MD16G1112800.v1.1

Protein of unknown function (DUF3537)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007442
Physical Location & Seq
Reverse (-)
7891637 .. 7894026
2390 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1112800.v1.1.491

Sequence Viewer

Length: 1377 bp
ATGGCGGACGTTTTTATAAAAACAGAGGAGCCACCTTCCCAACTTATCCCTCTTCTCGATTCTAATAACAATAACAATAACAACGACCACCACATCGATCCCCACAGCAACCGTCATCTGGGAGGAGAAGAAGAATTCAAAGATACCCCATTGGACCAAACCCTCCAATGGCTAGAAACGTTTCTCACTCTCTTGGGCTTCAACCAATCTTCTTGGTTGAGCCTTGCCCTGTCCTGGACGGCTCTGGCTCTCATCGGTGTGGCTCTTCCGGTGGTTGTGCTTGTGGTGTTCAACTGCTCCGACTGTAAGAAGTATCAGATTAAAAGGTTCGAGCTCGTTATCGTGGGCTCGCAAGCGATTCTGGCAGCGGTCTCTGTGCTCTGCATCTCCCCCAGGCTTCGCAAATATGGCATTCGGAGGTTCCTCTTCGTCGATCGCTATAGCGGTCATATCGTTCGGTTTCGAAACGATTACATCAAGCAGATTAAGGATTCTTTCCGATTGCTTGTTCGGTGGGCACTGCCATGTTTTCTTTTGAAGACACTGCGTGAGGTCGTCCGCATGATATACGTCCATCACGATTCATGGGGGTGGGGGAAATCAGTTGGTGTACTGTTCACTTTGGTTCTATCATGGGCTTATGTGAGTACAATCACTCTAACAGCCAGCATTTTGTTTCACTTGGTCTGCAATTTGCAAGTTATCCACTTCGACGATTATGCAAGGCTCCTGGAAAGGGAATCTGATGTGCTGGTATTTATGGACGAGCATATTCGTCTGCGCTACCATCTCTGTAAAATAAGTCACAGGTTCCGAATCTTCCTTCTCCTGCAGTTCATGGTTGTCACCGCAAGCCAGGTTGTCACTCTATTTGAGACCACAGAATACAGGGGAGACATCACTGTCATAAATGGTGGCGATTTTGCAGTCTCCACAATTGTTCAAGTTGTTGGCATTATTCTCTCCCTGCACGCAGCGACCAGAATTTCCCATAGAGCGCAAGGCATAGCGACAGTTGCCAGTAGATGGCATGCGTTAATGACATGCAGTTCTAACGATACATCTCACAACAGAAGTTCAAACTGCTTGGTGAACCCAGAGGCTGCCGACACATTGAACTCGCTGCAAATAAGCTACTCCGAGAGTGACTTGGAGTCGATGGATTACGTTCCAATGCCTACAAATACACAGTTAGCTTCATACATGTCTTCCTATCACAGGAGACAAGCTTTTGTGTCATACTTGCAAACCAATCATGGGGGGATCACAATTTTTGGATGGACAGTCGACAGAGCCCTCCTCAACACAATTTTCTTCATTGAACTGTCTTTGATCACCTTTGTTCTTGGAAAGACATTAGTTTTCACGTCGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3537

Protein Analysis

459

Amino Acids

52.17

Weight (kDa)

7.29

Isoelectric Point (pI)

