MD13G1113900.v1.1

Protein of unknown function (DUF3537)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007442
Physical Location & Seq
Reverse (-)
8261668 .. 8264177
2510 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1113900.v1.1.491

Sequence Viewer

Length: 1332 bp
ATGGCGGACCCTCATATAAAAACAGATGAGCCACCTTCCCAGCTTACCCTTCTTCTCGATTCAAATCACAACAACCGCCACCAAGCACCAGAAGGAGAATACAAAGACACCCAATTGGACCAAACCCTCCAACGGCTAGAAACGTTTCTCACTCTATTGGGCTTCGATCAATCCTCTTGGTTCAGCCTTGCGCTGTCCTGGACTGCTCTGGCTTTTCTCGGTGTGGTGCTTCCGGTGGTCGTGCTCGAGATGTTCAACTGCTCGGACTGCGAGAAGTATCAGGTAAAAGGGTTCGAGCTCGTTATCGTGGGCTCGCAGGCGGTTCTGGCGGCGGTCTCTTTGCTCTGCATCTCTCATTGTCTTCGCAAGTATGGCATTCGGAGGTTCCTCTTTGTCGATCGCTATAGTGGCCGTATGGTTCGGTTTCGAAACGATTACATCAGGCAGATTAAGGGTTCTTTACGCCTGCTCATTTGGTGGGCGCTGCCGTGTTTCCTTTTGAAGACTCTGCGTGAGGTCATCCGCATGATATATGTCCATCAGTCATGGTGGCTGTCATTTGCTGTATTACTAGCTTTGGTTCTGTCATGGGCTTATGTGAGTACAATCACTCTAACAGCCAGCATTTTATTTCACTTGATCTGCAACTTGCAAGTTATCCACTTCGATGATTATGGAAGGCTCTTGGAAAGGGATTCTAATGTGTTGCTATTTATGGAGGAGCATATTCGTCTGCGGTATCATCTTCATAAAATAAGCCACAGGTTCCGGATCTTCCTTCTTCTGCAGTTTATAATCGTCACCACGAGCCAGTTTGTGACTCTATTTGAGATCACAATATACAGTGGAAAAATCACTGTCATAAATGGCGGCGATTTTGCAGTCTCCACAATTGTTCAGGTTGTCGGCATTATTCTTTCCTTGCACGCAGCTACTAAAATTTCTGGTAGAGCCCAAGGTATAGCGACAGTTGCCAGTAGATGGCATGCGTTAATGACATGCAGTTCTAATGATTCACCGCATAACAGAAGTTCAGACAGTTTGGTGAACTTTGAGGCTGCCAACAGATTGAGCTCACTGCATTCAAGTTACTCTGAGAGTGATTTGGAGTCGGCGGATTATGTTCATGTGCCTACAAATTCCCAGCTGGCTTCACATATGGCTTCATACCACAAGAGACAAGCTTTTGTGTTATACTTGCAAACCAATCATGGGGGCATAACAATTTTCGGATGGATGGTTGATAGAGGCCTCCTCACCACGATATTTTTCATCGAACTGTCTTTGATTACCTTTGTTCTTGGACAGACAATAGTTTTTTCGGCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3537

Protein Analysis

444

Amino Acids

50.43

Weight (kDa)

8.14

Isoelectric Point (pI)

