MD13G1113500.v1.1

Protein of unknown function (DUF3537)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007442
Physical Location & Seq
Reverse (-)
8247622 .. 8249446
1825 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1113500.v1.1.491

Sequence Viewer

Length: 1377 bp
ATGGCGGATCTTTTTATAAAAACAGAGGAGCCACCTTCCCAACTTACCCCTCTGCTCCATTCTGATAACAATAACCACCACCACCATAACTACCACCACAACAACCACCACAGCAACCACCACAACAACCACCACCACCTGGGAGGAGAAGAAGAATATAAAGACACGCAATTGGACCAAACCCTCCAATGGCTAGAAACGTTTCTCACTCTCTTGGGTTTCAACCAATCTTCTTGGTTGAGCCTTGCTCTGTCCTGGACGGCCCTGGTTCTCATCGGTGTGGCTCTTCCGGTGGTTGCGCTTGTGGTGTTCAACTGCTCCGACTGTGACAAGTATCAGATTAAAGGGTTCGAGCTAGTTATCGTGGGATCGCAAGCGGTTCTGGCGGCGGTCTCTGTGCTCTGCATCTCTCAGAGTCTTCGCAAGTATGGCATTCGGCGGTTCCTGTCTGTTGATCGCAATAGCGGTCACATGGTTCGGTTTCGAAACGATTATATCAAGCAGATTAAGGATTCTTTCTGCTTGCTCGTTCGGTGGGCGCTCCCATGTTTTATTCTGAAGACTCTACGTGAGGTTGTTCGGATGATATATGTCCATCAGGATTCATGGGGGTGGGGGATGTCGGTCGGCGTACTGTTAGCTTTGGTTCTGTCATGGGCTTATGCGAGTACGATCACTTTAACAGCCAGCATTTTGTTTCACTTGGTCTGCAATTTGCAAGTTATCCACTTCGACGATTTCGGAAGGCTCTTGGAGAGGGAATCTGACATGCTGGTGTTTATGGAGGAGCATATCCGCCTGCGCTACCATCTCTGTAAAATAAGCCACAGGTTCCGGATCTTCCTCCTACTGCAGTTCTTAGTTGTCACAGCTAGCCAGTTTGTGACTCTATTTGAAACCACAGGATACAGTGGAGAAATCAGTGTCGTCTCCACGATTGTTCAAGTTGTCGGCATTATTCTTTCCCTGCACACTGCAACCAGAATTTCCCATAGAGCGCAAGGCATAGCGACAGTTGCCAGTAGATGGAATGCGTTAATGACATGCAGTTCTAACGATACATCTCACAACAGAAGCTCAAACGGCTCAGTGAACTCAGAGGCCGCCAACTCATTAAACTCGCTGCATATAAGTTACTCCGACAGTGATTTGGAGTCGATGGATTATGTTCAAATGCCTACAAACACACAGCTGGCTTCGTACATGTCTTCGTATCACAGGAGACAAGCCTTTGTGTTGTACTTGCAAACCAACATCGGAGGGATCACAATTTTCGGATGGACGGTCGACAGAGGCCTCCTCAACACGATCTTTTTCATCGAACTGTCTTTGATTACCTTTGTGCTTGGAAAGACGTTAGTTTTCTCCTCGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3537

Protein Analysis

459

Amino Acids

52.01

Weight (kDa)

6.81

Isoelectric Point (pI)

