Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004735

Physical Location & Seq

Genomic Coordinates Forward (+)

26069880 .. 26072245

Gene Structure 2366 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G69350.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2364 bp

ATGACGCAGTACATGCCATTGTTTAGATCATGCTCGAGCTTACGATTAGTAAGTCAGCTTCATGCTCACCTACTTGTCACCGGTCGTCTCCGTCGTGACCCTCTTCCAGTGACGAAGCTCATTGAATCATACGCTTTCATGGGTTCTCCAGATTCGTCTAGGCTCGTCTTTGAAGCTTTTCCTTACCCTGATTCCTTCATGTACGGTGTTCTCATCAAATGCAACGTCTGGTGTCATTTACTCGACGCCGCCATTGATCTGTACCATAGGTTGGTTTCGGAGACGACCCAAATCAGTAAGTTTGTGTTTCCGTCTGTTTTGAGGGCTTGTGCGGGTTCGAGGGAGCATCTGAGTGTTGGTGGGAAAGTCCATGGGAGAATTATCAAGGGCGGTGTAGACGACGACGCTGTGATAGAGACGTCTCTGTTGTGTATGTATGGTCAAACTGGGAATTTAAGTGATGCAGAGAAGGTGTTCGATGGAATGCCTGTGAGAGACCTTGTGGCTTGGAGTACTCTTGTTTCTAGCTGTCTCGAGAATGGTGAGGTGGTGAAAGCATTGAGGATGTTTAAGTGTATGGTGGATGATGGTGTTGAGCCCGATGCTGTGACGATGATCAGCGTTGTAGAAGGATGTGCTGAGCTTGGATGTTTGAGGATCGCTAGATCAGTTCATGGCCAGATAACGAGGAAAATGTTTGATTTAGATGAAACATTATGCAATTCTCTGCTTACAATGTACAGTAAGTGTGGGGATTTGCTTAGCTCGGAGAGAATCTTTGAGAAGATCGCCAAGAAGAATGCTGTTTCGTGGACAGCCATGATTTCTAGTTACAACCGTGGCGAGTTCTCTGAAAAGGCATTGAGAAGTTTCAGTGAGATGATAAAGTCTGGAATTGAGCCTAATTTGGTTACCCTTTATAGTGTTTTGAGCTCTTGTGGTTTGATAGGGTTGATTAGAGAAGGTAAGTCGGTCCATGGTTTTGCAGTTAGACGAGAGCTGGATCCGAATTACGAGTCGCTTTCTCTGGCCTTAGTTGAGCTGTACGCTGAATGCGGGAAACTAAGCGACTGTGAGACAGTTTTACGTGTGGTTAGTGATAGAAATATTGTCGCATGGAACTCTCTTATATCTCTATATGCACACAGAGGTATGGTGATTCAAGCATTGGGCTTGTTTAGACAGATGGTGACACAGAGAATAAAACCAGACGCATTCACTTTGGCCAGCTCAATCTCAGCATGCGAGAATGCTGGTTTAGTTCCGCTAGGGAAACAGATTCACGGACATGTTATAAGGACAGATGTTTCAGATGAGTTTGTGCAGAACTCGTTGATAGACATGTACTCTAAAAGCGGATCTGTGGATTCAGCAAGCACAGTGTTTAATCAAATCAAGCATAGGAGCGTTGTGACATGGAACTCAATGCTCTGCGGGTTTTCTCAGAATGGTAACTCTGTAGAGGCAATTAGCCTCTTCGACTATATGTATCATAGCTATCTCGAGATGAATGAAGTTACATTTTTGGCGGTTATTCAAGCATGCTCAAGCATAGGTTCACTGGAGAAAGGGAAATGGGTTCACCACAAGCTCATTATTTCCGGTTTAAAGGATCTTTTCACTGATACAGCTCTAATCGACATGTATGCCAAATGTGGAGATCTCAACGCAGCTGAAACAGTCTTCAGGGCCATGTCGAGTAGAAGCATTGTGTCATGGAGTAGTATGATCAATGCCTATGGAATGCACGGACGGATAGGATCCGCCATATCTACATTCAATCAAATGGTGGAATCCGGAACGAAACCTAACGAAGTTGTCTTCATGAACGTTCTCTCTGCTTGTGGTCACTCTGGTTCCGTTGAAGAAGGTAAGTACTACTTTAACTTGATGAAAAGTTTTGGCGTATCACCAAACTCAGAGCATTTCGCCTGTTTCATCGACCTTTTGAGCCGTTCTGGTGATCTCAAAGAAGCATATAGAACCATCAAGGAGATGCCTTTTCTAGCTGATGCTTCAGTTTGGGGTTCTCTGGTCAATGGATGCAGAATCCATCAGAAAATGGATATCATCAAAGCCATTAAAAACGATCTTTCGGATATTGTTACAGATGATACGGGATACTACACTCTCTTGTCCAATATATATGCTGAAGAAGGGGAATGGGAAGAATTCAGGAGACTGAGATCAGCTATGAAGAGTTCGAATCTTAAGAAAGTTCCTGGGTACAGCGCGATTGAGATTGATCAAAAAGTTTTCAGATTTGGAGCTGGTGAAGAAAATCGTATCCAAACAGATGAAATCTATAGGTTTCTTGGAAATTTGCAGAATCTAACGAATGAAGAACATGTCGTAGATTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

787

Amino Acids

87.49

Weight (kDa)

5.97

Isoelectric Point (pI)

40.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	166 - 213	1.3e-08	PPR repeat family
PPR_2	PF13041	266 - 312	7.6e-07	PPR repeat family
PPR_2	PF13041	368 - 415	3.1e-09	PPR repeat family
PPR	PF01535	371 - 398	7.5e-06	PPR repeat
PPR_2	PF13041	469 - 516	9.6e-10	PPR repeat family
PPR	PF01535	471 - 496	4.4e-06	PPR repeat
PPR_2	PF13041	569 - 606	6e-08	PPR repeat family
PPR	PF01535	571 - 600	1.1e-06	PPR repeat
E_motif	PF20431	694 - 747	2.8e-15	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004735)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69350
fragaria_vesca	FvH4_1g17060 FvH4_1g17060 FvH4_1g17060 FvH4_1g17060 FvH4_1g17060 FvH4_1g17060
malus_domestica	MD02G1180800.v1.1 MD16G1065000.v1.1
prunus_persica	Prupe.1G281900_v2.0.a1 Prupe.6G225400_v2.0.a1 Prupe.6G225400_v2.0.a1 Prupe.6G225400_v2.0.a1 Prupe.6G225400_v2.0.a1
pyrus_communis	pycom16g05680
rosa_chinensis	RchiOBHm_Chr2g0107231
rosa_laevigata	RLG00000017562
rosa_multiflora	Rmu_co8473113.1_g000001 Rmu_sc0027417.1_g000002 Rmu_sc0034517.1_g000001 Rmu_ssc0000269.1_g000007
rosa_roxburghii	Rroxscaffold_2G00136510 Rroxscaffold_2G00136520
rosa_rugosa	Rorug02G0141100 Rorug02G0141200
rosa_samantha	Rh2AG191900 Rh2BG203800 Rh2CG197100 Rh2DG199200
rosa_wichuraiana	Rw2G015170 Rw2G015180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1295