35.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3537 PF12056 42 - 425 1e-143 Protein of unknown function (DUF3537)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 794
AccB7I CCANNNNNTGG 1 cut(s) 981
AccIII TCCGGA 1 cut(s) 768
AclI AACGTT 1 cut(s) 143
AclWI GGATC 1 cut(s) 779
AcoI YGGCCR 1 cut(s) 409
AcsI RAATTY 2 cut(s) 939, 1138
AfaI GTAC 1 cut(s) 604
AfiI CCNNNNNNNGG 3 cut(s) 132, 981, 1212
AgsI TTSAA 4 cut(s) 63, 256, 502, 1086
AjnI CCWGG 1 cut(s) 197
AluBI AGCT 7 cut(s) 43, 298, 575, 932, 1074, 1147, 1184
AluI AGCT 7 cut(s) 43, 298, 575, 932, 1074, 1147, 1184
Alw21I GWGCWC 3 cut(s) 246, 300, 1076
Alw26I GTCTC 3 cut(s) 340, 889, 1171
AlwI GGATC 1 cut(s) 779
Ama87I CYCGRG 1 cut(s) 245
Aor13HI TCCGGA 1 cut(s) 768
AoxI GGCC 2 cut(s) 409, 1249
ApeKI GCWGC 4 cut(s) 484, 929, 1058, 1325
ApoI RAATTY 2 cut(s) 939, 1138
Asp700I GAANNNNTTC 1 cut(s) 144
AspLEI GCGC 2 cut(s) 193, 484
AspS9I GGNCC 2 cut(s) 7, 118
AsuHPI GGTGA 4 cut(s) 793, 1008, 1057, 1249
AsuII TTCGAA 1 cut(s) 427
AvaI CYCGRG 1 cut(s) 245
AvaII GGWCC 2 cut(s) 7, 118
BanII GRGCYC 4 cut(s) 300, 314, 955, 1076
BauI CACGAG 1 cut(s) 805
BbsI GAAGAC 2 cut(s) 353, 509
Bbv12I GWGCWC 3 cut(s) 246, 300, 1076
BbvI GCAGC 4 cut(s) 471, 941, 1045, 1312
BccI CCATC 4 cut(s) 546, 975, 1227, 1231
BceAI ACGGC 3 cut(s) 149, 396, 472
BciT130I CCWGG 1 cut(s) 199
BcoDI GTCTC 3 cut(s) 340, 889, 1171
BfaI CTAG 2 cut(s) 137, 572
BfmI CTRYAG 2 cut(s) 403, 785
BfoI RGCGCY 1 cut(s) 485
BisI GCNGC 6 cut(s) 330, 485, 871, 930, 1059, 1326
BlsI GCNGC 6 cut(s) 331, 486, 872, 931, 1060, 1327
Bme1390I CCNGG 1 cut(s) 199
Bme18I GGWCC 2 cut(s) 7, 118
BmeT110I CYCGRG 1 cut(s) 245
BmgT120I GGNCC 2 cut(s) 7, 118
BmiI GGNNCC 3 cut(s) 9, 386, 767
BmrFI CCNGG 1 cut(s) 199
BmsI GCATC 1 cut(s) 357
BpiI GAAGAC 2 cut(s) 353, 509
BplI GAGNNNNNCTC 2 cut(s) 1239, 1271
Bpu14I TTCGAA 1 cut(s) 427
BsaBI GATNNNNATC 1 cut(s) 63
BsaI GGTCTC 1 cut(s) 340
BsaJI CCNNGG 1 cut(s) 955
BsaWI WCCGGW 2 cut(s) 232, 768
Bsc4I CCNNNNNNNGG 3 cut(s) 132, 981, 1212
Bse1I ACTGG 2 cut(s) 811, 975
Bse8I GATNNNNATC 1 cut(s) 63
BseAI TCCGGA 1 cut(s) 768
BseBI CCWGG 1 cut(s) 199
BseDI CCNNGG 1 cut(s) 955
BseGI GGATG 3 cut(s) 519, 1238, 1242
BseJI GATNNNNATC 1 cut(s) 63
BseLI CCNNNNNNNGG 3 cut(s) 132, 981, 1212
BseMII CTCAG 1 cut(s) 1086
BseNI ACTGG 2 cut(s) 811, 975
BseRI GAGGAG 2 cut(s) 734, 1244
BseXI GCAGC 4 cut(s) 471, 941, 1045, 1312
BseYI CCCAGC 2 cut(s) 39, 1143
Bsh1285I CGRYCG 1 cut(s) 400
BshFI GGCC 2 cut(s) 411, 1251
