FvH4_4g24190

Protein of unknown function (DUF3537)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0007442
Physical Location & Seq
Forward (+)
26449350 .. 26452882
3533 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g24190.t1

Sequence Viewer

Length: 1353 bp
ATGGCTGACCCTCATACTAAAACAGAGGACCTCCCACCACTCCACCCCAACACCACCACCACCCCTCTTCTCGATTCCAACCATACCCACCACCCAGAAGAACAAGGCCTCGAAATCAAAGATACCCAGTTGGATCAAACCCTTCAAAAGCTTGAAGCTTTCCTCACTCTGTTGGGTTTCAACCAAACTTGTATTTTCAGCTGTATACTATCTTGGTTTGCTTTTGTGATCATCGGAGTATTGCTTCCGGTGCTTGTTCTTGTGATTTTCAACTGTTCTGATTGCGATAAGTTCCAGATTGAGGGGTTTGAGCTTGTTATTGTGGGCTCTCAGGCTTGTTTGGCTGCTGTGTCTTTGCTTTGTTTATCTCACAATCTTCGCAAGTATGGCATTCGGAGATTCTTGTTTGTTGATCGGTATACTGGCCATATGGTTCGGTTTCGAAACGATTACATAAATCAGATTAAGGGTTCTTTTCGGTTGCTACTTTGGTGGTCACTGCCATGTTTGCTGTTGAAGACTATACGTGAAGTGGTCCGCATGCTATATGTCCATCATGATTCATGGTGGCTCTCAGTTTCTATTTTATTAGCTTTGGTTCTATCATGGACATACGCGAGTACAATCACCCTCACAGCCAGCATTTTGTTTCACTTGGTCTGCAATTTGCAAGTAATCCACTTCGAGGATTATGGGAGGCTCTTGGAAAGGGAATCTGATGTTTTGGTATTTATGGAGGAGCACATTCGGCTTCGGTATCACCTCTCTAAGATAAGCCACAGGTTTCGGATCTTCCTACTTCTGCAGTTTTTGGTTGTCACATCAAGCCAGTTTGTGACTCTATTTGAGGTCACTGGATATAGTGGAAAGATCACATTCATTAATGGTGGTGATTTTGCTGTCTCCACAATTGTTCAGGTAGTCGGGATTATTATTTCTCTACACGCAGCAACCAGAATTTCTCACAGAGCCCAGGCCTTAGCATCACTTGCCAGTAGATGGCACGCACTAGTAACATCCAGTTCTAATGATTCATCTCACAACAGAAATTCAAACAGTACGGGGAACCTTGAGGCTGCCAACACAATTAACTCGCTGCATCTAAGTTACTCTGAGAGTGATTTGGAGTCGTGTGATTATTATGTTTCAATGCCTACAAATACACAGCTGGCTTCATATATGTCCTCATATCACAGGAGGCAAGCTTTTGTAATGTATTTGCAAACTAATGCTGGAGGGATCACAATATTTGGGTGGACAGTTGATAGAGCCCTCCTCAACACAATCTTCTTCATCGAACTATCTCTGATTACCTTTGTGCTTGGTAAAACAATAGTTTTCTCATCGACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3537

Protein Analysis

451

Amino Acids

51.13

Weight (kDa)

6.69

Isoelectric Point (pI)

