Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0004735

Physical Location & Seq

Genomic Coordinates Reverse (-)

18286222 .. 18291848

Gene Structure 5627 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000017562

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4503 bp

ATGACCCTGTACATGCCCTTATTCAGGTCGTGCACAACCCTACGAACGCTGACCCAACTCCACGCCCATCTCCTAGTCTCGGGTCTCCACAGACACCCACAAGCCTCCACCAAGCTCATCGAGTCCTACTCCCAACTGGGTTCCCTCCACTCCTCAACCCAGCTCTTTCAAACCTTCCCTAACCAAGACCCTTTCATGTGGGGCGTCCTCATCAAATGCTTCATGTGGAACCGCCGCTTCCACGAAGCCATCTCACTCTACCAAGAAATGCTGCAATGCTCAGTGGGCATGTGTAGTTTTATATTCCCTTCTGTTTTGAGAGCCTGTTCGGGCTGTGGTGAGCTCTGTGTGGGTAGGAAGGTCCATGGGAGGATAGTGAAATGTGGGTTCGACTCGGATGCTGTGGTCGAGACCGCATTGCTTGGTTTGTATGGGGAGCTGGGGTGTTGGGATGATGCAAGGAAGGTGTTTGTTGAAATGCATTGGAGAGACGCCGTGTCGTGGAGTTCGGTTATCTTGTGTTGTGTGGAGAATGGAGAGGTGAGTGAAGGGTTGGAGATGTTCCGTGGGATGGTAATGGAACAAGGTGTTGCACCGGATTCGGTCACAATGCTCAGTGTAGCTGAGGCTTGTGGGAAGTTGGGGTGGCGGAGAGGAGCTAGGTCAGTACATGGTTATGTTGTTAGAAGGGAGTTTAGAAAGGACGAGTCTTTGGATAGTTCTCTGATTACAATGTACAGCAAGTGTGGCGACTTGCAGAGTCCGGAAAGGGTTTTTCGTAATCTTAATGTTGCTCATCGGCACACTGCATCTTGGACCGCGATGATTTCCTGCTACAATCAAGCTGGTTCTTTCTCAGATGCACTAGGTGTTTTCGTTAAGATGCAGGAGTCTGGAGTGGAGCCGAATGCTGTGACGTTCATGTGCATTCTAATGTCTTGTGTTGGGTTAGGCTTGCTCAAAGAAGGGAAGTCTGTTCATGGATTTGTTATTAGAAAGGCTGTAGACCCTGACCTTGATTTTTTGGGGTCAGCATTGTTAGAGTTATATGCTGAAATTGGGGGACTAAGCTATAGTCAGAAAATTCTTAATATGATTGGAGTCAGAAATGTTGTTCCATGGAATACAATTATTTCAGTTTATTGTCAGAAAGGTCTGCTGAGAGAGGCATTGCTACTCTTTGTGCAGATGCAGGCCCAAGGACTAATGCCAGATTCATTCGGCATGTCAAGTGCTCTCGCCGCTTGTGGAAAGATGGGTTTATTAGATATTGGGCATCAGATACATGGTCATATAGTCAAACGAGGCTATTTGGATGAGTTTGTGTTGAACTCACTAATTGATATGTACTCAAGATGTGGGTTTGTAGATTCAGCATACATAATATTTAACGAGATGAAACATCCTGGTATCATAACATGGAATTCTATGATCTCGGGATTTTCTCGTAGTGGTAACTCTGTCATGGCTATCAGTCTTTTTGACCGAATTTACCTGAACCGTCTTGAGATTGATGAAGTCACCATTTTAAGTGTTATTCAAGCTTGCTCCGAATTAGGTTATTTAGAAAAGGGAAAATGGGTTCACCACAAGCTCATAACCTATGGTGTGAGGAAAGACCTCTATATTGATACAGCTCTAACTGACATGTATGCTAAGTGTGGTGACCTTCAGTCAGCTCAAGGTGTATTTGATACGATGGTAGAAAGAAGCGTTGTGTCTTGGAGTGTCATGATCGCTGGGTATGGTATACATGGCAAGATATGTGCTGCCATATCACTCTTTAGTCAAATGTTACAAACTGGAATCGAACCAAATGAGGTTACATTCATGAATATTCTTTCAGCTTGTAGTCATGCAGGTGCTGTTGAAGAAGGGAGGTTATATTTTAGATCAATGAGGGATTTCGGCATTGAGCCCAATGCAGAACATTTTGCATGTATAGTTGACCTTCTAAGTCGAGCCGGTGATTTGAATGGAGCCTATGATATCATAAAATCAATACCATTCTCTGTTGATGCCAGCATATGGGGCGCCCTGCTCAACGGATGTCGAATCCACCAGAGGATGGACATGATTAAAAGCATCGAAAAAGACCTTTTATATATTAAGACAGATGACACTGGCTACTACACCTTATTTTCTAACATATATGCTGAAGGAGGGAACTGGGAGGAATTTGGGAATGTGAGATTAATGATGAAAGGTATTGGTCTGAGGAAGGTCCCTGGATACAGTGTAATTGAGATTGATAAGCGAGTGTATAGATTCGGAGCCGGAGATACTCCTCTTCCTCAAATGAAGGAGTATATTACAAGTCTGAGTGTGAAGCTGCAATACCATTTCAAACACAACACTTGCGTGTACCTTAATTCTTGCAGAAAGCATGAGGTAGAGCCCAATTCAGCAGTTCTCTCATGGTTTACTCAGGCCAAAGTTCAAAGTTCTCAGAATGAGAAGTGTAGCATAACAATTTCATTAGACCAGCTAAAGGATGCTGACATTTTGCCACTAAAGGACGTGCTTATGTCAAGTGACTCATCTCATATTCATGCAGTTGACTTATTTCTGGAATTCCATACTGACTTGGGCACAGAAAGTGTTGTGGATTTGATGAATGGACTCAGTGCAAAGCTTCGAGTTGTCGATCTCCAGGATTTGTCTTTGGGGAAAGAATTTCTGCGGGATCTTTATCAGGGTTCTTTGGCTTGCCAAGTCTTCAACTTGCGGTCCATTCATATCCAGAAACTTGACATGGTTGGAAACTTTTTGCTACTACACACCCTTAATCTCGACTTTTGTACTTCACTCGGTAGTTTGCGAAGGGACTGTTTTTCTTGCATGCCAAATCTGATGCGCCTCTCAATGTGTGAGACAAGAATTTCCAATCTCCTGACCACGACTGGTGCTCTGTCAAGACTTCCTTGTTTGCTAGAACTACGGTTCCAAAGTTGTTTATGTTGCAAGGAAACTGTATCATGTCCTGGTTTAGAAGGGCGACAGAAATTGCAGACTGAGACTCAAGAAAAAGATGAAACCCATGTAAGTGAGGGTAAGAAAGCCTCGAGGAATGTGAGTATTACATTGAAGAACTTTGTAACTCATCATCCTTCACCCATATGCTTTGAGAAACATTATCGGGAGTATATCATATCTTTGCTGCCTGGTCTGGAAGTTTTAGATAATTTTCCTATTAGAATGACGGAGAGGGAATTGGCCAAGATGACGTCTGCAAAGTACTATGAATACTTACCTTATAAAAGACGGCACAAAGAAAGTGTTGTTAGTGTTTTGAATAAGCGTGAAATGGGAACTAGTGGTGTACACTTCCAGAAACCATTCAAGCCAAAACAATTATATCCTCATCGAAATGGGCAGAATTTTTTCTTGAGGTCGCTGTGTGCTGCCAAACTTGGGTCTTCAACTTGGCCACTGTTACATCCACTATCCAATTTCAACCACACATGTAAAGCAGAAAACAAGAAGCTTCGTCCAAGGCAGTTTGAGTATCATCCATCAAACCCAAGTCTTATGGTTTTCGGAACTCTGGATGGTGAAGTAGTGGTTACTAACCATGAAAACGGGAAGATGGTTGGTTATATTCCATGCATAGGAGCAATGAGTAGTGTCTTGGGGCTTTGTTGGCTCAACAAGTCTCCGTCCAAGCTTGTTGCTGGTTCAGATAAGGGCTCCTTGAAGTTACTCAACATCAACAACATGTCACCAGAAGTTGCAGACTCTTGTTGCAGCTCTAGTGGTGCAACTTTTGAAGATTTTGAGCAATTGACTTCTCTTCATGTAAATTCAACTGATGATCAGCTTCTCGCTAGTGGATACTCCAAAGGCGTTGCTCTGTATGACGTTGGCAGTGGAAAACGTGTAGAGTTGTTCAGTAATCTTCACCGAGAACCGATCAATGTTGCCAAATTTGCACACCATTCCCCTTATATGTTTGCTACTTCATCCTTTGACCATGATGTGAAGATGTGGGATTTGAGACAGAGCCTAAGGAAACCGTGCTATACAGCTTCTAGCTCGAGAGGAAATGTGATGGCTTGCTTTTCTCCTGATGACCATTATTTGCTTGTTTCAGCTGTTGACAACGAGGTTAAGCAACTTTTGGCGGCAGATGGGAGGCTGCACACGAGTTTTGAGATAGCTTCAACAGGAAGTCCTCATAACTATACGCGGTCGTATTACATGAATGGAAGGGACTATATCATCAGTGGAAGTTGCGATGAGGACGTGATTCGCATCTGCTGTGCTCAAACTGGAAAGCGACTTAGGGATGTTTATTTGGAGGATGAATCAGGAAATTCAATATTCGTGCAGTCCTTAAGGGGCGATCCTTTCAGGCATTTTAACATGAGTATATTAGCCACTGCTACACGCCCAAGCTTCAAGTGGGAGATTATCAAGGTCAACTTACTTGCATCGAGTGACTCTGCAGAAGAACATTGTTATGGCCAGTGTTTGCAGCCTTCTTGCTGTCTGAGAGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000209 GO:0000715 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006289 GO:0006293 GO:0006294 GO:0006296 GO:0006325 GO:0006464 GO:0006508 GO:0006513 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009987 GO:0010390 GO:0016043 GO:0016567 GO:0016569 GO:0016570 GO:0016574 GO:0016579 GO:0019538 GO:0022607 GO:0030054 GO:0031334 GO:0031461 GO:0031465 GO:0031974 GO:0031981 GO:0032446 GO:0032991 GO:0033522 GO:0033554 GO:0033683 GO:0034622 GO:0034641 GO:0034644 GO:0035518 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043412 GO:0043687 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044877 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051276 GO:0051716 GO:0051865 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070646 GO:0070647 GO:0070911 GO:0070914 GO:0071214 GO:0071478 GO:0071482 GO:0071704 GO:0071824 GO:0071840 GO:0080008 GO:0090304 GO:0090305 GO:0097159 GO:0104004 GO:1901360 GO:1901363 GO:1901564 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

1501

Amino Acids

168.05

Weight (kDa)

