Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004500

Physical Location & Seq

Genomic Coordinates Reverse (-)

27167363 .. 27169754

Gene Structure 2392 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G72190.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1122 bp

ATGACGATAATGTTTAAAATCGCAAAAGCTACGTTACGAAGCAGAAACTCAAGCGTCGCTTCGCTCTTCTACTCTATCCAAGCTCGCTCTATCTCCAGGCAAAGTAAAGTGGTTAAGATTGAGAGAATAGTTGAGAAAGAAGACATGCATGTAACTCGTGTTCTATTCTGCGGTCCTCACTTCCCTGATTCCTACAATTTCACCAGAGAATACTTGCAACCTTACCCTTTTATTAAGGTTGATGTTGTCCATTACCGCGATGTCCCTGAGGTTATTAAGAACTATCATATATGTGTGGCAATGACTATGCAAATGGATTCAAATGTTATATCTCGTGCAAGCAACATAAAGCTTATTATGCAGTACGGTGTTGGTCTCGACGGTGTTGACATTGATGCTGCTACTAAACATGGGATTAAGGTCGCTAGAATTCCTAGTGAGGGTACTGGAAATGCTGCATCTTGCTCTGAGATGGCTATATATCTAATGCTGGGACTTCTTAAGAAACAGAACGAAATGCAAATATCTCTGAGAAATAGACTACTTGGAGAACCAACCGGTGACACGCTTCTTGGTAAAACTGTATTTATCTTAGGATATGGAAACATCGGAATAGAGCTGGCTAAACGGTTGAAGCCATTTGGGTCGAGAGTAATAGCCACGAAAAGATTCTGGCCTGCTTCTATCGTCGACTCAGACTCCAGGCTAGTTGATGAGAAAGGTAGCCATGAAGACATTTACACATTCGCTGGCAAAGCCGATATAGTCGTTGTTTGTTTGAGGCTCAACAAAGAAACGGCTGAAATAGTGAATAAGGAGTTCATATGCTCAATGAAAAAGGGTGCTCTTCTTGTAAATATTGCCCGAGGTGGTCTTATTAACTATGAATCAGCTTTCCAGAATCTGGAGTCCGGTCATCTAGGAGGCCTTGGGATTGATGTGGCGTGGTCTGAGCCGTTTGATCCAAATGATCCTATTTTGAAGTTCAAAAACGTTATAATAACCCCTCATGTCGCTGGAGTTACCGAATATTCATATAGGTCCATGGCCAAGATTGTCGGAGACCTCGCGCTTCAGTTGCATGAAGGACTTCCTCTCACCGGAATCGAATTCGTCAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003008 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006081 GO:0006082 GO:0007588 GO:0008150 GO:0008152 GO:0008465 GO:0009987 GO:0016043 GO:0016491 GO:0016614 GO:0016616 GO:0016618 GO:0019752 GO:0022607 GO:0030267 GO:0031406 GO:0032501 GO:0032787 GO:0036094 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043177 GO:0043436 GO:0043648 GO:0043933 GO:0044085 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046487 GO:0046983 GO:0048037 GO:0050661 GO:0050662 GO:0051259 GO:0051287 GO:0055114 GO:0065003 GO:0070402 GO:0071704 GO:0071840 GO:0097159 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

373

Amino Acids

41.45

Weight (kDa)

8.78

Isoelectric Point (pI)

