RchiOBHm_Chr4g0439511

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0009895
Physical Location & Seq
Forward (+)
61924347 .. 61926646
2300 bp
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UTR
Exon/CDS
Intron
PRQ40756

Sequence Viewer

Length: 924 bp
ATGATCCTTTACGGTCCTCTCTTCTCATCCGGCGTTACGCCACGTGGCAAAACCACATCTTCTTCTTCTTCTTCTCCGACCCGAGTCAACTCGGACGCTTCACCGGTCGGATCACTCGGGCCGGACTCGTTCTGTCCGAGCGGCTTATGTTCTTGCGGAAGAAGACGCTTCATCGAAGCAGCAGTCACTTCATCTCTATTCCCAATTTGCCCCTCCACCTCCTCCGCTTCCAAGTCGCAGTTCGACGATTATACGGCCACGTTGAGGAAAGTTCACCCTCCTCGACCAGACTGGTATGAAGAGTTGTATGCGTCAGCGTTGAACACCGGCATGCAATCTTACGAAGAAGAGATTGCGGGTTACAAGTCGGAGCTCTTTGCTAAGTTGAAGGGAAAAGCTGAGCAAGTGTTGGAAATTGGTATAGGGACTGGTCCTAATCTAAGATACTATGGTGCTGATGATGGTGTCCGAGTTTTCGGCGTGGATCCTAATAAAAAGATGGAAAGATATGCTAAGGCTGCGGCAGTGGCTGCTGGTCTGCCCCTTTCGAACTTTGAGTTTATACAAGCAGTTGGGGAGGCTATACCATTAGATGATGCTTCTGTTGATGCAGTTGTTGGAACCCTAGTATTGTGTTCTGTAAAAGATGTCAACAAGACTCTAAAAGAGATTAAGAGAGTGCTGAGACCAGGTGGACTCTACTTGTTTGTGGAGCATGTGGCTGCTAAAGATGGGACAGCCCTCAGATTTATACAGAGTGTTCTTGATCCTTTGCAACAAACCCTTGCCGATGGGTGTCATTTCACCCGGGAAACAGGAACCAATATATCCAGATCCGGCTTCTCAGGCGTCGAGCTTAGCATGACCTCCTTGTCCAGTACCTCAATTATAAACCCTCAAATCTATGGAATAGCTTGCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

307

Amino Acids

32.82

Weight (kDa)

6.83

Isoelectric Point (pI)