7.05

Isoelectric Point (pI)

44.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	269 - 313	6.5e-08	PPR repeat family
PPR	PF01535	271 - 300	3e-06	PPR repeat
PPR_2	PF13041	369 - 416	9.1e-09	PPR repeat family
PPR_1	PF12854	369 - 398	3.5e-06	PPR repeat
PPR	PF01535	373 - 401	1.7e-06	PPR repeat
PPR_2	PF13041	469 - 517	1.8e-06	PPR repeat family
PPR_2	PF13041	571 - 618	4.3e-10	PPR repeat family
E_motif	PF20431	699 - 749	1.9e-10	E motif
DHU1_N	PF20919	789 - 991	2.8e-46	DWD hypersensitive to UV-B 1, N-terminal
DHU1_N	PF20919	1008 - 1075	5.6e-08	DWD hypersensitive to UV-B 1, N-terminal
WD40_Prp19	PF24814	1168 - 1371	1.1e-09	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	1176 - 1345	1.8e-06	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	1181 - 1417	2.9e-15	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	1182 - 1371	5e-09	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	1182 - 1373	6e-09	WDR36/Utp21 first beta-propeller
Beta-prop_THOC3	PF25174	1262 - 1430	6.2e-11	THOC3 beta-propeller domain
WDR55	PF24796	1268 - 1371	9.8e-06	WDR55