38.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2-Hacid_dh	PF00389	87 - 354	6.2e-15	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C	PF02826	161 - 339	2.7e-40	D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004500)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G72190
fragaria_vesca	FvH4_5g31330 FvH4_5g31330 FvH4_5g31330 FvH4_5g31330 FvH4_5g31330 FvH4_5g31330 FvH4_5g31330
malus_domestica	MD08G1217300.v1.1
prunus_persica	Prupe.1G556800_v2.0.a1 Prupe.1G556800_v2.0.a1 Prupe.1G556800_v2.0.a1 Prupe.1G556800_v2.0.a1 Prupe.1G556800_v2.0.a1 Prupe.1G556800_v2.0.a1
pyrus_communis	pycom08g18800
rosa_chinensis	RchiOBHm_Chr7g0226521 RchiOBHm_Chr7g0226551
rosa_laevigata	RLG00000001716 RLG00000001719
rosa_multiflora	Rmu_sc0001423.1_g000008
rosa_roxburghii	Rroxscaffold_3G00232710
rosa_rugosa	Rorug07G0237900 Rorug07G0237900 Rorug07G0238000 Rorug07G0238200
rosa_samantha	Rh7BG372200 Rh7BG372500 Rh7DG384300 Rh7DG384500
rosa_wichuraiana	Rw7G032370 Rw7G032390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	998
AccB7I	CCANNNNNTGG	1 cut(s)	904
AccI	GTMKAC	1 cut(s)	690
AccII	CGCG	2 cut(s)	258, 1070
AciI	CCGC	2 cut(s)	171, 256
AclI	AACGTT	1 cut(s)	993
AclWI	GGATC	2 cut(s)	956, 965
AcoI	YGGCCR	1 cut(s)	1047
AcsI	RAATTY	2 cut(s)	429, 1109
AcuI	CTGAAG	1 cut(s)	1058
AfaI	GTAC	2 cut(s)	365, 445
AfiI	CCNNNNNNNGG	3 cut(s)	440, 904, 1100
AflII	CTTAAG	1 cut(s)	500
AgeI	ACCGGT	1 cut(s)	557
AgsI	TTSAA	4 cut(s)	321, 634, 982, 988
AjnI	CCWGG	2 cut(s)	95, 701
AluBI	AGCT	5 cut(s)	29, 83, 352, 619, 893
AluI	AGCT	5 cut(s)	29, 83, 352, 619, 893
Alw21I	GWGCWC	1 cut(s)	847
Alw26I	GTCTC	2 cut(s)	380, 1056
AlwI	GGATC	2 cut(s)	956, 965
AlwNI	CAGNNNCTG	1 cut(s)	904
Ama87I	CYCGRG	1 cut(s)	864
AoxI	GGCC	3 cut(s)	674, 925, 1047
ApeKI	GCWGC	2 cut(s)	398, 455
ApoI	RAATTY	2 cut(s)	429, 1109
AsiGI	ACCGGT	1 cut(s)	557
Asp700I	GAANNNNTTC	2 cut(s)	668, 1089
AspLEI	GCGC	1 cut(s)	1072
AspS9I	GGNCC	2 cut(s)	173, 1041
AsuHPI	GGTGA	3 cut(s)	193, 572, 1090
AvaI	CYCGRG	1 cut(s)	864
AvaII	GGWCC	2 cut(s)	173, 1041
AxyI	CCTNAGG	1 cut(s)	267
BalI	TGGCCA	1 cut(s)	1049
BauI	CACGAG	2 cut(s)	156, 333
BbsI	GAAGAC	2 cut(s)	147, 738
Bbv12I	GWGCWC	1 cut(s)	847