36.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3537 PF12056 47 - 432 1.2e-147 Protein of unknown function (DUF3537)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 999
AccI GTMKAC 2 cut(s) 205, 419
AccII CGCG 1 cut(s) 617
AciI CCGC 1 cut(s) 538
AclWI GGATC 3 cut(s) 141, 797, 1247
AcoI YGGCCR 1 cut(s) 424
AcsI RAATTY 2 cut(s) 957, 1048
AfaI GTAC 2 cut(s) 622, 1060
AfiI CCNNNNNNNGG 3 cut(s) 301, 685, 999
AgsI TTSAA 7 cut(s) 146, 155, 181, 271, 517, 1053, 1149
AhlI ACTAGT 1 cut(s) 1009
AjnI CCWGG 1 cut(s) 972
AluBI AGCT 7 cut(s) 151, 158, 201, 313, 593, 1168, 1205
AluI AGCT 7 cut(s) 151, 158, 201, 313, 593, 1168, 1205
Alw21I GWGCWC 1 cut(s) 744
Alw26I GTCTC 1 cut(s) 907
AlwI GGATC 3 cut(s) 141, 797, 1247
AoxI GGCC 3 cut(s) 106, 424, 975
ApeKI GCWGC 4 cut(s) 344, 947, 1076, 1096
ApoI RAATTY 2 cut(s) 957, 1048
AseI ATTAAT 1 cut(s) 882
AspS9I GGNCC 2 cut(s) 28, 535
AsuHPI GGTGA 3 cut(s) 619, 752, 902
AsuII TTCGAA 1 cut(s) 442
AvaII GGWCC 2 cut(s) 28, 535
BalI TGGCCA 1 cut(s) 426
BanII GRGCYC 3 cut(s) 329, 973, 1273
BbsI GAAGAC 1 cut(s) 524
Bbv12I GWGCWC 1 cut(s) 744
BbvI GCAGC 4 cut(s) 331, 959, 1063, 1083
BccI CCATC 2 cut(s) 561, 993
BciT130I CCWGG 1 cut(s) 974
BclI TGATCA 1 cut(s) 228
BcoDI GTCTC 1 cut(s) 907
BcuI ACTAGT 1 cut(s) 1009
BfaI CTAG 1 cut(s) 1010
BfmI CTRYAG 1 cut(s) 803
BisI GCNGC 4 cut(s) 345, 948, 1077, 1097
BlsI GCNGC 4 cut(s) 346, 949, 1078, 1098
Bme1390I CCNGG 1 cut(s) 974
Bme18I GGWCC 2 cut(s) 28, 535
BmgT120I GGNCC 2 cut(s) 28, 535
BmiI GGNNCC 1 cut(s) 1067
BmrFI CCNGG 1 cut(s) 974
BmrI ACTGGG 1 cut(s) 121
BmsI GCATC 2 cut(s) 992, 1108
BmuI ACTGGG 1 cut(s) 121
BpiI GAAGAC 1 cut(s) 524
BplI GAGNNNNNCTC 2 cut(s) 1260, 1292
BpmI CTGGAG 1 cut(s) 1254
Bpu10I CCTNAGC 1 cut(s) 979
Bpu14I TTCGAA 1 cut(s) 442
BpuEI CTTGAG 1 cut(s) 1091
BsaAI YACGTR 1 cut(s) 527
BsaJI CCNNGG 1 cut(s) 972
BsaWI WCCGGW 1 cut(s) 247
Bsc4I CCNNNNNNNGG 3 cut(s) 301, 685, 999
Bse1I ACTGG 6 cut(s) 127, 427, 829, 859, 993, 1020
BseBI CCWGG 1 cut(s) 974
BseDI CCNNGG 1 cut(s) 972
BseGI GGATG 1 cut(s) 1016
BseLI CCNNNNNNNGG 3 cut(s) 301, 685, 999
BseMII CTCAG 3 cut(s) 344, 588, 1104
BseNI ACTGG 6 cut(s) 127, 427, 829, 859, 993, 1020
BseRI GAGGAG 2 cut(s) 752, 1265
BseXI GCAGC 4 cut(s) 331, 959, 1063, 1083
Bsh1236I CGCG 1 cut(s) 617
BshFI GGCC 3 cut(s) 108, 426, 977
BsiHKAI GWGCWC 1 cut(s) 744