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0004735)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69350
fragaria_vesca	FvH4_1g17060 FvH4_1g17060 FvH4_1g17060 FvH4_1g17060 FvH4_1g17060 FvH4_1g17060
malus_domestica	MD02G1180800.v1.1 MD16G1065000.v1.1
prunus_persica	Prupe.1G281900_v2.0.a1 Prupe.6G225400_v2.0.a1 Prupe.6G225400_v2.0.a1 Prupe.6G225400_v2.0.a1 Prupe.6G225400_v2.0.a1
pyrus_communis	pycom16g05680
rosa_chinensis	RchiOBHm_Chr2g0107231
rosa_laevigata	RLG00000017562
rosa_multiflora	Rmu_co8473113.1_g000001 Rmu_sc0027417.1_g000002 Rmu_sc0034517.1_g000001 Rmu_ssc0000269.1_g000007
rosa_roxburghii	Rroxscaffold_2G00136510 Rroxscaffold_2G00136520
rosa_rugosa	Rorug02G0141100 Rorug02G0141200
rosa_samantha	Rh2AG191900 Rh2BG203800 Rh2CG197100 Rh2DG199200
rosa_wichuraiana	Rw2G015170 Rw2G015180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3258
AarI	CACCTGC	1 cut(s)	1850
AatII	GACGTC	1 cut(s)	3230
AbsI	CCTCGAGG	1 cut(s)	3064
Acc36I	ACCTGC	1 cut(s)	1850
AccB1I	GGYRCC	1 cut(s)	2033
AccB7I	CCANNNNNTGG	2 cut(s)	2028, 2068
AccI	GTMKAC	2 cut(s)	1005, 1750
AccII	CGCG	2 cut(s)	821, 4189
AccIII	TCCGGA	1 cut(s)	763
AclWI	GGATC	2 cut(s)	2694, 4340
AcoI	YGGCCR	3 cut(s)	3216, 3428, 4465
AcuI	CTGAAG	2 cut(s)	1655, 2178
AcyI	GRCGYC	4 cut(s)	204, 492, 2034, 3227
AdeI	CACNNNGTG	1 cut(s)	869
AfaI	GTAC	8 cut(s)	11, 669, 737, 1349, 2366, 2803, 3239, 3324
AfiI	CCNNNNNNNGG	9 cut(s)	24, 79, 501, 571, 2028, 2068, 2491, 3414, 3611
AflII	CTTAAG	1 cut(s)	4336
AflIII	ACRYGT	4 cut(s)	1647, 3464, 3717, 3877
AhdI	GACNNNNNGTC	2 cut(s)	496, 1672
AhlI	ACTAGT	1 cut(s)	3314
AjiI	CACGTC	2 cut(s)	2521, 4246
AjnI	CCWGG	5 cut(s)	1405, 2227, 2652, 2983, 3163
AleI	CACNNNNGTG	1 cut(s)	2360
AloI	GAACNNNNNNTCC	4 cut(s)	1566, 1598, 2928, 2960
Alw21I	GWGCWC	5 cut(s)	35, 345, 1237, 2911, 4267
Alw26I	GTCTC	8 cut(s)	82, 89, 404, 483, 2867, 3011, 3660, 3991
Alw44I	GTGCAC	1 cut(s)	31
AlwI	GGATC	2 cut(s)	2694, 4340
AlwNI	CAGNNNCTG	1 cut(s)	1865
Ama87I	CYCGRG	4 cut(s)	79, 1435, 3064, 4036
Aor13HI	TCCGGA	1 cut(s)	763
AoxI	GGCC	5 cut(s)	1194, 2430, 3216, 3428, 4465
ApaLI	GTGCAC	1 cut(s)	31
ApeKI	GCWGC	8 cut(s)	271, 1769, 2332, 3160, 3404, 3747, 4138, 4477
AseI	ATTAAT	1 cut(s)	2195
Asp700I	GAANNNNTTC	2 cut(s)	3338, 3383
AspLEI	GCGC	2 cut(s)	2036, 2859
AspS9I	GGNCC	5 cut(s)	361, 816, 1195, 2224, 2730
AvaI	CYCGRG	4 cut(s)	79, 1435, 3064, 4036
AvaII	GGWCC	4 cut(s)	361, 816, 2224, 2730
AxyI	CCTNAGG	1 cut(s)	4007
BaeGI	GKGCMC	2 cut(s)	35, 2594
BaeI	ACNNNNGTAYC	2 cut(s)	1686, 1719
BalI	TGGCCA	3 cut(s)	3218, 3430, 4467
BanI	GGYRCC	1 cut(s)	2033
BanII	GRGCYC	4 cut(s)	345, 1920, 2400, 3692
BarI	GAAGNNNNNNTAC	2 cut(s)	2321, 2353
BauI	CACGAG	1 cut(s)	4144
BbsI	GAAGAC	2 cut(s)	2710, 3411
Bbv12I	GWGCWC	5 cut(s)	35, 345, 1237, 2911, 4267
BbvCI	CCTCAGC	1 cut(s)	624
BbvI	GCAGC	8 cut(s)	258, 1756, 2319, 3147, 3391, 3759, 4125, 4489
BceAI	ACGGC	2 cut(s)	479, 3281
BcgI	CGANNNNNNTGC	4 cut(s)	380, 414, 582, 616
BciT130I	CCWGG	5 cut(s)	1407, 2229, 2654, 2985, 3165
BciVI	GTATCC	2 cut(s)	2225, 3827
BclI	TGATCA	1 cut(s)	3814
BcoDI	GTCTC	8 cut(s)	82, 89, 404, 483, 2867, 3011, 3660, 3991
BcuI	ACTAGT	1 cut(s)	3314
BfaI	CTAG	8 cut(s)	74, 660, 866, 2933, 3315, 3753, 3828, 4032
BfmI	CTRYAG	3 cut(s)	1002, 1072, 4446
BfoI	RGCGCY	1 cut(s)	2037
BfrI	CTTAAG	1 cut(s)	4336
BfuAI	ACCTGC	1 cut(s)	1850
