AT1G69520

Hypothetical methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009895
Physical Location & Seq
Forward (+)
26128288 .. 26129602
1315 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G69520.2

Sequence Viewer

Length: 696 bp
ATGCTCGGTGTTCAAAGGATTTTCCAGGAGCACATCATCCGCAGAGGCCGGTGGTGTATTAGGAGGGTCTACTCGTGGTTTTTTAACTTGTTGATGCAGTCTTATTGGAACGAGATTGAGGATTGCAAGGTGAAGATATTTGATAAACTGAGTGAAAAAGCTGAAAAGGTTTTGGAGATTGGTATTGGCACAGGTCCTAATATGAGATACTACGCTGCTCGCAATAGTAACGTTACTCTATATGGGTTGGATCCAAATCCCAAAATGAAGAAATACGCACGCAAATCCGCTACCAAAGCAGGATTGAAACCTAAAAACTTTAGATTCAAGCAAGGAGTAGGAGAAGCTATACCATTAAAAGATAACTCTGTGGATGCAGTTGTTGCAACACTGGTTCTATGTTCTGTCTCTGATGTCACTCAAACACTCAAAGAAATCAAACGTGTGCTGAGACAAGGAGGGGTTTTCATCTTCTTAGAACATGTTGCAGCAAAAGATGGATCGTTCTTCAAGCGTTTGCAGAAATTGTTAGATCCGTTGCAGCAGAGACTTGCAGATGGATGCCATTTGGCAAGAAACACGAGAGAGTGCATCTTGGAAGCTGGTTTTAGTGGAGGAGTTGAAGTACAGACCTTTTCTATGTATAGCTTCCCTTGGATGACAAGACCTCATATCTATGGACTAGCTTACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

231

Amino Acids

26.51

Weight (kDa)

9.72

Isoelectric Point (pI)

