Rh4AG304300

Protein of unknown function (DUF3537)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0007442
Physical Location & Seq
Forward (+)
61636165 .. 61639847
3683 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG304300.1

Sequence Viewer

Length: 1350 bp
ATGGCTGACCCTCACATTAGAACAGAGGAACGCCCACCTCTCCCACCACTGCCCACCACCCCTCTTCTCGATTCCAGCCAAACCCACCACCCAGAAGATCAAGAGCTCGAAATCAAAGATACCCAGTTGGATCAAACCCTTCAAAGCGTAGAAGCCTTTCTCACTCTGCTGGGTTTCAGCCAATCTTCTCTATTCAGCTTTGTTCTATCTTGGTTTGGTTTTGTAGTCATCGGAGTCTTGCTTCCGGTGCTTGTTCTTGTGATTTTCAACTGTTCTGATTGCGATAAGTACCAGATTGAGGGGTTTGAGCTTGTTATTGTAGGCTCTCAGGCGTGTTTGGCTGCTGTCTCTTTGCTTTGCTTATCTCACAATCTTCGCAAGTATGGCATTCGGAGATTCTTGTTTGTTGATCGATATACTGGCCATATGGTTCGGTTTCGAAACGATTACATCAAGCAGATTAAGGGTTCTTTTCGGTTGCTAATTTGGTGGTCGCTGCCATGTTTGCTGTTGAAGACTGTACGTGAAGTCGCCCGCATGCTCTATGTCCATCATGATTCATGGTGGATGTCATTTTCTATTTTATTAGCTTTTGTTCTATCATGGACATATGCGAGTACAATCACCCTCACAGCCAGCATTTTGTTTCATTTGGTCTGCAATTTGCAAGTAATCCACTTCGAGGATTATGGGAGGCTCTTGGAAAGGGAATCTGATGTTTTGGTATTTATGGAGGAGCACATTCGGCTACGGTATCATCTCTCTAAAATAAGCCACAGGTTCCGGATCTTCCTACTTCTGCAGTTCTTGGTTGTCACAGCAAGCCAGTTTGTGACTCTATTTGAGGTCACTGGATACAGTGGAAAGATCACGGTCATTAATGGTGGCGATTTTGCTGTCTCCACAGTTGTTCAGGTAGTTGGGATTATTATTTCCTTGCATGCAGCGACCAGAATTTCTCATAGAGCCCAGGCCTTAGCATCACTTGCCAGTAGATGGCATGCCTTAGTAACATGTAGTTCTAATGATTCATCTCAGCAAAGAAATTCAAACGGTATGGGGAACTCTGAGGCTGCAAACTCAATTAACTCACTGCATCTCTCTTACTCTGAGAGTGATTTGGAGTCATGTGATTATCATGTTTCAATGCCTACAAATACACAGCTGGCTTCATATATGTCCTCATATCACAGGAGACAAGCTTTTGTAATGTATTTGCAAACTAATGCTGGAGGGATCACAATATTTGGATGGACAGTGGATAGAGCCCTTCTCAACACAATCTTCTTCATCGAACTATCTTTAATTACCTTTGTGCTTGGCAAAACAATAGTTTTCTCATCGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3537

Protein Analysis

449

Amino Acids

50.96

Weight (kDa)

6.65

Isoelectric Point (pI)

