FvH4_4g31120

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009895
Physical Location & Seq
Forward (+)
30399482 .. 30401323
1842 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g31120.t2

Sequence Viewer

Length: 894 bp
ATGATCCTTTACAGTCCTCTCTTCTCATCACCCATTAGGCCACGTGGCATCACTTCTTCTTCTCCGACCCCCCGAGTCAACTCGGATGCTTCGGACTCGTTCTGTCCGAGCGGCTTATGTTCTTGCGGCAGAAGACGCCTCATCGAAGCAGCAGTCGCCACTTCTTTATTCCCGGTTTTCCCTTCCACCTCCGTCGCTTCCAAATTGCAGTCCGATGATTATACGGCCACATTGAGGAAAGTACACCCTCCTAGACCAGACTGGTACGAAGAGTTGTATGCGTCAGTGTTGAACAAGGGCATGCGAACTTATGAAGACGAGATTGCAGGTTGCAAGTCGGAGCTGTTTGCTAGGTTGAAGGGAAATGCTGAGCGAGTATTGGAAATTGGTATAGGGACTGGTCCGAATTTAAGATACTACAGTGCCGATGATGGTGTCCGAGTTTTTGGTGTGGATCCTAATAGAAAGATGGAAAGATACGCTCAGGCTGCGGCCGTGGCTGCTGGTTTGCCCCTTTCGAACTTCGAGTTTATACAAGCAGTTGGGGAGGCCATACCATTAGATGATGCTTCTGTTGATGCAGTTGTTGGAACCCTAGTATTGTGTTCTGTAAAAGATGTCGATAAGACTCTAAAGGAGATTAAGAGAGTGCTGAGACCAGGTGGACTCTACTTGTTTGTGGAGCATGTGGCTGCAAAAGATGGGACACCCCTCAGATTTATACAGAGTGTTCTTGATCCCTTGCAACAAACTCTTGCCGATGGATGTCATCTCACCCGGGAAACAGGAACCAATATATCACGATCCGGCTTCTCAGGCGTTGAGCTTAGCATGACCTCTTTGTCCAGTGCCTCAATTATAAACCCTCAAATCTATGGAGTAGCTAGCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

298

Amino Acids

32.09

Weight (kDa)

6.83

Isoelectric Point (pI)

