Rorug02G0141200

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004735
Physical Location & Seq
Reverse (-)
12404476 .. 12406758
2283 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0141200.1

Sequence Viewer

Length: 1089 bp
ATGGCGGATTACTTACCTGAAGACGTGATAGTTCGAATCCTGAAGAGGTTGCAGATCAAATCCCTAATCCGCTTCACCTGCGTTTCGAAACGCTGGCGTTCCATCATATTGGCCGACCCAGAATTCGCCAAGACCCAATTCCAAATAGCCTCTCAACACCAAACCCCCCGTCACAGGCTCCTCATCTCCACCGCCTGTGAATCTGAATGCAACTCACTAGACTTGGATGCGCCGCGGTCGCCGTTGGGATACAATTCTTCGGTTAGGTGCCCATTCACGCAACCGGGGGGCCGTCATGTCGCACTACTGTGCTCCTGCAATGGTTTGATACTCGCAACTCTTTATAAAGAAGAACACAGCATGTATATCTGGAACCCATCAACTCAATTCTTCAAAAAATTACCTGACCCAGCTGATCGTCCCGCAAAAACTCGTCTATTCGGCTGTGGTTTTGGCTATTTGTCGGCCACTGACGACTACAAAGTTGTAGCCGACTTCAGAGTCAGTTCTGTTGATGAGGTGAAGGGCAAGGAGATGCACATCTTCTCGTCAAAAGCCAACATTTGGAAAAGGACTGAAGCCCCCCCTCTCTTCGACTACAAGTTTGAATATGAGGGGACTCTTTCAAATGAGGCACTTCATTGGCTGGATGAACTTGGCATCTTTGCTTTTGATCTAGCAAAGGAGGAGTTCCAAAAAATGCCACTGCCTATTCAAGATGGTAAGGTTAAGTATCTCGGGGCTTTCGGAGACTGTCTGTGTGCATTTGATTGTGCAGAATTTTATACTGCTGGCTCCATTGATTTGTGGGTGATGAAAAAATATGGCGTGGCTGACTCATGGACCAAGCTCTTTAACTTAAAGGTTTCCGATCAGCCTCAGAATATAATGTATTTTTTGCCAGTCTCTGTAATGGAAACTAGTACATTTATGTACACAATGACTATACTAGGGAAACAGAAGTTTCTTAATTTGATAAGGATTGGTCATACGAAACAGAAGCCCAAAATGCATCTGTTGAGCAGGAATACAGATCTCATGGAAATGGTCGAATATGAAGAGACTCTACTTTGGCTTGCTGCCCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

362

Amino Acids

41.52

Weight (kDa)

7.53

Isoelectric Point (pI)