BsiSI CCGG 1 cut(s) 248
BslI CCNNNNNNNGG 3 cut(s) 301, 685, 999
BsmAI GTCTC 1 cut(s) 907
BsmI GAATGC 1 cut(s) 390
BsnI GGCC 3 cut(s) 108, 426, 977
Bsp119I TTCGAA 1 cut(s) 442
Bsp1286I GDGCHC 4 cut(s) 329, 744, 973, 1273
Bsp143I GATC 6 cut(s) 133, 228, 412, 789, 870, 1239
BspACI CCGC 1 cut(s) 538
BspANI GGCC 3 cut(s) 108, 426, 977
BspCNI CTCAG 3 cut(s) 343, 587, 1105
BspFNI CGCG 1 cut(s) 617
BspHI TCATGA 1 cut(s) 556
BspLI GGNNCC 1 cut(s) 1067
BspMAI CTGCAG 1 cut(s) 807
BspPI GGATC 3 cut(s) 141, 797, 1247
BspT104I TTCGAA 1 cut(s) 442
BsrI ACTGG 6 cut(s) 127, 427, 829, 859, 993, 1020
BssECI CCNNGG 1 cut(s) 972
BssMI GATC 6 cut(s) 133, 228, 412, 789, 870, 1239
BssNAI GTATAC 2 cut(s) 206, 420
Bst1107I GTATAC 2 cut(s) 206, 420
Bst2UI CCWGG 1 cut(s) 974
Bst4CI ACNGT 3 cut(s) 275, 1058, 1261
Bst6I CTCTTC 1 cut(s) 72
BstAPI GCANNNNNTGC 1 cut(s) 989
BstBAI YACGTR 1 cut(s) 527
BstBI TTCGAA 1 cut(s) 442
BstC8I GCNNGC 5 cut(s) 542, 640, 1005, 1170, 1203
BstDEI CTNAG 6 cut(s) 330, 574, 768, 979, 1103, 1113
BstF5I GGATG 1 cut(s) 1016
BstFNI CGCG 1 cut(s) 617
BstKTI GATC 6 cut(s) 136, 231, 415, 792, 873, 1242
BstMAI GTCTC 1 cut(s) 907
BstMBI GATC 6 cut(s) 133, 228, 412, 789, 870, 1239
BstMWI GCNNNNNNNGC 6 cut(s) 250, 341, 387, 508, 748, 989
BstNI CCWGG 1 cut(s) 974
BstNSI RCATGY 1 cut(s) 544
BstSCI CCNGG 1 cut(s) 972
BstSFI CTRYAG 1 cut(s) 803
BstUI CGCG 1 cut(s) 617
BstV1I GCAGC 4 cut(s) 331, 959, 1063, 1083
BstV2I GAAGAC 1 cut(s) 524
BstX2I RGATCY 1 cut(s) 789
BstYI RGATCY 1 cut(s) 789
BstZ17I GTATAC 2 cut(s) 206, 420
BsuRI GGCC 3 cut(s) 108, 426, 977
BtsCI GGATG 1 cut(s) 1016
BtsI GCAGTG 1 cut(s) 497
BtsIMutI CAGTG 2 cut(s) 497, 852
Cac8I GCNNGC 5 cut(s) 542, 640, 1005, 1170, 1203
CciI TCATGA 1 cut(s) 556
Cfr13I GGNCC 2 cut(s) 28, 535
Csp6I GTAC 2 cut(s) 621, 1059
CviAII CATG 5 cut(s) 504, 541, 557, 564, 606
CviQI GTAC 2 cut(s) 621, 1059
DdeI CTNAG 6 cut(s) 330, 574, 768, 979, 1103, 1113
DpnI GATC 6 cut(s) 135, 230, 414, 791, 872, 1241
DpnII GATC 6 cut(s) 133, 228, 412, 789, 870, 1239
EaeI YGGCCR 1 cut(s) 424
Eam1104I CTCTTC 1 cut(s) 72
EarI CTCTTC 1 cut(s) 72
Eco147I AGGCCT 2 cut(s) 108, 977
Eco24I GRGCYC 3 cut(s) 329, 973, 1273
Eco47I GGWCC 2 cut(s) 28, 535
EcoO109I RGGNCCY 1 cut(s) 28
EcoRII CCWGG 1 cut(s) 972
EcoT38I GRGCYC 3 cut(s) 329, 973, 1273
FaeI CATG 5 cut(s) 507, 544, 560, 567, 609