43.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3537 PF12056 46 - 431 5.4e-149 Protein of unknown function (DUF3537)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 996
AccIII TCCGGA 1 cut(s) 783
AciI CCGC 1 cut(s) 535
AclWI GGATC 3 cut(s) 138, 794, 1244
AcoI YGGCCR 1 cut(s) 421
AcsI RAATTY 2 cut(s) 954, 1045
AfaI GTAC 3 cut(s) 290, 522, 619
AfiI CCNNNNNNNGG 3 cut(s) 298, 682, 996
AflIII ACRYGT 1 cut(s) 1013
AgsI TTSAA 5 cut(s) 143, 268, 514, 1050, 1146
AjnI CCWGG 1 cut(s) 969
AluBI AGCT 6 cut(s) 106, 198, 310, 590, 1165, 1202
AluI AGCT 6 cut(s) 106, 198, 310, 590, 1165, 1202
Alw21I GWGCWC 2 cut(s) 108, 741
Alw26I GTCTC 3 cut(s) 352, 904, 1189
AlwI GGATC 3 cut(s) 138, 794, 1244
Aor13HI TCCGGA 1 cut(s) 783
AoxI GGCC 2 cut(s) 421, 972
ApeKI GCWGC 4 cut(s) 341, 496, 944, 1073
ApoI RAATTY 2 cut(s) 954, 1045
AseI ATTAAT 1 cut(s) 879
Asp700I GAANNNNTTC 1 cut(s) 156
AsuHPI GGTGA 1 cut(s) 616
AsuII TTCGAA 1 cut(s) 439
BalI TGGCCA 1 cut(s) 423
BanII GRGCYC 3 cut(s) 108, 970, 1270
BbsI GAAGAC 1 cut(s) 521
Bbv12I GWGCWC 2 cut(s) 108, 741
BbvI GCAGC 4 cut(s) 328, 483, 956, 1060
BccI CCATC 3 cut(s) 558, 990, 1245
BciT130I CCWGG 1 cut(s) 971
BciVI GTATCC 1 cut(s) 848
BcoDI GTCTC 3 cut(s) 352, 904, 1189
BfmI CTRYAG 1 cut(s) 800
BfuI GTATCC 1 cut(s) 848
BisI GCNGC 4 cut(s) 342, 497, 945, 1074
BlsI GCNGC 4 cut(s) 343, 498, 946, 1075
Bme1390I CCNGG 1 cut(s) 971
BmiI GGNNCC 1 cut(s) 782
BmrFI CCNGG 1 cut(s) 971
BmrI ACTGGG 1 cut(s) 118
BmsI GCATC 2 cut(s) 989, 1105
BmuI ACTGGG 1 cut(s) 118
BpiI GAAGAC 1 cut(s) 521
BplI GAGNNNNNCTC 2 cut(s) 1257, 1289
BpmI CTGGAG 1 cut(s) 1251
Bpu10I CCTNAGC 1 cut(s) 976
Bpu14I TTCGAA 1 cut(s) 439
Bsa29I ATCGAT 1 cut(s) 412
BsaAI YACGTR 1 cut(s) 524
BsaJI CCNNGG 1 cut(s) 969
BsaWI WCCGGW 2 cut(s) 244, 783
Bsc4I CCNNNNNNNGG 3 cut(s) 298, 682, 996
Bse1I ACTGG 5 cut(s) 124, 424, 826, 856, 990
BseAI TCCGGA 1 cut(s) 783
BseBI CCWGG 1 cut(s) 971
BseCI ATCGAT 1 cut(s) 412
BseDI CCNNGG 1 cut(s) 969
BseGI GGATG 2 cut(s) 573, 1256
BseLI CCNNNNNNNGG 3 cut(s) 298, 682, 996
BseMII CTCAG 4 cut(s) 341, 1049, 1059, 1101
BseNI ACTGG 5 cut(s) 124, 424, 826, 856, 990
BseRI GAGGAG 1 cut(s) 749
BseXI GCAGC 4 cut(s) 328, 483, 956, 1060
BseYI CCCAGC 1 cut(s) 169
