MD16G1065000.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004735
Physical Location & Seq
Forward (+)
4567063 .. 4569405
2343 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1065000.v1.1.491

Sequence Viewer

Length: 2343 bp
ATGACCCTGTACATGCCATTGTTCAGGTCCTGCACCGTCCCACGAACACTCGCCCAACTCCACGCCCACCTCGTCGTCTCGGGTCTCCACAGAGACCCACAGGCCTCCACCAAGCTCATAGAATCCTACGCCCAAATGGGTTCCCTCCAATCTTCCACCCACGTCTTCAAAACCTTTCCTAACCCAGATTCTTTCATGTGCGGCGTGCTCATGAAGTGCTTCGTGTGGAATCACTGCTTCCAAGAAGCGATTTTTCTCTATCACGAAATGTTGCACCGCGAAAACTGCATGAACAGGTTTATATTTCCTTCTGTTTTGAGAGCTTGTTCGGGGTTTGGTGATCTGGGTGTTGGCGGGAAGGTCCATGGGAGGATAATCAAATCTGGGTTTGACTCCGATGCGGTGGTCGAGACCTCGTTGCTTGGTTTGTATGGAGAACTGGGTAGCTTAGGTGATGCCCGGAAGGTGTTTGACGCAATGCCGGTGAGGGATGTGGTGTCGTGGAGTTCGATTATTTCGTGCTCCGTGGAGAATGGGGAAGCAAGTGAAGGGTTGGATATGTTCCGTTGGATGGTTTTTGAAGGTGTTGAACTGGATTCGGTTACAATGCTTTGTGTGGCTGAGGCTTGTGGGGAGTTGGCATTGTTGAGAGAAGCAAGGTCAGTACATGGCCATGTTGTTAGAAGGGGAATTAAGACTGATGGGTCTTTGGATAATTCTCTCATTTCAATGTACAGTAAATGTGGTGACTTGCAAAGTGCAAAAACGATCTTTCGTAGTGTTACTCATTGGGACACTGCATCTTGGACCGCAATGATATCCTGCTACAATCAAACTGGTTCTTTTTTGGAAGCGCTGGACGCTTTTGTTGAGATGCAAGAGTCTAAGGTGGAACCGAATTCGGTAACCTTGATGTGTGTTCTGCGGTCCTGTATTAGGTTAGGCTTGCACAGAGAAGGGAGTTCTGTTCATTGCTTTGCTATTAGGAATGGTGTAGACCCTGACCTTGATTTTTTAGGATCTGCGTTGTTCGAATTGTACTCTAAAATTGGGGGACTAAGCTATTGCCAGAAAGTTATTAATACAATTGGAGGGAGAAATGTTGTTTCATGGAACACAATCATTTCAGGCCATTGTCAGAAAGGTTTGTTGAGAGAAGCATTGGTACTCTTTGTGCAGATGCAGACCCATGGACTAATGCCAGATTCATTCAGCATGTCAAGTGCTCTCTCGGCGTGTGGAAAGGTCGGGTCTTTAGAGCTTGGACATCAGATACATGGTCATATAATCAAAAGAGGCTATTTGGATGAATTTGTGTTGAATTCACTGATTGACATGTACTCAAAATGTGGGTTTGTAGATTCAGCATACATGATATTCGACAAGATTAAACACCTTGGTCTCGTAACATGGAATGCTATGATTTCCGGATTTTCTCAAAATGGTAACCCTGTCATGGCGATCAGTCTTTTTGATGAAATGTACTTGAACTGTCATGAGATCAATGAAGTCACTATATTAAGTATTATCCAAGCTTGTTCTGAATTAGGTTATCTGGAAAAGGGGAAATGGGTTCACCACAAGCTCATAACCTTGGGCATCAGGAAAGATTTGTTTACTGATACAGCTCTAACTGACATGTATGCCAAGTGTGGTGACCTGCGGTCAGCTCAGGGAGTCTTCGATATGATGGAAGAAAGAAGCGTTGTGTCATGGAGTGTCATGATTGCTGGGTATGGTATGCATGGCCGGATTAATGCTGCCACATCACTCTTTGATCATATGGTGGAAACAGGAGTGCAACCAAATGAGATCATTTTCATGAATATTCTTTCAGCTTGCAGTCACGCAGGAGCTGTCGAAAAGGGAAGGTTCTATTTCAGATCAATGAGGGATTTCGGCGTTGAGCCCACTGCTGAACATTTTGCTTGTATAGTTGACCTTCTAAGTCGAGCTGGTGATCTCAACGGAGCATATGAGATTATCAAATCAATGCCAACCCCTGTAGATGCCAGCATATGGGGCGCTTTGCTTAATGGATGTCGAATCCACCAGAGGATGGACATGATCGAAAGCATTAAAAGAGACGTTTTAGATATCAGCACAGATGACACTGGATACTATACCTTATTTTCTAACATATACGCTGAAGGGGGGAACTGGGATGAATTTGGAAATGTGAGACTAATGATGAAAGGTATCGGTCTAAGGAAGGTCCCCGGATACAGTATAATTGAGCTTGATAAGCAAGTTCACAGATTTGGAGCTGGAGATACCCCTCTTCCCCAAATGAAGGAGGTTTACAGTTTCTTGGAAAAATTTCAGTCTCTCGCTCGTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

781

Amino Acids

86.71

Weight (kDa)

5.92

Isoelectric Point (pI)

37.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 265 - 311 1.4e-07 PPR repeat family
PPR_2 PF13041 366 - 415 1.7e-12 PPR repeat family
PPR_1 PF12854 367 - 395 1.1e-08 PPR repeat
PPR PF01535 369 - 399 9.5e-08 PPR repeat
PPR_2 PF13041 469 - 514 8.8e-09 PPR repeat family
PPR PF01535 469 - 494 3e-08 PPR repeat
PPR_2 PF13041 568 - 604 6.2e-08 PPR repeat family
E_motif PF20431 697 - 746 1.4e-10 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 703
Acc36I ACCTGC 1 cut(s) 1668
AccB7I CCANNNNNTGG 2 cut(s) 2019, 2059
AccI GTMKAC 1 cut(s) 996
AccII CGCG 1 cut(s) 279
AccIII TCCGGA 1 cut(s) 1427
AciI CCGC 7 cut(s) 201, 277, 354, 401, 810, 925, 1663
AclWI GGATC 1 cut(s) 1027
AcoI YGGCCR 2 cut(s) 670, 1747
AcsI RAATTY 5 cut(s) 898, 1310, 1321, 2168, 2318
AcuI CTGAAG 1 cut(s) 2169
AfaI GTAC 7 cut(s) 11, 666, 734, 1040, 1167, 1340, 1484
AfeI AGCGCT 1 cut(s) 855
AfiI CCNNNNNNNGG 6 cut(s) 571, 936, 1456, 2019, 2059, 2293
AflIII ACRYGT 2 cut(s) 1335, 1638
AgsI TTSAA 6 cut(s) 169, 581, 590, 729, 1321, 1489
AhdI GACNNNNNGTC 1 cut(s) 1663
AjiI CACGTC 1 cut(s) 163
Alw21I GWGCWC 3 cut(s) 210, 524, 1228
Alw26I GTCTC 8 cut(s) 82, 87, 89, 404, 1406, 2079, 2176, 2331
AlwI GGATC 1 cut(s) 1027
AlwNI CAGNNNCTG 2 cut(s) 30, 1856
Ama87I CYCGRG 1 cut(s) 79
Aor13HI TCCGGA 1 cut(s) 1427
Aor51HI AGCGCT 1 cut(s) 855
AoxI GGCC 4 cut(s) 102, 670, 1129, 1747
ApeKI GCWGC 1 cut(s) 1760
ApoI RAATTY 5 cut(s) 898, 1310, 1321, 2168, 2318
AseI ATTAAT 2 cut(s) 1080, 1755
Asp700I GAANNNNTTC 2 cut(s) 218, 2319
AspLEI GCGC 2 cut(s) 856, 2027
AspS9I GGNCC 5 cut(s) 27, 361, 807, 927, 2215
AsuC2I CCSGG 2 cut(s) 460, 2220
AsuHPI GGTGA 7 cut(s) 350, 464, 496, 758, 1568, 1667, 1970
AsuII TTCGAA 1 cut(s) 1032
AvaI CYCGRG 1 cut(s) 79
