MD16G1113100.v1.1

Protein of unknown function (DUF3537)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007442
Physical Location & Seq
Reverse (-)
7921794 .. 7925234
3441 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1113100.v1.1.491

Sequence Viewer

Length: 1332 bp
ATGGCGGACCCTCTAAAACGAGAGGATCCGCCTTCCGAACTCGCCCTTCTTCTCGATTCAAATCACAACAACCGTCACCAAGCACCAGAAGGAGAATGCAAGGATACTCAATTGGACCAGACCCTCCAACGGCTAGAAACGTTTCTCACTCTATTGGGCTTCGGTCAATCTTCTTGGTCGAGCCTTGCGCTGTCCTGGACGGCTCTGGTTCTTCTTGGTGTTGTTCTCCCGGTGGTGGTGCTAGAGATATTCAACTGTTCGGACTGCGACAAGTATCAGGTTAAAGGGTTCGAGCTCAGTATCGTGGCATCGCAGGCGGTTCTGGCGGCGGTCTCTTTGCTCTGCATCTCTCACTGTCTTCGCAAGTATGGCATTCGGAGGTTCCTCTTTGTTGATCGCTATAGTGGCCGTATGGTTCGGTTTCGAAACGATTACATCAAGCAGATTAAGGGTTCTTTACGCCTGCTCATTTGGTGGGCGCTGCCATGTTTTCTTTTGAAAACTCTGCGAGAGGTCATCCGCATGATATCTGTCCATCACAAGTCATGGTGGATGTCATTTGCTGTATTAGTAGCTTTGGTTCTATCATGGGCTTATGTGAGTACAATCACTCTAACAGCCAGCATTTTGTTTCACTTGGTCTGCAACTTGCAAGTTATCCACTTCGATGATTATGGAAGGCTCTTCGAAAGGGATTCTAATGTGTTGCTATTTATGGAGGAGCATATTCGCCTGCGGTATCATCTCCATAAAATAAGCCATAGGTTCCGGATGTTCCTTCTTGTGCAGTTTATAATCGTCACCACAAGCCAGTTTGTGACTCTACTCGAGCTCACAATATACAGTGGAAAAATCACTGTCATAAACAGTGGCGATTTTGCAGTCTCCACAATTGTTCAGGTTGTTGGCATTACTATTTCCCTGCATGCAGCTACTAAAATTTCTGGTAGAGCCCAAGGTATAACGACAGTTGCCAGTAGATGGCATGCGTTAATGACATGCAGTTCTAACAATTCATCTCACAACAGAAGTTCAGACAGTCTGGTGAACTTTGAGGCCGCCAACAGATTGAGTTCACTGCATCTAAGTTACTCCGAGAGTGATTTGGAGTCAGCAGATTATGTTCACGTGCCTACAAATTCCCAGCTGGCTTCACATATGGCTTCATATCACAAGAGACAAGCTTTTGTGTTATACTTGCAAACCAATCACGGGGGGATAACGATTTTCGGATGGATGGTCGATAGAGACCTCCTCACCACGATCTTTTTCATCGAACTATCTCTGATTACCTTTGTTCTTGGTCAGACAATAGCTTTTTCGTCCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3537

Protein Analysis

444

Amino Acids

50.27

Weight (kDa)

8.57

Isoelectric Point (pI)