BshFI GGCC 2 cut(s) 423, 974
BshVI ATCGAT 1 cut(s) 412
BsiHKAI GWGCWC 2 cut(s) 108, 741
BsiSI CCGG 2 cut(s) 245, 784
BslI CCNNNNNNNGG 3 cut(s) 298, 682, 996
BsmAI GTCTC 3 cut(s) 352, 904, 1189
BsmI GAATGC 1 cut(s) 387
BsnI GGCC 2 cut(s) 423, 974
Bsp119I TTCGAA 1 cut(s) 439
Bsp1286I GDGCHC 4 cut(s) 108, 741, 970, 1270
Bsp13I TCCGGA 1 cut(s) 783
Bsp143I GATC 6 cut(s) 97, 130, 409, 786, 867, 1236
BspACI CCGC 1 cut(s) 535
BspANI GGCC 2 cut(s) 423, 974
BspCNI CTCAG 4 cut(s) 340, 1048, 1060, 1102
BspDI ATCGAT 1 cut(s) 412
BspEI TCCGGA 1 cut(s) 783
BspHI TCATGA 1 cut(s) 553
BspLI GGNNCC 1 cut(s) 782
BspMAI CTGCAG 1 cut(s) 804
BspPI GGATC 3 cut(s) 138, 794, 1244
BspT104I TTCGAA 1 cut(s) 439
BsrI ACTGG 5 cut(s) 124, 424, 826, 856, 990
BssECI CCNNGG 1 cut(s) 969
BssMI GATC 6 cut(s) 97, 130, 409, 786, 867, 1236
Bst2UI CCWGG 1 cut(s) 971
Bst4CI ACNGT 8 cut(s) 272, 520, 753, 860, 874, 907, 1055, 1258
Bst6I CTCTTC 1 cut(s) 69
BstAPI GCANNNNNTGC 1 cut(s) 986
BstBAI YACGTR 1 cut(s) 524
BstBI TTCGAA 1 cut(s) 439
BstC8I GCNNGC 7 cut(s) 535, 539, 637, 823, 942, 1002, 1167
BstDEI CTNAG 6 cut(s) 327, 976, 1006, 1035, 1068, 1110
BstF5I GGATG 2 cut(s) 573, 1256
BstKTI GATC 6 cut(s) 100, 133, 412, 789, 870, 1239
BstMAI GTCTC 3 cut(s) 352, 904, 1189
BstMBI GATC 6 cut(s) 97, 130, 409, 786, 867, 1236
BstMWI GCNNNNNNNGC 6 cut(s) 247, 338, 384, 505, 745, 986
BstNI CCWGG 1 cut(s) 971
BstNSI RCATGY 4 cut(s) 541, 944, 1004, 1017
BstSCI CCNGG 1 cut(s) 969
BstSFI CTRYAG 1 cut(s) 800
BstV1I GCAGC 4 cut(s) 328, 483, 956, 1060
BstV2I GAAGAC 1 cut(s) 521
BstX2I RGATCY 1 cut(s) 786
BstYI RGATCY 1 cut(s) 786
Bsu15I ATCGAT 1 cut(s) 412
BsuI GTATCC 1 cut(s) 848
BsuRI GGCC 2 cut(s) 423, 974
BsuTUI ATCGAT 1 cut(s) 412
BtsCI GGATG 2 cut(s) 573, 1256
BtsI GCAGTG 2 cut(s) 47, 1091
BtsIMutI CAGTG 5 cut(s) 47, 849, 865, 1091, 1263
Cac8I GCNNGC 7 cut(s) 535, 539, 637, 823, 942, 1002, 1167
CciI TCATGA 1 cut(s) 553
ClaI ATCGAT 1 cut(s) 412
Csp6I GTAC 3 cut(s) 289, 521, 618
CviQI GTAC 3 cut(s) 289, 521, 618
DdeI CTNAG 6 cut(s) 327, 976, 1006, 1035, 1068, 1110
DpnI GATC 6 cut(s) 99, 132, 411, 788, 869, 1238
DpnII GATC 6 cut(s) 97, 130, 409, 786, 867, 1236
EaeI YGGCCR 1 cut(s) 421
Eam1104I CTCTTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 69
