, 916
BfaI CTAG 7 cut(s) 140, 152, 275, 641, 834, 1065, 1325
BfmI CTRYAG 3 cut(s) 812, 1035, 1350
BfrI CTTAAG 1 cut(s) 625
BisI GCNGC 4 cut(s) 100, 109, 645, 957
BlsI GCNGC 4 cut(s) 101, 110, 646, 958
Bme1390I CCNGG 1 cut(s) 1251
BmiI GGNNCC 1 cut(s) 794
BmrFI CCNGG 1 cut(s) 1251
BmsI GCATC 4 cut(s) 807, 1001, 1067, 1152
BmtI GCTAGC 1 cut(s) 837
Bpu14I TTCGAA 2 cut(s) 403, 776
BpuEI CTTGAG 1 cut(s) 966
Bsa29I ATCGAT 1 cut(s) 239
BsaJI CCNNGG 2 cut(s) 181, 575
BsaWI WCCGGW 1 cut(s) 256
BsaXI ACNNNNNCTCC 2 cut(s) 111, 141
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 2 cut(s) 838, 1177
BseBI CCWGG 1 cut(s) 1251
BseCI ATCGAT 1 cut(s) 239
BseDI CCNNGG 2 cut(s) 181, 575
BseGI GGATG 1 cut(s) 1167
BseMII CTCAG 1 cut(s) 1061
BseNI ACTGG 2 cut(s) 838, 1177
BseRI GAGGAG 2 cut(s) 129, 132
BseX3I CGGCCG 1 cut(s) 27
BseXI GCAGC 2 cut(s) 631, 968
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 1 cut(s) 29
BshVI ATCGAT 1 cut(s) 239
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 3 cut(s) 324, 982, 1320
BsiSI CCGG 3 cut(s) 26, 86, 257
BsmAI GTCTC 3 cut(s) 158, 452, 916
BsmBI CGTCTC 2 cut(s) 158, 452
BsnI GGCC 1 cut(s) 29
Bsp119I TTCGAA 2 cut(s) 403, 776
Bsp1286I GDGCHC 3 cut(s) 324, 982, 1320
Bsp143I GATC 8 cut(s) 64, 136, 225, 421, 442, 540, 606, 1147
Bsp19I CCATGG 1 cut(s) 575
BspACI CCGC 8 cut(s) 99, 102, 108, 111, 796, 917, 1097, 1254
BspANI GGCC 1 cut(s) 29
BspCNI CTCAG 1 cut(s) 1060
BspDI ATCGAT 1 cut(s) 239
BspHI TCATGA 1 cut(s) 565
BspLI GGNNCC 1 cut(s) 794
BspOI GCTAGC 1 cut(s) 837
BspPI GGATC 4 cut(s) 72, 233, 450, 1155
BspT104I TTCGAA 2 cut(s) 403, 776
BspTI CTTAAG 1 cut(s) 625
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 2 cut(s) 838, 1177
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 2 cut(s) 181, 575
BssMI GATC 8 cut(s) 64, 136, 225, 421, 442, 540, 606, 1147
BssT1I CCWWGG 2 cut(s) 181, 575
Bst2UI CCWGG 1 cut(s) 1251
Bst4CI ACNGT 5 cut(s) 265, 816, 896, 1279, 1351
BstAFI CTTAAG 1 cut(s) 625
BstAPI GCANNNNNTGC 2 cut(s) 914, 998
BstBI TTCGAA 2 cut(s) 403, 776
BstC8I GCNNGC 4 cut(s) 342, 495, 835, 1014
BstDEI CTNAG 2 cut(s) 428, 1047
BstDSI CCRYGG 1 cut(s) 575
BstF5I GGATG 1 cut(s) 1167