42.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 114 - 230 3.7e-13 Methyltransferase domain
Methyltransf_31 PF13847 123 - 233 4.4e-08 Methyltransferase domain
Ubie_methyltran PF01209 125 - 226 9.5e-06 ubiE/COQ5 methyltransferase family
Methyltransf_25 PF13649 126 - 219 5.8e-14 Methyltransferase domain
Methyltransf_11 PF08241 127 - 223 5.4e-18 Methyltransferase domain
Methyltransf_12 PF08242 127 - 220 1.3e-11 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009895)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69520 AT1G69520 AT1G69523 AT1G69526 AT1G69526 AT1G69526
fragaria_vesca FvH4_4g31120 FvH4_4g31120
malus_domestica MD16G1070300.v1.1
prunus_persica Prupe.1G276900_v2.0.a1 Prupe.1G276900_v2.0.a1
pyrus_communis pycom16g06120
rosa_chinensis RchiOBHm_Chr4g0439511
rosa_laevigata RLG00000006262
rosa_multiflora Rmu_co8194290.1_g000001
rosa_roxburghii Rroxscaffold_5G00380430
rosa_samantha Rh4AG370300 Rh4BG382200 Rh4CG396800 Rh4DG376900
rosa_wichuraiana Rw4G031570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 860
AasI GACNNNNNNGTC 1 cut(s) 841
Acc36I ACCTGC 1 cut(s) 317
AccBSI CCGCTC 1 cut(s) 111
AciI CCGC 3 cut(s) 111, 126, 491
AclWI GGATC 4 cut(s) 449, 462, 731, 798
AcoI YGGCCR 2 cut(s) 225, 492
AcsI RAATTY 1 cut(s) 406
AcvI CACGTG 1 cut(s) 44
AcyI GRCGYC 1 cut(s) 136
AfaI GTAC 2 cut(s) 243, 266
AfiI CCNNNNNNNGG 1 cut(s) 234
AgsI TTSAA 2 cut(s) 292, 358
AjnI CCWGG 1 cut(s) 658
AluBI AGCT 3 cut(s) 343, 826, 884
AluI AGCT 3 cut(s) 343, 826, 884
Alw26I GTCTC 1 cut(s) 649
AlwI GGATC 4 cut(s) 449, 462, 731, 798
Ama87I CYCGRG 2 cut(s) 72, 777
AoxI GGCC 4 cut(s) 38, 225, 492, 549
ApeKI GCWGC 4 cut(s) 149, 488, 500, 692
ApoI RAATTY 1 cut(s) 406
ArsI GACNNNNNNTTYG 2 cut(s) 138, 170
AspS9I GGNCC 1 cut(s) 401
AsuC2I CCSGG 3 cut(s) 173, 778, 779
AsuHPI GGTGA 2 cut(s) 21, 766
AsuII TTCGAA 1 cut(s) 518
AsuNHI GCTAGC 1 cut(s) 884
AvaI CYCGRG 2 cut(s) 72, 777
AvaII GGWCC 1 cut(s) 401
BaeI ACNNNNGTAYC 2 cut(s) 406, 439
BamHI GGATCC 1 cut(s) 454
BbrPI CACGTG 1 cut(s) 44
BbsI GAAGAC 2 cut(s) 139, 321
BbvI GCAGC 4 cut(s) 161, 475, 487, 679
BccI CCATC 4 cut(s) 425, 463, 695, 755
BceAI ACGGC 2 cut(s) 240, 479
BciT130I CCWGG 1 cut(s) 660
BcnI CCSGG 3 cut(s) 173, 778, 779
BcoDI GTCTC 1 cut(s) 649
BfaI CTAG 4 cut(s) 252, 351, 596, 885
BfmI CTRYAG 1 cut(s) 418
BfuAI ACCTGC 1 cut(s) 317
BisI GCNGC 7 cut(s) 112, 127, 150, 489, 492, 501, 693
BlpI GCTNAGC 2 cut(s) 369, 827
BlsI GCNGC 7 cut(s) 113, 128, 151, 490, 493, 502, 694