FatI CATG 5 cut(s) 503, 540, 556, 563, 605
FauNDI CATATG 1 cut(s) 429
FbaI TGATCA 1 cut(s) 228
FblI GTMKAC 2 cut(s) 205, 419
Fnu4HI GCNGC 4 cut(s) 345, 948, 1077, 1097
FokI GGATG 1 cut(s) 1003
FriOI GRGCYC 3 cut(s) 329, 973, 1273
Fsp4HI GCNGC 4 cut(s) 345, 948, 1077, 1097
FspBI CTAG 1 cut(s) 1010
GluI GCNGC 4 cut(s) 345, 948, 1077, 1097
GsuI CTGGAG 1 cut(s) 1254
HaeIII GGCC 3 cut(s) 108, 426, 977
HapII CCGG 1 cut(s) 248
Hin1II CATG 5 cut(s) 507, 544, 560, 567, 609
HindIII AAGCTT 3 cut(s) 149, 156, 1203
HinfI GANTC 7 cut(s) 74, 399, 560, 713, 838, 1031, 1127
HpaII CCGG 1 cut(s) 248
HphI GGTGA 3 cut(s) 619, 752, 902
Hpy166II GTNNAC 3 cut(s) 206, 420, 1257
Hpy188I TCNGA 8 cut(s) 236, 280, 396, 462, 718, 789, 1114, 1308
Hpy188III TCNNGA 4 cut(s) 71, 295, 557, 925
Hpy8I GTNNAC 3 cut(s) 206, 420, 1257
HpyAV CCTTC 1 cut(s) 152
HpyCH4III ACNGT 3 cut(s) 275, 1058, 1261
HpyCH4IV ACGT 1 cut(s) 526
HpyCH4V TGCA 5 cut(s) 663, 670, 805, 1099, 1222
HpyF10VI GCNNNNNNNGC 6 cut(s) 250, 341, 387, 508, 748, 989
HpyF3I CTNAG 6 cut(s) 330, 574, 768, 979, 1103, 1113
HpySE526I ACGT 1 cut(s) 526
Hsp92II CATG 5 cut(s) 507, 544, 560, 567, 609
Ksp22I TGATCA 1 cut(s) 228
Kzo9I GATC 6 cut(s) 133, 228, 412, 789, 870, 1239
LmnI GCTCC 1 cut(s) 739
Lsp1109I GCAGC 4 cut(s) 331, 959, 1063, 1083
LweI GCATC 2 cut(s) 992, 1108
MaeI CTAG 1 cut(s) 1010
MaeII ACGT 1 cut(s) 526
MaeIII GTNAC 6 cut(s) 495, 817, 835, 850, 1012, 1106
MalI GATC 6 cut(s) 135, 230, 414, 791, 872, 1241
MboI GATC 6 cut(s) 133, 228, 412, 789, 870, 1239
MboII GAAGA 7 cut(s) 59, 110, 368, 529, 784, 1279, 1282
MfeI CAATTG 1 cut(s) 909
MflI RGATCY 1 cut(s) 789
MhlI GDGCHC 4 cut(s) 329, 744, 973, 1273
MlsI TGGCCA 1 cut(s) 426
MluCI AATT 5 cut(s) 664, 909, 957, 1048, 1086
MluNI TGGCCA 1 cut(s) 426
MlyI GAGTC 2 cut(s) 832, 1136
MmeI TCCRAC 2 cut(s) 102, 111
Mox20I TGGCCA 1 cut(s) 426
MscI TGGCCA 1 cut(s) 426
MseI TTAA 4 cut(s) 465, 882, 1089, 1351
MslI CAYNNNNRTG 1 cut(s) 502
Msp20I TGGCCA 1 cut(s) 426
MspA1I CMGCKG 2 cut(s) 201, 1168
MspI CCGG 1 cut(s) 248
MspR9I CCNGG 1 cut(s) 974
MunI CAATTG 1 cut(s) 909
Mva1269I GAATGC 1 cut(s) 390
MvaI CCWGG 1 cut(s) 974
MvnI CGCG 1 cut(s) 617
MwoI GCNNNNNNNGC 6 cut(s) 250, 341, 387, 508, 748, 989
NdeI CATATG 1 cut(s) 429
NdeII GATC 6 cut(s) 133, 228, 412, 789, 870, 1239
NlaIII CATG 5 cut(s) 507, 544, 560, 567, 609