38.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3537 PF12056 54 - 440 1.8e-145 Protein of unknown function (DUF3537)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 17
AasI GACNNNNNNGTC 1 cut(s) 902
AccB7I CCANNNNNTGG 1 cut(s) 1026
AccI GTMKAC 2 cut(s) 1287, 1370
AciI CCGC 5 cut(s) 5, 368, 444, 559, 849
AclI AACGTT 1 cut(s) 179
AclWI GGATC 2 cut(s) 92, 1271
AcsI RAATTY 2 cut(s) 134, 984
AdeI CACNNNGTG 1 cut(s) 548
AfaI GTAC 2 cut(s) 612, 649
AfiI CCNNNNNNNGG 7 cut(s) 118, 168, 234, 736, 1026, 1218, 1257
AflIII ACRYGT 1 cut(s) 1203
AgsI TTSAA 8 cut(s) 139, 202, 292, 538, 944, 1080, 1117, 1322
AhdI GACNNNNNGTC 1 cut(s) 1153
AjiI CACGTC 2 cut(s) 1368, 1374
AjnI CCWGG 4 cut(s) 233, 392, 729, 855
AluBI AGCT 4 cut(s) 334, 1134, 1196, 1229
AluI AGCT 4 cut(s) 334, 1134, 1196, 1229
Alw21I GWGCWC 2 cut(s) 336, 381
Alw26I GTCTC 5 cut(s) 376, 869, 888, 934, 1216
AlwI GGATC 2 cut(s) 92, 1271
AlwNI CAGNNNCTG 1 cut(s) 1103
ApeKI GCWGC 4 cut(s) 365, 974, 1103, 1123
ApoI RAATTY 2 cut(s) 134, 984
Asp700I GAANNNNTTC 1 cut(s) 180
AspLEI GCGC 2 cut(s) 783, 1000
AspS9I GGNCC 1 cut(s) 154
AsuHPI GGTGA 3 cut(s) 838, 1102, 1327
AsuII TTCGAA 1 cut(s) 463
AvaII GGWCC 1 cut(s) 154
BaeGI GKGCMC 1 cut(s) 520
BanII GRGCYC 3 cut(s) 336, 350, 1297
BbsI GAAGAC 2 cut(s) 545, 1200
Bbv12I GWGCWC 2 cut(s) 336, 381
BbvI GCAGC 4 cut(s) 377, 986, 1090, 1110
BccI CCATC 5 cut(s) 582, 795, 1020, 1153, 1272
BceAI ACGGC 1 cut(s) 255
BcgI CGANNNNNNTGC 2 cut(s) 701, 735
BciT130I CCWGG 4 cut(s) 235, 394, 731, 857
BclI TGATCA 1 cut(s) 1332
BcoDI GTCTC 5 cut(s) 376, 869, 888, 934, 1216
BfaI CTAG 1 cut(s) 173
BfmI CTRYAG 2 cut(s) 439, 830
BisI GCNGC 4 cut(s) 366, 975, 1104, 1124
BlsI GCNGC 4 cut(s) 367, 976, 1105, 1125
Bme1390I CCNGG 4 cut(s) 235, 394, 731, 857
Bme18I GGWCC 1 cut(s) 154
BmeRI GACNNNNNGTC 1 cut(s) 1153
BmgBI CACGTC 2 cut(s) 1368, 1374
BmgT120I GGNCC 1 cut(s) 154
BmiI GGNNCC 4 cut(s) 30, 422, 728, 812
BmrFI CCNGG 4 cut(s) 235, 394, 731, 857
BmsI GCATC 1 cut(s) 393
BpiI GAAGAC 2 cut(s) 545, 1200
BplI GAGNNNNNCTC 2 cut(s) 1284, 1316
Bpu14I TTCGAA 1 cut(s) 463
Bsa29I ATCGAT 1 cut(s) 96
BsaI GGTCTC 2 cut(s) 376, 869
BsaJI CCNNGG 1 cut(s) 392
BsaWI WCCGGW 1 cut(s) 268
Bsc4I CCNNNNNNNGG 7 cut(s) 118, 168, 234, 736, 1026, 1218, 1257
Bse1I ACTGG 1 cut(s) 1020
BseBI CCWGG 4 cut(s) 235, 394, 731, 857
BseCI ATCGAT 1 cut(s) 96
BseDI CCNNGG 1 cut(s) 392
BseGI GGATG 1 cut(s) 1283
BseLI CCNNNNNNNGG 7 cut(s) 118, 168, 234, 736, 1026, 1218, 1257
BseNI ACTGG 1 cut(s) 1020
BseRI GAGGAG 3 cut(s) 41, 138, 1289
BseSI GKGCMC 1 cut(s) 520
BseXI GCAGC 4 cut(s) 377, 986, 1090, 1110