44.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 116 - 244 7.1e-13 Methyltransferase domain
Methyltransf_31 PF13847 132 - 246 4.5e-11 Methyltransferase domain
Methyltransf_25 PF13649 136 - 232 3.1e-17 Methyltransferase domain
Methyltransf_11 PF08241 137 - 236 8.4e-22 Methyltransferase domain
Methyltransf_12 PF08242 137 - 233 9.7e-12 Methyltransferase domain
Ubie_methyltran PF01209 152 - 239 5.5e-10 ubiE/COQ5 methyltransferase family
Methyltransf_8 PF05148 194 - 238 5.6e-06 Hypothetical methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009895)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69520 AT1G69520 AT1G69523 AT1G69526 AT1G69526 AT1G69526
fragaria_vesca FvH4_4g31120 FvH4_4g31120
malus_domestica MD16G1070300.v1.1
prunus_persica Prupe.1G276900_v2.0.a1 Prupe.1G276900_v2.0.a1
pyrus_communis pycom16g06120
rosa_chinensis RchiOBHm_Chr4g0439511
rosa_laevigata RLG00000006262
rosa_multiflora Rmu_co8194290.1_g000001
rosa_roxburghii Rroxscaffold_5G00380430
rosa_samantha Rh4AG370300 Rh4BG382200 Rh4CG396800 Rh4DG376900
rosa_wichuraiana Rw4G031570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 890
AasI GACNNNNNNGTC 1 cut(s) 871
AccBSI CCGCTC 1 cut(s) 141
AciI CCGC 5 cut(s) 141, 156, 225, 356, 521
AclWI GGATC 5 cut(s) 118, 479, 492, 761, 828
AcoI YGGCCR 1 cut(s) 255
AcvI CACGTG 1 cut(s) 44
AcyI GRCGYC 1 cut(s) 849
AfaI GTAC 1 cut(s) 880
AfiI CCNNNNNNNGG 1 cut(s) 264
AgeI ACCGGT 1 cut(s) 103
AgsI TTSAA 2 cut(s) 322, 388
AjnI CCWGG 1 cut(s) 688
AjuI GAANNNNNNNTTGG 2 cut(s) 224, 256
AluBI AGCT 4 cut(s) 373, 398, 856, 914
AluI AGCT 4 cut(s) 373, 398, 856, 914
Alw21I GWGCWC 1 cut(s) 375
Alw26I GTCTC 1 cut(s) 679
AlwI GGATC 5 cut(s) 118, 479, 492, 761, 828
AlwNI CAGNNNCTG 1 cut(s) 530
Ama87I CYCGRG 3 cut(s) 81, 116, 807
AoxI GGCC 2 cut(s) 119, 255
ApeKI GCWGC 4 cut(s) 179, 518, 530, 722
ArsI GACNNNNNNTTYG 2 cut(s) 168, 200
AsiGI ACCGGT 1 cut(s) 103
AspS9I GGNCC 3 cut(s) 14, 119, 431
AsuC2I CCSGG 2 cut(s) 808, 809
AsuHPI GGTGA 3 cut(s) 93, 266, 796
AsuII TTCGAA 1 cut(s) 548
AvaI CYCGRG 3 cut(s) 81, 116, 807
AvaII GGWCC 2 cut(s) 14, 431
BaeI ACNNNNGTAYC 2 cut(s) 436, 469
BamHI GGATCC 1 cut(s) 484
BanII GRGCYC 1 cut(s) 375
BbrPI CACGTG 1 cut(s) 44
BbsI GAAGAC 1 cut(s) 169
Bbv12I GWGCWC 1 cut(s) 375
BbvI GCAGC 4 cut(s) 191, 505, 517, 709
BccI CCATC 4 cut(s) 455, 493, 725, 785
BceAI ACGGC 1 cut(s) 270
BciT130I CCWGG 1 cut(s) 690
BcnI CCSGG 2 cut(s) 808, 809
BcoDI GTCTC 1 cut(s) 679
BfaI CTAG 1 cut(s) 626
BisI GCNGC 6 cut(s) 142, 180, 519, 522, 531, 723
BlpI GCTNAGC 2 cut(s) 399, 857
BlsI GCNGC 6 cut(s) 143, 181, 520, 523, 532, 724