AvaII GGWCC 5 cut(s) 27, 361, 807, 927, 2215
BalI TGGCCA 1 cut(s) 672
BanII GRGCYC 1 cut(s) 1911
BarI GAAGNNNNNNTAC 4 cut(s) 1149, 1181, 2285, 2317
BauI CACGAG 1 cut(s) 2334
BbsI GAAGAC 2 cut(s) 157, 1672
Bbv12I GWGCWC 3 cut(s) 210, 524, 1228
BbvCI CCTCAGC 1 cut(s) 621
BbvI GCAGC 1 cut(s) 1747
BccI CCATC 4 cut(s) 565, 695, 1684, 2053
BciVI GTATCC 2 cut(s) 2111, 2216
BclI TGATCA 1 cut(s) 1777
BcnI CCSGG 2 cut(s) 460, 2220
BcoDI GTCTC 8 cut(s) 82, 87, 89, 404, 1406, 2079, 2176, 2331
BfmI CTRYAG 1 cut(s) 2004
BfoI RGCGCY 2 cut(s) 857, 2028
BfuAI ACCTGC 1 cut(s) 1668
BfuI GTATCC 2 cut(s) 2111, 2216
BisI GCNGC 2 cut(s) 202, 1761
BlsI GCNGC 2 cut(s) 203, 1762
Bme1390I CCNGG 2 cut(s) 460, 2220
Bme18I GGWCC 5 cut(s) 27, 361, 807, 927, 2215
BmeRI GACNNNNNGTC 1 cut(s) 1663
BmeT110I CYCGRG 1 cut(s) 79
BmgBI CACGTC 1 cut(s) 163
BmgT120I GGNCC 5 cut(s) 27, 361, 807, 927, 2215
BmiI GGNNCC 3 cut(s) 142, 894, 2217
BmrFI CCNGG 2 cut(s) 460, 2220
BmrI ACTGGG 2 cut(s) 449, 2170
BmsI GCATC 7 cut(s) 388, 445, 809, 864, 1170, 1608, 1999
BmuI ACTGGG 2 cut(s) 449, 2170
BpiI GAAGAC 2 cut(s) 157, 1672
BpmI CTGGAG 1 cut(s) 2289
Bpu10I CCTNAGC 3 cut(s) 448, 621, 1671
Bpu14I TTCGAA 1 cut(s) 1032
BpuMI CCSGG 2 cut(s) 460, 2220
BsaI GGTCTC 4 cut(s) 87, 89, 404, 1406
BsaJI CCNNGG 6 cut(s) 364, 525, 1189, 1396, 1593, 2218
BsaWI WCCGGW 1 cut(s) 1427
Bsc4I CCNNNNNNNGG 6 cut(s) 571, 936, 1456, 2019, 2059, 2293
Bse118I RCCGGY 1 cut(s) 481
Bse1I ACTGG 5 cut(s) 444, 597, 841, 2119, 2165
Bse3DI GCAATG 3 cut(s) 483, 819, 970
BseAI TCCGGA 1 cut(s) 1427
BseDI CCNNGG 6 cut(s) 364, 525, 1189, 1396, 1593, 2218
BseGI GGATG 6 cut(s) 496, 576, 1312, 2045, 2064, 2170
BseLI CCNNNNNNNGG 6 cut(s) 571, 936, 1456, 2019, 2059, 2293
BseMI GCAATG 3 cut(s) 483, 819, 970
BseMII CTCAG 2 cut(s) 612, 1685
BseNI ACTGG 5 cut(s) 444, 597, 841, 2119, 2165
BseXI GCAGC 1 cut(s) 1747
BseYI CCCAGC 1 cut(s) 1730
BsgI GTGCAG 2 cut(s) 16, 1196
Bsh1236I CGCG 1 cut(s) 279
BshFI GGCC 4 cut(s) 104, 672, 1131, 1749
BsiHKAI GWGCWC 3 cut(s) 210, 524, 1228
BsiHKCI CYCGRG 1 cut(s) 79
BsiSI CCGG 5 cut(s) 460, 482, 1428, 1750, 2220
BslFI GGGAC 4 cut(s) 23, 806, 1068, 2201
BslI CCNNNNNNNGG 6 cut(s) 571, 936, 1456, 2019, 2059, 2293
BsmAI GTCTC 8 cut(s) 82, 87, 89, 404, 1406, 2079, 2176, 2331