41.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3537 PF12056 61 - 440 8.3e-134 Protein of unknown function (DUF3537)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 17
AccB7I CCANNNNNTGG 2 cut(s) 139, 1026
AccI GTMKAC 1 cut(s) 1287
AccIII TCCGGA 1 cut(s) 834
AciI CCGC 8 cut(s) 5, 377, 386, 389, 439, 465, 796, 1104
AclI AACGTT 1 cut(s) 200
AclWI GGATC 4 cut(s) 15, 376, 845, 1271
AcsI RAATTY 1 cut(s) 984
AcuI CTGAAG 1 cut(s) 578
AfaI GTAC 4 cut(s) 633, 670, 1202, 1241
AfiI CCNNNNNNNGG 3 cut(s) 139, 189, 1026
AflIII ACRYGT 1 cut(s) 1203
AgsI TTSAA 5 cut(s) 223, 313, 896, 944, 1172
AjnI CCWGG 3 cut(s) 138, 254, 264
AluBI AGCT 5 cut(s) 355, 641, 872, 1077, 1192
AluI AGCT 5 cut(s) 355, 641, 872, 1077, 1192
Alw21I GWGCWC 1 cut(s) 402
Alw26I GTCTC 3 cut(s) 397, 934, 1216
AlwI GGATC 4 cut(s) 15, 376, 845, 1271
Aor13HI TCCGGA 1 cut(s) 834
AoxI GGCC 3 cut(s) 261, 1101, 1294
ApeKI GCWGC 1 cut(s) 1123
ApoI RAATTY 1 cut(s) 984
Asp700I GAANNNNTTC 1 cut(s) 201
AspLEI GCGC 4 cut(s) 301, 541, 804, 1000
AspS9I GGNCC 2 cut(s) 175, 262
AsuII TTCGAA 1 cut(s) 484
AsuNHI GCTAGC 1 cut(s) 872
AvaII GGWCC 1 cut(s) 175
BbsI GAAGAC 3 cut(s) 410, 566, 1200
Bbv12I GWGCWC 1 cut(s) 402
BbvI GCAGC 1 cut(s) 1110
BccI CCATC 5 cut(s) 603, 816, 1020, 1153, 1272
BceAI ACGGC 2 cut(s) 276, 1099
BciT130I CCWGG 3 cut(s) 140, 256, 266
BciVI GTATCC 1 cut(s) 899
BcoDI GTCTC 3 cut(s) 397, 934, 1216
BfaI CTAG 3 cut(s) 194, 356, 873
BfmI CTRYAG 1 cut(s) 851
BfoI RGCGCY 1 cut(s) 542
BfuI GTATCC 1 cut(s) 899
BisI GCNGC 3 cut(s) 387, 1104, 1124
BlsI GCNGC 3 cut(s) 388, 1105, 1125
Bme1390I CCNGG 3 cut(s) 140, 256, 266
Bme18I GGWCC 1 cut(s) 175
BmgT120I GGNCC 2 cut(s) 175, 262
BmiI GGNNCC 3 cut(s) 30, 443, 833
BmrFI CCNGG 3 cut(s) 140, 256, 266
BmsI GCATC 1 cut(s) 414
BmtI GCTAGC 1 cut(s) 876
BpiI GAAGAC 3 cut(s) 410, 566, 1200
BplI GAGNNNNNCTC 4 cut(s) 232, 264, 1284, 1316
Bpu14I TTCGAA 1 cut(s) 484
BsaAI YACGTR 1 cut(s) 569
BsaI GGTCTC 1 cut(s) 397
BsaJI CCNNGG 2 cut(s) 139, 264
BsaWI WCCGGW 2 cut(s) 289, 834
Bsc4I CCNNNNNNNGG 3 cut(s) 139, 189, 1026
Bse1I ACTGG 2 cut(s) 877, 1020
BseAI TCCGGA 1 cut(s) 834
BseBI CCWGG 3 cut(s) 140, 256, 266
BseDI CCNNGG 2 cut(s) 139, 264
BseGI GGATG 3 cut(s) 588, 624, 1283
BseLI CCNNNNNNNGG 3 cut(s) 139, 189, 1026
BseMII CTCAG 3 cut(s) 425, 1101, 1110
BseNI ACTGG 2 cut(s) 877, 1020
BseRI GAGGAG 5 cut(s) 41, 159, 800, 1289, 1357
BseXI GCAGC 1 cut(s) 1110
BsgI GTGCAG 1 cut(s) 953
Bsh1285I CGRYCG 2 cut(s) 627, 1287
BshFI GGCC 3 cut(s) 263, 1103, 1296
BsiEI CGRYCG 2 cut(s) 627, 1287