Bme1390I CCNGG 3 cut(s) 690, 808, 809
Bme18I GGWCC 2 cut(s) 14, 431
BmeT110I CYCGRG 3 cut(s) 81, 116, 807
BmgT120I GGNCC 3 cut(s) 14, 119, 431
BmiI GGNNCC 3 cut(s) 486, 622, 820
BmrFI CCNGG 3 cut(s) 690, 808, 809
BmsI GCATC 2 cut(s) 586, 598
BoxI GACNNNNGTC 1 cut(s) 83
BpiI GAAGAC 1 cut(s) 169
Bpu10I CCTNAGC 1 cut(s) 513
Bpu1102I GCTNAGC 2 cut(s) 399, 857
Bpu14I TTCGAA 1 cut(s) 548
BpuMI CCSGG 2 cut(s) 808, 809
BsaAI YACGTR 1 cut(s) 44
BsaHI GRCGYC 1 cut(s) 849
BsaI GGTCTC 1 cut(s) 679
BsaJI CCNNGG 1 cut(s) 807
BsaWI WCCGGW 1 cut(s) 103
Bsc4I CCNNNNNNNGG 1 cut(s) 264
Bse118I RCCGGY 2 cut(s) 103, 326
Bse1I ACTGG 3 cut(s) 296, 433, 876
BseBI CCWGG 1 cut(s) 690
BseDI CCNNGG 1 cut(s) 807
BseGI GGATG 1 cut(s) 26
BseLI CCNNNNNNNGG 1 cut(s) 264
BseMII CTCAG 4 cut(s) 390, 674, 757, 858
BseNI ACTGG 3 cut(s) 296, 433, 876
BseRI GAGGAG 2 cut(s) 211, 270
BseXI GCAGC 4 cut(s) 191, 505, 517, 709
Bsh1285I CGRYCG 1 cut(s) 108
BshFI GGCC 2 cut(s) 121, 257
BshTI ACCGGT 1 cut(s) 103
BsiEI CGRYCG 1 cut(s) 108
BsiHKAI GWGCWC 1 cut(s) 375
BsiHKCI CYCGRG 3 cut(s) 81, 116, 807
BsiSI CCGG 6 cut(s) 30, 104, 122, 327, 808, 837
BslFI GGGAC 2 cut(s) 439, 748
BslI CCNNNNNNNGG 1 cut(s) 264
BsmAI GTCTC 1 cut(s) 679
BsmFI GGGAC 2 cut(s) 439, 748
BsnI GGCC 2 cut(s) 121, 257
Bso31I GGTCTC 1 cut(s) 679
BsoBI CYCGRG 3 cut(s) 81, 116, 807
Bsp119I TTCGAA 1 cut(s) 548
Bsp1286I GDGCHC 1 cut(s) 375
Bsp143I GATC 5 cut(s) 3, 110, 484, 766, 833
Bsp1720I GCTNAGC 2 cut(s) 399, 857
BspACI CCGC 5 cut(s) 141, 156, 225, 356, 521
BspANI GGCC 2 cut(s) 121, 257
BspCNI CTCAG 4 cut(s) 391, 675, 756, 857
BspLI GGNNCC 3 cut(s) 486, 622, 820
BspPI GGATC 5 cut(s) 118, 479, 492, 761, 828
BspT104I TTCGAA 1 cut(s) 548
BspTNI GGTCTC 1 cut(s) 679
BsrBI CCGCTC 1 cut(s) 141
BsrFI RCCGGY 2 cut(s) 103, 326
BsrI ACTGG 3 cut(s) 296, 433, 876
BssAI RCCGGY 2 cut(s) 103, 326
BssECI CCNNGG 1 cut(s) 807
BssMI GATC 5 cut(s) 3, 110, 484, 766, 833
BssNI GRCGYC 1 cut(s) 849
Bst2UI CCWGG 1 cut(s) 690
Bst4CI ACNGT 1 cut(s) 14
Bst6I CTCTTC 3 cut(s) 26, 294, 342
BstACI GRCGYC 1 cut(s) 849
BstAPI GCANNNNNTGC 1 cut(s) 530
BstBAI YACGTR 1 cut(s) 44
BstBI TTCGAA 1 cut(s) 548
BstC8I GCNNGC 2 cut(s) 332, 916
BstDEI CTNAG 8 cut(s) 381, 399, 440, 513, 683, 743, 844, 857
BstF5I GGATG 1 cut(s) 26
BstKTI GATC 5 cut(s) 6, 113, 487, 769, 836
BstMAI GTCTC 1 cut(s) 679
BstMBI GATC 5 cut(s) 3, 110, 484, 766, 833
BstMCI CGRYCG 1 cut(s) 108
BstMWI GCNNNNNNNGC 4 cut(s) 518, 527, 530, 846
BstNI CCWGG 1 cut(s) 690
BstNSI RCATGY 2 cut(s) 334, 719
BstPAI GACNNNNGTC 1 cut(s) 83
BstSCI CCNGG 3 cut(s) 688, 806, 807
BstV1I GCAGC 4 cut(s) 191, 505, 517, 709