BstKTI GATC 8 cut(s) 67, 139, 228, 424, 445, 543, 609, 1150
BstMAI GTCTC 3 cut(s) 158, 452, 916
BstMBI GATC 8 cut(s) 64, 136, 225, 421, 442, 540, 606, 1147
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 7 cut(s) 46, 105, 108, 350, 650, 914, 998
BstNI CCWGG 1 cut(s) 1251
BstNSI RCATGY 1 cut(s) 1016
BstSCI CCNGG 1 cut(s) 1249
BstSFI CTRYAG 3 cut(s) 812, 1035, 1350
BstV1I GCAGC 2 cut(s) 631, 968
BstXI CCANNNNNNTGG 1 cut(s) 932
BstZI CGGCCG 1 cut(s) 27
Bsu15I ATCGAT 1 cut(s) 239
BsuRI GGCC 1 cut(s) 29
BsuTUI ATCGAT 1 cut(s) 239
BtgI CCRYGG 1 cut(s) 575
BtgZI GCGATG 1 cut(s) 1031
BtsCI GGATG 1 cut(s) 1167
BtsI GCAGTG 1 cut(s) 861
BtsIMutI CAGTG 2 cut(s) 270, 861
Cac8I GCNNGC 4 cut(s) 342, 495, 835, 1014
CaiI CAGNNNCTG 2 cut(s) 820, 914
CciI TCATGA 1 cut(s) 565
Cfr10I RCCGGY 1 cut(s) 25
ClaI ATCGAT 1 cut(s) 239
CseI GACGC 2 cut(s) 184, 752
Csp6I GTAC 3 cut(s) 307, 1337, 1362
CviAII CATG 7 cut(s) 395, 513, 566, 576, 1013, 1156, 1198
CviQI GTAC 3 cut(s) 307, 1337, 1362
DdeI CTNAG 2 cut(s) 428, 1047
DpnI GATC 8 cut(s) 66, 138, 227, 423, 444, 542, 608, 1149
DpnII GATC 8 cut(s) 64, 136, 225, 421, 442, 540, 606, 1147
EaeI YGGCCR 1 cut(s) 27
EagI CGGCCG 1 cut(s) 27
EciI GGCGGA 2 cut(s) 126, 1086
Ecl136II GAGCTC 3 cut(s) 322, 980, 1318
EclXI CGGCCG 1 cut(s) 27
Eco130I CCWWGG 2 cut(s) 181, 575
Eco24I GRGCYC 3 cut(s) 324, 982, 1320
Eco32I GATATC 1 cut(s) 302
Eco52I CGGCCG 1 cut(s) 27
Eco53kI GAGCTC 3 cut(s) 322, 980, 1318
EcoICRI GAGCTC 3 cut(s) 322, 980, 1318
EcoRII CCWGG 1 cut(s) 1249
EcoRV GATATC 1 cut(s) 302
EcoT14I CCWWGG 2 cut(s) 181, 575
EcoT38I GRGCYC 3 cut(s) 324, 982, 1320
ErhI CCWWGG 2 cut(s) 181, 575
Esp3I CGTCTC 2 cut(s) 158, 452
FaeI CATG 7 cut(s) 398, 516, 569, 579, 1016, 1159, 1201
FatI CATG 7 cut(s) 394, 512, 565, 575, 1012, 1155, 1197
FauI CCCGC 1 cut(s) 1247
FauNDI CATATG 1 cut(s) 753
FbaI TGATCA 1 cut(s) 421
FblI GTMKAC 3 cut(s) 949, 1034, 1281
Fnu4HI GCNGC 4 cut(s) 100, 109, 645, 957
FokI GGATG 1 cut(s) 1174
FriOI GRGCYC 3 cut(s) 324, 982, 1320
Fsp4HI GCNGC 4 cut(s) 100, 109, 645, 957
FspBI CTAG 7 cut(s) 140, 152, 275, 641, 834, 1065, 1325
GluI GCNGC 4 cut(s) 100, 109, 645, 957
HaeIII GGCC 1 cut(s) 29