BsiHKAI GWGCWC 1 cut(s) 402
BsiSI CCGG 2 cut(s) 290, 835
BslI CCNNNNNNNGG 3 cut(s) 139, 189, 1026
BsmAI GTCTC 3 cut(s) 397, 934, 1216
BsmBI CGTCTC 1 cut(s) 934
BsmI GAATGC 2 cut(s) 432, 1036
BsnI GGCC 3 cut(s) 263, 1103, 1296
Bso31I GGTCTC 1 cut(s) 397
Bsp119I TTCGAA 1 cut(s) 484
Bsp1286I GDGCHC 1 cut(s) 402
Bsp13I TCCGGA 1 cut(s) 834
Bsp143I GATC 7 cut(s) 7, 368, 454, 672, 837, 1263, 1308
BspACI CCGC 8 cut(s) 5, 377, 386, 389, 439, 465, 796, 1104
BspANI GGCC 3 cut(s) 263, 1103, 1296
BspCNI CTCAG 3 cut(s) 424, 1100, 1109
BspEI TCCGGA 1 cut(s) 834
BspLI GGNNCC 3 cut(s) 30, 443, 833
BspMAI CTGCAG 1 cut(s) 855
BspOI GCTAGC 1 cut(s) 876
BspPI GGATC 4 cut(s) 15, 376, 845, 1271
BspQI GCTCTTC 1 cut(s) 291
BspT104I TTCGAA 1 cut(s) 484
BspTNI GGTCTC 1 cut(s) 397
BsrI ACTGG 2 cut(s) 877, 1020
BssECI CCNNGG 2 cut(s) 139, 264
BssMI GATC 7 cut(s) 7, 368, 454, 672, 837, 1263, 1308
Bst2UI CCWGG 3 cut(s) 140, 256, 266
Bst4CI ACNGT 7 cut(s) 326, 636, 911, 1015, 1145, 1285, 1326
Bst6I CTCTTC 1 cut(s) 291
BstBAI YACGTR 1 cut(s) 569
BstBI TTCGAA 1 cut(s) 484
BstC8I GCNNGC 6 cut(s) 375, 524, 688, 800, 874, 1194
BstDEI CTNAG 4 cut(s) 411, 859, 1087, 1096
BstF5I GGATG 3 cut(s) 588, 624, 1283
BstH2I RGCGCY 1 cut(s) 542
BstHHI GCGC 4 cut(s) 301, 541, 804, 1000
BstKTI GATC 7 cut(s) 10, 371, 457, 675, 840, 1266, 1311
BstMAI GTCTC 3 cut(s) 397, 934, 1216
BstMBI GATC 7 cut(s) 7, 368, 454, 672, 837, 1263, 1308
BstMCI CGRYCG 2 cut(s) 627, 1287
BstMWI GCNNNNNNNGC 4 cut(s) 383, 429, 1016, 1083
BstNI CCWGG 3 cut(s) 140, 256, 266
BstNSI RCATGY 3 cut(s) 772, 1047, 1207
BstSCI CCNGG 3 cut(s) 138, 254, 264
BstSFI CTRYAG 1 cut(s) 851
BstV1I GCAGC 1 cut(s) 1110
BstV2I GAAGAC 3 cut(s) 410, 566, 1200
BstX2I RGATCY 2 cut(s) 7, 837
BstYI RGATCY 2 cut(s) 7, 837
BsuI GTATCC 1 cut(s) 899
BsuRI GGCC 3 cut(s) 263, 1103, 1296
BtsCI GGATG 3 cut(s) 588, 624, 1283
BtsI GCAGTG 1 cut(s) 972
BtsIMutI CAGTG 5 cut(s) 916, 928, 972, 1095, 1150
Cac8I GCNNGC 6 cut(s) 375, 524, 688, 800, 874, 1194
CfoI GCGC 4 cut(s) 301, 541, 804, 1000
Cfr13I GGNCC 2 cut(s) 175, 262
Csp6I GTAC 4 cut(s) 632, 669, 1201, 1240
CviAII CATG 7 cut(s) 472, 546, 606, 654, 769, 1044, 1204
CviQI GTAC 4 cut(s) 632, 669, 1201, 1240
DdeI CTNAG 4 cut(s) 411, 859, 1087, 1096
DpnI GATC 7 cut(s) 9, 370, 456, 674, 839, 1265, 1310
DpnII GATC 7 cut(s) 7, 368, 454, 672, 837, 1263, 1308
Eam1104I CTCTTC 1 cut(s) 291
EarI CTCTTC 1 cut(s) 291
EciI GGCGGA 2 cut(s) 20, 785
Eco147I AGGCCT 1 cut(s) 1296
Eco31I GGTCTC 1 cut(s) 397
Eco47I GGWCC 1 cut(s) 175
Eco57I CTGAAG 1 cut(s) 578
