MD16G1070300.v1.1

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009895
Physical Location & Seq
Forward (+)
4933469 .. 4935773
2305 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1070300.v1.1.491

Sequence Viewer

Length: 990 bp
ATGATCCATTGCGCCATTCCCTCCATGTCCAACATCGCGCCACGTGGCCTAATCACGTCGGGTTCCAATCCTTCTCCGACCCGAGTCAACTCGGATTCTTCAGCCGACGGGTCACTGGCGCCGGCCTCGCTCTGCGTGAGCAGCGGATTATGTTCGTGCGGAAGACGGCGCTTCATCGAAGCAGCAGTGGCGACGCCATCGTTATTCCCAATTCGCCCTTCCAAAGCCTCCGCGGCTTCCAAATTGACTTCTGAAGATTATACGGCCATGTTAGAAGAAGTTCACCCTCCAAGACCGGGCTGGTACGAGGAGCTTTATGCATCCGTTTTGAATACCGCCATGAAATCTTATGAAGCCGAGATTGCAGGTTACAAGACAGAGCTCTTTGCTGAATTGAAGGGAAAAGCTGAGCAAGTGTTGGAAATTGGTATTGGGACTGGTCCTAATCTGAGATACTATGCTGCTGATAGTGGTGTTCGGGTTTTTGGCGTGGATCCGATTATGAAGATGGAAAAATATGCTCGGGCTGCTGCTGTGGCTGCCGGTCTGCCCCTTTCGAATTTCGAGTTCATACATGCTGTATGTGAAAACTTCAAACTTCCCTTATGGCAGTGCAGAAAAACACAGAAACTGGTTGTTGGGGAGGCTATTCCATTAGAGGATGCTTCTGTTGATGCAGTTGTTGGAACTCTTGTGTTGTGTTCTGTTAAAGATGTCGACAACACTCTGAAAGAGATAAAGAGAGTTCTAAGACCGGGCGGAGTTTACCTGTTCGTGGAGCATGTGGCTGCAAAAGATGGTACAATCCTCAGATTCATACAGAGCGTTCTTAATCCTCTGCAACAAATCCTAGCCGACGGGTGTCATCTCACCCGGGACACAGGAAGGAGTATATCCAACTCCGGGTTCGCGGCCGTTAATCTACAGACGACCACCTTGTCCAGTGCCGCATTCGTAAACCCTCAAGTGTATGGAATAGCTCGTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

330

Amino Acids

35.28

Weight (kDa)

8.06

Isoelectric Point (pI)

40.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 125 - 264 9.4e-12 Methyltransferase domain
Methyltransf_31 PF13847 135 - 263 1.6e-06 Methyltransferase domain
Methyltransf_25 PF13649 139 - 201 3.9e-06 Methyltransferase domain
Methyltransf_11 PF08241 140 - 258 2.3e-16 Methyltransferase domain
Methyltransf_12 PF08242 140 - 255 9.3e-09 Methyltransferase domain
Methyltransf_8 PF05148 216 - 263 7e-06 Hypothetical methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009895)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69520 AT1G69520 AT1G69523 AT1G69526 AT1G69526 AT1G69526
fragaria_vesca FvH4_4g31120 FvH4_4g31120
malus_domestica MD16G1070300.v1.1
prunus_persica Prupe.1G276900_v2.0.a1 Prupe.1G276900_v2.0.a1
pyrus_communis pycom16g06120
rosa_chinensis RchiOBHm_Chr4g0439511