BsiEI CGRYCG 1 cut(s) 400
BsiHKAI GWGCWC 3 cut(s) 246, 300, 1076
BsiHKCI CYCGRG 1 cut(s) 245
BsiSI CCGG 2 cut(s) 233, 769
BslI CCNNNNNNNGG 3 cut(s) 132, 981, 1212
BsmAI GTCTC 3 cut(s) 340, 889, 1171
BsmI GAATGC 2 cut(s) 375, 1081
BsnI GGCC 2 cut(s) 411, 1251
Bso31I GGTCTC 1 cut(s) 340
BsoBI CYCGRG 1 cut(s) 245
Bsp119I TTCGAA 1 cut(s) 427
Bsp1286I GDGCHC 5 cut(s) 246, 300, 314, 955, 1076
Bsp13I TCCGGA 1 cut(s) 768
Bsp143I GATC 5 cut(s) 166, 397, 639, 771, 831
BspANI GGCC 2 cut(s) 411, 1251
BspCNI CTCAG 1 cut(s) 1087
BspEI TCCGGA 1 cut(s) 768
BspLI GGNNCC 3 cut(s) 9, 386, 767
BspMAI CTGCAG 1 cut(s) 789
BspPI GGATC 1 cut(s) 779
BspT104I TTCGAA 1 cut(s) 427
BspTNI GGTCTC 1 cut(s) 340
BsrI ACTGG 2 cut(s) 811, 975
BssECI CCNNGG 1 cut(s) 955
BssMI GATC 5 cut(s) 166, 397, 639, 771, 831
BssSI CACGAG 1 cut(s) 805
BssT1I CCWWGG 1 cut(s) 955
Bst2BI CACGAG 1 cut(s) 805
Bst2UI CCWGG 1 cut(s) 199
Bst4CI ACNGT 5 cut(s) 845, 859, 970, 1040, 1281
BstBI TTCGAA 1 cut(s) 427
BstC8I GCNNGC 7 cut(s) 314, 318, 467, 622, 927, 987, 1149
BstDEI CTNAG 1 cut(s) 1095
BstF5I GGATG 3 cut(s) 519, 1238, 1242
BstH2I RGCGCY 1 cut(s) 485
BstHHI GCGC 2 cut(s) 193, 484
BstKTI GATC 5 cut(s) 169, 400, 642, 774, 834
BstMAI GTCTC 3 cut(s) 340, 889, 1171
BstMBI GATC 5 cut(s) 166, 397, 639, 771, 831
BstMCI CGRYCG 1 cut(s) 400
BstMWI GCNNNNNNNGC 5 cut(s) 267, 326, 372, 408, 971
BstNI CCWGG 1 cut(s) 199
BstNSI RCATGY 2 cut(s) 989, 1002
BstSCI CCNGG 1 cut(s) 197
BstSFI CTRYAG 2 cut(s) 403, 785
BstV1I GCAGC 4 cut(s) 471, 941, 1045, 1312
BstV2I GAAGAC 2 cut(s) 353, 509
BstX2I RGATCY 1 cut(s) 771
BstYI RGATCY 1 cut(s) 771
BsuRI GGCC 2 cut(s) 411, 1251
BtsCI GGATG 3 cut(s) 519, 1238, 1242
BtsI GCAGTG 1 cut(s) 1076
BtsIMutI CAGTG 3 cut(s) 850, 855, 1076
Cac8I GCNNGC 7 cut(s) 314, 318, 467, 622, 927, 987, 1149
CfoI GCGC 2 cut(s) 193, 484
Cfr13I GGNCC 2 cut(s) 7, 118
Csp6I GTAC 1 cut(s) 603
CviAII CATG 7 cut(s) 526, 546, 588, 986, 999, 1127, 1211
CviQI GTAC 1 cut(s) 603
DdeI CTNAG 1 cut(s) 1095
DpnI GATC 5 cut(s) 168, 399, 641, 773, 833
DpnII GATC 5 cut(s) 166, 397, 639, 771, 831
EaeI YGGCCR 1 cut(s) 409
EciI GGCGGA 2 cut(s) 20, 1130
Ecl136II GAGCTC 2 cut(s) 298, 1074
Eco130I CCWWGG 1 cut(s) 955
Eco147I AGGCCT 1 cut(s) 1251
Eco24I GRGCYC 4 cut(s) 300, 314, 955, 1076
Eco31I GGTCTC 1 cut(s) 340
Eco47I GGWCC 2 cut(s) 7, 118
Eco53kI GAGCTC 2 cut(s) 298, 1074
Eco88I CYCGRG 1 cut(s) 245
EcoICRI GAGCTC 2 cut(s) 298, 1074
EcoRII CCWGG 1 cut(s) 197
EcoT14I CCWWGG 1 cut(s) 955
EcoT38I GRGCYC 4 cut(s) 300, 314, 955, 1076