EcoRII CCWGG 3 cut(s) 138, 254, 264
Esp3I CGTCTC 1 cut(s) 934
FaeI CATG 7 cut(s) 475, 549, 609, 657, 772, 1047, 1207
FatI CATG 7 cut(s) 471, 545, 605, 653, 768, 1043, 1203
FblI GTMKAC 1 cut(s) 1287
Fnu4HI GCNGC 3 cut(s) 387, 1104, 1124
FokI GGATG 3 cut(s) 595, 631, 1290
Fsp4HI GCNGC 3 cut(s) 387, 1104, 1124
FspBI CTAG 3 cut(s) 194, 356, 873
GlaI GCGC 4 cut(s) 300, 540, 803, 999
GluI GCNGC 3 cut(s) 387, 1104, 1124
HaeII RGCGCY 1 cut(s) 542
HaeIII GGCC 3 cut(s) 263, 1103, 1296
HapII CCGG 2 cut(s) 290, 835
HhaI GCGC 4 cut(s) 301, 541, 804, 1000
Hin1II CATG 7 cut(s) 475, 549, 609, 657, 772, 1047, 1207
Hin6I GCGC 4 cut(s) 299, 539, 802, 998
HinP1I GCGC 4 cut(s) 299, 539, 802, 998
HincII GTYRAC 1 cut(s) 1288
HindII GTYRAC 1 cut(s) 1288
HinfI GANTC 7 cut(s) 415, 512, 562, 602, 761, 886, 1154
HpaII CCGG 2 cut(s) 290, 835
Hpy166II GTNNAC 2 cut(s) 1093, 1288
Hpy188III TCNNGA 2 cut(s) 599, 835
Hpy8I GTNNAC 2 cut(s) 1093, 1288
Hpy99I CGWCG 1 cut(s) 737
HpyAV CCTTC 2 cut(s) 45, 738
HpyCH4III ACNGT 7 cut(s) 326, 636, 911, 1015, 1145, 1285, 1326
HpyCH4IV ACGT 3 cut(s) 200, 568, 1355
HpyCH4V TGCA 9 cut(s) 405, 711, 718, 853, 970, 977, 1047, 1126, 1246
HpyF10VI GCNNNNNNNGC 4 cut(s) 383, 429, 1016, 1083
HpyF3I CTNAG 4 cut(s) 411, 859, 1087, 1096
HpySE526I ACGT 3 cut(s) 200, 568, 1355
Hsp92II CATG 7 cut(s) 475, 549, 609, 657, 772, 1047, 1207
HspAI GCGC 4 cut(s) 299, 539, 802, 998
Kpn2I TCCGGA 1 cut(s) 834
Kzo9I GATC 7 cut(s) 7, 368, 454, 672, 837, 1263, 1308
LguI GCTCTTC 1 cut(s) 291
LmnI GCTCC 5 cut(s) 28, 60, 323, 546, 787
Lsp1109I GCAGC 1 cut(s) 1110
LweI GCATC 1 cut(s) 414
MaeI CTAG 3 cut(s) 194, 356, 873
MaeII ACGT 3 cut(s) 200, 568, 1355
MaeIII GTNAC 5 cut(s) 326, 467, 865, 883, 1133
MalI GATC 7 cut(s) 9, 370, 456, 674, 839, 1265, 1310
MboI GATC 7 cut(s) 7, 368, 454, 672, 837, 1263, 1308
MboII GAAGA 8 cut(s) 161, 164, 222, 278, 410, 571, 832, 1200
MfeI CAATTG 1 cut(s) 170
MflI RGATCY 2 cut(s) 7, 837
MhlI GDGCHC 1 cut(s) 402
MluCI AATT 4 cut(s) 170, 712, 984, 1269
MlyI GAGTC 4 cut(s) 424, 556, 880, 1163
MmeI TCCRAC 2 cut(s) 345, 1164
MroI TCCGGA 1 cut(s) 834
MroXI GAANNNNTTC 1 cut(s) 201
MseI TTAA 5 cut(s) 342, 507, 680, 1037, 1115
MslI CAYNNNNRTG 3 cut(s) 278, 610, 773
MspA1I CMGCKG 1 cut(s) 1192
MspI CCGG 2 cut(s) 290, 835
MspR9I CCNGG 3 cut(s) 140, 256, 266
MunI CAATTG 1 cut(s) 170
Mva1269I GAATGC 2 cut(s) 432, 1036
MvaI CCWGG 3 cut(s) 140, 256, 266
MwoI GCNNNNNNNGC 4 cut(s) 383, 429, 1016, 1083
NdeII GATC 7 cut(s) 7, 368, 454, 672, 837, 1263, 1308
NheI GCTAGC 1 cut(s) 872
NlaIII CATG 7 cut(s) 475, 549, 609, 657, 772, 1047, 1207