Ecl136II GAGCTC 1 cut(s) 106
Eco147I AGGCCT 1 cut(s) 974
Eco24I GRGCYC 3 cut(s) 108, 970, 1270
Eco53kI GAGCTC 1 cut(s) 106
EcoICRI GAGCTC 1 cut(s) 106
EcoRII CCWGG 1 cut(s) 969
EcoT38I GRGCYC 3 cut(s) 108, 970, 1270
FauI CCCGC 1 cut(s) 542
FauNDI CATATG 2 cut(s) 426, 610
Fnu4HI GCNGC 4 cut(s) 342, 497, 945, 1074
FokI GGATG 2 cut(s) 580, 1263
FriOI GRGCYC 3 cut(s) 108, 970, 1270
Fsp4HI GCNGC 4 cut(s) 342, 497, 945, 1074
GluI GCNGC 4 cut(s) 342, 497, 945, 1074
GsaI CCCAGC 1 cut(s) 173
GsuI CTGGAG 1 cut(s) 1251
HaeIII GGCC 2 cut(s) 423, 974
HapII CCGG 2 cut(s) 245, 784
HindIII AAGCTT 1 cut(s) 1200
HinfI GANTC 8 cut(s) 71, 234, 396, 557, 710, 835, 1028, 1124
HpaII CCGG 2 cut(s) 245, 784
HphI GGTGA 1 cut(s) 616
Hpy188I TCNGA 6 cut(s) 233, 277, 393, 715, 1069, 1111
Hpy188III TCNNGA 4 cut(s) 68, 101, 554, 784
HpyAV CCTTC 2 cut(s) 149, 1280
HpyCH4III ACNGT 8 cut(s) 272, 520, 753, 860, 874, 907, 1055, 1258
HpyCH4IV ACGT 1 cut(s) 523
HpyCH4V TGCA 8 cut(s) 660, 667, 802, 940, 944, 1076, 1096, 1219
HpyF10VI GCNNNNNNNGC 6 cut(s) 247, 338, 384, 505, 745, 986
HpyF3I CTNAG 6 cut(s) 327, 976, 1006, 1035, 1068, 1110
HpySE526I ACGT 1 cut(s) 523
Kpn2I TCCGGA 1 cut(s) 783
Kzo9I GATC 6 cut(s) 97, 130, 409, 786, 867, 1236
LmnI GCTCC 1 cut(s) 736
Lsp1109I GCAGC 4 cut(s) 328, 483, 956, 1060
LweI GCATC 2 cut(s) 989, 1105
MaeII ACGT 1 cut(s) 523
MaeIII GTNAC 4 cut(s) 814, 832, 847, 1009
MalI GATC 6 cut(s) 99, 132, 411, 788, 869, 1238
MboI GATC 6 cut(s) 97, 130, 409, 786, 867, 1236
MboII GAAGA 8 cut(s) 56, 107, 177, 365, 526, 781, 1276, 1279
MflI RGATCY 1 cut(s) 786
MhlI GDGCHC 4 cut(s) 108, 741, 970, 1270
MlsI TGGCCA 1 cut(s) 423
MluCI AATT 6 cut(s) 483, 661, 954, 1045, 1083, 1305
MluNI TGGCCA 1 cut(s) 423
MlyI GAGTC 3 cut(s) 243, 829, 1133
MmeI TCCRAC 1 cut(s) 108
Mox20I TGGCCA 1 cut(s) 423
MroI TCCGGA 1 cut(s) 783
MroXI GAANNNNTTC 1 cut(s) 156
MscI TGGCCA 1 cut(s) 423
MseI TTAA 4 cut(s) 462, 879, 1086, 1304
Msp20I TGGCCA 1 cut(s) 423
MspA1I CMGCKG 1 cut(s) 1165
MspI CCGG 2 cut(s) 245, 784
MspR9I CCNGG 1 cut(s) 971
Mva1269I GAATGC 1 cut(s) 387
MvaI CCWGG 1 cut(s) 971
MwoI GCNNNNNNNGC 6 cut(s) 247, 338, 384, 505, 745, 986
NdeI CATATG 2 cut(s) 426, 610
NdeII GATC 6 cut(s) 97, 130, 409, 786, 867, 1236