BsmBI CGTCTC 2 cut(s) 82, 2079
BsmFI GGGAC 4 cut(s) 23, 806, 1068, 2201
BsmI GAATGC 1 cut(s) 1420
BsnI GGCC 4 cut(s) 104, 672, 1131, 1749
Bso31I GGTCTC 4 cut(s) 87, 89, 404, 1406
BsoBI CYCGRG 1 cut(s) 79
Bsp119I TTCGAA 1 cut(s) 1032
Bsp1286I GDGCHC 4 cut(s) 210, 524, 1228, 1911
Bsp13I TCCGGA 1 cut(s) 1427
Bsp1407I TGTACA 2 cut(s) 9, 732
Bsp19I CCATGG 2 cut(s) 364, 1189
BspACI CCGC 7 cut(s) 201, 277, 354, 401, 810, 925, 1663
BspANI GGCC 4 cut(s) 104, 672, 1131, 1749
BspCNI CTCAG 2 cut(s) 613, 1684
BspEI TCCGGA 1 cut(s) 1427
BspFNI CGCG 1 cut(s) 279
BspHI TCATGA 4 cut(s) 210, 1495, 1722, 1821
BspLI GGNNCC 3 cut(s) 142, 894, 2217
BspMI ACCTGC 1 cut(s) 1668
BspPI GGATC 1 cut(s) 1027
BspT104I TTCGAA 1 cut(s) 1032
BspTNI GGTCTC 4 cut(s) 87, 89, 404, 1406
BsrDI GCAATG 3 cut(s) 483, 819, 970
BsrFI RCCGGY 1 cut(s) 481
BsrGI TGTACA 2 cut(s) 9, 732
BsrI ACTGG 5 cut(s) 444, 597, 841, 2119, 2165
BssAI RCCGGY 1 cut(s) 481
BssECI CCNNGG 6 cut(s) 364, 525, 1189, 1396, 1593, 2218
BssSI CACGAG 1 cut(s) 2334
BssT1I CCWWGG 4 cut(s) 364, 1189, 1396, 1593
Bst2BI CACGAG 1 cut(s) 2334
Bst4CI ACNGT 5 cut(s) 37, 737, 1493, 2228, 2306
Bst6I CTCTTC 1 cut(s) 2286
BstAUI TGTACA 2 cut(s) 9, 732
BstBI TTCGAA 1 cut(s) 1032
BstC8I GCNNGC 4 cut(s) 206, 947, 1840, 2014
BstDEI CTNAG 7 cut(s) 448, 621, 885, 1058, 1671, 1946, 2207
BstDSI CCRYGG 3 cut(s) 364, 525, 1189
BstEII GGTNACC 3 cut(s) 904, 1445, 1655
BstENI CCTNNNNNAGG 1 cut(s) 934
BstF5I GGATG 6 cut(s) 496, 576, 1312, 2045, 2064, 2170
BstFNI CGCG 1 cut(s) 279
BstH2I RGCGCY 2 cut(s) 857, 2028
BstHHI GCGC 2 cut(s) 856, 2027
BstMAI GTCTC 8 cut(s) 82, 87, 89, 404, 1406, 2079, 2176, 2331
BstMWI GCNNNNNNNGC 5 cut(s) 285, 860, 1232, 2022, 2245
BstNSI RCATGY 4 cut(s) 16, 1219, 1339, 1642
BstPI GGTNACC 3 cut(s) 904, 1445, 1655
BstSCI CCNGG 2 cut(s) 458, 2218
BstSFI CTRYAG 1 cut(s) 2004
BstUI CGCG 1 cut(s) 279
BstV1I GCAGC 1 cut(s) 1747
BstV2I GAAGAC 2 cut(s) 157, 1672
BstX2I RGATCY 1 cut(s) 1019
BstYI RGATCY 1 cut(s) 1019
BsuI GTATCC 2 cut(s) 2111, 2216
BsuRI GGCC 4 cut(s) 104, 672, 1131, 1749
BtgI CCRYGG 3 cut(s) 364, 525, 1189
BtrI CACGTC 1 cut(s) 163
BtsCI GGATG 6 cut(s) 496, 576, 1312, 2045, 2064, 2170
BtsI GCAGTG 3 cut(s) 232, 795, 1911