Bme1390I CCNGG 4 cut(s) 173, 660, 778, 779
Bme18I GGWCC 1 cut(s) 401
BmeT110I CYCGRG 2 cut(s) 72, 777
BmgT120I GGNCC 1 cut(s) 401
BmiI GGNNCC 3 cut(s) 456, 592, 790
BmrFI CCNGG 4 cut(s) 173, 660, 778, 779
BmsI GCATC 4 cut(s) 57, 76, 556, 568
BmtI GCTAGC 1 cut(s) 888
BpiI GAAGAC 2 cut(s) 139, 321
Bpu10I CCTNAGC 1 cut(s) 483
Bpu1102I GCTNAGC 2 cut(s) 369, 827
Bpu14I TTCGAA 1 cut(s) 518
BpuMI CCSGG 3 cut(s) 173, 778, 779
BsaAI YACGTR 1 cut(s) 44
BsaHI GRCGYC 1 cut(s) 136
BsaI GGTCTC 1 cut(s) 649
BsaJI CCNNGG 2 cut(s) 495, 777
Bsc4I CCNNNNNNNGG 1 cut(s) 234
Bse1I ACTGG 3 cut(s) 266, 403, 846
BseBI CCWGG 1 cut(s) 660
BseDI CCNNGG 2 cut(s) 495, 777
BseGI GGATG 2 cut(s) 91, 770
BseLI CCNNNNNNNGG 1 cut(s) 234
BseMII CTCAG 5 cut(s) 360, 497, 644, 727, 828
BseNI ACTGG 3 cut(s) 266, 403, 846
BseX3I CGGCCG 1 cut(s) 492
BseXI GCAGC 4 cut(s) 161, 475, 487, 679
Bsh1285I CGRYCG 1 cut(s) 495
BshFI GGCC 4 cut(s) 40, 227, 494, 551
BsiEI CGRYCG 1 cut(s) 495
BsiHKCI CYCGRG 2 cut(s) 72, 777
BsiSI CCGG 3 cut(s) 173, 778, 807
BslFI GGGAC 2 cut(s) 409, 718
BslI CCNNNNNNNGG 1 cut(s) 234
BsmAI GTCTC 1 cut(s) 649
BsmFI GGGAC 2 cut(s) 409, 718
BsnI GGCC 4 cut(s) 40, 227, 494, 551
Bso31I GGTCTC 1 cut(s) 649
BsoBI CYCGRG 2 cut(s) 72, 777
Bsp119I TTCGAA 1 cut(s) 518
Bsp143I GATC 4 cut(s) 3, 454, 736, 803
Bsp1720I GCTNAGC 2 cut(s) 369, 827
BspACI CCGC 3 cut(s) 111, 126, 491
BspANI GGCC 4 cut(s) 40, 227, 494, 551
BspCNI CTCAG 5 cut(s) 361, 496, 645, 726, 827
BspLI GGNNCC 3 cut(s) 456, 592, 790
BspMI ACCTGC 1 cut(s) 317
BspOI GCTAGC 1 cut(s) 888
BspPI GGATC 4 cut(s) 449, 462, 731, 798
BspT104I TTCGAA 1 cut(s) 518
BspTNI GGTCTC 1 cut(s) 649
BsrBI CCGCTC 1 cut(s) 111
BsrI ACTGG 3 cut(s) 266, 403, 846
BssECI CCNNGG 2 cut(s) 495, 777
BssMI GATC 4 cut(s) 3, 454, 736, 803
BssNI GRCGYC 1 cut(s) 136
Bst2UI CCWGG 1 cut(s) 660
Bst4CI ACNGT 2 cut(s) 14, 422
Bst6I CTCTTC 2 cut(s) 26, 264
BstACI GRCGYC 1 cut(s) 136
BstBAI YACGTR 1 cut(s) 44
BstBI TTCGAA 1 cut(s) 518
BstC8I GCNNGC 2 cut(s) 302, 886
BstDEI CTNAG 6 cut(s) 369, 483, 653, 713, 814, 827
BstDSI CCRYGG 1 cut(s) 495
BstF5I GGATG 2 cut(s) 91, 770
BstKTI GATC 4 cut(s) 6, 457, 739, 806
BstMAI GTCTC 1 cut(s) 649
BstMBI GATC 4 cut(s) 3, 454, 736, 803
BstMCI CGRYCG 1 cut(s) 495
BstMWI GCNNNNNNNGC 6 cut(s) 135, 155, 488, 497, 500, 816
BstNI CCWGG 1 cut(s) 660
BstNSI RCATGY 2 cut(s) 304, 689
BstSCI CCNGG 4 cut(s) 171, 658, 776, 777
BstSFI CTRYAG 1 cut(s) 418