35.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3537 PF12056 41 - 425 4.4e-143 Protein of unknown function (DUF3537)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 794
AccB7I CCANNNNNTGG 1 cut(s) 981
AccIII TCCGGA 1 cut(s) 768
AciI CCGC 8 cut(s) 5, 29, 317, 326, 329, 520, 736, 1059
AclI AACGTT 1 cut(s) 140
AclWI GGATC 2 cut(s) 20, 33
AcoI YGGCCR 1 cut(s) 406
AcsI RAATTY 2 cut(s) 939, 1138
AcvI CACGTG 1 cut(s) 1129
AfaI GTAC 1 cut(s) 604
AfiI CCNNNNNNNGG 4 cut(s) 129, 235, 981, 1212
AgsI TTSAA 3 cut(s) 60, 253, 499
AjnI CCWGG 1 cut(s) 194
AluBI AGCT 7 cut(s) 295, 575, 832, 932, 1147, 1184, 1316
AluI AGCT 7 cut(s) 295, 575, 832, 932, 1147, 1184, 1316
Alw21I GWGCWC 2 cut(s) 297, 834
Alw26I GTCTC 4 cut(s) 337, 889, 1171, 1242
AlwI GGATC 2 cut(s) 20, 33
Ama87I CYCGRG 1 cut(s) 827
Aor13HI TCCGGA 1 cut(s) 768
AoxI GGCC 2 cut(s) 406, 1056
ApeKI GCWGC 2 cut(s) 481, 929
ApoI RAATTY 2 cut(s) 939, 1138
Asp700I GAANNNNTTC 1 cut(s) 141
AspLEI GCGC 2 cut(s) 190, 481
AspS9I GGNCC 2 cut(s) 7, 115
AsuC2I CCSGG 1 cut(s) 230
AsuHPI GGTGA 4 cut(s) 68, 793, 1057, 1249
AsuII TTCGAA 2 cut(s) 424, 687
AvaI CYCGRG 1 cut(s) 827
AvaII GGWCC 2 cut(s) 7, 115
BamHI GGATCC 1 cut(s) 25
BanII GRGCYC 3 cut(s) 297, 834, 955
BbrPI CACGTG 1 cut(s) 1129
BbsI GAAGAC 1 cut(s) 350
Bbv12I GWGCWC 2 cut(s) 297, 834
BbvI GCAGC 2 cut(s) 468, 941
BccI CCATC 4 cut(s) 543, 975, 1227, 1231
BceAI ACGGC 3 cut(s) 146, 216, 393
BciT130I CCWGG 1 cut(s) 196
BciVI GTATCC 1 cut(s) 97
BcnI CCSGG 1 cut(s) 230
BcoDI GTCTC 4 cut(s) 337, 889, 1171, 1242
BfaI CTAG 2 cut(s) 134, 242
BfmI CTRYAG 1 cut(s) 400
BfoI RGCGCY 1 cut(s) 482
BfuI GTATCC 1 cut(s) 97
BisI GCNGC 4 cut(s) 327, 482, 930, 1059
BlsI GCNGC 4 cut(s) 328, 483, 931, 1060
Bme1390I CCNGG 2 cut(s) 196, 230
Bme18I GGWCC 2 cut(s) 7, 115
BmeT110I CYCGRG 1 cut(s) 827
BmgT120I GGNCC 2 cut(s) 7, 115
BmiI GGNNCC 4 cut(s) 9, 27, 383, 767
BmrFI CCNGG 2 cut(s) 196, 230
BmsI GCATC 3 cut(s) 317, 354, 1090
BpiI GAAGAC 1 cut(s) 350
BplI GAGNNNNNCTC 2 cut(s) 1239, 1271
Bpu14I TTCGAA 2 cut(s) 424, 687
BpuMI CCSGG 1 cut(s) 230
BsaAI YACGTR 1 cut(s) 1129