BbvI	GCAGC	2 cut(s)	385, 442
BccI	CCATC	1 cut(s)	466
BceAI	ACGGC	2 cut(s)	813, 940
BcgI	CGANNNNNNTGC	2 cut(s)	137, 171
BciT130I	CCWGG	2 cut(s)	97, 703
BcoDI	GTCTC	2 cut(s)	380, 1056
BfaI	CTAG	4 cut(s)	426, 435, 707, 920
BfrI	CTTAAG	1 cut(s)	500
BisI	GCNGC	2 cut(s)	399, 456
BlsI	GCNGC	2 cut(s)	400, 457
Bme1390I	CCNGG	2 cut(s)	97, 703
Bme18I	GGWCC	2 cut(s)	173, 1041
BmeT110I	CYCGRG	1 cut(s)	864
BmgT120I	GGNCC	2 cut(s)	173, 1041
BmrFI	CCNGG	2 cut(s)	97, 703
BmsI	GCATC	2 cut(s)	385, 467
BpiI	GAAGAC	2 cut(s)	147, 738
BpmI	CTGGAG	4 cut(s)	79, 685, 926, 1038
BpuEI	CTTGAG	1 cut(s)	34
BsaI	GGTCTC	2 cut(s)	380, 1056
BsaJI	CCNNGG	3 cut(s)	865, 928, 1044
BsaWI	WCCGGW	3 cut(s)	557, 911, 1100
BsaXI	ACNNNNNCTCC	2 cut(s)	683, 713
Bsc4I	CCNNNNNNNGG	3 cut(s)	440, 904, 1100
Bse118I	RCCGGY	1 cut(s)	557
Bse1I	ACTGG	1 cut(s)	451
Bse21I	CCTNAGG	1 cut(s)	267
Bse3DI	GCAATG	1 cut(s)	306
BseBI	CCWGG	2 cut(s)	97, 703
BseDI	CCNNGG	3 cut(s)	865, 928, 1044
BseLI	CCNNNNNNNGG	3 cut(s)	440, 904, 1100
BseMI	GCAATG	1 cut(s)	306
BseMII	CTCAG	5 cut(s)	258, 459, 521, 708, 942
BseNI	ACTGG	1 cut(s)	451
BseXI	GCAGC	2 cut(s)	385, 442
BseYI	CCCAGC	1 cut(s)	490
Bsh1236I	CGCG	2 cut(s)	258, 1070
BshFI	GGCC	3 cut(s)	676, 927, 1049
BshTI	ACCGGT	1 cut(s)	557
BsiHKAI	GWGCWC	1 cut(s)	847
BsiHKCI	CYCGRG	1 cut(s)	864
BsiSI	CCGG	3 cut(s)	558, 912, 1101
BslFI	GGGAC	2 cut(s)	248, 507
BslI	CCNNNNNNNGG	3 cut(s)	440, 904, 1100
BsmAI	GTCTC	2 cut(s)	380, 1056
BsmFI	GGGAC	2 cut(s)	248, 507
BsnI	GGCC	3 cut(s)	676, 927, 1049
Bso31I	GGTCTC	2 cut(s)	380, 1056
BsoBI	CYCGRG	1 cut(s)	864
Bsp1286I	GDGCHC	1 cut(s)	847
Bsp143I	GATC	2 cut(s)	961, 970
Bsp19I	CCATGG	1 cut(s)	1044
BspACI	CCGC	2 cut(s)	171, 256
BspANI	GGCC	3 cut(s)	676, 927, 1049
BspCNI	CTCAG	5 cut(s)	259, 460, 522, 707, 943
BspFNI	CGCG	2 cut(s)	258, 1070
BspPI	GGATC	2 cut(s)	956, 965
BspQI	GCTCTTC	2 cut(s)	71, 852
BspTI	CTTAAG	1 cut(s)	500
BspTNI	GGTCTC	2 cut(s)	380, 1056
BsrDI	GCAATG	1 cut(s)	306
BsrFI	RCCGGY	1 cut(s)	557
BsrI	ACTGG	1 cut(s)	451
BssAI	RCCGGY	1 cut(s)	557
BssECI	CCNNGG	3 cut(s)	865, 928, 1044
BssMI	GATC	2 cut(s)	961, 970
BssSI	CACGAG	2 cut(s)	156, 333
BssT1I	CCWWGG	2 cut(s)	928, 1044