BfuI	GTATCC	2 cut(s)	2225, 3827
BmcAI	AGTACT	1 cut(s)	3239
Bme1390I	CCNGG	5 cut(s)	1407, 2229, 2654, 2985, 3165
Bme18I	GGWCC	4 cut(s)	361, 816, 2224, 2730
BmeRI	GACNNNNNGTC	2 cut(s)	496, 1672
BmeT110I	CYCGRG	4 cut(s)	79, 1435, 3064, 4036
BmgBI	CACGTC	2 cut(s)	2521, 4246
BmgT120I	GGNCC	5 cut(s)	361, 816, 1195, 2224, 2730
BmiI	GGNNCC	9 cut(s)	142, 230, 903, 1981, 2035, 2226, 2275, 2945, 3691
BmrFI	CCNGG	5 cut(s)	1407, 2229, 2654, 2985, 3165
BmrI	ACTGGG	2 cut(s)	146, 2179
BmuI	ACTGGG	2 cut(s)	146, 2179
BpiI	GAAGAC	2 cut(s)	2710, 3411
BplI	GAGNNNNNCTC	2 cut(s)	113, 145
BpmI	CTGGAG	2 cut(s)	915, 2636
Bpu10I	CCTNAGC	1 cut(s)	624
BpuEI	CTTGAG	5 cut(s)	1336, 1526, 1665, 3006, 3409
BsaBI	GATNNNNATC	2 cut(s)	2691, 4253
BsaHI	GRCGYC	4 cut(s)	204, 492, 2034, 3227
BsaI	GGTCTC	2 cut(s)	89, 404
BsaJI	CCNNGG	6 cut(s)	364, 565, 1118, 1198, 2227, 3494
BsaWI	WCCGGW	2 cut(s)	595, 763
BsaXI	ACNNNNNCTCC	2 cut(s)	428, 458
Bsc4I	CCNNNNNNNGG	9 cut(s)	24, 79, 501, 571, 2028, 2068, 2491, 3414, 3611
Bse118I	RCCGGY	1 cut(s)	1964
Bse1I	ACTGG	7 cut(s)	141, 1807, 2128, 2174, 2908, 4276, 4468
Bse21I	CCTNAGG	1 cut(s)	4007
Bse3DI	GCAATG	4 cut(s)	281, 416, 1169, 3624
Bse8I	GATNNNNATC	2 cut(s)	2691, 4253
BseAI	TCCGGA	1 cut(s)	763
BseBI	CCWGG	5 cut(s)	1407, 2229, 2654, 2985, 3165
BseDI	CCNNGG	6 cut(s)	364, 565, 1118, 1198, 2227, 3494
BseJI	GATNNNNATC	2 cut(s)	2691, 4253
BseLI	CCNNNNNNNGG	9 cut(s)	24, 79, 501, 571, 2028, 2068, 2491, 3414, 3611
BseMI	GCAATG	4 cut(s)	281, 416, 1169, 3624
BseNI	ACTGG	7 cut(s)	141, 1807, 2128, 2174, 2908, 4276, 4468
BseRI	GAGGAG	3 cut(s)	142, 669, 2277
BseSI	GKGCMC	2 cut(s)	35, 2594
BseXI	GCAGC	8 cut(s)	258, 1756, 2319, 3147, 3391, 3759, 4125, 4489
BseYI	CCCAGC	3 cut(s)	159, 439, 1739
BsgI	GTGCAG	3 cut(s)	1205, 4124, 4349
Bsh1236I	CGCG	2 cut(s)	821, 4189
Bsh1285I	CGRYCG	1 cut(s)	4193
BshFI	GGCC	5 cut(s)	1196, 2432, 3218, 3430, 4467
BshNI	GGYRCC	1 cut(s)	2033
BsiEI	CGRYCG	1 cut(s)	4193
BsiHKAI	GWGCWC	5 cut(s)	35, 345, 1237, 2911, 4267
BsiHKCI	CYCGRG	4 cut(s)	79, 1435, 3064, 4036
BsiSI	CCGG	4 cut(s)	596, 764, 1965, 2277
BslFI	GGGAC	4 cut(s)	1077, 2210, 2840, 4226
BslI	CCNNNNNNNGG	9 cut(s)	24, 79, 501, 571, 2028, 2068, 2491, 3414, 3611
BsmAI	GTCTC	8 cut(s)	82, 89, 404, 483, 2867, 3011, 3660, 3991
BsmBI	CGTCTC	1 cut(s)	483
BsmFI	GGGAC	4 cut(s)	1077, 2210, 2840, 4226
BsmI	GAATGC	2 cut(s)	913, 927
BsnI	GGCC	5 cut(s)	1196, 2432, 3218, 3430, 4467
Bso31I	GGTCTC	2 cut(s)	89, 404
BsoBI	CYCGRG	4 cut(s)	79, 1435, 3064, 4036
Bsp1286I	GDGCHC	9 cut(s)	35, 345, 1237, 1920, 2400, 2594, 2911, 3692, 4267
Bsp13I	TCCGGA	1 cut(s)	763
Bsp1407I	TGTACA	3 cut(s)	9, 735, 3322
Bsp143I	GATC	8 cut(s)	1431, 1734, 1892, 2647, 2686, 3814, 3912, 4345
Bsp19I	CCATGG	2 cut(s)	364, 1118
BspANI	GGCC	5 cut(s)	1196, 2432, 3218, 3430, 4467
BspEI	TCCGGA	1 cut(s)	763
BspFNI	CGCG	2 cut(s)	821, 4189
BspHI	TCATGA	2 cut(s)	1731, 1830
BspLI	GGNNCC	9 cut(s)	142, 230, 903, 1981, 2035, 2226, 2275, 2945, 3691
BspMAI	CTGCAG	1 cut(s)	4450
BspMI	ACCTGC	1 cut(s)	1850
BspPI	GGATC	2 cut(s)	2694, 4340
BspT107I	GGYRCC	1 cut(s)	2033
BspTI	CTTAAG	1 cut(s)	4336
BspTNI	GGTCTC	2 cut(s)	89, 404
BsrDI	GCAATG	4 cut(s)	281, 416, 1169, 3624
BsrFI	RCCGGY	1 cut(s)	1964
BsrGI	TGTACA	3 cut(s)	9, 735, 3322
BsrI	ACTGG	7 cut(s)	141, 1807, 2128, 2174, 2908, 4276, 4468
BssAI	RCCGGY	1 cut(s)	1964