BsgI GTGCAG 1 cut(s) 953
Bsh1285I CGRYCG 1 cut(s) 436
BshVI ATCGAT 1 cut(s) 96
BsiEI CGRYCG 1 cut(s) 436
BsiHKAI GWGCWC 2 cut(s) 336, 381
BsiSI CCGG 1 cut(s) 269
BslI CCNNNNNNNGG 7 cut(s) 118, 168, 234, 736, 1026, 1218, 1257
BsmAI GTCTC 5 cut(s) 376, 869, 888, 934, 1216
BsmI GAATGC 1 cut(s) 411
Bso31I GGTCTC 2 cut(s) 376, 869
Bsp119I TTCGAA 1 cut(s) 463
Bsp1286I GDGCHC 5 cut(s) 336, 350, 381, 520, 1297
Bsp143I GATC 4 cut(s) 97, 433, 1263, 1332
BspACI CCGC 5 cut(s) 5, 368, 444, 559, 849
BspDI ATCGAT 1 cut(s) 96
BspLI GGNNCC 4 cut(s) 30, 422, 728, 812
BspMAI CTGCAG 1 cut(s) 834
BspPI GGATC 2 cut(s) 92, 1271
BspQI GCTCTTC 1 cut(s) 270
BspT104I TTCGAA 1 cut(s) 463
BspTNI GGTCTC 2 cut(s) 376, 869
BsrI ACTGG 1 cut(s) 1020
BssECI CCNNGG 1 cut(s) 392
BssMI GATC 4 cut(s) 97, 433, 1263, 1332
Bst2UI CCWGG 4 cut(s) 235, 394, 731, 857
Bst4CI ACNGT 8 cut(s) 113, 305, 615, 904, 1015, 1191, 1285, 1326
Bst6I CTCTTC 3 cut(s) 57, 270, 431
BstBI TTCGAA 1 cut(s) 463
BstC8I GCNNGC 6 cut(s) 350, 354, 667, 853, 972, 1032
BstENI CCTNNNNNAGG 1 cut(s) 1216
BstF5I GGATG 1 cut(s) 1283
BstHHI GCGC 2 cut(s) 783, 1000
BstKTI GATC 4 cut(s) 100, 436, 1266, 1335
BstMAI GTCTC 5 cut(s) 376, 869, 888, 934, 1216
BstMBI GATC 4 cut(s) 97, 433, 1263, 1332
BstMCI CGRYCG 1 cut(s) 436
BstMWI GCNNNNNNNGC 3 cut(s) 362, 408, 1016
BstNI CCWGG 4 cut(s) 235, 394, 731, 857
BstNSI RCATGY 3 cut(s) 1034, 1047, 1207
BstSCI CCNGG 4 cut(s) 233, 392, 729, 855
BstSFI CTRYAG 2 cut(s) 439, 830
BstSLI GKGCMC 1 cut(s) 520
BstV1I GCAGC 4 cut(s) 377, 986, 1090, 1110
BstV2I GAAGAC 2 cut(s) 545, 1200
Bsu15I ATCGAT 1 cut(s) 96
BsuTUI ATCGAT 1 cut(s) 96
BtrI CACGTC 2 cut(s) 1368, 1374
BtsCI GGATG 1 cut(s) 1283
BtsI GCAGTG 2 cut(s) 518, 542
BtsIMutI CAGTG 3 cut(s) 518, 542, 900
Cac8I GCNNGC 6 cut(s) 350, 354, 667, 853, 972, 1032
CaiI CAGNNNCTG 1 cut(s) 1103
CfoI GCGC 2 cut(s) 783, 1000
Cfr13I GGNCC 1 cut(s) 154
ClaI ATCGAT 1 cut(s) 96
Csp6I GTAC 2 cut(s) 611, 648
CviAII CATG 9 cut(s) 525, 562, 585, 633, 838, 1031, 1044, 1204, 1256
CviQI GTAC 2 cut(s) 611, 648
DpnI GATC 4 cut(s) 99, 435, 1265, 1334
DpnII GATC 4 cut(s) 97, 433, 1263, 1332
DraIII CACNNNGTG 1 cut(s) 548
DrdI GACNNNNNNGTC 1 cut(s) 902
DriI GACNNNNNGTC 1 cut(s) 1153
DseDI GACNNNNNNGTC 1 cut(s) 902
Eam1104I CTCTTC 3 cut(s) 57, 270, 431
Eam1105I GACNNNNNGTC 1 cut(s) 1153
EarI CTCTTC 3 cut(s) 57, 270, 431
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 334
Eco24I GRGCYC 3 cut(s) 336, 350, 1297
Eco31I GGTCTC 2 cut(s) 376, 869
Eco47I GGWCC 1 cut(s) 154
Eco53kI GAGCTC 1 cut(s) 334
EcoICRI GAGCTC 1 cut(s) 334