NlaIV GGNNCC 1 cut(s) 782
NmuCI GTSAC 3 cut(s) 814, 832, 847
NspI RCATGY 4 cut(s) 541, 944, 1004, 1017
NspV TTCGAA 1 cut(s) 439
PaeI GCATGC 3 cut(s) 541, 944, 1004
PagI TCATGA 1 cut(s) 553
PceI AGGCCT 1 cut(s) 974
PciI ACATGT 1 cut(s) 1013
PctI GAATGC 1 cut(s) 387
PdmI GAANNNNTTC 1 cut(s) 156
PfeI GAWTC 5 cut(s) 71, 396, 557, 710, 1028
PflMI CCANNNNNTGG 1 cut(s) 996
PkrI GCNGC 4 cut(s) 343, 498, 946, 1075
PleI GAGTC 3 cut(s) 242, 829, 1132
PpsI GAGTC 3 cut(s) 242, 829, 1132
Ppu21I YACGTR 1 cut(s) 524
PscI ACATGT 1 cut(s) 1013
PshBI ATTAAT 1 cut(s) 879
Psp124BI GAGCTC 1 cut(s) 108
Psp6I CCWGG 1 cut(s) 969
PspFI CCCAGC 1 cut(s) 169
PspGI CCWGG 1 cut(s) 969
PspN4I GGNNCC 1 cut(s) 782
PstI CTGCAG 1 cut(s) 804
PsuI RGATCY 1 cut(s) 786
PvuII CAGCTG 1 cut(s) 1165
RsaI GTAC 3 cut(s) 290, 522, 619
RsaNI GTAC 3 cut(s) 289, 521, 618
SacI GAGCTC 1 cut(s) 108
SaqAI TTAA 4 cut(s) 462, 879, 1086, 1304
SatI GCNGC 4 cut(s) 342, 497, 945, 1074
Sau3AI GATC 6 cut(s) 97, 130, 409, 786, 867, 1236
SchI GAGTC 3 cut(s) 243, 829, 1133
ScrFI CCNGG 1 cut(s) 971
SduI GDGCHC 4 cut(s) 108, 741, 970, 1270
SfaNI GCATC 2 cut(s) 989, 1105
SfcI CTRYAG 1 cut(s) 800
SfuI TTCGAA 1 cut(s) 439
SphI GCATGC 3 cut(s) 541, 944, 1004
Sse9I AATT 6 cut(s) 483, 661, 954, 1045, 1083, 1305
SseBI AGGCCT 1 cut(s) 974
SsiI CCGC 1 cut(s) 535
SspI AATATT 1 cut(s) 1245
SstI GAGCTC 1 cut(s) 108
StuI AGGCCT 1 cut(s) 974
StyD4I CCNGG 1 cut(s) 969
TaaI ACNGT 8 cut(s) 272, 520, 753, 860, 874, 907, 1055, 1258
TaiI ACGT 1 cut(s) 526
TaqI TCGA 7 cut(s) 69, 108, 412, 439, 681, 1293, 1343
TasI AATT 6 cut(s) 483, 661, 954, 1045, 1083, 1305
TatI WGTACW 1 cut(s) 617
TfiI GAWTC 5 cut(s) 71, 396, 557, 710, 1028
Tru1I TTAA 4 cut(s) 462, 879, 1086, 1304
Tru9I TTAA 4 cut(s) 462, 879, 1086, 1304
TscAI CASTG 5 cut(s) 54, 856, 865, 1098, 1263
TseFI GTSAC 3 cut(s) 814, 832, 847
TseI GCWGC 4 cut(s) 341, 496, 944, 1073
Tsp45I GTSAC 3 cut(s) 814, 832, 847
TspDTI ATGAA 5 cut(s) 549, 638, 1020, 1161, 1279
TspRI CASTG 5 cut(s) 54, 856, 865, 1098, 1263
Van91I CCANNNNNTGG 1 cut(s) 996
VspI ATTAAT 1 cut(s) 879
XapI RAATTY 2 cut(s) 954, 1045
XceI RCATGY 4 cut(s) 541, 944, 1004, 1017
XmnI GAANNNNTTC 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.