AT1G69526

Hypothetical methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009895
Physical Location & Seq
Forward (+)
26131294 .. 26133313
2020 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G69526.2

Sequence Viewer

Length: 687 bp
ATGATGAAGATGATGACTCGAGTTCTTTCATCGCCTTCCTCACATGTCTCGTCGCTCAAATACTCACCTCGCAGAGTGATTCGAGCTTCACGAGATCATCTTCGTGCTGAAACTCTAAAGAGTCATCAACTTCCTTCAGGCTCCTCCTCCACCAGCTTATGTTCCTGTGGAAGAAAACATTTTCTCGAAGCAGCTAGCCCGACGATGCCCTTTCTTCCTATTTGTTCACCTAATGCTTCGCGTTCCAAGGATGTTTCAGAAACATTTCATCCTCAGAGGCCAGATTGGTATAAGGAGCTTTTTGCTTGGTTCTTGAGCACAGGAATGCGATCTTATGAAGCAGAGGTCAGTCTTTGTGATTTAAATGCATCATTCTATGATCCATTTCTTGAGAATGATAGATCTTTTTCATGTTTTATGTTTGTGTTATTTGGTAAGATCGCCGAGTACAAGAGGAAACTATTCGAAAAACTGGCTGGTAAAGCAGAAACAGTGCTGGAGATTGGTGTTGGTACTGGCCCTAATCTGAAGTACTTTGCTGGTAATGAAAACGTCTGTGTTTTTGGTATGGACCCGAACCATAAAATGGAAAAGTACGCATGTGAATCTGCCAGAGAAGCTGGGATGAAACCTGAAAACTTTAGATTCATGCATGGAGTAAGTAAATTGCTCTGTTTTGATTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

228

Amino Acids

25.84

Weight (kDa)

8.87

Isoelectric Point (pI)

