Rroxscaffold_5G00380430

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0009895
Physical Location & Seq
Forward (+)
60594127 .. 60596305
2179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00380430.1

Sequence Viewer

Length: 918 bp
ATGATCCTTTACAGTCCTCTCTTCTCATCCGGCGTTACGCCACGTGGCAAAACCACATCTTCTTCTTCTCCGACCCGAGTCAACTGGGAAGGTTCAGCGGCCGGGTCACTCGGGCCGGACTCGTTCTGTCCGAGCGGCTTATGTTCTTGCGGAAGAAGACGCTTCATCGAAGCAGCAGTCACTTCATCTCTATTCCCAATTTGCCCCTCCACCTCCGTCGCTTCCAAGTCGCAGTACGACGATTATACGGCCACGTTGAGGAAAGTTCACCCTCCTCGACCGGACTGGTATGAAGAGTTGTATGCGTCGGTGTTGAACACCGGCATGCAATCTTACGAAGACGAGATTGCGGGTTACAAGTCGGAGCTCTTTGCTAAGTTGAAGGGAAAAGCTGAGCAAGTGTTGGAAATTGGTATAGGGACTGGTCCTAATCTAAGATACTATGGTGCTGATGATGGTGTCCGAGTTTTCGGCGTGGATCCTAATAAAAAGATGGAAAGATATGCTAAGGCTGCGGCCGTGGCTGCTGGTCTGCCCCTTCCGAACTTTGAGTTTATACAAGCAGTTGGGGAGGCTATACCATTAGATGATGCTTCTGTTGATGCAGTTGTTGGAACCCTTGTATTGTGTTCCGTAAAAGATGTCAACAAGACTCTAAAAGAGATTAAGAGAGTGCTGAGACCAGGTGGACTCTACTTGTTTGTGGAGCATGTGGCTGCAAAAGATGGGACAACCCTCAGATTTATACAGAGTGTTCTTGATCCTTTGCAACAAACGCTTGCCGATGGGTGTCATCTCACCCGGGAAACAGGAACCAATATATCCAGATCCGGCTTCTCAGGCGTCGAGCTTAGTATGACCTCCTTGTCCAGTACCTCAATTATAAACCCTCAAATCTATGGAATAGCTTGCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

305

Amino Acids

32.77

Weight (kDa)

6.83

Isoelectric Point (pI)