ErhI CCWWGG 1 cut(s) 955
FaeI CATG 7 cut(s) 529, 549, 591, 989, 1002, 1130, 1214
FatI CATG 7 cut(s) 525, 545, 587, 985, 998, 1126, 1210
FauNDI CATATG 1 cut(s) 1158
Fnu4HI GCNGC 6 cut(s) 330, 485, 871, 930, 1059, 1326
FokI GGATG 3 cut(s) 506, 1245, 1249
FriOI GRGCYC 4 cut(s) 300, 314, 955, 1076
Fsp4HI GCNGC 6 cut(s) 330, 485, 871, 930, 1059, 1326
FspBI CTAG 2 cut(s) 137, 572
GlaI GCGC 2 cut(s) 192, 483
GluI GCNGC 6 cut(s) 330, 485, 871, 930, 1059, 1326
GsaI CCCAGC 2 cut(s) 43, 1147
HaeII RGCGCY 1 cut(s) 485
HaeIII GGCC 2 cut(s) 411, 1251
HapII CCGG 2 cut(s) 233, 769
HhaI GCGC 2 cut(s) 193, 484
Hin1II CATG 7 cut(s) 529, 549, 591, 989, 1002, 1130, 1214
Hin6I GCGC 2 cut(s) 191, 482
HinP1I GCGC 2 cut(s) 191, 482
HindIII AAGCTT 1 cut(s) 1182
HinfI GANTC 6 cut(s) 59, 505, 695, 820, 1013, 1109
HpaII CCGG 2 cut(s) 233, 769
HphI GGTGA 4 cut(s) 793, 1008, 1057, 1249
Hpy166II GTNNAC 1 cut(s) 1048
Hpy188I TCNGA 5 cut(s) 265, 381, 1036, 1096, 1232
Hpy188III TCNNGA 3 cut(s) 56, 247, 769
Hpy8I GTNNAC 1 cut(s) 1048
HpyAV CCTTC 5 cut(s) 45, 59, 86, 672, 788
HpyCH4III ACNGT 5 cut(s) 845, 859, 970, 1040, 1281
HpyCH4IV ACGT 1 cut(s) 143
HpyCH4V TGCA 9 cut(s) 348, 645, 652, 787, 881, 925, 1002, 1081, 1201
HpyF10VI GCNNNNNNNGC 5 cut(s) 267, 326, 372, 408, 971
HpyF3I CTNAG 1 cut(s) 1095
HpySE526I ACGT 1 cut(s) 143
Hsp92II CATG 7 cut(s) 529, 549, 591, 989, 1002, 1130, 1214
HspAI GCGC 2 cut(s) 191, 482
Kpn2I TCCGGA 1 cut(s) 768
Kzo9I GATC 5 cut(s) 166, 397, 639, 771, 831
LmnI GCTCC 1 cut(s) 721
Lsp1109I GCAGC 4 cut(s) 471, 941, 1045, 1312
LweI GCATC 1 cut(s) 357
MaeI CTAG 2 cut(s) 137, 572
MaeII ACGT 1 cut(s) 143
MaeIII GTNAC 3 cut(s) 799, 817, 1088
MalI GATC 5 cut(s) 168, 399, 641, 773, 833
MboI GATC 5 cut(s) 166, 397, 639, 771, 831
MboII GAAGA 6 cut(s) 44, 353, 514, 737, 766, 773
MfeI CAATTG 2 cut(s) 113, 891
MflI RGATCY 1 cut(s) 771
MhlI GDGCHC 5 cut(s) 246, 300, 314, 955, 1076
MluCI AATT 5 cut(s) 113, 891, 939, 1138, 1224
MlyI GAGTC 3 cut(s) 499, 814, 1118
MmeI TCCRAC 1 cut(s) 154
MroI TCCGGA 1 cut(s) 768
MroXI GAANNNNTTC 1 cut(s) 144
MseI TTAA 2 cut(s) 450, 992
MslI CAYNNNNRTG 2 cut(s) 524, 666
MspA1I CMGCKG 1 cut(s) 1147
MspI CCGG 2 cut(s) 233, 769
MspR9I CCNGG 1 cut(s) 199
MunI CAATTG 2 cut(s) 113, 891
Mva1269I GAATGC 2 cut(s) 375, 1081
MvaI CCWGG 1 cut(s) 199
MwoI GCNNNNNNNGC 5 cut(s) 267, 326, 372, 408, 971
NdeI CATATG 1 cut(s) 1158
NdeII GATC 5 cut(s) 166, 397, 639, 771, 831
NlaIII CATG 7 cut(s) 529, 549, 591, 989, 1002, 1130, 1214