BsaBI GATNNNNATC 1 cut(s) 60
BsaI GGTCTC 2 cut(s) 337, 1242
BsaJI CCNNGG 1 cut(s) 955
BsaWI WCCGGW 1 cut(s) 768
Bsc4I CCNNNNNNNGG 4 cut(s) 129, 235, 981, 1212
Bse1I ACTGG 2 cut(s) 811, 975
Bse8I GATNNNNATC 1 cut(s) 60
BseAI TCCGGA 1 cut(s) 768
BseBI CCWGG 1 cut(s) 196
BseDI CCNNGG 1 cut(s) 955
BseGI GGATG 5 cut(s) 516, 558, 777, 1238, 1242
BseJI GATNNNNATC 1 cut(s) 60
BseLI CCNNNNNNNGG 4 cut(s) 129, 235, 981, 1212
BseMII CTCAG 1 cut(s) 310
BseNI ACTGG 2 cut(s) 811, 975
BseRI GAGGAG 2 cut(s) 734, 1244
BseXI GCAGC 2 cut(s) 468, 941
BseYI CCCAGC 1 cut(s) 1143
BsgI GTGCAG 1 cut(s) 806
BshFI GGCC 2 cut(s) 408, 1058
BsiHKAI GWGCWC 2 cut(s) 297, 834
BsiHKCI CYCGRG 1 cut(s) 827
BsiSI CCGG 2 cut(s) 230, 769
BslI CCNNNNNNNGG 4 cut(s) 129, 235, 981, 1212
BsmAI GTCTC 4 cut(s) 337, 889, 1171, 1242
BsmI GAATGC 2 cut(s) 101, 372
BsnI GGCC 2 cut(s) 408, 1058
Bso31I GGTCTC 2 cut(s) 337, 1242
BsoBI CYCGRG 1 cut(s) 827
Bsp119I TTCGAA 2 cut(s) 424, 687
Bsp1286I GDGCHC 3 cut(s) 297, 834, 955
Bsp13I TCCGGA 1 cut(s) 768
Bsp143I GATC 3 cut(s) 25, 394, 1263
BspACI CCGC 8 cut(s) 5, 29, 317, 326, 329, 520, 736, 1059
BspANI GGCC 2 cut(s) 408, 1058
BspCNI CTCAG 1 cut(s) 309
BspEI TCCGGA 1 cut(s) 768
BspLI GGNNCC 4 cut(s) 9, 27, 383, 767
BspPI GGATC 2 cut(s) 20, 33
BspQI GCTCTTC 1 cut(s) 689
BspT104I TTCGAA 2 cut(s) 424, 687
BspTNI GGTCTC 2 cut(s) 337, 1242
BsrI ACTGG 2 cut(s) 811, 975
BssECI CCNNGG 1 cut(s) 955
BssMI GATC 3 cut(s) 25, 394, 1263
BssT1I CCWWGG 1 cut(s) 955
Bst2UI CCWGG 1 cut(s) 196
Bst4CI ACNGT 8 cut(s) 74, 257, 356, 845, 859, 869, 970, 1040
Bst6I CTCTTC 1 cut(s) 689
BstBAI YACGTR 1 cut(s) 1129
BstBI TTCGAA 2 cut(s) 424, 687
BstC8I GCNNGC 7 cut(s) 315, 464, 622, 734, 927, 987, 1149
BstDEI CTNAG 2 cut(s) 296, 1085
BstF5I GGATG 5 cut(s) 516, 558, 777, 1238, 1242
BstH2I RGCGCY 1 cut(s) 482
BstHHI GCGC 2 cut(s) 190, 481
BstKTI GATC 3 cut(s) 28, 397, 1266
BstMAI GTCTC 4 cut(s) 337, 889, 1171, 1242
BstMBI GATC 3 cut(s) 25, 394, 1263
BstMWI GCNNNNNNNGC 4 cut(s) 314, 323, 369, 405
BstNI CCWGG 1 cut(s) 196
BstNSI RCATGY 3 cut(s) 929, 989, 1002
BstSCI CCNGG 2 cut(s) 194, 228