42.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 116 - 240 1.3e-12 Methyltransferase domain
Methyltransf_31 PF13847 130 - 244 9.1e-11 Methyltransferase domain
Methyltransf_25 PF13649 134 - 230 3e-17 Methyltransferase domain
Methyltransf_11 PF08241 135 - 234 8.3e-22 Methyltransferase domain
Methyltransf_12 PF08242 135 - 231 2e-11 Methyltransferase domain
Ubie_methyltran PF01209 150 - 237 6.8e-10 ubiE/COQ5 methyltransferase family
Methyltransf_8 PF05148 192 - 236 5.1e-06 Hypothetical methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009895)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69520 AT1G69520 AT1G69523 AT1G69526 AT1G69526 AT1G69526
fragaria_vesca FvH4_4g31120 FvH4_4g31120
malus_domestica MD16G1070300.v1.1
prunus_persica Prupe.1G276900_v2.0.a1 Prupe.1G276900_v2.0.a1
pyrus_communis pycom16g06120
rosa_chinensis RchiOBHm_Chr4g0439511
rosa_laevigata RLG00000006262
rosa_multiflora Rmu_co8194290.1_g000001
rosa_roxburghii Rroxscaffold_5G00380430
rosa_samantha Rh4AG370300 Rh4BG382200 Rh4CG396800 Rh4DG376900
rosa_wichuraiana Rw4G031570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 884
AasI GACNNNNNNGTC 1 cut(s) 865
AccBSI CCGCTC 1 cut(s) 135
AciI CCGC 5 cut(s) 98, 135, 150, 350, 515
AclWI GGATC 4 cut(s) 473, 486, 755, 822
AcoI YGGCCR 3 cut(s) 99, 249, 516
AcvI CACGTG 1 cut(s) 44
AcyI GRCGYC 1 cut(s) 843
AfaI GTAC 2 cut(s) 236, 874
AfiI CCNNNNNNNGG 1 cut(s) 258
AgsI TTSAA 2 cut(s) 316, 382
AjnI CCWGG 1 cut(s) 682
AluBI AGCT 4 cut(s) 367, 392, 850, 908
AluI AGCT 4 cut(s) 367, 392, 850, 908
Alw21I GWGCWC 1 cut(s) 369
Alw26I GTCTC 1 cut(s) 673
AlwI GGATC 4 cut(s) 473, 486, 755, 822
Ama87I CYCGRG 3 cut(s) 75, 110, 801
AoxI GGCC 4 cut(s) 99, 113, 249, 516
ApeKI GCWGC 4 cut(s) 173, 512, 524, 716
ArsI GACNNNNNNTTYG 2 cut(s) 162, 194
AspS9I GGNCC 2 cut(s) 113, 425
AsuC2I CCSGG 3 cut(s) 103, 802, 803
AsuHPI GGTGA 2 cut(s) 260, 790
AvaI CYCGRG 3 cut(s) 75, 110, 801
AvaII GGWCC 1 cut(s) 425
BaeI ACNNNNGTAYC 2 cut(s) 430, 463
BamHI GGATCC 1 cut(s) 478
BanII GRGCYC 1 cut(s) 369
BbrPI CACGTG 1 cut(s) 44
BbsI GAAGAC 2 cut(s) 163, 345
Bbv12I GWGCWC 1 cut(s) 369
BbvI GCAGC 4 cut(s) 185, 499, 511, 703
BccI CCATC 4 cut(s) 449, 487, 719, 779
BceAI ACGGC 2 cut(s) 264, 503
BciT130I CCWGG 1 cut(s) 684
BcnI CCSGG 3 cut(s) 103, 802, 803
BcoDI GTCTC 1 cut(s) 673
BisI GCNGC 7 cut(s) 99, 136, 174, 513, 516, 525, 717
BlpI GCTNAGC 1 cut(s) 393
BlsI GCNGC 7 cut(s) 100, 137, 175, 514, 517, 526, 718
Bme1390I CCNGG 4 cut(s) 103, 684, 802, 803
Bme18I GGWCC 1 cut(s) 425
BmeT110I CYCGRG 3 cut(s) 75, 110, 801