EcoNI CCTNNNNNAGG 1 cut(s) 1216
EcoRI GAATTC 1 cut(s) 134
EcoRII CCWGG 4 cut(s) 233, 392, 729, 855
EcoT38I GRGCYC 3 cut(s) 336, 350, 1297
FaeI CATG 9 cut(s) 528, 565, 588, 636, 841, 1034, 1047, 1207, 1259
FatI CATG 9 cut(s) 524, 561, 584, 632, 837, 1030, 1043, 1203, 1255
FbaI TGATCA 1 cut(s) 1332
FblI GTMKAC 2 cut(s) 1287, 1370
Fnu4HI GCNGC 4 cut(s) 366, 975, 1104, 1124
FokI GGATG 1 cut(s) 1290
FriOI GRGCYC 3 cut(s) 336, 350, 1297
Fsp4HI GCNGC 4 cut(s) 366, 975, 1104, 1124
FspBI CTAG 1 cut(s) 173
GlaI GCGC 2 cut(s) 782, 999
GluI GCNGC 4 cut(s) 366, 975, 1104, 1124
HapII CCGG 1 cut(s) 269
HhaI GCGC 2 cut(s) 783, 1000
Hin1II CATG 9 cut(s) 528, 565, 588, 636, 841, 1034, 1047, 1207, 1259
Hin6I GCGC 2 cut(s) 781, 998
HinP1I GCGC 2 cut(s) 781, 998
HincII GTYRAC 2 cut(s) 1288, 1371
HindII GTYRAC 2 cut(s) 1288, 1371
HindIII AAGCTT 1 cut(s) 1227
HinfI GANTC 7 cut(s) 59, 358, 491, 581, 740, 816, 1154
HpaII CCGG 1 cut(s) 269
HphI GGTGA 3 cut(s) 838, 1102, 1327
Hpy166II GTNNAC 5 cut(s) 611, 618, 1093, 1288, 1371
Hpy188I TCNGA 7 cut(s) 301, 318, 417, 500, 745, 815, 1141
Hpy188III TCNNGA 2 cut(s) 56, 578
Hpy8I GTNNAC 5 cut(s) 611, 618, 1093, 1288, 1371
Hpy99I CGWCG 4 cut(s) 434, 716, 1372, 1375
HpyAV CCTTC 2 cut(s) 45, 833
HpyCH4III ACNGT 8 cut(s) 113, 305, 615, 904, 1015, 1191, 1285, 1326
HpyCH4IV ACGT 6 cut(s) 9, 179, 570, 1167, 1367, 1373
HpyF10VI GCNNNNNNNGC 3 cut(s) 362, 408, 1016
HpySE526I ACGT 6 cut(s) 9, 179, 570, 1167, 1367, 1373
Hsp92II CATG 9 cut(s) 528, 565, 588, 636, 841, 1034, 1047, 1207, 1259
HspAI GCGC 2 cut(s) 781, 998
Ksp22I TGATCA 1 cut(s) 1332
Kzo9I GATC 4 cut(s) 97, 433, 1263, 1332
LguI GCTCTTC 1 cut(s) 270
LmnI GCTCC 3 cut(s) 28, 302, 732
Lsp1109I GCAGC 4 cut(s) 377, 986, 1090, 1110
LweI GCATC 1 cut(s) 393
MaeI CTAG 1 cut(s) 173
MaeII ACGT 6 cut(s) 9, 179, 570, 1167, 1367, 1373
MaeIII GTNAC 4 cut(s) 803, 844, 862, 1145
MalI GATC 4 cut(s) 99, 435, 1265, 1334
MboI GATC 4 cut(s) 97, 433, 1263, 1332
MfeI CAATTG 1 cut(s) 936
MhlI GDGCHC 5 cut(s) 336, 350, 381, 520, 1297
MluCI AATT 6 cut(s) 134, 691, 936, 984, 1269, 1308
MlyI GAGTC 1 cut(s) 1163
MmeI TCCRAC 1 cut(s) 324
MroXI GAANNNNTTC 1 cut(s) 180
MseI TTAA 3 cut(s) 321, 486, 1037
MslI CAYNNNNRTG 3 cut(s) 257, 523, 589
MspA1I CMGCKG 1 cut(s) 368
MspI CCGG 1 cut(s) 269
MspR9I CCNGG 4 cut(s) 235, 394, 731, 857
MunI CAATTG 1 cut(s) 936
Mva1269I GAATGC 1 cut(s) 411
MvaI CCWGG 4 cut(s) 235, 394, 731, 857
MwoI GCNNNNNNNGC 3 cut(s) 362, 408, 1016
NdeII GATC 4 cut(s) 97, 433, 1263, 1332
NlaIII CATG 9 cut(s) 528, 565, 588, 636, 841, 1034, 1047, 1207, 1259