HapII CCGG 3 cut(s) 26, 86, 257
HgaI GACGC 2 cut(s) 184, 752
Hin1II CATG 7 cut(s) 398, 516, 569, 579, 1016, 1159, 1201
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HindIII AAGCTT 2 cut(s) 707, 1221
HinfI GANTC 8 cut(s) 7, 400, 722, 936, 1040, 1093, 1139, 1246
HpaI GTTAAC 1 cut(s) 130
HpaII CCGG 3 cut(s) 26, 86, 257
HphI GGTGA 3 cut(s) 15, 271, 911
Hpy166II GTNNAC 7 cut(s) 130, 618, 843, 950, 1035, 1275, 1282
Hpy188I TCNGA 6 cut(s) 548, 727, 858, 1050, 1113, 1126
Hpy188III TCNNGA 6 cut(s) 62, 71, 140, 475, 536, 566
Hpy8I GTNNAC 7 cut(s) 130, 618, 843, 950, 1035, 1275, 1282
Hpy99I CGWCG 2 cut(s) 98, 137
HpyAV CCTTC 5 cut(s) 90, 116, 520, 710, 1194
HpyCH4III ACNGT 5 cut(s) 265, 816, 896, 1279, 1351
HpyCH4IV ACGT 1 cut(s) 167
HpyCH4V TGCA 8 cut(s) 497, 590, 647, 679, 704, 908, 1001, 1240
HpyF10VI GCNNNNNNNGC 7 cut(s) 46, 105, 108, 350, 650, 914, 998
HpyF3I CTNAG 2 cut(s) 428, 1047
HpySE526I ACGT 1 cut(s) 167
Hsp92II CATG 7 cut(s) 398, 516, 569, 579, 1016, 1159, 1201
Ksp22I TGATCA 1 cut(s) 421
KspAI GTTAAC 1 cut(s) 130
Kzo9I GATC 8 cut(s) 64, 136, 225, 421, 442, 540, 606, 1147
LmnI GCTCC 2 cut(s) 183, 271
Lsp1109I GCAGC 2 cut(s) 631, 968
LweI GCATC 4 cut(s) 807, 1001, 1067, 1152
MaeI CTAG 7 cut(s) 140, 152, 275, 641, 834, 1065, 1325
MaeII ACGT 1 cut(s) 167
MaeIII GTNAC 6 cut(s) 259, 504, 671, 826, 1168, 1327
MalI GATC 8 cut(s) 66, 138, 227, 423, 444, 542, 608, 1149
MboI GATC 8 cut(s) 64, 136, 225, 421, 442, 540, 606, 1147
MboII GAAGA 2 cut(s) 707, 1300
MhlI GDGCHC 3 cut(s) 324, 982, 1320
MluCI AATT 3 cut(s) 52, 382, 591
MlyI GAGTC 2 cut(s) 16, 731
MmeI TCCRAC 2 cut(s) 249, 913
MnlI CCTC 6 cut(s) 107, 110, 573, 1075, 1152, 1213
MseI TTAA 3 cut(s) 129, 189, 626
MslI CAYNNNNRTG 3 cut(s) 511, 548, 1160
MspCI CTTAAG 1 cut(s) 625
MspI CCGG 3 cut(s) 26, 86, 257
MspR9I CCNGG 1 cut(s) 1251
MvaI CCWGG 1 cut(s) 1251
MwoI GCNNNNNNNGC 7 cut(s) 46, 105, 108, 350, 650, 914, 998
NcoI CCATGG 1 cut(s) 575
NdeI CATATG 1 cut(s) 753
NdeII GATC 8 cut(s) 64, 136, 225, 421, 442, 540, 606, 1147
NheI GCTAGC 1 cut(s) 833
NlaIII CATG 7 cut(s) 398, 516, 569, 579, 1016, 1159, 1201
NlaIV GGNNCC 1 cut(s) 794
NmeAIII GCCGAG 1 cut(s) 1320