rosa_laevigata RLG00000006262
rosa_multiflora Rmu_co8194290.1_g000001
rosa_roxburghii Rroxscaffold_5G00380430
rosa_samantha Rh4AG370300 Rh4BG382200 Rh4CG396800 Rh4DG376900
rosa_wichuraiana Rw4G031570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 937
Acc36I ACCTGC 1 cut(s) 356
AccB1I GGYRCC 1 cut(s) 118
AccI GTMKAC 1 cut(s) 717
AccII CGCG 3 cut(s) 38, 233, 911
AciI CCGC 8 cut(s) 144, 159, 231, 233, 336, 759, 911, 948
AclWI GGATC 2 cut(s) 488, 501
AcoI YGGCCR 2 cut(s) 264, 912
AcsI RAATTY 1 cut(s) 559
AcuI CTGAAG 2 cut(s) 84, 273
AcvI CACGTG 1 cut(s) 44
AcyI GRCGYC 2 cut(s) 119, 194
AfaI GTAC 2 cut(s) 305, 802
AfiI CCNNNNNNNGG 3 cut(s) 296, 775, 903
AgsI TTSAA 3 cut(s) 331, 397, 595
AjiI CACGTC 1 cut(s) 57
AjuI GAANNNNNNNTTGG 6 cut(s) 202, 234, 414, 446, 890, 922
AluBI AGCT 4 cut(s) 313, 382, 407, 980
AluI AGCT 4 cut(s) 313, 382, 407, 980
Alw21I GWGCWC 1 cut(s) 384
AlwI GGATC 2 cut(s) 488, 501
AlwNI CAGNNNCTG 1 cut(s) 631
Ama87I CYCGRG 3 cut(s) 81, 522, 873
AoxI GGCC 4 cut(s) 46, 123, 264, 912
ApeKI GCWGC 7 cut(s) 141, 182, 461, 527, 530, 539, 788
ApoI RAATTY 1 cut(s) 559
Asp700I GAANNNNTTC 1 cut(s) 279
AspLEI GCGC 4 cut(s) 14, 40, 121, 171
AspS9I GGNCC 1 cut(s) 440
AsuC2I CCSGG 5 cut(s) 297, 756, 874, 875, 904
AsuHPI GGTGA 2 cut(s) 275, 862
AsuII TTCGAA 1 cut(s) 557
AvaI CYCGRG 3 cut(s) 81, 522, 873
AvaII GGWCC 1 cut(s) 440
BamHI GGATCC 1 cut(s) 493
BanI GGYRCC 1 cut(s) 118
BanII GRGCYC 1 cut(s) 384
BbrPI CACGTG 1 cut(s) 44
BbsI GAAGAC 1 cut(s) 169
Bbv12I GWGCWC 1 cut(s) 384
BbvI GCAGC 7 cut(s) 153, 194, 448, 514, 517, 526, 775
BccI CCATC 3 cut(s) 205, 502, 791
BceAI ACGGC 3 cut(s) 182, 279, 899
BcnI CCSGG 5 cut(s) 297, 756, 874, 875, 904
BfaI CTAG 1 cut(s) 851
BfmI CTRYAG 1 cut(s) 923
BfoI RGCGCY 2 cut(s) 122, 172
BfuAI ACCTGC 1 cut(s) 356
BglI GCCNNNNNGGC 1 cut(s) 233
BlpI GCTNAGC 1 cut(s) 408
Bme1390I CCNGG 5 cut(s) 297, 756, 874, 875, 904
Bme18I GGWCC 1 cut(s) 440
BmeT110I CYCGRG 3 cut(s) 81, 522, 873
BmgBI CACGTC 1 cut(s) 57
BmgT120I GGNCC 1 cut(s) 440
BmiI GGNNCC 3 cut(s) 64, 120, 495
BmrFI CCNGG 5 cut(s) 297, 756, 874, 875, 904
BmsI GCATC 3 cut(s) 329, 652, 664
BoxI GACNNNNGTC 2 cut(s) 83, 861
BpiI GAAGAC 1 cut(s) 169
