pycom13g09910

Protein of unknown function (DUF3537)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0007442
Physical Location & Seq
Reverse (-)
6599636 .. 6600915
1280 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g09910.1

Sequence Viewer

Length: 675 bp
ATGGCGGACCCTCATATAAAAACAGATGAGCCACCTTCCCAGCTTACCCTTCTTCTCGATTCAAATCACAACAACCGCCACCAAGCAGCAGAAGGAGAATACAAAGACACCCAATTGGACCAAACCCTCCAACGGCTAGAAACGTTTCTCACTCTCTTGGGCTTCGATCAATCTTCTTGGTTGAGCCTTGCGCTGTCCTGGACTGCTCTGGTTTTTCTCGGTGTGGTGCTTCCGGTGGTCGTGCTCGAGATGTTCAACTGCTCGGACTGCGAGAAGTATCAGGTAAAGGGGTTCGAGCTCGTTATCGTGGGCTCGCAGGCGGTTCTGGCGGCGGTCTCTTTGCTCTGCATCTCCCATTGTCTTCGCAAGTATGGCATTCGGAGGTTCCTCTTTGTCGATCGCTATAGTGGCCGTATGGTTCGGTTTCGAAACGATTACATCAGGCAGATTAAGGGTTCTTTACGCCTGCTCATTTGGTGGGCGCTGCCGTGTTTTCTTTTGAAGACTTTGCGTGAGGTCATCCGGATGATATATGTCCATCAGTCATGGTGGCTGTCATTTGCTGTATTACTAGCTTTGGTTCTATCATGGGCTTATGTGAGTACAATCACTCTAACAGCCAGCATTTTATTTCACTTGATCGATCTGCAACTTGCAAGTTATCCACTTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3537

Protein Analysis

225

Amino Acids

25.85

Weight (kDa)

8.61

Isoelectric Point (pI)