AatII	GACGTC	1 cut(s)	422
AccB7I	CCANNNNNTGG	1 cut(s)	271
AccI	GTMKAC	1 cut(s)	396
AccII	CGCG	1 cut(s)	2234
AccIII	TCCGGA	1 cut(s)	1796
AciI	CCGC	9 cut(s)	249, 332, 390, 1056, 1265, 1356, 1434, 1529, 1764
AclI	AACGTT	1 cut(s)	1830
AclWI	GGATC	7 cut(s)	665, 998, 1011, 1366, 1620, 1755, 1768
AcoI	YGGCCR	2 cut(s)	676, 1224
AcsI	RAATTY	3 cut(s)	451, 2171, 2320
AcuI	CTGAAG	3 cut(s)	1669, 2001, 2172
AcyI	GRCGYC	2 cut(s)	246, 419
AfaI	GTAC	9 cut(s)	11, 203, 263, 514, 740, 1046, 1346, 1877, 2228
AfiI	CCNNNNNNNGG	1 cut(s)	271
AflII	CTTAAG	1 cut(s)	2210
AflIII	ACRYGT	5 cut(s)	1087, 1288, 1341, 1641, 2347
AgeI	ACCGGT	1 cut(s)	80
AgsI	TTSAA	6 cut(s)	125, 173, 1163, 1538, 1780, 1865
AjnI	CCWGG	1 cut(s)	2221
Alw21I	GWGCWC	1 cut(s)	935
Alw26I	GTCTC	8 cut(s)	92, 275, 410, 426, 489, 536, 1070, 2173
AlwI	GGATC	7 cut(s)	665, 998, 1011, 1366, 1620, 1755, 1768
Ama87I	CYCGRG	3 cut(s)	34, 533, 1502
Aor13HI	TCCGGA	1 cut(s)	1796
AoxI	GGCC	4 cut(s)	676, 1029, 1224, 1689
ApeKI	GCWGC	1 cut(s)	1670
ApoI	RAATTY	3 cut(s)	451, 2171, 2320
ArsI	GACNNNNNNTTYG	4 cut(s)	210, 242, 1001, 1033
AsiGI	ACCGGT	1 cut(s)	80
Asp700I	GAANNNNTTC	2 cut(s)	177, 473
AspLEI	GCGC	1 cut(s)	2234
AspS9I	GGNCC	2 cut(s)	973, 1689
AsuII	TTCGAA	1 cut(s)	2204
AvaI	CYCGRG	3 cut(s)	34, 533, 1502
AvaII	GGWCC	1 cut(s)	973
BalI	TGGCCA	2 cut(s)	678, 1226
BamHI	GGATCC	2 cut(s)	1003, 1760
BanII	GRGCYC	2 cut(s)	600, 935
BarI	GAAGNNNNNNTAC	2 cut(s)	42, 74
BbsI	GAAGAC	2 cut(s)	1675, 1813
Bbv12I	GWGCWC	1 cut(s)	935
BbvI	GCAGC	1 cut(s)	1682
BccI	CCATC	5 cut(s)	473, 581, 1180, 1994, 2061
BceAI	ACGGC	1 cut(s)	1938
BcgI	CGANNNNNNTGC	2 cut(s)	1628, 1662
BciT130I	CCWGG	1 cut(s)	2223
BciVI	GTATCC	2 cut(s)	2114, 2297
BclI	TGATCA	3 cut(s)	615, 1728, 2245
BcoDI	GTCTC	8 cut(s)	92, 275, 410, 426, 489, 536, 1070, 2173
BfaI	CTAG	6 cut(s)	159, 525, 663, 828, 1268, 2006
BfmI	CTRYAG	2 cut(s)	1458, 2305
BfrI	CTTAAG	1 cut(s)	2210
BfuI	GTATCC	2 cut(s)	2114, 2297
BglII	AGATCT	1 cut(s)	1660
BisI	GCNGC	2 cut(s)	249, 1671
BlpI	GCTNAGC	2 cut(s)	639, 761
BlsI	GCNGC	2 cut(s)	250, 1672