BstSFI CTRYAG 1 cut(s) 400
BstV1I GCAGC 2 cut(s) 468, 941
BstV2I GAAGAC 1 cut(s) 350
BstX2I RGATCY 1 cut(s) 25
BstYI RGATCY 1 cut(s) 25
BsuI GTATCC 1 cut(s) 97
BsuRI GGCC 2 cut(s) 408, 1058
BtgZI GCGATG 1 cut(s) 294
BtsCI GGATG 5 cut(s) 516, 558, 777, 1238, 1242
BtsI GCAGTG 1 cut(s) 1076
BtsIMutI CAGTG 5 cut(s) 352, 850, 855, 874, 1076
Cac8I GCNNGC 7 cut(s) 315, 464, 622, 734, 927, 987, 1149
CfoI GCGC 2 cut(s) 190, 481
Cfr13I GGNCC 2 cut(s) 7, 115
Csp6I GTAC 1 cut(s) 603
CviAII CATG 7 cut(s) 486, 523, 546, 588, 926, 986, 999
CviQI GTAC 1 cut(s) 603
DdeI CTNAG 2 cut(s) 296, 1085
DpnI GATC 3 cut(s) 27, 396, 1265
DpnII GATC 3 cut(s) 25, 394, 1263
EaeI YGGCCR 1 cut(s) 406
Eam1104I CTCTTC 1 cut(s) 689
EarI CTCTTC 1 cut(s) 689
EciI GGCGGA 2 cut(s) 18, 20
Ecl136II GAGCTC 2 cut(s) 295, 832
Eco130I CCWWGG 1 cut(s) 955
Eco24I GRGCYC 3 cut(s) 297, 834, 955
Eco31I GGTCTC 2 cut(s) 337, 1242
Eco32I GATATC 1 cut(s) 528
Eco47I GGWCC 2 cut(s) 7, 115
Eco53kI GAGCTC 2 cut(s) 295, 832
Eco72I CACGTG 1 cut(s) 1129
Eco88I CYCGRG 1 cut(s) 827
EcoICRI GAGCTC 2 cut(s) 295, 832
EcoRII CCWGG 1 cut(s) 194
EcoRV GATATC 1 cut(s) 528
EcoT14I CCWWGG 1 cut(s) 955
EcoT38I GRGCYC 3 cut(s) 297, 834, 955
ErhI CCWWGG 1 cut(s) 955
FaeI CATG 7 cut(s) 489, 526, 549, 591, 929, 989, 1002
FatI CATG 7 cut(s) 485, 522, 545, 587, 925, 985, 998
FauNDI CATATG 1 cut(s) 1158
Fnu4HI GCNGC 4 cut(s) 327, 482, 930, 1059
FokI GGATG 5 cut(s) 503, 565, 784, 1245, 1249
FriOI GRGCYC 3 cut(s) 297, 834, 955
Fsp4HI GCNGC 4 cut(s) 327, 482, 930, 1059
FspBI CTAG 2 cut(s) 134, 242
GlaI GCGC 2 cut(s) 189, 480
GluI GCNGC 4 cut(s) 327, 482, 930, 1059
GsaI CCCAGC 1 cut(s) 1147
HaeII RGCGCY 1 cut(s) 482
HaeIII GGCC 2 cut(s) 408, 1058
HapII CCGG 2 cut(s) 230, 769
HhaI GCGC 2 cut(s) 190, 481
Hin1II CATG 7 cut(s) 489, 526, 549, 591, 929, 989, 1002
Hin6I GCGC 2 cut(s) 188, 479
HinP1I GCGC 2 cut(s) 188, 479
HindIII AAGCTT 1 cut(s) 1182
HinfI GANTC 4 cut(s) 56, 695, 820, 1109
HpaII CCGG 2 cut(s) 230, 769
HphI GGTGA 4 cut(s) 68, 793, 1057, 1249
Hpy166II GTNNAC 4 cut(s) 1048, 1076, 1126, 1326
Hpy188I TCNGA 8 cut(s) 37, 262, 378, 1036, 1096, 1232, 1287, 1308