NlaIV GGNNCC 3 cut(s) 9, 386, 767
NmuCI GTSAC 2 cut(s) 799, 817
NspI RCATGY 2 cut(s) 989, 1002
NspV TTCGAA 1 cut(s) 427
PaeI GCATGC 1 cut(s) 989
PaeR7I CTCGAG 1 cut(s) 245
PceI AGGCCT 1 cut(s) 1251
PctI GAATGC 2 cut(s) 375, 1081
PdmI GAANNNNTTC 1 cut(s) 144
PfeI GAWTC 3 cut(s) 59, 695, 1013
PflMI CCANNNNNTGG 1 cut(s) 981
PfoI TCCNGGA 1 cut(s) 197
PkrI GCNGC 6 cut(s) 331, 486, 872, 931, 1060, 1327
Ple19I CGATCG 1 cut(s) 400
PleI GAGTC 3 cut(s) 499, 814, 1117
PpsI GAGTC 3 cut(s) 499, 814, 1117
PsiI TTATAA 1 cut(s) 794
Psp124BI GAGCTC 2 cut(s) 300, 1076
Psp1406I AACGTT 1 cut(s) 143
Psp6I CCWGG 1 cut(s) 197
PspFI CCCAGC 2 cut(s) 39, 1143
PspGI CCWGG 1 cut(s) 197
PspN4I GGNNCC 3 cut(s) 9, 386, 767
PspPI GGNCC 2 cut(s) 7, 118
PstI CTGCAG 1 cut(s) 789
PsuI RGATCY 1 cut(s) 771
PvuI CGATCG 1 cut(s) 400
PvuII CAGCTG 1 cut(s) 1147
RsaI GTAC 1 cut(s) 604
RsaNI GTAC 1 cut(s) 603
RseI CAYNNNNRTG 2 cut(s) 524, 666
SacI GAGCTC 2 cut(s) 300, 1076
SaqAI TTAA 2 cut(s) 450, 992
SatI GCNGC 6 cut(s) 330, 485, 871, 930, 1059, 1326
Sau3AI GATC 5 cut(s) 166, 397, 639, 771, 831
Sau96I GGNCC 2 cut(s) 7, 118
SchI GAGTC 3 cut(s) 499, 814, 1118
ScrFI CCNGG 1 cut(s) 199
SduI GDGCHC 5 cut(s) 246, 300, 314, 955, 1076
SfaNI GCATC 1 cut(s) 357
SfcI CTRYAG 2 cut(s) 403, 785
Sfr274I CTCGAG 1 cut(s) 245
SfuI TTCGAA 1 cut(s) 427
SinI GGWCC 2 cut(s) 7, 118
SlaI CTCGAG 1 cut(s) 245
SmiMI CAYNNNNRTG 2 cut(s) 524, 666
SmlI CTYRAG 1 cut(s) 245
SmoI CTYRAG 1 cut(s) 245
SphI GCATGC 1 cut(s) 989
Sse9I AATT 5 cut(s) 113, 891, 939, 1138, 1224
SseBI AGGCCT 1 cut(s) 1251
SspMI CTAG 2 cut(s) 137, 572
SstI GAGCTC 2 cut(s) 300, 1076
StuI AGGCCT 1 cut(s) 1251
StyD4I CCNGG 1 cut(s) 197
StyI CCWWGG 1 cut(s) 955
TaaI ACNGT 5 cut(s) 845, 859, 970, 1040, 1281
TaiI ACGT 1 cut(s) 146
TaqI TCGA 8 cut(s) 57, 165, 246, 294, 396, 427, 666, 1275
TasI AATT 5 cut(s) 113, 891, 939, 1138, 1224
TatI WGTACW 1 cut(s) 602
TauI GCSGC 2 cut(s) 332, 873
TfiI GAWTC 3 cut(s) 59, 695, 1013
Tru1I TTAA 2 cut(s) 450, 992
Tru9I TTAA 2 cut(s) 450, 992
TscAI CASTG 3 cut(s) 850, 862, 1083
TseFI GTSAC 2 cut(s) 799, 817
TseI GCWGC 4 cut(s) 484, 929, 1058, 1325
Tsp45I GTSAC 2 cut(s) 799, 817
TspDTI ATGAA 4 cut(s) 737, 1115, 1155, 1261
TspRI CASTG 3 cut(s) 850, 862, 1083
Van91I CCANNNNNTGG 1 cut(s) 981
VpaK11BI GGWCC 2 cut(s) 7, 118
XapI RAATTY 2 cut(s) 939, 1138
XceI RCATGY 2 cut(s) 989, 1002
XhoI CTCGAG 1 cut(s) 245
XmnI GAANNNNTTC 1 cut(s) 144
XspI CTAG 2 cut(s) 137, 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.