51.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 3 - 39 6e-08 F-box-like
F-box PF00646 5 - 37 8.3e-10 F-box domain
b-prop_At3g26010-like PF24750 76 - 239 1.2e-07 F-box protein At3g26010-like, beta-propeller
FBA_3 PF08268 88 - 317 3e-11 F-box associated beta propeller domain
FBA_1 PF07734 100 - 299 3.2e-20 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 345
AarI CACCTGC 1 cut(s) 86
AasI GACNNNNNNGTC 1 cut(s) 500
Acc36I ACCTGC 1 cut(s) 86
AccB1I GGYRCC 1 cut(s) 267
AccB7I CCANNNNNTGG 1 cut(s) 564
AccII CGCG 1 cut(s) 235
AciI CCGC 6 cut(s) 5, 70, 192, 233, 235, 423
AcoI YGGCCR 2 cut(s) 111, 465
AcsI RAATTY 2 cut(s) 122, 779
AcuI CTGAAG 4 cut(s) 39, 62, 481, 597
AfaI GTAC 2 cut(s) 925, 935
AfiI CCNNNNNNNGG 2 cut(s) 174, 564
AgsI TTSAA 4 cut(s) 394, 608, 627, 716
AhlI ACTAGT 1 cut(s) 920
AjiI CACGTC 1 cut(s) 25
AjuI GAANNNNNNNTTGG 2 cut(s) 122, 154
AleI CACNNNNGTG 1 cut(s) 307
AluBI AGCT 2 cut(s) 413, 850
AluI AGCT 2 cut(s) 413, 850
Alw21I GWGCWC 1 cut(s) 314
Alw26I GTCTC 3 cut(s) 744, 910, 1055
AlwNI CAGNNNCTG 1 cut(s) 908
Ama87I CYCGRG 1 cut(s) 737
AoxI GGCC 3 cut(s) 111, 289, 465
ApeKI GCWGC 1 cut(s) 1079
ApoI RAATTY 2 cut(s) 122, 779
ArsI GACNNNNNNTTYG 6 cut(s) 107, 139, 154, 186, 587, 619
AspLEI GCGC 1 cut(s) 232
AspS9I GGNCC 2 cut(s) 289, 843
AsuC2I CCSGG 1 cut(s) 285
AsuHPI GGTGA 3 cut(s) 67, 532, 823
AsuII TTCGAA 2 cut(s) 34, 86
AvaI CYCGRG 1 cut(s) 737
AvaII GGWCC 1 cut(s) 843
BaeGI GKGCMC 1 cut(s) 272
BanI GGYRCC 1 cut(s) 267
BarI GAAGNNNNNNTAC 2 cut(s) 1050, 1082
BbsI GAAGAC 1 cut(s) 27
Bbv12I GWGCWC 1 cut(s) 314
BbvI GCAGC 1 cut(s) 1066
BccI CCATC 3 cut(s) 110, 385, 713
BceAI ACGGC 2 cut(s) 226, 276
BciVI GTATCC 1 cut(s) 242
BcnI CCSGG 1 cut(s) 285
BcoDI GTCTC 3 cut(s) 744, 910, 1055
BcuI ACTAGT 1 cut(s) 920
BfaI CTAG 4 cut(s) 218, 677, 921, 950
BfuAI ACCTGC 1 cut(s) 86
BfuI GTATCC 1 cut(s) 242
BglII AGATCT 1 cut(s) 1033
BisI GCNGC 2 cut(s) 233, 1080
BlsI GCNGC 2 cut(s) 234, 1081
Bme1390I CCNGG 1 cut(s) 285
Bme18I GGWCC 1 cut(s) 843
BmeT110I CYCGRG 1 cut(s) 737
BmgBI CACGTC 1 cut(s) 25
BmgT120I GGNCC 2 cut(s) 289, 843
BmiI GGNNCC 5 cut(s) 179, 269, 290, 374, 796
BmrFI CCNGG 1 cut(s) 285
BmsI GCATC 4 cut(s) 217, 525, 669, 1021
BpiI GAAGAC 1 cut(s) 27
Bpu14I TTCGAA 2 cut(s) 34, 86
BpuMI CCSGG 1 cut(s) 285
BsaBI GATNNNNATC 1 cut(s) 539
BsaJI CCNNGG 2 cut(s) 233, 284
Bsc4I CCNNNNNNNGG 2 cut(s) 174, 564
Bse1I ACTGG 1 cut(s) 902