Hpy188III TCNNGA 2 cut(s) 53, 769
Hpy8I GTNNAC 4 cut(s) 1048, 1076, 1126, 1326
HpyAV CCTTC 5 cut(s) 42, 56, 83, 672, 788
HpyCH4III ACNGT 8 cut(s) 74, 257, 356, 845, 859, 869, 970, 1040
HpyCH4IV ACGT 2 cut(s) 140, 1128
HpyF10VI GCNNNNNNNGC 4 cut(s) 314, 323, 369, 405
HpyF3I CTNAG 2 cut(s) 296, 1085
HpySE526I ACGT 2 cut(s) 140, 1128
Hsp92II CATG 7 cut(s) 489, 526, 549, 591, 929, 989, 1002
HspAI GCGC 2 cut(s) 188, 479
Kpn2I TCCGGA 1 cut(s) 768
Kzo9I GATC 3 cut(s) 25, 394, 1263
LguI GCTCTTC 1 cut(s) 689
LmnI GCTCC 1 cut(s) 721
Lsp1109I GCAGC 2 cut(s) 468, 941
LweI GCATC 3 cut(s) 317, 354, 1090
MaeI CTAG 2 cut(s) 134, 242
MaeII ACGT 2 cut(s) 140, 1128
MaeIII GTNAC 4 cut(s) 74, 799, 817, 1088
MalI GATC 3 cut(s) 27, 396, 1265
MboI GATC 3 cut(s) 25, 394, 1263
MboII GAAGA 5 cut(s) 41, 162, 203, 350, 676
MfeI CAATTG 2 cut(s) 110, 891
MflI RGATCY 1 cut(s) 25
MhlI GDGCHC 3 cut(s) 297, 834, 955
MluCI AATT 5 cut(s) 110, 891, 939, 1012, 1138
MlyI GAGTC 2 cut(s) 814, 1118
MmeI TCCRAC 1 cut(s) 151
MroI TCCGGA 1 cut(s) 768
MroXI GAANNNNTTC 1 cut(s) 141
MseI TTAA 3 cut(s) 282, 447, 992
MslI CAYNNNNRTG 2 cut(s) 521, 666
MspA1I CMGCKG 1 cut(s) 1147
MspI CCGG 2 cut(s) 230, 769
MspR9I CCNGG 2 cut(s) 196, 230
MunI CAATTG 2 cut(s) 110, 891
Mva1269I GAATGC 2 cut(s) 101, 372
MvaI CCWGG 1 cut(s) 196
MwoI GCNNNNNNNGC 4 cut(s) 314, 323, 369, 405
NciI CCSGG 1 cut(s) 230
NdeI CATATG 1 cut(s) 1158
NdeII GATC 3 cut(s) 25, 394, 1263
NlaIII CATG 7 cut(s) 489, 526, 549, 591, 929, 989, 1002
NlaIV GGNNCC 4 cut(s) 9, 27, 383, 767
NmuCI GTSAC 3 cut(s) 74, 799, 817
NspI RCATGY 3 cut(s) 929, 989, 1002
NspV TTCGAA 2 cut(s) 424, 687
PaeI GCATGC 2 cut(s) 929, 989
PaeR7I CTCGAG 1 cut(s) 827
PciSI GCTCTTC 1 cut(s) 689
PctI GAATGC 2 cut(s) 101, 372
PdmI GAANNNNTTC 1 cut(s) 141
PfeI GAWTC 2 cut(s) 56, 695
PflMI CCANNNNNTGG 1 cut(s) 981
PfoI TCCNGGA 1 cut(s) 194
PkrI GCNGC 4 cut(s) 328, 483, 931, 1060
PleI GAGTC 2 cut(s) 814, 1117
PmaCI CACGTG 1 cut(s) 1129
PmlI CACGTG 1 cut(s) 1129
PpsI GAGTC 2 cut(s) 814, 1117
Ppu21I YACGTR 1 cut(s) 1129
PsiI TTATAA 1 cut(s) 794