BstV1I GCAGC 4 cut(s) 161, 475, 487, 679
BstV2I GAAGAC 2 cut(s) 139, 321
BstX2I RGATCY 1 cut(s) 454
BstYI RGATCY 1 cut(s) 454
BstZI CGGCCG 1 cut(s) 492
BsuRI GGCC 4 cut(s) 40, 227, 494, 551
BtgI CCRYGG 1 cut(s) 495
BtsCI GGATG 2 cut(s) 91, 770
BtsIMutI CAGTG 3 cut(s) 291, 427, 853
BveI ACCTGC 1 cut(s) 317
Cac8I GCNNGC 2 cut(s) 302, 886
Cfr13I GGNCC 1 cut(s) 401
Cfr9I CCCGGG 1 cut(s) 777
CseI GACGC 2 cut(s) 144, 270
CsiI ACCWGGT 1 cut(s) 658
Csp6I GTAC 2 cut(s) 242, 265
CviAII CATG 3 cut(s) 301, 686, 832
CviQI GTAC 2 cut(s) 242, 265
DdeI CTNAG 6 cut(s) 369, 483, 653, 713, 814, 827
DpnI GATC 4 cut(s) 5, 456, 738, 805
DpnII GATC 4 cut(s) 3, 454, 736, 803
DrdI GACNNNNNNGTC 1 cut(s) 841
DseDI GACNNNNNNGTC 1 cut(s) 841
EaeI YGGCCR 2 cut(s) 225, 492
EagI CGGCCG 1 cut(s) 492
Eam1104I CTCTTC 2 cut(s) 26, 264
EarI CTCTTC 2 cut(s) 26, 264
EclXI CGGCCG 1 cut(s) 492
Eco31I GGTCTC 1 cut(s) 649
Eco47I GGWCC 1 cut(s) 401
Eco52I CGGCCG 1 cut(s) 492
Eco72I CACGTG 1 cut(s) 44
Eco88I CYCGRG 2 cut(s) 72, 777
EcoRII CCWGG 1 cut(s) 658
FaeI CATG 3 cut(s) 304, 689, 835
FaqI GGGAC 2 cut(s) 409, 718
FatI CATG 3 cut(s) 300, 685, 831
Fnu4HI GCNGC 7 cut(s) 112, 127, 150, 489, 492, 501, 693
FokI GGATG 2 cut(s) 98, 777
Fsp4HI GCNGC 7 cut(s) 112, 127, 150, 489, 492, 501, 693
FspBI CTAG 4 cut(s) 252, 351, 596, 885
GluI GCNGC 7 cut(s) 112, 127, 150, 489, 492, 501, 693
HaeIII GGCC 4 cut(s) 40, 227, 494, 551
HapII CCGG 3 cut(s) 173, 778, 807
HgaI GACGC 2 cut(s) 144, 270
Hin1I GRCGYC 1 cut(s) 136
Hin1II CATG 3 cut(s) 304, 689, 835
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HinfI GANTC 4 cut(s) 75, 95, 628, 666
HpaII CCGG 3 cut(s) 173, 778, 807
HphI GGTGA 2 cut(s) 21, 766
Hpy166II GTNNAC 3 cut(s) 79, 244, 665
Hpy188I TCNGA 9 cut(s) 66, 85, 94, 108, 214, 340, 405, 440, 716
Hpy188III TCNNGA 2 cut(s) 734, 801
Hpy8I GTNNAC 3 cut(s) 79, 244, 665
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 2 cut(s) 192, 352
HpyCH4III ACNGT 2 cut(s) 14, 422
HpyCH4IV ACGT 1 cut(s) 43
HpyCH4V TGCA 6 cut(s) 208, 326, 333, 581, 695, 745
HpyF10VI GCNNNNNNNGC 6 cut(s) 135, 155, 488, 497, 500, 816
HpyF3I CTNAG 6 cut(s) 369, 483, 653, 713, 814, 827
HpySE526I ACGT 1 cut(s) 43
Hsp92I GRCGYC 1 cut(s) 136
Hsp92II CATG 3 cut(s) 304, 689, 835
Kzo9I GATC 4 cut(s) 3, 454, 736, 803
LmnI GCTCC 2 cut(s) 340, 682
Lsp1109I GCAGC 4 cut(s) 161, 475, 487, 679
LweI GCATC 4 cut(s) 57, 76, 556, 568
MabI ACCWGGT 1 cut(s) 658
MaeI CTAG 4 cut(s) 252, 351, 596, 885
MaeII ACGT 1 cut(s) 43