BmcAI	AGTACT	2 cut(s)	514, 1877
Bme1390I	CCNGG	1 cut(s)	2223
Bme18I	GGWCC	1 cut(s)	973
BmeT110I	CYCGRG	3 cut(s)	34, 533, 1502
BmgT120I	GGNCC	2 cut(s)	973, 1689
BmiI	GGNNCC	3 cut(s)	1005, 1762, 1858
BmrFI	CCNGG	1 cut(s)	2223
BmrI	ACTGGG	1 cut(s)	456
BmsI	GCATC	6 cut(s)	355, 451, 592, 1986, 2002, 2033
BmuI	ACTGGG	1 cut(s)	456
BpiI	GAAGAC	2 cut(s)	1675, 1813
BpmI	CTGGAG	2 cut(s)	132, 1583
Bpu1102I	GCTNAGC	2 cut(s)	639, 761
Bpu14I	TTCGAA	1 cut(s)	2204
BpuEI	CTTGAG	1 cut(s)	1531
BsaAI	YACGTR	1 cut(s)	1088
BsaHI	GRCGYC	2 cut(s)	246, 419
BsaI	GGTCTC	1 cut(s)	489
BsaJI	CCNNGG	4 cut(s)	370, 838, 976, 2222
BsaWI	WCCGGW	3 cut(s)	80, 1601, 1796
Bsc4I	CCNNNNNNNGG	1 cut(s)	271
Bse118I	RCCGGY	1 cut(s)	80
Bse1I	ACTGG	3 cut(s)	107, 451, 1566
BseAI	TCCGGA	1 cut(s)	1796
BseBI	CCWGG	1 cut(s)	2223
BseDI	CCNNGG	4 cut(s)	370, 838, 976, 2222
BseGI	GGATG	5 cut(s)	570, 589, 638, 653, 2048
BseLI	CCNNNNNNNGG	1 cut(s)	271
BseMII	CTCAG	6 cut(s)	341, 630, 1251, 1457, 1932, 2174
BseNI	ACTGG	3 cut(s)	107, 451, 1566
BseXI	GCAGC	1 cut(s)	1682
BsgI	GTGCAG	1 cut(s)	1343
Bsh1236I	CGCG	1 cut(s)	2234
Bsh1285I	CGRYCG	1 cut(s)	85
BshFI	GGCC	4 cut(s)	678, 1031, 1226, 1691
BshTI	ACCGGT	1 cut(s)	80
BsiEI	CGRYCG	1 cut(s)	85
BsiHKAI	GWGCWC	1 cut(s)	935
BsiHKCI	CYCGRG	3 cut(s)	34, 533, 1502
BsiSI	CCGG	3 cut(s)	81, 1602, 1797
BslI	CCNNNNNNNGG	1 cut(s)	271
BsmAI	GTCTC	8 cut(s)	92, 275, 410, 426, 489, 536, 1070, 2173
BsmBI	CGTCTC	4 cut(s)	92, 275, 410, 426
BsmI	GAATGC	6 cut(s)	489, 805, 1058, 1214, 1255, 1749
BsnI	GGCC	4 cut(s)	678, 1031, 1226, 1691
Bso31I	GGTCTC	1 cut(s)	489
BsoBI	CYCGRG	3 cut(s)	34, 533, 1502
Bsp119I	TTCGAA	1 cut(s)	2204
Bsp1286I	GDGCHC	2 cut(s)	600, 935
Bsp13I	TCCGGA	1 cut(s)	1796
Bsp1407I	TGTACA	1 cut(s)	738
Bsp1720I	GCTNAGC	2 cut(s)	639, 761
Bsp19I	CCATGG	2 cut(s)	370, 976
BspACI	CCGC	9 cut(s)	249, 332, 390, 1056, 1265, 1356, 1434, 1529, 1764
BspANI	GGCC	4 cut(s)	678, 1031, 1226, 1691
BspCNI	CTCAG	6 cut(s)	342, 631, 1250, 1456, 1931, 2175
BspEI	TCCGGA	1 cut(s)	1796
BspFNI	CGCG	1 cut(s)	2234
BspHI	TCATGA	1 cut(s)	1824