BssECI	CCNNGG	6 cut(s)	364, 565, 1118, 1198, 2227, 3494
BssMI	GATC	8 cut(s)	1431, 1734, 1892, 2647, 2686, 3814, 3912, 4345
BssNAI	GTATAC	1 cut(s)	1751
BssNI	GRCGYC	4 cut(s)	204, 492, 2034, 3227
BssSI	CACGAG	1 cut(s)	4144
BssT1I	CCWWGG	4 cut(s)	364, 1118, 1198, 3494
Bst1107I	GTATAC	1 cut(s)	1751
Bst2BI	CACGAG	1 cut(s)	4144
Bst2UI	CCWGG	5 cut(s)	1407, 2229, 2654, 2985, 3165
Bst4CI	ACNGT	7 cut(s)	1502, 2237, 2831, 2943, 2974, 3435, 4017
Bst6I	CTCTTC	2 cut(s)	2295, 3798
BstACI	GRCGYC	4 cut(s)	204, 492, 2034, 3227
BstAFI	CTTAAG	1 cut(s)	4336
BstAUI	TGTACA	3 cut(s)	9, 735, 3322
BstC8I	GCNNGC	7 cut(s)	956, 1194, 1546, 2023, 2710, 2843, 4057
BstDSI	CCRYGG	3 cut(s)	364, 565, 1118
BstEII	GGTNACC	1 cut(s)	1664
BstENI	CCTNNNNNAGG	1 cut(s)	22
BstFNI	CGCG	2 cut(s)	821, 4189
BstH2I	RGCGCY	1 cut(s)	2037
BstHHI	GCGC	2 cut(s)	2036, 2859
BstKTI	GATC	8 cut(s)	1434, 1737, 1895, 2650, 2689, 3817, 3915, 4348
BstMAI	GTCTC	8 cut(s)	82, 89, 404, 483, 2867, 3011, 3660, 3991
BstMBI	GATC	8 cut(s)	1431, 1734, 1892, 2647, 2686, 3814, 3912, 4345
BstMCI	CGRYCG	1 cut(s)	4193
BstMWI	GCNNNNNNNGC	6 cut(s)	285, 747, 1241, 2031, 3643, 3929
BstNI	CCWGG	5 cut(s)	1407, 2229, 2654, 2985, 3165
BstNSI	RCATGY	8 cut(s)	16, 292, 1228, 1651, 1941, 2845, 3468, 3721
BstPI	GGTNACC	1 cut(s)	1664
BstSCI	CCNGG	5 cut(s)	1405, 2227, 2652, 2983, 3163
BstSFI	CTRYAG	3 cut(s)	1002, 1072, 4446
BstSLI	GKGCMC	2 cut(s)	35, 2594
BstUI	CGCG	2 cut(s)	821, 4189
BstV1I	GCAGC	8 cut(s)	258, 1756, 2319, 3147, 3391, 3759, 4125, 4489
BstV2I	GAAGAC	2 cut(s)	2710, 3411
BstX2I	RGATCY	1 cut(s)	2686
BstYI	RGATCY	1 cut(s)	2686
BstZ17I	GTATAC	1 cut(s)	1751
Bsu36I	CCTNAGG	1 cut(s)	4007
BsuI	GTATCC	2 cut(s)	2225, 3827
BsuRI	GGCC	5 cut(s)	1196, 2432, 3218, 3430, 4467
BtgI	CCRYGG	3 cut(s)	364, 565, 1118
BtgZI	GCGATG	2 cut(s)	836, 4251
BtrI	CACGTC	2 cut(s)	2521, 4246
BtsI	GCAGTG	3 cut(s)	804, 3874, 4380
BveI	ACCTGC	1 cut(s)	1850
Cac8I	GCNNGC	7 cut(s)	956, 1194, 1546, 2023, 2710, 2843, 4057
CaiI	CAGNNNCTG	1 cut(s)	1865
CciI	TCATGA	2 cut(s)	1731, 1830
CfoI	GCGC	2 cut(s)	2036, 2859
Cfr10I	RCCGGY	1 cut(s)	1964
Cfr13I	GGNCC	5 cut(s)	361, 816, 1195, 2224, 2730
CseI	GACGC	2 cut(s)	193, 500
Csp6I	GTAC	8 cut(s)	10, 668, 736, 1348, 2365, 2802, 3238, 3323
CviQI	GTAC	8 cut(s)	10, 668, 736, 1348, 2365, 2802, 3238, 3323
DinI	GGCGCC	1 cut(s)	2035
DpnI	GATC	8 cut(s)	1433, 1736, 1894, 2649, 2688, 3816, 3914, 4347
DpnII	GATC	8 cut(s)	1431, 1734, 1892, 2647, 2686, 3814, 3912, 4345
DraIII	CACNNNGTG	1 cut(s)	869
DriI	GACNNNNNGTC	2 cut(s)	496, 1672
EaeI	YGGCCR	3 cut(s)	3216, 3428, 4465
Eam1104I	CTCTTC	2 cut(s)	2295, 3798
Eam1105I	GACNNNNNGTC	2 cut(s)	496, 1672
EarI	CTCTTC	2 cut(s)	2295, 3798
EciI	GGCGGA	1 cut(s)	664
Ecl136II	GAGCTC	1 cut(s)	343
Eco130I	CCWWGG	4 cut(s)	364, 1118, 1198, 3494
Eco24I	GRGCYC	4 cut(s)	345, 1920, 2400, 3692
Eco31I	GGTCTC	2 cut(s)	89, 404
Eco32I	GATATC	1 cut(s)	1990
Eco47I	GGWCC	4 cut(s)	361, 816, 2224, 2730
Eco53kI	GAGCTC	1 cut(s)	343
Eco57I	CTGAAG	2 cut(s)	1655, 2178
Eco81I	CCTNAGG	1 cut(s)	4007
Eco88I	CYCGRG	4 cut(s)	79, 1435, 3064, 4036
Eco91I	GGTNACC	1 cut(s)	1664
EcoICRI	GAGCTC	1 cut(s)	343
EcoNI	CCTNNNNNAGG	1 cut(s)	22
EcoO109I	RGGNCCY	1 cut(s)	2224
EcoO65I	GGTNACC	1 cut(s)	1664
EcoRI	GAATTC	2 cut(s)	1423, 2573
EcoRII	CCWGG	5 cut(s)	1405, 2227, 2652, 2983, 3163
EcoRV	GATATC	1 cut(s)	1990
EcoT14I	CCWWGG	4 cut(s)	364, 1118, 1198, 3494