Bpu1102I GCTNAGC 1 cut(s) 408
Bpu14I TTCGAA 1 cut(s) 557
BpuEI CTTGAG 1 cut(s) 948
BpuMI CCSGG 5 cut(s) 297, 756, 874, 875, 904
BsaAI YACGTR 1 cut(s) 44
BsaHI GRCGYC 2 cut(s) 119, 194
BsaJI CCNNGG 2 cut(s) 231, 873
Bsc4I CCNNNNNNNGG 3 cut(s) 296, 775, 903
Bse118I RCCGGY 2 cut(s) 121, 542
Bse1I ACTGG 4 cut(s) 120, 442, 636, 942
Bse3DI GCAATG 1 cut(s) 7
BseDI CCNNGG 2 cut(s) 231, 873
BseGI GGATG 2 cut(s) 320, 667
BseLI CCNNNNNNNGG 3 cut(s) 296, 775, 903
BseMI GCAATG 1 cut(s) 7
BseMII CTCAG 3 cut(s) 399, 440, 823
BseNI ACTGG 4 cut(s) 120, 442, 636, 942
BseRI GAGGAG 1 cut(s) 323
BseX3I CGGCCG 1 cut(s) 912
BseXI GCAGC 7 cut(s) 153, 194, 448, 514, 517, 526, 775
BsgI GTGCAG 1 cut(s) 634
Bsh1236I CGCG 3 cut(s) 38, 233, 911
Bsh1285I CGRYCG 1 cut(s) 915
BshFI GGCC 4 cut(s) 48, 125, 266, 914
BshNI GGYRCC 1 cut(s) 118
BsiEI CGRYCG 1 cut(s) 915
BsiHKAI GWGCWC 1 cut(s) 384
BsiHKCI CYCGRG 3 cut(s) 81, 522, 873
BsiSI CCGG 6 cut(s) 122, 296, 543, 755, 874, 903
BslFI GGGAC 2 cut(s) 448, 890
BslI CCNNNNNNNGG 3 cut(s) 296, 775, 903
BsmFI GGGAC 2 cut(s) 448, 890
BsmI GAATGC 1 cut(s) 950
BsnI GGCC 4 cut(s) 48, 125, 266, 914
BsoBI CYCGRG 3 cut(s) 81, 522, 873
Bsp119I TTCGAA 1 cut(s) 557
Bsp1286I GDGCHC 1 cut(s) 384
Bsp143I GATC 2 cut(s) 3, 493
Bsp1720I GCTNAGC 1 cut(s) 408
BspACI CCGC 8 cut(s) 144, 159, 231, 233, 336, 759, 911, 948
BspANI GGCC 4 cut(s) 48, 125, 266, 914
BspCNI CTCAG 3 cut(s) 400, 441, 822
BspFNI CGCG 3 cut(s) 38, 233, 911
BspLI GGNNCC 3 cut(s) 64, 120, 495
BspMI ACCTGC 1 cut(s) 356
BspPI GGATC 2 cut(s) 488, 501
BspT104I TTCGAA 1 cut(s) 557
BspT107I GGYRCC 1 cut(s) 118
BsrDI GCAATG 1 cut(s) 7
BsrFI RCCGGY 2 cut(s) 121, 542
BsrI ACTGG 4 cut(s) 120, 442, 636, 942
BssAI RCCGGY 2 cut(s) 121, 542
BssECI CCNNGG 2 cut(s) 231, 873
BssMI GATC 2 cut(s) 3, 493
BssNI GRCGYC 2 cut(s) 119, 194
BstACI GRCGYC 2 cut(s) 119, 194
BstBAI YACGTR 1 cut(s) 44
BstBI TTCGAA 1 cut(s) 557
BstC8I GCNNGC 1 cut(s) 123
BstDEI CTNAG 4 cut(s) 408, 449, 749, 809
BstDSI CCRYGG 1 cut(s) 231
BstF5I GGATG 2 cut(s) 320, 667
BstFNI CGCG 3 cut(s) 38, 233, 911
BstH2I RGCGCY 2 cut(s) 122, 172
BstHHI GCGC 4 cut(s) 14, 40, 121, 171
BstKTI GATC 2 cut(s) 6, 496