Bst2BI	CACGAG	2 cut(s)	156, 333
Bst2UI	CCWGG	2 cut(s)	97, 703
Bst4CI	ACNGT	4 cut(s)	368, 383, 583, 630
Bst6I	CTCTTC	2 cut(s)	71, 852
BstAFI	CTTAAG	1 cut(s)	500
BstC8I	GCNNGC	5 cut(s)	85, 340, 621, 678, 751
BstDEI	CTNAG	6 cut(s)	267, 468, 530, 592, 694, 951
BstDSI	CCRYGG	1 cut(s)	1044
BstFNI	CGCG	2 cut(s)	258, 1070
BstHHI	GCGC	1 cut(s)	1072
BstKTI	GATC	2 cut(s)	964, 973
BstMAI	GTCTC	2 cut(s)	380, 1056
BstMBI	GATC	2 cut(s)	961, 970
BstMWI	GCNNNNNNNGC	3 cut(s)	358, 755, 1078
BstNI	CCWGG	2 cut(s)	97, 703
BstNSI	RCATGY	2 cut(s)	148, 152
BstSCI	CCNGG	2 cut(s)	95, 701
BstUI	CGCG	2 cut(s)	258, 1070
BstV1I	GCAGC	2 cut(s)	385, 442
BstV2I	GAAGAC	2 cut(s)	147, 738
Bsu36I	CCTNAGG	1 cut(s)	267
BsuRI	GGCC	3 cut(s)	676, 927, 1049
BtgI	CCRYGG	1 cut(s)	1044
BtgZI	GCGATG	1 cut(s)	273
Cac8I	GCNNGC	5 cut(s)	85, 340, 621, 678, 751
CaiI	CAGNNNCTG	1 cut(s)	904
CfoI	GCGC	1 cut(s)	1072
Cfr10I	RCCGGY	1 cut(s)	557
Cfr13I	GGNCC	2 cut(s)	173, 1041
CseI	GACGC	1 cut(s)	43
Csp6I	GTAC	2 cut(s)	364, 444
CspAI	ACCGGT	1 cut(s)	557
CviAII	CATG	7 cut(s)	145, 149, 410, 728, 1010, 1045, 1082
CviQI	GTAC	2 cut(s)	364, 444
DdeI	CTNAG	6 cut(s)	267, 468, 530, 592, 694, 951
DpnI	GATC	2 cut(s)	963, 972
DpnII	GATC	2 cut(s)	961, 970
DraI	TTTAAA	1 cut(s)	16
EaeI	YGGCCR	1 cut(s)	1047
Eam1104I	CTCTTC	2 cut(s)	71, 852
EarI	CTCTTC	2 cut(s)	71, 852
Eco130I	CCWWGG	2 cut(s)	928, 1044
Eco147I	AGGCCT	1 cut(s)	927
Eco31I	GGTCTC	2 cut(s)	380, 1056
Eco47I	GGWCC	2 cut(s)	173, 1041
Eco57I	CTGAAG	1 cut(s)	1058
Eco81I	CCTNAGG	1 cut(s)	267
Eco88I	CYCGRG	1 cut(s)	864
EcoRI	GAATTC	2 cut(s)	429, 1109
EcoRII	CCWGG	2 cut(s)	95, 701
EcoT14I	CCWWGG	2 cut(s)	928, 1044
EcoT22I	ATGCAT	1 cut(s)	150
ErhI	CCWWGG	2 cut(s)	928, 1044
FaeI	CATG	7 cut(s)	148, 152, 413, 731, 1013, 1048, 1085
FalI	AAGNNNNNCTT	2 cut(s)	43, 75
FaqI	GGGAC	2 cut(s)	248, 507
FatI	CATG	7 cut(s)	144, 148, 409, 727, 1009, 1044, 1081
FauNDI	CATATG	1 cut(s)	824
FblI	GTMKAC	1 cut(s)	690
Fnu4HI	GCNGC	2 cut(s)	399, 456
Fsp4HI	GCNGC	2 cut(s)	399, 456
FspBI	CTAG	4 cut(s)	426, 435, 707, 920
GlaI	GCGC	1 cut(s)	1071
GluI	GCNGC	2 cut(s)	399, 456
GsaI	CCCAGC	1 cut(s)	494
GsuI	CTGGAG	4 cut(s)	79, 685, 926, 1038
HaeIII	GGCC	3 cut(s)	676, 927, 1049
