Rh4DG376900

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0009895
Physical Location & Seq
Forward (+)
59447948 .. 59449497
1550 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG376900.1

Sequence Viewer

Length: 594 bp
ATGCAATCTTACGAAGAAGAGATTGCGGGTTACAAGTCGGAGCTCTTTGCTAAGTTGAAGGGAAAAGCTGAGCAAGTGTTGGAAATTGGTATAGGGACTGGTCCTAATCTAAGATACTATGGTGCTGATGATGGTGTCCGAGTTTTCGGCGTGGATCCTAATAAAAAGATGGAAAGATATGCTAAGGCTGCGGCAGTGGCTGCTGGTCTGCCCCTTTCGAACTTTGAGTTTATACAAGCAGTTGGGGAGGCTATACCATTAGATGATGCTTCTGTTGATGCAGTTGTTGGAACCCTAGTATTGTGTTCTGTAAAAGATGTCAACAAGACTCTAAAAGAGATTAAGAGAGTGCTGAGACCAGGTGGACTCTACTTGTTTGTGGAGCATGTGGCTGCTAAAGATGGGACAGCCCTCAGATTTATACAGAGTGTTCTTGATCCTTTGCAACAAACCCTTGCCGATGGGTGTCATCTCACCCGGGAAACAGGAACCAATATATCCAGATCCGGCTTCTCAGGCGTCGAGCTTAGCATGACCTCCTTGTCCAGTACCTCAATTATAAACCCTCAAATCTATGGAATAGCTTGCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

197

Amino Acids

21.14

Weight (kDa)

5.76

Isoelectric Point (pI)