BtsIMutI CAGTG 5 cut(s) 232, 795, 1325, 1911, 2112
BveI ACCTGC 1 cut(s) 1668
Cac8I GCNNGC 4 cut(s) 206, 947, 1840, 2014
CaiI CAGNNNCTG 2 cut(s) 30, 1856
CciI TCATGA 4 cut(s) 210, 1495, 1722, 1821
CfoI GCGC 2 cut(s) 856, 2027
Cfr10I RCCGGY 1 cut(s) 481
Cfr13I GGNCC 5 cut(s) 27, 361, 807, 927, 2215
CseI GACGC 2 cut(s) 482, 869
Csp6I GTAC 7 cut(s) 10, 665, 733, 1039, 1166, 1339, 1483
CviQI GTAC 7 cut(s) 10, 665, 733, 1039, 1166, 1339, 1483
DdeI CTNAG 7 cut(s) 448, 621, 885, 1058, 1671, 1946, 2207
DrdI GACNNNNNNGTC 1 cut(s) 703
DriI GACNNNNNGTC 1 cut(s) 1663
DseDI GACNNNNNNGTC 1 cut(s) 703
EaeI YGGCCR 2 cut(s) 670, 1747
Eam1104I CTCTTC 1 cut(s) 2286
Eam1105I GACNNNNNGTC 1 cut(s) 1663
EarI CTCTTC 1 cut(s) 2286
Eco130I CCWWGG 4 cut(s) 364, 1189, 1396, 1593
Eco147I AGGCCT 1 cut(s) 104
Eco24I GRGCYC 1 cut(s) 1911
Eco31I GGTCTC 4 cut(s) 87, 89, 404, 1406
Eco32I GATATC 2 cut(s) 819, 2098
Eco47I GGWCC 5 cut(s) 27, 361, 807, 927, 2215
Eco47III AGCGCT 1 cut(s) 855
Eco57I CTGAAG 1 cut(s) 2169
Eco88I CYCGRG 1 cut(s) 79
Eco91I GGTNACC 3 cut(s) 904, 1445, 1655
EcoNI CCTNNNNNAGG 1 cut(s) 934
EcoO109I RGGNCCY 2 cut(s) 27, 2215
EcoO65I GGTNACC 3 cut(s) 904, 1445, 1655
EcoRI GAATTC 2 cut(s) 898, 1321
EcoRV GATATC 2 cut(s) 819, 2098
EcoT14I CCWWGG 4 cut(s) 364, 1189, 1396, 1593
EcoT22I ATGCAT 1 cut(s) 1746
EcoT38I GRGCYC 1 cut(s) 1911
ErhI CCWWGG 4 cut(s) 364, 1189, 1396, 1593
Esp3I CGTCTC 2 cut(s) 82, 2079
FaqI GGGAC 4 cut(s) 23, 806, 1068, 2201
FauI CCCGC 1 cut(s) 347
FauNDI CATATG 3 cut(s) 1782, 1975, 2018
FbaI TGATCA 1 cut(s) 1777
FblI GTMKAC 1 cut(s) 996
Fnu4HI GCNGC 2 cut(s) 202, 1761
FokI GGATG 6 cut(s) 503, 583, 1319, 2052, 2071, 2177
FriOI GRGCYC 1 cut(s) 1911
Fsp4HI GCNGC 2 cut(s) 202, 1761
GlaI GCGC 2 cut(s) 855, 2026
GluI GCNGC 2 cut(s) 202, 1761
GsaI CCCAGC 1 cut(s) 1734
GsuI CTGGAG 1 cut(s) 2289
HaeII RGCGCY 2 cut(s) 857, 2028
HaeIII GGCC 4 cut(s) 104, 672, 1131, 1749
HapII CCGG 5 cut(s) 460, 482, 1428, 1750, 2220
HgaI GACGC 2 cut(s) 482, 869
HhaI GCGC 2 cut(s) 856, 2027
Hin6I GCGC 2 cut(s) 854, 2025
HinP1I GCGC 2 cut(s) 854, 2025
HincII GTYRAC 1 cut(s) 1939
HindII GTYRAC 1 cut(s) 1939
HindIII AAGCTT 1 cut(s) 1533
HpaII CCGG 5 cut(s) 460, 482, 1428, 1750, 2220