53.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 114 - 238 1.1e-12 Methyltransferase domain
Methyltransf_31 PF13847 130 - 250 1.2e-14 Methyltransferase domain
Ubie_methyltran PF01209 131 - 261 4.8e-14 ubiE/COQ5 methyltransferase family
Methyltransf_25 PF13649 134 - 230 8.9e-17 Methyltransferase domain
Methyltransf_11 PF08241 135 - 234 3.2e-20 Methyltransferase domain
Methyltransf_12 PF08242 135 - 232 1e-10 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009895)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69520 AT1G69520 AT1G69523 AT1G69526 AT1G69526 AT1G69526
fragaria_vesca FvH4_4g31120 FvH4_4g31120
malus_domestica MD16G1070300.v1.1
prunus_persica Prupe.1G276900_v2.0.a1 Prupe.1G276900_v2.0.a1
pyrus_communis pycom16g06120
rosa_chinensis RchiOBHm_Chr4g0439511
rosa_laevigata RLG00000006262
rosa_multiflora Rmu_co8194290.1_g000001
rosa_roxburghii Rroxscaffold_5G00380430
rosa_samantha Rh4AG370300 Rh4BG382200 Rh4CG396800 Rh4DG376900
rosa_wichuraiana Rw4G031570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 586
AccII CGCG 1 cut(s) 241
AclWI GGATC 1 cut(s) 374
AcuI CTGAAG 2 cut(s) 120, 548
AfaI GTAC 4 cut(s) 449, 514, 533, 596
AfiI CCNNNNNNNGG 1 cut(s) 586
AflIII ACRYGT 1 cut(s) 43
AluBI AGCT 5 cut(s) 86, 156, 194, 298, 620
AluI AGCT 5 cut(s) 86, 156, 194, 298, 620
Alw21I GWGCWC 1 cut(s) 320
Alw26I GTCTC 1 cut(s) 52
AlwI GGATC 1 cut(s) 374
Ama87I CYCGRG 1 cut(s) 18
AoxI GGCC 2 cut(s) 278, 517
ApeKI GCWGC 1 cut(s) 191
Asp700I GAANNNNTTC 2 cut(s) 264, 461
AspS9I GGNCC 2 cut(s) 518, 571
AsuHPI GGTGA 2 cut(s) 57, 219
AsuII TTCGAA 1 cut(s) 465
AsuNHI GCTAGC 1 cut(s) 194
AvaI CYCGRG 1 cut(s) 18
AvaII GGWCC 1 cut(s) 571
BauI CACGAG 1 cut(s) 90
Bbv12I GWGCWC 1 cut(s) 320
BbvI GCAGC 1 cut(s) 203
BcoDI GTCTC 1 cut(s) 52
BfaI CTAG 1 cut(s) 195
BglII AGATCT 1 cut(s) 401
BisI GCNGC 1 cut(s) 192
BlsI GCNGC 1 cut(s) 193
BmcAI AGTACT 1 cut(s) 533
Bme18I GGWCC 1 cut(s) 571
BmeT110I CYCGRG 1 cut(s) 18
BmgT120I GGNCC 2 cut(s) 518, 571
BmiI GGNNCC 2 cut(s) 142, 573
BmsI GCATC 2 cut(s) 195, 377
BmtI GCTAGC 1 cut(s) 198
BpmI CTGGAG 1 cut(s) 518
Bpu14I TTCGAA 1 cut(s) 465
BpuEI CTTGAG 2 cut(s) 334, 410
BsaJI CCNNGG 1 cut(s) 246
Bsc4I CCNNNNNNNGG 1 cut(s) 586
Bse1I ACTGG 2 cut(s) 477, 520
BseDI CCNNGG 1 cut(s) 246
BseGI GGATG 3 cut(s) 256, 268, 630
BseLI CCNNNNNNNGG 1 cut(s) 586
BseMII CTCAG 1 cut(s) 287
BseNI ACTGG 2 cut(s) 477, 520
BseRI GAGGAG 2 cut(s) 133, 136
BseXI GCAGC 1 cut(s) 203
BseYI CCCAGC 1 cut(s) 620
Bsh1236I CGCG 1 cut(s) 241
BshFI GGCC 2 cut(s) 280, 519
BsiHKAI GWGCWC 1 cut(s) 320
BsiHKCI CYCGRG 1 cut(s) 18
BslI CCNNNNNNNGG 1 cut(s) 586