33.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 6 - 134 2.5e-13 Methyltransferase domain
Methyltransf_31 PF13847 22 - 136 1.2e-11 Methyltransferase domain
Methyltransf_25 PF13649 26 - 122 1.1e-17 Methyltransferase domain
Methyltransf_11 PF08241 27 - 126 3.1e-22 Methyltransferase domain
Methyltransf_12 PF08242 27 - 123 3.6e-12 Methyltransferase domain
Ubie_methyltran PF01209 42 - 129 1.9e-10 ubiE/COQ5 methyltransferase family
Methyltransf_8 PF05148 84 - 128 2.5e-06 Hypothetical methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009895)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69520 AT1G69520 AT1G69523 AT1G69526 AT1G69526 AT1G69526
fragaria_vesca FvH4_4g31120 FvH4_4g31120
malus_domestica MD16G1070300.v1.1
prunus_persica Prupe.1G276900_v2.0.a1 Prupe.1G276900_v2.0.a1
pyrus_communis pycom16g06120
rosa_chinensis RchiOBHm_Chr4g0439511
rosa_laevigata RLG00000006262
rosa_multiflora Rmu_co8194290.1_g000001
rosa_roxburghii Rroxscaffold_5G00380430
rosa_samantha Rh4AG370300 Rh4BG382200 Rh4CG396800 Rh4DG376900
rosa_wichuraiana Rw4G031570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 560
AasI GACNNNNNNGTC 1 cut(s) 541
AciI CCGC 2 cut(s) 26, 191
AclWI GGATC 4 cut(s) 149, 162, 431, 498
AcyI GRCGYC 1 cut(s) 519
AfaI GTAC 1 cut(s) 550
AgsI TTSAA 1 cut(s) 58
AjnI CCWGG 1 cut(s) 358
AluBI AGCT 4 cut(s) 43, 68, 526, 584
AluI AGCT 4 cut(s) 43, 68, 526, 584
Alw21I GWGCWC 1 cut(s) 45
Alw26I GTCTC 1 cut(s) 349
AlwI GGATC 4 cut(s) 149, 162, 431, 498
AlwNI CAGNNNCTG 1 cut(s) 200
Ama87I CYCGRG 1 cut(s) 477
ApeKI GCWGC 3 cut(s) 188, 200, 392
AspS9I GGNCC 1 cut(s) 101
AsuC2I CCSGG 2 cut(s) 478, 479
AsuHPI GGTGA 1 cut(s) 466
AsuII TTCGAA 1 cut(s) 218
AvaI CYCGRG 1 cut(s) 477
AvaII GGWCC 1 cut(s) 101
BaeI ACNNNNGTAYC 2 cut(s) 106, 139
BamHI GGATCC 1 cut(s) 154
BanII GRGCYC 1 cut(s) 45
Bbv12I GWGCWC 1 cut(s) 45
BbvI GCAGC 3 cut(s) 175, 187, 379
BccI CCATC 4 cut(s) 125, 163, 395, 455
BciT130I CCWGG 1 cut(s) 360
BcnI CCSGG 2 cut(s) 478, 479
BcoDI GTCTC 1 cut(s) 349
BfaI CTAG 1 cut(s) 296
BisI GCNGC 4 cut(s) 189, 192, 201, 393
BlpI GCTNAGC 2 cut(s) 69, 527
BlsI GCNGC 4 cut(s) 190, 193, 202, 394
Bme1390I CCNGG 3 cut(s) 360, 478, 479
Bme18I GGWCC 1 cut(s) 101
BmeT110I CYCGRG 1 cut(s) 477
BmgT120I GGNCC 1 cut(s) 101
BmiI GGNNCC 3 cut(s) 156, 292, 490
BmrFI CCNGG 3 cut(s) 360, 478, 479
BmsI GCATC 2 cut(s) 256, 268
Bpu10I CCTNAGC 1 cut(s) 183
Bpu1102I GCTNAGC 2 cut(s) 69, 527
Bpu14I TTCGAA 1 cut(s) 218
BpuMI CCSGG 2 cut(s) 478, 479
BsaHI GRCGYC 1 cut(s) 519
BsaI GGTCTC 1 cut(s) 349
BsaJI CCNNGG 1 cut(s) 477
Bse1I ACTGG 2 cut(s) 103, 546
BseBI CCWGG 1 cut(s) 360