BmgT120I GGNCC 2 cut(s) 113, 425
BmiI GGNNCC 3 cut(s) 480, 616, 814
BmrFI CCNGG 4 cut(s) 103, 684, 802, 803
BmrI ACTGGG 1 cut(s) 94
BmsI GCATC 2 cut(s) 580, 592
BmuI ACTGGG 1 cut(s) 94
BoxI GACNNNNGTC 1 cut(s) 77
BpiI GAAGAC 2 cut(s) 163, 345
Bpu10I CCTNAGC 1 cut(s) 507
Bpu1102I GCTNAGC 1 cut(s) 393
BpuMI CCSGG 3 cut(s) 103, 802, 803
BsaAI YACGTR 1 cut(s) 44
BsaHI GRCGYC 1 cut(s) 843
BsaI GGTCTC 1 cut(s) 673
BsaJI CCNNGG 2 cut(s) 519, 801
BsaWI WCCGGW 1 cut(s) 280
Bsc4I CCNNNNNNNGG 1 cut(s) 258
Bse118I RCCGGY 1 cut(s) 320
Bse1I ACTGG 4 cut(s) 89, 290, 427, 870
BseBI CCWGG 1 cut(s) 684
BseDI CCNNGG 2 cut(s) 519, 801
BseGI GGATG 1 cut(s) 26
BseLI CCNNNNNNNGG 1 cut(s) 258
BseMII CTCAG 4 cut(s) 384, 668, 751, 852
BseNI ACTGG 4 cut(s) 89, 290, 427, 870
BseRI GAGGAG 1 cut(s) 264
BseX3I CGGCCG 2 cut(s) 99, 516
BseXI GCAGC 4 cut(s) 185, 499, 511, 703
Bsh1285I CGRYCG 3 cut(s) 102, 281, 519
BshFI GGCC 4 cut(s) 101, 115, 251, 518
BsiEI CGRYCG 3 cut(s) 102, 281, 519
BsiHKAI GWGCWC 1 cut(s) 369
BsiHKCI CYCGRG 3 cut(s) 75, 110, 801
BsiSI CCGG 7 cut(s) 30, 102, 116, 281, 321, 802, 831
BslFI GGGAC 2 cut(s) 433, 742
BslI CCNNNNNNNGG 1 cut(s) 258
BsmAI GTCTC 1 cut(s) 673
BsmFI GGGAC 2 cut(s) 433, 742
BsnI GGCC 4 cut(s) 101, 115, 251, 518
Bso31I GGTCTC 1 cut(s) 673
BsoBI CYCGRG 3 cut(s) 75, 110, 801
Bsp1286I GDGCHC 1 cut(s) 369
Bsp143I GATC 4 cut(s) 3, 478, 760, 827
Bsp1720I GCTNAGC 1 cut(s) 393
BspACI CCGC 5 cut(s) 98, 135, 150, 350, 515
BspANI GGCC 4 cut(s) 101, 115, 251, 518
BspCNI CTCAG 4 cut(s) 385, 669, 750, 851
BspLI GGNNCC 3 cut(s) 480, 616, 814
BspPI GGATC 4 cut(s) 473, 486, 755, 822
BspTNI GGTCTC 1 cut(s) 673
BsrBI CCGCTC 1 cut(s) 135
BsrFI RCCGGY 1 cut(s) 320
BsrI ACTGG 4 cut(s) 89, 290, 427, 870
BssAI RCCGGY 1 cut(s) 320
BssECI CCNNGG 2 cut(s) 519, 801
BssMI GATC 4 cut(s) 3, 478, 760, 827
BssNI GRCGYC 1 cut(s) 843
Bst2UI CCWGG 1 cut(s) 684
Bst4CI ACNGT 1 cut(s) 14
Bst6I CTCTTC 2 cut(s) 26, 288
BstACI GRCGYC 1 cut(s) 843
BstBAI YACGTR 1 cut(s) 44
BstC8I GCNNGC 3 cut(s) 326, 780, 910
BstDEI CTNAG 8 cut(s) 375, 393, 434, 507, 677, 737, 838, 851
BstDSI CCRYGG 1 cut(s) 519
BstF5I GGATG 1 cut(s) 26
BstKTI GATC 4 cut(s) 6, 481, 763, 830
BstMAI GTCTC 1 cut(s) 673
BstMBI GATC 4 cut(s) 3, 478, 760, 827
BstMCI CGRYCG 3 cut(s) 102, 281, 519
BstMWI GCNNNNNNNGC 5 cut(s) 512, 521, 524, 775, 840
BstNI CCWGG 1 cut(s) 684
BstNSI RCATGY 2 cut(s) 328, 713
BstPAI GACNNNNGTC 1 cut(s) 77
BstSCI CCNGG 4 cut(s) 101, 682, 800, 801
BstV1I GCAGC 4 cut(s) 185, 499, 511, 703
BstV2I GAAGAC 2 cut(s) 163, 345