NlaIV GGNNCC 4 cut(s) 30, 422, 728, 812
NmuCI GTSAC 4 cut(s) 803, 844, 862, 1145
NspI RCATGY 3 cut(s) 1034, 1047, 1207
NspV TTCGAA 1 cut(s) 463
PaeI GCATGC 1 cut(s) 1034
PciI ACATGT 1 cut(s) 1203
PciSI GCTCTTC 1 cut(s) 270
PcsI WCGNNNNNNNCGW 1 cut(s) 576
PctI GAATGC 1 cut(s) 411
PdmI GAANNNNTTC 1 cut(s) 180
PfeI GAWTC 6 cut(s) 59, 358, 491, 581, 740, 816
PflMI CCANNNNNTGG 1 cut(s) 1026
PfoI TCCNGGA 2 cut(s) 233, 729
PkrI GCNGC 4 cut(s) 367, 976, 1105, 1125
Ple19I CGATCG 1 cut(s) 436
PleI GAGTC 1 cut(s) 1162
PpsI GAGTC 1 cut(s) 1162
PscI ACATGT 1 cut(s) 1203
PsiI TTATAA 1 cut(s) 17
Psp124BI GAGCTC 1 cut(s) 336
Psp1406I AACGTT 1 cut(s) 179
Psp6I CCWGG 4 cut(s) 233, 392, 729, 855
PspGI CCWGG 4 cut(s) 233, 392, 729, 855
PspN4I GGNNCC 4 cut(s) 30, 422, 728, 812
PspPI GGNCC 1 cut(s) 154
PstI CTGCAG 1 cut(s) 834
PstNI CAGNNNCTG 1 cut(s) 1103
PvuI CGATCG 1 cut(s) 436
RsaI GTAC 2 cut(s) 612, 649
RsaNI GTAC 2 cut(s) 611, 648
RseI CAYNNNNRTG 3 cut(s) 257, 523, 589
SacI GAGCTC 1 cut(s) 336
SalI GTCGAC 2 cut(s) 1286, 1369
SapI GCTCTTC 1 cut(s) 270
SaqAI TTAA 3 cut(s) 321, 486, 1037
SatI GCNGC 4 cut(s) 366, 975, 1104, 1124
Sau3AI GATC 4 cut(s) 97, 433, 1263, 1332
Sau96I GGNCC 1 cut(s) 154
SchI GAGTC 1 cut(s) 1163
ScrFI CCNGG 4 cut(s) 235, 394, 731, 857
SduI GDGCHC 5 cut(s) 336, 350, 381, 520, 1297
SfaNI GCATC 1 cut(s) 393
SfcI CTRYAG 2 cut(s) 439, 830
SfuI TTCGAA 1 cut(s) 463
SgrDI CGTCGACG 1 cut(s) 1369
SinI GGWCC 1 cut(s) 154
SmiMI CAYNNNNRTG 3 cut(s) 257, 523, 589
SphI GCATGC 1 cut(s) 1034
Sse9I AATT 6 cut(s) 134, 691, 936, 984, 1269, 1308
SsiI CCGC 5 cut(s) 5, 368, 444, 559, 849
SspMI CTAG 1 cut(s) 173
SstI GAGCTC 1 cut(s) 336
StyD4I CCNGG 4 cut(s) 233, 392, 729, 855
TaaI ACNGT 8 cut(s) 113, 305, 615, 904, 1015, 1191, 1285, 1326
TaiI ACGT 6 cut(s) 12, 182, 573, 1170, 1370, 1376
TaqI TCGA 9 cut(s) 57, 96, 330, 432, 463, 711, 1157, 1287, 1370
TasI AATT 6 cut(s) 134, 691, 936, 984, 1269, 1308
TatI WGTACW 2 cut(s) 610, 647
TfiI GAWTC 6 cut(s) 59, 358, 491, 581, 740, 816
Tru1I TTAA 3 cut(s) 321, 486, 1037
Tru9I TTAA 3 cut(s) 321, 486, 1037
TscAI CASTG 3 cut(s) 525, 549, 907
TseFI GTSAC 4 cut(s) 803, 844, 862, 1145
TseI GCWGC 4 cut(s) 365, 974, 1103, 1123
Tsp45I GTSAC 4 cut(s) 803, 844, 862, 1145
TspDTI ATGAA 4 cut(s) 573, 826, 1188, 1306
TspRI CASTG 3 cut(s) 525, 549, 907
Van91I CCANNNNNTGG 1 cut(s) 1026
VpaK11BI GGWCC 1 cut(s) 154
XagI CCTNNNNNAGG 1 cut(s) 1216
XapI RAATTY 2 cut(s) 134, 984
XceI RCATGY 3 cut(s) 1034, 1047, 1207
XmiI GTMKAC 2 cut(s) 1287, 1370
XmnI GAANNNNTTC 1 cut(s) 180
XspI CTAG 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.