BseDI CCNNGG 1 cut(s) 477
BseMII CTCAG 4 cut(s) 60, 344, 427, 528
BseNI ACTGG 2 cut(s) 103, 546
BseXI GCAGC 3 cut(s) 175, 187, 379
BsiHKAI GWGCWC 1 cut(s) 45
BsiHKCI CYCGRG 1 cut(s) 477
BsiSI CCGG 2 cut(s) 478, 507
BslFI GGGAC 2 cut(s) 109, 418
BsmAI GTCTC 1 cut(s) 349
BsmFI GGGAC 2 cut(s) 109, 418
Bso31I GGTCTC 1 cut(s) 349
BsoBI CYCGRG 1 cut(s) 477
Bsp119I TTCGAA 1 cut(s) 218
Bsp1286I GDGCHC 1 cut(s) 45
Bsp143I GATC 3 cut(s) 154, 436, 503
Bsp1720I GCTNAGC 2 cut(s) 69, 527
BspACI CCGC 2 cut(s) 26, 191
BspCNI CTCAG 4 cut(s) 61, 345, 426, 527
BspLI GGNNCC 3 cut(s) 156, 292, 490
BspPI GGATC 4 cut(s) 149, 162, 431, 498
BspT104I TTCGAA 1 cut(s) 218
BspTNI GGTCTC 1 cut(s) 349
BsrI ACTGG 2 cut(s) 103, 546
BssECI CCNNGG 1 cut(s) 477
BssMI GATC 3 cut(s) 154, 436, 503
BssNI GRCGYC 1 cut(s) 519
Bst2UI CCWGG 1 cut(s) 360
Bst6I CTCTTC 1 cut(s) 12
BstACI GRCGYC 1 cut(s) 519
BstAPI GCANNNNNTGC 1 cut(s) 200
BstBI TTCGAA 1 cut(s) 218
BstC8I GCNNGC 1 cut(s) 586
BstDEI CTNAG 8 cut(s) 51, 69, 110, 183, 353, 413, 514, 527
BstKTI GATC 3 cut(s) 157, 439, 506
BstMAI GTCTC 1 cut(s) 349
BstMBI GATC 3 cut(s) 154, 436, 503
BstMWI GCNNNNNNNGC 4 cut(s) 188, 197, 200, 516
BstNI CCWGG 1 cut(s) 360
BstNSI RCATGY 1 cut(s) 389
BstSCI CCNGG 3 cut(s) 358, 476, 477
BstV1I GCAGC 3 cut(s) 175, 187, 379
BstX2I RGATCY 2 cut(s) 154, 503
BstYI RGATCY 2 cut(s) 154, 503
BtsI GCAGTG 1 cut(s) 201
BtsIMutI CAGTG 1 cut(s) 201
Cac8I GCNNGC 1 cut(s) 586
CaiI CAGNNNCTG 1 cut(s) 200
Cfr13I GGNCC 1 cut(s) 101
Cfr9I CCCGGG 1 cut(s) 477
CseI GACGC 1 cut(s) 508
CsiI ACCWGGT 1 cut(s) 358
Csp6I GTAC 1 cut(s) 549
CviAII CATG 2 cut(s) 386, 532
CviQI GTAC 1 cut(s) 549
DdeI CTNAG 8 cut(s) 51, 69, 110, 183, 353, 413, 514, 527
DpnI GATC 3 cut(s) 156, 438, 505
DpnII GATC 3 cut(s) 154, 436, 503
DrdI GACNNNNNNGTC 1 cut(s) 541
DseDI GACNNNNNNGTC 1 cut(s) 541
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Ecl136II GAGCTC 1 cut(s) 43
Eco24I GRGCYC 1 cut(s) 45
Eco31I GGTCTC 1 cut(s) 349
Eco47I GGWCC 1 cut(s) 101
Eco53kI GAGCTC 1 cut(s) 43
Eco88I CYCGRG 1 cut(s) 477
EcoICRI GAGCTC 1 cut(s) 43
EcoRII CCWGG 1 cut(s) 358
EcoT38I GRGCYC 1 cut(s) 45
FaeI CATG 2 cut(s) 389, 535
FaqI GGGAC 2 cut(s) 109, 418
FatI CATG 2 cut(s) 385, 531
FauI CCCGC 1 cut(s) 19
Fnu4HI GCNGC 4 cut(s) 189, 192, 201, 393
FriOI GRGCYC 1 cut(s) 45
Fsp4HI GCNGC 4 cut(s) 189, 192, 201, 393
FspBI CTAG 1 cut(s) 296
GluI GCNGC 4 cut(s) 189, 192, 201, 393
HapII CCGG 2 cut(s) 478, 507
HgaI GACGC 1 cut(s) 508
Hin1I GRCGYC 1 cut(s) 519
Hin1II CATG 2 cut(s) 389, 535
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HinfI GANTC 2 cut(s) 328, 366