BstX2I RGATCY 2 cut(s) 478, 827
BstYI RGATCY 2 cut(s) 478, 827
BstZI CGGCCG 2 cut(s) 99, 516
BsuRI GGCC 4 cut(s) 101, 115, 251, 518
BtgI CCRYGG 1 cut(s) 519
BtsCI GGATG 1 cut(s) 26
Cac8I GCNNGC 3 cut(s) 326, 780, 910
Cfr10I RCCGGY 1 cut(s) 320
Cfr13I GGNCC 2 cut(s) 113, 425
Cfr9I CCCGGG 1 cut(s) 801
CseI GACGC 3 cut(s) 168, 294, 832
CsiI ACCWGGT 1 cut(s) 682
Csp6I GTAC 2 cut(s) 235, 873
CviAII CATG 2 cut(s) 325, 710
CviQI GTAC 2 cut(s) 235, 873
DdeI CTNAG 8 cut(s) 375, 393, 434, 507, 677, 737, 838, 851
DpnI GATC 4 cut(s) 5, 480, 762, 829
DpnII GATC 4 cut(s) 3, 478, 760, 827
DrdI GACNNNNNNGTC 1 cut(s) 865
DseDI GACNNNNNNGTC 1 cut(s) 865
EaeI YGGCCR 3 cut(s) 99, 249, 516
EagI CGGCCG 2 cut(s) 99, 516
Eam1104I CTCTTC 2 cut(s) 26, 288
EarI CTCTTC 2 cut(s) 26, 288
Ecl136II GAGCTC 1 cut(s) 367
EclXI CGGCCG 2 cut(s) 99, 516
Eco24I GRGCYC 1 cut(s) 369
Eco31I GGTCTC 1 cut(s) 673
Eco47I GGWCC 1 cut(s) 425
Eco52I CGGCCG 2 cut(s) 99, 516
Eco53kI GAGCTC 1 cut(s) 367
Eco72I CACGTG 1 cut(s) 44
Eco88I CYCGRG 3 cut(s) 75, 110, 801
EcoICRI GAGCTC 1 cut(s) 367
EcoRII CCWGG 1 cut(s) 682
EcoT38I GRGCYC 1 cut(s) 369
FaeI CATG 2 cut(s) 328, 713
FaqI GGGAC 2 cut(s) 433, 742
FatI CATG 2 cut(s) 324, 709
FauI CCCGC 1 cut(s) 343
Fnu4HI GCNGC 7 cut(s) 99, 136, 174, 513, 516, 525, 717
FokI GGATG 1 cut(s) 13
FriOI GRGCYC 1 cut(s) 369
Fsp4HI GCNGC 7 cut(s) 99, 136, 174, 513, 516, 525, 717
GluI GCNGC 7 cut(s) 99, 136, 174, 513, 516, 525, 717
HaeIII GGCC 4 cut(s) 101, 115, 251, 518
HapII CCGG 7 cut(s) 30, 102, 116, 281, 321, 802, 831
HgaI GACGC 3 cut(s) 168, 294, 832
Hin1I GRCGYC 1 cut(s) 843
Hin1II CATG 2 cut(s) 328, 713
HincII GTYRAC 2 cut(s) 82, 646
HindII GTYRAC 2 cut(s) 82, 646
HinfI GANTC 4 cut(s) 78, 119, 652, 690
HpaII CCGG 7 cut(s) 30, 102, 116, 281, 321, 802, 831
HphI GGTGA 2 cut(s) 260, 790
Hpy166II GTNNAC 4 cut(s) 82, 268, 646, 689
Hpy188I TCNGA 6 cut(s) 72, 132, 364, 464, 543, 740
Hpy188III TCNNGA 2 cut(s) 758, 825
Hpy8I GTNNAC 4 cut(s) 82, 268, 646, 689
Hpy99I CGWCG 4 cut(s) 221, 242, 310, 848
HpyAV CCTTC 3 cut(s) 83, 376, 548
HpyCH4III ACNGT 1 cut(s) 14
HpyCH4IV ACGT 2 cut(s) 43, 254
HpyCH4V TGCA 5 cut(s) 328, 605, 719, 769, 912
HpyF10VI GCNNNNNNNGC 5 cut(s) 512, 521, 524, 775, 840
HpyF3I CTNAG 8 cut(s) 375, 393, 434, 507, 677, 737, 838, 851
HpySE526I ACGT 2 cut(s) 43, 254
Hsp92I GRCGYC 1 cut(s) 843
Hsp92II CATG 2 cut(s) 328, 713
Kzo9I GATC 4 cut(s) 3, 478, 760, 827
LmnI GCTCC 2 cut(s) 364, 706
Lsp1109I GCAGC 4 cut(s) 185, 499, 511, 703
LweI GCATC 2 cut(s) 580, 592
MabI ACCWGGT 1 cut(s) 682
MaeII ACGT 2 cut(s) 43, 254