BspLI	GGNNCC	3 cut(s)	1005, 1762, 1858
BspPI	GGATC	7 cut(s)	665, 998, 1011, 1366, 1620, 1755, 1768
BspT104I	TTCGAA	1 cut(s)	2204
BspTI	CTTAAG	1 cut(s)	2210
BspTNI	GGTCTC	1 cut(s)	489
BsrFI	RCCGGY	1 cut(s)	80
BsrGI	TGTACA	1 cut(s)	738
BsrI	ACTGG	3 cut(s)	107, 451, 1566
BssAI	RCCGGY	1 cut(s)	80
BssECI	CCNNGG	4 cut(s)	370, 838, 976, 2222
BssNI	GRCGYC	2 cut(s)	246, 419
BssT1I	CCWWGG	2 cut(s)	370, 976
Bst2UI	CCWGG	1 cut(s)	2223
Bst4CI	ACNGT	7 cut(s)	206, 743, 839, 1073, 1081, 1381, 1681
Bst6I	CTCTTC	3 cut(s)	108, 1481, 2192
BstACI	GRCGYC	2 cut(s)	246, 419
BstAFI	CTTAAG	1 cut(s)	2210
BstAPI	GCANNNNNTGC	1 cut(s)	13
BstAUI	TGTACA	1 cut(s)	738
BstBAI	YACGTR	1 cut(s)	1088
BstBI	TTCGAA	1 cut(s)	2204
BstC8I	GCNNGC	4 cut(s)	1228, 1243, 1375, 1543
BstDSI	CCRYGG	3 cut(s)	370, 838, 976
BstEII	GGTNACC	1 cut(s)	910
BstF5I	GGATG	5 cut(s)	570, 589, 638, 653, 2048
BstFNI	CGCG	1 cut(s)	2234
BstHHI	GCGC	1 cut(s)	2234
BstMAI	GTCTC	8 cut(s)	92, 275, 410, 426, 489, 536, 1070, 2173
BstMCI	CGRYCG	1 cut(s)	85
BstMWI	GCNNNNNNNGC	1 cut(s)	13
BstNI	CCWGG	1 cut(s)	2223
BstNSI	RCATGY	7 cut(s)	16, 1245, 1292, 1345, 1545, 1645, 2351
BstPI	GGTNACC	1 cut(s)	910
BstSCI	CCNGG	1 cut(s)	2221
BstSFI	CTRYAG	2 cut(s)	1458, 2305
BstUI	CGCG	1 cut(s)	2234
BstV1I	GCAGC	1 cut(s)	1682
BstV2I	GAAGAC	2 cut(s)	1675, 1813
BstX2I	RGATCY	5 cut(s)	1003, 1358, 1612, 1660, 1760
BstYI	RGATCY	5 cut(s)	1003, 1358, 1612, 1660, 1760
BsuI	GTATCC	2 cut(s)	2114, 2297
BsuRI	GGCC	4 cut(s)	678, 1031, 1226, 1691
BtgI	CCRYGG	3 cut(s)	370, 838, 976
BtsCI	GGATG	5 cut(s)	570, 589, 638, 653, 2048
BtsIMutI	CAGTG	5 cut(s)	114, 880, 1386, 1559, 1620
Cac8I	GCNNGC	4 cut(s)	1228, 1243, 1375, 1543
CciI	TCATGA	1 cut(s)	1824
CfoI	GCGC	1 cut(s)	2234
Cfr10I	RCCGGY	1 cut(s)	80
Cfr13I	GGNCC	2 cut(s)	973, 1689
CseI	GACGC	4 cut(s)	13, 254, 413, 1220
Csp6I	GTAC	9 cut(s)	10, 202, 262, 513, 739, 1045, 1345, 1876, 2227
CspAI	ACCGGT	1 cut(s)	80
CviQI	GTAC	9 cut(s)	10, 202, 262, 513, 739, 1045, 1345, 1876, 2227
DraI	TTTAAA	1 cut(s)	1608
EaeI	YGGCCR	2 cut(s)	676, 1224