Psp124BI GAGCTC 2 cut(s) 297, 834
Psp1406I AACGTT 1 cut(s) 140
Psp6I CCWGG 1 cut(s) 194
PspCI CACGTG 1 cut(s) 1129
PspFI CCCAGC 1 cut(s) 1143
PspGI CCWGG 1 cut(s) 194
PspN4I GGNNCC 4 cut(s) 9, 27, 383, 767
PspPI GGNCC 2 cut(s) 7, 115
PspXI VCTCGAGB 1 cut(s) 827
PsrI GAACNNNNNNTAC 2 cut(s) 564, 596
PsuI RGATCY 1 cut(s) 25
PvuII CAGCTG 1 cut(s) 1147
RsaI GTAC 1 cut(s) 604
RsaNI GTAC 1 cut(s) 603
RseI CAYNNNNRTG 2 cut(s) 521, 666
SacI GAGCTC 2 cut(s) 297, 834
SapI GCTCTTC 1 cut(s) 689
SaqAI TTAA 3 cut(s) 282, 447, 992
SatI GCNGC 4 cut(s) 327, 482, 930, 1059
Sau3AI GATC 3 cut(s) 25, 394, 1263
Sau96I GGNCC 2 cut(s) 7, 115
SchI GAGTC 2 cut(s) 814, 1118
ScrFI CCNGG 2 cut(s) 196, 230
SduI GDGCHC 3 cut(s) 297, 834, 955
SfaNI GCATC 3 cut(s) 317, 354, 1090
SfcI CTRYAG 1 cut(s) 400
Sfr274I CTCGAG 1 cut(s) 827
SfuI TTCGAA 2 cut(s) 424, 687
SinI GGWCC 2 cut(s) 7, 115
SlaI CTCGAG 1 cut(s) 827
SmiMI CAYNNNNRTG 2 cut(s) 521, 666
SmlI CTYRAG 1 cut(s) 827
SmoI CTYRAG 1 cut(s) 827
SphI GCATGC 2 cut(s) 929, 989
Sse9I AATT 5 cut(s) 110, 891, 939, 1012, 1138
SsiI CCGC 8 cut(s) 5, 29, 317, 326, 329, 520, 736, 1059
SspMI CTAG 2 cut(s) 134, 242
SstI GAGCTC 2 cut(s) 297, 834
StyD4I CCNGG 2 cut(s) 194, 228
StyI CCWWGG 1 cut(s) 955
TaaI ACNGT 8 cut(s) 74, 257, 356, 845, 859, 869, 970, 1040
TaiI ACGT 2 cut(s) 143, 1131
TaqI TCGA 9 cut(s) 54, 179, 291, 424, 666, 687, 828, 1242, 1275
TaqII GACCGA 1 cut(s) 152
TasI AATT 5 cut(s) 110, 891, 939, 1012, 1138
TatI WGTACW 1 cut(s) 602
TauI GCSGC 2 cut(s) 329, 1061
TfiI GAWTC 2 cut(s) 56, 695
Tru1I TTAA 3 cut(s) 282, 447, 992
Tru9I TTAA 3 cut(s) 282, 447, 992
TscAI CASTG 5 cut(s) 359, 850, 862, 874, 1083
TseFI GTSAC 3 cut(s) 74, 799, 817
TseI GCWGC 2 cut(s) 481, 929
Tsp45I GTSAC 3 cut(s) 74, 799, 817
TspDTI ATGAA 3 cut(s) 1005, 1155, 1261
TspRI CASTG 5 cut(s) 359, 850, 862, 874, 1083
Van91I CCANNNNNTGG 1 cut(s) 981
VpaK11BI GGWCC 2 cut(s) 7, 115
XapI RAATTY 2 cut(s) 939, 1138
XceI RCATGY 3 cut(s) 929, 989, 1002
XhoI CTCGAG 1 cut(s) 827
XmnI GAANNNNTTC 1 cut(s) 141
XspI CTAG 2 cut(s) 134, 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.