BstMBI GATC 2 cut(s) 3, 493
BstMCI CGRYCG 1 cut(s) 915
BstMWI GCNNNNNNNGC 8 cut(s) 127, 141, 188, 233, 362, 527, 536, 539
BstNSI RCATGY 2 cut(s) 578, 785
BstPAI GACNNNNGTC 2 cut(s) 83, 861
BstSCI CCNGG 5 cut(s) 295, 754, 872, 873, 902
BstSFI CTRYAG 1 cut(s) 923
BstUI CGCG 3 cut(s) 38, 233, 911
BstV1I GCAGC 7 cut(s) 153, 194, 448, 514, 517, 526, 775
BstV2I GAAGAC 1 cut(s) 169
BstX2I RGATCY 1 cut(s) 493
BstYI RGATCY 1 cut(s) 493
BstZI CGGCCG 1 cut(s) 912
BsuRI GGCC 4 cut(s) 48, 125, 266, 914
BtgI CCRYGG 1 cut(s) 231
BtgZI GCGATG 1 cut(s) 19
BtrI CACGTC 1 cut(s) 57
BtsCI GGATG 2 cut(s) 320, 667
BtsI GCAGTG 2 cut(s) 192, 617
BtsIMutI CAGTG 4 cut(s) 113, 192, 617, 949
BveI ACCTGC 1 cut(s) 356
Cac8I GCNNGC 1 cut(s) 123
CaiI CAGNNNCTG 1 cut(s) 631
CfoI GCGC 4 cut(s) 14, 40, 121, 171
Cfr10I RCCGGY 2 cut(s) 121, 542
Cfr13I GGNCC 1 cut(s) 440
Cfr42I CCGCGG 1 cut(s) 234
Cfr9I CCCGGG 1 cut(s) 873
CseI GACGC 1 cut(s) 202
Csp6I GTAC 2 cut(s) 304, 801
CviAII CATG 5 cut(s) 25, 268, 340, 575, 782
CviQI GTAC 2 cut(s) 304, 801
DdeI CTNAG 4 cut(s) 408, 449, 749, 809
DinI GGCGCC 1 cut(s) 120
DpnI GATC 2 cut(s) 5, 495
DpnII GATC 2 cut(s) 3, 493
DrdI GACNNNNNNGTC 1 cut(s) 937
DseDI GACNNNNNNGTC 1 cut(s) 937
EaeI YGGCCR 2 cut(s) 264, 912
EagI CGGCCG 1 cut(s) 912
EciI GGCGGA 1 cut(s) 774
Ecl136II GAGCTC 1 cut(s) 382
EclXI CGGCCG 1 cut(s) 912
Eco24I GRGCYC 1 cut(s) 384
Eco47I GGWCC 1 cut(s) 440
Eco52I CGGCCG 1 cut(s) 912
Eco53kI GAGCTC 1 cut(s) 382
Eco57I CTGAAG 2 cut(s) 84, 273
Eco72I CACGTG 1 cut(s) 44
Eco88I CYCGRG 3 cut(s) 81, 522, 873
EcoICRI GAGCTC 1 cut(s) 382
EcoT22I ATGCAT 1 cut(s) 322
EcoT38I GRGCYC 1 cut(s) 384
EgeI GGCGCC 1 cut(s) 120
EheI GGCGCC 1 cut(s) 120
FaeI CATG 5 cut(s) 28, 271, 343, 578, 785
FaqI GGGAC 2 cut(s) 448, 890
FatI CATG 5 cut(s) 24, 267, 339, 574, 781
FblI GTMKAC 1 cut(s) 717
FokI GGATG 2 cut(s) 307, 674
FriOI GRGCYC 1 cut(s) 384
FspBI CTAG 1 cut(s) 851
GlaI GCGC 4 cut(s) 13, 39, 120, 170
HaeII RGCGCY 2 cut(s) 122, 172
HaeIII GGCC 4 cut(s) 48, 125, 266, 914
HapII CCGG 6 cut(s) 122, 296, 543, 755, 874, 903
HgaI GACGC 1 cut(s) 202
HhaI GCGC 4 cut(s) 14, 40, 121, 171
Hin1I GRCGYC 2 cut(s) 119, 194