EcoT22I	ATGCAT	2 cut(s)	483, 3611
EcoT38I	GRGCYC	4 cut(s)	345, 1920, 2400, 3692
EgeI	GGCGCC	1 cut(s)	2035
EheI	GGCGCC	1 cut(s)	2035
ErhI	CCWWGG	4 cut(s)	364, 1118, 1198, 3494
Esp3I	CGTCTC	1 cut(s)	483
FalI	AAGNNNNNCTT	6 cut(s)	2908, 2940, 3677, 3709, 4409, 4441
FaqI	GGGAC	4 cut(s)	1077, 2210, 2840, 4226
FauI	CCCGC	1 cut(s)	2676
FauNDI	CATATG	2 cut(s)	2027, 3119
FbaI	TGATCA	1 cut(s)	3814
FblI	GTMKAC	2 cut(s)	1005, 1750
FriOI	GRGCYC	4 cut(s)	345, 1920, 2400, 3692
FspBI	CTAG	8 cut(s)	74, 660, 866, 2933, 3315, 3753, 3828, 4032
GlaI	GCGC	2 cut(s)	2035, 2858
GsaI	CCCAGC	3 cut(s)	163, 443, 1743
GsuI	CTGGAG	2 cut(s)	915, 2636
HaeII	RGCGCY	1 cut(s)	2037
HaeIII	GGCC	5 cut(s)	1196, 2432, 3218, 3430, 4467
HapII	CCGG	4 cut(s)	596, 764, 1965, 2277
HgaI	GACGC	2 cut(s)	193, 500
HhaI	GCGC	2 cut(s)	2036, 2859
Hin1I	GRCGYC	4 cut(s)	204, 492, 2034, 3227
Hin6I	GCGC	2 cut(s)	2034, 2857
HinP1I	GCGC	2 cut(s)	2034, 2857
HincII	GTYRAC	4 cut(s)	1948, 2560, 4099, 4423
HindII	GTYRAC	4 cut(s)	1948, 2560, 4099, 4423
HindIII	AAGCTT	5 cut(s)	1542, 2633, 3485, 3665, 4396
HpaII	CCGG	4 cut(s)	596, 764, 1965, 2277
HpyCH4III	ACNGT	7 cut(s)	1502, 2237, 2831, 2943, 2974, 3435, 4017
HpyCH4IV	ACGT	6 cut(s)	917, 2520, 3227, 3861, 3877, 4245
HpyF10VI	GCNNNNNNNGC	6 cut(s)	285, 747, 1241, 2031, 3643, 3929
HpySE526I	ACGT	6 cut(s)	917, 2520, 3227, 3861, 3877, 4245
Hsp92I	GRCGYC	4 cut(s)	204, 492, 2034, 3227
HspAI	GCGC	2 cut(s)	2034, 2857
KasI	GGCGCC	1 cut(s)	2033
Kpn2I	TCCGGA	1 cut(s)	763
Ksp22I	TGATCA	1 cut(s)	3814
Kzo9I	GATC	8 cut(s)	1431, 1734, 1892, 2647, 2686, 3814, 3912, 4345
LmnI	GCTCC	8 cut(s)	436, 656, 901, 1553, 1979, 2273, 3614, 3695
Lsp1109I	GCAGC	8 cut(s)	258, 1756, 2319, 3147, 3391, 3759, 4125, 4489
MaeI	CTAG	8 cut(s)	74, 660, 866, 2933, 3315, 3753, 3828, 4032
MaeII	ACGT	6 cut(s)	917, 2520, 3227, 3861, 3877, 4245
MalI	GATC	8 cut(s)	1433, 1736, 1894, 2649, 2688, 3816, 3914, 4347
MboI	GATC	8 cut(s)	1431, 1734, 1892, 2647, 2686, 3814, 3912, 4345
MfeI	CAATTG	1 cut(s)	3782
MflI	RGATCY	1 cut(s)	2686
MhlI	GDGCHC	9 cut(s)	35, 345, 1237, 1920, 2400, 2594, 2911, 3692, 4267
MlsI	TGGCCA	3 cut(s)	3218, 3430, 4467
MluNI	TGGCCA	3 cut(s)	3218, 3430, 4467
Mly113I	GGCGCC	1 cut(s)	2034
MmeI	TCCRAC	2 cut(s)	534, 2740
Mox20I	TGGCCA	3 cut(s)	3218, 3430, 4467
Mph1103I	ATGCAT	2 cut(s)	483, 3611
MroI	TCCGGA	1 cut(s)	763
MroXI	GAANNNNTTC	2 cut(s)	3338, 3383
MscI	TGGCCA	3 cut(s)	3218, 3430, 4467
MslI	CAYNNNNRTG	9 cut(s)	675, 932, 1860, 2360, 2550, 3045, 3118, 3369, 4461
Msp20I	TGGCCA	3 cut(s)	3218, 3430, 4467
MspA1I	CMGCKG	1 cut(s)	4094
MspCI	CTTAAG	1 cut(s)	4336
MspI	CCGG	4 cut(s)	596, 764, 1965, 2277
MspR9I	CCNGG	5 cut(s)	1407, 2229, 2654, 2985, 3165
MunI	CAATTG	1 cut(s)	3782
Mva1269I	GAATGC	2 cut(s)	913, 927
MvaI	CCWGG	5 cut(s)	1407, 2229, 2654, 2985, 3165
MvnI	CGCG	2 cut(s)	821, 4189
MwoI	GCNNNNNNNGC	6 cut(s)	285, 747, 1241, 2031, 3643, 3929
NarI	GGCGCC	1 cut(s)	2034
NcoI	CCATGG	2 cut(s)	364, 1118
NdeI	CATATG	2 cut(s)	2027, 3119
NdeII	GATC	8 cut(s)	1431, 1734, 1892, 2647, 2686, 3814, 3912, 4345
NlaIV	GGNNCC	9 cut(s)	142, 230, 903, 1981, 2035, 2226, 2275, 2945, 3691
NmuCI	GTSAC	7 cut(s)	604, 913, 1519, 1664, 2534, 3720, 4439
NsiI	ATGCAT	2 cut(s)	483, 3611
NspI	RCATGY	8 cut(s)	16, 292, 1228, 1651, 1941, 2845, 3468, 3721
OliI	CACNNNNGTG	1 cut(s)	2360
PaeI	GCATGC	1 cut(s)	2845