MalI GATC 4 cut(s) 5, 456, 738, 805
MbiI CCGCTC 1 cut(s) 111
MboI GATC 4 cut(s) 3, 454, 736, 803
MboII GAAGA 6 cut(s) 13, 48, 51, 144, 281, 326
MflI RGATCY 1 cut(s) 454
MluCI AATT 4 cut(s) 203, 384, 406, 855
MlyI GAGTC 4 cut(s) 84, 89, 622, 660
MmeI TCCRAC 3 cut(s) 89, 318, 568
MseI TTAA 2 cut(s) 410, 642
MspI CCGG 3 cut(s) 173, 778, 807
MspR9I CCNGG 4 cut(s) 173, 660, 778, 779
MvaI CCWGG 1 cut(s) 660
MwoI GCNNNNNNNGC 6 cut(s) 135, 155, 488, 497, 500, 816
NciI CCSGG 3 cut(s) 173, 778, 779
NdeII GATC 4 cut(s) 3, 454, 736, 803
NheI GCTAGC 1 cut(s) 884
NlaIII CATG 3 cut(s) 304, 689, 835
NlaIV GGNNCC 3 cut(s) 456, 592, 790
NspI RCATGY 2 cut(s) 304, 689
NspV TTCGAA 1 cut(s) 518
PaeI GCATGC 1 cut(s) 304
PcsI WCGNNNNNNNCGW 1 cut(s) 104
PkrI GCNGC 7 cut(s) 113, 128, 151, 490, 493, 502, 694
PleI GAGTC 4 cut(s) 83, 89, 622, 660
PmaCI CACGTG 1 cut(s) 44
PmlI CACGTG 1 cut(s) 44
PpsI GAGTC 4 cut(s) 83, 89, 622, 660
Ppu21I YACGTR 1 cut(s) 44
PsiI TTATAA 1 cut(s) 860
Psp6I CCWGG 1 cut(s) 658
PspCI CACGTG 1 cut(s) 44
PspGI CCWGG 1 cut(s) 658
PspN4I GGNNCC 3 cut(s) 456, 592, 790
PspPI GGNCC 1 cut(s) 401
PsuI RGATCY 1 cut(s) 454
RsaI GTAC 2 cut(s) 243, 266
RsaNI GTAC 2 cut(s) 242, 265
SaqAI TTAA 2 cut(s) 410, 642
SatI GCNGC 7 cut(s) 112, 127, 150, 489, 492, 501, 693
Sau3AI GATC 4 cut(s) 3, 454, 736, 803
Sau96I GGNCC 1 cut(s) 401
SchI GAGTC 4 cut(s) 84, 89, 622, 660
ScrFI CCNGG 4 cut(s) 173, 660, 778, 779
SetI ASST 9 cut(s) 46, 191, 331, 345, 356, 664, 828, 839, 886
SexAI ACCWGGT 1 cut(s) 658
SfaNI GCATC 4 cut(s) 57, 76, 556, 568
SfcI CTRYAG 1 cut(s) 418
SfuI TTCGAA 1 cut(s) 518
SinI GGWCC 1 cut(s) 401
SmaI CCCGGG 1 cut(s) 779
SphI GCATGC 1 cut(s) 304
Sse9I AATT 4 cut(s) 203, 384, 406, 855
SsiI CCGC 3 cut(s) 111, 126, 491
SspMI CTAG 4 cut(s) 252, 351, 596, 885
StyD4I CCNGG 4 cut(s) 171, 658, 776, 777
TaaI ACNGT 2 cut(s) 14, 422
TaiI ACGT 1 cut(s) 46
TaqI TCGA 4 cut(s) 144, 518, 525, 621
TasI AATT 4 cut(s) 203, 384, 406, 855
TatI WGTACW 1 cut(s) 241
TauI GCSGC 3 cut(s) 114, 129, 494
Tru1I TTAA 2 cut(s) 410, 642
Tru9I TTAA 2 cut(s) 410, 642
TscAI CASTG 3 cut(s) 291, 427, 853
TseI GCWGC 4 cut(s) 149, 488, 500, 692
TspDTI ATGAA 1 cut(s) 327
TspGWI ACGGA 1 cut(s) 181
TspMI CCCGGG 1 cut(s) 777
TspRI CASTG 3 cut(s) 291, 427, 853
VpaK11BI GGWCC 1 cut(s) 401
XapI RAATTY 1 cut(s) 406
XceI RCATGY 2 cut(s) 304, 689
XmaI CCCGGG 1 cut(s) 777
XspI CTAG 4 cut(s) 252, 351, 596, 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.