HphI GGTGA 7 cut(s) 350, 464, 496, 758, 1568, 1667, 1970
Hpy166II GTNNAC 6 cut(s) 997, 1576, 1617, 1939, 2254, 2302
Hpy188I TCNGA 5 cut(s) 397, 1140, 1272, 1543, 1883
Hpy8I GTNNAC 6 cut(s) 997, 1576, 1617, 1939, 2254, 2302
Hpy99I CGWCG 1 cut(s) 77
HpyCH4III ACNGT 5 cut(s) 37, 737, 1493, 2228, 2306
HpyCH4IV ACGT 2 cut(s) 162, 2088
HpyF10VI GCNNNNNNNGC 5 cut(s) 285, 860, 1232, 2022, 2245
HpyF3I CTNAG 7 cut(s) 448, 621, 885, 1058, 1671, 1946, 2207
HpySE526I ACGT 2 cut(s) 162, 2088
HspAI GCGC 2 cut(s) 854, 2025
Kpn2I TCCGGA 1 cut(s) 1427
Ksp22I TGATCA 1 cut(s) 1777
LmnI GCTCC 4 cut(s) 527, 1853, 1970, 2264
Lsp1109I GCAGC 1 cut(s) 1747
LweI GCATC 7 cut(s) 388, 445, 809, 864, 1170, 1608, 1999
MaeII ACGT 2 cut(s) 162, 2088
MaeIII GTNAC 9 cut(s) 601, 746, 781, 904, 1405, 1445, 1510, 1655, 1844
MboII GAAGA 5 cut(s) 144, 157, 1672, 1706, 2273
MfeI CAATTG 1 cut(s) 1086
MflI RGATCY 1 cut(s) 1019
MhlI GDGCHC 4 cut(s) 210, 524, 1228, 1911
MlsI TGGCCA 1 cut(s) 672
MluNI TGGCCA 1 cut(s) 672
MlyI GAGTC 3 cut(s) 386, 890, 1686
MmeI TCCRAC 2 cut(s) 534, 548
Mox20I TGGCCA 1 cut(s) 672
Mph1103I ATGCAT 1 cut(s) 1746
MroI TCCGGA 1 cut(s) 1427
MroXI GAANNNNTTC 2 cut(s) 218, 2319
MscI TGGCCA 1 cut(s) 672
MseI TTAA 7 cut(s) 693, 1080, 1389, 1520, 1755, 2034, 2079
MslI CAYNNNNRTG 3 cut(s) 672, 728, 1820
Msp20I TGGCCA 1 cut(s) 672
MspI CCGG 5 cut(s) 460, 482, 1428, 1750, 2220
MspR9I CCNGG 2 cut(s) 460, 2220
MunI CAATTG 1 cut(s) 1086
Mva1269I GAATGC 1 cut(s) 1420
MvnI CGCG 1 cut(s) 279
MwoI GCNNNNNNNGC 5 cut(s) 285, 860, 1232, 2022, 2245
NciI CCSGG 2 cut(s) 460, 2220
NcoI CCATGG 2 cut(s) 364, 1189
NdeI CATATG 3 cut(s) 1782, 1975, 2018
NlaIV GGNNCC 3 cut(s) 142, 894, 2217
NmeAIII GCCGAG 1 cut(s) 1211
NmuCI GTSAC 4 cut(s) 746, 1510, 1655, 1844
NsiI ATGCAT 1 cut(s) 1746
NspI RCATGY 4 cut(s) 16, 1219, 1339, 1642
NspV TTCGAA 1 cut(s) 1032
PagI TCATGA 4 cut(s) 210, 1495, 1722, 1821
PceI AGGCCT 1 cut(s) 104
PciI ACATGT 2 cut(s) 1335, 1638
PcsI WCGNNNNNNNCGW 3 cut(s) 69, 506, 515
PctI GAATGC 1 cut(s) 1420
PdmI GAANNNNTTC 2 cut(s) 218, 2319
PfeI GAWTC 7 cut(s) 122, 188, 229, 596, 1205, 1361, 2046
PflMI CCANNNNNTGG 2 cut(s) 2019, 2059
PkrI GCNGC 2 cut(s) 203, 1762
PleI GAGTC 3 cut(s) 386, 889, 1685
PpsI GAGTC 3 cut(s) 386, 889, 1685