40.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 62 - 185 2.3e-12 Methyltransferase domain
Methyltransf_31 PF13847 66 - 186 8.8e-18 Methyltransferase domain
Ubie_methyltran PF01209 66 - 175 1.1e-12 ubiE/COQ5 methyltransferase family
Methyltransf_25 PF13649 71 - 168 3.1e-19 Methyltransferase domain
Methyltransf_11 PF08241 72 - 172 5.9e-24 Methyltransferase domain
Methyltransf_12 PF08242 72 - 170 1.6e-14 Methyltransferase domain
Methyltransf_8 PF05148 130 - 174 3.7e-06 Hypothetical methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009895)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69520 AT1G69520 AT1G69523 AT1G69526 AT1G69526 AT1G69526
fragaria_vesca FvH4_4g31120 FvH4_4g31120
malus_domestica MD16G1070300.v1.1
prunus_persica Prupe.1G276900_v2.0.a1 Prupe.1G276900_v2.0.a1
pyrus_communis pycom16g06120
rosa_chinensis RchiOBHm_Chr4g0439511
rosa_laevigata RLG00000006262
rosa_multiflora Rmu_co8194290.1_g000001
rosa_roxburghii Rroxscaffold_5G00380430
rosa_samantha Rh4AG370300 Rh4BG382200 Rh4CG396800 Rh4DG376900
rosa_wichuraiana Rw4G031570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 69
AciI CCGC 2 cut(s) 40, 288
AclI AACGTT 1 cut(s) 231
AclWI GGATC 4 cut(s) 245, 258, 508, 527
AfaI GTAC 1 cut(s) 627
AflIII ACRYGT 2 cut(s) 442, 481
AgsI TTSAA 5 cut(s) 14, 307, 328, 511, 623
AjnI CCWGG 1 cut(s) 24
AluBI AGCT 5 cut(s) 161, 347, 602, 648, 686
AluI AGCT 5 cut(s) 161, 347, 602, 648, 686
Alw21I GWGCWC 1 cut(s) 33
Alw26I GTCTC 3 cut(s) 412, 445, 541
AlwI GGATC 4 cut(s) 245, 258, 508, 527
AoxI GGCC 1 cut(s) 46
ApeKI GCWGC 3 cut(s) 215, 488, 541
AspS9I GGNCC 1 cut(s) 194
AsuHPI GGTGA 1 cut(s) 142
AvaII GGWCC 1 cut(s) 194
BamHI GGATCC 1 cut(s) 250
BauI CACGAG 2 cut(s) 73, 580
Bbv12I GWGCWC 1 cut(s) 33
BbvI GCAGC 3 cut(s) 202, 500, 553
BccI CCATC 2 cut(s) 491, 551
BciT130I CCWGG 1 cut(s) 26
BcoDI GTCTC 3 cut(s) 412, 445, 541
BfaI CTAG 1 cut(s) 683
BisI GCNGC 3 cut(s) 216, 489, 542
BlsI GCNGC 3 cut(s) 217, 490, 543
Bme1390I CCNGG 1 cut(s) 26
Bme18I GGWCC 1 cut(s) 194
BmgT120I GGNCC 1 cut(s) 194
BmiI GGNNCC 1 cut(s) 252
BmrFI CCNGG 1 cut(s) 26
BmsI GCATC 4 cut(s) 84, 364, 551, 600
BsaBI GATNNNNATC 1 cut(s) 255
BsaJI CCNNGG 1 cut(s) 653
BsaXI ACNNNNNCTCC 2 cut(s) 609, 639
Bse118I RCCGGY 1 cut(s) 48
Bse1I ACTGG 1 cut(s) 396
Bse8I GATNNNNATC 1 cut(s) 255
BseBI CCWGG 1 cut(s) 26
BseDI CCNNGG 1 cut(s) 653
BseGI GGATG 4 cut(s) 36, 379, 566, 663
BseJI GATNNNNATC 1 cut(s) 255
BseMII CTCAG 2 cut(s) 140, 440
BseNI ACTGG 1 cut(s) 396
BseRI GAGGAG 1 cut(s) 630
BseXI GCAGC 3 cut(s) 202, 500, 553
BshFI GGCC 1 cut(s) 48
BsiHKAI GWGCWC 1 cut(s) 33
BsiSI CCGG 1 cut(s) 49
BsmAI GTCTC 3 cut(s) 412, 445, 541
BsnI GGCC 1 cut(s) 48
Bsp1286I GDGCHC 1 cut(s) 33
Bsp143I GATC 3 cut(s) 250, 500, 532
BspACI CCGC 2 cut(s) 40, 288
BspANI GGCC 1 cut(s) 48
BspCNI CTCAG 2 cut(s) 141, 441
BspLI GGNNCC 1 cut(s) 252
BspPI GGATC 4 cut(s) 245, 258, 508, 527
BsrFI RCCGGY 1 cut(s) 48
BsrI ACTGG 1 cut(s) 396
BssAI RCCGGY 1 cut(s) 48
BssECI CCNNGG 1 cut(s) 653
BssMI GATC 3 cut(s) 250, 500, 532
BssSI CACGAG 2 cut(s) 73, 580
BssT1I CCWWGG 1 cut(s) 653
Bst2BI CACGAG 2 cut(s) 73, 580
Bst2UI CCWGG 1 cut(s) 26
BstAPI GCANNNNNTGC 1 cut(s) 383
BstC8I GCNNGC 2 cut(s) 220, 280
BstDEI CTNAG 3 cut(s) 149, 449, 475
BstF5I GGATG 4 cut(s) 36, 379, 566, 663
BstKTI GATC 3 cut(s) 253, 503, 535
BstMAI GTCTC 3 cut(s) 412, 445, 541
BstMBI GATC 3 cut(s) 250, 500, 532
BstMWI GCNNNNNNNGC 2 cut(s) 296, 383
BstNI CCWGG 1 cut(s) 26
BstNSI RCATGY 1 cut(s) 485
BstSCI CCNGG 1 cut(s) 24
BstV1I GCAGC 3 cut(s) 202, 500, 553
BstX2I RGATCY 2 cut(s) 250, 532