32.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3537 PF12056 42 - 214 3.3e-54 Protein of unknown function (DUF3537)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 522
AciI CCGC 5 cut(s) 5, 76, 320, 329, 332
AclI AACGTT 1 cut(s) 143
AcoI YGGCCR 1 cut(s) 409
AfaI GTAC 1 cut(s) 604
AfiI CCNNNNNNNGG 1 cut(s) 132
AgsI TTSAA 3 cut(s) 63, 256, 502
AjnI CCWGG 1 cut(s) 197
AluBI AGCT 3 cut(s) 43, 298, 575
AluI AGCT 3 cut(s) 43, 298, 575
Alw21I GWGCWC 2 cut(s) 246, 300
Alw26I GTCTC 1 cut(s) 340
Ama87I CYCGRG 1 cut(s) 245
Aor13HI TCCGGA 1 cut(s) 522
AoxI GGCC 1 cut(s) 409
ApeKI GCWGC 2 cut(s) 86, 484
Asp700I GAANNNNTTC 1 cut(s) 144
AspLEI GCGC 2 cut(s) 193, 484
AspS9I GGNCC 2 cut(s) 7, 118
AsuII TTCGAA 1 cut(s) 427
AvaI CYCGRG 1 cut(s) 245
AvaII GGWCC 2 cut(s) 7, 118
BanII GRGCYC 2 cut(s) 300, 314
BbsI GAAGAC 2 cut(s) 353, 509
Bbv12I GWGCWC 2 cut(s) 246, 300
BbvI GCAGC 2 cut(s) 98, 471
BccI CCATC 1 cut(s) 546
BceAI ACGGC 3 cut(s) 149, 396, 472
BciT130I CCWGG 1 cut(s) 199
BcoDI GTCTC 1 cut(s) 340
BfaI CTAG 2 cut(s) 137, 572
BfmI CTRYAG 1 cut(s) 403
BfoI RGCGCY 1 cut(s) 485
BisI GCNGC 3 cut(s) 87, 330, 485
BlsI GCNGC 3 cut(s) 88, 331, 486
Bme1390I CCNGG 1 cut(s) 199
Bme18I GGWCC 2 cut(s) 7, 118
BmeT110I CYCGRG 1 cut(s) 245
BmgT120I GGNCC 2 cut(s) 7, 118
BmiI GGNNCC 2 cut(s) 9, 386
BmrFI CCNGG 1 cut(s) 199
BmsI GCATC 1 cut(s) 357
BpiI GAAGAC 2 cut(s) 353, 509
Bpu14I TTCGAA 1 cut(s) 427
Bsa29I ATCGAT 1 cut(s) 642
BsaBI GATNNNNATC 1 cut(s) 63
BsaI GGTCTC 1 cut(s) 340
BsaWI WCCGGW 2 cut(s) 232, 522
Bsc4I CCNNNNNNNGG 1 cut(s) 132
Bse8I GATNNNNATC 1 cut(s) 63
BseAI TCCGGA 1 cut(s) 522
BseBI CCWGG 1 cut(s) 199
BseCI ATCGAT 1 cut(s) 642
BseGI GGATG 2 cut(s) 519, 531
BseJI GATNNNNATC 1 cut(s) 63
BseLI CCNNNNNNNGG 1 cut(s) 132
BseXI GCAGC 2 cut(s) 98, 471
BseYI CCCAGC 1 cut(s) 39
Bsh1285I CGRYCG 1 cut(s) 400
BshFI GGCC 1 cut(s) 411
BshVI ATCGAT 1 cut(s) 642
BsiEI CGRYCG 1 cut(s) 400
BsiHKAI GWGCWC 2 cut(s) 246, 300
BsiHKCI CYCGRG 1 cut(s) 245
BsiSI CCGG 2 cut(s) 233, 523
BslI CCNNNNNNNGG 1 cut(s) 132
BsmAI GTCTC 1 cut(s) 340
BsmI GAATGC 1 cut(s) 375
BsnI GGCC 1 cut(s) 411
Bso31I GGTCTC 1 cut(s) 340
BsoBI CYCGRG 1 cut(s) 245
Bsp119I TTCGAA 1 cut(s) 427
Bsp1286I GDGCHC 3 cut(s) 246, 300, 314
Bsp13I TCCGGA 1 cut(s) 522
Bsp143I GATC 4 cut(s) 166, 397, 639, 643
BspACI CCGC 5 cut(s) 5, 76, 320, 329, 332
BspANI GGCC 1 cut(s) 411
BspDI ATCGAT 1 cut(s) 642
BspEI TCCGGA 1 cut(s) 522
BspLI GGNNCC 2 cut(s) 9, 386
BspT104I TTCGAA 1 cut(s) 427
BspTNI GGTCTC 1 cut(s) 340
BssMI GATC 4 cut(s) 166, 397, 639, 643
Bst2UI CCWGG 1 cut(s) 199
BstBI TTCGAA 1 cut(s) 427
BstC8I GCNNGC 4 cut(s) 314, 318, 467, 622
BstF5I GGATG 2 cut(s) 519, 531
BstH2I RGCGCY 1 cut(s) 485
BstHHI GCGC 2 cut(s) 193, 484
BstKTI GATC 4 cut(s) 169, 400, 642, 646
BstMAI GTCTC 1 cut(s) 340
BstMBI GATC 4 cut(s) 166, 397, 639, 643
BstMCI CGRYCG 1 cut(s) 400
BstMWI GCNNNNNNNGC 4 cut(s) 267, 326, 372, 408
BstNI CCWGG 1 cut(s) 199
BstSCI CCNGG 1 cut(s) 197
BstSFI CTRYAG 1 cut(s) 403
BstV1I GCAGC 2 cut(s) 98, 471
BstV2I GAAGAC 2 cut(s) 353, 509
Bsu15I ATCGAT 1 cut(s) 642
BsuRI GGCC 1 cut(s) 411
BsuTUI ATCGAT 1 cut(s) 642
BtsCI GGATG 2 cut(s) 519, 531
Cac8I GCNNGC 4 cut(s) 314, 318, 467, 622
CfoI GCGC 2 cut(s) 193, 484
Cfr13I GGNCC 2 cut(s) 7, 118
ClaI ATCGAT 1 cut(s) 642
Csp6I GTAC 1 cut(s) 603
CviAII CATG 2 cut(s) 546, 588
CviQI GTAC 1 cut(s) 603
DpnI GATC 4 cut(s) 168, 399, 641, 645
DpnII GATC 4 cut(s) 166, 397, 639, 643
EaeI YGGCCR 1 cut(s) 409
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 298
Eco24I GRGCYC 2 cut(s) 300, 314
Eco31I GGTCTC 1 cut(s) 340
Eco47I GGWCC 2 cut(s) 7, 118
Eco53kI GAGCTC 1 cut(s) 298
Eco88I CYCGRG 1 cut(s) 245
EcoICRI GAGCTC 1 cut(s) 298
EcoRII CCWGG 1 cut(s) 197
EcoT38I GRGCYC 2 cut(s) 300, 314
FaeI CATG 2 cut(s) 549, 591
FatI CATG 2 cut(s) 545, 587
Fnu4HI GCNGC 3 cut(s) 87, 330, 485
FokI GGATG 2 cut(s) 506, 538
FriOI GRGCYC 2 cut(s) 300, 314
Fsp4HI GCNGC 3 cut(s) 87, 330, 485
FspBI CTAG 2 cut(s) 137, 572
GlaI GCGC 2 cut(s) 192, 483
GluI GCNGC 3 cut(s) 87, 330, 485
GsaI CCCAGC 1 cut(s) 43
HaeII RGCGCY 1 cut(s) 485
HaeIII GGCC 1 cut(s) 411
HapII CCGG 2 cut(s) 233, 523
HhaI GCGC 2 cut(s) 193, 484
Hin1II CATG 2 cut(s) 549, 591
Hin6I GCGC 2 cut(s) 191, 482
HinP1I GCGC 2 cut(s) 191, 482
HinfI GANTC 1 cut(s) 59
HpaII CCGG 2 cut(s) 233, 523
Hpy188I TCNGA 2 cut(s) 265, 381
Hpy188III TCNNGA 3 cut(s) 56, 247, 523
HpyAV CCTTC 3 cut(s) 45, 59, 86
HpyCH4IV ACGT 1 cut(s) 143
HpyCH4V TGCA 3 cut(s) 348, 649, 656
HpyF10VI GCNNNNNNNGC 4 cut(s) 267, 326, 372, 408
HpySE526I ACGT 1 cut(s) 143
Hsp92II CATG 2 cut(s) 549, 591
HspAI GCGC 2 cut(s) 191, 482
Kpn2I TCCGGA 1 cut(s) 522
Kzo9I GATC 4 cut(s) 166, 397, 639, 643
Lsp1109I GCAGC 2 cut(s) 98, 471
LweI GCATC 1 cut(s) 357
MaeI CTAG 2 cut(s) 137, 572
MaeII ACGT 1 cut(s) 143
MalI GATC 4 cut(s) 168, 399, 641, 645
MboI GATC 4 cut(s) 166, 397, 639, 643
MboII GAAGA 4 cut(s) 44, 165, 353, 514
MfeI CAATTG 1 cut(s) 113
MhlI GDGCHC 3 cut(s) 246, 300, 314
MluCI AATT 1 cut(s) 113
MmeI TCCRAC 1 cut(s) 154
MnlI CCTC 5 cut(s) 21, 137, 375, 398, 508
MroI TCCGGA 1 cut(s) 522
MroXI GAANNNNTTC 1 cut(s) 144
MseI TTAA 1 cut(s) 450
MslI CAYNNNNRTG 2 cut(s) 524, 670
MspI CCGG 2 cut(s) 233, 523
MspR9I CCNGG 1 cut(s) 199
MunI CAATTG 1 cut(s) 113
Mva1269I GAATGC 1 cut(s) 375
MvaI CCWGG 1 cut(s) 199
MwoI GCNNNNNNNGC 4 cut(s) 267, 326, 372, 408
NdeII GATC 4 cut(s) 166, 397, 639, 643
NlaIII CATG 2 cut(s) 549, 591
NlaIV GGNNCC 2 cut(s) 9, 386
NspV TTCGAA 1 cut(s) 427
PaeR7I CTCGAG 1 cut(s) 245
PctI GAATGC 1 cut(s) 375
PdmI GAANNNNTTC 1 cut(s) 144
PfeI GAWTC 1 cut(s) 59
PfoI TCCNGGA 1 cut(s) 197
PkrI GCNGC 3 cut(s) 88, 331, 486
Ple19I CGATCG 1 cut(s) 400
Psp124BI GAGCTC 1 cut(s) 300
Psp1406I AACGTT 1 cut(s) 143
Psp6I CCWGG 1 cut(s) 197
PspFI CCCAGC 1 cut(s) 39
PspGI CCWGG 1 cut(s) 197
PspN4I GGNNCC 2 cut(s) 9, 386
PspPI GGNCC 2 cut(s) 7, 118
PvuI CGATCG 1 cut(s) 400
RsaI GTAC 1 cut(s) 604
RsaNI GTAC 1 cut(s) 603
RseI CAYNNNNRTG 2 cut(s) 524, 670
SacI GAGCTC 1 cut(s) 300
SaqAI TTAA 1 cut(s) 450
SatI GCNGC 3 cut(s) 87, 330, 485
Sau3AI GATC 4 cut(s) 166, 397, 639, 643
Sau96I GGNCC 2 cut(s) 7, 118
ScrFI CCNGG 1 cut(s) 199
SduI GDGCHC 3 cut(s) 246, 300, 314
SetI ASST 8 cut(s) 37, 45, 146, 285, 300, 386, 519, 577
SfaNI GCATC 1 cut(s) 357
SfcI CTRYAG 1 cut(s) 403
Sfr274I CTCGAG 1 cut(s) 245
SfuI TTCGAA 1 cut(s) 427
SinI GGWCC 2 cut(s) 7, 118
SlaI CTCGAG 1 cut(s) 245
SmiMI CAYNNNNRTG 2 cut(s) 524, 670
SmlI CTYRAG 1 cut(s) 245
SmoI CTYRAG 1 cut(s) 245
Sse9I AATT 1 cut(s) 113
SsiI CCGC 5 cut(s) 5, 76, 320, 329, 332
SspMI CTAG 2 cut(s) 137, 572
SstI GAGCTC 1 cut(s) 300
StyD4I CCNGG 1 cut(s) 197
TaiI ACGT 1 cut(s) 146
TaqI TCGA 8 cut(s) 57, 165, 246, 294, 396, 427, 642, 670
TasI AATT 1 cut(s) 113
TatI WGTACW 1 cut(s) 602
TauI GCSGC 1 cut(s) 332
TfiI GAWTC 1 cut(s) 59
Tru1I TTAA 1 cut(s) 450
Tru9I TTAA 1 cut(s) 450
TseI GCWGC 2 cut(s) 86, 484
VpaK11BI GGWCC 2 cut(s) 7, 118
XhoI CTCGAG 1 cut(s) 245
XmnI GAANNNNTTC 1 cut(s) 144
XspI CTAG 2 cut(s) 137, 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.