BsmAI GTCTC 1 cut(s) 52
BsmI GAATGC 1 cut(s) 330
BsnI GGCC 2 cut(s) 280, 519
BsoBI CYCGRG 1 cut(s) 18
Bsp119I TTCGAA 1 cut(s) 465
Bsp1286I GDGCHC 1 cut(s) 320
Bsp143I GATC 5 cut(s) 94, 329, 379, 401, 438
BspANI GGCC 2 cut(s) 280, 519
BspCNI CTCAG 1 cut(s) 286
BspFNI CGCG 1 cut(s) 241
BspLI GGNNCC 2 cut(s) 142, 573
BspOI GCTAGC 1 cut(s) 198
BspPI GGATC 1 cut(s) 374
BspT104I TTCGAA 1 cut(s) 465
BsrI ACTGG 2 cut(s) 477, 520
BssECI CCNNGG 1 cut(s) 246
BssMI GATC 5 cut(s) 94, 329, 379, 401, 438
BssSI CACGAG 1 cut(s) 90
BssT1I CCWWGG 1 cut(s) 246
Bst2BI CACGAG 1 cut(s) 90
Bst4CI ACNGT 1 cut(s) 493
BstBI TTCGAA 1 cut(s) 465
BstC8I GCNNGC 1 cut(s) 196
BstDEI CTNAG 1 cut(s) 273
BstF5I GGATG 3 cut(s) 256, 268, 630
BstFNI CGCG 1 cut(s) 241
BstKTI GATC 5 cut(s) 97, 332, 382, 404, 441
BstMAI GTCTC 1 cut(s) 52
BstMBI GATC 5 cut(s) 94, 329, 379, 401, 438
BstMWI GCNNNNNNNGC 2 cut(s) 482, 617
BstNSI RCATGY 2 cut(s) 47, 603
BstUI CGCG 1 cut(s) 241
BstV1I GCAGC 1 cut(s) 203
BstX2I RGATCY 1 cut(s) 401
BstYI RGATCY 1 cut(s) 401
BsuRI GGCC 2 cut(s) 280, 519
BtgZI GCGATG 1 cut(s) 15
BtsCI GGATG 3 cut(s) 256, 268, 630
BtsIMutI CAGTG 1 cut(s) 498
Cac8I GCNNGC 1 cut(s) 196
Cfr13I GGNCC 2 cut(s) 518, 571
Csp6I GTAC 4 cut(s) 448, 513, 532, 595
CviAII CATG 5 cut(s) 44, 411, 600, 649, 653
CviQI GTAC 4 cut(s) 448, 513, 532, 595
DdeI CTNAG 1 cut(s) 273
DpnI GATC 5 cut(s) 96, 331, 381, 403, 440
DpnII GATC 5 cut(s) 94, 329, 379, 401, 438
DraI TTTAAA 1 cut(s) 363
Eco130I CCWWGG 1 cut(s) 246
Eco47I GGWCC 1 cut(s) 571
Eco57I CTGAAG 2 cut(s) 120, 548
Eco88I CYCGRG 1 cut(s) 18
EcoT14I CCWWGG 1 cut(s) 246
EcoT22I ATGCAT 2 cut(s) 370, 654
ErhI CCWWGG 1 cut(s) 246
FaeI CATG 5 cut(s) 47, 414, 603, 652, 656
FatI CATG 5 cut(s) 43, 410, 599, 648, 652
Fnu4HI GCNGC 1 cut(s) 192
FokI GGATG 3 cut(s) 255, 263, 637
Fsp4HI GCNGC 1 cut(s) 192
FspBI CTAG 1 cut(s) 195
GluI GCNGC 1 cut(s) 192
GsaI CCCAGC 1 cut(s) 624
GsuI CTGGAG 1 cut(s) 518
HaeIII GGCC 2 cut(s) 280, 519
Hin1II CATG 5 cut(s) 47, 414, 603, 652, 656
HinfI GANTC 5 cut(s) 16, 79, 121, 605, 645
HphI GGTGA 2 cut(s) 57, 219
Hpy166II GTNNAC 1 cut(s) 227
Hpy188I TCNGA 3 cut(s) 259, 276, 528
Hpy188III TCNNGA 4 cut(s) 90, 185, 313, 389
Hpy8I GTNNAC 1 cut(s) 227
Hpy99I CGWCG 2 cut(s) 55, 205
HpyAV CCTTC 2 cut(s) 45, 144
HpyCH4III ACNGT 1 cut(s) 493
HpyCH4IV ACGT 1 cut(s) 552
HpyCH4V TGCA 2 cut(s) 368, 652
HpyF10VI GCNNNNNNNGC 2 cut(s) 482, 617
HpyF3I CTNAG 1 cut(s) 273
HpySE526I ACGT 1 cut(s) 552
Hsp92II CATG 5 cut(s) 47, 414, 603, 652, 656
Kzo9I GATC 5 cut(s) 94, 329, 379, 401, 438
LmnI GCTCC 2 cut(s) 146, 295
Lsp1109I GCAGC 1 cut(s) 203