BstYI RGATCY 2 cut(s) 250, 532
BsuRI GGCC 1 cut(s) 48
BtsCI GGATG 4 cut(s) 36, 379, 566, 663
BtsIMutI CAGTG 1 cut(s) 389
Cac8I GCNNGC 2 cut(s) 220, 280
Cfr10I RCCGGY 1 cut(s) 48
Cfr13I GGNCC 1 cut(s) 194
Csp6I GTAC 1 cut(s) 626
CviAII CATG 1 cut(s) 482
CviJI RGCY 6 cut(s) 48, 161, 347, 602, 648, 686
CviKI_1 RGCY 6 cut(s) 48, 161, 347, 602, 648, 686
CviQI GTAC 1 cut(s) 626
DdeI CTNAG 3 cut(s) 149, 449, 475
DpnI GATC 3 cut(s) 252, 502, 534
DpnII GATC 3 cut(s) 250, 500, 532
Eco130I CCWWGG 1 cut(s) 653
Eco47I GGWCC 1 cut(s) 194
EcoO109I RGGNCCY 1 cut(s) 194
EcoRII CCWGG 1 cut(s) 24
EcoT14I CCWWGG 1 cut(s) 653
ErhI CCWWGG 1 cut(s) 653
FaeI CATG 1 cut(s) 485
FatI CATG 1 cut(s) 481
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 3 cut(s) 216, 489, 542
FokI GGATG 4 cut(s) 23, 386, 573, 670
Fsp4HI GCNGC 3 cut(s) 216, 489, 542
FspBI CTAG 1 cut(s) 683
GluI GCNGC 3 cut(s) 216, 489, 542
HaeIII GGCC 1 cut(s) 48
HapII CCGG 1 cut(s) 49
Hin1II CATG 1 cut(s) 485
HinfI GANTC 1 cut(s) 324
HpaII CCGG 1 cut(s) 49
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 1 cut(s) 70
Hpy188I TCNGA 1 cut(s) 412
Hpy8I GTNNAC 1 cut(s) 70
HpyCH4IV ACGT 2 cut(s) 231, 442
HpyCH4V TGCA 9 cut(s) 97, 126, 377, 386, 488, 520, 541, 554, 591
HpyF10VI GCNNNNNNNGC 2 cut(s) 296, 383
HpyF3I CTNAG 3 cut(s) 149, 449, 475
HpySE526I ACGT 2 cut(s) 231, 442
Hsp92II CATG 1 cut(s) 485
Kzo9I GATC 3 cut(s) 250, 500, 532
LmnI GCTCC 1 cut(s) 28
LpnPI CCDG 7 cut(s) 11, 38, 62, 177, 285, 377, 588
Lsp1109I GCAGC 3 cut(s) 202, 500, 553
LweI GCATC 4 cut(s) 84, 364, 551, 600
MaeI CTAG 1 cut(s) 683
MaeII ACGT 2 cut(s) 231, 442
MaeIII GTNAC 3 cut(s) 227, 232, 415
MalI GATC 3 cut(s) 252, 502, 534
MboI GATC 3 cut(s) 250, 500, 532
MboII GAAGA 4 cut(s) 145, 280, 463, 499
MflI RGATCY 2 cut(s) 250, 532
MhlI GDGCHC 1 cut(s) 33
MluCI AATT 1 cut(s) 524
MmeI TCCRAC 1 cut(s) 228
MnlI CCTC 6 cut(s) 38, 57, 112, 452, 608, 678
MseI TTAA 2 cut(s) 84, 356
MslI CAYNNNNRTG 1 cut(s) 675
MspI CCGG 1 cut(s) 49
MspR9I CCNGG 1 cut(s) 26
MvaI CCWGG 1 cut(s) 26
MwoI GCNNNNNNNGC 2 cut(s) 296, 383
NdeII GATC 3 cut(s) 250, 500, 532
NlaIII CATG 1 cut(s) 485
NlaIV GGNNCC 1 cut(s) 252
NmuCI GTSAC 1 cut(s) 415
NspI RCATGY 1 cut(s) 485
PciI ACATGT 1 cut(s) 481
PfeI GAWTC 1 cut(s) 324
PfoI TCCNGGA 1 cut(s) 24
PkrI GCNGC 3 cut(s) 217, 490, 543
PpuMI RGGWCCY 1 cut(s) 194
PscI ACATGT 1 cut(s) 481
Psp1406I AACGTT 1 cut(s) 231
Psp5II RGGWCCY 1 cut(s) 194
Psp6I CCWGG 1 cut(s) 24
PspGI CCWGG 1 cut(s) 24
PspN4I GGNNCC 1 cut(s) 252
PspPI GGNCC 1 cut(s) 194
PspPPI RGGWCCY 1 cut(s) 194
PsuI RGATCY 2 cut(s) 250, 532
RsaI GTAC 1 cut(s) 627
RsaNI GTAC 1 cut(s) 626
RseI CAYNNNNRTG 1 cut(s) 675
SaqAI TTAA 2 cut(s) 84, 356
SatI GCNGC 3 cut(s) 216, 489, 542
Sau3AI GATC 3 cut(s) 250, 500, 532
Sau96I GGNCC 1 cut(s) 194
ScrFI CCNGG 1 cut(s) 26
SduI GDGCHC 1 cut(s) 33
SfaNI GCATC 4 cut(s) 84, 364, 551, 600
SinI GGWCC 1 cut(s) 194
SmiMI CAYNNNNRTG 1 cut(s) 675
Sse9I AATT 1 cut(s) 524
SsiI CCGC 2 cut(s) 40, 288
SspMI CTAG 1 cut(s) 683
StyD4I CCNGG 1 cut(s) 24
StyI CCWWGG 1 cut(s) 653
TaiI ACGT 2 cut(s) 234, 445
TasI AATT 1 cut(s) 524
TatI WGTACW 1 cut(s) 625
TfiI GAWTC 1 cut(s) 324
Tru1I TTAA 2 cut(s) 84, 356
Tru9I TTAA 2 cut(s) 84, 356
TscAI CASTG 1 cut(s) 396
TseFI GTSAC 1 cut(s) 415
TseI GCWGC 3 cut(s) 215, 488, 541
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 2 cut(s) 281, 457
TspGWI ACGGA 1 cut(s) 525
TspRI CASTG 1 cut(s) 396
VpaK11BI GGWCC 1 cut(s) 194
XceI RCATGY 1 cut(s) 485
XmiI GTMKAC 1 cut(s) 69
XspI CTAG 1 cut(s) 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.