PaeR7I	CTCGAG	2 cut(s)	3064, 4036
PagI	TCATGA	2 cut(s)	1731, 1830
PaqCI	CACCTGC	1 cut(s)	1850
PciI	ACATGT	3 cut(s)	1647, 3464, 3717
PctI	GAATGC	2 cut(s)	913, 927
PdmI	GAANNNNTTC	2 cut(s)	3338, 3383
PfeI	GAWTC	8 cut(s)	599, 1214, 1370, 1806, 2055, 2268, 4249, 4307
PflMI	CCANNNNNTGG	2 cut(s)	2028, 2068
PfoI	TCCNGGA	1 cut(s)	2652
PluTI	GGCGCC	1 cut(s)	2037
PpuMI	RGGWCCY	1 cut(s)	2224
PscI	ACATGT	3 cut(s)	1647, 3464, 3717
PshBI	ATTAAT	1 cut(s)	2195
PsiI	TTATAA	1 cut(s)	3258
Psp124BI	GAGCTC	1 cut(s)	345
Psp5II	RGGWCCY	1 cut(s)	2224
Psp6I	CCWGG	5 cut(s)	1405, 2227, 2652, 2983, 3163
PspEI	GGTNACC	1 cut(s)	1664
PspFI	CCCAGC	3 cut(s)	159, 439, 1739
PspGI	CCWGG	5 cut(s)	1405, 2227, 2652, 2983, 3163
PspN4I	GGNNCC	9 cut(s)	142, 230, 903, 1981, 2035, 2226, 2275, 2945, 3691
PspPI	GGNCC	5 cut(s)	361, 816, 1195, 2224, 2730
PspPPI	RGGWCCY	1 cut(s)	2224
PspXI	VCTCGAGB	1 cut(s)	3064
PstI	CTGCAG	1 cut(s)	4450
PstNI	CAGNNNCTG	1 cut(s)	1865
PsuI	RGATCY	1 cut(s)	2686
PvuII	CAGCTG	1 cut(s)	4094
RsaI	GTAC	8 cut(s)	11, 669, 737, 1349, 2366, 2803, 3239, 3324
RsaNI	GTAC	8 cut(s)	10, 668, 736, 1348, 2365, 2802, 3238, 3323
RseI	CAYNNNNRTG	9 cut(s)	675, 932, 1860, 2360, 2550, 3045, 3118, 3369, 4461
SacI	GAGCTC	1 cut(s)	345
Sau3AI	GATC	8 cut(s)	1431, 1734, 1892, 2647, 2686, 3814, 3912, 4345
Sau96I	GGNCC	5 cut(s)	361, 816, 1195, 2224, 2730
ScaI	AGTACT	1 cut(s)	3239
ScrFI	CCNGG	5 cut(s)	1407, 2229, 2654, 2985, 3165
SduI	GDGCHC	9 cut(s)	35, 345, 1237, 1920, 2400, 2594, 2911, 3692, 4267
SfcI	CTRYAG	3 cut(s)	1002, 1072, 4446
SfoI	GGCGCC	1 cut(s)	2035
Sfr274I	CTCGAG	2 cut(s)	3064, 4036
SinI	GGWCC	4 cut(s)	361, 816, 2224, 2730
SlaI	CTCGAG	2 cut(s)	3064, 4036
SmiMI	CAYNNNNRTG	9 cut(s)	675, 932, 1860, 2360, 2550, 3045, 3118, 3369, 4461
SmlI	CTYRAG	8 cut(s)	1351, 1505, 1680, 3021, 3064, 3388, 4036, 4336
SmoI	CTYRAG	8 cut(s)	1351, 1505, 1680, 3021, 3064, 3388, 4036, 4336
SpeI	ACTAGT	1 cut(s)	3314
SphI	GCATGC	1 cut(s)	2845
SspDI	GGCGCC	1 cut(s)	2033
SspI	AATATT	3 cut(s)	1386, 1837, 4323
SspMI	CTAG	8 cut(s)	74, 660, 866, 2933, 3315, 3753, 3828, 4032
SstI	GAGCTC	1 cut(s)	345
StyD4I	CCNGG	5 cut(s)	1405, 2227, 2652, 2983, 3163
StyI	CCWWGG	4 cut(s)	364, 1118, 1198, 3494
TaaI	ACNGT	7 cut(s)	1502, 2237, 2831, 2943, 2974, 3435, 4017
TaiI	ACGT	6 cut(s)	920, 2523, 3230, 3864, 3880, 4248
TaqII	GACCGA	2 cut(s)	592, 1500
TatI	WGTACW	7 cut(s)	9, 667, 735, 1347, 2801, 3237, 3322
TauI	GCSGC	3 cut(s)	237, 1244, 4127
TfiI	GAWTC	8 cut(s)	599, 1214, 1370, 1806, 2055, 2268, 4249, 4307
TseFI	GTSAC	7 cut(s)	604, 913, 1519, 1664, 2534, 3720, 4439
TseI	GCWGC	8 cut(s)	271, 1769, 2332, 3160, 3404, 3747, 4138, 4477
Tsp45I	GTSAC	7 cut(s)	604, 913, 1519, 1664, 2534, 3720, 4439
TspGWI	ACGGA	4 cut(s)	554, 2061, 3218, 3648
Van91I	CCANNNNNTGG	2 cut(s)	2028, 2068
Vha464I	CTTAAG	1 cut(s)	4336
VneI	GTGCAC	1 cut(s)	31
VpaK11BI	GGWCC	4 cut(s)	361, 816, 2224, 2730
VspI	ATTAAT	1 cut(s)	2195
XagI	CCTNNNNNAGG	1 cut(s)	22
XceI	RCATGY	8 cut(s)	16, 292, 1228, 1651, 1941, 2845, 3468, 3721
XcmI	CCANNNNNNNNNTGG	1 cut(s)	4401
XhoI	CTCGAG	2 cut(s)	3064, 4036
XmiI	GTMKAC	2 cut(s)	1005, 1750
XmnI	GAANNNNTTC	2 cut(s)	3338, 3383
XspI	CTAG	8 cut(s)	74, 660, 866, 2933, 3315, 3753, 3828, 4032
ZraI	GACGTC	1 cut(s)	3228
ZrmI	AGTACT	1 cut(s)	3239
Zsp2I	ATGCAT	2 cut(s)	483, 3611

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.