HapII	CCGG	3 cut(s)	558, 912, 1101
HgaI	GACGC	1 cut(s)	43
HhaI	GCGC	1 cut(s)	1072
Hin1II	CATG	7 cut(s)	148, 152, 413, 731, 1013, 1048, 1085
Hin6I	GCGC	1 cut(s)	1070
HinP1I	GCGC	1 cut(s)	1070
HincII	GTYRAC	2 cut(s)	388, 691
HindII	GTYRAC	2 cut(s)	388, 691
HindIII	AAGCTT	1 cut(s)	350
HinfI	GANTC	9 cut(s)	188, 317, 669, 692, 698, 887, 901, 908, 1104
HpaII	CCGG	3 cut(s)	558, 912, 1101
HphI	GGTGA	3 cut(s)	193, 572, 1090
Hpy166II	GTNNAC	2 cut(s)	388, 691
Hpy188I	TCNGA	6 cut(s)	469, 531, 611, 697, 952, 1061
Hpy188III	TCNNGA	4 cut(s)	377, 648, 898, 905
Hpy8I	GTNNAC	2 cut(s)	388, 691
Hpy99I	CGWCG	3 cut(s)	59, 383, 692
HpyAV	CCTTC	1 cut(s)	1079
HpyCH4III	ACNGT	4 cut(s)	368, 383, 583, 630
HpyCH4IV	ACGT	2 cut(s)	32, 993
HpyCH4V	TGCA	8 cut(s)	148, 217, 310, 338, 361, 458, 520, 1081
HpyF10VI	GCNNNNNNNGC	3 cut(s)	358, 755, 1078
HpyF3I	CTNAG	6 cut(s)	267, 468, 530, 592, 694, 951
HpySE526I	ACGT	2 cut(s)	32, 993
Hsp92II	CATG	7 cut(s)	148, 152, 413, 731, 1013, 1048, 1085
HspAI	GCGC	1 cut(s)	1070
Kzo9I	GATC	2 cut(s)	961, 970
LguI	GCTCTTC	2 cut(s)	71, 852
Lsp1109I	GCAGC	2 cut(s)	385, 442
LweI	GCATC	2 cut(s)	385, 467
MaeI	CTAG	4 cut(s)	426, 435, 707, 920
MaeII	ACGT	2 cut(s)	32, 993
MaeIII	GTNAC	4 cut(s)	33, 151, 560, 1021
MalI	GATC	2 cut(s)	963, 972
MboI	GATC	2 cut(s)	961, 970
MboII	GAAGA	4 cut(s)	58, 152, 743, 839
MhlI	GDGCHC	1 cut(s)	847
MlsI	TGGCCA	1 cut(s)	1049
MluCI	AATT	4 cut(s)	196, 429, 1109, 1117
MluNI	TGGCCA	1 cut(s)	1049
MlyI	GAGTC	3 cut(s)	686, 692, 917
MmeI	TCCRAC	1 cut(s)	1039
MnlI	CCTC	9 cut(s)	186, 262, 433, 774, 860, 917, 1017, 1076, 1104
Mox20I	TGGCCA	1 cut(s)	1049
Mph1103I	ATGCAT	1 cut(s)	150
MroXI	GAANNNNTTC	2 cut(s)	668, 1089
MscI	TGGCCA	1 cut(s)	1049
MseI	TTAA	8 cut(s)	15, 114, 234, 276, 417, 501, 879, 1120
MslI	CAYNNNNRTG	1 cut(s)	291
Msp20I	TGGCCA	1 cut(s)	1049
MspCI	CTTAAG	1 cut(s)	500
MspI	CCGG	3 cut(s)	558, 912, 1101
MspR9I	CCNGG	2 cut(s)	97, 703
MvaI	CCWGG	2 cut(s)	97, 703
MvnI	CGCG	2 cut(s)	258, 1070
MwoI	GCNNNNNNNGC	3 cut(s)	358, 755, 1078
NcoI	CCATGG	1 cut(s)	1044
NdeI	CATATG	1 cut(s)	824
NdeII	GATC	2 cut(s)	961, 970
NlaIII	CATG	7 cut(s)	148, 152, 413, 731, 1013, 1048, 1085
NmuCI	GTSAC	1 cut(s)	560