MaeIII GTNAC 4 cut(s) 34, 105, 178, 353
MalI GATC 4 cut(s) 5, 480, 762, 829
MbiI CCGCTC 1 cut(s) 135
MboI GATC 4 cut(s) 3, 478, 760, 827
MboII GAAGA 8 cut(s) 13, 51, 54, 57, 165, 168, 305, 350
MflI RGATCY 2 cut(s) 478, 827
MhlI GDGCHC 1 cut(s) 369
MluCI AATT 3 cut(s) 198, 408, 879
MlyI GAGTC 4 cut(s) 87, 113, 646, 684
MmeI TCCRAC 4 cut(s) 95, 342, 384, 592
MseI TTAA 1 cut(s) 666
MslI CAYNNNNRTG 1 cut(s) 323
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 7 cut(s) 30, 102, 116, 281, 321, 802, 831
MspR9I CCNGG 4 cut(s) 103, 684, 802, 803
MvaI CCWGG 1 cut(s) 684
MwoI GCNNNNNNNGC 5 cut(s) 512, 521, 524, 775, 840
NciI CCSGG 3 cut(s) 103, 802, 803
NdeII GATC 4 cut(s) 3, 478, 760, 827
NlaIII CATG 2 cut(s) 328, 713
NlaIV GGNNCC 3 cut(s) 480, 616, 814
NmuCI GTSAC 2 cut(s) 105, 178
NspI RCATGY 2 cut(s) 328, 713
PaeI GCATGC 1 cut(s) 328
PcsI WCGNNNNNNNCGW 1 cut(s) 128
PkrI GCNGC 7 cut(s) 100, 137, 175, 514, 517, 526, 718
PleI GAGTC 4 cut(s) 86, 113, 646, 684
PmaCI CACGTG 1 cut(s) 44
PmlI CACGTG 1 cut(s) 44
PpsI GAGTC 4 cut(s) 86, 113, 646, 684
Ppu21I YACGTR 1 cut(s) 44
PshAI GACNNNNGTC 1 cut(s) 77
PsiI TTATAA 1 cut(s) 884
Psp124BI GAGCTC 1 cut(s) 369
Psp6I CCWGG 1 cut(s) 682
PspCI CACGTG 1 cut(s) 44
PspGI CCWGG 1 cut(s) 682
PspN4I GGNNCC 3 cut(s) 480, 616, 814
PspPI GGNCC 2 cut(s) 113, 425
PsuI RGATCY 2 cut(s) 478, 827
RsaI GTAC 2 cut(s) 236, 874
RsaNI GTAC 2 cut(s) 235, 873
RseI CAYNNNNRTG 1 cut(s) 323
SacI GAGCTC 1 cut(s) 369
SaqAI TTAA 1 cut(s) 666
SatI GCNGC 7 cut(s) 99, 136, 174, 513, 516, 525, 717
Sau3AI GATC 4 cut(s) 3, 478, 760, 827
Sau96I GGNCC 2 cut(s) 113, 425
SchI GAGTC 4 cut(s) 87, 113, 646, 684
ScrFI CCNGG 4 cut(s) 103, 684, 802, 803
SduI GDGCHC 1 cut(s) 369
SexAI ACCWGGT 1 cut(s) 682
SfaNI GCATC 2 cut(s) 580, 592
SinI GGWCC 1 cut(s) 425
SmaI CCCGGG 1 cut(s) 803
SmiMI CAYNNNNRTG 1 cut(s) 323
SphI GCATGC 1 cut(s) 328
Sse9I AATT 3 cut(s) 198, 408, 879
SsiI CCGC 5 cut(s) 98, 135, 150, 350, 515
SstI GAGCTC 1 cut(s) 369
StyD4I CCNGG 4 cut(s) 101, 682, 800, 801
TaaI ACNGT 1 cut(s) 14
TaiI ACGT 2 cut(s) 46, 257
TaqI TCGA 3 cut(s) 168, 277, 846
TasI AATT 3 cut(s) 198, 408, 879
TauI GCSGC 3 cut(s) 101, 138, 518
Tru1I TTAA 1 cut(s) 666
Tru9I TTAA 1 cut(s) 666
TseFI GTSAC 2 cut(s) 105, 178
TseI GCWGC 4 cut(s) 173, 512, 524, 716
Tsp45I GTSAC 2 cut(s) 105, 178
TspDTI ATGAA 3 cut(s) 154, 174, 306
TspGWI ACGGA 2 cut(s) 205, 622
TspMI CCCGGG 1 cut(s) 801
VpaK11BI GGWCC 1 cut(s) 425
XceI RCATGY 2 cut(s) 328, 713
XmaI CCCGGG 1 cut(s) 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.