NlaIV GGNNCC 1 cut(s) 1067
NmuCI GTSAC 4 cut(s) 495, 817, 835, 850
NspI RCATGY 1 cut(s) 544
NspV TTCGAA 1 cut(s) 442
PaeI GCATGC 1 cut(s) 544
PagI TCATGA 1 cut(s) 556
PceI AGGCCT 2 cut(s) 108, 977
PctI GAATGC 1 cut(s) 390
PfeI GAWTC 5 cut(s) 74, 399, 560, 713, 1031
PflMI CCANNNNNTGG 1 cut(s) 999
PkrI GCNGC 4 cut(s) 346, 949, 1078, 1098
PleI GAGTC 2 cut(s) 832, 1135
PpsI GAGTC 2 cut(s) 832, 1135
Ppu21I YACGTR 1 cut(s) 527
PpuMI RGGWCCY 1 cut(s) 28
PshBI ATTAAT 1 cut(s) 882
Psp5II RGGWCCY 1 cut(s) 28
Psp6I CCWGG 1 cut(s) 972
PspGI CCWGG 1 cut(s) 972
PspN4I GGNNCC 1 cut(s) 1067
PspPI GGNCC 2 cut(s) 28, 535
PspPPI RGGWCCY 1 cut(s) 28
PstI CTGCAG 1 cut(s) 807
PsuI RGATCY 1 cut(s) 789
PvuII CAGCTG 2 cut(s) 201, 1168
RsaI GTAC 2 cut(s) 622, 1060
RsaNI GTAC 2 cut(s) 621, 1059
RseI CAYNNNNRTG 1 cut(s) 502
SaqAI TTAA 4 cut(s) 465, 882, 1089, 1351
SatI GCNGC 4 cut(s) 345, 948, 1077, 1097
Sau3AI GATC 6 cut(s) 133, 228, 412, 789, 870, 1239
Sau96I GGNCC 2 cut(s) 28, 535
SchI GAGTC 2 cut(s) 832, 1136
ScrFI CCNGG 1 cut(s) 974
SduI GDGCHC 4 cut(s) 329, 744, 973, 1273
SfaNI GCATC 2 cut(s) 992, 1108
SfcI CTRYAG 1 cut(s) 803
SfuI TTCGAA 1 cut(s) 442
SinI GGWCC 2 cut(s) 28, 535
SmiMI CAYNNNNRTG 1 cut(s) 502
SmlI CTYRAG 1 cut(s) 1070
SmoI CTYRAG 1 cut(s) 1070
SpeI ACTAGT 1 cut(s) 1009
SphI GCATGC 1 cut(s) 544
Sse9I AATT 5 cut(s) 664, 909, 957, 1048, 1086
SseBI AGGCCT 2 cut(s) 108, 977
SsiI CCGC 1 cut(s) 538
SspI AATATT 1 cut(s) 1248
SspMI CTAG 1 cut(s) 1010
StuI AGGCCT 2 cut(s) 108, 977
StyD4I CCNGG 1 cut(s) 972
TaaI ACNGT 3 cut(s) 275, 1058, 1261
TaiI ACGT 1 cut(s) 529
TaqI TCGA 6 cut(s) 72, 111, 442, 684, 1296, 1346
TasI AATT 5 cut(s) 664, 909, 957, 1048, 1086
TatI WGTACW 1 cut(s) 620
TfiI GAWTC 5 cut(s) 74, 399, 560, 713, 1031
Tru1I TTAA 4 cut(s) 465, 882, 1089, 1351
Tru9I TTAA 4 cut(s) 465, 882, 1089, 1351
TscAI CASTG 2 cut(s) 504, 859
TseFI GTSAC 4 cut(s) 495, 817, 835, 850
TseI GCWGC 4 cut(s) 344, 947, 1076, 1096
Tsp45I GTSAC 4 cut(s) 495, 817, 835, 850
TspDTI ATGAA 5 cut(s) 552, 868, 1023, 1164, 1282
TspRI CASTG 2 cut(s) 504, 859
Van91I CCANNNNNTGG 1 cut(s) 999
VpaK11BI GGWCC 2 cut(s) 28, 535
VspI ATTAAT 1 cut(s) 882
XapI RAATTY 2 cut(s) 957, 1048
XceI RCATGY 1 cut(s) 544
XmiI GTMKAC 2 cut(s) 205, 419
XspI CTAG 1 cut(s) 1010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.