HpaII CCGG 2 cut(s) 478, 507
HphI GGTGA 1 cut(s) 466
Hpy166II GTNNAC 2 cut(s) 322, 365
Hpy188I TCNGA 3 cut(s) 40, 140, 416
Hpy188III TCNNGA 2 cut(s) 434, 501
Hpy8I GTNNAC 2 cut(s) 322, 365
Hpy99I CGWCG 1 cut(s) 524
HpyAV CCTTC 1 cut(s) 52
HpyCH4V TGCA 4 cut(s) 4, 281, 445, 588
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 197, 200, 516
HpyF3I CTNAG 8 cut(s) 51, 69, 110, 183, 353, 413, 514, 527
Hsp92I GRCGYC 1 cut(s) 519
Hsp92II CATG 2 cut(s) 389, 535
Kzo9I GATC 3 cut(s) 154, 436, 503
LmnI GCTCC 2 cut(s) 40, 382
Lsp1109I GCAGC 3 cut(s) 175, 187, 379
LweI GCATC 2 cut(s) 256, 268
MabI ACCWGGT 1 cut(s) 358
MaeI CTAG 1 cut(s) 296
MaeIII GTNAC 1 cut(s) 29
MalI GATC 3 cut(s) 156, 438, 505
MboI GATC 3 cut(s) 154, 436, 503
MboII GAAGA 2 cut(s) 26, 29
MflI RGATCY 2 cut(s) 154, 503
MhlI GDGCHC 1 cut(s) 45
MluCI AATT 2 cut(s) 84, 555
MlyI GAGTC 2 cut(s) 322, 360
MmeI TCCRAC 3 cut(s) 18, 60, 268
MnlI CCTC 5 cut(s) 241, 422, 547, 562, 576
MseI TTAA 1 cut(s) 342
MspI CCGG 2 cut(s) 478, 507
MspR9I CCNGG 3 cut(s) 360, 478, 479
MvaI CCWGG 1 cut(s) 360
MwoI GCNNNNNNNGC 4 cut(s) 188, 197, 200, 516
NciI CCSGG 2 cut(s) 478, 479
NdeII GATC 3 cut(s) 154, 436, 503
NlaIII CATG 2 cut(s) 389, 535
NlaIV GGNNCC 3 cut(s) 156, 292, 490
NspI RCATGY 1 cut(s) 389
NspV TTCGAA 1 cut(s) 218
PkrI GCNGC 4 cut(s) 190, 193, 202, 394
PleI GAGTC 2 cut(s) 322, 360
PpsI GAGTC 2 cut(s) 322, 360
PsiI TTATAA 1 cut(s) 560
Psp124BI GAGCTC 1 cut(s) 45
Psp6I CCWGG 1 cut(s) 358
PspGI CCWGG 1 cut(s) 358
PspN4I GGNNCC 3 cut(s) 156, 292, 490
PspPI GGNCC 1 cut(s) 101
PstNI CAGNNNCTG 1 cut(s) 200
PsuI RGATCY 2 cut(s) 154, 503
RsaI GTAC 1 cut(s) 550
RsaNI GTAC 1 cut(s) 549
SacI GAGCTC 1 cut(s) 45
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 4 cut(s) 189, 192, 201, 393
Sau3AI GATC 3 cut(s) 154, 436, 503
Sau96I GGNCC 1 cut(s) 101
SchI GAGTC 2 cut(s) 322, 360
ScrFI CCNGG 3 cut(s) 360, 478, 479
SduI GDGCHC 1 cut(s) 45
SetI ASST 7 cut(s) 45, 70, 364, 528, 539, 554, 586
SexAI ACCWGGT 1 cut(s) 358
SfaNI GCATC 2 cut(s) 256, 268
SfuI TTCGAA 1 cut(s) 218
SinI GGWCC 1 cut(s) 101
SmaI CCCGGG 1 cut(s) 479
Sse9I AATT 2 cut(s) 84, 555
SsiI CCGC 2 cut(s) 26, 191
SspMI CTAG 1 cut(s) 296
SstI GAGCTC 1 cut(s) 45
StyD4I CCNGG 3 cut(s) 358, 476, 477
TaqI TCGA 2 cut(s) 218, 522
TasI AATT 2 cut(s) 84, 555
TauI GCSGC 1 cut(s) 194
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TscAI CASTG 1 cut(s) 201
TseI GCWGC 3 cut(s) 188, 200, 392
TspMI CCCGGG 1 cut(s) 477
TspRI CASTG 1 cut(s) 201
VpaK11BI GGWCC 1 cut(s) 101
XceI RCATGY 1 cut(s) 389
XmaI CCCGGG 1 cut(s) 477
XspI CTAG 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.