Hin1II CATG 5 cut(s) 28, 271, 343, 578, 785
Hin6I GCGC 4 cut(s) 12, 38, 119, 169
HinP1I GCGC 4 cut(s) 12, 38, 119, 169
HincII GTYRAC 2 cut(s) 88, 718
HindII GTYRAC 2 cut(s) 88, 718
HinfI GANTC 3 cut(s) 84, 95, 813
HpaII CCGG 6 cut(s) 122, 296, 543, 755, 874, 903
HphI GGTGA 2 cut(s) 275, 862
Hpy166II GTNNAC 5 cut(s) 88, 283, 718, 766, 958
Hpy188I TCNGA 7 cut(s) 78, 94, 253, 450, 498, 729, 812
Hpy8I GTNNAC 5 cut(s) 88, 283, 718, 766, 958
Hpy99I CGWCG 4 cut(s) 61, 110, 196, 860
HpyAV CCTTC 4 cut(s) 81, 228, 391, 879
HpyCH4IV ACGT 2 cut(s) 43, 56
HpyCH4V TGCA 6 cut(s) 320, 365, 615, 677, 791, 841
HpyF10VI GCNNNNNNNGC 8 cut(s) 127, 141, 188, 233, 362, 527, 536, 539
HpyF3I CTNAG 4 cut(s) 408, 449, 749, 809
HpySE526I ACGT 2 cut(s) 43, 56
Hsp92I GRCGYC 2 cut(s) 119, 194
Hsp92II CATG 5 cut(s) 28, 271, 343, 578, 785
HspAI GCGC 4 cut(s) 12, 38, 119, 169
KasI GGCGCC 1 cut(s) 118
KroI GCCGGC 1 cut(s) 121
KroNI GCCGGC 1 cut(s) 123
KspI CCGCGG 1 cut(s) 234
Kzo9I GATC 2 cut(s) 3, 493
LmnI GCTCC 2 cut(s) 310, 778
Lsp1109I GCAGC 7 cut(s) 153, 194, 448, 514, 517, 526, 775
LweI GCATC 3 cut(s) 329, 652, 664
MaeI CTAG 1 cut(s) 851
MaeII ACGT 2 cut(s) 43, 56
MaeIII GTNAC 2 cut(s) 111, 368
MalI GATC 2 cut(s) 5, 495
MboI GATC 2 cut(s) 3, 493
MboII GAAGA 5 cut(s) 90, 174, 266, 287, 517
MflI RGATCY 1 cut(s) 493
MhlI GDGCHC 1 cut(s) 384
MluCI AATT 5 cut(s) 210, 242, 392, 423, 559
Mly113I GGCGCC 1 cut(s) 119
MlyI GAGTC 1 cut(s) 93
MmeI TCCRAC 5 cut(s) 54, 101, 399, 664, 921
Mph1103I ATGCAT 1 cut(s) 322
MroNI GCCGGC 1 cut(s) 121
MroXI GAANNNNTTC 1 cut(s) 279
MseI TTAA 3 cut(s) 708, 831, 918
MspA1I CMGCKG 2 cut(s) 144, 233
MspI CCGG 6 cut(s) 122, 296, 543, 755, 874, 903
MspR9I CCNGG 5 cut(s) 297, 756, 874, 875, 904
Mva1269I GAATGC 1 cut(s) 950
MvnI CGCG 3 cut(s) 38, 233, 911
MwoI GCNNNNNNNGC 8 cut(s) 127, 141, 188, 233, 362, 527, 536, 539
NaeI GCCGGC 1 cut(s) 123
NarI GGCGCC 1 cut(s) 119
NciI CCSGG 5 cut(s) 297, 756, 874, 875, 904
NdeII GATC 2 cut(s) 3, 493
NgoMIV GCCGGC 1 cut(s) 121
NlaIII CATG 5 cut(s) 28, 271, 343, 578, 785
NlaIV GGNNCC 3 cut(s) 64, 120, 495
NmeAIII GCCGAG 1 cut(s) 382
NmuCI GTSAC 1 cut(s) 111
NsiI ATGCAT 1 cut(s) 322
NspI RCATGY 2 cut(s) 578, 785