Bse3DI GCAATG 1 cut(s) 325
Bse8I GATNNNNATC 1 cut(s) 539
BseDI CCNNGG 2 cut(s) 233, 284
BseGI GGATG 2 cut(s) 232, 655
BseJI GATNNNNATC 1 cut(s) 539
BseLI CCNNNNNNNGG 2 cut(s) 174, 564
BseMI GCAATG 1 cut(s) 325
BseMII CTCAG 1 cut(s) 893
BseNI ACTGG 1 cut(s) 902
BseRI GAGGAG 2 cut(s) 170, 701
BseSI GKGCMC 1 cut(s) 272
BseXI GCAGC 1 cut(s) 1066
BseYI CCCAGC 1 cut(s) 409
BsgI GTGCAG 1 cut(s) 795
Bsh1236I CGCG 1 cut(s) 235
Bsh1285I CGRYCG 1 cut(s) 239
BshFI GGCC 3 cut(s) 113, 291, 467
BshNI GGYRCC 1 cut(s) 267
BsiEI CGRYCG 1 cut(s) 239
BsiHKAI GWGCWC 1 cut(s) 314
BsiHKCI CYCGRG 1 cut(s) 737
BsiSI CCGG 1 cut(s) 284
BslFI GGGAC 2 cut(s) 405, 631
BslI CCNNNNNNNGG 2 cut(s) 174, 564
BsmAI GTCTC 3 cut(s) 744, 910, 1055
BsmFI GGGAC 2 cut(s) 405, 631
BsmI GAATGC 1 cut(s) 212
BsnI GGCC 3 cut(s) 113, 291, 467
BsoBI CYCGRG 1 cut(s) 737
Bsp119I TTCGAA 2 cut(s) 34, 86
Bsp1286I GDGCHC 2 cut(s) 272, 314
Bsp1407I TGTACA 1 cut(s) 933
Bsp143I GATC 5 cut(s) 54, 415, 673, 871, 1033
BspACI CCGC 6 cut(s) 5, 70, 192, 233, 235, 423
BspANI GGCC 3 cut(s) 113, 291, 467
BspCNI CTCAG 1 cut(s) 892
BspFNI CGCG 1 cut(s) 235
BspLI GGNNCC 5 cut(s) 179, 269, 290, 374, 796
BspMI ACCTGC 1 cut(s) 86
BspT104I TTCGAA 2 cut(s) 34, 86
BspT107I GGYRCC 1 cut(s) 267
BsrDI GCAATG 1 cut(s) 325
BsrGI TGTACA 1 cut(s) 933
BsrI ACTGG 1 cut(s) 902
BssECI CCNNGG 2 cut(s) 233, 284
BssMI GATC 5 cut(s) 54, 415, 673, 871, 1033
Bst4CI ACNGT 2 cut(s) 309, 755
Bst6I CTCTTC 3 cut(s) 38, 596, 1053
BstAUI TGTACA 1 cut(s) 933
BstBI TTCGAA 2 cut(s) 34, 86
BstC8I GCNNGC 3 cut(s) 95, 793, 1077
BstDEI CTNAG 1 cut(s) 879
BstDSI CCRYGG 1 cut(s) 233
BstF5I GGATG 2 cut(s) 232, 655
BstFNI CGCG 1 cut(s) 235
BstHHI GCGC 1 cut(s) 232
BstKTI GATC 5 cut(s) 57, 418, 676, 874, 1036
BstMAI GTCTC 3 cut(s) 744, 910, 1055
BstMBI GATC 5 cut(s) 54, 415, 673, 871, 1033
BstMCI CGRYCG 1 cut(s) 239
BstMWI GCNNNNNNNGC 3 cut(s) 78, 238, 1009
BstNSI RCATGY 1 cut(s) 364
BstSCI CCNGG 1 cut(s) 283
BstSLI GKGCMC 1 cut(s) 272
BstUI CGCG 1 cut(s) 235
BstV1I GCAGC 1 cut(s) 1066
BstV2I GAAGAC 1 cut(s) 27
BstX2I RGATCY 1 cut(s) 1033
BstXI CCANNNNNNTGG 1 cut(s) 109
BstYI RGATCY 1 cut(s) 1033
BsuI GTATCC 1 cut(s) 242
BsuRI GGCC 3 cut(s) 113, 291, 467
BtgI CCRYGG 1 cut(s) 233
BtrI CACGTC 1 cut(s) 25
BtsCI GGATG 2 cut(s) 232, 655
BtsI GCAGTG 1 cut(s) 704
BtsIMutI CAGTG 2 cut(s) 468, 704
BveI ACCTGC 1 cut(s) 86
Cac8I GCNNGC 3 cut(s) 95, 793, 1077
CaiI CAGNNNCTG 1 cut(s) 908