Eam1104I	CTCTTC	3 cut(s)	108, 1481, 2192
EarI	CTCTTC	3 cut(s)	108, 1481, 2192
EciI	GGCGGA	1 cut(s)	1753
Ecl136II	GAGCTC	1 cut(s)	933
Eco130I	CCWWGG	2 cut(s)	370, 976
Eco24I	GRGCYC	2 cut(s)	600, 935
Eco31I	GGTCTC	1 cut(s)	489
Eco32I	GATATC	1 cut(s)	2068
Eco47I	GGWCC	1 cut(s)	973
Eco53kI	GAGCTC	1 cut(s)	933
Eco57I	CTGAAG	3 cut(s)	1669, 2001, 2172
Eco88I	CYCGRG	3 cut(s)	34, 533, 1502
Eco91I	GGTNACC	1 cut(s)	910
EcoICRI	GAGCTC	1 cut(s)	933
EcoO65I	GGTNACC	1 cut(s)	910
EcoRI	GAATTC	1 cut(s)	2171
EcoRII	CCWGG	1 cut(s)	2221
EcoRV	GATATC	1 cut(s)	2068
EcoT14I	CCWWGG	2 cut(s)	370, 976
EcoT38I	GRGCYC	2 cut(s)	600, 935
ErhI	CCWWGG	2 cut(s)	370, 976
Esp3I	CGTCTC	4 cut(s)	92, 275, 410, 426
FalI	AAGNNNNNCTT	2 cut(s)	1865, 1897
FauI	CCCGC	3 cut(s)	325, 1049, 1427
FbaI	TGATCA	3 cut(s)	615, 1728, 2245
FblI	GTMKAC	1 cut(s)	396
Fnu4HI	GCNGC	2 cut(s)	249, 1671
FokI	GGATG	5 cut(s)	577, 596, 645, 660, 2055
FriOI	GRGCYC	2 cut(s)	600, 935
Fsp4HI	GCNGC	2 cut(s)	249, 1671
FspBI	CTAG	6 cut(s)	159, 525, 663, 828, 1268, 2006
GlaI	GCGC	1 cut(s)	2233
GluI	GCNGC	2 cut(s)	249, 1671
GsuI	CTGGAG	2 cut(s)	132, 1583
HaeIII	GGCC	4 cut(s)	678, 1031, 1226, 1691
HapII	CCGG	3 cut(s)	81, 1602, 1797
HgaI	GACGC	4 cut(s)	13, 254, 413, 1220
HhaI	GCGC	1 cut(s)	2234
Hin1I	GRCGYC	2 cut(s)	246, 419
Hin6I	GCGC	1 cut(s)	2232
HinP1I	GCGC	1 cut(s)	2232
HindIII	AAGCTT	1 cut(s)	174
HpaII	CCGG	3 cut(s)	81, 1602, 1797
Hpy166II	GTNNAC	4 cut(s)	397, 813, 1559, 1582
Hpy8I	GTNNAC	4 cut(s)	397, 813, 1559, 1582
Hpy99I	CGWCG	4 cut(s)	96, 248, 404, 407
HpyAV	CCTTC	6 cut(s)	205, 463, 623, 956, 1862, 2150
HpyCH4III	ACNGT	7 cut(s)	206, 743, 839, 1073, 1081, 1381, 1681
HpyCH4IV	ACGT	4 cut(s)	225, 419, 1087, 1830
HpyCH4V	TGCA	9 cut(s)	222, 464, 720, 986, 1142, 1324, 1747, 2046, 2326
HpyF10VI	GCNNNNNNNGC	1 cut(s)	13
HpySE526I	ACGT	4 cut(s)	225, 419, 1087, 1830
Hsp92I	GRCGYC	2 cut(s)	246, 419
HspAI	GCGC	1 cut(s)	2232
Kpn2I	TCCGGA	1 cut(s)	1796
Ksp22I	TGATCA	3 cut(s)	615, 1728, 2245
LmnI	GCTCC	3 cut(s)	343, 1404, 2267
Lsp1109I	GCAGC	1 cut(s)	1682
LweI	GCATC	6 cut(s)	355, 451, 592, 1986, 2002, 2033