BstV2I GAAGAC 1 cut(s) 169
BstX2I RGATCY 2 cut(s) 484, 833
BstYI RGATCY 2 cut(s) 484, 833
BsuRI GGCC 2 cut(s) 121, 257
BtsCI GGATG 1 cut(s) 26
BtsI GCAGTG 1 cut(s) 531
BtsIMutI CAGTG 1 cut(s) 531
Cac8I GCNNGC 2 cut(s) 332, 916
CaiI CAGNNNCTG 1 cut(s) 530
Cfr10I RCCGGY 2 cut(s) 103, 326
Cfr13I GGNCC 3 cut(s) 14, 119, 431
Cfr9I CCCGGG 1 cut(s) 807
CseI GACGC 4 cut(s) 104, 174, 300, 838
CsiI ACCWGGT 1 cut(s) 688
Csp6I GTAC 1 cut(s) 879
CspAI ACCGGT 1 cut(s) 103
CviAII CATG 3 cut(s) 331, 716, 862
CviQI GTAC 1 cut(s) 879
DdeI CTNAG 8 cut(s) 381, 399, 440, 513, 683, 743, 844, 857
DpnI GATC 5 cut(s) 5, 112, 486, 768, 835
DpnII GATC 5 cut(s) 3, 110, 484, 766, 833
DrdI GACNNNNNNGTC 1 cut(s) 871
DseDI GACNNNNNNGTC 1 cut(s) 871
EaeI YGGCCR 1 cut(s) 255
Eam1104I CTCTTC 3 cut(s) 26, 294, 342
EarI CTCTTC 3 cut(s) 26, 294, 342
Ecl136II GAGCTC 1 cut(s) 373
Eco24I GRGCYC 1 cut(s) 375
Eco31I GGTCTC 1 cut(s) 679
Eco47I GGWCC 2 cut(s) 14, 431
Eco53kI GAGCTC 1 cut(s) 373
Eco72I CACGTG 1 cut(s) 44
Eco88I CYCGRG 3 cut(s) 81, 116, 807
EcoICRI GAGCTC 1 cut(s) 373
EcoRII CCWGG 1 cut(s) 688
EcoT38I GRGCYC 1 cut(s) 375
FaeI CATG 3 cut(s) 334, 719, 865
FaqI GGGAC 2 cut(s) 439, 748
FatI CATG 3 cut(s) 330, 715, 861
FauI CCCGC 1 cut(s) 349
Fnu4HI GCNGC 6 cut(s) 142, 180, 519, 522, 531, 723
FokI GGATG 1 cut(s) 13
FriOI GRGCYC 1 cut(s) 375
Fsp4HI GCNGC 6 cut(s) 142, 180, 519, 522, 531, 723
FspBI CTAG 1 cut(s) 626
GluI GCNGC 6 cut(s) 142, 180, 519, 522, 531, 723
HaeIII GGCC 2 cut(s) 121, 257
HapII CCGG 6 cut(s) 30, 104, 122, 327, 808, 837
HgaI GACGC 4 cut(s) 104, 174, 300, 838
Hin1I GRCGYC 1 cut(s) 849
Hin1II CATG 3 cut(s) 334, 719, 865
HincII GTYRAC 2 cut(s) 88, 652
HindII GTYRAC 2 cut(s) 88, 652
HinfI GANTC 4 cut(s) 84, 125, 658, 696
HpaII CCGG 6 cut(s) 30, 104, 122, 327, 808, 837
HphI GGTGA 3 cut(s) 93, 266, 796
Hpy166II GTNNAC 4 cut(s) 88, 274, 652, 695
Hpy188I TCNGA 7 cut(s) 78, 94, 110, 138, 370, 470, 746
Hpy188III TCNNGA 2 cut(s) 764, 831
Hpy8I GTNNAC 4 cut(s) 88, 274, 652, 695
Hpy99I CGWCG 2 cut(s) 248, 854
HpyAV CCTTC 1 cut(s) 382
HpyCH4III ACNGT 1 cut(s) 14
HpyCH4IV ACGT 2 cut(s) 43, 260
HpyCH4V TGCA 4 cut(s) 334, 611, 775, 918
HpyF10VI GCNNNNNNNGC 4 cut(s) 518, 527, 530, 846
HpyF3I CTNAG 8 cut(s) 381, 399, 440, 513, 683, 743, 844, 857
HpySE526I ACGT 2 cut(s) 43, 260
Hsp92I GRCGYC 1 cut(s) 849
Hsp92II CATG 3 cut(s) 334, 719, 865
Kzo9I GATC 5 cut(s) 3, 110, 484, 766, 833
LmnI GCTCC 2 cut(s) 370, 712
Lsp1109I GCAGC 4 cut(s) 191, 505, 517, 709
LweI GCATC 2 cut(s) 586, 598
MabI ACCWGGT 1 cut(s) 688
MaeI CTAG 1 cut(s) 626
MaeII ACGT 2 cut(s) 43, 260