CfoI GCGC 1 cut(s) 232
Cfr13I GGNCC 2 cut(s) 289, 843
Cfr42I CCGCGG 1 cut(s) 236
Csp6I GTAC 2 cut(s) 924, 934
CviAII CATG 4 cut(s) 296, 361, 840, 1039
CviQI GTAC 2 cut(s) 924, 934
DdeI CTNAG 1 cut(s) 879
DpnI GATC 5 cut(s) 56, 417, 675, 873, 1035
DpnII GATC 5 cut(s) 54, 415, 673, 871, 1033
DrdI GACNNNNNNGTC 1 cut(s) 500
DseDI GACNNNNNNGTC 1 cut(s) 500
EaeI YGGCCR 2 cut(s) 111, 465
Eam1104I CTCTTC 3 cut(s) 38, 596, 1053
EarI CTCTTC 3 cut(s) 38, 596, 1053
EciI GGCGGA 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 843
Eco57I CTGAAG 4 cut(s) 39, 62, 481, 597
Eco88I CYCGRG 1 cut(s) 737
EcoRI GAATTC 1 cut(s) 122
EcoT22I ATGCAT 1 cut(s) 1014
FaeI CATG 4 cut(s) 299, 364, 843, 1042
FaqI GGGAC 2 cut(s) 405, 631
FatI CATG 4 cut(s) 295, 360, 839, 1038
FauI CCCGC 1 cut(s) 430
Fnu4HI GCNGC 2 cut(s) 233, 1080
FokI GGATG 2 cut(s) 239, 662
Fsp4HI GCNGC 2 cut(s) 233, 1080
FspBI CTAG 4 cut(s) 218, 677, 921, 950
GlaI GCGC 1 cut(s) 231
GluI GCNGC 2 cut(s) 233, 1080
GsaI CCCAGC 1 cut(s) 413
HaeIII GGCC 3 cut(s) 113, 291, 467
HapII CCGG 1 cut(s) 284
HhaI GCGC 1 cut(s) 232
Hin1II CATG 4 cut(s) 299, 364, 843, 1042
Hin6I GCGC 1 cut(s) 230
HinP1I GCGC 1 cut(s) 230
HinfI GANTC 6 cut(s) 36, 200, 501, 619, 836, 1063
HpaII CCGG 1 cut(s) 284
HphI GGTGA 3 cut(s) 67, 532, 823
Hpy166II GTNNAC 1 cut(s) 936
Hpy188I TCNGA 5 cut(s) 205, 500, 749, 871, 882
Hpy188III TCNNGA 3 cut(s) 40, 370, 716
Hpy8I GTNNAC 1 cut(s) 936
HpyAV CCTTC 1 cut(s) 517
HpyCH4III ACNGT 2 cut(s) 309, 755
HpyCH4IV ACGT 1 cut(s) 24
HpyCH4V TGCA 7 cut(s) 52, 210, 318, 538, 764, 776, 1012
HpyF10VI GCNNNNNNNGC 3 cut(s) 78, 238, 1009
HpyF3I CTNAG 1 cut(s) 879
HpySE526I ACGT 1 cut(s) 24
Hsp92II CATG 4 cut(s) 299, 364, 843, 1042
HspAI GCGC 1 cut(s) 230
KspI CCGCGG 1 cut(s) 236
Kzo9I GATC 5 cut(s) 54, 415, 673, 871, 1033
LmnI GCTCC 3 cut(s) 183, 317, 800
Lsp1109I GCAGC 1 cut(s) 1066
LweI GCATC 4 cut(s) 217, 525, 669, 1021
MaeI CTAG 4 cut(s) 218, 677, 921, 950
MaeII ACGT 1 cut(s) 24
MaeIII GTNAC 1 cut(s) 170
MalI GATC 5 cut(s) 56, 417, 675, 873, 1035
MboI GATC 5 cut(s) 54, 415, 673, 871, 1033
MboII GAAGA 8 cut(s) 32, 55, 249, 362, 382, 535, 583, 1070
MflI RGATCY 1 cut(s) 1033
MhlI GDGCHC 2 cut(s) 272, 314
MluCI AATT 7 cut(s) 122, 137, 253, 386, 398, 779, 970
MlyI GAGTC 4 cut(s) 510, 613, 830, 1057
MnlI CCTC 9 cut(s) 39, 160, 191, 511, 597, 607, 625, 679, 888
Mph1103I ATGCAT 1 cut(s) 1014
MseI TTAA 5 cut(s) 729, 855, 860, 969, 1087