MaeI	CTAG	6 cut(s)	159, 525, 663, 828, 1268, 2006
MaeII	ACGT	4 cut(s)	225, 419, 1087, 1830
MflI	RGATCY	5 cut(s)	1003, 1358, 1612, 1660, 1760
MhlI	GDGCHC	2 cut(s)	600, 935
MlsI	TGGCCA	2 cut(s)	678, 1226
MluCI	AATT	9 cut(s)	378, 451, 721, 894, 904, 1009, 1467, 2171, 2320
MluNI	TGGCCA	2 cut(s)	678, 1226
MlyI	GAGTC	1 cut(s)	1025
Mox20I	TGGCCA	2 cut(s)	678, 1226
MroI	TCCGGA	1 cut(s)	1796
MroXI	GAANNNNTTC	2 cut(s)	177, 473
MscI	TGGCCA	2 cut(s)	678, 1226
MseI	TTAA	7 cut(s)	455, 570, 1386, 1607, 1884, 2082, 2211
MslI	CAYNNNNRTG	2 cut(s)	351, 1287
Msp20I	TGGCCA	2 cut(s)	678, 1226
MspA1I	CMGCKG	1 cut(s)	1673
MspCI	CTTAAG	1 cut(s)	2210
MspI	CCGG	3 cut(s)	81, 1602, 1797
MspR9I	CCNGG	1 cut(s)	2223
Mva1269I	GAATGC	6 cut(s)	489, 805, 1058, 1214, 1255, 1749
MvaI	CCWGG	1 cut(s)	2223
MvnI	CGCG	1 cut(s)	2234
MwoI	GCNNNNNNNGC	1 cut(s)	13
NcoI	CCATGG	2 cut(s)	370, 976
NlaIV	GGNNCC	3 cut(s)	1005, 1762, 1858
NmuCI	GTSAC	7 cut(s)	76, 95, 109, 607, 1189, 1411, 1847
NspI	RCATGY	7 cut(s)	16, 1245, 1292, 1345, 1545, 1645, 2351
NspV	TTCGAA	1 cut(s)	2204
PaeI	GCATGC	2 cut(s)	1245, 1545
PaeR7I	CTCGAG	3 cut(s)	34, 533, 1502
PagI	TCATGA	1 cut(s)	1824
PciI	ACATGT	4 cut(s)	1288, 1341, 1641, 2347
PcsI	WCGNNNNNNNCGW	1 cut(s)	91
PctI	GAATGC	6 cut(s)	489, 805, 1058, 1214, 1255, 1749
PdmI	GAANNNNTTC	2 cut(s)	177, 473
PflMI	CCANNNNNTGG	1 cut(s)	271
PinAI	ACCGGT	1 cut(s)	80
PkrI	GCNGC	2 cut(s)	250, 1672
PleI	GAGTC	1 cut(s)	1024
PpsI	GAGTC	1 cut(s)	1024
Ppu21I	YACGTR	1 cut(s)	1088
PscI	ACATGT	4 cut(s)	1288, 1341, 1641, 2347
PsiI	TTATAA	1 cut(s)	1295
Psp124BI	GAGCTC	1 cut(s)	935
Psp1406I	AACGTT	1 cut(s)	1830
Psp6I	CCWGG	1 cut(s)	2221
PspEI	GGTNACC	1 cut(s)	910
PspGI	CCWGG	1 cut(s)	2221
PspN4I	GGNNCC	3 cut(s)	1005, 1762, 1858
PspPI	GGNCC	2 cut(s)	973, 1689
PspXI	VCTCGAGB	1 cut(s)	34
PsuI	RGATCY	5 cut(s)	1003, 1358, 1612, 1660, 1760
PvuII	CAGCTG	1 cut(s)	1673
RsaI	GTAC	9 cut(s)	11, 203, 263, 514, 740, 1046, 1346, 1877, 2228
RsaNI	GTAC	9 cut(s)	10, 202, 262, 513, 739, 1045, 1345, 1876, 2227
RseI	CAYNNNNRTG	2 cut(s)	351, 1287