NspV TTCGAA 1 cut(s) 557
PctI GAATGC 1 cut(s) 950
PdiI GCCGGC 1 cut(s) 123
PdmI GAANNNNTTC 1 cut(s) 279
PfeI GAWTC 2 cut(s) 95, 813
PleI GAGTC 1 cut(s) 92
PluTI GGCGCC 1 cut(s) 122
PmaCI CACGTG 1 cut(s) 44
PmlI CACGTG 1 cut(s) 44
PpsI GAGTC 1 cut(s) 92
Ppu21I YACGTR 1 cut(s) 44
PshAI GACNNNNGTC 2 cut(s) 83, 861
Psp124BI GAGCTC 1 cut(s) 384
PspCI CACGTG 1 cut(s) 44
PspN4I GGNNCC 3 cut(s) 64, 120, 495
PspPI GGNCC 1 cut(s) 440
PstNI CAGNNNCTG 1 cut(s) 631
PsuI RGATCY 1 cut(s) 493
RsaI GTAC 2 cut(s) 305, 802
RsaNI GTAC 2 cut(s) 304, 801
SacI GAGCTC 1 cut(s) 384
SacII CCGCGG 1 cut(s) 234
SalI GTCGAC 1 cut(s) 716
SaqAI TTAA 3 cut(s) 708, 831, 918
Sau3AI GATC 2 cut(s) 3, 493
Sau96I GGNCC 1 cut(s) 440
SchI GAGTC 1 cut(s) 93
ScrFI CCNGG 5 cut(s) 297, 756, 874, 875, 904
SduI GDGCHC 1 cut(s) 384
SetI ASST 9 cut(s) 46, 59, 315, 370, 384, 409, 771, 938, 982
SfaNI GCATC 3 cut(s) 329, 652, 664
SfcI CTRYAG 1 cut(s) 923
SfoI GGCGCC 1 cut(s) 120
Sfr303I CCGCGG 1 cut(s) 234
SfuI TTCGAA 1 cut(s) 557
SgrBI CCGCGG 1 cut(s) 234
SinI GGWCC 1 cut(s) 440
SmaI CCCGGG 1 cut(s) 875
SmlI CTYRAG 1 cut(s) 963
SmoI CTYRAG 1 cut(s) 963
Sse9I AATT 5 cut(s) 210, 242, 392, 423, 559
SsiI CCGC 8 cut(s) 144, 159, 231, 233, 336, 759, 911, 948
SspDI GGCGCC 1 cut(s) 118
SspMI CTAG 1 cut(s) 851
SstI GAGCTC 1 cut(s) 384
StyD4I CCNGG 5 cut(s) 295, 754, 872, 873, 902
TaiI ACGT 2 cut(s) 46, 59
TaqI TCGA 4 cut(s) 177, 557, 564, 717
TasI AATT 5 cut(s) 210, 242, 392, 423, 559
TauI GCSGC 3 cut(s) 236, 914, 950
TfiI GAWTC 2 cut(s) 95, 813
Tru1I TTAA 3 cut(s) 708, 831, 918
Tru9I TTAA 3 cut(s) 708, 831, 918
TscAI CASTG 4 cut(s) 120, 192, 617, 949
TseFI GTSAC 1 cut(s) 111
TseI GCWGC 7 cut(s) 141, 182, 461, 527, 530, 539, 788
Tsp45I GTSAC 1 cut(s) 111
TspDTI ATGAA 6 cut(s) 163, 356, 366, 518, 559, 805
TspGWI ACGGA 1 cut(s) 313
TspMI CCCGGG 1 cut(s) 873
TspRI CASTG 4 cut(s) 120, 192, 617, 949
VpaK11BI GGWCC 1 cut(s) 440
XapI RAATTY 1 cut(s) 559
XceI RCATGY 2 cut(s) 578, 785
XcmI CCANNNNNNNNNTGG 1 cut(s) 297
XmaI CCCGGG 1 cut(s) 873
XmiI GTMKAC 1 cut(s) 717
XmnI GAANNNNTTC 1 cut(s) 279
XspI CTAG 1 cut(s) 851
Zsp2I ATGCAT 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.