LweI GCATC 2 cut(s) 195, 377
MaeI CTAG 1 cut(s) 195
MaeII ACGT 1 cut(s) 552
MalI GATC 5 cut(s) 96, 331, 381, 403, 440
MboI GATC 5 cut(s) 94, 329, 379, 401, 438
MboII GAAGA 4 cut(s) 19, 92, 183, 206
MflI RGATCY 1 cut(s) 401
MhlI GDGCHC 1 cut(s) 320
MluCI AATT 1 cut(s) 665
MlyI GAGTC 2 cut(s) 10, 130
MnlI CCTC 8 cut(s) 49, 78, 154, 157, 270, 282, 337, 447
Mph1103I ATGCAT 2 cut(s) 370, 654
MroXI GAANNNNTTC 2 cut(s) 264, 461
MseI TTAA 1 cut(s) 362
MslI CAYNNNNRTG 2 cut(s) 102, 323
Mva1269I GAATGC 1 cut(s) 330
MvnI CGCG 1 cut(s) 241
MwoI GCNNNNNNNGC 2 cut(s) 482, 617
NdeII GATC 5 cut(s) 94, 329, 379, 401, 438
NheI GCTAGC 1 cut(s) 194
NlaIII CATG 5 cut(s) 47, 414, 603, 652, 656
NlaIV GGNNCC 2 cut(s) 142, 573
NmeAIII GCCGAG 1 cut(s) 469
NsiI ATGCAT 2 cut(s) 370, 654
NspI RCATGY 2 cut(s) 47, 603
NspV TTCGAA 1 cut(s) 465
PaeR7I CTCGAG 1 cut(s) 18
PciI ACATGT 1 cut(s) 43
PcsI WCGNNNNNNNCGW 1 cut(s) 88
PctI GAATGC 1 cut(s) 330
PdmI GAANNNNTTC 2 cut(s) 264, 461
PfeI GAWTC 3 cut(s) 79, 605, 645
PflMI CCANNNNNTGG 1 cut(s) 586
PkrI GCNGC 1 cut(s) 193
PleI GAGTC 2 cut(s) 10, 129
PpsI GAGTC 2 cut(s) 10, 129
PscI ACATGT 1 cut(s) 43
PspFI CCCAGC 1 cut(s) 620
PspN4I GGNNCC 2 cut(s) 142, 573
PspPI GGNCC 2 cut(s) 518, 571
PspXI VCTCGAGB 1 cut(s) 18
PsuI RGATCY 1 cut(s) 401
RsaI GTAC 4 cut(s) 449, 514, 533, 596
RsaNI GTAC 4 cut(s) 448, 513, 532, 595
RseI CAYNNNNRTG 2 cut(s) 102, 323
SaqAI TTAA 1 cut(s) 362
SatI GCNGC 1 cut(s) 192
Sau3AI GATC 5 cut(s) 94, 329, 379, 401, 438
Sau96I GGNCC 2 cut(s) 518, 571
ScaI AGTACT 1 cut(s) 533
SchI GAGTC 2 cut(s) 10, 130
SduI GDGCHC 1 cut(s) 320
SfaNI GCATC 2 cut(s) 195, 377
Sfr274I CTCGAG 1 cut(s) 18
SfuI TTCGAA 1 cut(s) 465
SinI GGWCC 1 cut(s) 571
SlaI CTCGAG 1 cut(s) 18
SmiI ATTTAAAT 1 cut(s) 363
SmiMI CAYNNNNRTG 2 cut(s) 102, 323
SmlI CTYRAG 3 cut(s) 18, 313, 389
SmoI CTYRAG 3 cut(s) 18, 313, 389
Sse9I AATT 1 cut(s) 665
SspMI CTAG 1 cut(s) 195
StyI CCWWGG 1 cut(s) 246
SwaI ATTTAAAT 1 cut(s) 363
TaaI ACNGT 1 cut(s) 493
TaiI ACGT 1 cut(s) 555
TaqI TCGA 4 cut(s) 19, 82, 186, 465
TasI AATT 1 cut(s) 665
TatI WGTACW 2 cut(s) 447, 531
TfiI GAWTC 3 cut(s) 79, 605, 645
Tru1I TTAA 1 cut(s) 362
Tru9I TTAA 1 cut(s) 362
TscAI CASTG 1 cut(s) 498
TseI GCWGC 1 cut(s) 191
TspDTI ATGAA 8 cut(s) 18, 20, 257, 351, 399, 561, 637, 641
TspRI CASTG 1 cut(s) 498
Van91I CCANNNNNTGG 1 cut(s) 586
VpaK11BI GGWCC 1 cut(s) 571
XceI RCATGY 2 cut(s) 47, 603
XhoI CTCGAG 1 cut(s) 18
XmnI GAANNNNTTC 2 cut(s) 264, 461
XspI CTAG 1 cut(s) 195
ZrmI AGTACT 1 cut(s) 533
Zsp2I ATGCAT 2 cut(s) 370, 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.