NlaIV GGNNCC 3 cut(s) 30, 443, 833
NmuCI GTSAC 4 cut(s) 326, 467, 865, 883
NspI RCATGY 3 cut(s) 772, 1047, 1207
NspV TTCGAA 1 cut(s) 484
PceI AGGCCT 1 cut(s) 1296
PciI ACATGT 1 cut(s) 1203
PciSI GCTCTTC 1 cut(s) 291
PctI GAATGC 2 cut(s) 432, 1036
PdmI GAANNNNTTC 1 cut(s) 201
PfeI GAWTC 3 cut(s) 512, 602, 761
PflMI CCANNNNNTGG 2 cut(s) 139, 1026
PfoI TCCNGGA 1 cut(s) 254
PkrI GCNGC 3 cut(s) 388, 1105, 1125
PleI GAGTC 4 cut(s) 423, 556, 880, 1162
PpsI GAGTC 4 cut(s) 423, 556, 880, 1162
Ppu21I YACGTR 1 cut(s) 569
PscI ACATGT 1 cut(s) 1203
PsiI TTATAA 1 cut(s) 17
Psp1406I AACGTT 1 cut(s) 200
Psp6I CCWGG 3 cut(s) 138, 254, 264
PspGI CCWGG 3 cut(s) 138, 254, 264
PspN4I GGNNCC 3 cut(s) 30, 443, 833
PspPI GGNCC 2 cut(s) 175, 262
PstI CTGCAG 1 cut(s) 855
PsuI RGATCY 2 cut(s) 7, 837
PvuII CAGCTG 1 cut(s) 1192
RsaI GTAC 4 cut(s) 633, 670, 1202, 1241
RsaNI GTAC 4 cut(s) 632, 669, 1201, 1240
RseI CAYNNNNRTG 3 cut(s) 278, 610, 773
SalI GTCGAC 1 cut(s) 1286
SapI GCTCTTC 1 cut(s) 291
SaqAI TTAA 5 cut(s) 342, 507, 680, 1037, 1115
SatI GCNGC 3 cut(s) 387, 1104, 1124
Sau3AI GATC 7 cut(s) 7, 368, 454, 672, 837, 1263, 1308
Sau96I GGNCC 2 cut(s) 175, 262
SchI GAGTC 4 cut(s) 424, 556, 880, 1163
ScrFI CCNGG 3 cut(s) 140, 256, 266
SduI GDGCHC 1 cut(s) 402
SfaNI GCATC 1 cut(s) 414
SfcI CTRYAG 1 cut(s) 851
SfuI TTCGAA 1 cut(s) 484
SinI GGWCC 1 cut(s) 175
SmiMI CAYNNNNRTG 3 cut(s) 278, 610, 773
Sse9I AATT 4 cut(s) 170, 712, 984, 1269
SseBI AGGCCT 1 cut(s) 1296
SsiI CCGC 8 cut(s) 5, 377, 386, 389, 439, 465, 796, 1104
SspMI CTAG 3 cut(s) 194, 356, 873
StuI AGGCCT 1 cut(s) 1296
StyD4I CCNGG 3 cut(s) 138, 254, 264
TaaI ACNGT 7 cut(s) 326, 636, 911, 1015, 1145, 1285, 1326
TaiI ACGT 3 cut(s) 203, 571, 1358
TaqI TCGA 7 cut(s) 351, 484, 732, 1157, 1287, 1320, 1370
TaqII GACCGA 1 cut(s) 613
TasI AATT 4 cut(s) 170, 712, 984, 1269
TatI WGTACW 1 cut(s) 1239
TauI GCSGC 2 cut(s) 389, 1106
TfiI GAWTC 3 cut(s) 512, 602, 761
Tru1I TTAA 5 cut(s) 342, 507, 680, 1037, 1115
Tru9I TTAA 5 cut(s) 342, 507, 680, 1037, 1115
TscAI CASTG 5 cut(s) 916, 928, 979, 1095, 1150
TseFI GTSAC 4 cut(s) 326, 467, 865, 883
TseI GCWGC 1 cut(s) 1123
Tsp45I GTSAC 4 cut(s) 326, 467, 865, 883
TspDTI ATGAA 2 cut(s) 594, 1306
TspRI CASTG 5 cut(s) 916, 928, 979, 1095, 1150
Van91I CCANNNNNTGG 2 cut(s) 139, 1026
VpaK11BI GGWCC 1 cut(s) 175
XapI RAATTY 1 cut(s) 984
XceI RCATGY 3 cut(s) 772, 1047, 1207
XmiI GTMKAC 1 cut(s) 1287
XmnI GAANNNNTTC 1 cut(s) 201
XspI CTAG 3 cut(s) 194, 356, 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.