PpuMI RGGWCCY 2 cut(s) 27, 2215
PscI ACATGT 2 cut(s) 1335, 1638
PshBI ATTAAT 2 cut(s) 1080, 1755
Psp5II RGGWCCY 2 cut(s) 27, 2215
PspEI GGTNACC 3 cut(s) 904, 1445, 1655
PspFI CCCAGC 1 cut(s) 1730
PspN4I GGNNCC 3 cut(s) 142, 894, 2217
PspPI GGNCC 5 cut(s) 27, 361, 807, 927, 2215
PspPPI RGGWCCY 2 cut(s) 27, 2215
PstNI CAGNNNCTG 2 cut(s) 30, 1856
PsuI RGATCY 1 cut(s) 1019
RsaI GTAC 7 cut(s) 11, 666, 734, 1040, 1167, 1340, 1484
RsaNI GTAC 7 cut(s) 10, 665, 733, 1039, 1166, 1339, 1483
RseI CAYNNNNRTG 3 cut(s) 672, 728, 1820
SaqAI TTAA 7 cut(s) 693, 1080, 1389, 1520, 1755, 2034, 2079
SatI GCNGC 2 cut(s) 202, 1761
Sau96I GGNCC 5 cut(s) 27, 361, 807, 927, 2215
SchI GAGTC 3 cut(s) 386, 890, 1686
ScrFI CCNGG 2 cut(s) 460, 2220
SduI GDGCHC 4 cut(s) 210, 524, 1228, 1911
SfaNI GCATC 7 cut(s) 388, 445, 809, 864, 1170, 1608, 1999
SfcI CTRYAG 1 cut(s) 2004
SfuI TTCGAA 1 cut(s) 1032
SinI GGWCC 5 cut(s) 27, 361, 807, 927, 2215
SmiMI CAYNNNNRTG 3 cut(s) 672, 728, 1820
SseBI AGGCCT 1 cut(s) 104
SsiI CCGC 7 cut(s) 201, 277, 354, 401, 810, 925, 1663
SspI AATATT 1 cut(s) 1828
StuI AGGCCT 1 cut(s) 104
StyD4I CCNGG 2 cut(s) 458, 2218
StyI CCWWGG 4 cut(s) 364, 1189, 1396, 1593
TaaI ACNGT 5 cut(s) 37, 737, 1493, 2228, 2306
TaiI ACGT 2 cut(s) 165, 2091
TaqI TCGA 9 cut(s) 408, 509, 1032, 1380, 1683, 1860, 1951, 2044, 2070
TaqII GACCGA 1 cut(s) 2192
TatI WGTACW 6 cut(s) 9, 664, 732, 1038, 1338, 1482
TauI GCSGC 1 cut(s) 204
TfiI GAWTC 7 cut(s) 122, 188, 229, 596, 1205, 1361, 2046
Tru1I TTAA 7 cut(s) 693, 1080, 1389, 1520, 1755, 2034, 2079
Tru9I TTAA 7 cut(s) 693, 1080, 1389, 1520, 1755, 2034, 2079
TscAI CASTG 5 cut(s) 239, 802, 1332, 1918, 2119
TseFI GTSAC 4 cut(s) 746, 1510, 1655, 1844
TseI GCWGC 1 cut(s) 1760
Tsp45I GTSAC 4 cut(s) 746, 1510, 1655, 1844
TspGWI ACGGA 3 cut(s) 514, 554, 1983
TspRI CASTG 5 cut(s) 239, 802, 1332, 1918, 2119
Van91I CCANNNNNTGG 2 cut(s) 2019, 2059
VpaK11BI GGWCC 5 cut(s) 27, 361, 807, 927, 2215
VspI ATTAAT 2 cut(s) 1080, 1755
XagI CCTNNNNNAGG 1 cut(s) 934
XapI RAATTY 5 cut(s) 898, 1310, 1321, 2168, 2318
XceI RCATGY 4 cut(s) 16, 1219, 1339, 1642
XmiI GTMKAC 1 cut(s) 996
XmnI GAANNNNTTC 2 cut(s) 218, 2319
Zsp2I ATGCAT 1 cut(s) 1746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.