NsiI	ATGCAT	1 cut(s)	150
NspI	RCATGY	2 cut(s)	148, 152
PceI	AGGCCT	1 cut(s)	927
PciSI	GCTCTTC	2 cut(s)	71, 852
PdmI	GAANNNNTTC	2 cut(s)	668, 1089
PfeI	GAWTC	6 cut(s)	188, 317, 669, 887, 901, 1104
PflMI	CCANNNNNTGG	1 cut(s)	904
PinAI	ACCGGT	1 cut(s)	557
PkrI	GCNGC	2 cut(s)	400, 457
PleI	GAGTC	3 cut(s)	686, 692, 916
PpsI	GAGTC	3 cut(s)	686, 692, 916
PsiI	TTATAA	1 cut(s)	998
Psp1406I	AACGTT	1 cut(s)	993
Psp6I	CCWGG	2 cut(s)	95, 701
PspFI	CCCAGC	1 cut(s)	490
PspGI	CCWGG	2 cut(s)	95, 701
PspPI	GGNCC	2 cut(s)	173, 1041
PsrI	GAACNNNNNNTAC	2 cut(s)	144, 176
PstNI	CAGNNNCTG	1 cut(s)	904
RsaI	GTAC	2 cut(s)	365, 445
RsaNI	GTAC	2 cut(s)	364, 444
RseI	CAYNNNNRTG	1 cut(s)	291
SalI	GTCGAC	1 cut(s)	689
SapI	GCTCTTC	2 cut(s)	71, 852
SaqAI	TTAA	8 cut(s)	15, 114, 234, 276, 417, 501, 879, 1120
SatI	GCNGC	2 cut(s)	399, 456
Sau3AI	GATC	2 cut(s)	961, 970
Sau96I	GGNCC	2 cut(s)	173, 1041
SchI	GAGTC	3 cut(s)	686, 692, 917
ScrFI	CCNGG	2 cut(s)	97, 703
SduI	GDGCHC	1 cut(s)	847
SfaNI	GCATC	2 cut(s)	385, 467
SinI	GGWCC	2 cut(s)	173, 1041
SmiMI	CAYNNNNRTG	1 cut(s)	291
SmlI	CTYRAG	2 cut(s)	49, 500
SmoI	CTYRAG	2 cut(s)	49, 500
Sse9I	AATT	4 cut(s)	196, 429, 1109, 1117
SseBI	AGGCCT	1 cut(s)	927
SsiI	CCGC	2 cut(s)	171, 256
SspI	AATATT	2 cut(s)	859, 1031
SspMI	CTAG	4 cut(s)	426, 435, 707, 920
StuI	AGGCCT	1 cut(s)	927
StyD4I	CCNGG	2 cut(s)	95, 701
StyI	CCWWGG	2 cut(s)	928, 1044
TaaI	ACNGT	4 cut(s)	368, 383, 583, 630
TaiI	ACGT	2 cut(s)	35, 996
TaqI	TCGA	4 cut(s)	378, 647, 690, 1107
TasI	AATT	4 cut(s)	196, 429, 1109, 1117
TfiI	GAWTC	6 cut(s)	188, 317, 669, 887, 901, 1104
Tru1I	TTAA	8 cut(s)	15, 114, 234, 276, 417, 501, 879, 1120
Tru9I	TTAA	8 cut(s)	15, 114, 234, 276, 417, 501, 879, 1120
TseFI	GTSAC	1 cut(s)	560
TseI	GCWGC	2 cut(s)	398, 455
Tsp45I	GTSAC	1 cut(s)	560
TspDTI	ATGAA	6 cut(s)	744, 811, 848, 900, 1023, 1098
Van91I	CCANNNNNTGG	1 cut(s)	904
Vha464I	CTTAAG	1 cut(s)	500
VpaK11BI	GGWCC	2 cut(s)	173, 1041
XapI	RAATTY	2 cut(s)	429, 1109
XceI	RCATGY	2 cut(s)	148, 152
XmiI	GTMKAC	1 cut(s)	690
XmnI	GAANNNNTTC	2 cut(s)	668, 1089
XspI	CTAG	4 cut(s)	426, 435, 707, 920
Zsp2I	ATGCAT	1 cut(s)	150

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.