MaeIII GTNAC 3 cut(s) 34, 184, 359
MalI GATC 5 cut(s) 5, 112, 486, 768, 835
MbiI CCGCTC 1 cut(s) 141
MboI GATC 5 cut(s) 3, 110, 484, 766, 833
MflI RGATCY 2 cut(s) 484, 833
MhlI GDGCHC 1 cut(s) 375
MluCI AATT 3 cut(s) 204, 414, 885
MlyI GAGTC 4 cut(s) 93, 119, 652, 690
MmeI TCCRAC 5 cut(s) 88, 101, 348, 390, 598
MseI TTAA 1 cut(s) 672
MslI CAYNNNNRTG 1 cut(s) 329
MspI CCGG 6 cut(s) 30, 104, 122, 327, 808, 837
MspR9I CCNGG 3 cut(s) 690, 808, 809
MvaI CCWGG 1 cut(s) 690
MwoI GCNNNNNNNGC 4 cut(s) 518, 527, 530, 846
NciI CCSGG 2 cut(s) 808, 809
NdeII GATC 5 cut(s) 3, 110, 484, 766, 833
NlaIII CATG 3 cut(s) 334, 719, 865
NlaIV GGNNCC 3 cut(s) 486, 622, 820
NmuCI GTSAC 1 cut(s) 184
NspI RCATGY 2 cut(s) 334, 719
NspV TTCGAA 1 cut(s) 548
PaeI GCATGC 1 cut(s) 334
PcsI WCGNNNNNNNCGW 1 cut(s) 134
PinAI ACCGGT 1 cut(s) 103
PkrI GCNGC 6 cut(s) 143, 181, 520, 523, 532, 724
PleI GAGTC 4 cut(s) 92, 119, 652, 690
PmaCI CACGTG 1 cut(s) 44
PmlI CACGTG 1 cut(s) 44
PpsI GAGTC 4 cut(s) 92, 119, 652, 690
Ppu21I YACGTR 1 cut(s) 44
PshAI GACNNNNGTC 1 cut(s) 83
PsiI TTATAA 1 cut(s) 890
Psp124BI GAGCTC 1 cut(s) 375
Psp6I CCWGG 1 cut(s) 688
PspCI CACGTG 1 cut(s) 44
PspGI CCWGG 1 cut(s) 688
PspN4I GGNNCC 3 cut(s) 486, 622, 820
PspPI GGNCC 3 cut(s) 14, 119, 431
PstNI CAGNNNCTG 1 cut(s) 530
PsuI RGATCY 2 cut(s) 484, 833
RsaI GTAC 1 cut(s) 880
RsaNI GTAC 1 cut(s) 879
RseI CAYNNNNRTG 1 cut(s) 329
SacI GAGCTC 1 cut(s) 375
SaqAI TTAA 1 cut(s) 672
SatI GCNGC 6 cut(s) 142, 180, 519, 522, 531, 723
Sau3AI GATC 5 cut(s) 3, 110, 484, 766, 833
Sau96I GGNCC 3 cut(s) 14, 119, 431
SchI GAGTC 4 cut(s) 93, 119, 652, 690
ScrFI CCNGG 3 cut(s) 690, 808, 809
SduI GDGCHC 1 cut(s) 375
SexAI ACCWGGT 1 cut(s) 688
SfaNI GCATC 2 cut(s) 586, 598
SfuI TTCGAA 1 cut(s) 548
SinI GGWCC 2 cut(s) 14, 431
SmaI CCCGGG 1 cut(s) 809
SmiMI CAYNNNNRTG 1 cut(s) 329
SphI GCATGC 1 cut(s) 334
Sse9I AATT 3 cut(s) 204, 414, 885
SsiI CCGC 5 cut(s) 141, 156, 225, 356, 521
SspMI CTAG 1 cut(s) 626
SstI GAGCTC 1 cut(s) 375
StyD4I CCNGG 3 cut(s) 688, 806, 807
TaaI ACNGT 1 cut(s) 14
TaiI ACGT 2 cut(s) 46, 263
TaqI TCGA 5 cut(s) 174, 243, 283, 548, 852
TasI AATT 3 cut(s) 204, 414, 885
TauI GCSGC 2 cut(s) 144, 524
Tru1I TTAA 1 cut(s) 672
Tru9I TTAA 1 cut(s) 672
TscAI CASTG 1 cut(s) 531
TseFI GTSAC 1 cut(s) 184
TseI GCWGC 4 cut(s) 179, 518, 530, 722
Tsp45I GTSAC 1 cut(s) 184
TspDTI ATGAA 3 cut(s) 160, 180, 312
TspMI CCCGGG 1 cut(s) 807
TspRI CASTG 1 cut(s) 531
VpaK11BI GGWCC 2 cut(s) 14, 431
XceI RCATGY 2 cut(s) 334, 719
XmaI CCCGGG 1 cut(s) 807
XspI CTAG 1 cut(s) 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.