SacI	GAGCTC	1 cut(s)	935
SaqAI	TTAA	7 cut(s)	455, 570, 1386, 1607, 1884, 2082, 2211
SatI	GCNGC	2 cut(s)	249, 1671
Sau96I	GGNCC	2 cut(s)	973, 1689
ScaI	AGTACT	2 cut(s)	514, 1877
SchI	GAGTC	1 cut(s)	1025
ScrFI	CCNGG	1 cut(s)	2223
SduI	GDGCHC	2 cut(s)	600, 935
SfaNI	GCATC	6 cut(s)	355, 451, 592, 1986, 2002, 2033
SfcI	CTRYAG	2 cut(s)	1458, 2305
Sfr274I	CTCGAG	3 cut(s)	34, 533, 1502
SfuI	TTCGAA	1 cut(s)	2204
SinI	GGWCC	1 cut(s)	973
SlaI	CTCGAG	3 cut(s)	34, 533, 1502
SmiMI	CAYNNNNRTG	2 cut(s)	351, 1287
SmlI	CTYRAG	5 cut(s)	34, 533, 1502, 1546, 2210
SmoI	CTYRAG	5 cut(s)	34, 533, 1502, 1546, 2210
SphI	GCATGC	2 cut(s)	1245, 1545
Sse9I	AATT	9 cut(s)	378, 451, 721, 894, 904, 1009, 1467, 2171, 2320
SsiI	CCGC	9 cut(s)	249, 332, 390, 1056, 1265, 1356, 1434, 1529, 1764
SspI	AATATT	1 cut(s)	1108
SspMI	CTAG	6 cut(s)	159, 525, 663, 828, 1268, 2006
SstI	GAGCTC	1 cut(s)	935
StyD4I	CCNGG	1 cut(s)	2221
StyI	CCWWGG	2 cut(s)	370, 976
TaaI	ACNGT	7 cut(s)	206, 743, 839, 1073, 1081, 1381, 1681
TaiI	ACGT	4 cut(s)	228, 422, 1090, 1833
TaqII	GACCGA	1 cut(s)	961
TasI	AATT	9 cut(s)	378, 451, 721, 894, 904, 1009, 1467, 2171, 2320
TatI	WGTACW	5 cut(s)	9, 512, 738, 1344, 1875
TauI	GCSGC	1 cut(s)	251
Tru1I	TTAA	7 cut(s)	455, 570, 1386, 1607, 1884, 2082, 2211
Tru9I	TTAA	7 cut(s)	455, 570, 1386, 1607, 1884, 2082, 2211
TscAI	CASTG	5 cut(s)	114, 880, 1386, 1566, 1627
TseFI	GTSAC	7 cut(s)	76, 95, 109, 607, 1189, 1411, 1847
TseI	GCWGC	1 cut(s)	1670
Tsp45I	GTSAC	7 cut(s)	76, 95, 109, 607, 1189, 1411, 1847
TspGWI	ACGGA	6 cut(s)	80, 300, 1299, 1764, 1768, 1849
TspRI	CASTG	5 cut(s)	114, 880, 1386, 1566, 1627
Van91I	CCANNNNNTGG	1 cut(s)	271
Vha464I	CTTAAG	1 cut(s)	2210
VpaK11BI	GGWCC	1 cut(s)	973
XapI	RAATTY	3 cut(s)	451, 2171, 2320
XceI	RCATGY	7 cut(s)	16, 1245, 1292, 1345, 1545, 1645, 2351
XhoI	CTCGAG	3 cut(s)	34, 533, 1502
XmiI	GTMKAC	1 cut(s)	396
XmnI	GAANNNNTTC	2 cut(s)	177, 473
XspI	CTAG	6 cut(s)	159, 525, 663, 828, 1268, 2006
ZraI	GACGTC	1 cut(s)	420
ZrmI	AGTACT	2 cut(s)	514, 1877

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.