MslI CAYNNNNRTG 2 cut(s) 307, 1043
MspA1I CMGCKG 2 cut(s) 235, 413
MspI CCGG 1 cut(s) 284
MspR9I CCNGG 1 cut(s) 285
Mva1269I GAATGC 1 cut(s) 212
MvnI CGCG 1 cut(s) 235
MwoI GCNNNNNNNGC 3 cut(s) 78, 238, 1009
NciI CCSGG 1 cut(s) 285
NdeII GATC 5 cut(s) 54, 415, 673, 871, 1033
NlaIII CATG 4 cut(s) 299, 364, 843, 1042
NlaIV GGNNCC 5 cut(s) 179, 269, 290, 374, 796
NmuCI GTSAC 1 cut(s) 170
NsiI ATGCAT 1 cut(s) 1014
NspI RCATGY 1 cut(s) 364
NspV TTCGAA 2 cut(s) 34, 86
OliI CACNNNNGTG 1 cut(s) 307
PaqCI CACCTGC 1 cut(s) 86
PctI GAATGC 1 cut(s) 212
PfeI GAWTC 2 cut(s) 36, 200
PflMI CCANNNNNTGG 1 cut(s) 564
PkrI GCNGC 2 cut(s) 234, 1081
PleI GAGTC 4 cut(s) 509, 613, 830, 1057
PpsI GAGTC 4 cut(s) 509, 613, 830, 1057
PsiI TTATAA 1 cut(s) 345
PspFI CCCAGC 1 cut(s) 409
PspN4I GGNNCC 5 cut(s) 179, 269, 290, 374, 796
PspPI GGNCC 2 cut(s) 289, 843
PstNI CAGNNNCTG 1 cut(s) 908
PsuI RGATCY 1 cut(s) 1033
PvuII CAGCTG 1 cut(s) 413
RsaI GTAC 2 cut(s) 925, 935
RsaNI GTAC 2 cut(s) 924, 934
RseI CAYNNNNRTG 2 cut(s) 307, 1043
SacII CCGCGG 1 cut(s) 236
SaqAI TTAA 5 cut(s) 729, 855, 860, 969, 1087
SatI GCNGC 2 cut(s) 233, 1080
Sau3AI GATC 5 cut(s) 54, 415, 673, 871, 1033
Sau96I GGNCC 2 cut(s) 289, 843
SchI GAGTC 4 cut(s) 510, 613, 830, 1057
ScrFI CCNGG 1 cut(s) 285
SduI GDGCHC 2 cut(s) 272, 314
SfaNI GCATC 4 cut(s) 217, 525, 669, 1021
Sfr303I CCGCGG 1 cut(s) 236
SfuI TTCGAA 2 cut(s) 34, 86
SgrBI CCGCGG 1 cut(s) 236
SinI GGWCC 1 cut(s) 843
SmiMI CAYNNNNRTG 2 cut(s) 307, 1043
SpeI ACTAGT 1 cut(s) 920
Sse9I AATT 7 cut(s) 122, 137, 253, 386, 398, 779, 970
SsiI CCGC 6 cut(s) 5, 70, 192, 233, 235, 423
SspMI CTAG 4 cut(s) 218, 677, 921, 950
StyD4I CCNGG 1 cut(s) 283
TaaI ACNGT 2 cut(s) 309, 755
TaiI ACGT 1 cut(s) 27
TaqI TCGA 4 cut(s) 34, 86, 594, 1050
TasI AATT 7 cut(s) 122, 137, 253, 386, 398, 779, 970
TatI WGTACW 2 cut(s) 923, 933
TauI GCSGC 1 cut(s) 235
TfiI GAWTC 2 cut(s) 36, 200
Tru1I TTAA 5 cut(s) 729, 855, 860, 969, 1087
Tru9I TTAA 5 cut(s) 729, 855, 860, 969, 1087
TscAI CASTG 2 cut(s) 475, 711
TseFI GTSAC 1 cut(s) 170
TseI GCWGC 1 cut(s) 1079
Tsp45I GTSAC 1 cut(s) 170
TspDTI ATGAA 4 cut(s) 629, 666, 830, 1071
TspRI CASTG 2 cut(s) 475, 711
Van91I CCANNNNNTGG 1 cut(s) 564
VpaK11BI GGWCC 1 cut(s) 843
XapI RAATTY 2 cut(s) 122, 779
XceI RCATGY 1 cut(s) 364
XspI CTAG 4 cut(s) 218, 677, 921, 950
Zsp2I ATGCAT 1 cut(s) 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.