NmuCI GTSAC 3 cut(s) 259, 504, 826
NspI RCATGY 1 cut(s) 1016
NspV TTCGAA 2 cut(s) 403, 776
PaeI GCATGC 1 cut(s) 1016
PagI TCATGA 1 cut(s) 565
PcsI WCGNNNNNNNCGW 1 cut(s) 442
PfeI GAWTC 6 cut(s) 400, 936, 1040, 1093, 1139, 1246
PkrI GCNGC 4 cut(s) 101, 110, 646, 958
PleI GAGTC 2 cut(s) 15, 730
PpsI GAGTC 2 cut(s) 15, 730
Psp124BI GAGCTC 3 cut(s) 324, 982, 1320
Psp6I CCWGG 1 cut(s) 1249
PspGI CCWGG 1 cut(s) 1249
PspN4I GGNNCC 1 cut(s) 794
PstNI CAGNNNCTG 2 cut(s) 820, 914
RsaI GTAC 3 cut(s) 308, 1338, 1363
RsaNI GTAC 3 cut(s) 307, 1337, 1362
RseI CAYNNNNRTG 3 cut(s) 511, 548, 1160
SacI GAGCTC 3 cut(s) 324, 982, 1320
SaqAI TTAA 3 cut(s) 129, 189, 626
SatI GCNGC 4 cut(s) 100, 109, 645, 957
Sau3AI GATC 8 cut(s) 64, 136, 225, 421, 442, 540, 606, 1147
SchI GAGTC 2 cut(s) 16, 731
ScrFI CCNGG 1 cut(s) 1251
SduI GDGCHC 3 cut(s) 324, 982, 1320
SfaNI GCATC 4 cut(s) 807, 1001, 1067, 1152
SfcI CTRYAG 3 cut(s) 812, 1035, 1350
SfuI TTCGAA 2 cut(s) 403, 776
SmiMI CAYNNNNRTG 3 cut(s) 511, 548, 1160
SmlI CTYRAG 2 cut(s) 625, 981
SmoI CTYRAG 2 cut(s) 625, 981
SphI GCATGC 1 cut(s) 1016
Sse9I AATT 3 cut(s) 52, 382, 591
SsiI CCGC 8 cut(s) 99, 102, 108, 111, 796, 917, 1097, 1254
SspI AATATT 1 cut(s) 1305
SspMI CTAG 7 cut(s) 140, 152, 275, 641, 834, 1065, 1325
SstI GAGCTC 3 cut(s) 324, 982, 1320
StyD4I CCNGG 1 cut(s) 1249
StyI CCWWGG 2 cut(s) 181, 575
TaaI ACNGT 5 cut(s) 265, 816, 896, 1279, 1351
TaiI ACGT 1 cut(s) 170
TaqI TCGA 6 cut(s) 93, 239, 318, 403, 436, 776
TaqII GACCGA 2 cut(s) 90, 159
TasI AATT 3 cut(s) 52, 382, 591
TatI WGTACW 2 cut(s) 306, 1336
TauI GCSGC 2 cut(s) 102, 111
TfiI GAWTC 6 cut(s) 400, 936, 1040, 1093, 1139, 1246
Tru1I TTAA 3 cut(s) 129, 189, 626
Tru9I TTAA 3 cut(s) 129, 189, 626
TscAI CASTG 2 cut(s) 270, 868
TseFI GTSAC 3 cut(s) 259, 504, 826
TseI GCWGC 2 cut(s) 644, 956
Tsp45I GTSAC 3 cut(s) 259, 504, 826
TspDTI ATGAA 5 cut(s) 650, 730, 1064, 1259, 1300
TspRI CASTG 2 cut(s) 270, 868
Vha464I CTTAAG 1 cut(s) 625
XbaI TCTAGA 1 cut(s) 139
XceI RCATGY 1 cut(s) 1016
XmiI GTMKAC 3 cut(s) 949, 1034, 1281
XspI CTAG 7 cut(s) 140, 152, 275, 641, 834, 1065, 1325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.